MS018159 (gene) Bitter gourd (TR) v1

BLAST of MS018159 vs. NCBI nr
Match: XP_022159299.1 (protein NRT1/ PTR FAMILY 4.4-like [Momordica charantia])

HSP 1 Score: 974.9 bits (2519), Expect = 2.8e-280
Identity = 508/547 (92.87%), Postives = 512/547 (93.60%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT
Sbjct: 20  VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 79

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 120
           NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT
Sbjct: 80  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 139

Query: 121 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAY 180
           STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQ KKLSTFFNCAY
Sbjct: 140 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQLKKLSTFFNCAY 199

Query: 181 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 240
           FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP
Sbjct: 200 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 259

Query: 241 IAQVF---FNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDEG 300
           IAQVF   F        S S  L         +N  L      +  FLDKACIKSEDDEG
Sbjct: 260 IAQVFVAAFTKRKQICPSNSEML------HGTENGLLKLLHTDKFRFLDKACIKSEDDEG 319

Query: 301 REESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQ 360
           REESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQ
Sbjct: 320 REESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQ 379

Query: 361 IPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAA 420
           IPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAA
Sbjct: 380 IPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAA 439

Query: 421 LVETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMT 480
           LVETKRRNSS AG+PPLSILWI+PQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMT
Sbjct: 440 LVETKRRNSSAAGAPPLSILWISPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMT 499

Query: 481 YCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYL 540
           YCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYL
Sbjct: 500 YCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYL 559

Query: 541 FWANRYS 545
           FWANRYS
Sbjct: 560 FWANRYS 560

BLAST of MS018159 vs. NCBI nr
Match: KAG7037184.1 (Protein NRT1/ PTR FAMILY 4.4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 831.2 bits (2146), Expect = 5.0e-237
Identity = 443/545 (81.28%), Postives = 470/545 (86.24%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGR C+SN HGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT
Sbjct: 24  VDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 83

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 120
           NFVGTVF LSLLGGF+SDSYLGSFRTML+FG +ELSGF+LLTVQAHYP LRP  CDM  T
Sbjct: 84  NFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPACDM-GT 143

Query: 121 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAY 180
              C+EA G EA M F ALYLVALGSGCLKPNIISHGADQF ++ P QF+KLSTFFNCAY
Sbjct: 144 GQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFFNCAY 203

Query: 181 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 240
           FAFCTGEL ALTLLVW+QTH GMDVGF VSTAAMLLGLI LL+ TS +RN+PPRGTIFTP
Sbjct: 204 FAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGTIFTP 263

Query: 241 IAQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDE-GRE 300
           IAQVF   A +    +    S +  R   QN  +         FLDKACIKSE+   G E
Sbjct: 264 IAQVFV-AAFVKRKQICPSNSEMLHR--SQNGVVKLLHTDNFRFLDKACIKSEEGRVGSE 323

Query: 301 ESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQIP 360
           ESPWKLCTV QVEQVKIILS+IPIFACTIIFNTILAQLQTFSVQQG +M++ LT  FQIP
Sbjct: 324 ESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNSFQIP 383

Query: 361 PASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAALV 420
           PASLQ+IPY+ILIFLVPLYETAFVPLARR+T   SGISPLQRVGTGLFVATFSMVSAALV
Sbjct: 384 PASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTGLFVATFSMVSAALV 443

Query: 421 ETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC 480
           E KRRNSS  GS  LSILWIAPQF+IFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC
Sbjct: 444 EKKRRNSSIVGS-SLSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC 503

Query: 481 SYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYLFW 540
           SYSFGFYLSSLLVSLVNKIT GS+ GGWLSHNDLNNDRLDLFYWLLAGLS VNFFNYLFW
Sbjct: 504 SYSFGFYLSSLLVSLVNKITGGSSGGGWLSHNDLNNDRLDLFYWLLAGLSLVNFFNYLFW 563

Query: 541 ANRYS 545
           ANRYS
Sbjct: 564 ANRYS 563

BLAST of MS018159 vs. NCBI nr
Match: XP_038889646.1 (protein NRT1/ PTR FAMILY 4.4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 830.5 bits (2144), Expect = 8.5e-237
Identity = 439/547 (80.26%), Postives = 469/547 (85.74%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGR C+SN HGGM AAAFVLGLQAFEMMAIAAVGNNLITYVF++MHFPLSKSANVVT
Sbjct: 9   VDWRGRGCNSNKHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVT 68

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMA-A 120
           NFVGTVF LSLLGGF+SDSYLGSFRTML+FG +EL+GFTLLTVQAHYPGLRPA C+M   
Sbjct: 69  NFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTLLTVQAHYPGLRPAACEMGRK 128

Query: 121 TSTPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCA 180
               C+EA G EA MFF ALYLVALGSGCLKPNIISHGADQF ++DP QFKKLSTFFNCA
Sbjct: 129 EGHECIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFQRQDPTQFKKLSTFFNCA 188

Query: 181 YFAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFT 240
           YFAFCTGEL ALTLLVWLQTH+GMD+GFGVSTA MLL LI LLS TS YRN PPRG+IFT
Sbjct: 189 YFAFCTGELIALTLLVWLQTHSGMDLGFGVSTAVMLLALISLLSGTSFYRNNPPRGSIFT 248

Query: 241 PIAQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDE--G 300
           PIAQVF   A+ S   L +  S        QN  +      +  FLDKACI+SE+     
Sbjct: 249 PIAQVFV--AAYSKRKLQICPSNSEMLHGSQNGVVKLLHTHKFRFLDKACIRSEEGRLGI 308

Query: 301 REESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQ 360
           REESPWKLCT+ QVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG SM++WLT  FQ
Sbjct: 309 REESPWKLCTIKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTWLTNSFQ 368

Query: 361 IPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAA 420
           IPPASLQAIPYLILIFLVPLYET FVPLA RLTG  SGISPLQRVGTGLFVATFSMVSAA
Sbjct: 369 IPPASLQAIPYLILIFLVPLYETVFVPLAHRLTGIDSGISPLQRVGTGLFVATFSMVSAA 428

