Homology
BLAST of MS004994 vs. NCBI nr
Match:
XP_022140528.1 (Niemann-Pick C1 protein [Momordica charantia])
HSP 1 Score: 2273.0 bits (5889), Expect = 0.0e+00
Identity = 1158/1178 (98.30%), Postives = 1159/1178 (98.39%), Query Frame = 0
Query: 1 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 60
ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF
Sbjct: 49 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 108
Query: 61 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 120
DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY
Sbjct: 109 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 168
Query: 121 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 180
YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN
Sbjct: 169 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 228
Query: 181 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 240
FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL
Sbjct: 229 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 288
Query: 241 EVGCIDFSITIIYIIFISAFLGWALFHPARERRFSAREEPLLNIGDGHEISSVNLEENEY 300
E CIDFSITIIYIIFISAFLGWALFHPARERRFSAREEPLLNIGDGHEISSVNLEENEY
Sbjct: 289 ESSCIDFSITIIYIIFISAFLGWALFHPARERRFSAREEPLLNIGDGHEISSVNLEENEY 348
Query: 301 SATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLVR 360
SATK LTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLVR
Sbjct: 349 SATKEHGAQLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLVR 408
Query: 361 FKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDNI 420
FKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDNI
Sbjct: 409 FKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDNI 468
Query: 421 LLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQH 480
LLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQH
Sbjct: 469 LLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQH 528
Query: 481 AEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENG 540
AEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENG
Sbjct: 529 AEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENG 588
Query: 541 KAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFV 600
KAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFV
Sbjct: 589 KAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFV 648
Query: 601 YISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFL 660
YISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFL
Sbjct: 649 YISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFL 708
Query: 661 VLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMP 720
VLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMP
Sbjct: 709 VLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMP 768
Query: 721 ACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFN 780
ACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFN
Sbjct: 769 ACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFN 828
Query: 781 QGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDS 840
QGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDS
Sbjct: 829 QGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDS 888
Query: 841 YLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLTP 900
YLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLTP
Sbjct: 889 YLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLTP 948
Query: 901 ELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGVC 960
ELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGVC
Sbjct: 949 ELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGVC 1008
Query: 961 KDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGII 1020
KDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGII
Sbjct: 1009 KDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGII 1068
Query: 1021 KASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLDIWKIA 1080
KASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMD+FPYSVFYIFFEQYLDIWKIA
Sbjct: 1069 KASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDIFPYSVFYIFFEQYLDIWKIA 1128
Query: 1081 LINIAVALG-----------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLM 1140
LINIAVALG LWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLM
Sbjct: 1129 LINIAVALGAVFVVSLVITSCLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLM 1188
Query: 1141 SIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1168
SIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV
Sbjct: 1189 SIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1226
BLAST of MS004994 vs. NCBI nr
Match:
XP_038878641.1 (NPC intracellular cholesterol transporter 1-like [Benincasa hispida])
HSP 1 Score: 2092.8 bits (5421), Expect = 0.0e+00
Identity = 1059/1179 (89.82%), Postives = 1111/1179 (94.23%), Query Frame = 0
Query: 1 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 60
ERH+ EYCAMYDICGAR DGKVLNCPYGSPSVKPDELFS KIQSLCPTISGNVCCTEAQF
Sbjct: 50 ERHAAEYCAMYDICGARSDGKVLNCPYGSPSVKPDELFSAKIQSLCPTISGNVCCTEAQF 109
Query: 61 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 120
+TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCS RQSLFINVTSI+EVGG+MTVDGIDY
Sbjct: 110 ETLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSSRQSLFINVTSIAEVGGSMTVDGIDY 169
Query: 121 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 180
YVTEKFGKGLYDSCKDVKFGTMNTRA+DFVGGGAKSFEE FAFLG+K APGFPGSPY IN
Sbjct: 170 YVTEKFGKGLYDSCKDVKFGTMNTRALDFVGGGAKSFEEFFAFLGQKVAPGFPGSPYAIN 229
Query: 181 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 240
FK+N KSS MELMNVSVYSCGDTSLGCSCGDCP SPVCS LEPP PPKSNAC+I IW L
Sbjct: 230 FKVNPSKSSQMELMNVSVYSCGDTSLGCSCGDCPSSPVCSGLEPP-PPKSNACTINIWSL 289
Query: 241 EVGCIDFSITIIYIIFISAFLGWALFHPARE-RRFSAREEPLLNIGDGHEISSVNLEENE 300
++ CIDFSITI+Y+IFIS+FLGWALFHP RE RR SAR EPLLNIGD EI SVNLEENE
Sbjct: 290 KISCIDFSITILYVIFISSFLGWALFHPTRESRRSSARGEPLLNIGDDGEIQSVNLEENE 349
Query: 301 YSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLV 360
ATK LTLRNG+QLSTIQGYISNFYR YGAWVARNPILVL +SLSIVLILC+GLV
Sbjct: 350 NGATKEHGVHLTLRNGVQLSTIQGYISNFYREYGAWVARNPILVLCTSLSIVLILCMGLV 409
Query: 361 RFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDN 420
FKVET+PEKLWVGHGSKAAAEKQFFDS LAPFYRIEQ+IIATKP RHGRAPRIVTEDN
Sbjct: 410 CFKVETRPEKLWVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPGGRHGRAPRIVTEDN 469
Query: 421 ILLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQ 480
ILLLFDIQ+KVNELVANYSGS+VSLNDICLKPLGEDCATQSILQYFKM+PEN+DDYGGV+
Sbjct: 470 ILLLFDIQNKVNELVANYSGSVVSLNDICLKPLGEDCATQSILQYFKMNPENFDDYGGVE 529
Query: 481 HAEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNEN 540
HAEYCFQHY STETCFSAFKAPLDPSTSLGGF GNNYSEASAFVVTYPVNNA+D+VGNEN
Sbjct: 530 HAEYCFQHYTSTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAMDSVGNEN 589
Query: 541 GKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMF 600
GKA+AWEKAFVKL KEELLPLVQSRNLTLSFSSESSIEEELKRESTAD+LTI VSYLVMF
Sbjct: 590 GKAIAWEKAFVKLAKEELLPLVQSRNLTLSFSSESSIEEELKRESTADILTIAVSYLVMF 649
Query: 601 VYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPF 660
YISVALGDS+ISSSFYLSSKVLLGLSGVILV+LSVLGSVGFFSAIGVKSTLIIMEVIPF
Sbjct: 650 AYISVALGDSNISSSFYLSSKVLLGLSGVILVLLSVLGSVGFFSAIGVKSTLIIMEVIPF 709
Query: 661 LVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPM 720
LVLAVGVDNMCILVHAVKRQP+ELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPM
Sbjct: 710 LVLAVGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPM 769
Query: 721 PACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGF 780
PACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKV PHSDEPNQGF
Sbjct: 770 PACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVPPHSDEPNQGF 829
Query: 781 NQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRD 840
NQG+PGLLS YMKDVHAPLLGLWGVKI V+VIFVG TLG IALSTKIEVGLEQKIVLPRD
Sbjct: 830 NQGRPGLLSRYMKDVHAPLLGLWGVKIVVVVIFVGLTLGSIALSTKIEVGLEQKIVLPRD 889
Query: 841 SYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLT 900
SYLQDYFD+LAEYLRIGPP+YFVVKDYNYSS+SR TNQLCSISQCDSNSLLNEISRASLT
Sbjct: 890 SYLQDYFDELAEYLRIGPPVYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLT 949
Query: 901 PELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGV 960
PEL+YIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSS GV
Sbjct: 950 PELSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSEGV 1009
Query: 961 CKDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1020
CKDCTTCFH+SDL+ RPTT+QFQEKLPWFLNSLPSADCAKGGHGAYTNSVNL GYESGI
Sbjct: 1010 CKDCTTCFHHSDLIGDRPTTVQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLKGYESGI 1069
Query: 1021 IKASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLDIWKI 1080
IKASEFR+YHTPLNKQGDYVNALRAAKEFS+K+SDSLKMD+FPYSVFYIFFEQYLDIW+
Sbjct: 1070 IKASEFRSYHTPLNKQGDYVNALRAAKEFSAKISDSLKMDIFPYSVFYIFFEQYLDIWET 1129
Query: 1081 ALINIAVALG-----------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLL 1140
AL+NIA+ALG LWSS IIILVLAMIIIDLMG+MAIL IQLNAVSVVN+L
Sbjct: 1130 ALMNIAIALGAIFIVSLVITSSLWSSAIIILVLAMIIIDLMGVMAILKIQLNAVSVVNIL 1189
Query: 1141 MSIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1168
MSIGIAVEFCVH+VHAFLVS GDRSQRA++AL TMGASV
Sbjct: 1190 MSIGIAVEFCVHLVHAFLVSCGDRSQRAQQALSTMGASV 1227
BLAST of MS004994 vs. NCBI nr
Match:
XP_022970905.1 (Niemann-Pick C1 protein-like [Cucurbita maxima])
HSP 1 Score: 2082.0 bits (5393), Expect = 0.0e+00
Identity = 1053/1179 (89.31%), Postives = 1106/1179 (93.81%), Query Frame = 0
Query: 1 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 60
ERHS EYCAMYDICGAR DGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF
Sbjct: 49 ERHSEEYCAMYDICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 108
Query: 61 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 120
+TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSI+EVGG MTVDGIDY
Sbjct: 109 ETLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSIAEVGGKMTVDGIDY 168
Query: 121 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 180
+VTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEE FAFLG+K PG PGSPY+IN
Sbjct: 169 FVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEEFFAFLGQKVGPGIPGSPYSIN 228
Query: 181 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 240
FK+NT K S +ELMNVSVYSCGDTSLGCSCGDCP SP CSSLEPPSPPKSNAC+IRIW L
Sbjct: 229 FKVNTDKPSQVELMNVSVYSCGDTSLGCSCGDCPSSPACSSLEPPSPPKSNACTIRIWSL 288
Query: 241 EVGCIDFSITIIYIIFISAFLGWALFHPARER-RFSAREEPLLNIGDGHEISSVNLEENE 300
+ CIDFSITI+Y+IF+SAFLGWALFHP RER RFSAREEPLLNIGD E++SVNLEENE
Sbjct: 289 KSSCIDFSITILYVIFVSAFLGWALFHPTRERSRFSAREEPLLNIGDDGEVNSVNLEENE 348
