MS017563.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS017563.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold373: 612801 .. 614899 (+)
Sequence length	1368
RNA-Seq Expression	MS017563.1
Synteny	MS017563.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAAAACAGGTGTGTTTGATTCTGATCCCACGGTCATGGGGAAGGCGACGGAGTTGAAGAAGGAGTTACAGAGACTCATTCTGGCTATAATAGACGACGACAATTACTCCACCGACACCATCGATCACGCTAAAGAGATTCTATGTGCCCTCAAGGAGTTGAAACTCCGGAAGAGATCGCGCTCTTCTAATCTTTACGAGAACCTCATCTGCCCTGATCAATTCCGCTGTCCGCTCTCCAGAGAACTCATGAGAGATCCTGTTGTTGTATCTACTGGCGAGGTTTGTTTCATGGCTCTCTGCTTTCTAGGTTATGTTGAAAGAAATTTGCGCGTTTCTTGATTACTTTTTGTTTTTTCTTATAAAAACAAACTAATGTGTTTGCGGTGTTCTTGTTTCGCGTTTCAGTTCGGGGGGTTTTGTTTGTTTCCCCCCTTCGCGTTGAAATCCTGTGTTTGTGGATCAAAACACGAAGCGCATAGAGAAACGATAATTTGAGAACCTGAGTGTGTTCTTCTTCTCACCTTTCCTTCGTCTCTGATTTTATAGTCCTGTAAATCTGCTGTTTCGGAGTGTCTTCTTCTTTTATTCTTCTTTAAATTACACCGTCTTCTATTTGGTGGACAAGAGGTTATTTCGAAAATAAAGTACTTTCGAATTCGATATTTACAGTCTATTGGATTTTCAGGTTTCTGTGCTTTTTGGATTTGGGTTTCCAATTTGATGATTTCTGTTTTGTGACTGTATTATCACTGACATTGCATCTTTGTATTTAATTTTCGAGTTGCTGTATTCGTGTTTCGTGTTGTATTTGAATTGAATGCAAGGTAACGAATATTGACTGTGAATAAATATGGATGATTTTCCTGGAAATCTGTATAATCATTTGTTATATGTATAATTTTGGTGGACTAGATACTAATACCATTATGAATCCTTTGTTACCTCAAGACATGCATTATGGTCGTTTCTGAGTTTTATAATTGTTACATTTTATTTTTATCTTATTGCAGACATACGACAGACCCTTTATCCACAAATGGTTAAAAGCAGGAAACCGGACGTGCCCCAGAACTCAGCAAGTTCTTTCACACACTAACCTCACTCCCAATCACTTGATTCGTGAAATGATTTCTCAATGGTGTAAAAGTCGGGGAATCGAGCTGCAAGATCGTGTTCATTATCTTGATGACGACACGATAACGGAGGCTGATAGGGATCAGCTTCTTACGTTAATAGAGAAAATGTCTATGACAATATCTGAACAGAAAGCAGCTGCAAAGGAGCTTCGCATGTTGACAAAGCGGATGCCCTCATTTCGAGCACTTTTTGGTGAGTCCCTTGATGCGATCTCTTCGCTGCTTAGTCCACTTTGTGGAGATAATTCACAAAGCAGTATTCACACCGACCTGCAAGAAGATGTGATTACGACCCTCCTGAATCTGTCAATCCATGACAACAACAAAAAGCTTGTTGCTGAAACTCCCAAGGTAATACCTCTTCTCATGGAGGCGTTGAGATCGGGAACCATGGAAACGAGGAGCAATGCTGCTGCAGCCCTTTTTACTTTGTCTGCTCTCGACTCAAACAAAGCACTTATCGGGAAATCTGGTGCCTTGGAACCGCTTATCGACCTTTTAGATGAGGGACATCCATTAGCTATCAAAGATGCTGCTTCAGCAATATTCAATCTTTGTATAATTCATGAAAACAAAGCAAGAGCTGTGCGAGATGGTGCGGTTAGAGTGCTTCTAAAAAAGATCATGAAGCAAATGCATGTTGATGAATTGTTGGCTATTCTTGCAATGCTTTCAAACCATCCAAAAGCTATCGAAGAAATGGGAGAACTTGGAGCTGTTCCTTTCTTGCTCAGAATTATCAGAGAAAGTCCTTGTTCACGTAACAAGGAGAATTGCATCGTGATCATCCATGCTATCTGCTTATATGATCGCACGAAGTGGAAGGACATAAGGGAGGAGGAGAAAAGCTATCGAACTATCTCCGAACTTGCTCAGAATGGGACTTCAAGGGCCAAGAGAAAGGCCAGTGGCATACTTGAGAGACTGAACAGGGCAGTTAATATGACCCATACTGCA

mRNA sequence

ATGGCGAAAACAGGTGTGTTTGATTCTGATCCCACGGTCATGGGGAAGGCGACGGAGTTGAAGAAGGAGTTACAGAGACTCATTCTGGCTATAATAGACGACGACAATTACTCCACCGACACCATCGATCACGCTAAAGAGATTCTATGTGCCCTCAAGGAGTTGAAACTCCGGAAGAGATCGCGCTCTTCTAATCTTTACGAGAACCTCATCTGCCCTGATCAATTCCGCTGTCCGCTCTCCAGAGAACTCATGAGAGATCCTGTTGTTGTATCTACTGGCGAGACATACGACAGACCCTTTATCCACAAATGGTTAAAAGCAGGAAACCGGACGTGCCCCAGAACTCAGCAAGTTCTTTCACACACTAACCTCACTCCCAATCACTTGATTCGTGAAATGATTTCTCAATGGTGTAAAAGTCGGGGAATCGAGCTGCAAGATCGTGTTCATTATCTTGATGACGACACGATAACGGAGGCTGATAGGGATCAGCTTCTTACGTTAATAGAGAAAATGTCTATGACAATATCTGAACAGAAAGCAGCTGCAAAGGAGCTTCGCATGTTGACAAAGCGGATGCCCTCATTTCGAGCACTTTTTGGTGAGTCCCTTGATGCGATCTCTTCGCTGCTTAGTCCACTTTGTGGAGATAATTCACAAAGCAGTATTCACACCGACCTGCAAGAAGATGTGATTACGACCCTCCTGAATCTGTCAATCCATGACAACAACAAAAAGCTTGTTGCTGAAACTCCCAAGGTAATACCTCTTCTCATGGAGGCGTTGAGATCGGGAACCATGGAAACGAGGAGCAATGCTGCTGCAGCCCTTTTTACTTTGTCTGCTCTCGACTCAAACAAAGCACTTATCGGGAAATCTGGTGCCTTGGAACCGCTTATCGACCTTTTAGATGAGGGACATCCATTAGCTATCAAAGATGCTGCTTCAGCAATATTCAATCTTTGTATAATTCATGAAAACAAAGCAAGAGCTGTGCGAGATGGTGCGGTTAGAGTGCTTCTAAAAAAGATCATGAAGCAAATGCATGTTGATGAATTGTTGGCTATTCTTGCAATGCTTTCAAACCATCCAAAAGCTATCGAAGAAATGGGAGAACTTGGAGCTGTTCCTTTCTTGCTCAGAATTATCAGAGAAAGTCCTTGTTCACGTAACAAGGAGAATTGCATCGTGATCATCCATGCTATCTGCTTATATGATCGCACGAAGTGGAAGGACATAAGGGAGGAGGAGAAAAGCTATCGAACTATCTCCGAACTTGCTCAGAATGGGACTTCAAGGGCCAAGAGAAAGGCCAGTGGCATACTTGAGAGACTGAACAGGGCAGTTAATATGACCCATACTGCA

