MS006120 (gene) Bitter gourd (TR) v1

Overview


Name	MS006120
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Riboflavin kinase
Location	scaffold96: 190985 .. 194271 (+)
RNA-Seq Expression	MS006120
Synteny	MS006120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTGTATCTGGCGTCATTCTTGATCTGGATGGAACACTTCTAAATACAGGTCCTATTGTATTCTCAACAATGTGGATCAACTTTCGTATTGCTTACTGTTGTTACATAATACTTGTTTCTATCAGCAACTCTATTTTAAGGCATTGAAATTGTTTTTTTCTGCTTGTGAAAAATGTGTTATCTATTTGATAGCTTCCATACCTAGACTCCCTATTTCGACTTGGTATTGTATAAATATATTAACCAGCTAGCAATTGTGTTAACTTTTAGTGTCCTTGTCTTTCCTTTTCTCTCCACTGCCGAATTTCTCTCTCCCACACCAAACTATAGCAGATGGCATAGTGAATGAAGTTCTAAAATCTTTTTTGGGCAAGTATGGAAAGCAATGGGATGGAAGAGAAGCCCAAAGGGTTGTTGGGAAGACACCATTTGAGTCTGCAGCTGTCATCATTGAAGATTACGGGCTTTCTTGCACATCAGCCGAACTGATGTCACAGATTTCCCCTTTGTTTGCGGAGAGGTAAGTAACTATTTTGCTTTCCTCTTGGTTATAAGTTCTTCAACCGTTTAAGGTCAATAGTGAGATTTCATGGTTTTACATTATGTTGCAAATTGCTGTGGTGATACACAGTGGCAGTTCTAAATTGAAAACACTGCATTCATTCCAAACGCGACATGATTTTTCTGGGTTGCGACCTGTGGCAATGTCATATGCTTTAAACTCTCTTGTCTCGGCAAACTTACAAACAACTGGTGTTGTTCTAATTACCATCTGTAAATTTATGAGCATATTTATTAATCCGTAGTTTGTGGCACCATATATATTATGTTGTTTATGTTAAATTAACAGCTCGTTGATCTTTGAACACTGGTTTTTAGCTTTTCAAGTTACTTGGAACTGGATATTTCTTGTTAGTTTGGCTTTGGTTATGAAAATAGCATAAAGCATTTATCAGACTATTTATTTTTTCTTGGACCTCCCAATTTACTTTTGATATTTTTTCCTGAATTTTCTTGGCTCGGTGAATTGTTGTTTCATTTCATTGCCTGGATCATGGTGCTATTTGCTAAATACAAGACTTCAATCTCAGATCGTGTATTGTAATGGTTACTTTGTTATTATTAAGAAGTGTTTTGAGTAATGGAACTTCTTAACACATAGAATTGGTCAGACCACACCGTATGCATTTGAAGGCAGATGTTACTGGGTGGGTTGGAATTTATAATAATTCAGCATTTTATGTTCTTTGAACTACATAATATTAATTAGTTCATGAGGTTATTATTTCTTTATTTTTCTTTGGGCAGTTTCATTGAGGAAATGGAATTTTATTATTCTATTGGATTGTTGATTATGCAAATGTATTACATCTACAATATGATCCATGTCATGCCAGAATACTTTTAGTTTGGAAGAACTTTTCTTTAGGCGTCTTTTGGTGATTTCTACTTCAGGTGGTGCAACATTAAAGCACTTCCAGGAGCAAATCGGTTGATTAAACATTTCACTGGTCACAGAGTGCCAATTGCATTGGCTTCAAACTCAACTAGGGAAAACATAGAAACCAAAATTTCTTTTCATCCGGGTATGTTTGCTCAGAGAATGTAACATGTTTATTTGTTCCGATTGAAAGTGCTGATCATTGAGGCTTTTACACTCGTTTGTGATTGCTATTTATGTATTCAGCTTCTTGATTTTATGATTGCATAGGCTGGAAAGACTCATTTTCTGTGATCATTGGTAGCGATGAAGTTAGAACGGGCAAACCGTCTCCTGAAATGTAAGATGCAACTATTTTCTGTCTACTCTCTCTAATTTGATTAAAAGTTTCCTTTGCAAGTAGCCCATTAAGAACTGACAATGTATAGCCATTTCAGATGGCTGACTGCTCTTCAAAATTTCTTTAAGTATTTCCTTTTCAAGCAATCAATCAACAGTTCTAACAATGCTTTCATTGTTAAAGATTCCTTGAATCAGCAAAAAGGTTAAATCTTGAACCCTCAAGGTGCCTCGTAATTGAGGATTCCGTGTAAGCATCTGATCTATTCAAATTTTAAATATTGGAATTTCCTATGTGAAGATATTAACTCACAGTCTATAACTTCTCTCTCAACATGGTCCACCAGTCCAGGTGTTACAGCTGGTAAAGCTGCTGGGATGAAGGTGGTTGCTGTACCATCCCTTCAGAAAAAGTCTCATCTCTACTCTTCAGCAGATGAGGTGATCAATTCACTACTTGACTTTCAGCCTGAAAAGTGGGGCCTGCCTCCATTTGAAGATTGTATATACCACAAACCTTTTTAACCATCTCAATTCTCAACCTTTCCAGTTGAGTTTTTCTGTGCATCAACCTTAATTTTTGTCACAATTCTTGACTTTTGACTATAGGGGTAGAGAACACCTTACCAATTAATCCTTTGTACATTGGAGGTCCCGTTGTCAAGGGATATGGGCGTGGCTCAAAAGTACTTGGGATTCCTACAGGTGTGTGATAATTCAACAGACCTTTCCCATTTCAGTAGGTTGCTCATTTGACCTTAAAATCAGCAGTATGTAAGAATTGCCAAACTTATCCATCTGGTGTTCACTTACCAAACTTTCTGGAGTTATTTGATTCCTGGTCTCATCTCATAGTCCTTATTGAGCAAACTTAAGAGTAACATGCGAAAGAGTGCTCTTCCTGTACTGTTATTAACAATTTTCTGCAAGCAGATTTCTTTCCATTATAATACCTACAAACTTGTCTTGCTTTTCCCAGCGAATTTATCTGCAGAAGGATATTCAGATGTCCTTTCAGAACATCCATCGGGCGTGTATTTCGGTTGGGCCGGTTTATCAATGAGAGGCGTCTTTAAAATGGTCATGAGCATTGGTTGGAATCCATTTTTTGACAATAAAGAAAAGACTATTGTAAGTATAACAGTTCAGAATGTTAGGTTGCTGTATCACTTTCTGGTTAATATTCATATTCGATACAGGAACCATGGCTACTTCATGAATTTGATGACGATTTCTACAACGAGGACTTGAGACTTGTTGTCGTTGGATATATTCGGCCAGAGGTATTATTTGTAAAATAACTAGCATGTTCTAAATTGTTGATTGCCATGCTCACCACTTTCACTAAAATCTCGGCAATATTATCACTAAGCAGGCCAACTTTCCAACCCTTGAGAGTCTCGTGGCTAAGATTCACGAGGATGGGAGAATTGCAGAGAGAGCTCTTGACCTTCCATTGTACTCGAAGTATAGGAACGAGCAGTACCTGAAAGAGCATGATGCT

