PI0028466 (gene) Melon (PI 482460) v1

Overview


Name	PI0028466
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Retrovirus-related Pol polyprotein
Location	chr03: 15829315 .. 15829617 (-)
RNA-Seq Expression	PI0028466
Synteny	PI0028466

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTATCACATGGCATAGTTTTAGGTCATTTAGTATCCTCAAAAGAAATAGAAGTAGATAAAGCAAAGATTGATGTTATTCAAAAATTGCCATATCCTACTTGTCTAAAAGATGTTAGATCTTTCCTTGGTAGTGTCGGCTTTTATAGAAGGTTTATAAAGGATTTTTCTAAAATTGCTTTACCTCTAACTTCTCTCTTGCAAAAAGATGTGCCATTTGTGATTGATGACAAATGTAAAAAGGCATTCAATGAACTCAAGCAAAGGTTCTTCTCTACTCCTATCCTTCAATCTCCTAATTAG

mRNA sequence

ATGGTATCACATGATAAAGCAAAGATTGATGTTATTCAAAAATTGCCATATCCTACTTGTCTAAAAGATGTTAGATCTTTCCTTGGTAGTGTCGGCTTTTATAGAAGGTTTATAAAGGATTTTTCTAAAATTGCTTTACCTCTAACTTCTCTCTTGCAAAAAGATGTGCCATTTGTGATTGATGACAAATGTAAAAAGGCATTCAATGAACTCAAGCAAAGGTTCTTCTCTACTCCTATCCTTCAATCTCCTAATTAG

Coding sequence (CDS)

ATGGTATCACATGATAAAGCAAAGATTGATGTTATTCAAAAATTGCCATATCCTACTTGTCTAAAAGATGTTAGATCTTTCCTTGGTAGTGTCGGCTTTTATAGAAGGTTTATAAAGGATTTTTCTAAAATTGCTTTACCTCTAACTTCTCTCTTGCAAAAAGATGTGCCATTTGTGATTGATGACAAATGTAAAAAGGCATTCAATGAACTCAAGCAAAGGTTCTTCTCTACTCCTATCCTTCAATCTCCTAATTAG

Protein sequence

MVSHDKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKCKKAFNELKQRFFSTPILQSPN

Homology

[Close]

BLAST of PI0028466 vs. ExPASy Swiss-Prot
Match: P10394 (Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaster OX=7227 GN=POL PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 4.0e-11
Identity = 33/81 (40.74%), Postives = 47/81 (58.02%), Query Frame = 0


Query: 5   DKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKC 64
           D  K DVIQ  P P      R F+    +YRRFIK+F+  +  +T L +K+VPF   D+C
Sbjct: 531 DDKKYDVIQNYPVPHDADSARRFVAFCNYYRRFIKNFADYSRHITRLCKKNVPFEWTDEC 590

Query: 65  KKAFNELKQRFFSTPILQSPN 86
           +KAF  LK +  +  +LQ P+
Sbjct: 591 QKAFIHLKSQLINPTLLQYPD 611

[Close]

BLAST of PI0028466 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 5.2e-11
Identity = 32/94 (34.04%), Postives = 54/94 (57.45%), Query Frame = 0


Query: 2   VSHDKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQ-------- 61
           +  D  K+  I ++P PT +K+++ FLG   +YR+FI+D++K+A PLT+L +        
Sbjct: 336 IKADPKKVRAISEMPPPTSVKELKRFLGMTSYYRKFIQDYAKVAKPLTNLTRGLYANIKS 395

Query: 62  ---KDVPFVIDDKCKKAFNELKQRFFSTPILQSP 85
                VP  +D+   ++FN+LK    S+ IL  P
Sbjct: 396 SQSSKVPITLDETALQSFNDLKSILCSSEILAFP 429

[Close]

BLAST of PI0028466 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 2.6e-10
Identity = 32/79 (40.51%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 8   KIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPF-VIDDKCKK 67
           KI+ IQK P PT  K++++FLG  G+YR+FI +F+ IA P+T  L+K++     + +   
Sbjct: 426 KIEAIQKYPIPTKPKEIKAFLGLTGYYRKFIPNFADIAKPMTKCLKKNMKIDTTNPEYDS 485

Query: 68  AFNELKQRFFSTPILQSPN 86
           AF +LK      PIL+ P+
Sbjct: 486 AFKKLKYLISEDPILKVPD 504

[Close]

BLAST of PI0028466 vs. ExPASy Swiss-Prot
Match: P03359 (Gag-Pol polyprotein OS=Woolly monkey sarcoma virus OX=11970 GN=pol PE=3 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 4.4e-10
Identity = 29/79 (36.71%), Postives = 48/79 (60.76%), Query Frame = 0