Coding sequence (CDS)

ATGGCGAAAACAGGTGTGTTTGATTCTGATCCCACGGTCATGGGGAAGGCGACGGAGTTGAAGAAGGAGTTACAGAGACTCATTCTGGCTATAATAGACGACGACAATTACTCCACCGACACCATCGATCACGCTAAAGAGATTCTATGTGCCCTCAAGGAGTTGAAACTCCGGAAGAGATCGCGCTCTTCTAATCTTTACGAGAACCTCATCTGCCCTGATCAATTCCGCTGTCCGCTCTCCAGAGAACTCATGAGAGATCCTGTTGTTGTATCTACTGGCGAGACATACGACAGACCCTTTATCCACAAATGGTTAAAAGCAGGAAACCGGACGTGCCCCAGAACTCAGCAAGTTCTTTCACACACTAACCTCACTCCCAATCACTTGATTCGTGAAATGATTTCTCAATGGTGTAAAAGTCGGGGAATCGAGCTGCAAGATCGTGTTCATTATCTTGATGACGACACGATAACGGAGGCTGATAGGGATCAGCTTCTTACGTTAATAGAGAAAATGTCTATGACAATATCTGAACAGAAAGCAGCTGCAAAGGAGCTTCGCATGTTGACAAAGCGGATGCCCTCATTTCGAGCACTTTTTGGTGAGTCCCTTGATGCGATCTCTTCGCTGCTTAGTCCACTTTGTGGAGATAATTCACAAAGCAGTATTCACACCGACCTGCAAGAAGATGTGATTACGACCCTCCTGAATCTGTCAATCCATGACAACAACAAAAAGCTTGTTGCTGAAACTCCCAAGGTAATACCTCTTCTCATGGAGGCGTTGAGATCGGGAACCATGGAAACGAGGAGCAATGCTGCTGCAGCCCTTTTTACTTTGTCTGCTCTCGACTCAAACAAAGCACTTATCGGGAAATCTGGTGCCTTGGAACCGCTTATCGACCTTTTAGATGAGGGACATCCATTAGCTATCAAAGATGCTGCTTCAGCAATATTCAATCTTTGTATAATTCATGAAAACAAAGCAAGAGCTGTGCGAGATGGTGCGGTTAGAGTGCTTCTAAAAAAGATCATGAAGCAAATGCATGTTGATGAATTGTTGGCTATTCTTGCAATGCTTTCAAACCATCCAAAAGCTATCGAAGAAATGGGAGAACTTGGAGCTGTTCCTTTCTTGCTCAGAATTATCAGAGAAAGTCCTTGTTCACGTAACAAGGAGAATTGCATCGTGATCATCCATGCTATCTGCTTATATGATCGCACGAAGTGGAAGGACATAAGGGAGGAGGAGAAAAGCTATCGAACTATCTCCGAACTTGCTCAGAATGGGACTTCAAGGGCCAAGAGAAAGGCCAGTGGCATACTTGAGAGACTGAACAGGGCAGTTAATATGACCCATACTGCA

Protein sequence

MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKRSRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVLSHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQLLTLIEKMSMTISEQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA

Homology

[Close]

BLAST of MS017563.1 vs. NCBI nr
Match: XP_022159455.1 (U-box domain-containing protein 9 [Momordica charantia])

HSP 1 Score: 887.5 bits (2292), Expect = 4.9e-254
Identity = 455/456 (99.78%), Postives = 455/456 (99.78%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60

Query: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120
           SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL
Sbjct: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQLLTLIEKMSMTISEQ 180
           SHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQ LTLIEKMSMTISEQ
Sbjct: 121 SHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQFLTLIEKMSMTISEQ 180

Query: 181 KAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLS 240
           KAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLS
Sbjct: 181 KAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLS 240

Query: 241 IHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPL 300
           IHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPL
Sbjct: 241 IHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPL 300

Query: 301 IDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAM 360
           IDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAM
Sbjct: 301 IDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAM 360

Query: 361 LSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKS 420
           LSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKS
Sbjct: 361 LSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKS 420

Query: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 456

[Close]

BLAST of MS017563.1 vs. NCBI nr
Match: XP_022967253.1 (U-box domain-containing protein 9-like [Cucurbita maxima])

HSP 1 Score: 813.9 bits (2101), Expect = 6.9e-232
Identity = 419/458 (91.48%), Postives = 436/458 (95.20%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSDPTVMGKATELK+ELQRLI AIIDDDN STDTIDHAKEI+ ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120
           SRSSN YENLICPD+FRCPLSRELMRDPVVVSTGETYDRPFIHKWL++GNRTCPRTQQVL
Sbjct: 61  SRSSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCKSRGIELQD--RVHYLDDDTITEADRDQLLTLIEKMSMTIS 180
           SHTNLTPNHLIRE+ISQWC SRGIELQD  RVH LDDD ITEADRD+ LTLIE MSMTIS
Sbjct: 121 SHTNLTPNHLIREIISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTIS 180

Query: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLN 240
           EQKAAAKELRMLTKRMPSFRALFGESLDAIS LLSPLCGD SQSS+H DLQEDVITTLLN
Sbjct: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSVHADLQEDVITTLLN 240

Query: 241 LSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALE 300
           LSIHDNNKKLVAETP VIPLL EALRSGTMETRSNAAAALFTLSALDSNKALIGKSGAL+
Sbjct: 241 LSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALK 300

Query: 301 PLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAIL 360
           PLIDLLDEGHPL+IKDAASAIFNLCI+HENKARAVRDGAVRVLLKKIM QMHVDELLAIL
Sbjct: 301 PLIDLLDEGHPLSIKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 360

Query: 361 AMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEE 420
           AMLS+H KA+EE+GELGAVP L RIIRES CSRNKENCIVIIHAICLYDRTKWKD++EEE
Sbjct: 361 AMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKEEE 420

Query: 421 KSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           K YRTISELA+NGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 KWYRTISELAKNGTSRAKRKASGILERLNRAVNMTHTA 458

[Close]

BLAST of MS017563.1 vs. NCBI nr
Match: XP_038888241.1 (U-box domain-containing protein 9 [Benincasa hispida])

