MS010379 (gene) Bitter gourd (TR) v1

Overview


Name	MS010379
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	enoyl-CoA delta isomerase 2, peroxisomal-like
Location	scaffold878: 521039 .. 521755 (+)
RNA-Seq Expression	MS010379
Synteny	MS010379

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCACAGTTGAGAGGCGAGGCAATGTGTTCATCCTAACACTGACCGCCAACGACGATCACCGCCTCAACCCACCGTTGATCTACTCCATCCTCACCGCTCTTTCAGAGGTCAAAGCCAACGCCACCACCGGCTCCGTTCTCATCACAACCAACAATGGCGACTACTTCTCAAACGGCTTCGACCTCCCCTGGGCACTCGCCGCCGGCACCCTTGACGGCGCCCGTGACCGCCTCAACCACATAATTGACATCTTCAGGCCCGTCGTGGCCCAACTCCTTTCCCTTCGCATGCCTACCATTGCTGCCATTTCCGGCGACGCCGCTGCCGCCGGTCTCGCGTTGGCGGTGAGCCACGACTACTTGATCATGAGGAGCGATCTGGGCTCGCTGTTCATGCGAGAGATTGATCTGGGCCTCACTCTGCCGGACTACTTCGCGGCTCTGTTTAAGTCCAAGATGGGCTCCACTTCGGTTCAGCGCGACTTGATCCTGAGGGGCATGAAACTGAGCGGTGAAGCGGCGGTGAGAAGGGGCATTGTGGAGTCGGCGCACGACGGCGCCGGCGGCCTCATGACGGCTGCGCTCCAGTTTGGGGAGCAGCTGGCGGCGAAAAAATGGGATGGCGAAGCATATGCTGAAATCAGGAAGGGTTTGTACCCTGAGATCACTGGGGTGCTTGGATTACCCACCAAGGTCATGACCATTTCCAAACTC

mRNA sequence

ATGTGCACAGTTGAGAGGCGAGGCAATGTGTTCATCCTAACACTGACCGCCAACGACGATCACCGCCTCAACCCACCGTTGATCTACTCCATCCTCACCGCTCTTTCAGAGGTCAAAGCCAACGCCACCACCGGCTCCGTTCTCATCACAACCAACAATGGCGACTACTTCTCAAACGGCTTCGACCTCCCCTGGGCACTCGCCGCCGGCACCCTTGACGGCGCCCGTGACCGCCTCAACCACATAATTGACATCTTCAGGCCCGTCGTGGCCCAACTCCTTTCCCTTCGCATGCCTACCATTGCTGCCATTTCCGGCGACGCCGCTGCCGCCGGTCTCGCGTTGGCGGTGAGCCACGACTACTTGATCATGAGGAGCGATCTGGGCTCGCTGTTCATGCGAGAGATTGATCTGGGCCTCACTCTGCCGGACTACTTCGCGGCTCTGTTTAAGTCCAAGATGGGCTCCACTTCGGTTCAGCGCGACTTGATCCTGAGGGGCATGAAACTGAGCGGTGAAGCGGCGGTGAGAAGGGGCATTGTGGAGTCGGCGCACGACGGCGCCGGCGGCCTCATGACGGCTGCGCTCCAGTTTGGGGAGCAGCTGGCGGCGAAAAAATGGGATGGCGAAGCATATGCTGAAATCAGGAAGGGTTTGTACCCTGAGATCACTGGGGTGCTTGGATTACCCACCAAGGTCATGACCATTTCCAAACTC

Coding sequence (CDS)

