Pay0003985 (gene) Melon (Payzawat) v1

Overview
NamePay0003985
Typegene
OrganismCucumis melo L. var. inodorus cv. Payzawat (Melon (Payzawat) v1)
Description26S proteasome regulatory subunit 8 homolog A-like
Locationchr05: 2800242 .. 2804198 (-)
RNA-Seq ExpressionPay0003985
SyntenyPay0003985
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACGATGGAGTTAGACACCAAGCTACCGGAGGCCATAAATGAAGAGGCGTGCTCCGCCAAACCGGCGACAAAGCACGGCGAAGGTCTTAGGCACTATTATCTTCAACACATTCAGGACCTCCAGCTTCAACTCCGCCTCAAAACTCATAATCTCAATCGTCTTGAAGCTCAGCGAAACGAGCTCAACTCTAGAGGTAACACTGATTACTTAACCGTATCTCCTTTTCTCCAATTATAGGGTTTGGAATTGTAGTAGCTGGTAGTTTTATAGATTTTGGTCGGAGAGCTATCTCGATCAGCTTACCTTTCTTAGATCAACGATGATTTGCATTTTCGTGTGGTGAAATTTTGTTTTCTTGCAGACTGGAATGTTTTTCTTCGATTGTATTGAACGTTTCGATAAAACTTCGGGTCTCTAGATTAAGAAACCGACTCCAATTCAGAACTAGGGTTCTTGATTATTTTAAAGTAGGATATGGATAATTCTTTTCTTTTTTATGCGTCGTGGAAATGTGAAATAGTTAGATGAATCAAGCAGACAACATGAGTTTTGTGTTAGATATCAATACGTTTTGGTAAATCATTTTTGTGTTCTTTGTTAGGATCGTGAATGGTTTAGGATTATATTCTGTTGATAAAACTAGTTGTACTGGTTGCAATTTTGTAGTGAGGTTGCTTCGTGAAGAACTTCAGCTGCTTCAAGAACCTGGATCTTATGTTGGCGAAGTGGTCAAAGTCATGGGAAAGAATAAGGTTTTAGTGAAGGTGAGTGATTATAGGAAAGTAATTTCTGTTTTTCTTGAAGACTTAGCCACCAGTTACATCCACATTCCCGTGTGTGTTATCTGTACGAAGTTACCTATATTGGTTACAAAATGGATATGCAGGTGGAGATTTTCTGTTGTAAGATGTATCGTTCAGAATGGTTTTTATTTTTCCACTAGAGTGACTGGAAGAATTGATAATATTATATATGTTGTAAAGAGGATATGTGGTCGATAACTTCCATTTCAACCTTTAATGAGTAGTAACGTATAATTTTCATATTTACTTTGTACAAAATGATCTCACATATTTAAAACCATGTCTATTTTGCATAAAAAATATTAGGTTGTTTTGAACAGACTTTTAGTTTTAATCCTCCAATTCATTGCATTCTTTTGGAAAAAGGCAATGTTCTCCACAATATTGACTTCTAATTTTTTTTAATAACTCTACCAGTATATGCATTTTTTCCCGTGCTGGTTGGATTTGTGGTTTACATTTAAGCTTGCATAGTTTTCTTTTCTATAAACTTCTGTCCTCAAAGCATATTATTTAGTTTGGTAGTCATATGACAAGCGTTCAATTCCAACAGCTCATAATATTACACTTCATTCTTTCACACCTAATCTCCAATTCTTCGTTACAAGGTCGCATCCAGCAAGGGCAATATAATTGTGTAATATAATAAAAAAGCAATTTTATTCATCATCAAAACATCCACCCCATCAACTTATGTCTGCCTTCTCCGAAATGACAGTCACTTTCTTCATCTTATATCTGCAATCAGCAATTAGTAGATCCGATTTAGTAGTAATTTATGAAAGTCTGAAAACAGGTCCCTGATTCTACCTTATACATGATTGGGTCTTCGCATCATCCAAACAAGGATTAATATAGAGGTATATCTCTCTATCGTTTTTCATTCCCTGACTTACCCTCATGCTTGTAGCGTGTTGATTCATCTCTATCTTTCCACCTTCACTTCGTTCGTACTCCCTCTTTTCTTTTCTCTACCCGCCTGGTGCCCAGCGCGAGGTTTGGTTGCTTTGTCCCTCGTACTGTTTCGCAAAAGTAAAGTTGGCCAGTACAGGAATCAAACAAAACGGGAGAATGCCTTTACAGGCCATGTCACATACGCGCAATGGAGGCTTACTCCGCATTGTAACTCTGGAGCACCAAGCCGATATACCGGGCGATAGGCTACTACAGACTTATATTAGAAACTTGAAATCGCTATTTCACTTTTTCATATTGAATTCTTCATTAGGTCATTGAGCTTCCAATCAAGCATCCAGAGTTATTCGAAAGTCTTGGAATAGCTCAACCAAAGGTAAAATCGAGTGTGCAGTGGGCTGTTTCTAGTTGCTGATATACTTTCCTCTAATCTCACTCTCTTTTTCATTCTTAAATCATGCGCAGGGTGTCTTGCTATATGGGCCTCCTGGTACTGGGAAAACACTGTTGGCTCGTGCAGTATCTCTTTCTAGGTTTTCTTATAATCATATTTGAGAGGTTCTAGATGTTAGACTTTTTGATGTATTTTTTCTTTAATTCTTTTTCTATCTTTATTATTGATCATGTTTCATTACAGAGTTTAATGTCTTTCTTATCTTATTTTTTCTCTATCTTTTAATTGTCTGAGCTGATCATTCAACCTATACCATCTTATTATAGTATATTATATGTATTTGTGATAAATAAGGAAGGGCATAATCTGATTGTTAACCATGCTCTTAATGGCATCCTTATTATATTCACTGAAAAATAATTCAATGGAAAATGTGATTTCTTGACTTCTATGTTTTTGTTACTTCATGCTTTCTCAGAATCACGTGTATTTTTGTAATGTCAACTTATGAATAAAACACGTTTTTTAATACACTTCTGCAGGGAACATGCTCCGTCTATCATTTTCATGGATGAAATTGATAGTATTGGGTCAGCTCGTATGGAATCTGGGTCTGGTAATAGTGATAGTGAGGTGCAGAGAACTATGCTGGAACTTCTTAACCAACTGGATGGATTTGAAGCGTCTAACAAGATCAAGGTTTGTATTGCTGAATTTTGTACAATAGTTTATAAGAAACTAGAAATATGTTATGCTTGTTGATTTGATGTACTAACTACCAGCTTATCGACAGGTTCTGATGGCCACAAATCGGATTGATATTCTTGATCAGGCTCTTCTAAGACCTGGACGAATTGATAGAAAAATAGAGTTCCCAAATCCTAATGAGGAGGTACATGACATTGCTAAACTTCTACTTGGAAATTTATTAGCAACATGTAGGTTTGTTCATTAACAAACATTAAGGGTAAAAGAAAATGGAACTTAAGTGTAGTTCTATCTAAATCTTTAATAGCCAAGGGTGCTTCCTTTTGGATCCTTCAGCCATTTTATTCTTTATAAGGAGGTCCATCGGGGAGTTTCCAGGGACAGTGACATGTTGTTCTAGAGGTGTCAAAATGGACAAGAACAGCCTGAGTGAGACGACGGACCTAATCACAAACTTTCAAAAGAATCTTAGGCTGTCCTTATAAATATGGTGGCATGTCTGCAGCTGAAACTCCATGGATCTTATACATATTCATGCAAGCCATATAATAAAAAACTACAGTATATATTACTTTGGGTCCTGAAAGATATATTTTAGTTTAATACTCAGAACTTAACTTCAAGAACAGAGTTGAAATATAGGTTCCTATTTATGAAATAAAGTTAAATTACAAGTTTAGTCCACAAATTTTGTTTCAAATAGGCCTCCAAACTTTGGGTCAAACTTTTGATTGTTTGTGTAATAGCCCTTTAGTATCAATTGTCTTTTAGTATGATGACCTTGTGGTATATCTTCTGCAATGCAGTCTCGTTTTGATATCTTGAAAATACATTCGAGAAAAATGAATTTAATGCGGGGGATCGACTTGAAAAAGATTGCTGAGAAGATGAATGGTGCATCCGGTGCTGAACTTAAGGTCAGTTAGATTTCTACACGAAACATTCCTTTTTTTTTCTGCTTCAGGTGAACGGAACTTCAATGTCAACCTCATTTCATCCTTTCAGGCTGTCTGCACAGAAGCTGGAATGTTTGCTCTAAGAGAAAGAAGGGTTCATGTGACACAAGAAGATTTCGAGATGGCAGTTGCAAAGGTTATGAAGAAAGACACAGATAAGAACATGTCATTGTGA