HSP 1 Score: 813.9 bits (2101), Expect = 6.9e-232
Identity = 423/460 (91.96%), Postives = 440/460 (95.65%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSDPTVMGKATELK+ELQRLI+AIIDDDNYSTDTID+AKEIL ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60

Query: 61  SR-SSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQV 120
           SR SSNL+ENLICP++FRCPLSRELMRDPV+VSTGETYD+PFI KWLK+GNRTCPRTQQV
Sbjct: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDKPFIQKWLKSGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCKSRGIELQD--RVHYLDDDTITEADRDQLLTLIEKMSMTI 180
           LSHTNLTPNHLIREMISQWC SRGIELQD  RVHYLDDD ITEADRD+ LTLIEKMSMTI
Sbjct: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180

Query: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQ-SSIHTDLQEDVITTL 240
           SEQKAAAKELRMLTKRMPSFRALFGESLDAIS LLSPLCGD SQ SSIHTDLQEDVITTL
Sbjct: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSSIHTDLQEDVITTL 240

Query: 241 LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGA 300
           LNLSIHDNNKKLV ETP VIPLLMEAL SGTMETRSNAAAALFTLSALDSNKA+IGK+GA
Sbjct: 241 LNLSIHDNNKKLVIETPNVIPLLMEALGSGTMETRSNAAAALFTLSALDSNKAIIGKAGA 300

Query: 301 LEPLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLA 360
           L+PLI LLDEGHPL+IKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIM QMHVDELLA
Sbjct: 301 LKPLIKLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360

Query: 361 ILAMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIRE 420
           ILAMLS+H KAIEE+GELGAVPFLLRIIRES CSRNKENCIVIIHAICLYDRTKWKD+RE
Sbjct: 361 ILAMLSSHQKAIEEIGELGAVPFLLRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMRE 420

Query: 421 EEKSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           EEK YRTISELAQNGTSRAKRKASGILERLNR VNMTHTA
Sbjct: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 460

[Close]

BLAST of MS017563.1 vs. NCBI nr
Match: XP_022963574.1 (U-box domain-containing protein 9-like [Cucurbita moschata])

HSP 1 Score: 813.9 bits (2101), Expect = 6.9e-232
Identity = 419/458 (91.48%), Postives = 435/458 (94.98%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSDPTVMGKATELK+ELQRLI AIIDDDN STDTIDHAKEI+ ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120
           SRSSN YENLICPD+FRCPLSRELMRDPVVVSTGETYDRPFIHKWL++GNRTCPRTQQVL
Sbjct: 61  SRSSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCKSRGIELQD--RVHYLDDDTITEADRDQLLTLIEKMSMTIS 180
           SHTNLTPNHLIREMISQWC SRGIELQD  RVH LDDD ITEADRD+ LTLIE MSMTI 
Sbjct: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTIP 180

Query: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLN 240
           EQKAAAKELRMLTKRMPSFRALFGESLDAIS LLSPLCGD SQSS+H DLQEDVITTLLN
Sbjct: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSVHADLQEDVITTLLN 240

Query: 241 LSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALE 300
           LSIHDNNKKLVAETP VIPLL EALRSGTMETRSNAAAALFTLSALDSNKALIGKSGAL+
Sbjct: 241 LSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALK 300

Query: 301 PLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAIL 360
           PLIDLLDEGHPL++KDAASAIFNLCI+HENKARAVRDGAVRVLLKKIM QMHVDELLAIL
Sbjct: 301 PLIDLLDEGHPLSMKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 360

Query: 361 AMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEE 420
           AMLS+H KA+EE+GELGAVP L RIIRES CSRNKENCIVIIHAICLYDRTKWKD++EEE
Sbjct: 361 AMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKEEE 420

Query: 421 KSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           K YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 KWYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 458

[Close]

BLAST of MS017563.1 vs. NCBI nr
Match: XP_023554208.1 (U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 812.8 bits (2098), Expect = 1.5e-231
Identity = 419/458 (91.48%), Postives = 435/458 (94.98%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSDPTVMGKATELK+ELQRLI AIIDDDN STDTIDHAKEI+ ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120
           SRSSN YENLICPD+FRCPLSRELMRDPVVVSTGETYDRPFIHKWL++GNRTCPRTQQVL
Sbjct: 61  SRSSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCKSRGIELQD--RVHYLDDDTITEADRDQLLTLIEKMSMTIS 180
           SHTNLTPNHLIREMISQWC SRGIELQD  RVH L+DD ITEADRD+ LTLIE MSMTI 
Sbjct: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVRVHCLEDDVITEADRDRFLTLIENMSMTIP 180

Query: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLN 240
           EQKAAAKELRMLTKRMPSFRALFGESLDAIS LLSPLCGD SQSSIH DLQEDVITTLLN
Sbjct: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSIHADLQEDVITTLLN 240

Query: 241 LSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALE 300
           LSIHDNNKKLVAETP VIPLL EALRSGTMETRSNAAAALFTLSALDSNKALIGKSGAL+
Sbjct: 241 LSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALK 300

Query: 301 PLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAIL 360
           PLIDLLDEGHPL++KDAASAIFNLCI+HENKARAVRDGAVRVLLKKIM QMHVDELLAIL
Sbjct: 301 PLIDLLDEGHPLSMKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 360

Query: 361 AMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEE 420
           AMLS+H KA+EE+GELGAVP L RIIRES CSRNKENCIVIIHAICLYDRTKWKD++EEE
Sbjct: 361 AMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKEEE 420

Query: 421 KSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           K YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 KWYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 458

[Close]

BLAST of MS017563.1 vs. ExPASy Swiss-Prot
Match: Q9SRT0 (U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 1.7e-156
Identity = 288/464 (62.07%), Postives = 370/464 (79.74%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSDPT + KA ELK+E+++L++ I D+D+    TID  ++ L AL+E  +RK 
Sbjct: 1   MAKTGVFDSDPTAIAKAKELKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKM 60

Query: 61  SRSSNL--YENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQ 120
           ++SS+L   E + CP++FRCPLS ELMRDPVV+++G+TYD+ FI KWL +GNRTCP+TQQ
Sbjct: 61  AKSSSLEMLETVSCPEEFRCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQ 120

Query: 121 VLSHTNLTPNHLIREMISQWCKSRGIELQDRVH---YLDDDTITEADRDQLLTLIEKMSM 180
           VL HT LTPN LIREMIS+WCK  G+E + + H     +D+T+T +DR+   +L+ K+S 
Sbjct: 121 VLPHTALTPNLLIREMISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSS 180

Query: 181 T-ISEQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLC-GDNSQSSIHTDLQEDVI 240
           + + +QK+AAKELR+LT++   FRALFGES D I+ L++PL  G N        LQEDV+
Sbjct: 181 SNLQDQKSAAKELRLLTRKGTEFRALFGESPDEITRLVNPLLHGSNPDEK----LQEDVV 240