Query: 421 LVETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMT 480
           LVE KRR S+++ S  LSILWIAPQFVIFG+SELFTAVGL+EFFYKQSVEGMQSFLTAMT
Sbjct: 429 LVEQKRRTSTSSSS--LSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGMQSFLTAMT 488

Query: 481 YCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYL 540
           YCSYSFGFYLSSLLVSLVNKI     +GGWLSHNDLNNDRLDLFYWLLAGLS VNFFNYL
Sbjct: 489 YCSYSFGFYLSSLLVSLVNKI-----SGGWLSHNDLNNDRLDLFYWLLAGLSLVNFFNYL 546

Query: 541 FWANRYS 545
           FWANR+S
Sbjct: 549 FWANRFS 546

BLAST of MS018159 vs. NCBI nr
Match: XP_022932490.1 (protein NRT1/ PTR FAMILY 4.4-like [Cucurbita moschata])

HSP 1 Score: 830.1 bits (2143), Expect = 1.1e-236
Identity = 442/545 (81.10%), Postives = 470/545 (86.24%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGR C+SN HGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT
Sbjct: 24  VDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 83

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 120
           NFVGTVF LSLLGGF+SDSYLGSFRTML+FG +ELSGF+LLTVQAHYP LRP  CDM  T
Sbjct: 84  NFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPACDM-GT 143

Query: 121 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAY 180
              C+EA G EA M F ALYLVALGSGCLKPNIISHGADQF ++ P QF+KLSTFFNCAY
Sbjct: 144 GQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFFNCAY 203

Query: 181 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 240
           FAFCTGEL ALTLLVW+QTH GMDVGF VSTAAMLLGLI LL+ TS +RN+PPRGTIFTP
Sbjct: 204 FAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGTIFTP 263

Query: 241 IAQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDE-GRE 300
           IAQVF   A +    +    S +  R   QN  +         FLDKACIKSE+   G E
Sbjct: 264 IAQVFV-AAFVKRKQICPSNSEMLHR--SQNGVVKLLHTDNFRFLDKACIKSEEGRVGSE 323

Query: 301 ESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQIP 360
           ESPWKLCTV QVEQVKIILS+IPIFACTIIFNTILAQLQTFSVQQG +M++ LT  FQIP
Sbjct: 324 ESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNSFQIP 383

Query: 361 PASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAALV 420
           PASLQ+IPY+ILIFLVPLYETAFVPLARR+T   SGISPLQRVGTGLFVATFSMVSAALV
Sbjct: 384 PASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTGLFVATFSMVSAALV 443

Query: 421 ETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC 480
           E KRRNSS  GS  LSILWIAPQF+IFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC
Sbjct: 444 EKKRRNSSIVGS-SLSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC 503

Query: 481 SYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYLFW 540
           SYSFGFYLSSLLVSLVNKIT G++ GGWLSHNDLNNDRLDLFYWLLAGLS VNFFNYLFW
Sbjct: 504 SYSFGFYLSSLLVSLVNKITGGASGGGWLSHNDLNNDRLDLFYWLLAGLSLVNFFNYLFW 563

Query: 541 ANRYS 545
           ANRYS
Sbjct: 564 ANRYS 563

BLAST of MS018159 vs. NCBI nr
Match: XP_004141613.1 (protein NRT1/ PTR FAMILY 4.4 [Cucumis sativus] >KGN52605.1 hypothetical protein Csa_008579 [Cucumis sativus])

HSP 1 Score: 829.3 bits (2141), Expect = 1.9e-236
Identity = 440/552 (79.71%), Postives = 473/552 (85.69%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGRAC+ N HGGM AAAFVLGLQAFEMMAIAAVGNNLITYVF++MHFPLSKSANVVT
Sbjct: 10  VDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVT 69

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMA-- 120
           NFVGTVF LSLLGGF+SDSYLGSFRTML+FG +EL+GFT+LT+QAHYPGLRP+PC+M   
Sbjct: 70  NFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEMGRK 129

Query: 121 --ATSTPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFF 180
                  C+EA G EA MFF ALYLVALGSGCLKPNIISHGADQF KEDPKQFKKLSTFF
Sbjct: 130 LDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLSTFF 189

Query: 181 NCAYFAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGT 240
           NCAYFAFCTGEL ALTLLVW+QTH+GMD+GFGVSTAAMLL LI LLS TS YRN PPRG+
Sbjct: 190 NCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPPRGS 249

Query: 241 IFTPIAQVFFNHASLSLHSLSLFLSYLWDRFS-DQNSFLMKYSCKRRLFLDKACIKSEDD 300
           IFTPIAQV    A+    +L +  S      +  QN  +      +  FLDKACIKSE++
Sbjct: 250 IFTPIAQVLV--AAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKACIKSEEE 309

Query: 301 ---EGREESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWL 360
                REESPWKLCTV QVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG SM++WL
Sbjct: 310 RLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTWL 369

Query: 361 TPKFQIPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFS 420
           T  FQIPPASLQAIPY+ILIFLVPLYET FVPL RRLT   SGISPLQRVGTGLFVATFS
Sbjct: 370 TNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTGLFVATFS 429

Query: 421 MVSAALVETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSF 480
           MVSAALVE KRRNSS++    LSILWIAPQFVIFG+SELFTAVGL+EFFYKQSVEGMQSF
Sbjct: 430 MVSAALVEQKRRNSSSS----LSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGMQSF 489

Query: 481 LTAMTYCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVN 540
           LTAMTYCSYSFGFYLSSLLVSLVNKI     +GGWLSHNDLNNDRLDLFYWLLAGLSFVN
Sbjct: 490 LTAMTYCSYSFGFYLSSLLVSLVNKI-----SGGWLSHNDLNNDRLDLFYWLLAGLSFVN 549