Query: 7    AKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKCKK 66
            A+   + K+P PT  + VR FLG+ GF R +I  F+ +A PL  L ++ +PF+  ++ +K
Sbjct: 922  ARKATVMKIPPPTTPRQVREFLGTAGFCRLWIPGFASLAAPLYPLTKESIPFIWTEEHQK 981

Query: 67   AFNELKQRFFSTPILQSPN 86
            AF+ +K+   S P L  P+
Sbjct: 982  AFDRIKEALLSAPALALPD 1000

[Close]

BLAST of PI0028466 vs. ExPASy Swiss-Prot
Match: P21414 (Gag-Pol polyprotein OS=Gibbon ape leukemia virus OX=11840 GN=pol PE=3 SV=2)

HSP 1 Score: 63.9 bits (154), Expect = 9.9e-10
Identity = 28/79 (35.44%), Postives = 48/79 (60.76%), Query Frame = 0


Query: 7   AKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKCKK 66
           A+   + K+P PT  + VR FLG+ GF R +I  F+ +A PL  L ++ +PF+  ++ ++
Sbjct: 921 ARKATVMKIPVPTTPRQVREFLGTAGFCRLWIPGFASLAAPLYPLTKESIPFIWTEEHQQ 980

Query: 67  AFNELKQRFFSTPILQSPN 86
           AF+ +K+   S P L  P+
Sbjct: 981 AFDHIKKALLSAPALALPD 999

[Close]

BLAST of PI0028466 vs. ExPASy TrEMBL
Match: A0A392MXJ5 (Uncharacterized protein (Fragment) OS=Trifolium medium OX=97028 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-24
Identity = 54/81 (66.67%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 5   DKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKC 64
           D AKIDVI  LP+PTC++++RSFLG  GFYRRFIKDFSKIALPL++LLQKD+ F  DDKC
Sbjct: 94  DPAKIDVISNLPFPTCVREIRSFLGHAGFYRRFIKDFSKIALPLSNLLQKDIIFTFDDKC 153

Query: 65  KKAFNELKQRFFSTPILQSPN 86
           K+AF+ LK+   STP++Q P+
Sbjct: 154 KRAFDCLKKALTSTPVIQPPD 174

[Close]

BLAST of PI0028466 vs. ExPASy TrEMBL
Match: A0A699HVJ5 (Reverse transcriptase domain-containing protein OS=Tanacetum cinerariifolium OX=118510 GN=Tci_447068 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 5.6e-24
Identity = 55/81 (67.90%), Postives = 63/81 (77.78%), Query Frame = 0


Query: 5   DKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKC 64
           DKAKIDVI KLPYPT +K +RSFLG   FY RFIKDFSKIA PLT LL+KD PF  +D+C
Sbjct: 82  DKAKIDVISKLPYPTNVKGIRSFLGHASFYPRFIKDFSKIACPLTKLLEKDTPFEFNDEC 141

Query: 65  KKAFNELKQRFFSTPILQSPN 86
            K FN LK++F  TPI+ SPN
Sbjct: 142 HKVFNSLKEKFTCTPIIMSPN 162

[Close]

BLAST of PI0028466 vs. ExPASy TrEMBL
Match: A0A151QL68 (Transposon Ty3-G Gag-Pol polyprotein OS=Cajanus cajan OX=3821 GN=KK1_049186 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 7.3e-24
Identity = 54/81 (66.67%), Postives = 64/81 (79.01%), Query Frame = 0


Query: 5   DKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKC 64
           DKAKI+VI  LPYPTC+K++RSFLG  GFYRRFIKDFSKIA PL+ LLQKD  F  ++ C
Sbjct: 877 DKAKIEVISSLPYPTCVKEIRSFLGHAGFYRRFIKDFSKIAYPLSQLLQKDAEFDFNEAC 936

Query: 65  KKAFNELKQRFFSTPILQSPN 86
           + AF+ELK R  S PIL +PN
Sbjct: 937 RSAFDELKSRLVSPPILSAPN 957

[Close]

BLAST of PI0028466 vs. ExPASy TrEMBL
Match: A0A2U1NRI2 (Reverse transcriptase OS=Artemisia annua OX=35608 GN=CTI12_AA230240 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 7.3e-24
Identity = 55/81 (67.90%), Postives = 65/81 (80.25%), Query Frame = 0