ATGTGCACAGTTGAGAGGCGAGGCAATGTGTTCATCCTAACACTGACCGCCAACGACGATCACCGCCTCAACCCACCGTTGATCTACTCCATCCTCACCGCTCTTTCAGAGGTCAAAGCCAACGCCACCACCGGCTCCGTTCTCATCACAACCAACAATGGCGACTACTTCTCAAACGGCTTCGACCTCCCCTGGGCACTCGCCGCCGGCACCCTTGACGGCGCCCGTGACCGCCTCAACCACATAATTGACATCTTCAGGCCCGTCGTGGCCCAACTCCTTTCCCTTCGCATGCCTACCATTGCTGCCATTTCCGGCGACGCCGCTGCCGCCGGTCTCGCGTTGGCGGTGAGCCACGACTACTTGATCATGAGGAGCGATCTGGGCTCGCTGTTCATGCGAGAGATTGATCTGGGCCTCACTCTGCCGGACTACTTCGCGGCTCTGTTTAAGTCCAAGATGGGCTCCACTTCGGTTCAGCGCGACTTGATCCTGAGGGGCATGAAACTGAGCGGTGAAGCGGCGGTGAGAAGGGGCATTGTGGAGTCGGCGCACGACGGCGCCGGCGGCCTCATGACGGCTGCGCTCCAGTTTGGGGAGCAGCTGGCGGCGAAAAAATGGGATGGCGAAGCATATGCTGAAATCAGGAAGGGTTTGTACCCTGAGATCACTGGGGTGCTTGGATTACCCACCAAGGTCATGACCATTTCCAAACTC

Protein sequence

MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNGFDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHDYLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGIVESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL

Homology

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BLAST of MS010379 vs. NCBI nr
Match: XP_022143599.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Momordica charantia] >XP_022143606.1 enoyl-CoA delta isomerase 2, peroxisomal-like [Momordica charantia])

HSP 1 Score: 461.1 bits (1185), Expect = 5.9e-126
Identity = 238/239 (99.58%), Postives = 238/239 (99.58%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGD FSNG
Sbjct: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD
Sbjct: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI
Sbjct: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL
Sbjct: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 239

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BLAST of MS010379 vs. NCBI nr
Match: KAG6582371.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 352.8 bits (904), Expect = 2.3e-93
Identity = 179/239 (74.90%), Postives = 205/239 (85.77%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCTVE+RGN FILTLT +DDHRL P LI SIL ALS+VKA AT+GSVLITT++G +FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRLNHII IF+P+VAQLLSL MPTIAAI+G AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           VES H G  G+  AA+Q GEQLAA+KW+GE YAEIRK LYPE++ VLGL  K ++ISKL
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239

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BLAST of MS010379 vs. NCBI nr
Match: XP_023526416.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 352.4 bits (903), Expect = 3.0e-93
Identity = 178/239 (74.48%), Postives = 204/239 (85.36%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCTVE+RGN FILTLT +DDHRL P LI S+L ALS+VKA AT GSVLITT++G +FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRL+HII IF+P+VAQLLSL MPTIAAISG AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLSHIIQIFKPLVAQLLSLPMPTIAAISGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLAMRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           VES H G  G+  AA+Q GEQLAA+KWDGE YAEIRK +YPE++ VLGL  K ++ISKL
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWDGEVYAEIRKSVYPEVSEVLGLRGKAISISKL 239

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BLAST of MS010379 vs. NCBI nr
Match: XP_022979202.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 350.5 bits (898), Expect = 1.1e-92
Identity = 178/239 (74.48%), Postives = 203/239 (84.94%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCT+E+RGN FILTLT +DDHRL P LI SIL ALS+VKA AT GSVLITT++G +FSNG
Sbjct: 1   MCTLEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRLNHII IF+P+VAQLLSL MPTIAAI+G AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALF SKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFMSKMGSSLAMRDLILRGMKVRGEAAVRIGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           VES H G  G+  AA+Q GEQLAA+KWDGE YAEIRK LYPE++ VLGL  K ++ISKL
Sbjct: 181 VESTHHGEDGVANAAVQLGEQLAARKWDGEVYAEIRKSLYPEVSEVLGLRGKAISISKL 239

[Close]

BLAST of MS010379 vs. NCBI nr
Match: XP_022924234.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 349.0 bits (894), Expect = 3.3e-92
Identity = 177/239 (74.06%), Postives = 203/239 (84.94%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCTVE+RGN FILTLT +DDHRL P LI S+L ALS+VKA AT GSVLITT++G +FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRLNHII IF+P+VAQLLSL MPTIAAI+G AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           VES H G  G+  AA+Q GEQLAA+KW+GE YAEIRK LYPE++ VLGL  K + IS+L
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPIFISRL 239