Query: 241 TTLLNLSIH-DNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIG 300
           TTLLN+SIH D+NKKLV E P VIPLL++ALR GT+ TRSNAAAA+FTLSALDSNK LIG
Sbjct: 241 TTLLNISIHDDSNKKLVCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIG 300

Query: 301 KSGALEPLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVD 360
           KSG L+PLIDLL+EG+PLAIKD A+AIF LCI HEN++RAVRDGAVRVL KKI   ++VD
Sbjct: 301 KSGILKPLIDLLEEGNPLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVD 360

Query: 361 ELLAILAMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWK 420
           ELLAILAML  H KA+EE+GELG V +LL+I RES C RNKEN IVI+H IC  DRTKWK
Sbjct: 361 ELLAILAMLVTHWKAVEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWK 420

Query: 421 DIREEEKSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           +I+EEE ++ TI++L++ GTSRA+RKA+GIL+RL +A+N+THTA
Sbjct: 421 EIKEEENAHGTITKLSREGTSRAQRKANGILDRLRKAMNLTHTA 460

[Close]

BLAST of MS017563.1 vs. ExPASy Swiss-Prot
Match: Q3E9F7 (Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=PUB46 PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 8.3e-79
Identity = 180/450 (40.00%), Postives = 276/450 (61.33%), Query Frame = 0


Query: 17  ATELKKELQRLILAIIDD----DNYSTD-------TIDHAKEILCALKELKLRKRSRSSN 76
           A  L++ELQ+++  I++D    D   T+        ID A  IL  L++++ +      +
Sbjct: 9   ADTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLRKVESKIPESDIS 68

Query: 77  LYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVLSHTNL 136
             E    P +F C LS  +M +PV++++G+TY++ +I +WLK   RTCP+T+QVLSH   
Sbjct: 69  PVE---VPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWLK-HERTCPKTKQVLSHRLW 128

Query: 137 TPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQLLTLIEKM--SMTISEQKAA 196
            PNHLI ++I+QWC     + Q       D+ + E     +  L++++  S ++++Q  A
Sbjct: 129 IPNHLISDLITQWCLVNKYDHQKP----SDELVAELFTSDIEALLQRVSSSSSVADQIEA 188

Query: 197 AKELRMLTKRMPSFRALFGESL-DAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLSIH 256
           AKELR  TK+ P+ R  F   + D+I+ LLSPL   +       +LQE+++T L NLSI 
Sbjct: 189 AKELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLSIL 248

Query: 257 DNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPLID 316
           ++NK ++AE   VIPLL ++L+ GT ETR NAAA L +LSA+DSNK +IG S A++ LID
Sbjct: 249 ESNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKALID 308

Query: 317 LLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAMLS 376
           L++EG  LA K+A S +FNLCI+ ENK + V  G +    KKI    +VDELL++LA++S
Sbjct: 309 LIEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKIKAGSNVDELLSLLALIS 368

Query: 377 NHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAIC--LYDRTKWKDIREEEKS 436
            H +A+EEM +LG +  L  I+R+       EN +VI+  +     DR++ K + EEE  
Sbjct: 369 THNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEEENQ 428

Query: 437 YRTISELAQNGTSRAKRKASGILERLNRAV 451
           + T ++LA+ G+ RA RKA GIL+ + R V
Sbjct: 429 HGTFTKLAKQGSVRAARKAQGILQWIKRFV 450

[Close]

BLAST of MS017563.1 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.0e-73
Identity = 177/445 (39.78%), Postives = 271/445 (60.90%), Query Frame = 0


Query: 19  ELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRK-------RSRSSNL-YENL 78
           ++K E   L   +I         +D    +L  LK+  + +        +RS+++ + + 
Sbjct: 167 DMKNESIALHNMVISTAGEPDGCVDQMSSLLKKLKDCVVTEDHANDALTTRSASIKHRSP 226

Query: 79  ICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVLSHTNLTPNHL 138
           I PD+FRCP+S ELM+DPV+VS+G+TY+R  I KWL +G++TCP+TQQ LSHT+LTPN +
Sbjct: 227 IIPDEFRCPISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLSHTSLTPNFV 286

Query: 139 IREMISQWCKSRGIEL-QDRVHYLDDDTITEADRDQ--LLTLIEKM-SMTISEQKAAAKE 198
           ++ +ISQWC++ GIEL +++ +  D      +D D   L++L+ ++ S    EQ+AAA E
Sbjct: 287 LKSLISQWCEANGIELPKNKQNSRDKKAAKSSDYDHAGLVSLMNRLRSGNQDEQRAAAGE 346

Query: 199 LRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLSIHDNNK 258
           +R+L KR  + R    E+  AI  L+      N  SS     QE  +T LLNLSIH+NNK
Sbjct: 347 IRLLAKRNVNNRICIAEA-GAIPLLV------NLLSSSDPRTQEHAVTALLNLSIHENNK 406

Query: 259 KLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPLIDLLDE 318
             + ++   IP ++E L++G+METR NAAA LF+LS +D NK  IG +GA+ PLI+LL +
Sbjct: 407 ASIVDS-HAIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPPLINLLCD 466

Query: 319 GHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQM--HVDELLAILAMLSNH 378
           G P   KDAA+AIFNLCI   NK RAV+ G V  L+  ++      +DE L++L++L+ +
Sbjct: 467 GSPRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSLLSILAGN 526

Query: 379 PKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKSYRTI 438
           P+    +     +P L+ +I+ +   RN+EN   I+  +C  D T+     +       +
Sbjct: 527 PEGKIVIARSEPIPPLVEVIK-TGSPRNRENAAAILWLLCSAD-TEQTLAAKAAGVEDAL 586

Query: 439 SELAQNGTSRAKRKASGILERLNRA 450
            EL++ GT RAKRKAS ILE +++A
Sbjct: 587 KELSETGTDRAKRKASSILELMHQA 601

[Close]

BLAST of MS017563.1 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 8.9e-73
Identity = 181/475 (38.11%), Postives = 268/475 (56.42%), Query Frame = 0


Query: 6   VFDSDPTVMGKAT---------ELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELK 65
           V D DP ++ + +         ELKKE   +    +  D    D  +    +L  L +  
Sbjct: 165 VMDPDPIILKRLSQELQLTTIDELKKESHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFV 224

Query: 66  LRKR--------SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKA 125
             +         SR  + + + + P+ FRCP+S ELM+DPV+VSTG+TY+R  I KWL A
Sbjct: 225 TMESSDPDPSTGSRIVSRHRSPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDA 284

Query: 126 GNRTCPRTQQVLSHTNLTPNHLIREMISQWCKSRGIELQD-----RVHYLDDDTITEADR 185
           G++TCP++Q+ L H  LTPN++++ +I+ WC+S GIEL       R   +   + ++ DR
Sbjct: 285 GHKTCPKSQETLLHAGLTPNYVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSSSDCDR 344

Query: 186 DQLLTLIEKMSM-TISEQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQS 245
             +L+L+EK++  T  +Q+AAA ELR+L KR    R    E+  AI  L+  L      S
Sbjct: 345 TFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEA-GAIPLLVELL------S 404

Query: 246 SIHTDLQEDVITTLLNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLS 305
           S     QE  +T LLNLSI++ NK  + +   +   ++E L++G+ME R NAAA LF+LS
Sbjct: 405 SPDPRTQEHSVTALLNLSINEGNKGAIVDAGAITD-IVEVLKNGSMEARENAAATLFSLS 464

Query: 306 ALDSNKALIGKSGALEPLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVL- 365
            +D NK  IG +GA++ LI LL+EG     KDAA+AIFNLCI   NK+RAV+ G V  L 
Sbjct: 465 VIDENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLT 524

Query: 366 -LKKIMKQMHVDELLAILAMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVII 425
            L K      VDE LAILA+LS + +    + E  ++P L+ IIR +   RN+EN   I+
Sbjct: 525 RLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEIIR-TGSPRNRENAAAIL 584

Query: 426 HAICLYDRTKWKDIREEEKSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHT 456
             +C+ +  +    RE       + EL +NGT RAKRKA+ +LE + +   +  T
Sbjct: 585 WYLCIGNIERLNVAREVGADV-ALKELTENGTDRAKRKAASLLELIQQTEGVAVT 629

[Close]

BLAST of MS017563.1 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 263.1 bits (671), Expect = 5.9e-69
Identity = 159/397 (40.05%), Postives = 239/397 (60.20%), Query Frame = 0


Query: 57  LRKRSRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRT 116
           + K +  S   + L  P  F CP+S ELM+DPV+V+TG+TY+R +I +W+  GN TCP+T
Sbjct: 226 VNKNTDESKKSDKLTIPVDFLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCPKT 285

Query: 117 QQVLSHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQLLTLIEKMSMT 176
           QQ L +  LTPN+++R +IS+WC    IE      Y++  T    D   +  L++++S  
Sbjct: 286 QQKLENFTLTPNYVLRSLISRWCAEHNIE--QPAGYINGRTKNSGDMSVIRALVQRLSSR 345

Query: 177 ISE-QKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITT 236
            +E ++ A  E+R L+KR    R L  E+  AI  L++ L  ++  +      QE+ IT 
Sbjct: 346 STEDRRNAVSEIRSLSKRSTDNRILIAEA-GAIPVLVNLLTSEDVAT------QENAITC 405

Query: 237 LLNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSG 296
           +LNLSI++NNK+L+     V   +++ LR+GTME R NAAA LF+LS  D NK +IG SG
Sbjct: 406 VLNLSIYENNKELIMFAGAVTS-IVQVLRAGTMEARENAAATLFSLSLADENKIIIGGSG 465

Query: 297 ALEPLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKI---MKQMHVD 356
           A+  L+DLL+ G P   KDAA+A+FNLCI H NK RAVR G V  L+K +    +   VD
Sbjct: 466 AIPALVDLLENGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDSTRHRMVD 525

Query: 357 ELLAILAMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWK 416
           E L IL++L+N+  A   + +   +P L+ I+ ++  +RN+EN   I+ ++C  D  K  
Sbjct: 526 EALTILSVLANNQDAKSAIVKANTLPALIGIL-QTDQTRNRENAAAILLSLCKRDTEKLI 585

Query: 417 DIREEEKSYRTISELAQNGTSRAKRKASGILERLNRA 450
            I     +   + +L++NGT R KRKA  +LE L +A
Sbjct: 586 TI-GRLGAVVPLMDLSKNGTERGKRKAISLLELLRKA 610

[Close]

BLAST of MS017563.1 vs. ExPASy TrEMBL
Match: A0A6J1DYS9 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111025869 PE=4 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.4e-254
Identity = 455/456 (99.78%), Postives = 455/456 (99.78%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60

Query: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120
           SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL
Sbjct: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQLLTLIEKMSMTISEQ 180
           SHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQ LTLIEKMSMTISEQ
Sbjct: 121 SHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQFLTLIEKMSMTISEQ 180

Query: 181 KAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLS 240
           KAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLS
Sbjct: 181 KAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLS 240

Query: 241 IHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPL 300
           IHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPL
Sbjct: 241 IHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPL 300

Query: 301 IDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAM 360
           IDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAM
Sbjct: 301 IDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAM 360

Query: 361 LSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKS 420
           LSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKS
Sbjct: 361 LSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKS 420

Query: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 456

[Close]

BLAST of MS017563.1 vs. ExPASy TrEMBL
Match: A0A6J1HUJ6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466836 PE=4 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 3.3e-232
Identity = 419/458 (91.48%), Postives = 436/458 (95.20%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSDPTVMGKATELK+ELQRLI AIIDDDN STDTIDHAKEI+ ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120
           SRSSN YENLICPD+FRCPLSRELMRDPVVVSTGETYDRPFIHKWL++GNRTCPRTQQVL
Sbjct: 61  SRSSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCKSRGIELQD--RVHYLDDDTITEADRDQLLTLIEKMSMTIS 180
           SHTNLTPNHLIRE+ISQWC SRGIELQD  RVH LDDD ITEADRD+ LTLIE MSMTIS
Sbjct: 121 SHTNLTPNHLIREIISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTIS 180

Query: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLN 240
           EQKAAAKELRMLTKRMPSFRALFGESLDAIS LLSPLCGD SQSS+H DLQEDVITTLLN
Sbjct: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSVHADLQEDVITTLLN 240

Query: 241 LSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALE 300
           LSIHDNNKKLVAETP VIPLL EALRSGTMETRSNAAAALFTLSALDSNKALIGKSGAL+
Sbjct: 241 LSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALK 300

Query: 301 PLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAIL 360
           PLIDLLDEGHPL+IKDAASAIFNLCI+HENKARAVRDGAVRVLLKKIM QMHVDELLAIL
Sbjct: 301 PLIDLLDEGHPLSIKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 360

Query: 361 AMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEE 420
           AMLS+H KA+EE+GELGAVP L RIIRES CSRNKENCIVIIHAICLYDRTKWKD++EEE
Sbjct: 361 AMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKEEE 420

Query: 421 KSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           K YRTISELA+NGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 KWYRTISELAKNGTSRAKRKASGILERLNRAVNMTHTA 458

[Close]

BLAST of MS017563.1 vs. ExPASy TrEMBL
Match: A0A6J1HKK5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111463870 PE=4 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 3.3e-232
Identity = 419/458 (91.48%), Postives = 435/458 (94.98%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSDPTVMGKATELK+ELQRLI AIIDDDN STDTIDHAKEI+ ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120
           SRSSN YENLICPD+FRCPLSRELMRDPVVVSTGETYDRPFIHKWL++GNRTCPRTQQVL
Sbjct: 61  SRSSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCKSRGIELQD--RVHYLDDDTITEADRDQLLTLIEKMSMTIS 180
           SHTNLTPNHLIREMISQWC SRGIELQD  RVH LDDD ITEADRD+ LTLIE MSMTI 
Sbjct: 121 SHTNLTPNHLIREMISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTIP 180

Query: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLN 240
           EQKAAAKELRMLTKRMPSFRALFGESLDAIS LLSPLCGD SQSS+H DLQEDVITTLLN
Sbjct: 181 EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSVHADLQEDVITTLLN 240

Query: 241 LSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALE 300
           LSIHDNNKKLVAETP VIPLL EALRSGTMETRSNAAAALFTLSALDSNKALIGKSGAL+
Sbjct: 241 LSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALK 300

Query: 301 PLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAIL 360
           PLIDLLDEGHPL++KDAASAIFNLCI+HENKARAVRDGAVRVLLKKIM QMHVDELLAIL
Sbjct: 301 PLIDLLDEGHPLSMKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 360

Query: 361 AMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEE 420
           AMLS+H KA+EE+GELGAVP L RIIRES CSRNKENCIVIIHAICLYDRTKWKD++EEE
Sbjct: 361 AMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKEEE 420

Query: 421 KSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           K YRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA
Sbjct: 421 KWYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 458

[Close]

BLAST of MS017563.1 vs. ExPASy TrEMBL
Match: A0A6J1K749 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491272 PE=4 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 9.4e-227
Identity = 405/455 (89.01%), Postives = 428/455 (94.07%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PTVMGKA +LK+ELQ+LI+AIIDDDNYSTD ID AKEILCALKELKLRKR
Sbjct: 1   MAKTGVFDSNPTVMGKAMDLKRELQKLIVAIIDDDNYSTDAIDQAKEILCALKELKLRKR 60

Query: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120
           SRSSNLYENLICPD+FRCPLS ELMRDPVVVSTGET+DRPFI KWLK+GNRTCPRTQQVL
Sbjct: 61  SRSSNLYENLICPDEFRCPLSTELMRDPVVVSTGETFDRPFIQKWLKSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQLLTLIEKMSMTISEQ 180
           SHTNLTPNHLIR MIS+WC SRGIELQDRVHYLDDD ITEADR +   LIEKMS T+ EQ
Sbjct: 121 SHTNLTPNHLIRGMISRWCSSRGIELQDRVHYLDDDIITEADRGRFHRLIEKMSATLPEQ 180

Query: 181 KAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLS 240
           KAAAKELRMLTKRMPSFRALFGES DAIS LLSPLCGD SQSS+H DLQED+ITTLLNLS
Sbjct: 181 KAAAKELRMLTKRMPSFRALFGESHDAISLLLSPLCGDKSQSSVHADLQEDLITTLLNLS 240

Query: 241 IHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPL 300
           IHDNNKKLV+ETP+V+PLLMEALRSGTMETRSNAAAALFTLSALDSNK LIGK+GAL+PL
Sbjct: 241 IHDNNKKLVSETPRVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKVLIGKAGALKPL 300

Query: 301 IDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAM 360
           IDLLDEGHPL+IKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIM QMHVDELLAILAM
Sbjct: 301 IDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLAILAM 360

Query: 361 LSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKS 420
           LSNHPK+IEEMGEL AV  LLRIIRES CSRNKENCIVIIHAICLYDRT+WKD+REEEK 
Sbjct: 361 LSNHPKSIEEMGELRAVHSLLRIIRESTCSRNKENCIVIIHAICLYDRTQWKDMREEEKC 420

Query: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHT 456
           YRTISELAQNGTSRAKRKASGIL+RLNR VN THT
Sbjct: 421 YRTISELAQNGTSRAKRKASGILDRLNRVVNTTHT 455

[Close]

BLAST of MS017563.1 vs. ExPASy TrEMBL
Match: A0A6J1ESZ5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111437439 PE=4 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.6e-226
Identity = 404/455 (88.79%), Postives = 428/455 (94.07%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDS+PTV+GKAT+LK+ELQ+LI+AIIDDDN+S D ID AKEILCALKELKLRKR
Sbjct: 1   MAKTGVFDSNPTVVGKATDLKRELQKLIVAIIDDDNFSIDAIDQAKEILCALKELKLRKR 60

Query: 61  SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVL 120
           SRSSNLYENLICPD+FRCPLS ELM+DPVVVSTGET+DRPFI KWLK+GNRTCPRTQQVL
Sbjct: 61  SRSSNLYENLICPDEFRCPLSTELMKDPVVVSTGETFDRPFIQKWLKSGNRTCPRTQQVL 120

Query: 121 SHTNLTPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQLLTLIEKMSMTISEQ 180
           SHTNLTPNHLIR MIS+WC SRGIELQDRVHYLDDD ITEADR +  TLIEKMS T+ EQ
Sbjct: 121 SHTNLTPNHLIRGMISRWCSSRGIELQDRVHYLDDDIITEADRGRFHTLIEKMSATLPEQ 180

Query: 181 KAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLS 240
           KAAAKELRMLTKRMPSFRALFGES DAIS LLSPLCGD SQSS+H DLQED+ITTLLNLS
Sbjct: 181 KAAAKELRMLTKRMPSFRALFGESHDAISLLLSPLCGDKSQSSVHADLQEDLITTLLNLS 240

Query: 241 IHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPL 300
           IHDNNKKLV+ETPKV+PLLMEALRSGTMETRSNAAAALFTLSALDSNK LIGK+GAL+PL
Sbjct: 241 IHDNNKKLVSETPKVVPLLMEALRSGTMETRSNAAAALFTLSALDSNKVLIGKAGALKPL 300

Query: 301 IDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAM 360
           IDLLDEGHPL+IKDAASAIFNLCIIHENKARAV+DGAVRVLLKKIM QMHVD LLAILAM
Sbjct: 301 IDLLDEGHPLSIKDAASAIFNLCIIHENKARAVKDGAVRVLLKKIMNQMHVDALLAILAM 360

Query: 361 LSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKS 420
           LSNHPKAIEEMGEL AV  LLRIIRES CSRNKENCIVIIHAICLYDRT+WKD+REEEK 
Sbjct: 361 LSNHPKAIEEMGELRAVHSLLRIIRESTCSRNKENCIVIIHAICLYDRTQWKDMREEEKC 420

Query: 421 YRTISELAQNGTSRAKRKASGILERLNRAVNMTHT 456
           YRTISELAQNGTSRAKRKASGILERLNR VN THT
Sbjct: 421 YRTISELAQNGTSRAKRKASGILERLNRVVNTTHT 455

[Close]

BLAST of MS017563.1 vs. TAIR 10
Match: AT3G07360.1 (plant U-box 9 )

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-157
Identity = 288/464 (62.07%), Postives = 370/464 (79.74%), Query Frame = 0


Query: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60
           MAKTGVFDSDPT + KA ELK+E+++L++ I D+D+    TID  ++ L AL+E  +RK 
Sbjct: 1   MAKTGVFDSDPTAIAKAKELKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKM 60