Query: 541 FFNYLFWANRYS 545
           FFNYLFWANR+S
Sbjct: 550 FFNYLFWANRFS 550

BLAST of MS018159 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 2.2e-203
Identity = 376/547 (68.74%), Postives = 435/547 (79.52%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VD+RGR C  + HGG  AA FVLG QAFEMMAIAAVGNNLITYVF +MHFPLSKSAN+VT
Sbjct: 27  VDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVT 86

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 120
           NF+GTVFLLSLLGGF+SDSYLGSFRTMLVFG++E+SGF LL+VQAH P LRP  C+M +T
Sbjct: 87  NFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKST 146

Query: 121 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAY 180
           +  CVEA+G +AA  + AL LVALGSGCLKPNIISHGA+QF ++D    +KLS+FFN AY
Sbjct: 147 TIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAY 206

Query: 181 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 240
           FAF  G+L ALTLLVW+QTH+GMDVGFGVS A M  G+I L++ TS YRN PP G+IFTP
Sbjct: 207 FAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTP 266

Query: 241 IAQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDEGREE 300
           IAQVF    +          + +    +D           +  FLDKACIK++      E
Sbjct: 267 IAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIKTQGK--AME 326

Query: 301 SPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQIPP 360
           SPW+LCT+ QV QVKI+LSVIPIFACTIIFNTILAQLQTFSVQQG+SM++ +T  FQIPP
Sbjct: 327 SPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPP 386

Query: 361 ASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAALVE 420
           ASLQAIPY+ILIF VPLYET FVPLAR+LTG  SGISPLQR+GTGLF+ATFSMV+AALVE
Sbjct: 387 ASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVE 446

Query: 421 TKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYCS 480
            KRR S    +  LSI WIAPQF+IFG+SE+FTAVGLVEFFYKQS + MQSFLTAMTYCS
Sbjct: 447 KKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCS 506

Query: 481 YSFGFYLSSLLVSLVNKITRGSAAG---GWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYL 540
           YSFGFYLSS+LVS VN++T  + +G   GWL  NDLN DRLD FYWLLA LSF+NFFNYL
Sbjct: 507 YSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYL 566

Query: 541 FWANRYS 545
           FW+  YS
Sbjct: 567 FWSRWYS 568

BLAST of MS018159 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 1.3e-187
Identity = 348/544 (63.97%), Postives = 421/544 (77.39%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGR  + N HGGM AA FVLGLQAFE+M IAAVGNNLITYV  +MHFPLSK+AN+VT
Sbjct: 30  VDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVT 89

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 120
           NFVGT+F+ +LLGG++SD++LGSF T+++FG VELSGF LL+VQAH P L+P  C+    
Sbjct: 90  NFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLID 149

Query: 121 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAY 180
            T C EA G +A +FF+ALYLVALGSGC+KPN+I+HGADQF +  PKQ K+LS++FN AY
Sbjct: 150 QT-CEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAY 209

Query: 181 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 240
           FAF  GEL ALTLLVW+QTH+GMD+GFGVS AAM +G+I L+S T  +RN  PR +IFTP
Sbjct: 210 FAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTP 269

Query: 241 IAQVF---FNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDEG 300
           IA V         L+  S    L       +D           R  FLDKACIK + D  
Sbjct: 270 IAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQ-DTN 329

Query: 301 REESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQ 360
            +ESPW+LCTV QVEQVK ++S++PIFA TI+FNTILAQLQTFSVQQG+SM++ L+  F 
Sbjct: 330 TKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFH 389

Query: 361 IPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAA 420
           IPPASLQAIPY++LIFLVPLY++  VP AR+LTG  SGI PL R+G GLF++TFSMVSAA
Sbjct: 390 IPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAA 449

Query: 421 LVETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMT 480
           ++E KRR+SS      LSI WI PQF+IFGISE+FTAVGL+EFFYKQS +GM+SFL A+T
Sbjct: 450 MLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALT 509

Query: 481 YCSYSFGFYLSSLLVSLVNKITRGSA-AGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNY 540
           YCSYSFGFY SS+LVS+VNKIT  S  + GWL  NDLN DRLDLFYWLLA LS +NF +Y
Sbjct: 510 YCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSY 569

BLAST of MS018159 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 4.5e-108
Identity = 237/571 (41.51%), Postives = 332/571 (58.14%), Query Frame = 0

Query: 2   DWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVTN 61
           DWR RA     HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH   SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 62  FVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAATS 121
           F+GT FLL+LLGGF+SD++  +F+  L+   +E  G  +LT+QA  P L P  CD    S
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCD----S 134

Query: 122 TPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAYF 181
             C E SG +AAM FV LYLVALG G +K ++ SHGA+QF +  PK  K+ STFFN  YF
Sbjct: 135 PTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFN--YF 194

Query: 182 AFC--TGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFT 241
            FC   G L A+T +VWL+ + G + GFGVST A+ + ++  LS +  YRN  P G+  T
Sbjct: 195 VFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLT 254

Query: 242 PIAQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDE--- 301
            I +V    +     S S   +      S  N  + K   K+ +       K   +E   
Sbjct: 255 TILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSK--GKKEVESQGELEKPRQEEALP 314

Query: 302 --------------GREESPWKL---CTVAQVEQVKIILSVIPIFACTIIFNTILAQLQT 361
                           +E P      CTV QVE VKI+L ++PIFACTI+ N  LAQL T
Sbjct: 315 PRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLST 374

Query: 362 FSVQQGTSMDSWLTPKFQIPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPL 421
           FSVQQ  SM++ +    +IPPASL   P + ++ L P+Y+   +P AR+ T   +G++ L
Sbjct: 375 FSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHL 434

Query: 422 QRVGTGLFVATFSMVSAALVETKRRNSS-------TAGSPPLSILWIAPQFVIFGISELF 481
           QR+G GL ++  +M  AALVE KR+  +       +  + P++ LWIA Q++  G ++LF
Sbjct: 435 QRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLF 494

Query: 482 TAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHND 541
           T  GL+E+F+ ++   M+S  T++++ S + G+YLSS++VS+VN IT  S    WL    
Sbjct: 495 TLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKS 554

Query: 542 LNNDRLDLFYWLLAGLSFVNFFNYLFWANRY 544
           +N  +LD FYWL+  LS  NF +YLFWA RY
Sbjct: 555 INRYKLDYFYWLMCVLSAANFLHYLFWAMRY 576

BLAST of MS018159 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 2.5e-106
Identity = 219/549 (39.89%), Postives = 330/549 (60.11%), Query Frame = 0

Query: 2   DWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVTN 61
           DWR +A     HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH  L++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 62  FVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAATS 121
           F+ T FLL+LLGGF++D++  +F   L+   +E  G  LLT+QA  P L P PC  +A +
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSA-A 136

Query: 122 TPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAYF 181
             C    G +AA  FV LYLV+LG G +K ++ SHGA+QF +  PK  K+ STFFN   F
Sbjct: 137 LRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVF 196

Query: 182 AFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTPI 241
               G L A+T +VW++ + G + GFGVST ++ L ++  L  +  Y+N  PRG+  T I
Sbjct: 197 CLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTI 256

Query: 242 AQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDEGREES 301
            +V    + +S  S +    +       ++    + +  + L     C+ ++  EG+   
Sbjct: 257 FKVLLAASIVSCSSKTSSNHFTSREVQSEHE---EKTPSQSLTNSLTCL-NKAIEGKTHH 316

Query: 302 PWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQIPPA 361
            W  CTV QVE VKI+L ++PIF CTI+ N  LAQL T+SV Q  +M+  +   F +P A
Sbjct: 317 IWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV-NFNVPSA 376

Query: 362 SLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAALVET 421
           SL   P + ++ L P Y+   +P AR++T +  GI+ LQR+G GL ++  +M  AALVE 
Sbjct: 377 SLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVEL 436

Query: 422 KRRN-SSTAG------SPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLT 481
           KR+  +  AG      + P++ LWIA Q++  G ++LFT  GL+EFF+ ++   M+S  T
Sbjct: 437 KRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLAT 496

Query: 482 AMTYCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFF 541
           ++++ S + G+YLSS++V +VN++T+ +    WL    LN +RLDLFYWL+  LS VNF 
Sbjct: 497 SLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGEK-LNRNRLDLFYWLMCVLSVVNFL 556

Query: 542 NYLFWANRY 544
           +YLFWA RY
Sbjct: 557 HYLFWAKRY 558

BLAST of MS018159 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 8.9e-96
Identity = 205/555 (36.94%), Postives = 317/555 (57.12%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           +D   +  + N  G   A  F+LG +  E +A   +  NLI Y+  QM+     ++  V+
Sbjct: 14  LDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVS 73

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 120
           N+ GT +   L+G F++D+YLG + T+  F ++ ++G TLLT+ A  PGL P        
Sbjct: 74  NWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTP------TC 133

Query: 121 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAY 180
           S     A+  + A+ F+ALYL+ALG+G +KP + S GADQF   D K+ +  S+FFN  Y
Sbjct: 134 SGETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFY 193

Query: 181 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 240
           F    G + A ++LVW+Q + G   G GV T AM + ++   + ++ YR   P G+  T 
Sbjct: 194 FVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTR 253

Query: 241 IAQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYS---CKRRLFLDKACIKSEDDE- 300
           + QV       S   +    S L++    ++S +        K   F DKA +++E D  
Sbjct: 254 MLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESDNK 313

Query: 301 -GREESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPK 360
              + S WKLCTV QVE++K ++ ++PI+A  I+F ++ +Q+ T  V QG ++D  + P 
Sbjct: 314 GAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPN 373

Query: 361 FQIPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVS 420
           F+IP ASL     L ++F  P+Y+   VP AR+ TG   G + LQR+G GL ++ FSMVS
Sbjct: 374 FKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVS 433

Query: 421 AALVETKR------RNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGM 480
           A ++E  R       N     + P++I W  PQ+ + G +E+FT +G +EFFY Q+ + M
Sbjct: 434 AGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAM 493

Query: 481 QSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLS 540
           +S  +A++  + +FG YLS+ LV+LV K+TR     GW++ N LNN  LD F+WLLAGLS
Sbjct: 494 RSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKN-LNNGHLDYFFWLLAGLS 553

Query: 541 FVNFFNYLFWANRYS 545
           F+NF  YL+ A  Y+
Sbjct: 554 FLNFLVYLWIAKWYT 561

BLAST of MS018159 vs. ExPASy TrEMBL
Match: A0A6J1E215 (protein NRT1/ PTR FAMILY 4.4-like OS=Momordica charantia OX=3673 GN=LOC111025710 PE=3 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.3e-280
Identity = 508/547 (92.87%), Postives = 512/547 (93.60%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT
Sbjct: 20  VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 79

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 120
           NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT
Sbjct: 80  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 139

Query: 121 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAY 180
           STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQ KKLSTFFNCAY
Sbjct: 140 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQLKKLSTFFNCAY 199

Query: 181 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 240
           FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP
Sbjct: 200 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 259

Query: 241 IAQVF---FNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDEG 300
           IAQVF   F        S S  L         +N  L      +  FLDKACIKSEDDEG
Sbjct: 260 IAQVFVAAFTKRKQICPSNSEML------HGTENGLLKLLHTDKFRFLDKACIKSEDDEG 319

Query: 301 REESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQ 360
           REESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQ
Sbjct: 320 REESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQ 379

Query: 361 IPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAA 420
           IPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAA
Sbjct: 380 IPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAA 439

Query: 421 LVETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMT 480
           LVETKRRNSS AG+PPLSILWI+PQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMT
Sbjct: 440 LVETKRRNSSAAGAPPLSILWISPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMT 499

Query: 481 YCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYL 540
           YCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYL
Sbjct: 500 YCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYL 559

Query: 541 FWANRYS 545
           FWANRYS
Sbjct: 560 FWANRYS 560

BLAST of MS018159 vs. ExPASy TrEMBL
Match: A0A6J1F2C4 (protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita moschata OX=3662 GN=LOC111438893 PE=3 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 5.4e-237
Identity = 442/545 (81.10%), Postives = 470/545 (86.24%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGR C+SN HGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT
Sbjct: 24  VDWRGRPCTSNKHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 83

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 120
           NFVGTVF LSLLGGF+SDSYLGSFRTML+FG +ELSGF+LLTVQAHYP LRP  CDM  T
Sbjct: 84  NFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPPACDM-GT 143

Query: 121 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAY 180
              C+EA G EA M F ALYLVALGSGCLKPNIISHGADQF ++ P QF+KLSTFFNCAY
Sbjct: 144 GQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFFNCAY 203

Query: 181 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 240
           FAFCTGEL ALTLLVW+QTH GMDVGF VSTAAMLLGLI LL+ TS +RN+PPRGTIFTP
Sbjct: 204 FAFCTGELIALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGTIFTP 263

Query: 241 IAQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDE-GRE 300
           IAQVF   A +    +    S +  R   QN  +         FLDKACIKSE+   G E
Sbjct: 264 IAQVFV-AAFVKRKQICPSNSEMLHR--SQNGVVKLLHTDNFRFLDKACIKSEEGRVGSE 323

Query: 301 ESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQIP 360
           ESPWKLCTV QVEQVKIILS+IPIFACTIIFNTILAQLQTFSVQQG +M++ LT  FQIP
Sbjct: 324 ESPWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTSLTNSFQIP 383

Query: 361 PASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAALV 420
           PASLQ+IPY+ILIFLVPLYETAFVPLARR+T   SGISPLQRVGTGLFVATFSMVSAALV
Sbjct: 384 PASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTGLFVATFSMVSAALV 443

Query: 421 ETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC 480
           E KRRNSS  GS  LSILWIAPQF+IFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC
Sbjct: 444 EKKRRNSSIVGS-SLSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC 503

Query: 481 SYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYLFW 540
           SYSFGFYLSSLLVSLVNKIT G++ GGWLSHNDLNNDRLDLFYWLLAGLS VNFFNYLFW
Sbjct: 504 SYSFGFYLSSLLVSLVNKITGGASGGGWLSHNDLNNDRLDLFYWLLAGLSLVNFFNYLFW 563

Query: 541 ANRYS 545
           ANRYS
Sbjct: 564 ANRYS 563

BLAST of MS018159 vs. ExPASy TrEMBL
Match: A0A0A0KSN5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G647280 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 9.2e-237
Identity = 440/552 (79.71%), Postives = 473/552 (85.69%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGRAC+ N HGGM AAAFVLGLQAFEMMAIAAVGNNLITYVF++MHFPLSKSANVVT
Sbjct: 10  VDWRGRACNPNRHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVT 69

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMA-- 120
           NFVGTVF LSLLGGF+SDSYLGSFRTML+FG +EL+GFT+LT+QAHYPGLRP+PC+M   
Sbjct: 70  NFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEMGRK 129

Query: 121 --ATSTPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFF 180
                  C+EA G EA MFF ALYLVALGSGCLKPNIISHGADQF KEDPKQFKKLSTFF
Sbjct: 130 LDEEDDQCIEAKGYEALMFFSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLSTFF 189

Query: 181 NCAYFAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGT 240
           NCAYFAFCTGEL ALTLLVW+QTH+GMD+GFGVSTAAMLL LI LLS TS YRN PPRG+
Sbjct: 190 NCAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPPRGS 249

Query: 241 IFTPIAQVFFNHASLSLHSLSLFLSYLWDRFS-DQNSFLMKYSCKRRLFLDKACIKSEDD 300
           IFTPIAQV    A+    +L +  S      +  QN  +      +  FLDKACIKSE++
Sbjct: 250 IFTPIAQVLV--AAYRKRNLQMCPSNSEMVLNGTQNGIVKLIHTDKFRFLDKACIKSEEE 309

Query: 301 ---EGREESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWL 360
                REESPWKLCTV QVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG SM++WL
Sbjct: 310 RLGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTWL 369

Query: 361 TPKFQIPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFS 420
           T  FQIPPASLQAIPY+ILIFLVPLYET FVPL RRLT   SGISPLQRVGTGLFVATFS
Sbjct: 370 TNSFQIPPASLQAIPYIILIFLVPLYETVFVPLTRRLTSIDSGISPLQRVGTGLFVATFS 429

Query: 421 MVSAALVETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSF 480
           MVSAALVE KRRNSS++    LSILWIAPQFVIFG+SELFTAVGL+EFFYKQSVEGMQSF
Sbjct: 430 MVSAALVEQKRRNSSSS----LSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGMQSF 489

Query: 481 LTAMTYCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVN 540
           LTAMTYCSYSFGFYLSSLLVSLVNKI     +GGWLSHNDLNNDRLDLFYWLLAGLSFVN
Sbjct: 490 LTAMTYCSYSFGFYLSSLLVSLVNKI-----SGGWLSHNDLNNDRLDLFYWLLAGLSFVN 549

Query: 541 FFNYLFWANRYS 545
           FFNYLFWANR+S
Sbjct: 550 FFNYLFWANRFS 550

BLAST of MS018159 vs. ExPASy TrEMBL
Match: A0A6J1ID44 (protein NRT1/ PTR FAMILY 4.4-like OS=Cucurbita maxima OX=3661 GN=LOC111472001 PE=3 SV=1)

HSP 1 Score: 827.8 bits (2137), Expect = 2.7e-236
Identity = 438/545 (80.37%), Postives = 468/545 (85.87%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGR C+SN HGGM AAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT
Sbjct: 24  VDWRGRPCTSNKHGGMTAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 83

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 120
           NFVGTVF LSLLGGF+SDSYLGSFRTML+FG +ELSGF+LLTVQAHYP LRP  CDM  T
Sbjct: 84  NFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFLELSGFSLLTVQAHYPRLRPLACDM-GT 143

Query: 121 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAY 180
              C+EA G EA M F ALYLVALGSGCLKPNIISHGADQF ++ P QF+KLSTFFNCAY
Sbjct: 144 GQQCMEAKGYEALMLFTALYLVALGSGCLKPNIISHGADQFRRQHPTQFQKLSTFFNCAY 203

Query: 181 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 240
           FAFCTGEL ALTLLVW+QTH GMDVGF VSTAAMLLGLI LL+ TS +RN+PPRGTIFTP
Sbjct: 204 FAFCTGELLALTLLVWVQTHYGMDVGFTVSTAAMLLGLISLLAGTSFFRNSPPRGTIFTP 263

Query: 241 IAQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDE-GRE 300
           IAQVF    +  +    ++ S        QN  +      +  FLDKACIKSE+   G E
Sbjct: 264 IAQVF---VAAFVKRKQIYPSNSEMLHRSQNGVVKLLHTDKFRFLDKACIKSEEGRVGSE 323

Query: 301 ESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQIP 360
           ES WKLCTV QVEQVKIILS+IPIFACTIIFNTILAQLQTFSVQQG +M++ LT  FQIP
Sbjct: 324 ESQWKLCTVTQVEQVKIILSLIPIFACTIIFNTILAQLQTFSVQQGAAMNTCLTNSFQIP 383

Query: 361 PASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAALV 420
           PASLQ+IPY+ILIFLVPLYETAFVPLARR+T   SGISPLQRVGTGLFVATFSMVSAALV
Sbjct: 384 PASLQSIPYIILIFLVPLYETAFVPLARRITHTNSGISPLQRVGTGLFVATFSMVSAALV 443

Query: 421 ETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC 480
           E KRRNSS  GS  LSILWIAPQF+IFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC
Sbjct: 444 EKKRRNSSVVGS-SLSILWIAPQFIIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYC 503

Query: 481 SYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYLFW 540
           SYSFGFYLSSLLVSLVNKIT G++ GGWLSHNDLNNDRLDLFYWLLAGLS VNFFNYLFW
Sbjct: 504 SYSFGFYLSSLLVSLVNKITGGASGGGWLSHNDLNNDRLDLFYWLLAGLSLVNFFNYLFW 563

Query: 541 ANRYS 545
           ANRYS
Sbjct: 564 ANRYS 563

BLAST of MS018159 vs. ExPASy TrEMBL
Match: A0A5D3BQJ5 (Protein NRT1/ PTR FAMILY 4.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001460 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 4.5e-236
Identity = 436/551 (79.13%), Postives = 473/551 (85.84%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGRAC+ N HGGM AAAFVLGLQAFEMMAIAAVGNNLITYVF++MHFPLSKSANVVT
Sbjct: 10  VDWRGRACNPNKHGGMGAAAFVLGLQAFEMMAIAAVGNNLITYVFSEMHFPLSKSANVVT 69

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAA- 120
           NFVGTVF LSLLGGF+SDSYLGSFRTML+FG +EL+GFT+LT+QAHYPGLRP+PC+M   
Sbjct: 70  NFVGTVFFLSLLGGFLSDSYLGSFRTMLIFGFIELAGFTVLTIQAHYPGLRPSPCEMGRK 129

Query: 121 --TSTPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFN 180
                 C+EA G EA MF+ ALYLVALGSGCLKPNIISHGADQF KEDPKQFKKLSTFFN
Sbjct: 130 LDEEDECIEAKGYEALMFYSALYLVALGSGCLKPNIISHGADQFRKEDPKQFKKLSTFFN 189

Query: 181 CAYFAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTI 240
           CAYFAFCTGEL ALTLLVW+QTH+GMD+GFGVSTAAMLL LI LLS TS YRN PPRG+I
Sbjct: 190 CAYFAFCTGELIALTLLVWVQTHSGMDLGFGVSTAAMLLALISLLSGTSFYRNNPPRGSI 249

Query: 241 FTPIAQVFF-NHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDD- 300
           FTPIAQV    +   +L + +     + +    QN  +      +  FLDKACI+SE++ 
Sbjct: 250 FTPIAQVLVAAYRKRNLQTCASNSEMVLN--GTQNGIVKLIHTDKFRFLDKACIRSEEER 309

Query: 301 --EGREESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLT 360
               REESPWKLCTV QVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQG SM++WLT
Sbjct: 310 LGSIREESPWKLCTVKQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGASMNTWLT 369

Query: 361 PKFQIPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSM 420
             FQIPPASLQAIPY+ILIFLVPLYET FVPLARRLT   SGISPLQRVGTGLFVATFSM
Sbjct: 370 NSFQIPPASLQAIPYIILIFLVPLYETVFVPLARRLTSIDSGISPLQRVGTGLFVATFSM 429

Query: 421 VSAALVETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFL 480
           VSAALVE KRRNSS++    LSILWIAPQFVIFG+SELFTAVGL+EFFYKQSVEGMQSFL
Sbjct: 430 VSAALVEQKRRNSSSS----LSILWIAPQFVIFGVSELFTAVGLIEFFYKQSVEGMQSFL 489

Query: 481 TAMTYCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNF 540
           TAMTYCSYSFGFYLSSLLVSLVNKI     +GGWLSHNDLN DRLDLFYWLLAGLSFVNF
Sbjct: 490 TAMTYCSYSFGFYLSSLLVSLVNKI-----SGGWLSHNDLNKDRLDLFYWLLAGLSFVNF 549

Query: 541 FNYLFWANRYS 545
           FNYLFWANR+S
Sbjct: 550 FNYLFWANRFS 549

BLAST of MS018159 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 709.9 bits (1831), Expect = 1.6e-204
Identity = 376/547 (68.74%), Postives = 435/547 (79.52%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VD+RGR C  + HGG  AA FVLG QAFEMMAIAAVGNNLITYVF +MHFPLSKSAN+VT
Sbjct: 27  VDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVT 86

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 120
           NF+GTVFLLSLLGGF+SDSYLGSFRTMLVFG++E+SGF LL+VQAH P LRP  C+M +T
Sbjct: 87  NFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKST 146

Query: 121 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAY 180
           +  CVEA+G +AA  + AL LVALGSGCLKPNIISHGA+QF ++D    +KLS+FFN AY
Sbjct: 147 TIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAY 206

Query: 181 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 240
           FAF  G+L ALTLLVW+QTH+GMDVGFGVS A M  G+I L++ TS YRN PP G+IFTP
Sbjct: 207 FAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTP 266

Query: 241 IAQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDEGREE 300
           IAQVF    +          + +    +D           +  FLDKACIK++      E
Sbjct: 267 IAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIKTQGK--AME 326

Query: 301 SPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQIPP 360
           SPW+LCT+ QV QVKI+LSVIPIFACTIIFNTILAQLQTFSVQQG+SM++ +T  FQIPP
Sbjct: 327 SPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPP 386

Query: 361 ASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAALVE 420
           ASLQAIPY+ILIF VPLYET FVPLAR+LTG  SGISPLQR+GTGLF+ATFSMV+AALVE
Sbjct: 387 ASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVE 446

Query: 421 TKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMTYCS 480
            KRR S    +  LSI WIAPQF+IFG+SE+FTAVGLVEFFYKQS + MQSFLTAMTYCS
Sbjct: 447 KKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCS 506

Query: 481 YSFGFYLSSLLVSLVNKITRGSAAG---GWLSHNDLNNDRLDLFYWLLAGLSFVNFFNYL 540
           YSFGFYLSS+LVS VN++T  + +G   GWL  NDLN DRLD FYWLLA LSF+NFFNYL
Sbjct: 507 YSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYL 566

Query: 541 FWANRYS 545
           FW+  YS
Sbjct: 567 FWSRWYS 568

BLAST of MS018159 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 657.5 bits (1695), Expect = 9.2e-189
Identity = 348/544 (63.97%), Postives = 421/544 (77.39%), Query Frame = 0

Query: 1   VDWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVT 60
           VDWRGR  + N HGGM AA FVLGLQAFE+M IAAVGNNLITYV  +MHFPLSK+AN+VT
Sbjct: 30  VDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVT 89

Query: 61  NFVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAAT 120
           NFVGT+F+ +LLGG++SD++LGSF T+++FG VELSGF LL+VQAH P L+P  C+    
Sbjct: 90  NFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLID 149

Query: 121 STPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAY 180
            T C EA G +A +FF+ALYLVALGSGC+KPN+I+HGADQF +  PKQ K+LS++FN AY
Sbjct: 150 QT-CEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAY 209

Query: 181 FAFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTP 240
           FAF  GEL ALTLLVW+QTH+GMD+GFGVS AAM +G+I L+S T  +RN  PR +IFTP
Sbjct: 210 FAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTP 269

Query: 241 IAQVF---FNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDEG 300
           IA V         L+  S    L       +D           R  FLDKACIK + D  
Sbjct: 270 IAHVIVAAILKRKLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQ-DTN 329

Query: 301 REESPWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQ 360
            +ESPW+LCTV QVEQVK ++S++PIFA TI+FNTILAQLQTFSVQQG+SM++ L+  F 
Sbjct: 330 TKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFH 389

Query: 361 IPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAA 420
           IPPASLQAIPY++LIFLVPLY++  VP AR+LTG  SGI PL R+G GLF++TFSMVSAA
Sbjct: 390 IPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAA 449

Query: 421 LVETKRRNSSTAGSPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLTAMT 480
           ++E KRR+SS      LSI WI PQF+IFGISE+FTAVGL+EFFYKQS +GM+SFL A+T
Sbjct: 450 MLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALT 509

Query: 481 YCSYSFGFYLSSLLVSLVNKITRGSA-AGGWLSHNDLNNDRLDLFYWLLAGLSFVNFFNY 540
           YCSYSFGFY SS+LVS+VNKIT  S  + GWL  NDLN DRLDLFYWLLA LS +NF +Y
Sbjct: 510 YCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSY 569

BLAST of MS018159 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 393.3 bits (1009), Expect = 3.2e-109
Identity = 237/571 (41.51%), Postives = 332/571 (58.14%), Query Frame = 0

Query: 2   DWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVTN 61
           DWR RA     HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH   SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 62  FVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAATS 121
           F+GT FLL+LLGGF+SD++  +F+  L+   +E  G  +LT+QA  P L P  CD    S
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCD----S 134

Query: 122 TPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAYF 181
             C E SG +AAM FV LYLVALG G +K ++ SHGA+QF +  PK  K+ STFFN  YF
Sbjct: 135 PTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFN--YF 194

Query: 182 AFC--TGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFT 241
            FC   G L A+T +VWL+ + G + GFGVST A+ + ++  LS +  YRN  P G+  T
Sbjct: 195 VFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLT 254

Query: 242 PIAQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDE--- 301
            I +V    +     S S   +      S  N  + K   K+ +       K   +E   
Sbjct: 255 TILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSK--GKKEVESQGELEKPRQEEALP 314

Query: 302 --------------GREESPWKL---CTVAQVEQVKIILSVIPIFACTIIFNTILAQLQT 361
                           +E P      CTV QVE VKI+L ++PIFACTI+ N  LAQL T
Sbjct: 315 PRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLST 374

Query: 362 FSVQQGTSMDSWLTPKFQIPPASLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPL 421
           FSVQQ  SM++ +    +IPPASL   P + ++ L P+Y+   +P AR+ T   +G++ L
Sbjct: 375 FSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHL 434

Query: 422 QRVGTGLFVATFSMVSAALVETKRRNSS-------TAGSPPLSILWIAPQFVIFGISELF 481
           QR+G GL ++  +M  AALVE KR+  +       +  + P++ LWIA Q++  G ++LF
Sbjct: 435 QRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLF 494

Query: 482 TAVGLVEFFYKQSVEGMQSFLTAMTYCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHND 541
           T  GL+E+F+ ++   M+S  T++++ S + G+YLSS++VS+VN IT  S    WL    
Sbjct: 495 TLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKS 554

Query: 542 LNNDRLDLFYWLLAGLSFVNFFNYLFWANRY 544
           +N  +LD FYWL+  LS  NF +YLFWA RY
Sbjct: 555 INRYKLDYFYWLMCVLSAANFLHYLFWAMRY 576

BLAST of MS018159 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.8e-107
Identity = 219/549 (39.89%), Postives = 330/549 (60.11%), Query Frame = 0

Query: 2   DWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVTN 61
           DWR +A     HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH  L++S++ VT 
Sbjct: 13  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 72

Query: 62  FVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAATS 121
           F+ T FLL+LLGGF++D++  +F   L+   +E  G  LLT+QA  P L P PC  +A +
Sbjct: 73  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSA-A 132

Query: 122 TPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAYF 181
             C    G +AA  FV LYLV+LG G +K ++ SHGA+QF +  PK  K+ STFFN   F
Sbjct: 133 LRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVF 192

Query: 182 AFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTPI 241
               G L A+T +VW++ + G + GFGVST ++ L ++  L  +  Y+N  PRG+  T I
Sbjct: 193 CLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTI 252

Query: 242 AQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDEGREES 301
            +V    + +S  S +    +       ++    + +  + L     C+ ++  EG+   
Sbjct: 253 FKVLLAASIVSCSSKTSSNHFTSREVQSEHE---EKTPSQSLTNSLTCL-NKAIEGKTHH 312

Query: 302 PWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQIPPA 361
            W  CTV QVE VKI+L ++PIF CTI+ N  LAQL T+SV Q  +M+  +   F +P A
Sbjct: 313 IWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV-NFNVPSA 372

Query: 362 SLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAALVET 421
           SL   P + ++ L P Y+   +P AR++T +  GI+ LQR+G GL ++  +M  AALVE 
Sbjct: 373 SLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVEL 432

Query: 422 KRRN-SSTAG------SPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLT 481
           KR+  +  AG      + P++ LWIA Q++  G ++LFT  GL+EFF+ ++   M+S  T
Sbjct: 433 KRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLAT 492

Query: 482 AMTYCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFF 541
           ++++ S + G+YLSS++V +VN++T+ +    WL    LN +RLDLFYWL+  LS VNF 
Sbjct: 493 SLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGEK-LNRNRLDLFYWLMCVLSVVNFL 552

Query: 542 NYLFWANRY 544
           +YLFWA RY
Sbjct: 553 HYLFWAKRY 554

BLAST of MS018159 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.8e-107
Identity = 219/549 (39.89%), Postives = 330/549 (60.11%), Query Frame = 0

Query: 2   DWRGRACSSNYHGGMAAAAFVLGLQAFEMMAIAAVGNNLITYVFTQMHFPLSKSANVVTN 61
           DWR +A     HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH  L++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 62  FVGTVFLLSLLGGFVSDSYLGSFRTMLVFGLVELSGFTLLTVQAHYPGLRPAPCDMAATS 121
           F+ T FLL+LLGGF++D++  +F   L+   +E  G  LLT+QA  P L P PC  +A +
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSA-A 136

Query: 122 TPCVEASGCEAAMFFVALYLVALGSGCLKPNIISHGADQFPKEDPKQFKKLSTFFNCAYF 181
             C    G +AA  FV LYLV+LG G +K ++ SHGA+QF +  PK  K+ STFFN   F
Sbjct: 137 LRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVF 196

Query: 182 AFCTGELAALTLLVWLQTHAGMDVGFGVSTAAMLLGLICLLSATSLYRNTPPRGTIFTPI 241
               G L A+T +VW++ + G + GFGVST ++ L ++  L  +  Y+N  PRG+  T I
Sbjct: 197 CLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTI 256

Query: 242 AQVFFNHASLSLHSLSLFLSYLWDRFSDQNSFLMKYSCKRRLFLDKACIKSEDDEGREES 301
            +V    + +S  S +    +       ++    + +  + L     C+ ++  EG+   
Sbjct: 257 FKVLLAASIVSCSSKTSSNHFTSREVQSEHE---EKTPSQSLTNSLTCL-NKAIEGKTHH 316

Query: 302 PWKLCTVAQVEQVKIILSVIPIFACTIIFNTILAQLQTFSVQQGTSMDSWLTPKFQIPPA 361
            W  CTV QVE VKI+L ++PIF CTI+ N  LAQL T+SV Q  +M+  +   F +P A
Sbjct: 317 IWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV-NFNVPSA 376

Query: 362 SLQAIPYLILIFLVPLYETAFVPLARRLTGAPSGISPLQRVGTGLFVATFSMVSAALVET 421
           SL   P + ++ L P Y+   +P AR++T +  GI+ LQR+G GL ++  +M  AALVE 
Sbjct: 377 SLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVEL 436

Query: 422 KRRN-SSTAG------SPPLSILWIAPQFVIFGISELFTAVGLVEFFYKQSVEGMQSFLT 481
           KR+  +  AG      + P++ LWIA Q++  G ++LFT  GL+EFF+ ++   M+S  T
Sbjct: 437 KRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLAT 496

Query: 482 AMTYCSYSFGFYLSSLLVSLVNKITRGSAAGGWLSHNDLNNDRLDLFYWLLAGLSFVNFF 541
           ++++ S + G+YLSS++V +VN++T+ +    WL    LN +RLDLFYWL+  LS VNF 
Sbjct: 497 SLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGEK-LNRNRLDLFYWLMCVLSVVNFL 556

Query: 542 NYLFWANRY 544
           +YLFWA RY
Sbjct: 557 HYLFWAKRY 558