Query: 301 YSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLV 360
ATK LTLRNG+QLSTIQGYI++FYRNYGAWVARNPILVL +SLSIVLILCVGLV
Sbjct: 349 NGATKEHGVHLTLRNGVQLSTIQGYIASFYRNYGAWVARNPILVLCTSLSIVLILCVGLV 408
Query: 361 RFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDN 420
RFKVET+PEKLWVGHGSKAAAEKQFFDS LAPFYRIEQ+IIATKP R APRIVTEDN
Sbjct: 409 RFKVETRPEKLWVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPSGRQSTAPRIVTEDN 468
Query: 421 ILLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQ 480
ILLLFDIQ+KVNELVANYSGS+VSL DICLKPLGEDCATQSILQYFKMDPEN+D+YGGV+
Sbjct: 469 ILLLFDIQNKVNELVANYSGSVVSLTDICLKPLGEDCATQSILQYFKMDPENFDEYGGVE 528
Query: 481 HAEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNEN 540
H EYC QHY STETCFSAFKAPLDPSTSLGGF GNNYSEASAFVVTYPVNNAID VG+EN
Sbjct: 529 HVEYCLQHYTSTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDDVGDEN 588
Query: 541 GKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMF 600
GKA+AWEKAFVKLVKEELLPLVQS+NLTLSFSSESSIEEELKRESTADVLTI VSYLVMF
Sbjct: 589 GKAIAWEKAFVKLVKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIAVSYLVMF 648
Query: 601 VYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPF 660
YISVALGDS+ISSSFYLSSKVLLGLSGV+LVVLSVLGSVGFFSA+GVKSTLIIMEVIPF
Sbjct: 649 AYISVALGDSNISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALGVKSTLIIMEVIPF 708
Query: 661 LVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPM 720
LVLAVGVDNMCILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVG FVPM
Sbjct: 709 LVLAVGVDNMCILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPM 768
Query: 721 PACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGF 780
PACRVFSMFAALAV LDFILQLSAFVALIVFDILRAE+HRVDCFPCIKV P SDEPNQGF
Sbjct: 769 PACRVFSMFAALAVFLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPPRSDEPNQGF 828
Query: 781 NQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRD 840
NQ + GLLS YMKDVHAPLLGLWGVKIAV++IFVG TLG I LSTKIEVGLEQKIVLPRD
Sbjct: 829 NQRRLGLLSRYMKDVHAPLLGLWGVKIAVVIIFVGLTLGSITLSTKIEVGLEQKIVLPRD 888
Query: 841 SYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLT 900
SYLQ YFDDLAEYLRIGPPLYFVVKDYNYSS+SR TNQLCSISQCDSNSLLNEISRASLT
Sbjct: 889 SYLQGYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLT 948
Query: 901 PELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGV 960
PELNYIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSYCPPDDQPPCC PDEG CD+S GV
Sbjct: 949 PELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCIPDEGFCDASEGV 1008
Query: 961 CKDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1020
C+DCTTCFH+SDLVAGRPTT+QF+EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI
Sbjct: 1009 CQDCTTCFHHSDLVAGRPTTVQFREKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1068
Query: 1021 IKASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLDIWKI 1080
IKASEFR+YHTPLNKQGDYVNALRAAKEF SK+SDSLKMD+FPYSVFYIFFEQYLDIWK
Sbjct: 1069 IKASEFRSYHTPLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKT 1128
Query: 1081 ALINIAVALG-----------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLL 1140
AL+NIA+ALG LWSSGIIILVLAMIIIDLMG+MAILNIQLNAVSVVN+L
Sbjct: 1129 ALMNIAIALGAIFIVSLVITSCLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNIL 1188
Query: 1141 MSIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1168
MSIGIAVEFCVH+VHAF VS GDRSQR++EAL TMGASV
Sbjct: 1189 MSIGIAVEFCVHLVHAFSVSCGDRSQRSQEALTTMGASV 1227
BLAST of MS004994 vs. NCBI nr
Match:
XP_022947380.1 (Niemann-Pick C1 protein-like [Cucurbita moschata])
HSP 1 Score: 2080.4 bits (5389), Expect = 0.0e+00
Identity = 1055/1179 (89.48%), Postives = 1103/1179 (93.55%), Query Frame = 0
Query: 1 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 60
ERHS EYCAMYDICGAR DGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF
Sbjct: 49 ERHSEEYCAMYDICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 108
Query: 61 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 120
+TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSI+EVGG MTVDGIDY
Sbjct: 109 ETLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSIAEVGGKMTVDGIDY 168
Query: 121 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 180
+VTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKS EE FAFLG+K PG PGSPY+IN
Sbjct: 169 FVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSSEEFFAFLGQKVGPGIPGSPYSIN 228
Query: 181 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 240
FK NT K S MELMNVSVYSCGDTSLGCSCGDCP SP CSSLEPPSPPKSNAC+IRIW L
Sbjct: 229 FKANTDKPSQMELMNVSVYSCGDTSLGCSCGDCPSSPACSSLEPPSPPKSNACTIRIWSL 288
Query: 241 EVGCIDFSITIIYIIFISAFLGWALFHPARE-RRFSAREEPLLNIGDGHEISSVNLEENE 300
+ CIDFSITI+Y+IFISAFLGWALFHP RE RRFSAREEPLLNIGD E++SVNLEENE
Sbjct: 289 KSSCIDFSITILYVIFISAFLGWALFHPTRERRRFSAREEPLLNIGDDGEVNSVNLEENE 348
Query: 301 YSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLV 360
ATK LTLRNG+QLSTIQGYI++FYRNYGAWVARNPILVL +SLSIVLILCVGLV
Sbjct: 349 NGATKEHGVHLTLRNGVQLSTIQGYIASFYRNYGAWVARNPILVLCTSLSIVLILCVGLV 408
Query: 361 RFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDN 420
RFKVET+PEKLWVGHGSKAAAEKQFFDS LAPFYRIEQ+IIATKP R APRIVTEDN
Sbjct: 409 RFKVETRPEKLWVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPSGRQSTAPRIVTEDN 468
Query: 421 ILLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQ 480
ILLLFDIQ+KVNELVANYSGS+VSL DICLKPLGEDCATQSILQYFKMDPEN+D+YGGV+
Sbjct: 469 ILLLFDIQNKVNELVANYSGSVVSLTDICLKPLGEDCATQSILQYFKMDPENFDEYGGVE 528
Query: 481 HAEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNEN 540
H EYC QHY STETCFSAFKAPLDPSTSLGGF GNNYSEASAFVVTYPVNNAID VG+EN
Sbjct: 529 HVEYCLQHYTSTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDDVGDEN 588
Query: 541 GKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMF 600
GKA+AWEKAFVKL KEELLPLVQS+NLTLSFSSESSIEEELKRESTADVLTI VSYLVMF
Sbjct: 589 GKAIAWEKAFVKLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIAVSYLVMF 648
Query: 601 VYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPF 660
YISVALGDS+ISSSFYLSSKVLLGLSGV+LVVLSVLGSVGFFSA+GVKSTLIIMEVIPF
Sbjct: 649 AYISVALGDSNISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALGVKSTLIIMEVIPF 708
Query: 661 LVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPM 720
LVLAVGVDNMCILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVG FVPM
Sbjct: 709 LVLAVGVDNMCILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPM 768
Query: 721 PACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGF 780
PACRVFSMFAALAV LDFILQLSAFVALIVFDI RAE+HRVDCFPCIKV P SDEPNQGF
Sbjct: 769 PACRVFSMFAALAVFLDFILQLSAFVALIVFDIRRAENHRVDCFPCIKVPPRSDEPNQGF 828
Query: 781 NQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRD 840
NQ + GLLS YMKDVHAPLLGLWGVKIAV++IFVG TLG IALSTKIEVGLEQKIVLPRD
Sbjct: 829 NQRRLGLLSRYMKDVHAPLLGLWGVKIAVVIIFVGLTLGSIALSTKIEVGLEQKIVLPRD 888
Query: 841 SYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLT 900
SYLQ YFDDLAEYLRIGPPLYFVVKDYNYSS+SR TNQLCSISQCDSNSLLNEISRASLT
Sbjct: 889 SYLQGYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLT 948
Query: 901 PELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGV 960
PELNYIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSYCPPDDQPPCC PDEG CD+S GV
Sbjct: 949 PELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCIPDEGFCDASEGV 1008
Query: 961 CKDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1020
C+DCTTCFH+SDLVAGRPTT+QF+EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI
Sbjct: 1009 CQDCTTCFHHSDLVAGRPTTVQFREKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1068
Query: 1021 IKASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLDIWKI 1080
IKASEFR+YHTPLNKQGDYVNALRAAKEF SK+SDSLKMD+FPYSVFYIFFEQYLDIWK
Sbjct: 1069 IKASEFRSYHTPLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKT 1128
Query: 1081 ALINIAVALG-----------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLL 1140
AL+NIA+ALG LWSSGIIILVLAMIIIDLMG+MAILNIQLNAVSVVN+L
Sbjct: 1129 ALMNIAIALGAIFIVSLVITSCLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNIL 1188
Query: 1141 MSIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1168
MSIGIAVEFCVH+VHAF VS GDRSQRAREAL TMGASV
Sbjct: 1189 MSIGIAVEFCVHLVHAFSVSCGDRSQRAREALTTMGASV 1227
BLAST of MS004994 vs. NCBI nr
Match:
KAG7035007.1 (Niemann-Pick C1 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 2076.6 bits (5379), Expect = 0.0e+00
Identity = 1054/1179 (89.40%), Postives = 1101/1179 (93.38%), Query Frame = 0
Query: 1 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 60
ERHS EYCAMYDICGAR DGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF
Sbjct: 8 ERHSEEYCAMYDICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 67
Query: 61 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 120
+TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSI+EVGG MTVDGIDY
Sbjct: 68 ETLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSIAEVGGKMTVDGIDY 127
Query: 121 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 180
+VTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKS EE FAFLG+K PG PGSPY+IN
Sbjct: 128 FVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSSEEFFAFLGQKVGPGIPGSPYSIN 187
Query: 181 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 240
FK NT K S MELMNVSVYSCGDTSLGCSCGDCP SP CSSLEPPSPPKSNAC+IRIW L
Sbjct: 188 FKANTDKPSQMELMNVSVYSCGDTSLGCSCGDCPSSPACSSLEPPSPPKSNACTIRIWSL 247
Query: 241 EVGCIDFSITIIYIIFISAFLGWALFHPARE-RRFSAREEPLLNIGDGHEISSVNLEENE 300
+ CIDFSITI+Y+IFISAFLGWALFHP RE RRFSAREEPLLNIGD E++SVNLEENE
Sbjct: 248 KSSCIDFSITILYVIFISAFLGWALFHPTRERRRFSAREEPLLNIGDDGEVNSVNLEENE 307
Query: 301 YSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLV 360
ATK LTLRNG+QLSTIQGYI++FYRNYGAWVARNPILVL +SLSIVLILCVGLV
Sbjct: 308 NGATKEHGVHLTLRNGVQLSTIQGYIASFYRNYGAWVARNPILVLCTSLSIVLILCVGLV 367
Query: 361 RFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDN 420
RFKVET+PEKLWVGHGSKAAAEKQFFDS LAPFYRIEQ+IIATKP R APRIVTEDN
Sbjct: 368 RFKVETRPEKLWVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPSGRQSTAPRIVTEDN 427
Query: 421 ILLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQ 480
ILLLFDIQ+KVNELVANYSGS+VSL DICLKPLGEDCATQSILQYFKMDPEN+D+YGGV+
Sbjct: 428 ILLLFDIQNKVNELVANYSGSVVSLTDICLKPLGEDCATQSILQYFKMDPENFDEYGGVE 487
Query: 481 HAEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNEN 540
H EYC QHY STETCFSAFKAPLDPSTSLGGF GNNYSEASAFVVTYPVNNAID VG+EN
Sbjct: 488 HVEYCLQHYTSTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDDVGDEN 547
Query: 541 GKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMF 600
GKA+AWEKAFVKL KEELLPLVQS+NLTLSFSSESSIEEELKRESTADVLTI VSYLVMF
Sbjct: 548 GKAIAWEKAFVKLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIAVSYLVMF 607
Query: 601 VYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPF 660
YISVALGDS+ISSSFYLSSKVLLGLSGV+LVVLSVLGSVGFFSA+GVKSTLIIMEVIPF
Sbjct: 608 AYISVALGDSNISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALGVKSTLIIMEVIPF 667
Query: 661 LVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPM 720
LVLAVGVDNMCILVHAVKRQPFELTLEERIS ALVEVGPSITLASLSEILAFAVG FVPM
Sbjct: 668 LVLAVGVDNMCILVHAVKRQPFELTLEERISHALVEVGPSITLASLSEILAFAVGTFVPM 727
Query: 721 PACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGF 780
PACRVFSMFAALAV LDFILQLSAFVALIVFDI RAE+HRVDCFPCIKV P SDE NQGF
Sbjct: 728 PACRVFSMFAALAVFLDFILQLSAFVALIVFDIRRAENHRVDCFPCIKVPPRSDEQNQGF 787
Query: 781 NQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRD 840
NQ + GLLS YMKDVHAPLLGLWGVKIAV++IFVG TLG IALSTKIEVGLEQKIVLPRD
Sbjct: 788 NQRRLGLLSRYMKDVHAPLLGLWGVKIAVVIIFVGLTLGSIALSTKIEVGLEQKIVLPRD 847
Query: 841 SYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLT 900
SYLQ YFDDLAEYLRIGPPLYFVVKDYNYSS+SR TNQLCSISQCDSNSLLNEISRASLT
Sbjct: 848 SYLQGYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLT 907
Query: 901 PELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGV 960
PELNYIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSYCPPDDQPPCC PDEG CD+S GV
Sbjct: 908 PELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCIPDEGFCDASEGV 967
Query: 961 CKDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1020
C+DCTTCFH+SDLVAGRPTT+QF+EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI
Sbjct: 968 CQDCTTCFHHSDLVAGRPTTVQFREKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1027
Query: 1021 IKASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLDIWKI 1080
IKASEFR+YHTPLNKQGDYVNALRAAKEF SK+SDSLKMD+FPYSVFYIFFEQYLDIWK
Sbjct: 1028 IKASEFRSYHTPLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKT 1087
Query: 1081 ALINIAVALG-----------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLL 1140
AL+NIA+ALG LWSSGIIILVLAMIIIDLMG+MAILNIQLNAVSVVN+L
Sbjct: 1088 ALMNIAIALGAIFIVSLVITSCLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNIL 1147
Query: 1141 MSIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1168
MSIGIAVEFCVH+VHAF VS GDRSQRAREAL TMGASV
Sbjct: 1148 MSIGIAVEFCVHLVHAFSVSCGDRSQRAREALTTMGASV 1186
BLAST of MS004994 vs. ExPASy Swiss-Prot
Match:
P56941 (NPC intracellular cholesterol transporter 1 OS=Sus scrofa OX=9823 GN=NPC1 PE=2 SV=1)
HSP 1 Score: 620.9 bits (1600), Expect = 2.9e-176
Identity = 426/1228 (34.69%), Postives = 646/1228 (52.61%), Query Frame = 0
Query: 5 TEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCP-TISGNV--CCTEAQFD 64
++ C Y CG K NC Y P E + +Q LCP GNV CC Q
Sbjct: 22 SQSCVWYGECGIASGDKRYNCRYSGPPKPLPEDGYDLVQELCPGFFFGNVSLCCDVQQLR 81
Query: 65 TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMT------V 124
TL+ +Q + CP+C N +NLFCEL+CSPRQS F+NVT+ + +T V
Sbjct: 82 TLKDNLQLPLQFLSRCPSCFYNLMNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNV 141
Query: 125 DGIDYYVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGS 184
++YYV E F +Y++C+DV+ + N +A+ + G ++ +
Sbjct: 142 KELEYYVGETFANAMYNACRDVEAPSSNEKALGLLCGREAQACNATNWIEYMFNKDNGQA 201
Query: 185 PYTINFKLNTPKSSPMELMNVSVYSCGDT----SLGCSCGDCPLSPVCSSLEPPSPPKSN 244
P+TI + + ME MN + C ++ + CSC DC S VC P PP
Sbjct: 202 PFTITPIFSDLPTHGMEPMNNATKGCDESVDEVTGPCSCQDC--SIVCGPKPQPPPPPVP 261
Query: 245 ACSIRIWFLEVGCIDFSITIIYIIFISAFLGWALFHPARERRFSAREEPLLNIGDGHEIS 304
+ + + V + + + F + F W R+R F + P+ DG+
Sbjct: 262 WRILGLDAMYVIMWSSYMAFLIVFFGAFFAVWCY----RKRYFVSEYTPI----DGNIAF 321
Query: 305 SVNLEE--NEYSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLS 364
SVN + + +G + + + + +GA+ R+P V+F SL+
Sbjct: 322 SVNSSDKGQAFCCDPLG------------AAFERGLRRLFAQWGAFCVRHPGCVVFFSLA 381
Query: 365 IVLILCVGLVRFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHG 424
++ GLV +V T P LW GS+A EK++FD+ PF+R+EQ+II + H
Sbjct: 382 FIVACSSGLVFIRVTTDPVDLWSAPGSQARREKEYFDTHFGPFFRMEQLIIRATNNQSHI 441
Query: 425 RAP----------RIVTEDNILLLFDIQDKVNELVANYSGSLVSLNDICLKPL---GEDC 484
P ++ D + + D+Q + + A+Y+ V+L DICL PL ++C
Sbjct: 442 YHPYPAGADVPFGPPLSRDILHQVLDLQTAIENITASYNNETVTLQDICLAPLSPYNKNC 501
Query: 485 ATQSILQYFKMDPENYDDYGG---------VQHAEYCFQHYAST-------ETCFSAFKA 544
S+L YF+ D G H YC + AS + C F
Sbjct: 502 TILSVLNYFQNSHSVLDHQVGDFFFVYADYHTHFLYCVRAPASLNDASLLHDPCLGTFGG 561
Query: 545 PLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENGKAVAWEKAFVKLVKEELLPL 604
P+ P LGG+ NY+ A+A V+T+PVNN + + +A AWE F+ VK P
Sbjct: 562 PVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDT-EKLQRAQAWESEFINFVKNYKNP- 621
Query: 605 VQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFVYISVALGDSSISSSFYLSSK 664
NLT+SF +E SIE+EL RES +D+ TI++SY +MF+YIS+ALG S + SK
Sbjct: 622 ----NLTISFMAERSIEDELNRESNSDLFTILISYAIMFLYISIALGHIKSCSRLLVDSK 681
Query: 665 VLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQP 724
+ LG++G+++V+ SV S+G FS IGV TLI++EVIPFLVLAVGVDN+ ILV +R
Sbjct: 682 ISLGIAGILIVLSSVACSLGIFSYIGVPLTLIVIEVIPFLVLAVGVDNIFILVQTYQRDE 741
Query: 725 --FELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMPACRVFSMFAALAVLLDFI 784
TL++++ L EV PS+ L+S SE +AF +G +PA FS+FA +AVL+DF+
Sbjct: 742 RLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGGLSVVPAVHTFSLFAGMAVLIDFL 801
Query: 785 LQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFNQGKPGLLSWYMKDVHAPL 844
LQ++ FV+L+ DI R E +R+D C++ E G Q L + K+ +APL
Sbjct: 802 LQITCFVSLLGLDIKRQEKNRLDVVCCVQ----GAEDGAGV-QASESCLFRFFKNSYAPL 861
Query: 845 LGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPP 904
L ++ VI +FVG IA+ K+E+GL+Q + +P DSY+ DYF L+ YL GPP
Sbjct: 862 LLKDWMRPIVIAVFVGVLSFSIAVLNKVEIGLDQSLSMPDDSYVMDYFQSLSRYLHAGPP 921
Query: 905 LYFVVKD-YNYSSESRQTNQLCSISQCDSNSLLNEISRASLTPELNYIAKPAASWLDDFL 964
+YFVV++ +NY+S Q N +C C+++SL+ +I A+ I +SW+DD+
Sbjct: 922 VYFVVEEGHNYTSLKGQ-NMVCGGLGCNNDSLVQQIFTAAQLDNYTRIGFAPSSWIDDYF 981
Query: 965 VWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGVCKDCTTCFHYSDLVAGRP 1024
W+ P + CCR + + + C++S V C C + RP
Sbjct: 982 DWIKPQS-SCCRVYNS---------------TDQFCNAS-VVDPTCIRCRPLTSEGKQRP 1041
Query: 1025 TTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRTYHTPLNKQGD 1084
F LP FL+ P+ C KGGH AY+++VN+ G SG + A+ F TYHT L D
Sbjct: 1042 QGEDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILGNGSG-VGATYFMTYHTVLQASAD 1101
Query: 1085 YVNALRAAKEFSSKMSDSLKMD-----VFPYSVFYIFFEQYLDIWKIALINIAVALG--- 1144
+++A++ A+ +S ++ ++ ++ VFPYSVFY+F+EQYL + + N+ V+LG
Sbjct: 1102 FIDAMQKARLIASNITRTMGLEASSYRVFPYSVFYVFYEQYLTVIDDTIFNLGVSLGAIF 1161
Query: 1145 ---------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLMSIGIAVEFCVH 1168
LW++ I+ + +AMI++++ G+M + I LNAVS+VNL+MS GI+VEFC H
Sbjct: 1162 LVTVVLMGCELWATVIMCVTIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSH 1197
BLAST of MS004994 vs. ExPASy Swiss-Prot
Match:
O35604 (NPC intracellular cholesterol transporter 1 OS=Mus musculus OX=10090 GN=Npc1 PE=1 SV=2)
HSP 1 Score: 619.8 bits (1597), Expect = 6.4e-176
Identity = 421/1227 (34.31%), Postives = 644/1227 (52.49%), Query Frame = 0
Query: 5 TEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTI---SGNVCCTEAQFD 64
++ C Y CG K NC Y P + + +Q LCP + + ++CC Q
Sbjct: 22 SQSCVWYGECGIATGDKRYNCKYSGPPKPLPKDGYDLVQELCPGLFFDNVSLCCDIQQLQ 81
Query: 65 TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMT------V 124
TL+S +Q + CP+C N + LFCEL+CSP QS F+NVT+ + T V
Sbjct: 82 TLKSNLQLPLQFLSRCPSCFYNLMTLFCELTCSPHQSQFLNVTATEDYFDPKTQENKTNV 141
Query: 125 DGIDYYVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGS 184
++Y+V + F +Y++C+DV+ + N +A+ + G ++ +
Sbjct: 142 KELEYFVGQSFANAMYNACRDVEAPSSNEKALGLLCGRDARACNATNWIEYMFNKDNGQA 201
Query: 185 PYTINFKLNTPKSSPMELMNVSVYSCGDT----SLGCSCGDCPLSPVCSSLEPPSPPKSN 244
P+TI + ME M + C ++ + CSC DC S VC P PP
Sbjct: 202 PFTIIPVFSDLSILGMEPMRNATKGCNESVDEVTGPCSCQDC--SIVCGPKPQPPPP--- 261
Query: 245 ACSIRIWFLEVGCIDFSITIIYIIFISAFLGWALFHPARERRFSAREEPLLNIGDGHEIS 304
RIW L+ + +T Y+ F+ F G L RR+ E + D +
Sbjct: 262 PMPWRIWGLDAMYVIMWVT--YVAFLFVFFGALLAVWCHRRRYFVSEYTPI---DSNIAF 321
Query: 305 SVNLEENEYSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIV 364
SVN + ++ L + + + +GA+ RNP ++F SL+ +
Sbjct: 322 SVNSSDKGEASCCDPLG----------AAFDDCLRRMFTKWGAFCVRNPTCIIFFSLAFI 381
Query: 365 LILCVGLVRFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRA 424
+ GLV +V T P +LW S+A EK++FD PF+R EQ+II H
Sbjct: 382 TVCSSGLVFVQVTTNPVELWSAPHSQARLEKEYFDKHFGPFFRTEQLIIQAPNTSVHIYE 441
Query: 425 P----------RIVTEDNILLLFDIQDKVNELVANYSGSLVSLNDICLKPL---GEDCAT 484
P + ++ + + D+Q + + A+Y+ V+L DIC+ PL ++C
Sbjct: 442 PYPAGADVPFGPPLNKEILHQVLDLQIAIESITASYNNETVTLQDICVAPLSPYNKNCTI 501
Query: 485 QSILQYFK-----MDPENYDDY----GGVQHAEYCFQHYASTE-------TCFSAFKAPL 544
S+L YF+ +D + DD+ H YC + AS C F P+
Sbjct: 502 MSVLNYFQNSHAVLDSQVGDDFYIYADYHTHFLYCVRAPASLNDTSLLHGPCLGTFGGPV 561
Query: 545 DPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENGKAVAWEKAFVKLVKEELLPLVQ 604
P LGG+ NY+ A+A V+T+PVNN + +A AWEK F+ VK P
Sbjct: 562 FPWLVLGGYDDQNYNNATALVITFPVNNYYNDT-ERLQRAWAWEKEFISFVKNYKNP--- 621
Query: 605 SRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFVYISVALGDSSISSSFYLSSKVL 664
NLT+SF++E SIE+EL RES +DV T+++SY+VMF+YIS+ALG S + SK+
Sbjct: 622 --NLTISFTAERSIEDELNRESNSDVFTVIISYVVMFLYISLALGHIQSCSRLLVDSKIS 681
Query: 665 LGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQP-- 724
LG++G+++V+ SV S+G FS +G+ TLI++EVIPFLVLAVGVDN+ ILV +R
Sbjct: 682 LGIAGILIVLSSVACSLGIFSYMGMPLTLIVIEVIPFLVLAVGVDNIFILVQTYQRDERL 741
Query: 725 FELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMPACRVFSMFAALAVLLDFILQ 784
E TL++++ L EV P++ L+S SE AF GA MPA FS+FA +AVL+DF+LQ
Sbjct: 742 QEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPAVHTFSLFAGMAVLIDFLLQ 801
Query: 785 LSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFNQGKPGLLSWYMKDVHAPLLG 844
++ FV+L+ DI R E + +D C++ + QG + + L ++ K+ APLL
Sbjct: 802 ITCFVSLLGLDIKRQEKNHLDILCCVR----GADDGQGSHASESYLFRFF-KNYFAPLLL 861
Query: 845 LWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLY 904
++ V+ +FVG +A+ K+++GL+Q + +P DSY+ DYF LA+YL GPP+Y
Sbjct: 862 KDWLRPIVVAVFVGVLSFSVAVVNKVDIGLDQSLSMPNDSYVIDYFKSLAQYLHSGPPVY 921
Query: 905 FVVKD-YNYSSESRQTNQLCSISQCDSNSLLNEISRASLTPELNYIAKPAASWLDDFLVW 964
FV+++ YNYSS Q N +C CD++SL+ +I A+ + +SW+DD+ W
Sbjct: 922 FVLEEGYNYSSRKGQ-NMVCGGMGCDNDSLVQQIFNAAELDTYTRVGFAPSSWIDDYFDW 981
Query: 965 LSPDAFGCCRKF-TNGSYCPPDDQPPCCFPDEGSCDSSGGVCKDCTTCFHYSDLVAGRPT 1024
+SP + CCR + +C P C C + RP
Sbjct: 982 VSPQS-SCCRLYNVTHQFCNASVMDP-----------------TCVRCRPLTPEGKQRPQ 1041
Query: 1025 TIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRTYHTPLNKQGDY 1084
+F + LP FL+ P+ C KGGH AY ++VN+ G + I A+ F TYHT L DY
Sbjct: 1042 GKEFMKFLPMFLSDNPNPKCGKGGHAAYGSAVNIVG-DDTYIGATYFMTYHTILKTSADY 1101
Query: 1085 VNALRAAKEFSSKMSDSLKMD-----VFPYSVFYIFFEQYLDIWKIALINIAVALG---- 1144
+A++ A+ +S ++++++ VFPYSVFY+F+EQYL I + N++V+LG
Sbjct: 1102 TDAMKKARLIASNITETMRSKGSDYRVFPYSVFYVFYEQYLTIIDDTIFNLSVSLGSIFL 1161
Query: 1145 --------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLMSIGIAVEFCVHI 1168
LWS+ I+ + +AMI++++ G+M + I LNAVS+VNL+MS GI+VEFC HI
Sbjct: 1162 VTLVVLGCELWSAVIMCITIAMILVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHI 1197
BLAST of MS004994 vs. ExPASy Swiss-Prot
Match:
O15118 (NPC intracellular cholesterol transporter 1 OS=Homo sapiens OX=9606 GN=NPC1 PE=1 SV=2)
HSP 1 Score: 617.8 bits (1592), Expect = 2.4e-175
Identity = 430/1234 (34.85%), Postives = 642/1234 (52.03%), Query Frame = 0
Query: 5 TEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCP-TISGNV--CCTEAQFD 64
++ C Y CG K NC Y P + + +Q LCP GNV CC Q
Sbjct: 22 SQSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQ 81
Query: 65 TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMT------V 124
TL+ +Q + CP+C N LNLFCEL+CSPRQS F+NVT+ + +T V
Sbjct: 82 TLKDNLQLPLQFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNV 141
Query: 125 DGIDYYVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGS 184
+ YYV + F +Y++C+DV+ + N +A+ + G ++ +
Sbjct: 142 KELQYYVGQSFANAMYNACRDVEAPSSNDKALGLLCGKDADACNATNWIEYMFNKDNGQA 201
Query: 185 PYTINFKLNTPKSSPMELMNVSVYSCGDT----SLGCSCGDCPLSPVCSSLEPPSPPKSN 244
P+TI + ME MN + C ++ + CSC DC S VC P PP +
Sbjct: 202 PFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDC--SIVCGPKPQPPPPPAP 261
Query: 245 ACSIRIW-FLEVGCIDFSITIIYIIFISAFLGWALFHP--ARERRFSAREEPLLNIGDGH 304
W L + + + I Y+ F+ F G A F R+R F + P+ D +
Sbjct: 262 ------WTILGLDAMYVIMWITYMAFLLVFFG-AFFAVWCYRKRYFVSEYTPI----DSN 321
Query: 305 EISSVNLEENEYSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSL 364
SVN + ++ D ++ + +G + + +G++ RNP V+F SL
Sbjct: 322 IAFSVNASDKGEASC---CDPVS-------AAFEGCLRRLFTRWGSFCVRNPGCVIFFSL 381
Query: 365 SIVLILCVGLVRFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERH 424
+ GLV +V T P LW S+A EK++FD PF+R EQ+II ++H
Sbjct: 382 VFITACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKH 441
Query: 425 GRAPRIVTED-------NILLL---FDIQDKVNELVANYSGSLVSLNDICLKPL---GED 484
P D +I +L D+Q + + A+Y V+L DICL PL +
Sbjct: 442 IYQPYPSGADVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTN 501
Query: 485 CATQSILQYFK-----MDPENYDDY----GGVQHAEYCFQHYAST-------ETCFSAFK 544
C S+L YF+ +D + DD+ H YC + AS + C F
Sbjct: 502 CTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFG 561
Query: 545 APLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENGKAVAWEKAFVKLVKEELLP 604
P+ P LGG+ NY+ A+A V+T+PVNN + + +A AWEK F+ VK P
Sbjct: 562 GPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDT-EKLQRAQAWEKEFINFVKNYKNP 621
Query: 605 LVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFVYISVALGDSSISSSFYLSS 664
NLT+SF++E SIE+EL RES +DV T+V+SY +MF+YIS+ALG + S
Sbjct: 622 -----NLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLVDS 681
Query: 665 KVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQ 724
KV LG++G+++V+ SV S+G FS IG+ TLI++EVIPFLVLAVGVDN+ ILV A +R
Sbjct: 682 KVSLGIAGILIVLSSVACSLGVFSYIGLPLTLIVIEVIPFLVLAVGVDNIFILVQAYQRD 741
Query: 725 P--FELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMPACRVFSMFAALAVLLDF 784
TL++++ L EV PS+ L+S SE +AF +GA MPA FS+FA LAV +DF
Sbjct: 742 ERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGALSVMPAVHTFSLFAGLAVFIDF 801
Query: 785 ILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFNQGKPGLLSWYMKDVHAP 844
+LQ++ FV+L+ DI R E +R+D F C++ Q L + K+ ++P
Sbjct: 802 LLQITCFVSLLGLDIKRQEKNRLDIFCCVRGAEDGTSV-----QASESCLFRFFKNSYSP 861
Query: 845 LLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDSYLQDYFDDLAEYLRIGP 904
LL ++ VI IFVG IA+ K+++GL+Q + +P DSY+ DYF +++YL GP
Sbjct: 862 LLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGLDQSLSMPDDSYMVDYFKSISQYLHAGP 921
Query: 905 PLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLTPELNYIAKPAASWLDDFL 964
P+YFV+++ + + S+ N +C C+++SL+ +I A+ I +SW+DD+
Sbjct: 922 PVYFVLEEGHDYTSSKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAPSSWIDDYF 981
Query: 965 VWLSPDAFGCCR------KFTNGSYCPPDDQPPCCFPDEGSCDSSGGVCKDCTTCFHYSD 1024
W+ P + CCR +F N S P C C +
Sbjct: 982 DWVKPQS-SCCRVDNITDQFCNASVVDP----------------------ACVRCRPLTP 1041
Query: 1025 LVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGIIKASEFRTYHTP 1084
RP F LP FL+ P+ C KGGH AY+++VN+ + A+ F TYHT
Sbjct: 1042 EGKQRPQGGDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILLGHGTRVGATYFMTYHTV 1101
Query: 1085 LNKQGDYVNALRAAKEFSSKMSDSLKMD-----VFPYSVFYIFFEQYLDIWKIALINIAV 1144
L D+++AL+ A+ +S +++++ ++ VFPYSVFY+F+EQYL I + N+ V
Sbjct: 1102 LQTSADFIDALKKARLIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGV 1161
Query: 1145 ALG------------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLMSIGIA 1168
+LG LWS+ I+ +AM+++++ G+M + I LNAVS+VNL+MS GI+
Sbjct: 1162 SLGAIFLVTMVLLGCELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGIS 1198
BLAST of MS004994 vs. ExPASy Swiss-Prot
Match:
Q6T3U3 (NPC1-like intracellular cholesterol transporter 1 OS=Rattus norvegicus OX=10116 GN=Npc1l1 PE=1 SV=1)
HSP 1 Score: 521.2 bits (1341), Expect = 3.1e-146
Identity = 411/1258 (32.67%), Postives = 627/1258 (49.84%), Query Frame = 0
Query: 2 RHSTEYCAMYDICGARGD--GKV-----LNCPYGSPSVKPDELFSEKIQSLCPTISGN-- 61
+H C Y+ CG + G + ++C +P+ +Q +CP +
Sbjct: 26 KHEAGVCTFYEECGKNPELSGGLTSLSNVSCLSNTPARHVTGEHLALLQRICPRLYNGPN 85
Query: 62 ---VCCTEAQFDTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEV 121
CC+ Q +L S + L CPAC NF++L C +CSP QSLFINVT + E
Sbjct: 86 TTFACCSTKQLLSLESSMSITKALLTRCPACSDNFVSLHCHNTCSPDQSLFINVTRVVER 145
Query: 122 GGN--MTVDGIDYYVTEKFGKGLYDSCKDVKFGTMNTRAI-DFVGGGAKSFEELFAFLGK 181
G V + + F + Y+SC V+ + A+ G + +L
Sbjct: 146 GAGEPPAVVAYEAFYQRSFAEKAYESCSQVRIPAAASLAVGSMCGVYGSALCNAQRWLNF 205
Query: 182 KAAPGFPGSPYTINFKLNTPKSS---PMELMNVSVYSC----GDTSLGCSCGDCPLSPVC 241
+ G +P I F L P + ++ +N + C GD S CSC DC S C
Sbjct: 206 QGDTGNGLAPLDITFHLLEPGQALPDGIQPLNGKIAPCNESQGDDSAVCSCQDCAAS--C 265
Query: 242 SSLEPPSPPKSNACSIRI--WFLEVGCIDFSITIIYIIFISAFLGWALFHPARERRFSAR 301
+ PP + + R+ W + T ++++ +SA L R R S R
Sbjct: 266 PVIPPPEALRPSFYMGRMPGWL----ALIIIFTAVFVL-LSAVL-------VRLRVVSNR 325
Query: 302 EEPLLNIGDGHEISSVNLEENEYSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVA 361
+ N E A K L ++ + TI G F++N+G VA
Sbjct: 326 NK--------------NKAEGPQEAPK-----LPHKHKLSPHTILG---RFFQNWGTRVA 385
Query: 362 RNPILVLFSSLSIVLILCVGLVRFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQ 421
P+ VL S +V+ L GL ++ T P +LW S+A EK F D PF+R Q
Sbjct: 386 SWPLTVLALSFIVVIALAAGLTFIELTTDPVELWSAPKSQARKEKSFHDEHFGPFFRTNQ 445
Query: 422 MIIATKPEERH---------GRAPRIVTEDNILLLFDIQDKVNELV--ANYSGSLVSLND 481
+ + + + I++ D +L L ++Q+++ L + + +SL D
Sbjct: 446 IFVTARNRSSYKYDSLLLGSKNFSGILSLDFLLELLELQERLRHLQVWSPEAERNISLQD 505
Query: 482 ICLKPLG------EDCATQSILQYFK------MDPENYDDYGGV------QHAEYC---- 541
IC PL DC S+LQYF+ M N G H YC
Sbjct: 506 ICYAPLNPYNTSLSDCCVNSLLQYFQNNRTLLMLTANQTLNGQTSLVDWKDHFLYCANAP 565
Query: 542 --FQHYASTE-TCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENGK 601
F+ S +C + + AP+ P ++GG+ G +YSEA A ++T+ +NN A +
Sbjct: 566 LTFKDGTSLALSCMADYGAPVFPFLAVGGYQGTDYSEAEALIITFSLNN-YPADDPRMAQ 625
Query: 602 AVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFVY 661
A WE+AF+K + E S ++FS+E S+E+E+ R + D+ VSY+++F+Y
Sbjct: 626 AKLWEEAFLK--EMESFQRNTSDKFQVAFSAERSLEDEINRTTIQDLPVFAVSYIIVFLY 685
Query: 662 ISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFLV 721
IS+ALG S S + SK LGL GVI+V+ +VL ++GF+S +GV S+L+I++V+PFLV
Sbjct: 686 ISLALGSYSRCSRVAVESKATLGLGGVIVVLGAVLAAMGFYSYLGVPSSLVIIQVVPFLV 745
Query: 722 LAVGVDNMCILVHAVKRQPFELTLEER---ISSALVEVGPSITLASLSEILAFAVGAFVP 781
LAVG DN+ I V +R P + E+R I L V PS+ L SLSE + F +GA P
Sbjct: 746 LAVGADNIFIFVLEYQRLP-RMPGEQREAHIGRTLGSVAPSMLLCSLSEAICFFLGALTP 805
Query: 782 MPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVD---CFPCIKVTPHSDEP 841
MPA R F++ + LA++LDF+LQ++AFVAL+ D R E R D CF K+ P +
Sbjct: 806 MPAVRTFALTSGLAIILDFLLQMTAFVALLSLDSKRQEASRPDVLCCFSTRKLPPPKE-- 865
Query: 842 NQGFNQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIV 901
K GLL + + ++AP L ++ V+++F+ + L I VGL+Q++
Sbjct: 866 -------KEGLLLRFFRKIYAPFLLHRFIRPVVMLLFLTLFGANLYLMCNINVGLDQELA 925
Query: 902 LPRDSYLQDYFDDLAEYLRIGPPLYFV-VKDYNYSSESRQTNQLCSISQCDSNSLLNEIS 961
LP+DSYL DYF L YL +GPP+YFV +N+SSE+ N CS + C S SL +I
Sbjct: 926 LPKDSYLIDYFLFLNRYLEVGPPVYFVTTSGFNFSSEAGM-NATCSSAGCKSFSLTQKIQ 985
Query: 962 RASLTPELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNG----SYCPPDDQPPCCFPDE 1021
AS P+ +Y+A A+SW+DDF+ WL+P + CCR + G +CP
Sbjct: 986 YASEFPDQSYVAIAASSWVDDFIDWLTPSS-SCCRLYIRGPHKDEFCP------------ 1045
Query: 1022 GSCDSSGGVCKDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSV 1081
S D+S K+C L RPT QF + LPWFLN P+ C KGG AY SV
Sbjct: 1046 -STDTSFNCLKNCMN----RTLGPVRPTAEQFHKYLPWFLNDPPNIRCPKGGLAAYRTSV 1105
Query: 1082 NLNGYESGIIKASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLK--------MDVFP 1141
NL+ G + AS+F YH PL D+ ALRA++ ++ ++ L+ +VFP
Sbjct: 1106 NLS--SDGQVIASQFMAYHKPLRNSQDFTEALRASRLLAANITADLRKVPGTDPNFEVFP 1165
Query: 1142 YSVFYIFFEQYLDIWKIALINIAVA-----------LGYLWSSGII-ILVLAMIIIDLMG 1168
Y++ +F++QYL + + +A+ LG SGI+ +L + MI++D +G
Sbjct: 1166 YTISNVFYQQYLTVLPEGIFTLALCFVPTFVVCYLLLGLDMCSGILNLLSIIMILVDTIG 1213
BLAST of MS004994 vs. ExPASy Swiss-Prot
Match:
Q9UHC9 (NPC1-like intracellular cholesterol transporter 1 OS=Homo sapiens OX=9606 GN=NPC1L1 PE=1 SV=2)
HSP 1 Score: 516.5 bits (1329), Expect = 7.6e-145
Identity = 414/1286 (32.19%), Postives = 635/1286 (49.38%), Query Frame = 0
Query: 3 HSTEYCAMYDICGARGD--GKVL-----NCPYGSPSVKPDELFSEKIQSLCPTI----SG 62
H YCA YD CG + G ++ +C +P+ K +Q +CP + +
Sbjct: 28 HQPGYCAFYDECGKNPELSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNT 87
Query: 63 NVCCTEAQFDTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGG 122
CC+ Q +L + + L CPAC NF+NL C +CSP QSLFINVT ++++G
Sbjct: 88 QACCSAKQLVSLEASLSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGA 147
Query: 123 NM--TVDGIDYYVTEKFGKGLYDSCKDVKFGTMNTRAI-DFVGGGAKSFEELFAFLGKKA 182
V + + F + YDSC V+ T A+ G + +L +
Sbjct: 148 GQLPAVVAYEAFYQHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQG 207
Query: 183 APGFPGSPYTINFKLNTP---KSSPMELMNVSVYSC----GDTSLGCSCGDCPLSPVCSS 242
G +P I F L P S ++ +N V C GD CSC DC S C +
Sbjct: 208 DTGNGLAPLDITFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAAS--CPA 267
Query: 243 LEPPSPPKSNACSIRIWFLEVGCIDFSITIIYII-----FISAFLGWALFHPARERRFSA 302
+ P S +G + S+ +I I+ ++ L PAR++
Sbjct: 268 IARPQALDST--------FYLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKS--- 327
Query: 303 REEPLLNIGDGHEISSVNLEENEYSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWV 362
V+ ++ + K+ TL + F++ +G WV
Sbjct: 328 --------------KMVDPKKGTSLSDKLSFSTHTL------------LGQFFQGWGTWV 387
Query: 363 ARNPILVLFSSLSIVLILCVGLVRFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIE 422
A P+ +L S+ V+ L GLV ++ T P +LW S+A +EK F D PF+R
Sbjct: 388 ASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARSEKAFHDQHFGPFFRTN 447
Query: 423 QMIIATKPEERHGR------APR----IVTEDNILLLFDIQDKVNELV--ANYSGSLVSL 482
Q+I+ T P R P+ I+ D +L L ++Q+++ L + + +SL
Sbjct: 448 QVIL-TAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQERLRHLQVWSPEAQRNISL 507
Query: 483 NDICLKPLGE------DCATQSILQYFK--------------MDPENYDDYGGVQHAEYC 542
DIC PL DC S+LQYF+ M + D+ H YC
Sbjct: 508 QDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQTLMGQTSQVDW--KDHFLYC 567
Query: 543 F-------QHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGN 602
A +C + + AP+ P ++GG+ G +YSEA A ++T+ +NN A
Sbjct: 568 ANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSEAEALIMTFSLNN-YPAGDP 627
Query: 603 ENGKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLV 662
+A WE+AF++ ++ + +T F +E S+E+E+ R + D+ SY+V
Sbjct: 628 RLAQAKLWEEAFLEEMRAFQRRMAGMFQVT--FMAERSLEDEINRTTAEDLPIFATSYIV 687
Query: 663 MFVYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVI 722
+F+YIS+ALG S S + SK LGL GV +V+ +V+ ++GFFS +G++S+L+I++V+
Sbjct: 688 IFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMAAMGFFSYLGIRSSLVILQVV 747
Query: 723 PFLVLAVGVDNMCILV---HAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVG 782
PFLVL+VG DN+ I V + R+P E E I AL V PS+ L SLSE + F +G
Sbjct: 748 PFLVLSVGADNIFIFVLEYQRLPRRPGE-PREVHIGRALGRVAPSMLLCSLSEAICFFLG 807
Query: 783 AFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDE 842
A PMPA R F++ + LAV+LDF+LQ+SAFVAL+ D R E R+D C+K
Sbjct: 808 ALTPMPAVRTFALTSGLAVILDFLLQMSAFVALLSLDSKRQEASRLDVCCCVK-PQELPP 867
Query: 843 PNQGFNQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALST--KIEVGLEQ 902
P QG GLL + + +AP L W + V+++F+ L G++L + I VGL+Q
Sbjct: 868 PGQG-----EGLLLGFFQKAYAPFLLHWITRGVVLLLFL--ALFGVSLYSMCHISVGLDQ 927
Query: 903 KIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVK-DYNYSSESRQTNQLCSISQCDSNSLLN 962
++ LP+DSYL DYF L Y +G P+YFV YN+SSE+ N +CS + C++ S
Sbjct: 928 ELALPKDSYLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEAGM-NAICSSAGCNNFSFTQ 987
Query: 963 EISRASLTPELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNG----SYCPPDDQPPCCF 1022
+I A+ PE +Y+A PA+SW+DDF+ WL+P + CCR + +G +CP
Sbjct: 988 KIQYATEFPEQSYLAIPASSWVDDFIDWLTPSS--CCRLYISGPNKDKFCP--------- 1047
Query: 1023 PDEGSCDSSGGVCKDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYT 1082
S +S K+C + + + RP+ QF + LPWFLN P+ C KGG AY+
Sbjct: 1048 ----STVNSLNCLKNCMSI----TMGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLAAYS 1107
Query: 1083 NSVNLNG---------------YESGIIKA----------SEFRTYHTPLNKQGDYVNAL 1142
SVNL SG I A S F YH PL DY AL
Sbjct: 1108 TSVNLTSDGQVLDTVAILSPRLEYSGTISAHCNLYLLDSTSRFMAYHKPLKNSQDYTEAL 1167
Query: 1143 RAAKEFSSKMSDSLK--------MDVFPYSVFYIFFEQYLDIWKIALINIAVA------- 1168
RAA+E ++ ++ L+ +VFPY++ +F+EQYL I L +++
Sbjct: 1168 RAARELAANITADLRKVPGTDPAFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAV 1227
BLAST of MS004994 vs. ExPASy TrEMBL
Match:
A0A6J1CI77 (Niemann-Pick C1 protein OS=Momordica charantia OX=3673 GN=LOC111011166 PE=3 SV=1)
HSP 1 Score: 2273.0 bits (5889), Expect = 0.0e+00
Identity = 1158/1178 (98.30%), Postives = 1159/1178 (98.39%), Query Frame = 0
Query: 1 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 60
ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF
Sbjct: 49 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 108
Query: 61 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 120
DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY
Sbjct: 109 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 168
Query: 121 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 180
YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN
Sbjct: 169 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 228
Query: 181 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 240
FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL
Sbjct: 229 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 288
Query: 241 EVGCIDFSITIIYIIFISAFLGWALFHPARERRFSAREEPLLNIGDGHEISSVNLEENEY 300
E CIDFSITIIYIIFISAFLGWALFHPARERRFSAREEPLLNIGDGHEISSVNLEENEY
Sbjct: 289 ESSCIDFSITIIYIIFISAFLGWALFHPARERRFSAREEPLLNIGDGHEISSVNLEENEY 348
Query: 301 SATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLVR 360
SATK LTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLVR
Sbjct: 349 SATKEHGAQLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLVR 408
Query: 361 FKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDNI 420
FKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDNI
Sbjct: 409 FKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDNI 468
Query: 421 LLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQH 480
LLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQH
Sbjct: 469 LLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQH 528
Query: 481 AEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENG 540
AEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENG
Sbjct: 529 AEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENG 588
Query: 541 KAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFV 600
KAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFV
Sbjct: 589 KAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFV 648
Query: 601 YISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFL 660
YISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFL
Sbjct: 649 YISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFL 708
Query: 661 VLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMP 720
VLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMP
Sbjct: 709 VLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMP 768
Query: 721 ACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFN 780
ACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFN
Sbjct: 769 ACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFN 828
Query: 781 QGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDS 840
QGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDS
Sbjct: 829 QGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDS 888
Query: 841 YLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLTP 900
YLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLTP
Sbjct: 889 YLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLTP 948
Query: 901 ELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGVC 960
ELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGVC
Sbjct: 949 ELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGVC 1008
Query: 961 KDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGII 1020
KDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGII
Sbjct: 1009 KDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGII 1068
Query: 1021 KASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLDIWKIA 1080
KASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMD+FPYSVFYIFFEQYLDIWKIA
Sbjct: 1069 KASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDIFPYSVFYIFFEQYLDIWKIA 1128
Query: 1081 LINIAVALG-----------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLM 1140
LINIAVALG LWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLM
Sbjct: 1129 LINIAVALGAVFVVSLVITSCLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLM 1188
Query: 1141 SIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1168
SIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV
Sbjct: 1189 SIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1226
BLAST of MS004994 vs. ExPASy TrEMBL
Match:
A0A6J1I473 (Niemann-Pick C1 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111469740 PE=3 SV=1)
HSP 1 Score: 2082.0 bits (5393), Expect = 0.0e+00
Identity = 1053/1179 (89.31%), Postives = 1106/1179 (93.81%), Query Frame = 0
Query: 1 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 60
ERHS EYCAMYDICGAR DGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF
Sbjct: 49 ERHSEEYCAMYDICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 108
Query: 61 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 120
+TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSI+EVGG MTVDGIDY
Sbjct: 109 ETLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSIAEVGGKMTVDGIDY 168
Query: 121 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 180
+VTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEE FAFLG+K PG PGSPY+IN
Sbjct: 169 FVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEEFFAFLGQKVGPGIPGSPYSIN 228
Query: 181 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 240
FK+NT K S +ELMNVSVYSCGDTSLGCSCGDCP SP CSSLEPPSPPKSNAC+IRIW L
Sbjct: 229 FKVNTDKPSQVELMNVSVYSCGDTSLGCSCGDCPSSPACSSLEPPSPPKSNACTIRIWSL 288
Query: 241 EVGCIDFSITIIYIIFISAFLGWALFHPARER-RFSAREEPLLNIGDGHEISSVNLEENE 300
+ CIDFSITI+Y+IF+SAFLGWALFHP RER RFSAREEPLLNIGD E++SVNLEENE
Sbjct: 289 KSSCIDFSITILYVIFVSAFLGWALFHPTRERSRFSAREEPLLNIGDDGEVNSVNLEENE 348
Query: 301 YSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLV 360
ATK LTLRNG+QLSTIQGYI++FYRNYGAWVARNPILVL +SLSIVLILCVGLV
Sbjct: 349 NGATKEHGVHLTLRNGVQLSTIQGYIASFYRNYGAWVARNPILVLCTSLSIVLILCVGLV 408
Query: 361 RFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDN 420
RFKVET+PEKLWVGHGSKAAAEKQFFDS LAPFYRIEQ+IIATKP R APRIVTEDN
Sbjct: 409 RFKVETRPEKLWVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPSGRQSTAPRIVTEDN 468
Query: 421 ILLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQ 480
ILLLFDIQ+KVNELVANYSGS+VSL DICLKPLGEDCATQSILQYFKMDPEN+D+YGGV+
Sbjct: 469 ILLLFDIQNKVNELVANYSGSVVSLTDICLKPLGEDCATQSILQYFKMDPENFDEYGGVE 528
Query: 481 HAEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNEN 540
H EYC QHY STETCFSAFKAPLDPSTSLGGF GNNYSEASAFVVTYPVNNAID VG+EN
Sbjct: 529 HVEYCLQHYTSTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDDVGDEN 588
Query: 541 GKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMF 600
GKA+AWEKAFVKLVKEELLPLVQS+NLTLSFSSESSIEEELKRESTADVLTI VSYLVMF
Sbjct: 589 GKAIAWEKAFVKLVKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIAVSYLVMF 648
Query: 601 VYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPF 660
YISVALGDS+ISSSFYLSSKVLLGLSGV+LVVLSVLGSVGFFSA+GVKSTLIIMEVIPF
Sbjct: 649 AYISVALGDSNISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALGVKSTLIIMEVIPF 708
Query: 661 LVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPM 720
LVLAVGVDNMCILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVG FVPM
Sbjct: 709 LVLAVGVDNMCILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPM 768
Query: 721 PACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGF 780
PACRVFSMFAALAV LDFILQLSAFVALIVFDILRAE+HRVDCFPCIKV P SDEPNQGF
Sbjct: 769 PACRVFSMFAALAVFLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPPRSDEPNQGF 828
Query: 781 NQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRD 840
NQ + GLLS YMKDVHAPLLGLWGVKIAV++IFVG TLG I LSTKIEVGLEQKIVLPRD
Sbjct: 829 NQRRLGLLSRYMKDVHAPLLGLWGVKIAVVIIFVGLTLGSITLSTKIEVGLEQKIVLPRD 888
Query: 841 SYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLT 900
SYLQ YFDDLAEYLRIGPPLYFVVKDYNYSS+SR TNQLCSISQCDSNSLLNEISRASLT
Sbjct: 889 SYLQGYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLT 948
Query: 901 PELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGV 960
PELNYIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSYCPPDDQPPCC PDEG CD+S GV
Sbjct: 949 PELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCIPDEGFCDASEGV 1008
Query: 961 CKDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1020
C+DCTTCFH+SDLVAGRPTT+QF+EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI
Sbjct: 1009 CQDCTTCFHHSDLVAGRPTTVQFREKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1068
Query: 1021 IKASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLDIWKI 1080
IKASEFR+YHTPLNKQGDYVNALRAAKEF SK+SDSLKMD+FPYSVFYIFFEQYLDIWK
Sbjct: 1069 IKASEFRSYHTPLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKT 1128
Query: 1081 ALINIAVALG-----------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLL 1140
AL+NIA+ALG LWSSGIIILVLAMIIIDLMG+MAILNIQLNAVSVVN+L
Sbjct: 1129 ALMNIAIALGAIFIVSLVITSCLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNIL 1188
Query: 1141 MSIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1168
MSIGIAVEFCVH+VHAF VS GDRSQR++EAL TMGASV
Sbjct: 1189 MSIGIAVEFCVHLVHAFSVSCGDRSQRSQEALTTMGASV 1227
BLAST of MS004994 vs. ExPASy TrEMBL
Match:
A0A6J1G6Q4 (Niemann-Pick C1 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111451262 PE=3 SV=1)
HSP 1 Score: 2080.4 bits (5389), Expect = 0.0e+00
Identity = 1055/1179 (89.48%), Postives = 1103/1179 (93.55%), Query Frame = 0
Query: 1 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 60
ERHS EYCAMYDICGAR DGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF
Sbjct: 49 ERHSEEYCAMYDICGARSDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 108
Query: 61 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 120
+TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSI+EVGG MTVDGIDY
Sbjct: 109 ETLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSIAEVGGKMTVDGIDY 168
Query: 121 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 180
+VTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKS EE FAFLG+K PG PGSPY+IN
Sbjct: 169 FVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSSEEFFAFLGQKVGPGIPGSPYSIN 228
Query: 181 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 240
FK NT K S MELMNVSVYSCGDTSLGCSCGDCP SP CSSLEPPSPPKSNAC+IRIW L
Sbjct: 229 FKANTDKPSQMELMNVSVYSCGDTSLGCSCGDCPSSPACSSLEPPSPPKSNACTIRIWSL 288
Query: 241 EVGCIDFSITIIYIIFISAFLGWALFHPARE-RRFSAREEPLLNIGDGHEISSVNLEENE 300
+ CIDFSITI+Y+IFISAFLGWALFHP RE RRFSAREEPLLNIGD E++SVNLEENE
Sbjct: 289 KSSCIDFSITILYVIFISAFLGWALFHPTRERRRFSAREEPLLNIGDDGEVNSVNLEENE 348
Query: 301 YSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLV 360
ATK LTLRNG+QLSTIQGYI++FYRNYGAWVARNPILVL +SLSIVLILCVGLV
Sbjct: 349 NGATKEHGVHLTLRNGVQLSTIQGYIASFYRNYGAWVARNPILVLCTSLSIVLILCVGLV 408
Query: 361 RFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDN 420
RFKVET+PEKLWVGHGSKAAAEKQFFDS LAPFYRIEQ+IIATKP R APRIVTEDN
Sbjct: 409 RFKVETRPEKLWVGHGSKAAAEKQFFDSHLAPFYRIEQLIIATKPSGRQSTAPRIVTEDN 468
Query: 421 ILLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQ 480
ILLLFDIQ+KVNELVANYSGS+VSL DICLKPLGEDCATQSILQYFKMDPEN+D+YGGV+
Sbjct: 469 ILLLFDIQNKVNELVANYSGSVVSLTDICLKPLGEDCATQSILQYFKMDPENFDEYGGVE 528
Query: 481 HAEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNEN 540
H EYC QHY STETCFSAFKAPLDPSTSLGGF GNNYSEASAFVVTYPVNNAID VG+EN
Sbjct: 529 HVEYCLQHYTSTETCFSAFKAPLDPSTSLGGFVGNNYSEASAFVVTYPVNNAIDDVGDEN 588
Query: 541 GKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMF 600
GKA+AWEKAFVKL KEELLPLVQS+NLTLSFSSESSIEEELKRESTADVLTI VSYLVMF
Sbjct: 589 GKAIAWEKAFVKLAKEELLPLVQSKNLTLSFSSESSIEEELKRESTADVLTIAVSYLVMF 648
Query: 601 VYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPF 660
YISVALGDS+ISSSFYLSSKVLLGLSGV+LVVLSVLGSVGFFSA+GVKSTLIIMEVIPF
Sbjct: 649 AYISVALGDSNISSSFYLSSKVLLGLSGVLLVVLSVLGSVGFFSALGVKSTLIIMEVIPF 708
Query: 661 LVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPM 720
LVLAVGVDNMCILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVG FVPM
Sbjct: 709 LVLAVGVDNMCILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPM 768
Query: 721 PACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGF 780
PACRVFSMFAALAV LDFILQLSAFVALIVFDI RAE+HRVDCFPCIKV P SDEPNQGF
Sbjct: 769 PACRVFSMFAALAVFLDFILQLSAFVALIVFDIRRAENHRVDCFPCIKVPPRSDEPNQGF 828
Query: 781 NQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRD 840
NQ + GLLS YMKDVHAPLLGLWGVKIAV++IFVG TLG IALSTKIEVGLEQKIVLPRD
Sbjct: 829 NQRRLGLLSRYMKDVHAPLLGLWGVKIAVVIIFVGLTLGSIALSTKIEVGLEQKIVLPRD 888
Query: 841 SYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLT 900
SYLQ YFDDLAEYLRIGPPLYFVVKDYNYSS+SR TNQLCSISQCDSNSLLNEISRASLT
Sbjct: 889 SYLQGYFDDLAEYLRIGPPLYFVVKDYNYSSKSRHTNQLCSISQCDSNSLLNEISRASLT 948
Query: 901 PELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGV 960
PELNYIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSYCPPDDQPPCC PDEG CD+S GV
Sbjct: 949 PELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCIPDEGFCDASEGV 1008
Query: 961 CKDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1020
C+DCTTCFH+SDLVAGRPTT+QF+EKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI
Sbjct: 1009 CQDCTTCFHHSDLVAGRPTTVQFREKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1068
Query: 1021 IKASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLDIWKI 1080
IKASEFR+YHTPLNKQGDYVNALRAAKEF SK+SDSLKMD+FPYSVFYIFFEQYLDIWK
Sbjct: 1069 IKASEFRSYHTPLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWKT 1128
Query: 1081 ALINIAVALG-----------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLL 1140
AL+NIA+ALG LWSSGIIILVLAMIIIDLMG+MAILNIQLNAVSVVN+L
Sbjct: 1129 ALMNIAIALGAIFIVSLVITSCLWSSGIIILVLAMIIIDLMGVMAILNIQLNAVSVVNIL 1188
Query: 1141 MSIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1168
MSIGIAVEFCVH+VHAF VS GDRSQRAREAL TMGASV
Sbjct: 1189 MSIGIAVEFCVHLVHAFSVSCGDRSQRAREALTTMGASV 1227
BLAST of MS004994 vs. ExPASy TrEMBL
Match:
A0A1S3BJR3 (Niemann-Pick C1 protein-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490460 PE=3 SV=1)
HSP 1 Score: 2060.0 bits (5336), Expect = 0.0e+00
Identity = 1038/1179 (88.04%), Postives = 1100/1179 (93.30%), Query Frame = 0
Query: 1 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 60
ERH+ EYCAMYDICG R DGKVLNCPYGSPSVKPDELFS KIQSLCPTISGNVCCTEAQF
Sbjct: 49 ERHAAEYCAMYDICGTRSDGKVLNCPYGSPSVKPDELFSAKIQSLCPTISGNVCCTEAQF 108
Query: 61 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 120
+TLRSQVQQAIPLFVGCPAC+RNFLNLFCELSCSPRQSLFINVTSI+EVGG+MTVDGIDY
Sbjct: 109 ETLRSQVQQAIPLFVGCPACMRNFLNLFCELSCSPRQSLFINVTSIAEVGGSMTVDGIDY 168
Query: 121 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 180
YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLG+K APGFPGSPY+IN
Sbjct: 169 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVAPGFPGSPYSIN 228
Query: 181 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 240
FK+N KSS MELMNVSVYSCGDTSLGCSCGDCP SPVCSSLEPPSPPKSNAC+I+IW L
Sbjct: 229 FKVNPSKSSQMELMNVSVYSCGDTSLGCSCGDCPSSPVCSSLEPPSPPKSNACTIKIWSL 288
Query: 241 EVGCIDFSITIIYIIFISAFLGWALFHPARERR-FSAREEPLLNIGDGHEISSVNLEENE 300
++ CIDFSITI+Y+IFIS+FLGWALFHP +E R FS+REEPLLNIGD EI SVNL ENE
Sbjct: 289 KISCIDFSITILYVIFISSFLGWALFHPTKENRGFSSREEPLLNIGDDGEIKSVNLTENE 348
Query: 301 YSATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLV 360
T+ LT+RNG+QLSTIQ YISNFYR+YGAWVARNPILVL SLSIVLILCVGLV
Sbjct: 349 NVTTEEHGVHLTVRNGVQLSTIQRYISNFYRDYGAWVARNPILVLCMSLSIVLILCVGLV 408
Query: 361 RFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDN 420
FKVET+PEKLWVGHGS+AAAEKQFFDS LAPFYRIEQ+IIATKP +H RAPRIVTEDN
Sbjct: 409 CFKVETRPEKLWVGHGSRAAAEKQFFDSNLAPFYRIEQLIIATKPGGKHDRAPRIVTEDN 468
Query: 421 ILLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQ 480
ILLLFDIQ+KVNELVANYSGS+VSLNDICLKPLGEDCATQSILQYFKM+PEN+DDYGGV+
Sbjct: 469 ILLLFDIQNKVNELVANYSGSVVSLNDICLKPLGEDCATQSILQYFKMNPENFDDYGGVE 528
Query: 481 HAEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNEN 540
HAEYCFQHY S+ETCFSAFKAPLDPSTSLGGF G+NYSEASAFV+TYPVNNAIDAVGNEN
Sbjct: 529 HAEYCFQHYTSSETCFSAFKAPLDPSTSLGGFFGSNYSEASAFVITYPVNNAIDAVGNEN 588
Query: 541 GKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMF 600
GKA+AWEKAFVKL KEEL+PLV SRNLTLSFSSESSIEEELKRESTAD+LTI VSYLVMF
Sbjct: 589 GKAIAWEKAFVKLAKEELMPLVHSRNLTLSFSSESSIEEELKRESTADILTIAVSYLVMF 648
Query: 601 VYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPF 660
YISVALGDS+ISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIG+KSTLIIMEVIPF
Sbjct: 649 AYISVALGDSNISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGIKSTLIIMEVIPF 708
Query: 661 LVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPM 720
LVLAVGVDNMCILVHAVKRQP+EL+LE+RISSALVEVGPSITLASLSEILAFAVG FVPM
Sbjct: 709 LVLAVGVDNMCILVHAVKRQPYELSLEDRISSALVEVGPSITLASLSEILAFAVGTFVPM 768
Query: 721 PACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGF 780
PACRVFS+FAALAVLLDFILQLSAFVALIV DILRAEDHRVDCFPCIKV PHSDEPNQGF
Sbjct: 769 PACRVFSLFAALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCIKVHPHSDEPNQGF 828
Query: 781 NQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRD 840
NQG+ GLLS YMKDVHAPLLG WGVKI V+VIFVG TLG IALSTKIEVGLEQKIVLPRD
Sbjct: 829 NQGRHGLLSLYMKDVHAPLLGFWGVKIVVVVIFVGLTLGSIALSTKIEVGLEQKIVLPRD 888
Query: 841 SYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLT 900
SYLQDYFDDLAEYLRIGPPLYFVVKDYNYSS SRQTNQLCSIS CDSNSLLNEISRASLT
Sbjct: 889 SYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSRSRQTNQLCSISHCDSNSLLNEISRASLT 948
Query: 901 PELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGV 960
PELNYIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSYCPPDDQPPCCFPDEG CDSS GV
Sbjct: 949 PELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCFPDEGFCDSSEGV 1008
Query: 961 CKDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGI 1020
C+DCTTCF +SDLV RPTT QF+EKLPWFLNSLPSADCAKGGHGAYTNSVNL GYE GI
Sbjct: 1009 CRDCTTCFRHSDLVGDRPTTEQFKEKLPWFLNSLPSADCAKGGHGAYTNSVNLKGYEIGI 1068
Query: 1021 IKASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLDIWKI 1080
IKASEFR+YHTPLNKQGDYVNALRAAKEF SK+SDSLKMD+FPYSVFYIFFEQYLDIW
Sbjct: 1069 IKASEFRSYHTPLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLDIWNT 1128
Query: 1081 ALINIAVALG-----------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLL 1140
AL+NIA+ALG LW SG+IILVLAMI+IDL+G+MAIL IQLNAVSVVN+L
Sbjct: 1129 ALMNIAIALGAIFIVSLVITSSLWCSGMIILVLAMIVIDLLGVMAILKIQLNAVSVVNIL 1188
Query: 1141 MSIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1168
MSIGIAVEFCVH+VHAF VS GDR+QRA+EAL T+GASV
Sbjct: 1189 MSIGIAVEFCVHLVHAFSVSCGDRNQRAQEALSTIGASV 1227
BLAST of MS004994 vs. ExPASy TrEMBL
Match:
A0A5A7U0V7 (Niemann-Pick C1 protein-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G001120 PE=3 SV=1)
HSP 1 Score: 2059.6 bits (5335), Expect = 0.0e+00
Identity = 1039/1183 (87.83%), Postives = 1101/1183 (93.07%), Query Frame = 0
Query: 1 ERHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQF 60
ERH+ EYCAMYDICG R DGKVLNCPYGSPSVKPDELFS KIQSLCPTISGNVCCTEAQF
Sbjct: 26 ERHAAEYCAMYDICGTRSDGKVLNCPYGSPSVKPDELFSAKIQSLCPTISGNVCCTEAQF 85
Query: 61 DTLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDY 120
+TLRSQVQQAIPLFVGCPAC+RNFLNLFCELSCSPRQSLFINVTSI+EVGG+MTVDGIDY
Sbjct: 86 ETLRSQVQQAIPLFVGCPACMRNFLNLFCELSCSPRQSLFINVTSIAEVGGSMTVDGIDY 145
Query: 121 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTIN 180
YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLG+K APGFPGSPY+IN
Sbjct: 146 YVTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGQKVAPGFPGSPYSIN 205
Query: 181 FKLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFL 240
FK+N KSS MELMNVSVYSCGDTSLGCSCGDCP SPVCSSLEPPSPPKSNAC+I+IW L
Sbjct: 206 FKVNPSKSSQMELMNVSVYSCGDTSLGCSCGDCPSSPVCSSLEPPSPPKSNACTIKIWSL 265
Query: 241 EVGCIDFSITIIYIIFISAFLGWALFHPARERR-FSAREEPLLNIGDGHEISSVNLEENE 300
++ CIDFSITI+Y+IFIS+FLGWALFHP +E R FS+REEPLLNIGD EI SVNL ENE
Sbjct: 266 KISCIDFSITILYVIFISSFLGWALFHPTKENRGFSSREEPLLNIGDDGEIKSVNLAENE 325
Query: 301 YSATKVGLDV----LTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILC 360
T+ G LT+RNG+QLSTIQ YISNFYR+YGAWVARNPILVL SLSIVLILC
Sbjct: 326 NVTTEAGFAEHGVHLTVRNGVQLSTIQRYISNFYRDYGAWVARNPILVLCMSLSIVLILC 385
Query: 361 VGLVRFKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIV 420
VGLV FKVET+PEKLWVGHGS+AAAEKQFFDS LAPFYRIEQ+IIATKP +H RAPRIV
Sbjct: 386 VGLVCFKVETRPEKLWVGHGSRAAAEKQFFDSNLAPFYRIEQLIIATKPGGKHDRAPRIV 445
Query: 421 TEDNILLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDY 480
TEDNILLLFDIQ+KVNELVANYSGS+VSLNDICLKPLGEDCATQSILQYFKM+PEN+DDY
Sbjct: 446 TEDNILLLFDIQNKVNELVANYSGSVVSLNDICLKPLGEDCATQSILQYFKMNPENFDDY 505
Query: 481 GGVQHAEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAV 540
GGV+HAEYCFQHY S+ETCFSAFKAPLDPSTSLGGF G+NYSEASAFV+TYPVNNAIDAV
Sbjct: 506 GGVEHAEYCFQHYTSSETCFSAFKAPLDPSTSLGGFFGSNYSEASAFVITYPVNNAIDAV 565
Query: 541 GNENGKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSY 600
GNENGKA+AWEKAFVKL KEEL+PLV SRNLTLSFSSESSIEEELKRESTAD+LTI VSY
Sbjct: 566 GNENGKAIAWEKAFVKLAKEELMPLVHSRNLTLSFSSESSIEEELKRESTADILTIAVSY 625
Query: 601 LVMFVYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIME 660
LVMF YISVALGDS+ISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIG+KSTLIIME
Sbjct: 626 LVMFAYISVALGDSNISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGIKSTLIIME 685
Query: 661 VIPFLVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGA 720
VIPFLVLAVGVDNMCILVHAVKRQP+EL+LE+RISSALVEVGPSITLASLSEILAFAVG
Sbjct: 686 VIPFLVLAVGVDNMCILVHAVKRQPYELSLEDRISSALVEVGPSITLASLSEILAFAVGT 745
Query: 721 FVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEP 780
FVPMPACRVFS+FAALAVLLDFILQLSAFVALIV DILRAEDHRVDCFPCIKV PHSDEP
Sbjct: 746 FVPMPACRVFSLFAALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCIKVHPHSDEP 805
Query: 781 NQGFNQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIV 840
NQGFNQG+ GLLS YMKDVHAPLLG WGVKI V+VIFVG TLG IALSTKIEVGLEQKIV
Sbjct: 806 NQGFNQGRHGLLSRYMKDVHAPLLGFWGVKIVVVVIFVGLTLGSIALSTKIEVGLEQKIV 865
Query: 841 LPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISR 900
LPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSS SRQTNQLCSIS CDSNSLLNEISR
Sbjct: 866 LPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSRSRQTNQLCSISHCDSNSLLNEISR 925
Query: 901 ASLTPELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDS 960
ASLTPELNYIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSYCPPDDQPPCCFPDEG CDS
Sbjct: 926 ASLTPELNYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCFPDEGFCDS 985
Query: 961 SGGVCKDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGY 1020
S GVC+DCTTCF +SDLV RPTT QF+EKLPWFLNSLPSADCAKGGHGAYTNSVNL GY
Sbjct: 986 SEGVCRDCTTCFRHSDLVGDRPTTGQFKEKLPWFLNSLPSADCAKGGHGAYTNSVNLKGY 1045
Query: 1021 ESGIIKASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLD 1080
E GIIKASEFR+YHTPLNKQGDYVNALRAAKEF SK+SDSLKMD+FPYSVFYIFFEQYLD
Sbjct: 1046 EIGIIKASEFRSYHTPLNKQGDYVNALRAAKEFCSKISDSLKMDIFPYSVFYIFFEQYLD 1105
Query: 1081 IWKIALINIAVALG-----------YLWSSGIIILVLAMIIIDLMGIMAILNIQLNAVSV 1140
IW AL+NIA+ALG LW SG+IILVLAMI+IDL+G+MAIL IQLNAVSV
Sbjct: 1106 IWNTALMNIAIALGAIFIVSLVITSSLWCSGMIILVLAMIVIDLLGVMAILKIQLNAVSV 1165
Query: 1141 VNLLMSIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1168
VN+LMSIGIAVEFCVH+VHAF VS GDR+QRA+EAL T+GASV
Sbjct: 1166 VNILMSIGIAVEFCVHLVHAFSVSCGDRNQRAQEALSTIGASV 1208
BLAST of MS004994 vs. TAIR 10
Match:
AT4G38350.1 (Patched family protein )
HSP 1 Score: 1702.2 bits (4407), Expect = 0.0e+00
Identity = 857/1178 (72.75%), Postives = 991/1178 (84.13%), Query Frame = 0
Query: 2 RHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFD 61
RHS EYCAMYDICG R DGKVLNCPY SPS++PDELFS KIQSLCPTISGNVCCTE QFD
Sbjct: 32 RHSKEYCAMYDICGHRSDGKVLNCPYASPSIQPDELFSAKIQSLCPTISGNVCCTETQFD 91
Query: 62 TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDYY 121
TLRSQVQQA+P VGCPACLRNFLNLFCELSCSP QSLFINVTS++EV GN+TVDGIDY+
Sbjct: 92 TLRSQVQQAVPFLVGCPACLRNFLNLFCELSCSPNQSLFINVTSVAEVSGNLTVDGIDYH 151
Query: 122 VTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTINF 181
+T+ FG+GLY+SCK+VKFGTMNTRAI+FVGGGAK+F E F F+G+KA GFPGSPY INF
Sbjct: 152 ITDTFGEGLYESCKEVKFGTMNTRAINFVGGGAKNFREWFTFIGQKAPSGFPGSPYAINF 211
Query: 182 KLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFLE 241
K + P+SS M MNVSVYSCGDTSLGCSCGDCP SP CSS EP P ++CSIRI L+
Sbjct: 212 KSSIPESSAMVPMNVSVYSCGDTSLGCSCGDCPSSPACSSPEPLPPHDEDSCSIRIGPLK 271
Query: 242 VGCIDFSITIIYIIFISAFLGWALFHPARERRFSA-REEPLLNIGDGHEISSVNLEENEY 301
V CI+ S+ ++Y++ +S F GWA + R +PLL H + E+
Sbjct: 272 VRCIELSMALVYVLLVSCFFGWAGLNRRRNTTQPLDSSKPLL-----HPVE----EDGIN 331
Query: 302 SATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLVR 361
S K + + ++ QLS +Q Y++ FYR+YG+W+ARNP LVLF S++IVL LC GL
Sbjct: 332 SEMKENILGVKVQRHAQLSPVQRYMAKFYRSYGSWIARNPSLVLFMSVAIVLALCSGLYN 391
Query: 362 FKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDNI 421
FKVET+PEKLWVG SKAA EK+FFD+ L+PFYRIEQ+I+AT P+ + GRAP IVT++NI
Sbjct: 392 FKVETRPEKLWVGPESKAAEEKKFFDTHLSPFYRIEQLILATVPDPKSGRAPSIVTDENI 451
Query: 422 LLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQH 481
LLLFDIQ KV+++ NYSGS VSL DICLKPLGEDCATQSILQYFKMD +DDYGGV+H
Sbjct: 452 LLLFDIQQKVDQIRGNYSGSEVSLPDICLKPLGEDCATQSILQYFKMDSGTFDDYGGVEH 511
Query: 482 AEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENG 541
AEYCFQHY S+ETC SAF+AP+DPS LGGFSGNNYSEA+AFVVTYPVNN I NEN
Sbjct: 512 AEYCFQHYTSSETCLSAFQAPVDPSAVLGGFSGNNYSEATAFVVTYPVNNVIGDSSNENA 571
Query: 542 KAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFV 601
+AVAWEK+F++L KEELLP+V+S+NL+LSFSSESSIEEELKRESTADV+TI SYLVMFV
Sbjct: 572 RAVAWEKSFIQLAKEELLPMVRSKNLSLSFSSESSIEEELKRESTADVITIAASYLVMFV 631
Query: 602 YISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFL 661
YISV LGD+ +FY+SSKVLLGLSGV+LV+LSVLGSVG FSA+GVKSTLIIMEVIPFL
Sbjct: 632 YISVTLGDAPQFYTFYISSKVLLGLSGVVLVLLSVLGSVGVFSALGVKSTLIIMEVIPFL 691
Query: 662 VLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMP 721
VLAVGVDNMCILVHAVKRQP E++LE+RISSALVEVGPSITLASLSE+LAFAVGAFVPMP
Sbjct: 692 VLAVGVDNMCILVHAVKRQPREVSLEQRISSALVEVGPSITLASLSEVLAFAVGAFVPMP 751
Query: 722 ACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFN 781
ACR+FSMFAALA++LDF LQ++AFVALIVFD R+ D+R+DCFPCIKV S E +G
Sbjct: 752 ACRIFSMFAALAIMLDFFLQITAFVALIVFDCKRSADNRIDCFPCIKVPSSSRESVEGGR 811
Query: 782 QGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDS 841
+ PG L YMK+VHAP+LGLWGVK+ V+ +F F L IA+S ++E GLEQKIVLPRDS
Sbjct: 812 E--PGFLERYMKEVHAPVLGLWGVKMVVVAVFFAFALASIAISPRLETGLEQKIVLPRDS 871
Query: 842 YLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLTP 901
YLQDYFD L+EYLR+GPPLYFVVK+YNYSSESR TNQLCSISQC+SNSLLNEISRAS
Sbjct: 872 YLQDYFDSLSEYLRVGPPLYFVVKNYNYSSESRHTNQLCSISQCNSNSLLNEISRASQAS 931
Query: 902 ELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGVC 961
+ +YIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSYCPPDDQPPCC +E C S G+C
Sbjct: 932 DTSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSYCPPDDQPPCCTAEEDIC-SLDGIC 991
Query: 962 KDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGII 1021
KDCTTCF +SDLV RP+T QF+EKLPWFLN+LPSADCAKGGHGAYTNSV+L GYESG+I
Sbjct: 992 KDCTTCFRHSDLVQDRPSTAQFREKLPWFLNALPSADCAKGGHGAYTNSVDLKGYESGVI 1051
Query: 1022 KASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLDIWKIA 1081
+ASEFRTYHTPLN QGDYVNALRAA+EFSS++S+SLK+D+FPYSVFYIFFEQYL+IW +A
Sbjct: 1052 QASEFRTYHTPLNTQGDYVNALRAAREFSSRISNSLKIDIFPYSVFYIFFEQYLNIWTVA 1111
Query: 1082 LINIAVALGYL-----------WSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLM 1141
L N+A+A+G + WSS II+LVL MI++DLMG+M IL IQLNAVSVVNL+M
Sbjct: 1112 LTNLAIAIGAIFIVCWLITSSAWSSAIIVLVLVMILVDLMGMMVILGIQLNAVSVVNLIM 1171
Query: 1142 SIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGASV 1168
SIGIAVEFCVHI HAFL+SSGDR RAREAL TMGASV
Sbjct: 1172 SIGIAVEFCVHISHAFLMSSGDREHRAREALETMGASV 1197
BLAST of MS004994 vs. TAIR 10
Match:
AT4G38350.2 (Patched family protein )
HSP 1 Score: 1688.7 bits (4372), Expect = 0.0e+00
Identity = 857/1202 (71.30%), Postives = 991/1202 (82.45%), Query Frame = 0
Query: 2 RHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFD 61
RHS EYCAMYDICG R DGKVLNCPY SPS++PDELFS KIQSLCPTISGNVCCTE QFD
Sbjct: 32 RHSKEYCAMYDICGHRSDGKVLNCPYASPSIQPDELFSAKIQSLCPTISGNVCCTETQFD 91
Query: 62 TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDYY 121
TLRSQVQQA+P VGCPACLRNFLNLFCELSCSP QSLFINVTS++EV GN+TVDGIDY+
Sbjct: 92 TLRSQVQQAVPFLVGCPACLRNFLNLFCELSCSPNQSLFINVTSVAEVSGNLTVDGIDYH 151
Query: 122 VTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTINF 181
+T+ FG+GLY+SCK+VKFGTMNTRAI+FVGGGAK+F E F F+G+KA GFPGSPY INF
Sbjct: 152 ITDTFGEGLYESCKEVKFGTMNTRAINFVGGGAKNFREWFTFIGQKAPSGFPGSPYAINF 211
Query: 182 KLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFLE 241
K + P+SS M MNVSVYSCGDTSLGCSCGDCP SP CSS EP P ++CSIRI L+
Sbjct: 212 KSSIPESSAMVPMNVSVYSCGDTSLGCSCGDCPSSPACSSPEPLPPHDEDSCSIRIGPLK 271
Query: 242 VGCIDFSITIIYIIFISAFLGWALFHPARERRFSA-REEPLLNIGDGHEISSVNLEENEY 301
V CI+ S+ ++Y++ +S F GWA + R +PLL H + E+
Sbjct: 272 VRCIELSMALVYVLLVSCFFGWAGLNRRRNTTQPLDSSKPLL-----HPVE----EDGIN 331
Query: 302 SATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLVR 361
S K + + ++ QLS +Q Y++ FYR+YG+W+ARNP LVLF S++IVL LC GL
Sbjct: 332 SEMKENILGVKVQRHAQLSPVQRYMAKFYRSYGSWIARNPSLVLFMSVAIVLALCSGLYN 391
Query: 362 FKVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDNI 421
FKVET+PEKLWVG SKAA EK+FFD+ L+PFYRIEQ+I+AT P+ + GRAP IVT++NI
Sbjct: 392 FKVETRPEKLWVGPESKAAEEKKFFDTHLSPFYRIEQLILATVPDPKSGRAPSIVTDENI 451
Query: 422 LLLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSILQYFKMDPENYDDYGGVQH 481
LLLFDIQ KV+++ NYSGS VSL DICLKPLGEDCATQSILQYFKMD +DDYGGV+H
Sbjct: 452 LLLFDIQQKVDQIRGNYSGSEVSLPDICLKPLGEDCATQSILQYFKMDSGTFDDYGGVEH 511
Query: 482 AEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSE---------------------- 541
AEYCFQHY S+ETC SAF+AP+DPS LGGFSGNNYSE
Sbjct: 512 AEYCFQHYTSSETCLSAFQAPVDPSAVLGGFSGNNYSEVMVSELGCSVPFDCYSDVKRTL 571
Query: 542 --ASAFVVTYPVNNAIDAVGNENGKAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSI 601
A+AFVVTYPVNN I NEN +AVAWEK+F++L KEELLP+V+S+NL+LSFSSESSI
Sbjct: 572 FQATAFVVTYPVNNVIGDSSNENARAVAWEKSFIQLAKEELLPMVRSKNLSLSFSSESSI 631
Query: 602 EEELKRESTADVLTIVVSYLVMFVYISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVL 661
EEELKRESTADV+TI SYLVMFVYISV LGD+ +FY+SSKVLLGLSGV+LV+LSVL
Sbjct: 632 EEELKRESTADVITIAASYLVMFVYISVTLGDAPQFYTFYISSKVLLGLSGVVLVLLSVL 691
Query: 662 GSVGFFSAIGVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPFELTLEERISSALVEV 721
GSVG FSA+GVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQP E++LE+RISSALVEV
Sbjct: 692 GSVGVFSALGVKSTLIIMEVIPFLVLAVGVDNMCILVHAVKRQPREVSLEQRISSALVEV 751
Query: 722 GPSITLASLSEILAFAVGAFVPMPACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAE 781
GPSITLASLSE+LAFAVGAFVPMPACR+FSMFAALA++LDF LQ++AFVALIVFD R+
Sbjct: 752 GPSITLASLSEVLAFAVGAFVPMPACRIFSMFAALAIMLDFFLQITAFVALIVFDCKRSA 811
Query: 782 DHRVDCFPCIKVTPHSDEPNQGFNQGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFT 841
D+R+DCFPCIKV S E +G + PG L YMK+VHAP+LGLWGVK+ V+ +F F
Sbjct: 812 DNRIDCFPCIKVPSSSRESVEGGRE--PGFLERYMKEVHAPVLGLWGVKMVVVAVFFAFA 871
Query: 842 LGGIALSTKIEVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTN 901
L IA+S ++E GLEQKIVLPRDSYLQDYFD L+EYLR+GPPLYFVVK+YNYSSESR TN
Sbjct: 872 LASIAISPRLETGLEQKIVLPRDSYLQDYFDSLSEYLRVGPPLYFVVKNYNYSSESRHTN 931
Query: 902 QLCSISQCDSNSLLNEISRASLTPELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSY 961
QLCSISQC+SNSLLNEISRAS + +YIAKPAASWLDDFLVWLSP+AFGCCRKFTNGSY
Sbjct: 932 QLCSISQCNSNSLLNEISRASQASDTSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGSY 991
Query: 962 CPPDDQPPCCFPDEGSCDSSGGVCKDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSA 1021
CPPDDQPPCC +E C S G+CKDCTTCF +SDLV RP+T QF+EKLPWFLN+LPSA
Sbjct: 992 CPPDDQPPCCTAEEDIC-SLDGICKDCTTCFRHSDLVQDRPSTAQFREKLPWFLNALPSA 1051
Query: 1022 DCAKGGHGAYTNSVNLNGYESGIIKASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSL 1081
DCAKGGHGAYTNSV+L GYESG+I+ASEFRTYHTPLN QGDYVNALRAA+EFSS++S+SL
Sbjct: 1052 DCAKGGHGAYTNSVDLKGYESGVIQASEFRTYHTPLNTQGDYVNALRAAREFSSRISNSL 1111
Query: 1082 KMDVFPYSVFYIFFEQYLDIWKIALINIAVALGYL-----------WSSGIIILVLAMII 1141
K+D+FPYSVFYIFFEQYL+IW +AL N+A+A+G + WSS II+LVL MI+
Sbjct: 1112 KIDIFPYSVFYIFFEQYLNIWTVALTNLAIAIGAIFIVCWLITSSAWSSAIIVLVLVMIL 1171
Query: 1142 IDLMGIMAILNIQLNAVSVVNLLMSIGIAVEFCVHIVHAFLVSSGDRSQRAREALGTMGA 1168
+DLMG+M IL IQLNAVSVVNL+MSIGIAVEFCVHI HAFL+SSGDR RAREAL TMGA
Sbjct: 1172 VDLMGMMVILGIQLNAVSVVNLIMSIGIAVEFCVHISHAFLMSSGDREHRAREALETMGA 1221
BLAST of MS004994 vs. TAIR 10
Match:
AT1G42470.1 (Patched family protein )
HSP 1 Score: 1593.6 bits (4125), Expect = 0.0e+00
Identity = 802/1156 (69.38%), Postives = 959/1156 (82.96%), Query Frame = 0
Query: 2 RHSTEYCAMYDICGARGDGKVLNCPYGSPSVKPDELFSEKIQSLCPTISGNVCCTEAQFD 61
+ S YCAMYDICGAR DGKVLNCP+ PSVKPD+L S KIQSLCPTI+GNVCCTE QFD
Sbjct: 13 KQSAGYCAMYDICGARSDGKVLNCPFNIPSVKPDDLLSSKIQSLCPTITGNVCCTETQFD 72
Query: 62 TLRSQVQQAIPLFVGCPACLRNFLNLFCELSCSPRQSLFINVTSISEVGGNMTVDGIDYY 121
TLRSQVQQAIP VGCPACLRNFLNLFCEL+CSP QSLFINVTS ++V N TVDGI YY
Sbjct: 73 TLRSQVQQAIPFIVGCPACLRNFLNLFCELTCSPDQSLFINVTSTTKVKNNSTVDGIQYY 132
Query: 122 VTEKFGKGLYDSCKDVKFGTMNTRAIDFVGGGAKSFEELFAFLGKKAAPGFPGSPYTINF 181
+T+ FG G+Y+SCK+VKFG+ N+RA+DF+G GAK+F+E F F+G+KA PGSPY I F
Sbjct: 133 ITDDFGAGMYESCKNVKFGSSNSRALDFLGAGAKNFKEWFTFIGQKAGVNLPGSPYGIAF 192
Query: 182 KLNTPKSSPMELMNVSVYSCGDTSLGCSCGDCPLSPVCSSLEPPSPPKSNACSIRIWFLE 241
P SS M MNVS+YSCGD SLGCSCGDCP + CSS K ++CSI+I LE
Sbjct: 193 LPTPPVSSGMRPMNVSIYSCGDESLGCSCGDCPSAATCSSKAEVPTQKKHSCSIKIGSLE 252
Query: 242 VGCIDFSITIIYIIFISAFLGWALFHPARERRFSAREEPLLNIGDGHEISSVNLEENEYS 301
V C+DF + I+YI+ +S FLG L HP R ++ +++ L E +SVN ++ +
Sbjct: 253 VKCVDFILAILYIVLVSLFLGGGLLHPVRGKKKTSQMGTLSEASG--ERNSVNQQKPDTI 312
Query: 302 ATKVGLDVLTLRNGIQLSTIQGYISNFYRNYGAWVARNPILVLFSSLSIVLILCVGLVRF 361
+++ L RN QLST+QG+++NFY YG WVAR+P LVL S+S+VL+LCVGL+RF
Sbjct: 313 QSQM-LQNTPQRNWGQLSTVQGHLANFYGKYGIWVARHPTLVLCLSVSVVLLLCVGLIRF 372
Query: 362 KVETQPEKLWVGHGSKAAAEKQFFDSQLAPFYRIEQMIIATKPEERHGRAPRIVTEDNIL 421
KVET+P+KLWVG GS+AA EKQFFD+ LAPFYRIEQ+IIAT H +AP I+T+DNI
Sbjct: 373 KVETRPDKLWVGSGSRAAEEKQFFDTHLAPFYRIEQLIIATVQTSSHEKAPEILTDDNIK 432
Query: 422 LLFDIQDKVNELVANYSGSLVSLNDICLKPLGEDCATQSIL-QYFKMDPENYDDYGGVQH 481
LLFDIQ KV+ L AN+SGS+VSL DIC+KPLGEDCATQS+L QYFKM PENYDDYGGV H
Sbjct: 433 LLFDIQKKVDGLRANHSGSMVSLTDICMKPLGEDCATQSVLQQYFKMKPENYDDYGGVDH 492
Query: 482 AEYCFQHYASTETCFSAFKAPLDPSTSLGGFSGNNYSEASAFVVTYPVNNAIDAVGNENG 541
+YCF+H+ STE+C SAFK PLDP+T+LGGFSGN++SEASAF+VTYPV+N +D GN+
Sbjct: 493 VKYCFEHFTSTESCLSAFKGPLDPTTALGGFSGNSFSEASAFLVTYPVDNFVDNKGNKTE 552
Query: 542 KAVAWEKAFVKLVKEELLPLVQSRNLTLSFSSESSIEEELKRESTADVLTIVVSYLVMFV 601
KAVAWEKAF++L K+ELLP+VQ++NLTLSFSSESSIEEELKRESTADV+TI +SYLVMF
Sbjct: 553 KAVAWEKAFIQLAKDELLPMVQAKNLTLSFSSESSIEEELKRESTADVITIAISYLVMFA 612
Query: 602 YISVALGDSSISSSFYLSSKVLLGLSGVILVVLSVLGSVGFFSAIGVKSTLIIMEVIPFL 661
YIS+ LGDS SFY++SKVLLGLSGV+LV+LSVLGSVGFFSA+G+KSTLIIMEVIPFL
Sbjct: 613 YISLTLGDSPRLKSFYITSKVLLGLSGVLLVMLSVLGSVGFFSAVGMKSTLIIMEVIPFL 672
Query: 662 VLAVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMP 721
VLAVGVDNMCILVHAVKRQ EL LE RIS+AL+EVGPSITLASL+EILAFAVGAF+ MP
Sbjct: 673 VLAVGVDNMCILVHAVKRQEQELPLERRISNALMEVGPSITLASLAEILAFAVGAFIKMP 732
Query: 722 ACRVFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFN 781
A RVFSMFAALAVLLDF+LQ++AFVALIVFD R ED RVDCFPCIK + S +G
Sbjct: 733 AVRVFSMFAALAVLLDFLLQITAFVALIVFDFRRTEDKRVDCFPCIKTSKSSISAEKGVG 792
Query: 782 QGKPGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDS 841
Q K GLL+ YMK+VHAP+L W VKI VI F G + GIALST+IE GLEQ+IVLP+DS
Sbjct: 793 QRKAGLLTRYMKEVHAPVLSHWIVKIVVIAFFFGLAMAGIALSTRIEPGLEQQIVLPQDS 852
Query: 842 YLQDYFDDLAEYLRIGPPLYFVVKDYNYSSESRQTNQLCSISQCDSNSLLNEISRASLTP 901
YLQ YF++++ YLRIGPPLYFV+K+YNYSSESR TNQLCSI++C+ NSLLNEI+RASLTP
Sbjct: 853 YLQGYFNNISTYLRIGPPLYFVLKNYNYSSESRHTNQLCSINKCNPNSLLNEIARASLTP 912
Query: 902 ELNYIAKPAASWLDDFLVWLSPDAFGCCRKFTNGSYCPPDDQPPCCFPDEGSCDSSGGVC 961
EL+YIAKPAASWLDDFLVWLSP+AFGCCRKFTNG++CPPDDQPPCC P + SC S VC
Sbjct: 913 ELSYIAKPAASWLDDFLVWLSPEAFGCCRKFTNGTFCPPDDQPPCCPPGQTSCGLS-EVC 972
Query: 962 KDCTTCFHYSDLVAGRPTTIQFQEKLPWFLNSLPSADCAKGGHGAYTNSVNLNGYESGII 1021
KDCTTCF ++DL + RP+T QF+EKLPWFLN+LPSADCAKGGHGAY++SV+L GY +GII
Sbjct: 973 KDCTTCFRHADLSSDRPSTTQFKEKLPWFLNALPSADCAKGGHGAYSSSVDLQGYANGII 1032
Query: 1022 KASEFRTYHTPLNKQGDYVNALRAAKEFSSKMSDSLKMDVFPYSVFYIFFEQYLDIWKIA 1081
+AS FRTYHTPLNKQ D+VN++RAA+EFS+K+S SLKM+++PYSVFY+FFEQYLDIWK A
Sbjct: 1033 QASSFRTYHTPLNKQVDFVNSMRAAQEFSAKVSRSLKMEIYPYSVFYMFFEQYLDIWKTA 1092
Query: 1082 LINIAVALGYL-----------WSSGIIILVLAMIIIDLMGIMAILNIQLNAVSVVNLLM 1141
LIN+++A+ + WSS II+LV+AMIIIDL+G+MA+ +IQLNA+SVVNL+M
Sbjct: 1093 LINLSIAIAAVFVVCLIITCSFWSSAIILLVIAMIIIDLLGVMAVFHIQLNALSVVNLIM 1152
Query: 1142 SIGIAVEFCVHIVHAF 1146
S+GIAVEFCVHI HAF
Sbjct: 1153 SVGIAVEFCVHITHAF 1164
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| P56941 | 2.9e-176 | 34.69 | NPC intracellular cholesterol transporter 1 OS=Sus scrofa OX=9823 GN=NPC1 PE=2 S... | [more] |
| O35604 | 6.4e-176 | 34.31 | NPC intracellular cholesterol transporter 1 OS=Mus musculus OX=10090 GN=Npc1 PE=... | [more] |
| O15118 | 2.4e-175 | 34.85 | NPC intracellular cholesterol transporter 1 OS=Homo sapiens OX=9606 GN=NPC1 PE=1... | [more] |
| Q6T3U3 | 3.1e-146 | 32.67 | NPC1-like intracellular cholesterol transporter 1 OS=Rattus norvegicus OX=10116 ... | [more] |
| Q9UHC9 | 7.6e-145 | 32.19 | NPC1-like intracellular cholesterol transporter 1 OS=Homo sapiens OX=9606 GN=NPC... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CI77 | 0.0e+00 | 98.30 | Niemann-Pick C1 protein OS=Momordica charantia OX=3673 GN=LOC111011166 PE=3 SV=1 | [more] |
| A0A6J1I473 | 0.0e+00 | 89.31 | Niemann-Pick C1 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111469740 PE=3 SV... | [more] |
| A0A6J1G6Q4 | 0.0e+00 | 89.48 | Niemann-Pick C1 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111451262 PE=3 ... | [more] |
| A0A1S3BJR3 | 0.0e+00 | 88.04 | Niemann-Pick C1 protein-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490460 ... | [more] |
| A0A5A7U0V7 | 0.0e+00 | 87.83 | Niemann-Pick C1 protein-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 G... | [more] |