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BLAST of MS010379 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 3.0e-72
Identity = 132/240 (55.00%), Postives = 185/240 (77.08%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCT+E+RG++F+LTLT + +HR +P  I +IL+ L + K+ +T GS+LITT NG +FSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDL WA  AG+  GA +RL+ +++ F+PVVA LL L MPTIAA++G AAAAGL LA+SHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           Y+ MR D G L+M E+D+GL++PDYF+AL ++K+G+++ +R+L+L G K+ GE AV  GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           V+S A+D   G++ A+++ GE+LAAKKW GE YA IRK LYPE+ G+LGL T+V    KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of MS010379 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 2.7e-65
Identity = 126/230 (54.78%), Postives = 170/230 (73.91%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCT+E+RG++F+LTLT  D+HR +P  I S+L+ L + K+ +T GSVLITT +G +FSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDL WA +AG   GA  R++ ++  F+PV+A LL L MPTIAA++G AAA+GL  A+SHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           Y+ MR D G L+M E+D+GL +PDYF+AL  +K+GS   +R+L+L G KL GE AV  GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGL 230
           V+S AHD A G++ A +  GE LAAKKW+GE YA IRK LYPE+  ++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

[Close]

BLAST of MS010379 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 2.2e-43
Identity = 91/224 (40.62%), Postives = 151/224 (67.41%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANAT-TGSVLITTNNGDYFSN 60
           MC++E+R  +FIL LT + +HRLNP L+ S+ + +++++++ + + SVLITT++G +FSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSH 120
           G+DL  A +  +L    D         R +VA L+SL MPTIAA++G A+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRG 180
           DY++MR D G L+M E+D+ L +P +F A+ + K+GS + +RD++L   K++ +  V+ G
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEI 224
           IV+SA+  A   + AA++ GE++  +  DG  Y ++R+ L  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

[Close]

BLAST of MS010379 vs. ExPASy Swiss-Prot
Match: P64019 (Probable enoyl-CoA hydratase echA14 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=echA14 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.9e-06
Identity = 64/197 (32.49%), Postives = 88/197 (44.67%), Query Frame = 0


Query: 12  ILTLTANDDHRLN---PPLIYSILTALSEVKANATTGSVLITTNNGDYFSNGFDLPWALA 71
           +  +T ND  R N     +   +  A+   + +    +V++ T  G  F  G DL  AL 
Sbjct: 15  VALITVNDPDRRNAVTDEMSAQLRAAIQRAEGDPDVHAVVV-TGAGKAFCAGADLS-ALG 74

Query: 72  AGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHDYLIMRSDL 131
           AG  D A  RL  + D F  V     S  +PTIAA++G A  AGL LA++ D  I  +  
Sbjct: 75  AGVGDPAEPRLLRLYDGFMAV----SSCNLPTIAAVNGAAVGAGLNLALAADVRI--AGP 134

Query: 132 GSLF-MREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGIVESAHDG 191
            +LF  R   LGL  P   A     +     V R  +L GM    E+AVR G+     D 
Sbjct: 135 AALFDARFQKLGLH-PGGGATWMLQRAVGPQVARAALLFGMCFDAESAVRHGLALMVADD 194

Query: 192 AGGLMTAALQFGEQLAA 205
               +TAAL+     AA
Sbjct: 195 P---VTAALELAAGPAA 199

[Close]

BLAST of MS010379 vs. ExPASy Swiss-Prot
Match: P9WNN4 (Probable enoyl-CoA hydratase echA14 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=echA14 PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.9e-06
Identity = 64/197 (32.49%), Postives = 88/197 (44.67%), Query Frame = 0


Query: 12  ILTLTANDDHRLN---PPLIYSILTALSEVKANATTGSVLITTNNGDYFSNGFDLPWALA 71
           +  +T ND  R N     +   +  A+   + +    +V++ T  G  F  G DL  AL 
Sbjct: 15  VALITVNDPDRRNAVTDEMSAQLRAAIQRAEGDPDVHAVVV-TGAGKAFCAGADLS-ALG 74

Query: 72  AGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHDYLIMRSDL 131
           AG  D A  RL  + D F  V     S  +PTIAA++G A  AGL LA++ D  I  +  
Sbjct: 75  AGVGDPAEPRLLRLYDGFMAV----SSCNLPTIAAVNGAAVGAGLNLALAADVRI--AGP 134