Query: 61  SRSSNL--YENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQ 120
           ++SS+L   E + CP++FRCPLS ELMRDPVV+++G+TYD+ FI KWL +GNRTCP+TQQ
Sbjct: 61  AKSSSLEMLETVSCPEEFRCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQ 120

Query: 121 VLSHTNLTPNHLIREMISQWCKSRGIELQDRVH---YLDDDTITEADRDQLLTLIEKMSM 180
           VL HT LTPN LIREMIS+WCK  G+E + + H     +D+T+T +DR+   +L+ K+S 
Sbjct: 121 VLPHTALTPNLLIREMISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSS 180

Query: 181 T-ISEQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLC-GDNSQSSIHTDLQEDVI 240
           + + +QK+AAKELR+LT++   FRALFGES D I+ L++PL  G N        LQEDV+
Sbjct: 181 SNLQDQKSAAKELRLLTRKGTEFRALFGESPDEITRLVNPLLHGSNPDEK----LQEDVV 240

Query: 241 TTLLNLSIH-DNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIG 300
           TTLLN+SIH D+NKKLV E P VIPLL++ALR GT+ TRSNAAAA+FTLSALDSNK LIG
Sbjct: 241 TTLLNISIHDDSNKKLVCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIG 300

Query: 301 KSGALEPLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVD 360
           KSG L+PLIDLL+EG+PLAIKD A+AIF LCI HEN++RAVRDGAVRVL KKI   ++VD
Sbjct: 301 KSGILKPLIDLLEEGNPLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVD 360

Query: 361 ELLAILAMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWK 420
           ELLAILAML  H KA+EE+GELG V +LL+I RES C RNKEN IVI+H IC  DRTKWK
Sbjct: 361 ELLAILAMLVTHWKAVEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWK 420

Query: 421 DIREEEKSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 457
           +I+EEE ++ TI++L++ GTSRA+RKA+GIL+RL +A+N+THTA
Sbjct: 421 EIKEEENAHGTITKLSREGTSRAQRKANGILDRLRKAMNLTHTA 460

[Close]

BLAST of MS017563.1 vs. TAIR 10
Match: AT3G07360.2 (plant U-box 9 )

HSP 1 Score: 383.6 bits (984), Expect = 2.1e-106
Identity = 207/329 (62.92%), Postives = 263/329 (79.94%), Query Frame = 0


Query: 134 MISQWCKSRGIELQDRVH---YLDDDTITEADRDQLLTLIEKMSMT-ISEQKAAAKELRM 193
           MIS+WCK  G+E + + H     +D+T+T +DR+   +L+ K+S + + +QK+AAKELR+
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 194 LTKRMPSFRALFGESLDAISSLLSPLC-GDNSQSSIHTDLQEDVITTLLNLSIH-DNNKK 253
           LT++   FRALFGES D I+ L++PL  G N        LQEDV+TTLLN+SIH D+NKK
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPLLHGSNPDEK----LQEDVVTTLLNISIHDDSNKK 120

Query: 254 LVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPLIDLLDEG 313
           LV E P VIPLL++ALR GT+ TRSNAAAA+FTLSALDSNK LIGKSG L+PLIDLL+EG
Sbjct: 121 LVCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEG 180

Query: 314 HPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAMLSNHPKA 373
           +PLAIKD A+AIF LCI HEN++RAVRDGAVRVL KKI   ++VDELLAILAML  H KA
Sbjct: 181 NPLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKA 240

Query: 374 IEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKSYRTISEL 433
           +EE+GELG V +LL+I RES C RNKEN IVI+H IC  DRTKWK+I+EEE ++ TI++L
Sbjct: 241 VEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKL 300

Query: 434 AQNGTSRAKRKASGILERLNRAVNMTHTA 457
           ++ GTSRA+RKA+GIL+RL +A+N+THTA
Sbjct: 301 SREGTSRAQRKANGILDRLRKAMNLTHTA 325

[Close]

BLAST of MS017563.1 vs. TAIR 10
Match: AT3G07360.3 (plant U-box 9 )

HSP 1 Score: 383.6 bits (984), Expect = 2.1e-106
Identity = 207/329 (62.92%), Postives = 263/329 (79.94%), Query Frame = 0


Query: 134 MISQWCKSRGIELQDRVH---YLDDDTITEADRDQLLTLIEKMSMT-ISEQKAAAKELRM 193
           MIS+WCK  G+E + + H     +D+T+T +DR+   +L+ K+S + + +QK+AAKELR+
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 194 LTKRMPSFRALFGESLDAISSLLSPLC-GDNSQSSIHTDLQEDVITTLLNLSIH-DNNKK 253
           LT++   FRALFGES D I+ L++PL  G N        LQEDV+TTLLN+SIH D+NKK
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPLLHGSNPDEK----LQEDVVTTLLNISIHDDSNKK 120

Query: 254 LVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPLIDLLDEG 313
           LV E P VIPLL++ALR GT+ TRSNAAAA+FTLSALDSNK LIGKSG L+PLIDLL+EG
Sbjct: 121 LVCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEG 180

Query: 314 HPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAMLSNHPKA 373
           +PLAIKD A+AIF LCI HEN++RAVRDGAVRVL KKI   ++VDELLAILAML  H KA
Sbjct: 181 NPLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKA 240

Query: 374 IEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIREEEKSYRTISEL 433
           +EE+GELG V +LL+I RES C RNKEN IVI+H IC  DRTKWK+I+EEE ++ TI++L
Sbjct: 241 VEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKL 300

Query: 434 AQNGTSRAKRKASGILERLNRAVNMTHTA 457
           ++ GTSRA+RKA+GIL+RL +A+N+THTA
Sbjct: 301 SREGTSRAQRKANGILDRLRKAMNLTHTA 325

[Close]

BLAST of MS017563.1 vs. TAIR 10
Match: AT5G18320.1 (ARM repeat superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 5.9e-80
Identity = 180/450 (40.00%), Postives = 276/450 (61.33%), Query Frame = 0


Query: 17  ATELKKELQRLILAIIDD----DNYSTD-------TIDHAKEILCALKELKLRKRSRSSN 76
           A  L++ELQ+++  I++D    D   T+        ID A  IL  L++++ +      +
Sbjct: 9   ADTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLRKVESKIPESDIS 68

Query: 77  LYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQVLSHTNL 136
             E    P +F C LS  +M +PV++++G+TY++ +I +WLK   RTCP+T+QVLSH   
Sbjct: 69  PVE---VPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWLK-HERTCPKTKQVLSHRLW 128

Query: 137 TPNHLIREMISQWCKSRGIELQDRVHYLDDDTITEADRDQLLTLIEKM--SMTISEQKAA 196
            PNHLI ++I+QWC     + Q       D+ + E     +  L++++  S ++++Q  A
Sbjct: 129 IPNHLISDLITQWCLVNKYDHQKP----SDELVAELFTSDIEALLQRVSSSSSVADQIEA 188