Query: 5    DKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKC 64
            DKAK+DVI KLP PT +K +RSFLG  GFYRRFIKDFSKIA PLT LL+KD+PF  DD+C
Sbjct: 927  DKAKVDVISKLPPPTNVKGIRSFLGHAGFYRRFIKDFSKIARPLTKLLEKDIPFEFDDEC 986

Query: 65   KKAFNELKQRFFSTPILQSPN 86
            +KAF  LK++   TPI+ SPN
Sbjct: 987  QKAFETLKEKLTCTPIIISPN 1007

[Close]

BLAST of PI0028466 vs. ExPASy TrEMBL
Match: A0A2K3NDP5 (RT_RNaseH_2 domain-containing protein (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g024451 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 7.3e-24
Identity = 55/81 (67.90%), Postives = 64/81 (79.01%), Query Frame = 0


Query: 5   DKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKC 64
           D AKI+V   LPYP+C+ ++RSFLG  GFY RFIKDFSKIALPL++LLQKDV F  DDKC
Sbjct: 147 DPAKINVTSNLPYPSCVSEIRSFLGHAGFYMRFIKDFSKIALPLSNLLQKDVTFTFDDKC 206

Query: 65  KKAFNELKQRFFSTPILQSPN 86
           KKAF+ LK    STPI+Q PN
Sbjct: 207 KKAFDCLKMALTSTPIIQPPN 227

[Close]

BLAST of PI0028466 vs. NCBI nr
Match: XP_021603241.1 (uncharacterized protein LOC110608326 [Manihot esculenta])

HSP 1 Score: 125.9 bits (315), Expect = 1.6e-25
Identity = 54/81 (66.67%), Postives = 69/81 (85.19%), Query Frame = 0


Query: 5   DKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKC 64
           DKAK+DVI+ LPYPTC++D+RSFLG  GFYRRFIKDFS+IA PL  LLQKDVPF  D++C
Sbjct: 450 DKAKVDVIKDLPYPTCIRDIRSFLGHAGFYRRFIKDFSRIAQPLCKLLQKDVPFNFDNEC 509

Query: 65  KKAFNELKQRFFSTPILQSPN 86
           ++AFN LK++  + PI+Q+PN
Sbjct: 510 REAFNTLKEKLVAAPIIQAPN 530

[Close]

BLAST of PI0028466 vs. NCBI nr
Match: XP_028802716.1 (uncharacterized protein LOC114757797 [Prosopis alba])

HSP 1 Score: 124.8 bits (312), Expect = 3.6e-25
Identity = 57/81 (70.37%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 5   DKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKC 64
           DKAKID+I  LPYP+C+KDVRSFLG  GFYRRFI+DFSKIALPL+ LLQKDV FV DD C
Sbjct: 784 DKAKIDIISSLPYPSCVKDVRSFLGHAGFYRRFIQDFSKIALPLSRLLQKDVGFVFDDAC 843

Query: 65  KKAFNELKQRFFSTPILQSPN 86
           + A++ELK+R  + PI+Q PN
Sbjct: 844 RYAWDELKKRLTTPPIIQPPN 864

[Close]

BLAST of PI0028466 vs. NCBI nr
Match: XP_028798815.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC114754213 [Prosopis alba])

HSP 1 Score: 124.4 bits (311), Expect = 4.7e-25
Identity = 57/81 (70.37%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 5    DKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKC 64
            DKAKID+I  LPYP+C+KDVRSFLG  GFYRRFI+DFSKIALPL+ LLQKDV FV DD C
Sbjct: 933  DKAKIDIISSLPYPSCVKDVRSFLGHAGFYRRFIQDFSKIALPLSRLLQKDVGFVFDDAC 992

Query: 65   KKAFNELKQRFFSTPILQSPN 86
            + A++ELK+R  + PI+Q PN
Sbjct: 993  RCAWDELKKRLTTPPIIQPPN 1013

[Close]

BLAST of PI0028466 vs. NCBI nr
Match: XP_028797073.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC114752508 [Prosopis alba])

HSP 1 Score: 124.4 bits (311), Expect = 4.7e-25
Identity = 57/81 (70.37%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 5    DKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKC 64
            DKAKID+I  LPYP+C+KDVRSFLG  GFYRRFI+DFSKIALPL+ LLQKDV FV DD C
Sbjct: 962  DKAKIDIISSLPYPSCVKDVRSFLGHAGFYRRFIQDFSKIALPLSRLLQKDVGFVFDDAC 1021

Query: 65   KKAFNELKQRFFSTPILQSPN 86
            + A++ELK+R  + PI+Q PN
Sbjct: 1022 RCAWDELKKRLTTPPIIQPPN 1042

[Close]

BLAST of PI0028466 vs. NCBI nr
Match: MCH92231.1 (hypothetical protein [Trifolium medium])

HSP 1 Score: 122.1 bits (305), Expect = 2.3e-24
Identity = 54/81 (66.67%), Postives = 68/81 (83.95%), Query Frame = 0


Query: 5   DKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVIDDKC 64
           D AKIDVI  LP+PTC++++RSFLG  GFYRRFIKDFSKIALPL++LLQKD+ F  DDKC
Sbjct: 94  DPAKIDVISNLPFPTCVREIRSFLGHAGFYRRFIKDFSKIALPLSNLLQKDIIFTFDDKC 153

Query: 65  KKAFNELKQRFFSTPILQSPN 86
           K+AF+ LK+   STP++Q P+
Sbjct: 154 KRAFDCLKKALTSTPVIQPPD 174

[Close]

BLAST of PI0028466 vs. TAIR 10
Match: ATMG00860.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 5.9e-10
Identity = 32/84 (38.10%), Postives = 47/84 (55.95%), Query Frame = 0


Query: 2   VSHDKAKIDVIQKLPYPTCLKDVRSFLGSVGFYRRFIKDFSKIALPLTSLLQKDVPFVID 61
           VS D AK++ +   P P    ++R FLG  G+YRRF+K++ KI  PLT LL+K+      
Sbjct: 44  VSADPAKLEAMVGWPEPKNTTELRGFLGLTGYYRRFVKNYGKIVRPLTELLKKN-SLKWT 103

Query: 62  DKCKKAFNELKQRFFSTPILQSPN 86
           +    AF  LK    + P+L  P+
Sbjct: 104 EMAALAFKALKGAVTTLPVLALPD 126

The following BLAST results are available for this feature:

BLAST of PI0028466 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10394	4.0e-11	40.74	Retrovirus-related Pol polyprotein from transposon 412 OS=Drosophila melanogaste...	[more]
Q8I7P9	5.2e-11	34.04	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]
P04323	2.6e-10	40.51	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast...	[more]
P03359	4.4e-10	36.71	Gag-Pol polyprotein OS=Woolly monkey sarcoma virus OX=11970 GN=pol PE=3 SV=2	[more]
P21414	9.9e-10	35.44	Gag-Pol polyprotein OS=Gibbon ape leukemia virus OX=11840 GN=pol PE=3 SV=2	[more]

BLAST of PI0028466 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A392MXJ5	1.1e-24	66.67	Uncharacterized protein (Fragment) OS=Trifolium medium OX=97028 PE=4 SV=1	[more]
A0A699HVJ5	5.6e-24	67.90	Reverse transcriptase domain-containing protein OS=Tanacetum cinerariifolium OX=...	[more]
A0A151QL68	7.3e-24	66.67	Transposon Ty3-G Gag-Pol polyprotein OS=Cajanus cajan OX=3821 GN=KK1_049186 PE=4...	[more]
A0A2U1NRI2	7.3e-24	67.90	Reverse transcriptase OS=Artemisia annua OX=35608 GN=CTI12_AA230240 PE=4 SV=1	[more]
A0A2K3NDP5	7.3e-24	67.90	RT_RNaseH_2 domain-containing protein (Fragment) OS=Trifolium pratense OX=57577 ...	[more]

BLAST of PI0028466 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_021603241.1	1.6e-25	66.67	uncharacterized protein LOC110608326 [Manihot esculenta]	[more]
XP_028802716.1	3.6e-25	70.37	uncharacterized protein LOC114757797 [Prosopis alba]	[more]
XP_028798815.1	4.7e-25	70.37	LOW QUALITY PROTEIN: uncharacterized protein LOC114754213 [Prosopis alba]	[more]
XP_028797073.1	4.7e-25	70.37	LOW QUALITY PROTEIN: uncharacterized protein LOC114752508 [Prosopis alba]	[more]
MCH92231.1	2.3e-24	66.67	hypothetical protein [Trifolium medium]	[more]

BLAST of PI0028466 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00860.1	5.9e-10	38.10	DNA/RNA polymerases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043128	Reverse transcriptase/Diguanylate cyclase domain	GENE3D	3.30.70.270		coord: 6..85 e-value: 7.9E-28 score: 98.3
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 4..73
None	No IPR available	PANTHER	PTHR24559:SF345	SUBFAMILY NOT NAMED	coord: 4..73
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 2..85

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0028466.1	PI0028466.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003964	RNA-directed DNA polymerase activity