Query: 132 GSLF-MREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGIVESAHDG 191
            +LF  R   LGL  P   A     +     V R  +L GM    E+AVR G+     D 
Sbjct: 135 AALFDARFQKLGLH-PGGGATWMLQRAVGPQVARAALLFGMCFDAESAVRHGLALMVADD 194

Query: 192 AGGLMTAALQFGEQLAA 205
               +TAAL+     AA
Sbjct: 195 P---VTAALELAAGPAA 199

[Close]

BLAST of MS010379 vs. ExPASy TrEMBL
Match: A0A6J1CQS5 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=LOC111013455 PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 2.9e-126
Identity = 238/239 (99.58%), Postives = 238/239 (99.58%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGD FSNG
Sbjct: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDNFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD
Sbjct: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI
Sbjct: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL
Sbjct: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 239

[Close]

BLAST of MS010379 vs. ExPASy TrEMBL
Match: A0A6J1IN54 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111479008 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 5.5e-93
Identity = 178/239 (74.48%), Postives = 203/239 (84.94%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCT+E+RGN FILTLT +DDHRL P LI SIL ALS+VKA AT GSVLITT++G +FSNG
Sbjct: 1   MCTLEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRLNHII IF+P+VAQLLSL MPTIAAI+G AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALF SKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFMSKMGSSLAMRDLILRGMKVRGEAAVRIGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           VES H G  G+  AA+Q GEQLAA+KWDGE YAEIRK LYPE++ VLGL  K ++ISKL
Sbjct: 181 VESTHHGEDGVANAAVQLGEQLAARKWDGEVYAEIRKSLYPEVSEVLGLRGKAISISKL 239

[Close]

BLAST of MS010379 vs. ExPASy TrEMBL
Match: A0A6J1E8J9 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431761 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.6e-92
Identity = 177/239 (74.06%), Postives = 203/239 (84.94%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCTVE+RGN FILTLT +DDHRL P LI S+L ALS+VKA AT GSVLITT++G +FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++ GARDRLNHII IF+P+VAQLLSL MPTIAAI+G AAAAG ALA+SHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSKMGS+   RDLILRGMK+ GEAAVR G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           VES H G  G+  AA+Q GEQLAA+KW+GE YAEIRK LYPE++ VLGL  K + IS+L
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPIFISRL 239

[Close]

BLAST of MS010379 vs. ExPASy TrEMBL
Match: A0A0A0L9X4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 6.3e-89
Identity = 169/239 (70.71%), Postives = 202/239 (84.52%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCTVE+RGN FILTLT +DDHRL+P LI SIL ALS+VKA A+  SVLITT++G +FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWAL+AG++  AR RLNH+I+IF+ VVA+L+SL MPTIAAI+G AAAAG  LA+SHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSK+GS+   RDLILRGMK+ GEAAVR GI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           V+SAHDG   ++ AA++ GEQLA + WDGE YAEIRK LYPE++ VLGLP K ++ISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

[Close]

BLAST of MS010379 vs. ExPASy TrEMBL
Match: A0A1S3AWB3 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103483618 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.2e-87
Identity = 169/239 (70.71%), Postives = 199/239 (83.26%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCTVE+RGN FILTLT +DDHRL+P LI SIL ALS+VKA A+  SVLITT++G +FSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDLPWALAAG++  AR RLNHII+IF+ VVA+L+SL MPTIAAI+G AAAAG  LA+SHD
Sbjct: 61  FDLPWALAAGSVSAARHRLNHIIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           YLIMRSD G L+MRE+DLGLTLPDYF ALFKSK+GS+   RDLILRGMK+ GEAAVR GI
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           V+SAH     ++ AA++ GEQL  + WDGE YAEIRK LYPEI+ VLGLP K ++ISKL
Sbjct: 181 VDSAHHSEVSVVNAAVRLGEQLVGRNWDGEVYAEIRKSLYPEISEVLGLPGKAISISKL 239

[Close]

BLAST of MS010379 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 273.1 bits (697), Expect = 2.1e-73
Identity = 132/240 (55.00%), Postives = 185/240 (77.08%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCT+E+RG++F+LTLT + +HR +P  I +IL+ L + K+ +T GS+LITT NG +FSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDL WA  AG+  GA +RL+ +++ F+PVVA LL L MPTIAA++G AAAAGL LA+SHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           Y+ MR D G L+M E+D+GL++PDYF+AL ++K+G+++ +R+L+L G K+ GE AV  GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGLPTKVMTISKL 240
           V+S A+D   G++ A+++ GE+LAAKKW GE YA IRK LYPE+ G+LGL T+V    KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of MS010379 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 250.0 bits (637), Expect = 1.9e-66
Identity = 126/230 (54.78%), Postives = 170/230 (73.91%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANATTGSVLITTNNGDYFSNG 60
           MCT+E+RG++F+LTLT  D+HR +P  I S+L+ L + K+ +T GSVLITT +G +FSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSHD 120
           FDL WA +AG   GA  R++ ++  F+PV+A LL L MPTIAA++G AAA+GL  A+SHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRGI 180
           Y+ MR D G L+M E+D+GL +PDYF+AL  +K+GS   +R+L+L G KL GE AV  GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEITGVLGL 230
           V+S AHD A G++ A +  GE LAAKKW+GE YA IRK LYPE+  ++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

[Close]

BLAST of MS010379 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 177.2 bits (448), Expect = 1.6e-44
Identity = 91/224 (40.62%), Postives = 151/224 (67.41%), Query Frame = 0


Query: 1   MCTVERRGNVFILTLTANDDHRLNPPLIYSILTALSEVKANAT-TGSVLITTNNGDYFSN 60
           MC++E+R  +FIL LT + +HRLNP L+ S+ + +++++++ + + SVLITT++G +FSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWALAAGTLDGARDRLNHIIDIFRPVVAQLLSLRMPTIAAISGDAAAAGLALAVSH 120
           G+DL  A +  +L    D         R +VA L+SL MPTIAA++G A+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDLGSLFMREIDLGLTLPDYFAALFKSKMGSTSVQRDLILRGMKLSGEAAVRRG 180
           DY++MR D G L+M E+D+ L +P +F A+ + K+GS + +RD++L   K++ +  V+ G
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGAGGLMTAALQFGEQLAAKKWDGEAYAEIRKGLYPEI 224
           IV+SA+  A   + AA++ GE++  +  DG  Y ++R+ L  E+
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

The following BLAST results are available for this feature:

BLAST of MS010379 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143599.1	5.9e-126	99.58	enoyl-CoA delta isomerase 2, peroxisomal-like [Momordica charantia] >XP_02214360...	[more]
KAG6582371.1	2.3e-93	74.90	Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023526416.1	3.0e-93	74.48	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo]	[more]
XP_022979202.1	1.1e-92	74.48	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima]	[more]
XP_022924234.1	3.3e-92	74.06	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata]	[more]

BLAST of MS010379 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23299	3.0e-72	55.00	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2...	[more]
O23300	2.7e-65	54.78	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1	[more]
O04469	2.2e-43	40.63	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1...	[more]
P64019	2.9e-06	32.49	Probable enoyl-CoA hydratase echA14 OS=Mycobacterium bovis (strain ATCC BAA-935 ...	[more]
P9WNN4	2.9e-06	32.49	Probable enoyl-CoA hydratase echA14 OS=Mycobacterium tuberculosis (strain CDC 15...	[more]

BLAST of MS010379 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQS5	2.9e-126	99.58	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1IN54	5.5e-93	74.48	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1E8J9	1.6e-92	74.06	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A0A0L9X4	6.3e-89	70.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1	[more]
A0A1S3AWB3	1.2e-87	70.71	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]

BLAST of MS010379 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14430.1	2.1e-73	55.00	indole-3-butyric acid response 10	[more]
AT4G14440.1	1.9e-66	54.78	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	1.6e-44	40.63	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 21..206 e-value: 1.5E-15 score: 57.2
None	No IPR available	GENE3D	3.90.226.10		coord: 1..226 e-value: 8.0E-34 score: 118.9
None	No IPR available	PANTHER	PTHR11941:SF75	ENOYL-COA DELTA ISOMERASE 2, PEROXISOMAL	coord: 1..233
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 1..233
None	No IPR available	CDD	cd06558	crotonase-like	coord: 2..202 e-value: 1.57653E-16 score: 72.9784
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 3..207

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010379.1	MS010379.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity