PI0002674 (gene) Melon (PI 482460) v1

Overview
NamePI0002674
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionprotein PHYTOCHROME KINASE SUBSTRATE 1
Locationchr05: 11667593 .. 11669450 (-)
RNA-Seq ExpressionPI0002674
SyntenyPI0002674
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCATAACGTCTTGACAATCTCTCCCAACTTTTCTTCTTATAGAACCAAACTTTGTTCCTCATTTCTGAATGCTCAAATCCTATTATTTACCACTCTTTGATCAGCTCTCTATCTTCTCTTTGGTGTCGCCTTTTGTTTTGGGATCCAATTTTCCAATTCAATGGACATCTTCACCTCCATATCATCTAAAACATTGCAGTTTGATACCCATATTGATAGTAATAATAATTTGGGAGTTTACGGCGACACCTCCTTCTCGTCTTATTTGACTGCGAAAGAAGACCATAACTTCATCCGCAAACTCACTGAGTCTTGCCTAAAAAGCCCCAACATGATTCCAGGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCTGAAAAGTACTTCAATGGCGGAATGGAGGATGAGAGCACTCAAAAATCAAGCAACAATCATCCATCCTCTCAAAAGCTTGACAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAGTTGCCAAAGCCGAGGTTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGTCAAAGACCTTTGCTAAAGACGGTTGAAAGCACAACAACAACTGCAACAACAACGATCGCCAATAACAGTTATTCGCTGCAGAAGAGGAATAGTAACAACAACAAGAGCTTTCTATCCAACACTCTTGGCTATTGCATGTGTTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGCGAAATCAGTTTCAGCAATGCAGTTATAACAAACCCAACTCGAAACAATATTAATAATATATTGGATCGAGAAACGCCGTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTCCAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCATTAGAGGTTTTCGGTTCGCCTGTGACGGGAAGGCTCAGAAACAATAAACCAATCAGCCTGGAGAAAAGGCTAGCCATGCTTTCTTGGGATAACACAAATAACCACAACACCACGTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTCGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCATCACTGCCAGTGCACTCGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCGATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTTCCGAAGAGGCGACCAAGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGCAATAATAAGTATGAACGCAAAATGAGTGGTAATAAGTCCAACAACTTTGATTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTGATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTGGGAGGGTTAAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTCTTCCCCCGCCTCCGCCTCATCGTGCTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATTACCTTCAATATACAGTGAGATCATTGCATGTTATCTTCATATTTCCATAAGTAGATTAAACATTTGAACTATACTTATTTACTCTAATATAATAGGATATTTTCTTATTCACCACTATTCAAAATTTGAACATACCTTGGTACGAGTGGGGGGGTAAGTACATATTATTCTAAACTCTCCTGACTTTATTTGTAATTTTTTGTGAAAAGACTTTGAAAAATTCAATATGGATGTTCTTATAATTCGTTAGTA

mRNA sequence

CTTTCATAACGTCTTGACAATCTCTCCCAACTTTTCTTCTTATAGAACCAAACTTTGTTCCTCATTTCTGAATGCTCAAATCCTATTATTTACCACTCTTTGATCAGCTCTCTATCTTCTCTTTGGTGTCGCCTTTTGTTTTGGGATCCAATTTTCCAATTCAATGGACATCTTCACCTCCATATCATCTAAAACATTGCAGTTTGATACCCATATTGATAGTAATAATAATTTGGGAGTTTACGGCGACACCTCCTTCTCGTCTTATTTGACTGCGAAAGAAGACCATAACTTCATCCGCAAACTCACTGAGTCTTGCCTAAAAAGCCCCAACATGATTCCAGGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCTGAAAAGTACTTCAATGGCGGAATGGAGGATGAGAGCACTCAAAAATCAAGCAACAATCATCCATCCTCTCAAAAGCTTGACAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAGTTGCCAAAGCCGAGGTTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGTCAAAGACCTTTGCTAAAGACGGTTGAAAGCACAACAACAACTGCAACAACAACGATCGCCAATAACAGTTATTCGCTGCAGAAGAGGAATAGTAACAACAACAAGAGCTTTCTATCCAACACTCTTGGCTATTGCATGTGTTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGCGAAATCAGTTTCAGCAATGCAGTTATAACAAACCCAACTCGAAACAATATTAATAATATATTGGATCGAGAAACGCCGTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTCCAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCATTAGAGGTTTTCGGTTCGCCTGTGACGGGAAGGCTCAGAAACAATAAACCAATCAGCCTGGAGAAAAGGCTAGCCATGCTTTCTTGGGATAACACAAATAACCACAACACCACGTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTCGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCATCACTGCCAGTGCACTCGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCGATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTTCCGAAGAGGCGACCAAGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGCAATAATAAGTATGAACGCAAAATGAGTGGTAATAAGTCCAACAACTTTGATTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTGATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTGGGAGGGTTAAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTCTTCCCCCGCCTCCGCCTCATCGTGCTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATTACCTTCAATATACAGTGAGATCATTGCATGTTATCTTCATATTTCCATAAGTAGATTAAACATTTGAACTATACTTATTTACTCTAATATAATAGGATATTTTCTTATTCACCACTATTCAAAATTTGAACATACCTTGGTACGAGTGGGGGGGTAAGTACATATTATTCTAAACTCTCCTGACTTTATTTGTAATTTTTTGTGAAAAGACTTTGAAAAATTCAATATGGATGTTCTTATAATTCGTTAGTA

Coding sequence (CDS)