mRNA sequence

ATGGCGACGATGGAGTTAGACACCAAGCTACCGGAGGCCATAAATGAAGAGGCGTGCTCCGCCAAACCGGCGACAAAGCACGGCGAAGGTCTTAGGCACTATTATCTTCAACACATTCAGGACCTCCAGCTTCAACTCCGCCTCAAAACTCATAATCTCAATCGTCTTGAAGCTCAGCGAAACGAGCTCAACTCTAGAGTGAGGTTGCTTCGTGAAGAACTTCAGCTGCTTCAAGAACCTGGATCTTATGTTGGCGAAGTGGTCAAAGTCATGGGAAAGAATAAGGTTTTAGTGAAGGTCATTGAGCTTCCAATCAAGCATCCAGAGTTATTCGAAAGTCTTGGAATAGCTCAACCAAAGGGTGTCTTGCTATATGGGCCTCCTGGTACTGGGAAAACACTGTTGGCTCGTGCAGTATCTCTTTCTAGGGAACATGCTCCGTCTATCATTTTCATGGATGAAATTGATAGTATTGGGTCAGCTCGTATGGAATCTGGGTCTGGTAATAGTGATAGTGAGGTGCAGAGAACTATGCTGGAACTTCTTAACCAACTGGATGGATTTGAAGCGTCTAACAAGATCAAGGTTCTGATGGCCACAAATCGGATTGATATTCTTGATCAGGCTCTTCTAAGACCTGGACGAATTGATAGAAAAATAGAGTTCCCAAATCCTAATGAGGAGTCTCGTTTTGATATCTTGAAAATACATTCGAGAAAAATGAATTTAATGCGGGGGATCGACTTGAAAAAGATTGCTGAGAAGATGAATGGTGCATCCGGTGCTGAACTTAAGGCTGTCTGCACAGAAGCTGGAATGTTTGCTCTAAGAGAAAGAAGGGTTCATGTGACACAAGAAGATTTCGAGATGGCAGTTGCAAAGGTTATGAAGAAAGACACAGATAAGAACATGTCATTGTGA

Coding sequence (CDS)

ATGGCGACGATGGAGTTAGACACCAAGCTACCGGAGGCCATAAATGAAGAGGCGTGCTCCGCCAAACCGGCGACAAAGCACGGCGAAGGTCTTAGGCACTATTATCTTCAACACATTCAGGACCTCCAGCTTCAACTCCGCCTCAAAACTCATAATCTCAATCGTCTTGAAGCTCAGCGAAACGAGCTCAACTCTAGAGTGAGGTTGCTTCGTGAAGAACTTCAGCTGCTTCAAGAACCTGGATCTTATGTTGGCGAAGTGGTCAAAGTCATGGGAAAGAATAAGGTTTTAGTGAAGGTCATTGAGCTTCCAATCAAGCATCCAGAGTTATTCGAAAGTCTTGGAATAGCTCAACCAAAGGGTGTCTTGCTATATGGGCCTCCTGGTACTGGGAAAACACTGTTGGCTCGTGCAGTATCTCTTTCTAGGGAACATGCTCCGTCTATCATTTTCATGGATGAAATTGATAGTATTGGGTCAGCTCGTATGGAATCTGGGTCTGGTAATAGTGATAGTGAGGTGCAGAGAACTATGCTGGAACTTCTTAACCAACTGGATGGATTTGAAGCGTCTAACAAGATCAAGGTTCTGATGGCCACAAATCGGATTGATATTCTTGATCAGGCTCTTCTAAGACCTGGACGAATTGATAGAAAAATAGAGTTCCCAAATCCTAATGAGGAGTCTCGTTTTGATATCTTGAAAATACATTCGAGAAAAATGAATTTAATGCGGGGGATCGACTTGAAAAAGATTGCTGAGAAGATGAATGGTGCATCCGGTGCTGAACTTAAGGCTGTCTGCACAGAAGCTGGAATGTTTGCTCTAAGAGAAAGAAGGGTTCATGTGACACAAGAAGATTTCGAGATGGCAGTTGCAAAGGTTATGAAGAAAGACACAGATAAGAACATGTCATTGTGA

Protein sequence

MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQRNELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVSLSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNKIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIAEKMNGASGAELKAVCTEAGMFALRERRVHVTQEDFEMAVAKVMKKDTDKNMSL
Homology
BLAST of Pay0003985 vs. ExPASy Swiss-Prot
Match: Q9C5U3 (26S proteasome regulatory subunit 8 homolog A OS=Arabidopsis thaliana OX=3702 GN=RPT6A PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 3.5e-134
Identity = 277/414 (66.91%), Postives = 290/414 (70.05%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAK-PATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQ 60
           MA + +D++ PE   EE C+ K  A K GEGL+ YYLQHI +LQ QLR KT+NLNRLEAQ
Sbjct: 1   MAAVGVDSRRPETAMEETCNVKGAAAKQGEGLKQYYLQHIHELQRQLRQKTNNLNRLEAQ 60

Query: 61  RNELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK-------------------- 120
           RNELNSRVR+LREELQLLQEPGSYVGEVVKVMGKNKVLVK                    
Sbjct: 61  RNELNSRVRMLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKI 120

Query: 121 -------------------------------------------------------VIELP 180
                                                                  VIELP
Sbjct: 121 TPSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELP 180

Query: 181 IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------ 240
           IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                        
Sbjct: 181 IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGS 240

Query: 241 --------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASN 300
                   ++REHAPSIIFMDEIDSIGSARMESGSGN DSEVQRTMLELLNQLDGFEASN
Sbjct: 241 RMVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNGDSEVQRTMLELLNQLDGFEASN 300

Query: 301 KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI 307
           KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI
Sbjct: 301 KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI 360

BLAST of Pay0003985 vs. ExPASy Swiss-Prot
Match: Q94BQ2 (26S proteasome regulatory subunit 8 homolog B OS=Arabidopsis thaliana OX=3702 GN=RPT6B PE=1 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 4.1e-130
Identity = 271/414 (65.46%), Postives = 285/414 (68.84%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAK-PATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQ 60
           MA + ++ + P    EE C+ K  A K GEGL  YYLQH+ +LQ   R K++NLNRLEAQ
Sbjct: 1   MAAVGVEARPPVTAMEETCNVKGAAAKQGEGLNKYYLQHLDELQRLQREKSYNLNRLEAQ 60

Query: 61  RNELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK-------------------- 120
           RNELNSRVR+LREELQLLQEPGSYVGEVVKVMGKNKVLVK                    
Sbjct: 61  RNELNSRVRMLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKL 120

Query: 121 -------------------------------------------------------VIELP 180
                                                                  VIELP
Sbjct: 121 TPSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELP 180

Query: 181 IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------ 240
           IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                        
Sbjct: 181 IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGS 240

Query: 241 --------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASN 300
                   ++REHAPSIIFMDEIDSIGSARMESGSGN DSEVQRTMLELLNQLDGFEASN
Sbjct: 241 RMVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNGDSEVQRTMLELLNQLDGFEASN 300

Query: 301 KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI 307
           KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI
Sbjct: 301 KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI 360

BLAST of Pay0003985 vs. ExPASy Swiss-Prot
Match: P62194 (26S proteasome regulatory subunit 8 OS=Bos taurus OX=9913 GN=PSMC5 PE=2 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 4.8e-107
Identity = 229/388 (59.02%), Postives = 255/388 (65.72%), Query Frame = 0

Query: 26  KHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQRNELNSRVRLLREELQLLQEPGSYVG 85
           K G GLR YYL  I++LQL +  K+ NL RL+AQRNELN++VRLLREELQLLQE GSYVG
Sbjct: 15  KAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVG 74

Query: 86  EVVKVMGKNKVLVK---------------------------------------------- 145
           EVV+ M K KVLVK                                              
Sbjct: 75  EVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPL 134

Query: 146 -----------------------------VIELPIKHPELFESLGIAQPKGVLLYGPPGT 205
                                        VIELP+KHPELFE+LGIAQPKGVLLYGPPGT
Sbjct: 135 VSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGT 194

Query: 206 GKTLLARAVS--------------------------------LSREHAPSIIFMDEIDSI 265
           GKTLLARAV+                                ++REHAPSIIFMDEIDSI
Sbjct: 195 GKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSI 254

Query: 266 GSARMESGSGNSDSEVQRTMLELLNQLDGFEASNKIKVLMATNRIDILDQALLRPGRIDR 307
           GS+R+E GSG  DSEVQRTMLELLNQLDGFEA+  IKV+MATNRIDILD ALLRPGRIDR
Sbjct: 255 GSSRLEGGSG-GDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDR 314

BLAST of Pay0003985 vs. ExPASy Swiss-Prot
Match: P62195 (26S proteasome regulatory subunit 8 OS=Homo sapiens OX=9606 GN=PSMC5 PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 4.8e-107
Identity = 229/388 (59.02%), Postives = 255/388 (65.72%), Query Frame = 0

Query: 26  KHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQRNELNSRVRLLREELQLLQEPGSYVG 85
           K G GLR YYL  I++LQL +  K+ NL RL+AQRNELN++VRLLREELQLLQE GSYVG
Sbjct: 15  KAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVG 74

Query: 86  EVVKVMGKNKVLVK---------------------------------------------- 145
           EVV+ M K KVLVK                                              
Sbjct: 75  EVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPL 134

Query: 146 -----------------------------VIELPIKHPELFESLGIAQPKGVLLYGPPGT 205
                                        VIELP+KHPELFE+LGIAQPKGVLLYGPPGT
Sbjct: 135 VSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGT 194

Query: 206 GKTLLARAVS--------------------------------LSREHAPSIIFMDEIDSI 265
           GKTLLARAV+                                ++REHAPSIIFMDEIDSI
Sbjct: 195 GKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSI 254

Query: 266 GSARMESGSGNSDSEVQRTMLELLNQLDGFEASNKIKVLMATNRIDILDQALLRPGRIDR 307
           GS+R+E GSG  DSEVQRTMLELLNQLDGFEA+  IKV+MATNRIDILD ALLRPGRIDR
Sbjct: 255 GSSRLEGGSG-GDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDR 314

BLAST of Pay0003985 vs. ExPASy Swiss-Prot
Match: P62196 (26S proteasome regulatory subunit 8 OS=Mus musculus OX=10090 GN=Psmc5 PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 4.8e-107
Identity = 229/388 (59.02%), Postives = 255/388 (65.72%), Query Frame = 0

Query: 26  KHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQRNELNSRVRLLREELQLLQEPGSYVG 85
           K G GLR YYL  I++LQL +  K+ NL RL+AQRNELN++VRLLREELQLLQE GSYVG
Sbjct: 15  KAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVG 74

Query: 86  EVVKVMGKNKVLVK---------------------------------------------- 145
           EVV+ M K KVLVK                                              
Sbjct: 75  EVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPL 134

Query: 146 -----------------------------VIELPIKHPELFESLGIAQPKGVLLYGPPGT 205
                                        VIELP+KHPELFE+LGIAQPKGVLLYGPPGT
Sbjct: 135 VSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGT 194

Query: 206 GKTLLARAVS--------------------------------LSREHAPSIIFMDEIDSI 265
           GKTLLARAV+                                ++REHAPSIIFMDEIDSI
Sbjct: 195 GKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSI 254

Query: 266 GSARMESGSGNSDSEVQRTMLELLNQLDGFEASNKIKVLMATNRIDILDQALLRPGRIDR 307
           GS+R+E GSG  DSEVQRTMLELLNQLDGFEA+  IKV+MATNRIDILD ALLRPGRIDR
Sbjct: 255 GSSRLEGGSG-GDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDR 314

BLAST of Pay0003985 vs. ExPASy TrEMBL
Match: A0A5A7V082 (26S protease regulatory subunit 8-like protein A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001470 PE=3 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 4.5e-148
Identity = 303/413 (73.37%), Postives = 306/413 (74.09%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60
           MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR
Sbjct: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60

Query: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK--------------------- 120
           NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK                     
Sbjct: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKIT 120

Query: 121 ------------------------------------------------------VIELPI 180
                                                                 VIELPI
Sbjct: 121 PSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 180

Query: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------- 240
           KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                         
Sbjct: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSR 240

Query: 241 -------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300
                  ++REHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK
Sbjct: 241 MVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300

Query: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 307
           IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA
Sbjct: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 360

BLAST of Pay0003985 vs. ExPASy TrEMBL
Match: A0A1S3C7Z1 (26S protease regulatory subunit 8 homolog A-like OS=Cucumis melo OX=3656 GN=LOC103497510 PE=3 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 4.5e-148
Identity = 303/413 (73.37%), Postives = 306/413 (74.09%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60
           MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR
Sbjct: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60

Query: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK--------------------- 120
           NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK                     
Sbjct: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKIT 120

Query: 121 ------------------------------------------------------VIELPI 180
                                                                 VIELPI
Sbjct: 121 PSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 180

Query: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------- 240
           KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                         
Sbjct: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSR 240

Query: 241 -------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300
                  ++REHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK
Sbjct: 241 MVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300

Query: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 307
           IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA
Sbjct: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 360

BLAST of Pay0003985 vs. ExPASy TrEMBL
Match: A0A0A0LKW6 (AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G005970 PE=3 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.1e-146
Identity = 300/413 (72.64%), Postives = 305/413 (73.85%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60
           MATMELDTKLPEAI+E+ CSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR
Sbjct: 1   MATMELDTKLPEAISEDTCSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60

Query: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK--------------------- 120
           NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK                     
Sbjct: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKIT 120

Query: 121 ------------------------------------------------------VIELPI 180
                                                                 VIELPI
Sbjct: 121 PSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 180

Query: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------- 240
           KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                         
Sbjct: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSR 240

Query: 241 -------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300
                  ++REHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK
Sbjct: 241 MVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300

Query: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 307
           IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA
Sbjct: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 360

BLAST of Pay0003985 vs. ExPASy TrEMBL
Match: A0A6J1KJI1 (26S proteasome regulatory subunit 8 homolog A-like OS=Cucurbita maxima OX=3661 GN=LOC111493757 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 5.1e-144
Identity = 297/413 (71.91%), Postives = 301/413 (72.88%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60
           MATMELDT LPEA  EE CSAKPATK GEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR
Sbjct: 1   MATMELDTTLPEATLEETCSAKPATKQGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60

Query: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK--------------------- 120
           NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK                     
Sbjct: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKIT 120

Query: 121 ------------------------------------------------------VIELPI 180
                                                                 VIELPI
Sbjct: 121 PSARVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 180

Query: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------- 240
           KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                         
Sbjct: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSR 240

Query: 241 -------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300
                  ++REH+PSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK
Sbjct: 241 MVRELFVMAREHSPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300

Query: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 307
           IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA
Sbjct: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 360

BLAST of Pay0003985 vs. ExPASy TrEMBL
Match: A0A6J1G431 (26S proteasome regulatory subunit 8 homolog A-like OS=Cucurbita moschata OX=3662 GN=LOC111450610 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 6.7e-144
Identity = 297/413 (71.91%), Postives = 300/413 (72.64%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60
           MATME DT LPEA  EE CSAKPATK GEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR
Sbjct: 1   MATMEFDTTLPEATLEETCSAKPATKQGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60

Query: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK--------------------- 120
           NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK                     
Sbjct: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKIT 120

Query: 121 ------------------------------------------------------VIELPI 180
                                                                 VIELPI
Sbjct: 121 PLTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 180

Query: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------- 240
           KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                         
Sbjct: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSR 240

Query: 241 -------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300
                  ++REHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK
Sbjct: 241 MVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300

Query: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 307
           IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA
Sbjct: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 360

BLAST of Pay0003985 vs. NCBI nr
Match: KAA0061582.1 (26S protease regulatory subunit 8-like protein A-like [Cucumis melo var. makuwa])

HSP 1 Score: 533.9 bits (1374), Expect = 9.2e-148
Identity = 303/413 (73.37%), Postives = 306/413 (74.09%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60
           MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR
Sbjct: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60

Query: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK--------------------- 120
           NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK                     
Sbjct: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKIT 120

Query: 121 ------------------------------------------------------VIELPI 180
                                                                 VIELPI
Sbjct: 121 PSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 180

Query: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------- 240
           KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                         
Sbjct: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSR 240

Query: 241 -------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300
                  ++REHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK
Sbjct: 241 MVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300

Query: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 307
           IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA
Sbjct: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 360

BLAST of Pay0003985 vs. NCBI nr
Match: XP_008457948.1 (PREDICTED: 26S protease regulatory subunit 8 homolog A-like [Cucumis melo])

HSP 1 Score: 533.9 bits (1374), Expect = 9.2e-148
Identity = 303/413 (73.37%), Postives = 306/413 (74.09%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60
           MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR
Sbjct: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60

Query: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK--------------------- 120
           NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK                     
Sbjct: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKIT 120

Query: 121 ------------------------------------------------------VIELPI 180
                                                                 VIELPI
Sbjct: 121 PSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 180

Query: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------- 240
           KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                         
Sbjct: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSR 240

Query: 241 -------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300
                  ++REHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK
Sbjct: 241 MVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300

Query: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 307
           IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA
Sbjct: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 360

BLAST of Pay0003985 vs. NCBI nr
Match: XP_004139327.1 (26S proteasome regulatory subunit 8 homolog A [Cucumis sativus] >KGN60651.1 hypothetical protein Csa_019278 [Cucumis sativus])

HSP 1 Score: 529.3 bits (1362), Expect = 2.3e-146
Identity = 300/413 (72.64%), Postives = 305/413 (73.85%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60
           MATMELDTKLPEAI+E+ CSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR
Sbjct: 1   MATMELDTKLPEAISEDTCSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60

Query: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK--------------------- 120
           NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK                     
Sbjct: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKIT 120

Query: 121 ------------------------------------------------------VIELPI 180
                                                                 VIELPI
Sbjct: 121 PSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 180

Query: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------- 240
           KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                         
Sbjct: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSR 240

Query: 241 -------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300
                  ++REHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK
Sbjct: 241 MVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300

Query: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 307
           IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA
Sbjct: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 360

BLAST of Pay0003985 vs. NCBI nr
Match: XP_038891238.1 (26S proteasome regulatory subunit 8 homolog A-like [Benincasa hispida])

HSP 1 Score: 524.2 bits (1349), Expect = 7.3e-145
Identity = 298/413 (72.15%), Postives = 302/413 (73.12%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60
           MATMELDTKLPE   EE CSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR
Sbjct: 1   MATMELDTKLPETTIEETCSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60

Query: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK--------------------- 120
           NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK                     
Sbjct: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKIT 120

Query: 121 ------------------------------------------------------VIELPI 180
                                                                 VIELPI
Sbjct: 121 PSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 180

Query: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------- 240
           KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                         
Sbjct: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSR 240

Query: 241 -------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300
                  ++REHAPSIIFMDEIDSIGSARMESG+GNSDSEVQRTMLELLNQLDGFEASNK
Sbjct: 241 MVRELFVMAREHAPSIIFMDEIDSIGSARMESGTGNSDSEVQRTMLELLNQLDGFEASNK 300

Query: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 307
           IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA
Sbjct: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 360

BLAST of Pay0003985 vs. NCBI nr
Match: XP_022999363.1 (26S proteasome regulatory subunit 8 homolog A-like [Cucurbita maxima])

HSP 1 Score: 520.4 bits (1339), Expect = 1.1e-143
Identity = 297/413 (71.91%), Postives = 301/413 (72.88%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAKPATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60
           MATMELDT LPEA  EE CSAKPATK GEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR
Sbjct: 1   MATMELDTTLPEATLEETCSAKPATKQGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQR 60

Query: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK--------------------- 120
           NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK                     
Sbjct: 61  NELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKIT 120

Query: 121 ------------------------------------------------------VIELPI 180
                                                                 VIELPI
Sbjct: 121 PSARVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 180

Query: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------- 240
           KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                         
Sbjct: 181 KHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSR 240

Query: 241 -------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300
                  ++REH+PSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK
Sbjct: 241 MVRELFVMAREHSPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNK 300

Query: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 307
           IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA
Sbjct: 301 IKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIA 360

BLAST of Pay0003985 vs. TAIR 10
Match: AT5G19990.1 (regulatory particle triple-A ATPase 6A )

HSP 1 Score: 479.2 bits (1232), Expect = 2.5e-135
Identity = 277/414 (66.91%), Postives = 290/414 (70.05%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAK-PATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQ 60
           MA + +D++ PE   EE C+ K  A K GEGL+ YYLQHI +LQ QLR KT+NLNRLEAQ
Sbjct: 1   MAAVGVDSRRPETAMEETCNVKGAAAKQGEGLKQYYLQHIHELQRQLRQKTNNLNRLEAQ 60

Query: 61  RNELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK-------------------- 120
           RNELNSRVR+LREELQLLQEPGSYVGEVVKVMGKNKVLVK                    
Sbjct: 61  RNELNSRVRMLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKI 120

Query: 121 -------------------------------------------------------VIELP 180
                                                                  VIELP
Sbjct: 121 TPSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELP 180

Query: 181 IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------ 240
           IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                        
Sbjct: 181 IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGS 240

Query: 241 --------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASN 300
                   ++REHAPSIIFMDEIDSIGSARMESGSGN DSEVQRTMLELLNQLDGFEASN
Sbjct: 241 RMVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNGDSEVQRTMLELLNQLDGFEASN 300

Query: 301 KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI 307
           KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI
Sbjct: 301 KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI 360

BLAST of Pay0003985 vs. TAIR 10
Match: AT5G20000.1 (AAA-type ATPase family protein )

HSP 1 Score: 465.7 bits (1197), Expect = 2.9e-131
Identity = 271/414 (65.46%), Postives = 285/414 (68.84%), Query Frame = 0

Query: 1   MATMELDTKLPEAINEEACSAK-PATKHGEGLRHYYLQHIQDLQLQLRLKTHNLNRLEAQ 60
           MA + ++ + P    EE C+ K  A K GEGL  YYLQH+ +LQ   R K++NLNRLEAQ
Sbjct: 1   MAAVGVEARPPVTAMEETCNVKGAAAKQGEGLNKYYLQHLDELQRLQREKSYNLNRLEAQ 60

Query: 61  RNELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVK-------------------- 120
           RNELNSRVR+LREELQLLQEPGSYVGEVVKVMGKNKVLVK                    
Sbjct: 61  RNELNSRVRMLREELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVDIDKSIDITKL 120

Query: 121 -------------------------------------------------------VIELP 180
                                                                  VIELP
Sbjct: 121 TPSTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELP 180

Query: 181 IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVS------------------------ 240
           IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAV+                        
Sbjct: 181 IKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGS 240

Query: 241 --------LSREHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASN 300
                   ++REHAPSIIFMDEIDSIGSARMESGSGN DSEVQRTMLELLNQLDGFEASN
Sbjct: 241 RMVRELFVMAREHAPSIIFMDEIDSIGSARMESGSGNGDSEVQRTMLELLNQLDGFEASN 300

Query: 301 KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI 307
           KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI
Sbjct: 301 KIKVLMATNRIDILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKI 360

BLAST of Pay0003985 vs. TAIR 10
Match: AT5G43010.1 (regulatory particle triple-A ATPase 4A )

HSP 1 Score: 218.0 bits (554), Expect = 1.0e-56
Identity = 124/253 (49.01%), Postives = 161/253 (63.64%), Query Frame = 0

Query: 76  LLQEPGSYVGEVVKVMGKN-KVLVKVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTL 135
           L ++PG+     V  +G   + L + IELP+ +PELF  +GI  PKGVLLYGPPGTGKTL
Sbjct: 129 LHEDPGNISYSAVGGLGDQIRELRESIELPLMNPELFLRVGIKPPKGVLLYGPPGTGKTL 188

Query: 136 LARAV--------------------------------SLSREHAPSIIFMDEIDSIGSAR 195
           LARA+                                + +REH P IIFMDEID+IG  R
Sbjct: 189 LARAIASNIDANFLKVVSSAIIDKYIGESARLIREMFNYAREHQPCIIFMDEIDAIGGRR 248

Query: 196 MESGSGNSDSEVQRTMLELLNQLDGFEASNKIKVLMATNRIDILDQALLRPGRIDRKIEF 255
              G+ ++D E+QRT++ELLNQLDGF+   K+K++MATNR D+LD ALLRPGR+DRKIE 
Sbjct: 249 FSEGT-SADREIQRTLMELLNQLDGFDNLGKVKMIMATNRPDVLDPALLRPGRLDRKIEI 308

Query: 256 PNPNEESRFDILKIHSRKMNLMRGIDLKKIAEKMNGASGAELKAVCTEAGMFALRERRVH 296
           P PNE+SR DILKIH+  +     ID + I +   G +GA+L+ +CTEAGMFA+R  R +
Sbjct: 309 PLPNEQSRMDILKIHAAGIAKHGEIDYEAIVKLAEGFNGADLRNICTEAGMFAIRAERDY 368

BLAST of Pay0003985 vs. TAIR 10
Match: AT1G45000.1 (AAA-type ATPase family protein )

HSP 1 Score: 216.1 bits (549), Expect = 4.0e-56
Identity = 123/253 (48.62%), Postives = 161/253 (63.64%), Query Frame = 0

Query: 76  LLQEPGSYVGEVVKVMGKN-KVLVKVIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTL 135
           L ++PG+     V  +G   + L + IELP+ +PELF  +GI  PKGVLLYGPPGTGKTL
Sbjct: 129 LHEDPGNISYSAVGGLGDQIRELRESIELPLMNPELFLRVGIKPPKGVLLYGPPGTGKTL 188

Query: 136 LARAV--------------------------------SLSREHAPSIIFMDEIDSIGSAR 195
           LARA+                                + +REH P IIFMDEID+IG  R
Sbjct: 189 LARAIASNIDANFLKVVSSAIIDKYIGESARLIREMFNYAREHQPCIIFMDEIDAIGGRR 248

Query: 196 MESGSGNSDSEVQRTMLELLNQLDGFEASNKIKVLMATNRIDILDQALLRPGRIDRKIEF 255
              G+ ++D E+QRT++ELLNQLDGF+   K+K++MATNR D+LD ALLRPGR+DRKIE 
Sbjct: 249 FSEGT-SADREIQRTLMELLNQLDGFDQLGKVKMIMATNRPDVLDPALLRPGRLDRKIEI 308

Query: 256 PNPNEESRFDILKIHSRKMNLMRGIDLKKIAEKMNGASGAELKAVCTEAGMFALRERRVH 296
           P PNE+SR +ILKIH+  +     ID + I +   G +GA+L+ +CTEAGMFA+R  R +
Sbjct: 309 PLPNEQSRMEILKIHASGIAKHGEIDYEAIVKLGEGFNGADLRNICTEAGMFAIRAERDY 368

BLAST of Pay0003985 vs. TAIR 10
Match: AT3G05530.1 (regulatory particle triple-A ATPase 5A )

HSP 1 Score: 214.2 bits (544), Expect = 1.5e-55
Identity = 125/275 (45.45%), Postives = 169/275 (61.45%), Query Frame = 0

Query: 56  LEAQRNELNSRVRLLREELQLLQEPGSYVGEVVKVMGKNKVLVKVIELPIKHPELFESLG 115
           L+   +E +SRV+ +  +    ++P     ++  +  + + LV+ I LP+ H E FE LG
Sbjct: 146 LDTLPSEYDSRVKAMEVD----EKPTEDYNDIGGLEKQIQELVEAIVLPMTHKERFEKLG 205

Query: 116 IAQPKGVLLYGPPGTGKTLLARAVS--------------------------------LSR 175
           +  PKGVLLYGPPGTGKTL+ARA +                                L++
Sbjct: 206 VRPPKGVLLYGPPGTGKTLMARACAAQTNATFLKLAGPQLVQMFIGDGAKLVRDAFQLAK 265

Query: 176 EHAPSIIFMDEIDSIGSARMESGSGNSDSEVQRTMLELLNQLDGFEASNKIKVLMATNRI 235
           E AP IIF+DEID+IG+ R +S   + D EVQRTMLELLNQLDGF +  +IKV+ ATNR 
Sbjct: 266 EKAPCIIFIDEIDAIGTKRFDS-EVSGDREVQRTMLELLNQLDGFSSDERIKVIAATNRA 325

Query: 236 DILDQALLRPGRIDRKIEFPNPNEESRFDILKIHSRKMNLMRGIDLKKIAEKMNGASGAE 295
           DILD AL+R GR+DRKIEFP+P EE+R  IL+IHSRKMN+   ++ +++A   +  +GA+
Sbjct: 326 DILDPALMRSGRLDRKIEFPHPTEEARARILQIHSRKMNVHPDVNFEELARSTDDFNGAQ 385

Query: 296 LKAVCTEAGMFALRERRVHVTQEDFEMAVAKVMKK 299
           LKAVC EAGM ALR     V  EDF   + +V  K
Sbjct: 386 LKAVCVEAGMLALRRDATEVNHEDFNEGIIQVQAK 415

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C5U33.5e-13466.9126S proteasome regulatory subunit 8 homolog A OS=Arabidopsis thaliana OX=3702 GN... [more]
Q94BQ24.1e-13065.4626S proteasome regulatory subunit 8 homolog B OS=Arabidopsis thaliana OX=3702 GN... [more]
P621944.8e-10759.0226S proteasome regulatory subunit 8 OS=Bos taurus OX=9913 GN=PSMC5 PE=2 SV=1[more]
P621954.8e-10759.0226S proteasome regulatory subunit 8 OS=Homo sapiens OX=9606 GN=PSMC5 PE=1 SV=1[more]
P621964.8e-10759.0226S proteasome regulatory subunit 8 OS=Mus musculus OX=10090 GN=Psmc5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7V0824.5e-14873.3726S protease regulatory subunit 8-like protein A-like OS=Cucumis melo var. makuw... [more]
A0A1S3C7Z14.5e-14873.3726S protease regulatory subunit 8 homolog A-like OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A0A0LKW61.1e-14672.64AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G005970 PE=3 SV... [more]
A0A6J1KJI15.1e-14471.9126S proteasome regulatory subunit 8 homolog A-like OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1G4316.7e-14471.9126S proteasome regulatory subunit 8 homolog A-like OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
KAA0061582.19.2e-14873.3726S protease regulatory subunit 8-like protein A-like [Cucumis melo var. makuwa][more]
XP_008457948.19.2e-14873.37PREDICTED: 26S protease regulatory subunit 8 homolog A-like [Cucumis melo][more]
XP_004139327.12.3e-14672.6426S proteasome regulatory subunit 8 homolog A [Cucumis sativus] >KGN60651.1 hypo... [more]
XP_038891238.17.3e-14572.1526S proteasome regulatory subunit 8 homolog A-like [Benincasa hispida][more]
XP_022999363.11.1e-14371.9126S proteasome regulatory subunit 8 homolog A-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G19990.12.5e-13566.91regulatory particle triple-A ATPase 6A [more]
AT5G20000.12.9e-13165.46AAA-type ATPase family protein [more]
AT5G43010.11.0e-5649.01regulatory particle triple-A ATPase 4A [more]
AT1G45000.14.0e-5648.62AAA-type ATPase family protein [more]
AT3G05530.11.5e-5545.45regulatory particle triple-A ATPase 5A [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Payzawat) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 39..73
NoneNo IPR availableGENE3D2.40.50.140coord: 40..106
e-value: 7.1E-11
score: 44.0
NoneNo IPR availableGENE3D1.10.8.60coord: 229..305
e-value: 1.0E-30
score: 107.4
NoneNo IPR availablePANTHERPTHR2307326S PROTEASOME REGULATORY SUBUNITcoord: 20..100
NoneNo IPR availablePANTHERPTHR2307326S PROTEASOME REGULATORY SUBUNITcoord: 100..140
coord: 141..306
NoneNo IPR availableCDDcd00009AAAcoord: 109..223
e-value: 4.16439E-15
score: 69.4823
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 118..226
e-value: 2.9E-8
score: 43.4
IPR041569AAA ATPase, AAA+ lid domainPFAMPF17862AAA_lid_3coord: 247..289
e-value: 7.3E-15
score: 54.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 107..142
e-value: 3.9E-6
score: 28.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 143..225
e-value: 5.1E-27
score: 96.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 95..301
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 143..223
e-value: 8.9E-20
score: 71.5
coord: 122..139
e-value: 2.7E-5
score: 24.6
IPR03526126S proteasome regulatory subunit 8PANTHERPTHR23073:SF102BNAA02G04630D PROTEINcoord: 100..140
coord: 141..306
IPR03526126S proteasome regulatory subunit 8PANTHERPTHR23073:SF102BNAA02G04630D PROTEINcoord: 20..100
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 194..212

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Pay0003985.1Pay0003985.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
cellular_component GO:0008540 proteasome regulatory particle, base subcomplex
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0036402 proteasome-activating activity