mRNA sequence

ATGATTGTATCTGGCGTCATTCTTGATCTGGATGGAACACTTCTAAATACAGCAGATGGCATAGTGAATGAAGTTCTAAAATCTTTTTTGGGCAAGTATGGAAAGCAATGGGATGGAAGAGAAGCCCAAAGGGTTGTTGGGAAGACACCATTTGAGTCTGCAGCTGTCATCATTGAAGATTACGGGCTTTCTTGCACATCAGCCGAACTGATGTCACAGATTTCCCCTTTGTTTGCGGAGAGGTGGTGCAACATTAAAGCACTTCCAGGAGCAAATCGGTTGATTAAACATTTCACTGGTCACAGAGTGCCAATTGCATTGGCTTCAAACTCAACTAGGGAAAACATAGAAACCAAAATTTCTTTTCATCCGGGCTGGAAAGACTCATTTTCTGTGATCATTGGTAGCGATGAAGTTAGAACGGGCAAACCGTCTCCTGAAATATTCCTTGAATCAGCAAAAAGGTTAAATCTTGAACCCTCAAGGTGCCTCGTAATTGAGGATTCCGTTCCAGGTGTTACAGCTGGTAAAGCTGCTGGGATGAAGGTGGTTGCTGTACCATCCCTTCAGAAAAAGTCTCATCTCTACTCTTCAGCAGATGAGGTGATCAATTCACTACTTGACTTTCAGCCTGAAAAGTGGGGCCTGCCTCCATTTGAAGATTGGGTAGAGAACACCTTACCAATTAATCCTTTGTACATTGGAGGTCCCGTTGTCAAGGGATATGGGCGTGGCTCAAAAGTACTTGGGATTCCTACAGCGAATTTATCTGCAGAAGGATATTCAGATGTCCTTTCAGAACATCCATCGGGCGTGTATTTCGGTTGGGCCGGTTTATCAATGAGAGGCGTCTTTAAAATGGTCATGAGCATTGGTTGGAATCCATTTTTTGACAATAAAGAAAAGACTATTGAACCATGGCTACTTCATGAATTTGATGACGATTTCTACAACGAGGACTTGAGACTTGTTGTCGTTGGATATATTCGGCCAGAGGCCAACTTTCCAACCCTTGAGAGTCTCGTGGCTAAGATTCACGAGGATGGGAGAATTGCAGAGAGAGCTCTTGACCTTCCATTGTACTCGAAGTATAGGAACGAGCAGTACCTGAAAGAGCATGATGCT

Coding sequence (CDS)

ATGATTGTATCTGGCGTCATTCTTGATCTGGATGGAACACTTCTAAATACAGCAGATGGCATAGTGAATGAAGTTCTAAAATCTTTTTTGGGCAAGTATGGAAAGCAATGGGATGGAAGAGAAGCCCAAAGGGTTGTTGGGAAGACACCATTTGAGTCTGCAGCTGTCATCATTGAAGATTACGGGCTTTCTTGCACATCAGCCGAACTGATGTCACAGATTTCCCCTTTGTTTGCGGAGAGGTGGTGCAACATTAAAGCACTTCCAGGAGCAAATCGGTTGATTAAACATTTCACTGGTCACAGAGTGCCAATTGCATTGGCTTCAAACTCAACTAGGGAAAACATAGAAACCAAAATTTCTTTTCATCCGGGCTGGAAAGACTCATTTTCTGTGATCATTGGTAGCGATGAAGTTAGAACGGGCAAACCGTCTCCTGAAATATTCCTTGAATCAGCAAAAAGGTTAAATCTTGAACCCTCAAGGTGCCTCGTAATTGAGGATTCCGTTCCAGGTGTTACAGCTGGTAAAGCTGCTGGGATGAAGGTGGTTGCTGTACCATCCCTTCAGAAAAAGTCTCATCTCTACTCTTCAGCAGATGAGGTGATCAATTCACTACTTGACTTTCAGCCTGAAAAGTGGGGCCTGCCTCCATTTGAAGATTGGGTAGAGAACACCTTACCAATTAATCCTTTGTACATTGGAGGTCCCGTTGTCAAGGGATATGGGCGTGGCTCAAAAGTACTTGGGATTCCTACAGCGAATTTATCTGCAGAAGGATATTCAGATGTCCTTTCAGAACATCCATCGGGCGTGTATTTCGGTTGGGCCGGTTTATCAATGAGAGGCGTCTTTAAAATGGTCATGAGCATTGGTTGGAATCCATTTTTTGACAATAAAGAAAAGACTATTGAACCATGGCTACTTCATGAATTTGATGACGATTTCTACAACGAGGACTTGAGACTTGTTGTCGTTGGATATATTCGGCCAGAGGCCAACTTTCCAACCCTTGAGAGTCTCGTGGCTAAGATTCACGAGGATGGGAGAATTGCAGAGAGAGCTCTTGACCTTCCATTGTACTCGAAGTATAGGAACGAGCAGTACCTGAAAGAGCATGATGCT

Protein sequence

MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIEDYGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKISFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAGMKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVKGYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNKEKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPLYSKYRNEQYLKEHDA

Homology

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BLAST of MS006120 vs. NCBI nr
Match: XP_022140231.1 (bifunctional riboflavin kinase/FMN phosphatase isoform X1 [Momordica charantia])

HSP 1 Score: 766.9 bits (1979), Expect = 7.9e-218
Identity = 375/375 (100.00%), Postives = 375/375 (100.00%), Query Frame = 0


Query: 1   MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 60
           MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED
Sbjct: 12  MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 71

Query: 61  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 120
           YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI
Sbjct: 72  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 131

Query: 121 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 180
           SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG
Sbjct: 132 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 191

Query: 181 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 240
           MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK
Sbjct: 192 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 251

Query: 241 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 300
           GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK
Sbjct: 252 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 311

Query: 301 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 360
           EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL
Sbjct: 312 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 371

Query: 361 YSKYRNEQYLKEHDA 376
           YSKYRNEQYLKEHDA
Sbjct: 372 YSKYRNEQYLKEHDA 386

[Close]

BLAST of MS006120 vs. NCBI nr
Match: XP_022140232.1 (bifunctional riboflavin kinase/FMN phosphatase isoform X2 [Momordica charantia])

HSP 1 Score: 760.4 bits (1962), Expect = 7.4e-216
Identity = 374/375 (99.73%), Postives = 374/375 (99.73%), Query Frame = 0


Query: 1   MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 60
           MIVSGVILDLDGTLLNT DGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED
Sbjct: 12  MIVSGVILDLDGTLLNT-DGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 71

Query: 61  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 120
           YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI
Sbjct: 72  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 131

Query: 121 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 180
           SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG
Sbjct: 132 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 191

Query: 181 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 240
           MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK
Sbjct: 192 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 251

Query: 241 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 300
           GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK
Sbjct: 252 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 311

Query: 301 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 360
           EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL
Sbjct: 312 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 371

Query: 361 YSKYRNEQYLKEHDA 376
           YSKYRNEQYLKEHDA
Sbjct: 372 YSKYRNEQYLKEHDA 385

[Close]

BLAST of MS006120 vs. NCBI nr
Match: XP_004146584.1 (bifunctional riboflavin kinase/FMN phosphatase [Cucumis sativus] >XP_011653006.1 bifunctional riboflavin kinase/FMN phosphatase [Cucumis sativus] >KGN64630.1 hypothetical protein Csa_014197 [Cucumis sativus])

HSP 1 Score: 704.1 bits (1816), Expect = 6.3e-199
Identity = 341/375 (90.93%), Postives = 358/375 (95.47%), Query Frame = 0


Query: 1   MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 60
           M+VSGVILDLDGTLL+T DGIVN+VLKSFLGKYGKQWDGREA RV GKTP+ESAAVI+ED
Sbjct: 13  MVVSGVILDLDGTLLHT-DGIVNDVLKSFLGKYGKQWDGREALRVTGKTPYESAAVIVED 72

Query: 61  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 120
           YGL C+SAELMSQISPLFAERWCNIKALPGANRLIKHF+ HRVPIALASNS+RENIE+KI
Sbjct: 73  YGLPCSSAELMSQISPLFAERWCNIKALPGANRLIKHFSNHRVPIALASNSSRENIESKI 132

Query: 121 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 180
           SFHPGWKDSFSVIIGS+EV   KPSPEIFLESAKRLNLEPS CLVIEDSVPGV AGKAAG
Sbjct: 133 SFHPGWKDSFSVIIGSNEVTAAKPSPEIFLESAKRLNLEPSSCLVIEDSVPGVAAGKAAG 192

Query: 181 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 240
           MKVVAVPSL KKSHLYSSADEVINSLLDFQP+KWGLPPFEDWVENTLPINPLYIGGPVVK
Sbjct: 193 MKVVAVPSLPKKSHLYSSADEVINSLLDFQPQKWGLPPFEDWVENTLPINPLYIGGPVVK 252

Query: 241 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 300
           GYGRGSKVLGIPTANLS EGYSDVLSEHPSGVYFGWAGLS RG+FKMVMSIGWNPFFDN 
Sbjct: 253 GYGRGSKVLGIPTANLSTEGYSDVLSEHPSGVYFGWAGLSTRGIFKMVMSIGWNPFFDNV 312

Query: 301 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 360
           EKTIEPWLLH+FD DFY EDLRLVVVGYIRPEANFP+LESL+AKIHEDGRIAERALDLPL
Sbjct: 313 EKTIEPWLLHDFDGDFYGEDLRLVVVGYIRPEANFPSLESLIAKIHEDGRIAERALDLPL 372

Query: 361 YSKYRNEQYLKEHDA 376
           YSKYRN+QYLK+ DA
Sbjct: 373 YSKYRNDQYLKQRDA 386

[Close]

BLAST of MS006120 vs. NCBI nr
Match: XP_008442000.1 (PREDICTED: bifunctional riboflavin kinase/FMN phosphatase [Cucumis melo])

HSP 1 Score: 703.4 bits (1814), Expect = 1.1e-198
Identity = 340/375 (90.67%), Postives = 357/375 (95.20%), Query Frame = 0


Query: 1   MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 60
           M+VSGVILDLDGTLL+T DGIVN+VLKSFLGKYGKQWDGREA R+ GKTP+ESAAVIIED
Sbjct: 13  MVVSGVILDLDGTLLHT-DGIVNDVLKSFLGKYGKQWDGREALRISGKTPYESAAVIIED 72

Query: 61  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 120
           YGL C+SAELMSQISPLFAERWCNIKALPGANRLIKHF+ HRVPIALASNS+RENIE+KI
Sbjct: 73  YGLPCSSAELMSQISPLFAERWCNIKALPGANRLIKHFSSHRVPIALASNSSRENIESKI 132

Query: 121 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 180
           SFHPGWKDSFSVIIG DEV   KPSPEIFLESAKRLNLEPS CLVIEDSVPGV AGKAAG
Sbjct: 133 SFHPGWKDSFSVIIGGDEVTAAKPSPEIFLESAKRLNLEPSSCLVIEDSVPGVAAGKAAG 192

Query: 181 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 240
           MKVVAVPSL KKSHLYSSADEVINSLLDFQP+KWGLPPFEDWVENTLPINP YIGGPVVK
Sbjct: 193 MKVVAVPSLPKKSHLYSSADEVINSLLDFQPQKWGLPPFEDWVENTLPINPWYIGGPVVK 252

Query: 241 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 300
           GYGRGSKVLGIPTANLS EGYSDVLSEHPSGVYFGWAG+S RG+FKMVMSIGWNPFFDN 
Sbjct: 253 GYGRGSKVLGIPTANLSTEGYSDVLSEHPSGVYFGWAGVSTRGIFKMVMSIGWNPFFDNA 312

Query: 301 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 360
           EKTIEPWLLH+FDDDFY EDLRLVVVGYIRPEANFP+LESL+AKI EDGRIAERALDLPL
Sbjct: 313 EKTIEPWLLHDFDDDFYGEDLRLVVVGYIRPEANFPSLESLIAKIQEDGRIAERALDLPL 372

Query: 361 YSKYRNEQYLKEHDA 376
           YSKYRN+QYLK+HDA
Sbjct: 373 YSKYRNDQYLKQHDA 386

[Close]

BLAST of MS006120 vs. NCBI nr
Match: KAA0056944.1 (bifunctional riboflavin kinase/FMN phosphatase [Cucumis melo var. makuwa] >TYK26371.1 bifunctional riboflavin kinase/FMN phosphatase [Cucumis melo var. makuwa])

HSP 1 Score: 703.4 bits (1814), Expect = 1.1e-198
Identity = 340/375 (90.67%), Postives = 357/375 (95.20%), Query Frame = 0


Query: 1   MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 60
           M+VSGVILDLDGTLL+T DGIVN+VLKSFLGKYGKQWDGREA R+ GKTP+ESAAVIIED
Sbjct: 1   MVVSGVILDLDGTLLHT-DGIVNDVLKSFLGKYGKQWDGREALRISGKTPYESAAVIIED 60

Query: 61  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 120
           YGL C+SAELMSQISPLFAERWCNIKALPGANRLIKHF+ HRVPIALASNS+RENIE+KI
Sbjct: 61  YGLPCSSAELMSQISPLFAERWCNIKALPGANRLIKHFSSHRVPIALASNSSRENIESKI 120

Query: 121 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 180
           SFHPGWKDSFSVIIG DEV   KPSPEIFLESAKRLNLEPS CLVIEDSVPGV AGKAAG
Sbjct: 121 SFHPGWKDSFSVIIGGDEVTAAKPSPEIFLESAKRLNLEPSSCLVIEDSVPGVAAGKAAG 180

Query: 181 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 240
           MKVVAVPSL KKSHLYSSADEVINSLLDFQP+KWGLPPFEDWVENTLPINP YIGGPVVK
Sbjct: 181 MKVVAVPSLPKKSHLYSSADEVINSLLDFQPQKWGLPPFEDWVENTLPINPWYIGGPVVK 240

Query: 241 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 300
           GYGRGSKVLGIPTANLS EGYSDVLSEHPSGVYFGWAG+S RG+FKMVMSIGWNPFFDN 
Sbjct: 241 GYGRGSKVLGIPTANLSTEGYSDVLSEHPSGVYFGWAGVSTRGIFKMVMSIGWNPFFDNA 300

Query: 301 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 360
           EKTIEPWLLH+FDDDFY EDLRLVVVGYIRPEANFP+LESL+AKI EDGRIAERALDLPL
Sbjct: 301 EKTIEPWLLHDFDDDFYGEDLRLVVVGYIRPEANFPSLESLIAKIQEDGRIAERALDLPL 360

Query: 361 YSKYRNEQYLKEHDA 376
           YSKYRN+QYLK+HDA
Sbjct: 361 YSKYRNDQYLKQHDA 374

[Close]

BLAST of MS006120 vs. ExPASy Swiss-Prot
Match: Q84MD8 (Bifunctional riboflavin kinase/FMN phosphatase OS=Arabidopsis thaliana OX=3702 GN=FHY PE=1 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.1e-156
Identity = 257/367 (70.03%), Postives = 314/367 (85.56%), Query Frame = 0


Query: 4   SGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIEDYGL 63
           S V++DLDGTL+NT DG+V ++L+ +L KYGKQWDGRE+ ++VGKTP E+A  I+EDY L
Sbjct: 12  SCVLIDLDGTLINT-DGVVGDILRKYLCKYGKQWDGRESLKIVGKTPVEAATTIVEDYEL 71

Query: 64  SCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKISFH 123
            C   E  S+  PLF+ +   IK+LPGANRLI+H   H VP+ALASNS+R NIE+KIS+H
Sbjct: 72  PCKVDEFNSEFYPLFSAQMDKIKSLPGANRLIRHLKCHGVPVALASNSSRANIESKISYH 131

Query: 124 PGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAGMKV 183
            GWK+ FSVI+GSDEV  GKPSP+IFLE+AKRL  +P+ CLVIEDSVPGV AGKAAG KV
Sbjct: 132 EGWKECFSVIVGSDEVSKGKPSPDIFLEAAKRLKKDPADCLVIEDSVPGVMAGKAAGTKV 191

Query: 184 VAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVKGYG 243
           +AVPSL K++HLY+SADEVINSLLD + EKWGLPPF+DW+ENTLPI+P +IGGPV+KG+G
Sbjct: 192 IAVPSLPKQTHLYTSADEVINSLLDIRLEKWGLPPFQDWIENTLPIDPWHIGGPVIKGFG 251

Query: 244 RGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNKEKT 303
           RGSKVLGIPTANLS + Y+D L EHPSGVYFGWAGL+ RGVFKMVMSIGWNP+F+NKEKT
Sbjct: 252 RGSKVLGIPTANLSTKDYADELVEHPSGVYFGWAGLAKRGVFKMVMSIGWNPYFNNKEKT 311

Query: 304 IEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPLYSK 363
           IEPWLLH+F +DFY E+LRL++VGYIRPEANF +LESL+AKIHED  +AE+ALDLP Y+K
Sbjct: 312 IEPWLLHDFTEDFYGEELRLIIVGYIRPEANFSSLESLIAKIHEDREVAEKALDLPSYAK 371

Query: 364 YRNEQYL 371
           ++ + YL
Sbjct: 372 FKGDPYL 377

[Close]

BLAST of MS006120 vs. ExPASy Swiss-Prot
Match: O74866 (Riboflavin kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fmn1 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.3e-32
Identity = 67/141 (47.52%), Postives = 95/141 (67.38%), Query Frame = 0


Query: 231 PLYIGGPVVKGYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMS 290
           P+   G VV G+GRGSK LGIPTAN+S +   ++L    SGVYFG+A +  R VF MVMS
Sbjct: 23  PIRFEGKVVHGFGRGSKELGIPTANISEDAIQELLRYRDSGVYFGYAMVQKR-VFPMVMS 82

Query: 291 IGWNPFFDNKEKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGR 350
           +GWNP++ NK ++ E  L+    +DFY E +R++V+GYIRPE N+  L+ L+  IH D R
Sbjct: 83  VGWNPYYKNKLRSAEVHLIERQGEDFYEEIMRVIVLGYIRPELNYAGLDKLIEDIHTDIR 142

Query: 351 IAERALDLPLYSKYRNEQYLK 372
           +A  ++D P YS Y+ + + K
Sbjct: 143 VALNSMDRPSYSSYKKDPFFK 162

[Close]

BLAST of MS006120 vs. ExPASy Swiss-Prot
Match: O76206 (Putative riboflavin kinase OS=Drosophila melanogaster OX=7227 GN=anon-84Ea PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.6e-32
Identity = 66/142 (46.48%), Postives = 94/142 (66.20%), Query Frame = 0


Query: 231 PLYIGGPVVKGYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMS 290
           PL+ GG +V+G+GRGSK LGIPTAN   E    +    P+G Y+GWA +    V KMV+S
Sbjct: 6   PLFAGGEIVRGFGRGSKELGIPTANFPLEVVKSLPESLPTGAYYGWANVDNGPVHKMVLS 65

Query: 291 IGWNPFFDNKEKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGR 350
           IGWNPF++NKEK++E  +LH+F+ D Y + L++ +VGY+RPE +F +LESL+A I  D  
Sbjct: 66  IGWNPFYNNKEKSVETHMLHDFNCDLYGQTLKICIVGYLRPERSFDSLESLIAAIRGDIE 125

Query: 351 IAERALDLPLYSKYRNEQYLKE 373
            A+  LD    +K +   +  E
Sbjct: 126 QAKAFLDEADKAKLKEAPFFTE 147

[Close]

BLAST of MS006120 vs. ExPASy Swiss-Prot
Match: Q8CFV9 (Riboflavin kinase OS=Mus musculus OX=10090 GN=Rfk PE=1 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 3.7e-32
Identity = 62/141 (43.97%), Postives = 97/141 (68.79%), Query Frame = 0


Query: 231 PLYIGGPVVKGYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMS 290
           P +  G VV+G+GRGSK LGIPTAN   +   ++ ++  +G+Y+GWA +    V KMV+S
Sbjct: 5   PFFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADVSTGIYYGWASVGSGDVHKMVVS 64

Query: 291 IGWNPFFDNKEKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGR 350
           IGWNP++ N +K++E  ++H F +DFY E L + +VGY+RPE NF +LESL++ I  D  
Sbjct: 65  IGWNPYYKNVKKSMETHIIHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIE 124

Query: 351 IAERALDLPLYSKYRNEQYLK 372
            A++ LDLP + K +++ + +
Sbjct: 125 EAKKQLDLPEHLKLKDDNFFQ 145

[Close]

BLAST of MS006120 vs. ExPASy Swiss-Prot
Match: Q08623 (Pseudouridine-5'-phosphatase OS=Homo sapiens OX=9606 GN=PUDP PE=1 SV=3)

HSP 1 Score: 139.8 bits (351), Expect = 6.2e-32
Identity = 85/222 (38.29%), Postives = 126/222 (56.76%), Query Frame = 0


Query: 3   VSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIEDYG 62
           V+ +I D+DG LL+T + + + V +    +Y K++       V+GK   E+A +II+   
Sbjct: 8   VTHLIFDMDGLLLDT-ERLYSVVFQEICNRYDKKYSWDVKSLVMGKKALEAAQIIIDVLQ 67

Query: 63  LSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKISF 122
           L  +  EL+ +      E +     +PGA +LI H   H +P ALA++S   + + K S 
Sbjct: 68  LPMSKEELVEESQTKLKEVFPTAALMPGAEKLIIHLRKHGIPFALATSSGSASFDMKTSR 127

Query: 123 HPGWKDSFS-VIIGSD-EVRTGKPSPEIFLESAKRLNLEPS--RCLVIEDSVPGVTAGKA 182
           H  +   FS +++G D EV+ GKP P+IFL  AKR +  P+  +CLV ED+  GV A  A
Sbjct: 128 HKEFFSLFSHIVLGDDPEVQHGKPDPDIFLACAKRFSPPPAMEKCLVFEDAPNGVEAALA 187

Query: 183 AGMKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFE 221
           AGM+VV VP       L + A  V+NSL DFQPE +GLP +E
Sbjct: 188 AGMQVVMVPDGNLSRDLTTKATLVLNSLQDFQPELFGLPSYE 228

[Close]

BLAST of MS006120 vs. ExPASy TrEMBL
Match: A0A6J1CFG7 (Riboflavin kinase OS=Momordica charantia OX=3673 GN=LOC111010948 PE=4 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 3.8e-218
Identity = 375/375 (100.00%), Postives = 375/375 (100.00%), Query Frame = 0


Query: 1   MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 60
           MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED
Sbjct: 12  MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 71

Query: 61  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 120
           YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI
Sbjct: 72  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 131

Query: 121 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 180
           SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG
Sbjct: 132 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 191

Query: 181 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 240
           MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK
Sbjct: 192 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 251

Query: 241 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 300
           GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK
Sbjct: 252 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 311

Query: 301 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 360
           EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL
Sbjct: 312 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 371

Query: 361 YSKYRNEQYLKEHDA 376
           YSKYRNEQYLKEHDA
Sbjct: 372 YSKYRNEQYLKEHDA 386

[Close]

BLAST of MS006120 vs. ExPASy TrEMBL
Match: A0A6J1CG93 (Riboflavin kinase OS=Momordica charantia OX=3673 GN=LOC111010948 PE=4 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 3.6e-216
Identity = 374/375 (99.73%), Postives = 374/375 (99.73%), Query Frame = 0


Query: 1   MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 60
           MIVSGVILDLDGTLLNT DGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED
Sbjct: 12  MIVSGVILDLDGTLLNT-DGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 71

Query: 61  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 120
           YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI
Sbjct: 72  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 131

Query: 121 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 180
           SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG
Sbjct: 132 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 191

Query: 181 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 240
           MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK
Sbjct: 192 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 251

Query: 241 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 300
           GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK
Sbjct: 252 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 311

Query: 301 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 360
           EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL
Sbjct: 312 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 371

Query: 361 YSKYRNEQYLKEHDA 376
           YSKYRNEQYLKEHDA
Sbjct: 372 YSKYRNEQYLKEHDA 385

[Close]

BLAST of MS006120 vs. ExPASy TrEMBL
Match: A0A0A0LRT8 (Riboflavin kinase OS=Cucumis sativus OX=3659 GN=Csa_1G071920 PE=4 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 3.1e-199
Identity = 341/375 (90.93%), Postives = 358/375 (95.47%), Query Frame = 0


Query: 1   MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 60
           M+VSGVILDLDGTLL+T DGIVN+VLKSFLGKYGKQWDGREA RV GKTP+ESAAVI+ED
Sbjct: 13  MVVSGVILDLDGTLLHT-DGIVNDVLKSFLGKYGKQWDGREALRVTGKTPYESAAVIVED 72

Query: 61  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 120
           YGL C+SAELMSQISPLFAERWCNIKALPGANRLIKHF+ HRVPIALASNS+RENIE+KI
Sbjct: 73  YGLPCSSAELMSQISPLFAERWCNIKALPGANRLIKHFSNHRVPIALASNSSRENIESKI 132

Query: 121 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 180
           SFHPGWKDSFSVIIGS+EV   KPSPEIFLESAKRLNLEPS CLVIEDSVPGV AGKAAG
Sbjct: 133 SFHPGWKDSFSVIIGSNEVTAAKPSPEIFLESAKRLNLEPSSCLVIEDSVPGVAAGKAAG 192

Query: 181 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 240
           MKVVAVPSL KKSHLYSSADEVINSLLDFQP+KWGLPPFEDWVENTLPINPLYIGGPVVK
Sbjct: 193 MKVVAVPSLPKKSHLYSSADEVINSLLDFQPQKWGLPPFEDWVENTLPINPLYIGGPVVK 252

Query: 241 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 300
           GYGRGSKVLGIPTANLS EGYSDVLSEHPSGVYFGWAGLS RG+FKMVMSIGWNPFFDN 
Sbjct: 253 GYGRGSKVLGIPTANLSTEGYSDVLSEHPSGVYFGWAGLSTRGIFKMVMSIGWNPFFDNV 312

Query: 301 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 360
           EKTIEPWLLH+FD DFY EDLRLVVVGYIRPEANFP+LESL+AKIHEDGRIAERALDLPL
Sbjct: 313 EKTIEPWLLHDFDGDFYGEDLRLVVVGYIRPEANFPSLESLIAKIHEDGRIAERALDLPL 372

Query: 361 YSKYRNEQYLKEHDA 376
           YSKYRN+QYLK+ DA
Sbjct: 373 YSKYRNDQYLKQRDA 386

[Close]

BLAST of MS006120 vs. ExPASy TrEMBL
Match: A0A5D3DRR7 (Riboflavin kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00320 PE=4 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 5.2e-199
Identity = 340/375 (90.67%), Postives = 357/375 (95.20%), Query Frame = 0


Query: 1   MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 60
           M+VSGVILDLDGTLL+T DGIVN+VLKSFLGKYGKQWDGREA R+ GKTP+ESAAVIIED
Sbjct: 1   MVVSGVILDLDGTLLHT-DGIVNDVLKSFLGKYGKQWDGREALRISGKTPYESAAVIIED 60

Query: 61  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 120
           YGL C+SAELMSQISPLFAERWCNIKALPGANRLIKHF+ HRVPIALASNS+RENIE+KI
Sbjct: 61  YGLPCSSAELMSQISPLFAERWCNIKALPGANRLIKHFSSHRVPIALASNSSRENIESKI 120

Query: 121 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 180
           SFHPGWKDSFSVIIG DEV   KPSPEIFLESAKRLNLEPS CLVIEDSVPGV AGKAAG
Sbjct: 121 SFHPGWKDSFSVIIGGDEVTAAKPSPEIFLESAKRLNLEPSSCLVIEDSVPGVAAGKAAG 180

Query: 181 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 240
           MKVVAVPSL KKSHLYSSADEVINSLLDFQP+KWGLPPFEDWVENTLPINP YIGGPVVK
Sbjct: 181 MKVVAVPSLPKKSHLYSSADEVINSLLDFQPQKWGLPPFEDWVENTLPINPWYIGGPVVK 240

Query: 241 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 300
           GYGRGSKVLGIPTANLS EGYSDVLSEHPSGVYFGWAG+S RG+FKMVMSIGWNPFFDN 
Sbjct: 241 GYGRGSKVLGIPTANLSTEGYSDVLSEHPSGVYFGWAGVSTRGIFKMVMSIGWNPFFDNA 300

Query: 301 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 360
           EKTIEPWLLH+FDDDFY EDLRLVVVGYIRPEANFP+LESL+AKI EDGRIAERALDLPL
Sbjct: 301 EKTIEPWLLHDFDDDFYGEDLRLVVVGYIRPEANFPSLESLIAKIQEDGRIAERALDLPL 360

Query: 361 YSKYRNEQYLKEHDA 376
           YSKYRN+QYLK+HDA
Sbjct: 361 YSKYRNDQYLKQHDA 374

[Close]

BLAST of MS006120 vs. ExPASy TrEMBL
Match: A0A1S3B5C1 (Riboflavin kinase OS=Cucumis melo OX=3656 GN=LOC103485993 PE=4 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 5.2e-199
Identity = 340/375 (90.67%), Postives = 357/375 (95.20%), Query Frame = 0


Query: 1   MIVSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIED 60
           M+VSGVILDLDGTLL+T DGIVN+VLKSFLGKYGKQWDGREA R+ GKTP+ESAAVIIED
Sbjct: 13  MVVSGVILDLDGTLLHT-DGIVNDVLKSFLGKYGKQWDGREALRISGKTPYESAAVIIED 72

Query: 61  YGLSCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 120
           YGL C+SAELMSQISPLFAERWCNIKALPGANRLIKHF+ HRVPIALASNS+RENIE+KI
Sbjct: 73  YGLPCSSAELMSQISPLFAERWCNIKALPGANRLIKHFSSHRVPIALASNSSRENIESKI 132

Query: 121 SFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAG 180
           SFHPGWKDSFSVIIG DEV   KPSPEIFLESAKRLNLEPS CLVIEDSVPGV AGKAAG
Sbjct: 133 SFHPGWKDSFSVIIGGDEVTAAKPSPEIFLESAKRLNLEPSSCLVIEDSVPGVAAGKAAG 192

Query: 181 MKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVK 240
           MKVVAVPSL KKSHLYSSADEVINSLLDFQP+KWGLPPFEDWVENTLPINP YIGGPVVK
Sbjct: 193 MKVVAVPSLPKKSHLYSSADEVINSLLDFQPQKWGLPPFEDWVENTLPINPWYIGGPVVK 252

Query: 241 GYGRGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNK 300
           GYGRGSKVLGIPTANLS EGYSDVLSEHPSGVYFGWAG+S RG+FKMVMSIGWNPFFDN 
Sbjct: 253 GYGRGSKVLGIPTANLSTEGYSDVLSEHPSGVYFGWAGVSTRGIFKMVMSIGWNPFFDNA 312

Query: 301 EKTIEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPL 360
           EKTIEPWLLH+FDDDFY EDLRLVVVGYIRPEANFP+LESL+AKI EDGRIAERALDLPL
Sbjct: 313 EKTIEPWLLHDFDDDFYGEDLRLVVVGYIRPEANFPSLESLIAKIQEDGRIAERALDLPL 372

Query: 361 YSKYRNEQYLKEHDA 376
           YSKYRN+QYLK+HDA
Sbjct: 373 YSKYRNDQYLKQHDA 386

[Close]

BLAST of MS006120 vs. TAIR 10
Match: AT4G21470.1 (riboflavin kinase/FMN hydrolase )

HSP 1 Score: 554.3 bits (1427), Expect = 7.5e-158
Identity = 257/367 (70.03%), Postives = 314/367 (85.56%), Query Frame = 0


Query: 4   SGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIEDYGL 63
           S V++DLDGTL+NT DG+V ++L+ +L KYGKQWDGRE+ ++VGKTP E+A  I+EDY L
Sbjct: 12  SCVLIDLDGTLINT-DGVVGDILRKYLCKYGKQWDGRESLKIVGKTPVEAATTIVEDYEL 71

Query: 64  SCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKISFH 123
            C   E  S+  PLF+ +   IK+LPGANRLI+H   H VP+ALASNS+R NIE+KIS+H
Sbjct: 72  PCKVDEFNSEFYPLFSAQMDKIKSLPGANRLIRHLKCHGVPVALASNSSRANIESKISYH 131

Query: 124 PGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAGMKV 183
            GWK+ FSVI+GSDEV  GKPSP+IFLE+AKRL  +P+ CLVIEDSVPGV AGKAAG KV
Sbjct: 132 EGWKECFSVIVGSDEVSKGKPSPDIFLEAAKRLKKDPADCLVIEDSVPGVMAGKAAGTKV 191

Query: 184 VAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFEDWVENTLPINPLYIGGPVVKGYG 243
           +AVPSL K++HLY+SADEVINSLLD + EKWGLPPF+DW+ENTLPI+P +IGGPV+KG+G
Sbjct: 192 IAVPSLPKQTHLYTSADEVINSLLDIRLEKWGLPPFQDWIENTLPIDPWHIGGPVIKGFG 251

Query: 244 RGSKVLGIPTANLSAEGYSDVLSEHPSGVYFGWAGLSMRGVFKMVMSIGWNPFFDNKEKT 303
           RGSKVLGIPTANLS + Y+D L EHPSGVYFGWAGL+ RGVFKMVMSIGWNP+F+NKEKT
Sbjct: 252 RGSKVLGIPTANLSTKDYADELVEHPSGVYFGWAGLAKRGVFKMVMSIGWNPYFNNKEKT 311

Query: 304 IEPWLLHEFDDDFYNEDLRLVVVGYIRPEANFPTLESLVAKIHEDGRIAERALDLPLYSK 363
           IEPWLLH+F +DFY E+LRL++VGYIRPEANF +LESL+AKIHED  +AE+ALDLP Y+K
Sbjct: 312 IEPWLLHDFTEDFYGEELRLIIVGYIRPEANFSSLESLIAKIHEDREVAEKALDLPSYAK 371

Query: 364 YRNEQYL 371
           ++ + YL
Sbjct: 372 FKGDPYL 377

[Close]

BLAST of MS006120 vs. TAIR 10
Match: AT5G57440.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 136.0 bits (341), Expect = 6.4e-32
Identity = 79/229 (34.50%), Postives = 133/229 (58.08%), Query Frame = 0


Query: 3   VSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIEDYG 62
           ++ VI D+DG LL+T +    EV +  L ++ K++D     +++G+   E+A + +E+ G
Sbjct: 14  ITHVIFDMDGLLLDT-EKFYTEVQEIILARFNKKFDWSLKAKMMGRKAIEAARIFVEESG 73

Query: 63  L--SCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 122
           +  S ++ + + +   +  + +   + +PGA+RLIKH     +PI +A+ +   + + K 
Sbjct: 74  ISDSLSAEDFLVERESMLQDLFPTSELMPGASRLIKHLHVKNIPICIATGTHTRHYDLKT 133

Query: 123 SFHPGWKDSFS----VIIGSD-EVRTGKPSPEIFLESAKRLN---LEPSRCLVIEDSVPG 182
             H   ++ FS    V+ G D EV+ GKP+P+ FL +A+R     ++  + LV ED+  G
Sbjct: 134 QRH---RELFSLMHHVVRGDDPEVKQGKPAPDGFLAAARRFKDGPVDSQKVLVFEDAPSG 193

Query: 183 VTAGKAAGMKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFED 222
           V A K AGM VV VP  +        AD++I SL+DF+PE+WGLPPFED
Sbjct: 194 VLAAKNAGMNVVMVPDPRLDISHQDVADQIITSLVDFKPEEWGLPPFED 238

[Close]

BLAST of MS006120 vs. TAIR 10
Match: AT4G25840.1 (glycerol-3-phosphatase 1 )

HSP 1 Score: 134.4 bits (337), Expect = 1.9e-31
Identity = 76/229 (33.19%), Postives = 133/229 (58.08%), Query Frame = 0


Query: 3   VSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQWDGREAQRVVGKTPFESAAVIIEDYG 62
           ++ VI D+DG LL+T +    EV +  L +Y K +D     +++G+   E+A + +++ G
Sbjct: 71  ITHVIFDMDGLLLDT-EKFYTEVQEKILARYNKTFDWSLKAKMMGRKAIEAARLFVDESG 130

Query: 63  L--SCTSAELMSQISPLFAERWCNIKALPGANRLIKHFTGHRVPIALASNSTRENIETKI 122
           +  S ++ + + +   +  + +     +PGA+RL++H  G  +PI +A+ +   + + K 
Sbjct: 131 ISDSLSAEDFIVERESMLQDLFPTSDLMPGASRLLRHLHGKGIPICIATGTHTRHFDLKT 190

Query: 123 SFHPGWKDSFS----VIIGSD-EVRTGKPSPEIFLESAKRLN---LEPSRCLVIEDSVPG 182
             H   ++ FS    V+ G D EV+ GKP+P+ FL +++R     ++P + LV ED+  G
Sbjct: 191 QRH---RELFSLMHHVVRGDDPEVKEGKPAPDGFLAASRRFEDGPVDPRKVLVFEDAPSG 250

Query: 183 VTAGKAAGMKVVAVPSLQKKSHLYSSADEVINSLLDFQPEKWGLPPFED 222
           V A K AGM V+ VP  +      + AD+V+ SLLDF+PE+WGLP F+D
Sbjct: 251 VQAAKNAGMNVIMVPDSRLDKSYCNVADQVLASLLDFKPEEWGLPSFQD 295

[Close]

BLAST of MS006120 vs. TAIR 10
Match: AT1G56500.1 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 68.2 bits (165), Expect = 1.6e-11
Identity = 53/207 (25.60%), Postives = 97/207 (46.86%), Query Frame = 0


Query: 3   VSGVILDLDGTLLNTADGIVNEVLKSFLGKYGKQ--WDGREAQRVVGKTPF-ESAAVIIE 62
           VS V+ D+DG L N+ D +          + G +   D        G+  F    A + E
Sbjct: 74  VSAVLFDMDGVLCNSED-LSRRAAVDVFTEMGVEVTVDDFVPFMGTGEAKFLGGVASVKE 133

Query: 63  DYGLSCTSAELMSQISPLFAERWCNIKA---LPGANRLIKHFTGHRVPIALASNSTRENI 122
             G    +A+   +   ++ +++   ++    PGA  L+       + +A+AS++ R  +
Sbjct: 134 VKGFDPDAAK--ERFFEIYLDKYAKPESGIGFPGALELVTECKNKGLKVAVASSADRIKV 193

Query: 123 ETKISFHPGWKDSFSVIIGSDEVRTGKPSPEIFLESAKRLNLEPSRCLVIEDSVPGVTAG 182
           +  +         F  I+ +D     KP+P+IFL +AK L +  S C+VIED++ GV A 
Sbjct: 194 DANLKAAGLSLTMFDAIVSADAFENLKPAPDIFLAAAKILGVPTSECVVIEDALAGVQAA 253

Query: 183 KAAGMKVVAVPSLQKKSHLYSSADEVI 204
           +AA M+ +AV +   ++ L  +   +I
Sbjct: 254 QAANMRCIAVKTTLSEAILKDAGPSMI 277

[Close]

BLAST of MS006120 vs. TAIR 10
Match: AT3G48420.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 6.2e-11
Identity = 39/129 (30.23%), Postives = 66/129 (51.16%), Query Frame = 0


Query: 89  PGANRLIKHFTGHRVPIALASNSTRENIETKISFHPGWKDSFSV-IIGSDEVRTGKPSPE 148
           PG  +L+     + V +A+ S S  + +   +S   G + +  + I   D V   KP P 
Sbjct: 178 PGVAKLVDQALTNGVKVAVCSTSNEKAVSAIVSCLLGPERAEKIKIFAGDVVPKKKPDPA 237

Query: 149 IFLESAKRLNLEPSRCLVIEDSVPGVTAGKAAGMKVVAVPSLQKKSHLYSSADEVINSLL 208
           I+  +A+ L ++PS+C+V+EDS  G+ A KAAGM  +   S       + +AD V + + 
Sbjct: 238 IYNLAAETLGVDPSKCVVVEDSAIGLAAAKAAGMTCIVTKSGYTADEDFENADAVFDCIG 297

Query: 209 DFQPEKWGL 217
           D   E++ L
Sbjct: 298 DPPEERFDL 306

The following BLAST results are available for this feature:

BLAST of MS006120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140231.1	7.9e-218	100.00	bifunctional riboflavin kinase/FMN phosphatase isoform X1 [Momordica charantia]	[more]
XP_022140232.1	7.4e-216	99.73	bifunctional riboflavin kinase/FMN phosphatase isoform X2 [Momordica charantia]	[more]
XP_004146584.1	6.3e-199	90.93	bifunctional riboflavin kinase/FMN phosphatase [Cucumis sativus] >XP_011653006.1...	[more]
XP_008442000.1	1.1e-198	90.67	PREDICTED: bifunctional riboflavin kinase/FMN phosphatase [Cucumis melo]	[more]
KAA0056944.1	1.1e-198	90.67	bifunctional riboflavin kinase/FMN phosphatase [Cucumis melo var. makuwa] >TYK26...	[more]

BLAST of MS006120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84MD8	1.1e-156	70.03	Bifunctional riboflavin kinase/FMN phosphatase OS=Arabidopsis thaliana OX=3702 G...	[more]
O74866	1.3e-32	47.52	Riboflavin kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=2848...	[more]
O76206	1.6e-32	46.48	Putative riboflavin kinase OS=Drosophila melanogaster OX=7227 GN=anon-84Ea PE=2 ...	[more]
Q8CFV9	3.7e-32	43.97	Riboflavin kinase OS=Mus musculus OX=10090 GN=Rfk PE=1 SV=2	[more]
Q08623	6.2e-32	38.29	Pseudouridine-5'-phosphatase OS=Homo sapiens OX=9606 GN=PUDP PE=1 SV=3	[more]

BLAST of MS006120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CFG7	3.8e-218	100.00	Riboflavin kinase OS=Momordica charantia OX=3673 GN=LOC111010948 PE=4 SV=1	[more]
A0A6J1CG93	3.6e-216	99.73	Riboflavin kinase OS=Momordica charantia OX=3673 GN=LOC111010948 PE=4 SV=1	[more]
A0A0A0LRT8	3.1e-199	90.93	Riboflavin kinase OS=Cucumis sativus OX=3659 GN=Csa_1G071920 PE=4 SV=1	[more]
A0A5D3DRR7	5.2e-199	90.67	Riboflavin kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00...	[more]
A0A1S3B5C1	5.2e-199	90.67	Riboflavin kinase OS=Cucumis melo OX=3656 GN=LOC103485993 PE=4 SV=1	[more]

BLAST of MS006120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21470.1	7.5e-158	70.03	riboflavin kinase/FMN hydrolase	[more]
AT5G57440.1	6.4e-32	34.50	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]
AT4G25840.1	1.9e-31	33.19	glycerol-3-phosphatase 1	[more]
AT1G56500.1	1.6e-11	25.60	haloacid dehalogenase-like hydrolase family protein	[more]
AT3G48420.1	6.2e-11	30.23	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006439	HAD hydrolase, subfamily IA	PRINTS	PR00413	HADHALOGNASE	coord: 130..146 score: 37.75 coord: 3..14 score: 43.75 coord: 148..168 score: 17.46
IPR006439	HAD hydrolase, subfamily IA	TIGRFAM	TIGR01509	TIGR01509	coord: 125..186 e-value: 6.6E-15 score: 53.6
IPR015865	Riboflavin kinase domain, bacterial/eukaryotic	SMART	SM00904	Flavokinase_2	coord: 227..357 e-value: 5.4E-31 score: 119.0
IPR015865	Riboflavin kinase domain, bacterial/eukaryotic	PFAM	PF01687	Flavokinase	coord: 231..355 e-value: 8.7E-37 score: 126.0
IPR023214	HAD superfamily	GENE3D	3.40.50.1000		coord: 6..206 e-value: 4.8E-56 score: 191.7
IPR041492	Haloacid dehalogenase-like hydrolase	PFAM	PF13419	HAD_2	coord: 6..186 e-value: 2.7E-24 score: 86.2
IPR023198	Phosphoglycolate phosphatase-like, domain 2	GENE3D	1.10.150.240	Putative phosphatase; domain 2	coord: 17..85 e-value: 4.8E-56 score: 191.7
IPR023465	Riboflavin kinase domain superfamily	GENE3D	2.40.30.30		coord: 219..375 e-value: 2.8E-52 score: 178.6
IPR023465	Riboflavin kinase domain superfamily	SUPERFAMILY	82114	Riboflavin kinase-like	coord: 231..372
None	No IPR available	SFLD	SFLDG01129	C1.5:_HAD,_Beta-PGM,_Phosphat	coord: 5..209 e-value: 1.4E-38 score: 127.5
None	No IPR available	SFLD	SFLDG01135	C1.5.6:_HAD,_Beta-PGM,_Phosph	coord: 5..209 e-value: 1.4E-38 score: 127.5
None	No IPR available	PANTHER	PTHR22749:SF6	RIBOFLAVIN KINASE	coord: 3..373
IPR023468	Riboflavin kinase	PANTHER	PTHR22749	RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE	coord: 3..373
IPR036412	HAD-like superfamily	SUPERFAMILY	56784	HAD-like	coord: 4..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS006120.1	MS006120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009231	riboflavin biosynthetic process
molecular_function	GO:0008531	riboflavin kinase activity