ATGGACATCTTCACCTCCATATCATCTAAAACATTGCAGTTTGATACCCATATTGATAGTAATAATAATTTGGGAGTTTACGGCGACACCTCCTTCTCGTCTTATTTGACTGCGAAAGAAGACCATAACTTCATCCGCAAACTCACTGAGTCTTGCCTAAAAAGCCCCAACATGATTCCAGGTAGCAGAGGCGGTGAAGATGGAGAGATTGGAATATTTGGAGCTGAAAAGTACTTCAATGGCGGAATGGAGGATGAGAGCACTCAAAAATCAAGCAACAATCATCCATCCTCTCAAAAGCTTGACAAACTTATTGTTGCTCATATGGAGGAAGCCTTGAAGTTGCCAAAGCCGAGGTTTGGAACTCCAAGTGTTGGATCGGAATCCAGTAGCGTCAACAGTCAAAGACCTTTGCTAAAGACGGTTGAAAGCACAACAACAACTGCAACAACAACGATCGCCAATAACAGTTATTCGCTGCAGAAGAGGAATAGTAACAACAACAAGAGCTTTCTATCCAACACTCTTGGCTATTGCATGTGTTGTACAAGCAACAAAAAAAGTACTGCAGTTGAAGACGTGGGCGAAATCAGTTTCAGCAATGCAGTTATAACAAACCCAACTCGAAACAATATTAATAATATATTGGATCGAGAAACGCCGTCGTTTCGAGGCTTTCCAACTGCAGCATCATCACTGAAGATGGTCCATTTCCAAGAACCTGAAGAAGTAGTTGAAAGAAAGTCATTAGAGGTTTTCGGTTCGCCTGTGACGGGAAGGCTCAGAAACAATAAACCAATCAGCCTGGAGAAAAGGCTAGCCATGCTTTCTTGGGATAACACAAATAACCACAACACCACGTTAGGATCAGGAATGTTTTACAACGAGGACGAGGTGAATAGCGATTGTAGCTCCGACTTGTTCGAGATTGAAAGCCTTACCAAGCAAACCAACCCATTCCACTCTCCAACTGCCTCTTGTTATGCGCCCAGTGAAGCTAGCGTTGACTGGAGCGTCATCACTGCCAGTGCACTCGACTTCGACGAACGGCGCCCCTCCACTACCAGTCCAGCCAGAGTGGTGCCACCACCACCGATGAGGGTTAATGTGCACAAAGAGGTTGTCGTTGTTCCGAAGAGGCGACCAAGTAGTATTTTAGGGTGTAAAAGTGAGAAAGCAGTAAGAGTTGCTGAAGGCAATAATAAGTATGAACGCAAAATGAGTGGTAATAAGTCCAACAACTTTGATTATCTTCTCATGGAACAACGTAACTCCGAGTCGTTGATGGCAGTTAAAAGGTTTGAAGATGAGACCAAAGTGGGAGGGTTAAGTTTCAAATCGCAAGGGTCGTCGTCGATGTTGCCTCTTCCCCCGCCTCCGCCTCATCGTGCTCTCGCCACACGCTCGCTTCCTCGCCCTTATTCCCCAAGGCTTACCAATATTACCTTCAATATACAGTGA

Protein sequence

MDIFTSISSKTLQFDTHIDSNNNLGVYGDTSFSSYLTAKEDHNFIRKLTESCLKSPNMIPGSRGGEDGEIGIFGAEKYFNGGMEDESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKPRFGTPSVGSESSSVNSQRPLLKTVESTTTTATTTIANNSYSLQKRNSNNNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAASSLKMVHFQEPEEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVITASALDFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMSGNKSNNFDYLLMEQRNSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLPPPPPHRALATRSLPRPYSPRLTNITFNIQ
Homology
BLAST of PI0002674 vs. ExPASy Swiss-Prot
Match: Q9SWI1 (Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Arabidopsis thaliana OX=3702 GN=PKS1 PE=1 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 8.6e-34
Identity = 152/438 (34.70%), Postives = 215/438 (49.09%), Query Frame = 0

Query: 5   TSISSKTLQFDTHIDSNNNLGVYGDTSFSSYLTAKEDHNFIRKLTESCLKSPNMIPG--- 64
           +S S+    FD   ++N++  +Y   S SSYL++KED     K      K+ NM      
Sbjct: 7   SSASTPKTSFDFMKNNNSHSSLY--VSSSSYLSSKEDALVTTKKLMEPSKTLNMSINPKQ 66

Query: 65  ----------SRGGEDGEIGIFGAEKYFNGGMEDESTQKSSNNHPSSQKLDKLIVAHMEE 124
                      +  ED EIG+FGAEKYFNG M  +S Q SS    ++ ++++ +V   + 
Sbjct: 67  EEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDM--DSDQGSSVLSLTNPEVERTVVDSKQS 126

Query: 125 ALKLPKPRFGTPSVGSESSSVNSQRPLLKTVESTTTTATTTIANNSYSLQKRNSNNNKSF 184
           A    K   GTPSV SE SS NSQ  LL+     +  ++     NS    ++ +NN KSF
Sbjct: 127 A----KKSTGTPSVRSE-SSWNSQSVLLQNKLVNSCNSSFKEKKNSNGQIQKVTNNKKSF 186

Query: 185 LSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAAS 244
           L+N LG C C  S+  S  VE+   +  S     +P   NI+ I  R         +A  
Sbjct: 187 LAN-LG-CKCACSDGDSVDVEEKTSVKRS----ADP---NISVITMRS--------SADM 246

Query: 245 SLKMVHFQEPEEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSG 304
           + +++  Q+ EE+ +RKSLEVFGSPV       K   ++K+L +  W +    + T   G
Sbjct: 247 NTELIKIQKQEELSQRKSLEVFGSPVA---IEKKSSVVQKKLPLPPWKSRTEEDDTKSEG 306

Query: 305 MFYNEDEVNSDCSSDLFEIESLTKQTNPF--------HSPTASCYAPSEASVDWSVITAS 364
                    SD SSDLFEIE LT    PF         SPT  CYAPSE SV+WS++TAS
Sbjct: 307 ---------SDSSSDLFEIEGLTGNPKPFLTRQGSDPASPT--CYAPSEVSVEWSIVTAS 366

Query: 365 ALDFDERRPSTTSPARVVPPPPM-RVNVHKEVVVVPKRRPSS--------ILGCKSEKAV 413
           A DF       TSP R   P  + R+ +  +    P+RR SS        ++ CKS K+V
Sbjct: 367 AADFSVMSECATSPVRRNRPTQIPRIPITAK--SAPQRRKSSSSSGGNGFLMSCKSHKSV 399

BLAST of PI0002674 vs. ExPASy Swiss-Prot
Match: Q9M9T4 (Protein PHYTOCHROME KINASE SUBSTRATE 2 OS=Arabidopsis thaliana OX=3702 GN=PKS2 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.6e-30
Identity = 151/431 (35.03%), Postives = 215/431 (49.88%), Query Frame = 0

Query: 5   TSISSKTLQFDTHIDSNNNL-GVYGDTSFS----SYLTAKEDHNFIRKLTESCLKS--PN 64
           +S S+  + FD  +++NNN   +YG  S S    SYLT+KED      LT+  L S   N
Sbjct: 6   SSSSTPNVSFDFMMNNNNNSNNLYGPFSSSSTSFSYLTSKED-----ALTQKNLMSGITN 65

Query: 65  MIPG--SRGGEDGEIGIFGAEKYFNGGMEDESTQKSSNNHPSSQ-KLDKLIVAHMEEALK 124
            + G   +  ED EI +FGAEKYFNG M+ + + +  +  P  +  ++++ V   + +  
Sbjct: 66  DVLGINKKASEDLEISVFGAEKYFNGDMDSDHSPRLVSPLPDPEVPIERIFVGPKQSS-- 125

Query: 125 LPKPRFGTPSVGSESSSVNSQRPLL--KTVESTTTTATTTIANNSYSLQKRNSN----NN 184
             K    TPS+ SE SS NSQ  LL  K VE        +  N+ +  +  +SN    N 
Sbjct: 126 --KNSSETPSLRSE-SSWNSQSLLLQSKYVEKKKNIKKNSSCNSYFQEKDMSSNHKVSNK 185

Query: 185 KSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPT 244
           KSFL+ TLG C C  SN  S  V D    S           + +  I  +   SF G   
Sbjct: 186 KSFLA-TLG-CRCVCSNWSSVDVVDDKRRS-----------SGLKKI--KTQLSFSG--- 245

Query: 245 AASSLKMVHFQEPEEVVE-RKSLEVFGSPVTGRLRNNKPISLEKRLAM--LSWD-NTNNH 304
             SS   +H Q+ E ++E RKSLE+FGSP+           +EKR+      W+ +++  
Sbjct: 246 DLSSEMKIHQQQQEAMLEQRKSLEIFGSPL-----------IEKRIIQKKFPWEYSSSAK 305

Query: 305 NTTLGSGMFYNEDEVN--SDCSSDLFEIESLTKQTNPFHSPTASC-------YAPSEASV 364
               G  + Y E+E    SD S+DLFEIESLT + NPF +   S        YAPSE S+
Sbjct: 306 KEEHGFSVKYEEEEDGSVSDVSTDLFEIESLTGKANPFLARQGSSDPDSPDGYAPSEVSI 365

Query: 365 DWSVITASALDFDERRPSTTSPAR-----VVPPPPMRVNVHKEVVVVPKRRPSS----IL 398
            WSV+TAS  DF       TSP +      +P  P+    ++E  + P+RR SS    ++
Sbjct: 366 QWSVVTASVADFSVMSECATSPVKKNRSFQIPRIPIMAKSNRE--IAPQRRKSSSSGLLM 395

BLAST of PI0002674 vs. ExPASy Swiss-Prot
Match: Q8GXS8 (Protein PHYTOCHROME KINASE SUBSTRATE 3 OS=Arabidopsis thaliana OX=3702 GN=PKS3 PE=2 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 7.1e-12
Identity = 115/422 (27.25%), Postives = 173/422 (41.00%), Query Frame = 0

Query: 63  RGGEDGEIGIFGAEKYFNGGMED-ESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKP--- 122
           +   D EIG+FGAEKYF+  ++  +ST   +  H      D             P P   
Sbjct: 41  KADNDAEIGVFGAEKYFSMKLDHVDSTADITKQHEKENTHDHPNANPNPNPNPHPHPHPQ 100

Query: 123 ------------RFGTPSVGSESSSVNSQRPLLKTVESTTTTATTTIANNSYSLQKRNSN 182
                       R GTPSV SE SS NSQ  L++            I N++ + Q++ ++
Sbjct: 101 LTKTTSSRSKTSRHGTPSVRSE-SSCNSQTFLMR------------INNHNENKQRKMND 160

Query: 183 NNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVITNP-----TRNNINNILDRETP 242
            + SF     G   C             G  S    V T+P     +RN+  + +  +  
Sbjct: 161 TSISF-----GGFRC------------YGPCSGVKTVHTDPKNSCKSRNSDRDFVAYDAR 220

Query: 243 SFRGFPTAASSLKMVHFQEPEEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWD-- 302
                P      K   F   E++    SL +         R++  ++LE++L++L+WD  
Sbjct: 221 KHNDKPRLHFEAKKADFHAQEKI----SLPI--------QRSDIAMNLERKLSLLTWDAI 280

Query: 303 -------NTNNHNTTLGSGMFYN--EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAP 362
                  N N++N    S M  N  E+E  S  SSDLFEIE++          T+S Y P
Sbjct: 281 PNQLSTKNNNHNNNGNNSSMSSNTQEEETASVASSDLFEIENI----------TSSVYEP 340

Query: 363 SEASVDWSVITASAL------DFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRRPSS 422
           SEAS+ WSV+T S        DFD  +  T +   V   P          V+  K R + 
Sbjct: 341 SEASIGWSVVTGSMADQSVISDFDMMKRVTRNGPVVKTKP----------VIGEKVRSAG 386

Query: 423 IL-GCKSEKAVRVAEGNNKYERKMSGNKSNNFDYLLMEQRNSESLMAVKRFEDETKVGGL 446
            L GCKS KAV V + + K +      ++   D+  M  +        K+F+ E ++  L
Sbjct: 401 FLSGCKSHKAVSVVDSSRKVK------ETAKVDHHEMSHQ--------KKFKTEIRIQDL 386

BLAST of PI0002674 vs. ExPASy TrEMBL
Match: A0A0A0LKV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108600 PE=4 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 5.5e-249
Identity = 451/490 (92.04%), Postives = 466/490 (95.10%), Query Frame = 0

Query: 1   MDIFTSISSKTLQFDTHIDSNNNLGVYGDTSFSSYLTAKEDHNFIRKLTESC--LKSPNM 60
           MDIFTSISSKTL FDTHIDSNN+LGVYGDTSFSSYL+AKEDH+FIRKL ES   LKSPNM
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60

Query: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IP SRGGEDGEIGIFGAEKYFN GMEDESTQ+SSNNHPSSQKLDKLIVAHMEEALKLPKP
Sbjct: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120

Query: 121 RFGTPSVGSESSSVNSQRPLLKTVESTTTTA--TTTIANNSYSLQKRNSNNNKSFLSNTL 180
           R GTPSVGSESSSVNSQRPLLK V+STTTT+   TTIA+NSYSLQKR+SNNNKSFLSNTL
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180

Query: 181 GYCMCCTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAASSLKMV 240
           GYCMCCTSNKKSTAVEDVGEISFSNA+ TNPTRNNINNILDRE PSFRGFPTAASSLKMV
Sbjct: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240

Query: 241 HFQEPEEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSGMFYNE 300
           H QEPEEVVERKSLEVFGSP+TGRLRNNKP+SLEKRL MLSWD TNNH+TTLGSGMFYNE
Sbjct: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300

Query: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVITASALDFDERRPSTTS 360
           DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSV+TASALDFDERRPSTTS
Sbjct: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360

Query: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMSGNKSNNFD 420
           PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKM GNKSNN D
Sbjct: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420

Query: 421 YLLMEQRNSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLPPPPPHRALATRSLPRPYSP 480
           YLLMEQRNSESLMAVKRFEDETKVGG SFKSQGSSSMLP+P P PH  LATRSLPRPYSP
Sbjct: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480

Query: 481 RLTNITFNIQ 487
           RLTNITFN+Q
Sbjct: 481 RLTNITFNMQ 490

BLAST of PI0002674 vs. ExPASy TrEMBL
Match: A0A5A7SNC2 (Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold745G00080 PE=4 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.1e-244
Identity = 450/491 (91.65%), Postives = 462/491 (94.09%), Query Frame = 0

Query: 1   MDIFTSISSKTLQFDTHIDSNNNLGVYGDTSFSSYLTAKEDHNFIRKLTESC--LKSPNM 60
           MDIFTSISSKTL FDTHIDSNNNLGVYGDTSFSSYLTAKEDH+FIRKLTES   LKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQ+S NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RFGTPSVGSESSSVNSQRPLLKTVESTTTTA--TTTIANNSYSLQK-RNSNNNKSFLSNT 180
           R GTPSVGSESSSVNSQRPLLK V+STTTT    TTIANNSYSLQK R+SNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNAV TNPTRNNINNILDRETPSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHFQEPEEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSGMFYN 300
           VH QEPEEVVERKSLEVFGSPVTGRLR+NKPISLEKRL MLSWD TNNH+TTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVITASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSV+TASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMSGNKSNNF 420
           SPARVVPPPPMRVNVHKEV VVPKRRPSSILGC SEKAVRVAEGNNKY+ KMSGNKSNN 
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLPPPPPHRALATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGGLSFKSQGSSSMLPL PP   RAL TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLAPPA--RALGTRSLPRPYS 480

Query: 481 PRLTNITFNIQ 487
           PRLTN+TFN+Q
Sbjct: 481 PRLTNMTFNMQ 489

BLAST of PI0002674 vs. ExPASy TrEMBL
Match: A0A1S3B4P0 (protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo OX=3656 GN=LOC103485779 PE=4 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.1e-244
Identity = 450/491 (91.65%), Postives = 462/491 (94.09%), Query Frame = 0

Query: 1   MDIFTSISSKTLQFDTHIDSNNNLGVYGDTSFSSYLTAKEDHNFIRKLTESC--LKSPNM 60
           MDIFTSISSKTL FDTHIDSNNNLGVYGDTSFSSYLTAKEDH+FIRKLTES   LKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQ+S NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RFGTPSVGSESSSVNSQRPLLKTVESTTTTA--TTTIANNSYSLQK-RNSNNNKSFLSNT 180
           R GTPSVGSESSSVNSQRPLLK V+STTTT    TTIANNSYSLQK R+SNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNAV TNPTRNNINNILDRETPSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHFQEPEEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSGMFYN 300
           VH QEPEEVVERKSLEVFGSPVTGRLR+NKPISLEKRL MLSWD TNNH+TTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVITASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSV+TASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMSGNKSNNF 420
           SPARVVPPPPMRVNVHKEV VVPKRRPSSILGC SEKAVRVAEGNNKY+ KMSGNKSNN 
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLPPPPPHRALATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGGLSFKSQGSSSMLPL PP   RAL TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLAPPA--RALGTRSLPRPYS 480

Query: 481 PRLTNITFNIQ 487
           PRLTN+TFN+Q
Sbjct: 481 PRLTNMTFNMQ 489

BLAST of PI0002674 vs. ExPASy TrEMBL
Match: A0A6J1K8F0 (protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493179 PE=4 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 1.4e-90
Identity = 232/428 (54.21%), Postives = 272/428 (63.55%), Query Frame = 0

Query: 68  GEIGIFGAEKYFNGGME---DESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKPRFGTPS 127
           GEIG+F AEKYFNGG++   D       NN P S+ LD        +    P P   TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRINPPNNKP-SKNLD-------PDGPFTPNPWLATPS 75

Query: 128 VGSES--SSVNSQRPLLKTVESTTTTATTTIANNSYSLQKRNSNNNKSFLSNTLG-YCMC 187
           V SES  ++ N+Q+PLL T                       ++N K FLS+ LG YCMC
Sbjct: 76  VASESCTTTTNTQKPLLNT---------------------HKTHNTKGFLSHALGYYCMC 135

Query: 188 CTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAASSLKMVHFQEP 247
            + + K T  +D+GEISFS  V T PT +  +N           F     SLK+VHFQEP
Sbjct: 136 YSRSDKPT--DDIGEISFSKPVTTAPTASFRDN----------SFTFQNGSLKLVHFQEP 195

Query: 248 EEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSGMFYNEDEVNS 307
           E  VERKSLEVFGSPV GR R NKP SLEK+LAMLSWD+TN+ +         N DE NS
Sbjct: 196 E--VERKSLEVFGSPVIGRHR-NKPFSLEKKLAMLSWDHTNSSSNN-------NNDEANS 255

Query: 308 DCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVITASALDFDERRPSTTSPARVV 367
           DCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSV+TASALDFDERR STTSPARV 
Sbjct: 256 DCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSPARVA 315

Query: 368 -PPPPMRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMSGNKSNNFDYLL 427
            PPPP RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   RK+SG  + N     
Sbjct: 316 PPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSN-GRKISGKVNFNSSSSS 375

Query: 428 MEQRNSESL-MAVKRFEDETKVGGLSFKSQGSSSMLPLPPPPPHRALATRSLPRPYSPRL 487
           +      S+ ++V R EDET+V   SF+SQ    +L    P  H ALATRSLPRPYSPRL
Sbjct: 376 VSVSVPVSVPVSVNRLEDETRVRSFSFRSQAPQPLL----PSSHHALATRSLPRPYSPRL 387

BLAST of PI0002674 vs. ExPASy TrEMBL
Match: A0A6J1GCF6 (protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452711 PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 3.7e-88
Identity = 229/427 (53.63%), Postives = 266/427 (62.30%), Query Frame = 0

Query: 68  GEIGIFGAEKYFNGGME---DESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKPRFGTPS 127
           GEIG+F AEKYFNGG++   D       NN PS        +    +    P P   TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRTNPPNNKPS--------INLAPDEPFTPNPWLATPS 75

Query: 128 VGSESSSVNSQRPLLKTVESTTTTATTTIANNSYSLQKRNSNNNKSFLSNTLG-YCMCCT 187
           V S               ES TTT TTT       L    ++N K FLS+ LG YCMC +
Sbjct: 76  VAS---------------ESCTTTTTTT---QKPLLNTHKTHNTKGFLSHALGYYCMCYS 135

Query: 188 SNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAASSLKMVHFQEPEE 247
            + K +  +D+GEISFS  V T PT +  +N           F     SLK+VHFQEPE 
Sbjct: 136 RSDKPS--DDIGEISFSRPVTTAPTASFRDN----------SFTFQNGSLKLVHFQEPE- 195

Query: 248 VVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSGMFYNEDEVNSDC 307
            VERKSLEVFGSPV GR R NKP SLEK+LAMLSWD+TNN+N        YN DE NSDC
Sbjct: 196 -VERKSLEVFGSPVIGRHR-NKPFSLEKKLAMLSWDHTNNNN--------YNNDEANSDC 255

Query: 308 SSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVITASALDFDERRPSTTSPARVV-- 367
           SSDLFEIESLTKQTNPF SPT S YAPSEASV+WSV+TASALDFDERR STTSPARV   
Sbjct: 256 SSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSPARVAAP 315

Query: 368 -PPPPMRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMSGNKS-NNFDYL 427
            PPPP RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   RK+SG  + N+    
Sbjct: 316 PPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSN-GRKISGKVNFNSSSSS 375

Query: 428 LMEQRNSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLPPPPPHRALATRSLPRPYSPRL 486
           +         ++V RFEDET+V   SF+SQ    +L    P    +LATRSLPRPYSPRL
Sbjct: 376 VSVSVPVPVSVSVNRFEDETRVRSFSFRSQAPPPLL----PSSRHSLATRSLPRPYSPRL 388

BLAST of PI0002674 vs. NCBI nr
Match: XP_004138989.1 (protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis sativus] >KGN61382.1 hypothetical protein Csa_006559 [Cucumis sativus])

HSP 1 Score: 869.8 bits (2246), Expect = 1.1e-248
Identity = 451/490 (92.04%), Postives = 466/490 (95.10%), Query Frame = 0

Query: 1   MDIFTSISSKTLQFDTHIDSNNNLGVYGDTSFSSYLTAKEDHNFIRKLTESC--LKSPNM 60
           MDIFTSISSKTL FDTHIDSNN+LGVYGDTSFSSYL+AKEDH+FIRKL ES   LKSPNM
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNSLGVYGDTSFSSYLSAKEDHDFIRKLAESTRYLKSPNM 60

Query: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IP SRGGEDGEIGIFGAEKYFN GMEDESTQ+SSNNHPSSQKLDKLIVAHMEEALKLPKP
Sbjct: 61  IPCSRGGEDGEIGIFGAEKYFNDGMEDESTQRSSNNHPSSQKLDKLIVAHMEEALKLPKP 120

Query: 121 RFGTPSVGSESSSVNSQRPLLKTVESTTTTA--TTTIANNSYSLQKRNSNNNKSFLSNTL 180
           R GTPSVGSESSSVNSQRPLLK V+STTTT+   TTIA+NSYSLQKR+SNNNKSFLSNTL
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTSAIATTIASNSYSLQKRSSNNNKSFLSNTL 180

Query: 181 GYCMCCTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAASSLKMV 240
           GYCMCCTSNKKSTAVEDVGEISFSNA+ TNPTRNNINNILDRE PSFRGFPTAASSLKMV
Sbjct: 181 GYCMCCTSNKKSTAVEDVGEISFSNAITTNPTRNNINNILDREAPSFRGFPTAASSLKMV 240

Query: 241 HFQEPEEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSGMFYNE 300
           H QEPEEVVERKSLEVFGSP+TGRLRNNKP+SLEKRL MLSWD TNNH+TTLGSGMFYNE
Sbjct: 241 HLQEPEEVVERKSLEVFGSPMTGRLRNNKPMSLEKRLTMLSWDKTNNHSTTLGSGMFYNE 300

Query: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVITASALDFDERRPSTTS 360
           DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSV+TASALDFDERRPSTTS
Sbjct: 301 DEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSTTS 360

Query: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMSGNKSNNFD 420
           PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKM GNKSNN D
Sbjct: 361 PARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMGGNKSNNSD 420

Query: 421 YLLMEQRNSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLPPPPPHRALATRSLPRPYSP 480
           YLLMEQRNSESLMAVKRFEDETKVGG SFKSQGSSSMLP+P P PH  LATRSLPRPYSP
Sbjct: 421 YLLMEQRNSESLMAVKRFEDETKVGGFSFKSQGSSSMLPIPLPLPHHGLATRSLPRPYSP 480

Query: 481 RLTNITFNIQ 487
           RLTNITFN+Q
Sbjct: 481 RLTNITFNMQ 490

BLAST of PI0002674 vs. NCBI nr
Match: XP_008441702.1 (PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo] >KAA0032290.1 protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo var. makuwa] >TYK13512.1 protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo var. makuwa])

HSP 1 Score: 855.5 bits (2209), Expect = 2.2e-244
Identity = 450/491 (91.65%), Postives = 462/491 (94.09%), Query Frame = 0

Query: 1   MDIFTSISSKTLQFDTHIDSNNNLGVYGDTSFSSYLTAKEDHNFIRKLTESC--LKSPNM 60
           MDIFTSISSKTL FDTHIDSNNNLGVYGDTSFSSYLTAKEDH+FIRKLTES   LKSP M
Sbjct: 1   MDIFTSISSKTLPFDTHIDSNNNLGVYGDTSFSSYLTAKEDHDFIRKLTESTRYLKSPIM 60

Query: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKP 120
           IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQ+S NNHPSSQK DKLIVAHMEEALKLPKP
Sbjct: 61  IPGSRGGEDGEIGIFGAEKYFNGGMEDESTQRSGNNHPSSQKFDKLIVAHMEEALKLPKP 120

Query: 121 RFGTPSVGSESSSVNSQRPLLKTVESTTTTA--TTTIANNSYSLQK-RNSNNNKSFLSNT 180
           R GTPSVGSESSSVNSQRPLLK V+STTTT    TTIANNSYSLQK R+SNNNKSFLSNT
Sbjct: 121 RLGTPSVGSESSSVNSQRPLLKIVKSTTTTTAIATTIANNSYSLQKRRSSNNNKSFLSNT 180

Query: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAASSLKM 240
           LGYCMCCTSNKKSTAVEDVGEISFSNAV TNPTRNNINNILDRETPSFRGFPTAASS+KM
Sbjct: 181 LGYCMCCTSNKKSTAVEDVGEISFSNAVTTNPTRNNINNILDRETPSFRGFPTAASSMKM 240

Query: 241 VHFQEPEEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSGMFYN 300
           VH QEPEEVVERKSLEVFGSPVTGRLR+NKPISLEKRL MLSWD TNNH+TTLGSGMFYN
Sbjct: 241 VHLQEPEEVVERKSLEVFGSPVTGRLRSNKPISLEKRLTMLSWDKTNNHSTTLGSGMFYN 300

Query: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVITASALDFDERRPSTT 360
           EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSV+TASALDFDERRPS T
Sbjct: 301 EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVVTASALDFDERRPSIT 360

Query: 361 SPARVVPPPPMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMSGNKSNNF 420
           SPARVVPPPPMRVNVHKEV VVPKRRPSSILGC SEKAVRVAEGNNKY+ KMSGNKSNN 
Sbjct: 361 SPARVVPPPPMRVNVHKEVFVVPKRRPSSILGCNSEKAVRVAEGNNKYDHKMSGNKSNNS 420

Query: 421 DYLLMEQRNSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLPPPPPHRALATRSLPRPYS 480
           DYL MEQR+SESLMAVKRFEDETKVGGLSFKSQGSSSMLPL PP   RAL TRSLPRPYS
Sbjct: 421 DYLHMEQRDSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLAPPA--RALGTRSLPRPYS 480

Query: 481 PRLTNITFNIQ 487
           PRLTN+TFN+Q
Sbjct: 481 PRLTNMTFNMQ 489

BLAST of PI0002674 vs. NCBI nr
Match: XP_038889450.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Benincasa hispida])

HSP 1 Score: 669.1 bits (1725), Expect = 2.9e-188
Identity = 391/508 (76.97%), Postives = 426/508 (83.86%), Query Frame = 0

Query: 1   MDIFTSISSKTLQFDTHID-SNNNLGVYGDTSFSSYLTAKEDHNF-IRKLTESC--LKSP 60
           MDIF+SIS+KTL FDTHID +NNN+GVYGD   SSY   KEDH F I KLTES   LKS 
Sbjct: 1   MDIFSSISTKTLPFDTHIDNNNNNIGVYGDP--SSY---KEDHTFIIHKLTESTRYLKSS 60

Query: 61  NMIPGSRGGEDGEIGIFGAEKYFNGGMEDESTQKSSNNHPSSQKLDKLIV--AH-----M 120
           N IPGS G EDGEIGIFGAEKYFNGG+E++ T+       S+QKLDKL+V  AH     +
Sbjct: 61  NTIPGSGGHEDGEIGIFGAEKYFNGGIENDGTR----TERSAQKLDKLLVLAAHQQHNLL 120

Query: 121 EEALKLPKPRFGTPSVGSESSSVNSQRPLLKTVESTTTTATTTIANNSYSLQKR--NSNN 180
           EE+LKLPKPR GTPSVGSESSS+NSQRPLL  V++     +TTIANN+YSLQKR  +SN+
Sbjct: 121 EESLKLPKPRLGTPSVGSESSSINSQRPLLNVVKN-----STTIANNNYSLQKRSSSSNH 180

Query: 181 NKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVITNPTR-----NNINNILDRETPS 240
           NKSFLSNT GYCMCC+S++KS AVEDVGEISFSNAV T PTR     NN NNI +RETPS
Sbjct: 181 NKSFLSNTFGYCMCCSSSEKS-AVEDVGEISFSNAVTTKPTRSNNNNNNNNNIFERETPS 240

Query: 241 FRGFPTAA--SSLKMVHFQEPEEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDN 300
           FRGFPTAA  SSLKM+HFQEPEEV ERKSLEVFGSPV GRLRNNKPISLEKRLAMLSWD+
Sbjct: 241 FRGFPTAASSSSLKMIHFQEPEEVGERKSLEVFGSPVMGRLRNNKPISLEKRLAMLSWDH 300

Query: 301 TNNHNTTLGSGMFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVI 360
           TNN+N +LGSG+FYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSV+
Sbjct: 301 TNNNNNSLGSGVFYNEDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVV 360

Query: 361 TASALDFDERRPSTTSPARVVPPP-PMRVNVHKEVVVVPKRRPSSILGCKSEKAVRVAEG 420
           TASALDFDERR STTSP RVV P  PMRVNV+KE  VV KRRPSSILGCKSEKAVRVAE 
Sbjct: 361 TASALDFDERRLSTTSPTRVVAPSLPMRVNVNKE--VVQKRRPSSILGCKSEKAVRVAED 420

Query: 421 NNKYERKMSGNKSNNFDYLLMEQRNSESLMAVKRFEDETKVGGLSFKSQGSS-SMLPLPP 480
           NNKY RKM+G KSN  DY      NSESL+AVKR EDETKVGGLSF+SQGSS S+LPL P
Sbjct: 421 NNKYGRKMNG-KSNYCDY------NSESLVAVKRSEDETKVGGLSFRSQGSSASLLPL-P 480

Query: 481 PPPHRALATRSLPRPYSPRLTNITFNIQ 487
           PPPHRALATR LPRPYSPRLTNI+FNIQ
Sbjct: 481 PPPHRALATRPLPRPYSPRLTNISFNIQ 483

BLAST of PI0002674 vs. NCBI nr
Match: XP_022998592.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita maxima])

HSP 1 Score: 343.6 bits (880), Expect = 2.8e-90
Identity = 232/428 (54.21%), Postives = 272/428 (63.55%), Query Frame = 0

Query: 68  GEIGIFGAEKYFNGGME---DESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKPRFGTPS 127
           GEIG+F AEKYFNGG++   D       NN P S+ LD        +    P P   TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRINPPNNKP-SKNLD-------PDGPFTPNPWLATPS 75

Query: 128 VGSES--SSVNSQRPLLKTVESTTTTATTTIANNSYSLQKRNSNNNKSFLSNTLG-YCMC 187
           V SES  ++ N+Q+PLL T                       ++N K FLS+ LG YCMC
Sbjct: 76  VASESCTTTTNTQKPLLNT---------------------HKTHNTKGFLSHALGYYCMC 135

Query: 188 CTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAASSLKMVHFQEP 247
            + + K T  +D+GEISFS  V T PT +  +N           F     SLK+VHFQEP
Sbjct: 136 YSRSDKPT--DDIGEISFSKPVTTAPTASFRDN----------SFTFQNGSLKLVHFQEP 195

Query: 248 EEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSGMFYNEDEVNS 307
           E  VERKSLEVFGSPV GR R NKP SLEK+LAMLSWD+TN+ +         N DE NS
Sbjct: 196 E--VERKSLEVFGSPVIGRHR-NKPFSLEKKLAMLSWDHTNSSSNN-------NNDEANS 255

Query: 308 DCSSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVITASALDFDERRPSTTSPARVV 367
           DCSSDLFEIESLTKQTNPF SPT S YAPSEASV+WSV+TASALDFDERR STTSPARV 
Sbjct: 256 DCSSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSPARVA 315

Query: 368 -PPPPMRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMSGNKSNNFDYLL 427
            PPPP RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   RK+SG  + N     
Sbjct: 316 PPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSN-GRKISGKVNFNSSSSS 375

Query: 428 MEQRNSESL-MAVKRFEDETKVGGLSFKSQGSSSMLPLPPPPPHRALATRSLPRPYSPRL 487
           +      S+ ++V R EDET+V   SF+SQ    +L    P  H ALATRSLPRPYSPRL
Sbjct: 376 VSVSVPVSVPVSVNRLEDETRVRSFSFRSQAPQPLL----PSSHHALATRSLPRPYSPRL 387

BLAST of PI0002674 vs. NCBI nr
Match: XP_022949325.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita moschata])

HSP 1 Score: 335.5 bits (859), Expect = 7.7e-88
Identity = 229/427 (53.63%), Postives = 266/427 (62.30%), Query Frame = 0

Query: 68  GEIGIFGAEKYFNGGME---DESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKPRFGTPS 127
           GEIG+F AEKYFNGG++   D       NN PS        +    +    P P   TPS
Sbjct: 16  GEIGVFRAEKYFNGGLDIHVDSPRTNPPNNKPS--------INLAPDEPFTPNPWLATPS 75

Query: 128 VGSESSSVNSQRPLLKTVESTTTTATTTIANNSYSLQKRNSNNNKSFLSNTLG-YCMCCT 187
           V S               ES TTT TTT       L    ++N K FLS+ LG YCMC +
Sbjct: 76  VAS---------------ESCTTTTTTT---QKPLLNTHKTHNTKGFLSHALGYYCMCYS 135

Query: 188 SNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAASSLKMVHFQEPEE 247
            + K +  +D+GEISFS  V T PT +  +N           F     SLK+VHFQEPE 
Sbjct: 136 RSDKPS--DDIGEISFSRPVTTAPTASFRDN----------SFTFQNGSLKLVHFQEPE- 195

Query: 248 VVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSGMFYNEDEVNSDC 307
            VERKSLEVFGSPV GR R NKP SLEK+LAMLSWD+TNN+N        YN DE NSDC
Sbjct: 196 -VERKSLEVFGSPVIGRHR-NKPFSLEKKLAMLSWDHTNNNN--------YNNDEANSDC 255

Query: 308 SSDLFEIESLTKQTNPFHSPTASCYAPSEASVDWSVITASALDFDERRPSTTSPARVV-- 367
           SSDLFEIESLTKQTNPF SPT S YAPSEASV+WSV+TASALDFDERR STTSPARV   
Sbjct: 256 SSDLFEIESLTKQTNPFQSPTPSYYAPSEASVEWSVVTASALDFDERRISTTSPARVAAP 315

Query: 368 -PPPPMRVNVHKEV-VVVPKRRPSSILGCKSEKAVRVAEGNNKYERKMSGNKS-NNFDYL 427
            PPPP RV V+++V VVV KRR  S+LGCKSEKAVRVAE N+   RK+SG  + N+    
Sbjct: 316 PPPPPRRVGVNEQVAVVVQKRRSGSLLGCKSEKAVRVAEDNSN-GRKISGKVNFNSSSSS 375

Query: 428 LMEQRNSESLMAVKRFEDETKVGGLSFKSQGSSSMLPLPPPPPHRALATRSLPRPYSPRL 486
           +         ++V RFEDET+V   SF+SQ    +L    P    +LATRSLPRPYSPRL
Sbjct: 376 VSVSVPVPVSVSVNRFEDETRVRSFSFRSQAPPPLL----PSSRHSLATRSLPRPYSPRL 388

BLAST of PI0002674 vs. TAIR 10
Match: AT2G02950.1 (phytochrome kinase substrate 1 )

HSP 1 Score: 146.4 bits (368), Expect = 6.1e-35
Identity = 152/438 (34.70%), Postives = 215/438 (49.09%), Query Frame = 0

Query: 5   TSISSKTLQFDTHIDSNNNLGVYGDTSFSSYLTAKEDHNFIRKLTESCLKSPNMIPG--- 64
           +S S+    FD   ++N++  +Y   S SSYL++KED     K      K+ NM      
Sbjct: 7   SSASTPKTSFDFMKNNNSHSSLY--VSSSSYLSSKEDALVTTKKLMEPSKTLNMSINPKQ 66

Query: 65  ----------SRGGEDGEIGIFGAEKYFNGGMEDESTQKSSNNHPSSQKLDKLIVAHMEE 124
                      +  ED EIG+FGAEKYFNG M  +S Q SS    ++ ++++ +V   + 
Sbjct: 67  EEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDM--DSDQGSSVLSLTNPEVERTVVDSKQS 126

Query: 125 ALKLPKPRFGTPSVGSESSSVNSQRPLLKTVESTTTTATTTIANNSYSLQKRNSNNNKSF 184
           A    K   GTPSV SE SS NSQ  LL+     +  ++     NS    ++ +NN KSF
Sbjct: 127 A----KKSTGTPSVRSE-SSWNSQSVLLQNKLVNSCNSSFKEKKNSNGQIQKVTNNKKSF 186

Query: 185 LSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPTAAS 244
           L+N LG C C  S+  S  VE+   +  S     +P   NI+ I  R         +A  
Sbjct: 187 LAN-LG-CKCACSDGDSVDVEEKTSVKRS----ADP---NISVITMRS--------SADM 246

Query: 245 SLKMVHFQEPEEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWDNTNNHNTTLGSG 304
           + +++  Q+ EE+ +RKSLEVFGSPV       K   ++K+L +  W +    + T   G
Sbjct: 247 NTELIKIQKQEELSQRKSLEVFGSPVA---IEKKSSVVQKKLPLPPWKSRTEEDDTKSEG 306

Query: 305 MFYNEDEVNSDCSSDLFEIESLTKQTNPF--------HSPTASCYAPSEASVDWSVITAS 364
                    SD SSDLFEIE LT    PF         SPT  CYAPSE SV+WS++TAS
Sbjct: 307 ---------SDSSSDLFEIEGLTGNPKPFLTRQGSDPASPT--CYAPSEVSVEWSIVTAS 366

Query: 365 ALDFDERRPSTTSPARVVPPPPM-RVNVHKEVVVVPKRRPSS--------ILGCKSEKAV 413
           A DF       TSP R   P  + R+ +  +    P+RR SS        ++ CKS K+V
Sbjct: 367 AADFSVMSECATSPVRRNRPTQIPRIPITAK--SAPQRRKSSSSSGGNGFLMSCKSHKSV 399

BLAST of PI0002674 vs. TAIR 10
Match: AT1G14280.1 (phytochrome kinase substrate 2 )

HSP 1 Score: 134.8 bits (338), Expect = 1.8e-31
Identity = 151/431 (35.03%), Postives = 215/431 (49.88%), Query Frame = 0

Query: 5   TSISSKTLQFDTHIDSNNNL-GVYGDTSFS----SYLTAKEDHNFIRKLTESCLKS--PN 64
           +S S+  + FD  +++NNN   +YG  S S    SYLT+KED      LT+  L S   N
Sbjct: 6   SSSSTPNVSFDFMMNNNNNSNNLYGPFSSSSTSFSYLTSKED-----ALTQKNLMSGITN 65

Query: 65  MIPG--SRGGEDGEIGIFGAEKYFNGGMEDESTQKSSNNHPSSQ-KLDKLIVAHMEEALK 124
            + G   +  ED EI +FGAEKYFNG M+ + + +  +  P  +  ++++ V   + +  
Sbjct: 66  DVLGINKKASEDLEISVFGAEKYFNGDMDSDHSPRLVSPLPDPEVPIERIFVGPKQSS-- 125

Query: 125 LPKPRFGTPSVGSESSSVNSQRPLL--KTVESTTTTATTTIANNSYSLQKRNSN----NN 184
             K    TPS+ SE SS NSQ  LL  K VE        +  N+ +  +  +SN    N 
Sbjct: 126 --KNSSETPSLRSE-SSWNSQSLLLQSKYVEKKKNIKKNSSCNSYFQEKDMSSNHKVSNK 185

Query: 185 KSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVITNPTRNNINNILDRETPSFRGFPT 244
           KSFL+ TLG C C  SN  S  V D    S           + +  I  +   SF G   
Sbjct: 186 KSFLA-TLG-CRCVCSNWSSVDVVDDKRRS-----------SGLKKI--KTQLSFSG--- 245

Query: 245 AASSLKMVHFQEPEEVVE-RKSLEVFGSPVTGRLRNNKPISLEKRLAM--LSWD-NTNNH 304
             SS   +H Q+ E ++E RKSLE+FGSP+           +EKR+      W+ +++  
Sbjct: 246 DLSSEMKIHQQQQEAMLEQRKSLEIFGSPL-----------IEKRIIQKKFPWEYSSSAK 305

Query: 305 NTTLGSGMFYNEDEVN--SDCSSDLFEIESLTKQTNPFHSPTASC-------YAPSEASV 364
               G  + Y E+E    SD S+DLFEIESLT + NPF +   S        YAPSE S+
Sbjct: 306 KEEHGFSVKYEEEEDGSVSDVSTDLFEIESLTGKANPFLARQGSSDPDSPDGYAPSEVSI 365

Query: 365 DWSVITASALDFDERRPSTTSPAR-----VVPPPPMRVNVHKEVVVVPKRRPSS----IL 398
            WSV+TAS  DF       TSP +      +P  P+    ++E  + P+RR SS    ++
Sbjct: 366 QWSVVTASVADFSVMSECATSPVKKNRSFQIPRIPIMAKSNRE--IAPQRRKSSSSGLLM 395

BLAST of PI0002674 vs. TAIR 10
Match: AT1G18810.1 (phytochrome kinase substrate-related )

HSP 1 Score: 73.6 bits (179), Expect = 5.1e-13
Identity = 115/422 (27.25%), Postives = 173/422 (41.00%), Query Frame = 0

Query: 63  RGGEDGEIGIFGAEKYFNGGMED-ESTQKSSNNHPSSQKLDKLIVAHMEEALKLPKP--- 122
           +   D EIG+FGAEKYF+  ++  +ST   +  H      D             P P   
Sbjct: 41  KADNDAEIGVFGAEKYFSMKLDHVDSTADITKQHEKENTHDHPNANPNPNPNPHPHPHPQ 100

Query: 123 ------------RFGTPSVGSESSSVNSQRPLLKTVESTTTTATTTIANNSYSLQKRNSN 182
                       R GTPSV SE SS NSQ  L++            I N++ + Q++ ++
Sbjct: 101 LTKTTSSRSKTSRHGTPSVRSE-SSCNSQTFLMR------------INNHNENKQRKMND 160

Query: 183 NNKSFLSNTLGYCMCCTSNKKSTAVEDVGEISFSNAVITNP-----TRNNINNILDRETP 242
            + SF     G   C             G  S    V T+P     +RN+  + +  +  
Sbjct: 161 TSISF-----GGFRC------------YGPCSGVKTVHTDPKNSCKSRNSDRDFVAYDAR 220

Query: 243 SFRGFPTAASSLKMVHFQEPEEVVERKSLEVFGSPVTGRLRNNKPISLEKRLAMLSWD-- 302
                P      K   F   E++    SL +         R++  ++LE++L++L+WD  
Sbjct: 221 KHNDKPRLHFEAKKADFHAQEKI----SLPI--------QRSDIAMNLERKLSLLTWDAI 280

Query: 303 -------NTNNHNTTLGSGMFYN--EDEVNSDCSSDLFEIESLTKQTNPFHSPTASCYAP 362
                  N N++N    S M  N  E+E  S  SSDLFEIE++          T+S Y P
Sbjct: 281 PNQLSTKNNNHNNNGNNSSMSSNTQEEETASVASSDLFEIENI----------TSSVYEP 340

Query: 363 SEASVDWSVITASAL------DFDERRPSTTSPARVVPPPPMRVNVHKEVVVVPKRRPSS 422
           SEAS+ WSV+T S        DFD  +  T +   V   P          V+  K R + 
Sbjct: 341 SEASIGWSVVTGSMADQSVISDFDMMKRVTRNGPVVKTKP----------VIGEKVRSAG 386

Query: 423 IL-GCKSEKAVRVAEGNNKYERKMSGNKSNNFDYLLMEQRNSESLMAVKRFEDETKVGGL 446
            L GCKS KAV V + + K +      ++   D+  M  +        K+F+ E ++  L
Sbjct: 401 FLSGCKSHKAVSVVDSSRKVK------ETAKVDHHEMSHQ--------KKFKTEIRIQDL 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SWI18.6e-3434.70Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Arabidopsis thaliana OX=3702 GN=PKS1 P... [more]
Q9M9T42.6e-3035.03Protein PHYTOCHROME KINASE SUBSTRATE 2 OS=Arabidopsis thaliana OX=3702 GN=PKS2 P... [more]
Q8GXS87.1e-1227.25Protein PHYTOCHROME KINASE SUBSTRATE 3 OS=Arabidopsis thaliana OX=3702 GN=PKS3 P... [more]
Match NameE-valueIdentityDescription
A0A0A0LKV25.5e-24992.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G108600 PE=4 SV=1[more]
A0A5A7SNC21.1e-24491.65Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B4P01.1e-24491.65protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Cucumis melo OX=3656 GN=LOC103485779 P... [more]
A0A6J1K8F01.4e-9054.21protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1GCF63.7e-8853.63protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_004138989.11.1e-24892.04protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis sativus] >KGN61382.1 hypothetica... [more]
XP_008441702.12.2e-24491.65PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis melo] >KAA0032290.1 p... [more]
XP_038889450.12.9e-18876.97protein PHYTOCHROME KINASE SUBSTRATE 1-like [Benincasa hispida][more]
XP_022998592.12.8e-9054.21protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita maxima][more]
XP_022949325.17.7e-8853.63protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G02950.16.1e-3534.70phytochrome kinase substrate 1 [more]
AT1G14280.11.8e-3135.03phytochrome kinase substrate 2 [more]
AT1G18810.15.1e-1327.25phytochrome kinase substrate-related [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 123..138
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 117..138
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 442..473
NoneNo IPR availablePANTHERPTHR33781:SF4PROTEIN PHYTOCHROME KINASE SUBSTRATE 1coord: 3..442
IPR039615Protein PHYTOCHROME KINASE SUBSTRATEPANTHERPTHR33781PROTEIN PHYTOCHROME KINASE SUBSTRATE 1-RELATEDcoord: 3..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0002674.1PI0002674.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0009638 phototropism
molecular_function GO:0016301 kinase activity