Query: 197 AKELRMLTKRMPSFRALFGESL-DAISSLLSPLCGDNSQSSIHTDLQEDVITTLLNLSIH 256
           AKELR  TK+ P+ R  F   + D+I+ LLSPL   +       +LQE+++T L NLSI 
Sbjct: 189 AKELRHQTKKFPNVRVFFVAGIHDSITRLLSPLSTLDEAVDSSLELQENIVTALFNLSIL 248

Query: 257 DNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGALEPLID 316
           ++NK ++AE   VIPLL ++L+ GT ETR NAAA L +LSA+DSNK +IG S A++ LID
Sbjct: 249 ESNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVKALID 308

Query: 317 LLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLAILAMLS 376
           L++EG  LA K+A S +FNLCI+ ENK + V  G +    KKI    +VDELL++LA++S
Sbjct: 309 LIEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKIKAGSNVDELLSLLALIS 368

Query: 377 NHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAIC--LYDRTKWKDIREEEKS 436
            H +A+EEM +LG +  L  I+R+       EN +VI+  +     DR++ K + EEE  
Sbjct: 369 THNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGEEENQ 428

Query: 437 YRTISELAQNGTSRAKRKASGILERLNRAV 451
           + T ++LA+ G+ RA RKA GIL+ + R V
Sbjct: 429 HGTFTKLAKQGSVRAARKAQGILQWIKRFV 450

[Close]

BLAST of MS017563.1 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 275.8 bits (704), Expect = 6.3e-74
Identity = 181/475 (38.11%), Postives = 268/475 (56.42%), Query Frame = 0


Query: 6   VFDSDPTVMGKAT---------ELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELK 65
           V D DP ++ + +         ELKKE   +    +  D    D  +    +L  L +  
Sbjct: 165 VMDPDPIILKRLSQELQLTTIDELKKESHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFV 224

Query: 66  LRKR--------SRSSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKA 125
             +         SR  + + + + P+ FRCP+S ELM+DPV+VSTG+TY+R  I KWL A
Sbjct: 225 TMESSDPDPSTGSRIVSRHRSPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDA 284

Query: 126 GNRTCPRTQQVLSHTNLTPNHLIREMISQWCKSRGIELQD-----RVHYLDDDTITEADR 185
           G++TCP++Q+ L H  LTPN++++ +I+ WC+S GIEL       R   +   + ++ DR
Sbjct: 285 GHKTCPKSQETLLHAGLTPNYVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSSSDCDR 344

Query: 186 DQLLTLIEKMSM-TISEQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQS 245
             +L+L+EK++  T  +Q+AAA ELR+L KR    R    E+  AI  L+  L      S
Sbjct: 345 TFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEA-GAIPLLVELL------S 404

Query: 246 SIHTDLQEDVITTLLNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLS 305
           S     QE  +T LLNLSI++ NK  + +   +   ++E L++G+ME R NAAA LF+LS
Sbjct: 405 SPDPRTQEHSVTALLNLSINEGNKGAIVDAGAITD-IVEVLKNGSMEARENAAATLFSLS 464

Query: 306 ALDSNKALIGKSGALEPLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVL- 365
            +D NK  IG +GA++ LI LL+EG     KDAA+AIFNLCI   NK+RAV+ G V  L 
Sbjct: 465 VIDENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLT 524

Query: 366 -LKKIMKQMHVDELLAILAMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVII 425
            L K      VDE LAILA+LS + +    + E  ++P L+ IIR +   RN+EN   I+
Sbjct: 525 RLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEIIR-TGSPRNRENAAAIL 584

Query: 426 HAICLYDRTKWKDIREEEKSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHT 456
             +C+ +  +    RE       + EL +NGT RAKRKA+ +LE + +   +  T
Sbjct: 585 WYLCIGNIERLNVAREVGADV-ALKELTENGTDRAKRKAASLLELIQQTEGVAVT 629

The following BLAST results are available for this feature:

BLAST of MS017563.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159455.1	4.9e-254	99.78	U-box domain-containing protein 9 [Momordica charantia]	[more]
XP_022967253.1	6.9e-232	91.48	U-box domain-containing protein 9-like [Cucurbita maxima]	[more]
XP_038888241.1	6.9e-232	91.96	U-box domain-containing protein 9 [Benincasa hispida]	[more]
XP_022963574.1	6.9e-232	91.48	U-box domain-containing protein 9-like [Cucurbita moschata]	[more]
XP_023554208.1	1.5e-231	91.48	U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS017563.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRT0	1.7e-156	62.07	U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 S...	[more]
Q3E9F7	8.3e-79	40.00	Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=P...	[more]
Q5VRH9	1.0e-73	39.78	U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P...	[more]
Q8VZ40	8.9e-73	38.11	U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1...	[more]
Q8GUG9	5.9e-69	40.05	U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2...	[more]

BLAST of MS017563.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DYS9	2.4e-254	99.78	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102586...	[more]
A0A6J1HUJ6	3.3e-232	91.48	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466836 P...	[more]
A0A6J1HKK5	3.3e-232	91.48	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111463870...	[more]
A0A6J1K749	9.4e-227	89.01	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491272 P...	[more]
A0A6J1ESZ5	3.6e-226	88.79	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111437439...	[more]

BLAST of MS017563.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07360.1	1.2e-157	62.07	plant U-box 9	[more]
AT3G07360.2	2.1e-106	62.92	plant U-box 9	[more]
AT3G07360.3	2.1e-106	62.92	plant U-box 9	[more]
AT5G18320.1	5.9e-80	40.00	ARM repeat superfamily protein	[more]
AT3G54850.1	6.3e-74	38.11	plant U-box 14	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000225	Armadillo	SMART	SM00185	arm_5	coord: 242..283 e-value: 12.0 score: 12.7 coord: 364..405 e-value: 100.0 score: 5.4 coord: 284..324 e-value: 0.044 score: 22.9
IPR000225	Armadillo	PFAM	PF00514	Arm	coord: 287..323 e-value: 1.1E-6 score: 28.5
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 75..138 e-value: 6.0E-29 score: 112.2
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 73..142 e-value: 3.1E-21 score: 75.3
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 71..145 score: 38.454025
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 146..451 e-value: 6.5E-33 score: 115.9
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 71..145 e-value: 1.0E-27 score: 97.7
None	No IPR available	PANTHER	PTHR23315:SF253	U-BOX DOMAIN-CONTAINING PROTEIN 9	coord: 5..454
None	No IPR available	PANTHER	PTHR23315	U BOX DOMAIN-CONTAINING	coord: 5..454
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 76..118 e-value: 2.02225E-23 score: 90.3706
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 72..148
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 178..446

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS017563	MS017563	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS017563.1-cds	MS017563.1-cds-scaffold373:612801..613085	CDS
MS017563.1-cds	MS017563.1-cds-scaffold373:613817..614899	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS017563.1	MS017563.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity