MS027902 (gene) Bitter gourd (TR) v1

Overview


Name	MS027902
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	disease resistance protein RGA2-like
Location	scaffold375: 24009 .. 27152 (-)
RNA-Seq Expression	MS027902
Synteny	MS027902

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAGTTGCTATTGACTTTTGCTGTAGAGGGAGTGTTAAGCAAAGTGGTGAAAGTTGCAACTGAGTTAATTGGGCTCGCATGGGGCCTGGAAAAGGAGCTAGAGGAGCTCAAGAAATGGCTACTCAACATTGAAGCATTCTTATGTGATATCAACAAAAGCAAATTTCATCATAATTCAGTGAAGCTGTGGGTGGACGAACTTCAAGATCTTGTTCATGAAGCTGAGAATCCATTGGACGAGCTTGTTTATGAGCATCTTCGGCGAAAGGTGCAAACAGGAAAAAGGAACAACGTACGTGATTTTGCATCCTCTTCTAATAATCCTTTGGTCTTTCCTGTCAAGATGGCAAATAGAATAAAGAACATTACGAACCAGTTGCAAAAGCATTATGATAGGGCAGGTCATTTGGGAATTGTTGGCAGTGGATCCGCAGGAACAGAGACTGTTATTAGCCAAATTCGACAGACAGTCTCGGAGTTGGATGACTTTGGAGTTGTGGGGAGGGATGTTGAAGTCTCTAGCATAGTGAAGCAGGTGGTGGATGCTAGAAATAGTCATGTTACATCTATCTTACCCATTGTAGGTGTGGGTGGGCTGGGAAAAACAACGTTGGCAAAGTTGGTCTTCAATCACCCGAGGATCAAACAACATTTTGATAAAACTATATGGGTGTGTGTCCGAACCTTTTGATATCAACAAGATTTTGGAAGCATTCTTACAAAACTTAAAAGGTCCTTCAGGTGCCTGCGATAACAGGGAGGTATTGCTTCGTGAACTTAAAAAGGAAATGGGTGACAAAAGATATTTTCTTGTGCTTGATGATGTTTAGAATGAAAATTCTTCTTTGTGGGATGAGTTGAAGAATTGTTTGATGAGGATGACTGAGAAATCAGAAAATAGTATTGTTGTTACTACACGCAGCGTTGAAGTTGCCAAAATCATGGAAACACTTCCTAGTCATCATTTGAGTAAGCTCTCCGTCGATCAGTGTTGGTCCTTATTTCAAGAAAGTGCAAATGCAAATGGACTACCAATGACTTTAAGTTTGGAAGTTGTTCGAGAAAGGTTGGTTAAAAAAATTGGTGGCATACCATTAGTGGCTAAAGTTTGGGGGAAGACAGTACAATTTCAAGGAAACTATGATGGATGGTTAAAAAAATTGGTGGCATACCATTAGTGGCTAAAGTTTGGGGGAAGACAGTACAATTTCAAGGAAACTATGATGGATGGGTGACGAAATTGGAGAGTGAAATAAATGAAATGAGAACTCCAACTCAAGAGGAAAAATGTGTTTTAGAAATATTGAAATTAAGTGTGGATCGCCTACCATCATTTTCATTAAAGCAATGTTTTGCCTACTGTTCAAATTTTCCCAAAGATTTTGTGTTTGAAAAAGAACAACTAATTCTTATGTGGATAGCACAAGGGTTTATTTATCTACAAGAAAGAAGTAGCAACGAAACAATGGAAGACGAAGGAGATAGGCACTTCAAGATTTTGTTGTCTCGTTGCTTATTTCAAGATGTTGTTAAAGATAGCACAGGAAGAATTATAAGATGTAAGATGCATGATCTTTTACATAAAATTGCATGTGATGTCTCAAATGATCTTAAATTACAATATTTTATCAATTCATTGGATGCGGAACATTGGACGAATGAGAATCAAAACATTGCAAGCAAGTTACGCACCATAATTATTTATGATCAAAAGATGATGCCTTACAAGCAAGGGGAGGTGATTCGTGATAAGATGATAAACTTTGTTTGTTTGCGTGTTTTGGTGATGAATTTGAAGTCTATCTATGAGTTACCAGATTCAATTAGTAAGTTGAAACACCTGAGATATCTCGACATTTCAAATTGTTCAATAAAAAAGCTTCCAGAATCTATTGGCGTGCTTTATAATTTGCAAACACTAAGGATGACATGGACGCCATCGGTAGATTTTCCAAAGAATTTTGGAAAATTAGTTAGTTTGAGACATTTGGAATTTCTTTTTTCCATTTCTGGATCAACGAGACATTTGGAATTGCCAAATAATAGACCTGCATGTCTGAGCAAACTGATTCAACTTCAAACATTGCCGCGTTTTCCAGTAGGGTTTGAGAATGGTTGTAAAATTACAGAACTTGGACCTTTGAAAAACCTGAGAGGTCGTTTGGCTCTTCACTATCTAGATCGAGTTGAAAGTAAAGAGGAAGCCAAGACTGCAAAATTGGTGGAAAAGGAGAACTTACACGAGCTAACCTTTTATTGGAGTCCGCTCCATGAAAGGAAACATGAGGATCATAATCACAACGATTTTGAATTGTTGGAAGGACTTCAACCTCCCAAAAGTCTACAAATTTTGACGATCGAGTCCTTTTTAGGCCAATGTTTTCCTAACAATACTTTTGTTGAGAATTTAGTGTCAGTACTTCTATCTTCTTGTTCAAATTGTGAAAAGCTTCCAATGCTTGGGCATCTAGCCAACCTAGAGATCCTTGGTATTTCTGGGTTACACAAGATACAAAGTATAGGCAATGAATTCTATGGAATTAATGACTCCAACAAAAAGCCAATATTGTTCCCCAAGTTGAAGAGATTTGAACTCGAAGATATGAGAAGTCTAGTGCAGTGGGAAGAAGTGACAGTCGCATCAAATGTTACCATTTTTCCTCGTCTTGAAGACTTAAAAATTAGATCCTGTGAAAAATTATTGAGCATTCCAAATGTTTTTGGTTCCTGTGATGAGAATGGTGACATGCACCTTAAAACTGTTACTATCAGTGACTGTGGAGTATTAGGCGAACTTCCTGATGGACTAAAATATTGTGATTCCATCCAAGATTTGAGTATATTCCGCTGCTCTTGTTTGGCCGTGATATTGGAAAGAAGTGGCATGGTTTATTCTTTAACATTCGTGTTGCCGCAGAAGTTAAAGGACCGCAGCGACTTCGTAATAGGAATTCGTGGAAGCTTCCTTAGGGATGATTATATTCTTGATGAAGAGCAGTTAGATTTGGTTGATGAGTTGGAAATTGCGAGCCTAGAACCATGGATTCGAGAATGTGGTTGCCTCCAAACTTTGCCAGAATGGTTAGTAAATCTCAAGTCTTTGCAAACATTGGATCTTACCTATGTCGTCCTTACCTAG

mRNA sequence

ATGGCTGAGTTGCTATTGACTTTTGCTGTAGAGGGAGTGTTAAGCAAAGTGGTGAAAGTTGCAACTGAGTTAATTGGGCTCGCATGGGGCCTGGAAAAGGAGCTAGAGGAGCTCAAGAAATGGCTACTCAACATTGAAGCATTCTTATGTGATATCAACAAAAGCAAATTTCATCATAATTCAGTGAAGCTGTGGGTGGACGAACTTCAAGATCTTGTTCATGAAGCTGAGAATCCATTGGACGAGCTTGTTTATGAGCATCTTCGGCGAAAGGTGCAAACAGGAAAAAGGAACAACGTACGTGATTTTGCATCCTCTTCTAATAATCCTTTGGTCTTTCCTGTCAAGATGGCAAATAGAATAAAGAACATTACGAACCAGTTGCAAAAGCATTATGATAGGGCAGGTCATTTGGGAATTGTTGGCAGTGGATCCGCAGGAACAGAGACTGTTATTAGCCAAATTCGACAGACAGTCTCGGAGTTGGATGACTTTGGAGTTGTGGGGAGGGATGTTGAAGTCTCTAGCATAGTGAAGCAGGTGGTGGATGCTAGAAATAGTCATGTTACATCTATCTTACCCATTGTAGGTGTGGGTGGGCTGGGAAAAACAACGTTGGCAAAGTTGGTCTTCAATCACCCGAGGATCAAACAACATTTTGATAAAACTATATGGAATGAAAATTCTTCTTTGTGGGATGAGTTGAAGAATTGTTTGATGAGGATGACTGAGAAATCAGAAAATAGTATTGTTGTTACTACACGCAGCGTTGAAGTTGCCAAAATCATGGAAACACTTCCTAGTCATCATTTGAGTAAGCTCTCCGTCGATCAGTGTTGGTCCTTATTTCAAGAAAGTGCAAATGCAAATGGACTACCAATGACTTTAAGTTTGGAAGTTGTTCGAGAAAGCATTCCAAATGTTTTTGGTTCCTGTGATGAGAATGGTGACATGCACCTTAAAACTGTTACTATCAGTGACTGTGGAGTATTAGGCGAACTTCCTGATGGACTAAAATATTGTGATTCCATCCAAGATTTGAGTATATTCCGCTGCTCTTGTTTGGCCGTGATATTGGAAAGAAGTGGCATGGTTTATTCTTTAACATTCGTGTTGCCGCAGAAGTTAAAGGACCGCAGCGACTTCGTAATAGGAATTCGTGGAAGCTTCCTTAGGGATGATTATATTCTTGATGAAGAGCAGTTAGATTTGGTTGATGAGTTGGAAATTGCGAGCCTAGAACCATGGATTCGAGAATGTGGTTGCCTCCAAACTTTGCCAGAATGGTTAGTAAATCTCAAGTCTTTGCAAACATTGGATCTTACCTATGTCGTCCTTACCTAG

Coding sequence (CDS)

ATGGCTGAGTTGCTATTGACTTTTGCTGTAGAGGGAGTGTTAAGCAAAGTGGTGAAAGTTGCAACTGAGTTAATTGGGCTCGCATGGGGCCTGGAAAAGGAGCTAGAGGAGCTCAAGAAATGGCTACTCAACATTGAAGCATTCTTATGTGATATCAACAAAAGCAAATTTCATCATAATTCAGTGAAGCTGTGGGTGGACGAACTTCAAGATCTTGTTCATGAAGCTGAGAATCCATTGGACGAGCTTGTTTATGAGCATCTTCGGCGAAAGGTGCAAACAGGAAAAAGGAACAACGTACGTGATTTTGCATCCTCTTCTAATAATCCTTTGGTCTTTCCTGTCAAGATGGCAAATAGAATAAAGAACATTACGAACCAGTTGCAAAAGCATTATGATAGGGCAGGTCATTTGGGAATTGTTGGCAGTGGATCCGCAGGAACAGAGACTGTTATTAGCCAAATTCGACAGACAGTCTCGGAGTTGGATGACTTTGGAGTTGTGGGGAGGGATGTTGAAGTCTCTAGCATAGTGAAGCAGGTGGTGGATGCTAGAAATAGTCATGTTACATCTATCTTACCCATTGTAGGTGTGGGTGGGCTGGGAAAAACAACGTTGGCAAAGTTGGTCTTCAATCACCCGAGGATCAAACAACATTTTGATAAAACTATATGGAATGAAAATTCTTCTTTGTGGGATGAGTTGAAGAATTGTTTGATGAGGATGACTGAGAAATCAGAAAATAGTATTGTTGTTACTACACGCAGCGTTGAAGTTGCCAAAATCATGGAAACACTTCCTAGTCATCATTTGAGTAAGCTCTCCGTCGATCAGTGTTGGTCCTTATTTCAAGAAAGTGCAAATGCAAATGGACTACCAATGACTTTAAGTTTGGAAGTTGTTCGAGAAAGCATTCCAAATGTTTTTGGTTCCTGTGATGAGAATGGTGACATGCACCTTAAAACTGTTACTATCAGTGACTGTGGAGTATTAGGCGAACTTCCTGATGGACTAAAATATTGTGATTCCATCCAAGATTTGAGTATATTCCGCTGCTCTTGTTTGGCCGTGATATTGGAAAGAAGTGGCATGGTTTATTCTTTAACATTCGTGTTGCCGCAGAAGTTAAAGGACCGCAGCGACTTCGTAATAGGAATTCGTGGAAGCTTCCTTAGGGATGATTATATTCTTGATGAAGAGCAGTTAGATTTGGTTGATGAGTTGGAAATTGCGAGCCTAGAACCATGGATTCGAGAATGTGGTTGCCTCCAAACTTTGCCAGAATGGTTAGTAAATCTCAAGTCTTTGCAAACATTGGATCTTACCTATGTCGTCCTTACCTAG

Protein sequence

MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHNSVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANRIKNITNQLQKHYDRAGHLGIVGSGSAGTETVISQIRQTVSELDDFGVVGRDVEVSSIVKQVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTIWNENSSLWDELKNCLMRMTEKSENSIVVTTRSVEVAKIMETLPSHHLSKLSVDQCWSLFQESANANGLPMTLSLEVVRESIPNVFGSCDENGDMHLKTVTISDCGVLGELPDGLKYCDSIQDLSIFRCSCLAVILERSGMVYSLTFVLPQKLKDRSDFVIGIRGSFLRDDYILDEEQLDLVDELEIASLEPWIRECGCLQTLPEWLVNLKSLQTLDLTYVVLT

Homology

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BLAST of MS027902 vs. NCBI nr
Match: XP_022150842.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 425.2 bits (1092), Expect = 6.8e-115
Identity = 234/364 (64.29%), Postives = 261/364 (71.70%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           MAE LLTFA E VL KVVKVA E IGLAWGLEK+LEEL++WLL  EAFL DINK K H +
Sbjct: 1   MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
           SV+LWVD+LQDLVHEAE+ LDELVYE LRRKVQT K   VR+F S SNN  VF VKMANR
Sbjct: 61  SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR 120

Query: 121 IKNITNQLQKHYDRAGHLGIVGSGSAGTETVISQIRQTVSELDDFGVVGRDVEVSSIVKQ 180
           +KNI++ L KHY  AG LG+V S SA TE  ISQ+R+TVSELDDF VVGRDVEVSSIV Q
Sbjct: 121 MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ 180

Query: 181 VVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTI---------------- 240
           V+DA N HVTSILPIVG+GGLGKTTLAKLVF+H RI++HFDKTI                
Sbjct: 181 VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGA 240

Query: 241 --------------------------------------WNENSSLWDELKNCLMRMTEKS 300
                                                 WNEN SLWDELKNCL R+TE+S
Sbjct: 241 ILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEES 300

Query: 301 ENSIVVTTRSVEVAKIMETLPSHHLSKLSVDQCWSLFQESANANGLPMTLSLEVVRESIP 311
           ENSI+VTTRS+EVAKIMETLPS+HLSKLS DQCW LFQESANANGLPMT SL+V +E + 
Sbjct: 301 ENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLV 360

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BLAST of MS027902 vs. NCBI nr
Match: XP_022158717.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 412.5 bits (1059), Expect = 4.5e-111
Identity = 224/364 (61.54%), Postives = 256/364 (70.33%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           MAE L TFAV+ VL KV KVA E IGLAWGL+KEL EL++WLL  E FL DINK K HH+
Sbjct: 1   MAEFLWTFAVQEVLKKVAKVAAEQIGLAWGLKKELHELREWLLKTETFLRDINKRKLHHD 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
           SV+LWVD+LQ LVHEAE+ LDELVYE LRRKV TGK   VRDF S SNN L F VKMA +
Sbjct: 61  SVRLWVDKLQHLVHEAEHLLDELVYEDLRRKVVTGKMKKVRDFVSPSNNILAFRVKMARK 120

Query: 121 IKNITNQLQKHYDRAGHLGIVGSGSAGTETVISQIRQTVSELDDFGVVGRDVEVSSIVKQ 180
           +KNIT+ L KHY  AG LG+VG GSA  E  I+Q+R+T+SELDDF VVGRDVEVS IV Q
Sbjct: 121 MKNITDLLCKHYCEAGPLGLVGDGSAEIEIDIAQLRETISELDDFEVVGRDVEVSKIVNQ 180

Query: 181 VVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTI---------------- 240
           ++DA N+HVTSILPIVG+GGLGKTT+AK+VFNH RIK HFDKTI                
Sbjct: 181 LIDATNTHVTSILPIVGMGGLGKTTMAKVVFNHERIKTHFDKTIWVCVSEPFLVNKILGA 240

Query: 241 --------------------------------------WNENSSLWDELKNCLMRMTEKS 300
                                                 WNE+ SLWDELKNCLMR+TE+S
Sbjct: 241 ILGTLRGTSSGLDNKEALLHELKKEMHGKRYFLVLDDVWNESHSLWDELKNCLMRITERS 300

Query: 301 ENSIVVTTRSVEVAKIMETLPSHHLSKLSVDQCWSLFQESANANGLPMTLSLEVVRESIP 311
           ENSI+VTTRS EVAKIMETLP+HHLSKLS DQCWSLF ESAN NGL +T SL+VV+E + 
Sbjct: 301 ENSILVTTRSAEVAKIMETLPNHHLSKLSDDQCWSLFLESANVNGLSITSSLKVVQELVK 360

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BLAST of MS027902 vs. NCBI nr
Match: XP_022944235.1 (disease resistance protein RGA2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 354.4 bits (908), Expect = 1.5e-93
Identity = 199/368 (54.08%), Postives = 240/368 (65.22%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           M E L TFAV+ +L KVV++A + IGL WGLEKEL  LK+WLL  E FL DIN  K H++
Sbjct: 1   MGEFLWTFAVQEMLKKVVRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHND 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQ-TGKRNNVRDFASSSNNPLVFPVKMAN 120
           SV+LWV++L  LVHEA++ +D LVYEHLR KVQ   K N V +F   SNN LVF  KMA 
Sbjct: 61  SVRLWVEKLHHLVHEADDLIDNLVYEHLRHKVQKNDKMNKVHNFLFPSNNSLVFRYKMAK 120

Query: 121 RIKNITNQLQKHYDRAGHLGIVGSGSAGT--ETVISQIRQTVSELDDFGVVGRDVEVSSI 180
           ++ N  NQL KH   A  LG+VG     T  ++ +SQIR+TVSE+DDF VVGRDVEVSSI
Sbjct: 121 KMNNFMNQLNKHDREATRLGLVGDEPIQTLLQSDVSQIRETVSEVDDFEVVGRDVEVSSI 180

Query: 181 VKQVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTI------------- 240
           VKQV+DA N  VTSILPIVG+GGLGKTTLA+L+FNH  IKQHFD+TI             
Sbjct: 181 VKQVIDAHNQRVTSILPIVGMGGLGKTTLARLIFNHEMIKQHFDETIWVCVSIPFDINKI 240

Query: 241 -----------------------------------------WNENSSLWDELKNCLMRMT 300
                                                    WNE+S LW++LKN LM++T
Sbjct: 241 LGAILQNVKGHDGCYHNREILLRELKKEMYGKRYFLVLDDVWNEDSLLWEQLKNFLMKIT 300

Query: 301 EKSENSIVVTTRSVEVAKIME-TLPSHHLSKLSVDQCWSLFQESANANGLPMTLSLEVVR 311
            KS NSI+VTTR+VEV KIME  LP+HH++KLS +QCWSLF ESANANGLP T +LE VR
Sbjct: 301 SKSGNSILVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVR 360

[Close]

BLAST of MS027902 vs. NCBI nr
Match: KAA0042414.1 (disease resistance protein RGA2-like [Cucumis melo var. makuwa] >TYK00998.1 disease resistance protein RGA2-like [Cucumis melo var. makuwa])

HSP 1 Score: 340.9 bits (873), Expect = 1.7e-89
Identity = 188/366 (51.37%), Postives = 237/366 (64.75%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           M + L TFAVE +L KV+KVA E  GLAWG +K L +L+KWLL  EAFL DIN  K HH+
Sbjct: 1   MGDFLWTFAVEEMLKKVLKVAQEQTGLAWGFQKHLSKLQKWLLKAEAFLRDINTRKLHHD 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
           SV++WVD+L+ LV++A++ LDE+VYEHLR+KV T K   V DF S S+N  +F   MAN+
Sbjct: 61  SVRMWVDDLRHLVYQADDLLDEIVYEHLRQKVHTRKMKKVCDFFSPSSNAFIFRRNMANK 120

Query: 121 IKNITNQLQKHYDRAGHLGIVGSGSAGTE-TVISQIRQTVSELDDFGVVGRDVEVSSIVK 180
           +  +   L+KHY+ A  LG+V + +A  E  VISQ R+T+SEL+D  +VGRDVEV SIVK
Sbjct: 121 MMTLVELLEKHYNEAAPLGLVVNENARPEIDVISQYRETISELEDHKIVGRDVEVESIVK 180

Query: 181 QVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKT---------------- 240
           QV+DA N+ +TSILPIVG+GGLGKTTLAKLVFNH  ++QHFDKT                
Sbjct: 181 QVIDASNNQLTSILPIVGMGGLGKTTLAKLVFNHELVRQHFDKTVWVCVSEPFIVNKILL 240

Query: 241 ---------------------------------------IWNENSSLWDELKNCLMRMTE 300
                                                  +WNENS LWDELK CL+++T 
Sbjct: 241 DILQNLQGGISNGGDSKEVLLRELQKEMLGQTYFLVLDDVWNENSFLWDELKYCLLKITG 300

Query: 301 KSENSIVVTTRSVEVAKIMETLPSHHLSKLSVDQCWSLFQESANANGLPMTLSLEVVRES 311
            S+NSIVVTTRSVEVAKIM T   H LSKLS D CWSLF+ESANA GL MT +L ++++ 
Sbjct: 301 NSKNSIVVTTRSVEVAKIMGTCSGHLLSKLSDDYCWSLFKESANAYGLSMTSNLGIIQKE 360

[Close]

BLAST of MS027902 vs. NCBI nr
Match: XP_008462780.1 (PREDICTED: disease resistance protein RGA2-like [Cucumis melo])

HSP 1 Score: 340.9 bits (873), Expect = 1.7e-89
Identity = 188/366 (51.37%), Postives = 237/366 (64.75%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           M + L TFAVE +L KV+KVA E  GLAWG +K L +L+KWLL  EAFL DIN  K HH+
Sbjct: 1   MGDFLWTFAVEEMLKKVLKVAQEQTGLAWGFQKHLSKLQKWLLKAEAFLRDINTRKLHHD 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
           SV++WVD+L+ LV++A++ LDE+VYEHLR+KV T K   V DF S S+N  +F   MAN+
Sbjct: 61  SVRMWVDDLRHLVYQADDLLDEIVYEHLRQKVHTRKMKKVCDFFSPSSNAFIFRRNMANK 120

Query: 121 IKNITNQLQKHYDRAGHLGIVGSGSAGTE-TVISQIRQTVSELDDFGVVGRDVEVSSIVK 180
           +  +   L+KHY+ A  LG+V + +A  E  VISQ R+T+SEL+D  +VGRDVEV SIVK
Sbjct: 121 MMTLVELLEKHYNEAAPLGLVVNENARPEIDVISQYRETISELEDHKIVGRDVEVESIVK 180

Query: 181 QVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKT---------------- 240
           QV+DA N+ +TSILPIVG+GGLGKTTLAKLVFNH  ++QHFDKT                
Sbjct: 181 QVIDASNNQLTSILPIVGMGGLGKTTLAKLVFNHELVRQHFDKTVWVCVSEPFIVNKILL 240

Query: 241 ---------------------------------------IWNENSSLWDELKNCLMRMTE 300
                                                  +WNENS LWDELK CL+++T 
Sbjct: 241 DILQNLQGGISNGGDSKEVLLRELQKEMLGQTYFLVLDDVWNENSFLWDELKYCLLKITG 300

Query: 301 KSENSIVVTTRSVEVAKIMETLPSHHLSKLSVDQCWSLFQESANANGLPMTLSLEVVRES 311
            S+NSIVVTTRSVEVAKIM T   H LSKLS D CWSLF+ESANA GL MT +L ++++ 
Sbjct: 301 NSKNSIVVTTRSVEVAKIMGTCSGHLLSKLSDDYCWSLFKESANAYGLSMTSNLGIIQKE 360

[Close]

BLAST of MS027902 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 102.8 bits (255), Expect = 1.0e-20
Identity = 92/340 (27.06%), Postives = 143/340 (42.06%), Query Frame = 0


Query: 6   LTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHNSVKLW 65
           LTF ++G L           GL +G EKE ++L      I+A L D  + +  + ++K W
Sbjct: 13  LTFFIQGEL-----------GLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNW 72

Query: 66  VDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANRIKNIT 125
           + +L    +E ++ LD+   E  R K     R + R         + F  K+  R+K + 
Sbjct: 73  LQKLNVAAYEVDDILDDCKTEAARFKQAVLGRYHPR--------TITFCYKVGKRMKEMM 132

Query: 126 NQLQKHYDRAGHLGIVGSGSAGTETVISQ---IRQTVSELDDFGVVGRDVEVSSIVKQVV 185
            +L    +   +  +        E +I +    RQT   L +  V GR+ E   IVK ++
Sbjct: 133 EKLDAIAEERRNFHL-------DERIIERQAARRQTGFVLTEPKVYGREKEEDEIVKILI 192

Query: 186 -DARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFD-------------------- 245
            +   S    +LPI+G+GGLGKTTLA++VFN  RI +HF+                    
Sbjct: 193 NNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAI 252

Query: 246 ----------------------------------KTIWNENSSLWDELKNCLMRMTEKSE 288
                                               +WNE+   WD L+  L      S 
Sbjct: 253 VESIEGKSLGDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVL--KIGASG 312

[Close]

BLAST of MS027902 vs. ExPASy Swiss-Prot
Match: Q9FJB5 (Disease resistance RPP8-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RPP8L3 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.0e-17
Identity = 67/230 (29.13%), Postives = 125/230 (54.35%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           MAE +++F V+ + + + + +  L     G++++++ LK+ L  +++ L D +  K   +
Sbjct: 1   MAEGVVSFGVQKLWALLNRESERLN----GIDEQVDGLKRQLRGLQSLLKDADAKKHGSD 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
            V+ ++++++DLV +AE+ ++  V   LR + + G +N+VR  A      L    K+A+ 
Sbjct: 61  RVRNFLEDVKDLVFDAEDIIESYVLNKLRGEGK-GVKNHVRRLACF----LTDRHKVASD 120

Query: 121 IKNITNQLQKHYDRAGHLGIV-----GSGSAGTETVISQIRQTVSELDDFGVVGRDVEVS 180
           I+ IT ++ K       LGI      G  S   + +  +IRQT     +  +VG +  V 
Sbjct: 121 IEGITKRISKVIGEMQSLGIQQQIIDGGRSLSLQDIQREIRQTFPNSSESDLVGVEQSVE 180

Query: 181 SIVKQVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTIW 226
            +V  +V+  N  V S   I G+GG+GKTTLA+ +F+H  +++HFD   W
Sbjct: 181 ELVGPMVEIDNIQVVS---ISGMGGIGKTTLARQIFHHDLVRRHFDGFAW 218

[Close]

BLAST of MS027902 vs. ExPASy Swiss-Prot
Match: P0C8S1 (Probable disease resistance RPP8-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RPP8L2 PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.6e-16
Identity = 66/230 (28.70%), Postives = 122/230 (53.04%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           MAE +++F VE +   + + +  L     G++++++ LK+ L  +++ L D +  K    
Sbjct: 1   MAEAVVSFGVEKLWELLSRESARLN----GIDEQVDGLKRQLGRLQSLLKDADAKKNETE 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
            V+ ++++++D+V++A++ ++  +   LR K + G +  VR  A      LV   K A+ 
Sbjct: 61  RVRNFLEDVKDIVYDADDIIESFLLNELRGK-EKGIKKQVRTLACF----LVDRRKFASD 120

Query: 121 IKNITNQLQKHYDRAGHLGIVGSGSAGTETVISQ-----IRQTVSELDDFGVVGRDVEVS 180
           I+ IT ++ +       LGI      G  ++  Q     IRQT S   +  +VG D  V 
Sbjct: 121 IEGITKRISEVIVGMQSLGIQHIADGGGRSLSLQERQREIRQTFSRNSESDLVGLDQSVE 180

Query: 181 SIVKQVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTIW 226
            +V  +V+  +  V S   + G+GG+GKTTLA+ VF+H  +++HFD   W
Sbjct: 181 ELVDHLVENDSVQVVS---VSGMGGIGKTTLARQVFHHDIVRRHFDGFSW 218

[Close]

BLAST of MS027902 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.3e-15
Identity = 72/264 (27.27%), Postives = 125/264 (47.35%), Query Frame = 0


Query: 32  EKELEELKKWLLNIEAFLCDINKSKFHHNSVKLWVDELQDLVHEAENPLDELVYEHLRRK 91
           E  LE L   LL I A L D  + +  +  V+ WV+EL+D+V+ AE+ LD++  E LR  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 92  V-----------QTGKRNNVRDFASSSNNPLVFPVKMANRIKNITNQLQKHYDRAGHLGI 151
           +           Q   R ++ DF   ++  L        R++ +T +L++   +   LG+
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHL------ETRLEKVTIRLERLASQRNILGL 155

Query: 152 VGSGSAGTETVISQIRQTVSELDDFGVVGRDVEVSSIVKQVV--DARNSHVTSILPIVGV 211
                  T  +  Q   T S +D+  V GRD +   I++ ++  + +++ +T ++ IVG+
Sbjct: 156 ----KELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGIT-VVAIVGI 215

Query: 212 GGLGKTTLAKLVFNHPRIKQHFDKTIWNENSSLWDELKNCLMRMTEKSENSIVVTTRSVE 271
           GG+GKTTL++L++N   ++ +F   +W   S  +D  K         +      T   V 
Sbjct: 216 GGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVL 275

Query: 272 VAKIMETLPSHHLSKLSV-DQCWS 282
             K+ E L    L  L V D  W+
Sbjct: 276 QVKLKERLTGTGLPFLLVLDDLWN 288

[Close]

BLAST of MS027902 vs. ExPASy Swiss-Prot
Match: F4IBE4 (Probable disease resistance protein RF45 OS=Arabidopsis thaliana OX=3702 GN=RF45 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 4.8e-15
Identity = 77/279 (27.60%), Postives = 134/279 (48.03%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           MA  L++F ++ + +    + ++   L  G+E ++ ELK+ L  + +FL D N  K    
Sbjct: 1   MAGELISFGIQNLWN----LLSQECELFQGVEDQVTELKRDLNMLSSFLKDANAKKHTSA 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPV---KM 120
            VK  V+E+++++++ E+ ++  V E    K  +G + ++R  A    +   + +    +
Sbjct: 61  VVKNCVEEIKEIIYDGEDTIETFVLEQNLGKT-SGIKKSIRRLACIIPDRRRYALGIGGL 120

Query: 121 ANRIKNITNQLQKHYDRAGHLGIVGSGSAGTE-TVISQIRQTVSELDDFGVVGRDVEVSS 180
           +NRI  +   +Q          IV  G    +     ++RQ  S+ DD   VG +  V  
Sbjct: 121 SNRISKVIRDMQSF---GVQQAIVDGGYKQPQGDKQREMRQKFSKDDDSDFVGLEANVKK 180

Query: 181 IVKQVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTIW------NENSS 240
           +V  +VD  N  V S   I G+GGLGKTTLAK VFNH  +K  FD   W          +
Sbjct: 181 LVGYLVDEANVQVVS---ITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMN 240

Query: 241 LWDE-LKNCLMRMTEKSENSIVVTTRSVEVAKIMETLPS 269
           +W + L++   +  EK    +   T   E+ +++ET  S
Sbjct: 241 VWQKILRDLKPKEEEKKIMEMTQDTLQGELIRLLETSKS 268

[Close]

BLAST of MS027902 vs. ExPASy TrEMBL
Match: A0A6J1DBV6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111018890 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 3.3e-115
Identity = 234/364 (64.29%), Postives = 261/364 (71.70%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           MAE LLTFA E VL KVVKVA E IGLAWGLEK+LEEL++WLL  EAFL DINK K H +
Sbjct: 1   MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
           SV+LWVD+LQDLVHEAE+ LDELVYE LRRKVQT K   VR+F S SNN  VF VKMANR
Sbjct: 61  SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR 120

Query: 121 IKNITNQLQKHYDRAGHLGIVGSGSAGTETVISQIRQTVSELDDFGVVGRDVEVSSIVKQ 180
           +KNI++ L KHY  AG LG+V S SA TE  ISQ+R+TVSELDDF VVGRDVEVSSIV Q
Sbjct: 121 MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ 180

Query: 181 VVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTI---------------- 240
           V+DA N HVTSILPIVG+GGLGKTTLAKLVF+H RI++HFDKTI                
Sbjct: 181 VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGA 240

Query: 241 --------------------------------------WNENSSLWDELKNCLMRMTEKS 300
                                                 WNEN SLWDELKNCL R+TE+S
Sbjct: 241 ILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEES 300

Query: 301 ENSIVVTTRSVEVAKIMETLPSHHLSKLSVDQCWSLFQESANANGLPMTLSLEVVRESIP 311
           ENSI+VTTRS+EVAKIMETLPS+HLSKLS DQCW LFQESANANGLPMT SL+V +E + 
Sbjct: 301 ENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLV 360

[Close]

BLAST of MS027902 vs. ExPASy TrEMBL
Match: A0A6J1DXY1 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111025175 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 2.2e-111
Identity = 224/364 (61.54%), Postives = 256/364 (70.33%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           MAE L TFAV+ VL KV KVA E IGLAWGL+KEL EL++WLL  E FL DINK K HH+
Sbjct: 1   MAEFLWTFAVQEVLKKVAKVAAEQIGLAWGLKKELHELREWLLKTETFLRDINKRKLHHD 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
           SV+LWVD+LQ LVHEAE+ LDELVYE LRRKV TGK   VRDF S SNN L F VKMA +
Sbjct: 61  SVRLWVDKLQHLVHEAEHLLDELVYEDLRRKVVTGKMKKVRDFVSPSNNILAFRVKMARK 120

Query: 121 IKNITNQLQKHYDRAGHLGIVGSGSAGTETVISQIRQTVSELDDFGVVGRDVEVSSIVKQ 180
           +KNIT+ L KHY  AG LG+VG GSA  E  I+Q+R+T+SELDDF VVGRDVEVS IV Q
Sbjct: 121 MKNITDLLCKHYCEAGPLGLVGDGSAEIEIDIAQLRETISELDDFEVVGRDVEVSKIVNQ 180

Query: 181 VVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTI---------------- 240
           ++DA N+HVTSILPIVG+GGLGKTT+AK+VFNH RIK HFDKTI                
Sbjct: 181 LIDATNTHVTSILPIVGMGGLGKTTMAKVVFNHERIKTHFDKTIWVCVSEPFLVNKILGA 240

Query: 241 --------------------------------------WNENSSLWDELKNCLMRMTEKS 300
                                                 WNE+ SLWDELKNCLMR+TE+S
Sbjct: 241 ILGTLRGTSSGLDNKEALLHELKKEMHGKRYFLVLDDVWNESHSLWDELKNCLMRITERS 300

Query: 301 ENSIVVTTRSVEVAKIMETLPSHHLSKLSVDQCWSLFQESANANGLPMTLSLEVVRESIP 311
           ENSI+VTTRS EVAKIMETLP+HHLSKLS DQCWSLF ESAN NGL +T SL+VV+E + 
Sbjct: 301 ENSILVTTRSAEVAKIMETLPNHHLSKLSDDQCWSLFLESANVNGLSITSSLKVVQELVK 360

[Close]

BLAST of MS027902 vs. ExPASy TrEMBL
Match: A0A6J1FYJ5 (disease resistance protein RGA2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448744 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 7.1e-94
Identity = 199/368 (54.08%), Postives = 240/368 (65.22%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           M E L TFAV+ +L KVV++A + IGL WGLEKEL  LK+WLL  E FL DIN  K H++
Sbjct: 1   MGEFLWTFAVQEMLKKVVRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHND 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQ-TGKRNNVRDFASSSNNPLVFPVKMAN 120
           SV+LWV++L  LVHEA++ +D LVYEHLR KVQ   K N V +F   SNN LVF  KMA 
Sbjct: 61  SVRLWVEKLHHLVHEADDLIDNLVYEHLRHKVQKNDKMNKVHNFLFPSNNSLVFRYKMAK 120

Query: 121 RIKNITNQLQKHYDRAGHLGIVGSGSAGT--ETVISQIRQTVSELDDFGVVGRDVEVSSI 180
           ++ N  NQL KH   A  LG+VG     T  ++ +SQIR+TVSE+DDF VVGRDVEVSSI
Sbjct: 121 KMNNFMNQLNKHDREATRLGLVGDEPIQTLLQSDVSQIRETVSEVDDFEVVGRDVEVSSI 180

Query: 181 VKQVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTI------------- 240
           VKQV+DA N  VTSILPIVG+GGLGKTTLA+L+FNH  IKQHFD+TI             
Sbjct: 181 VKQVIDAHNQRVTSILPIVGMGGLGKTTLARLIFNHEMIKQHFDETIWVCVSIPFDINKI 240

Query: 241 -----------------------------------------WNENSSLWDELKNCLMRMT 300
                                                    WNE+S LW++LKN LM++T
Sbjct: 241 LGAILQNVKGHDGCYHNREILLRELKKEMYGKRYFLVLDDVWNEDSLLWEQLKNFLMKIT 300

Query: 301 EKSENSIVVTTRSVEVAKIME-TLPSHHLSKLSVDQCWSLFQESANANGLPMTLSLEVVR 311
            KS NSI+VTTR+VEV KIME  LP+HH++KLS +QCWSLF ESANANGLP T +LE VR
Sbjct: 301 SKSGNSILVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVR 360

[Close]

BLAST of MS027902 vs. ExPASy TrEMBL
Match: A0A5A7TG70 (Disease resistance protein RGA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001980 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 8.1e-90
Identity = 188/366 (51.37%), Postives = 237/366 (64.75%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           M + L TFAVE +L KV+KVA E  GLAWG +K L +L+KWLL  EAFL DIN  K HH+
Sbjct: 1   MGDFLWTFAVEEMLKKVLKVAQEQTGLAWGFQKHLSKLQKWLLKAEAFLRDINTRKLHHD 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
           SV++WVD+L+ LV++A++ LDE+VYEHLR+KV T K   V DF S S+N  +F   MAN+
Sbjct: 61  SVRMWVDDLRHLVYQADDLLDEIVYEHLRQKVHTRKMKKVCDFFSPSSNAFIFRRNMANK 120

Query: 121 IKNITNQLQKHYDRAGHLGIVGSGSAGTE-TVISQIRQTVSELDDFGVVGRDVEVSSIVK 180
           +  +   L+KHY+ A  LG+V + +A  E  VISQ R+T+SEL+D  +VGRDVEV SIVK
Sbjct: 121 MMTLVELLEKHYNEAAPLGLVVNENARPEIDVISQYRETISELEDHKIVGRDVEVESIVK 180

Query: 181 QVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKT---------------- 240
           QV+DA N+ +TSILPIVG+GGLGKTTLAKLVFNH  ++QHFDKT                
Sbjct: 181 QVIDASNNQLTSILPIVGMGGLGKTTLAKLVFNHELVRQHFDKTVWVCVSEPFIVNKILL 240

Query: 241 ---------------------------------------IWNENSSLWDELKNCLMRMTE 300
                                                  +WNENS LWDELK CL+++T 
Sbjct: 241 DILQNLQGGISNGGDSKEVLLRELQKEMLGQTYFLVLDDVWNENSFLWDELKYCLLKITG 300

Query: 301 KSENSIVVTTRSVEVAKIMETLPSHHLSKLSVDQCWSLFQESANANGLPMTLSLEVVRES 311
            S+NSIVVTTRSVEVAKIM T   H LSKLS D CWSLF+ESANA GL MT +L ++++ 
Sbjct: 301 NSKNSIVVTTRSVEVAKIMGTCSGHLLSKLSDDYCWSLFKESANAYGLSMTSNLGIIQKE 360

[Close]

BLAST of MS027902 vs. ExPASy TrEMBL
Match: A0A1S3CHQ5 (disease resistance protein RGA2-like OS=Cucumis melo OX=3656 GN=LOC103501070 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 8.1e-90
Identity = 188/366 (51.37%), Postives = 237/366 (64.75%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           M + L TFAVE +L KV+KVA E  GLAWG +K L +L+KWLL  EAFL DIN  K HH+
Sbjct: 1   MGDFLWTFAVEEMLKKVLKVAQEQTGLAWGFQKHLSKLQKWLLKAEAFLRDINTRKLHHD 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
           SV++WVD+L+ LV++A++ LDE+VYEHLR+KV T K   V DF S S+N  +F   MAN+
Sbjct: 61  SVRMWVDDLRHLVYQADDLLDEIVYEHLRQKVHTRKMKKVCDFFSPSSNAFIFRRNMANK 120

Query: 121 IKNITNQLQKHYDRAGHLGIVGSGSAGTE-TVISQIRQTVSELDDFGVVGRDVEVSSIVK 180
           +  +   L+KHY+ A  LG+V + +A  E  VISQ R+T+SEL+D  +VGRDVEV SIVK
Sbjct: 121 MMTLVELLEKHYNEAAPLGLVVNENARPEIDVISQYRETISELEDHKIVGRDVEVESIVK 180

Query: 181 QVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKT---------------- 240
           QV+DA N+ +TSILPIVG+GGLGKTTLAKLVFNH  ++QHFDKT                
Sbjct: 181 QVIDASNNQLTSILPIVGMGGLGKTTLAKLVFNHELVRQHFDKTVWVCVSEPFIVNKILL 240

Query: 241 ---------------------------------------IWNENSSLWDELKNCLMRMTE 300
                                                  +WNENS LWDELK CL+++T 
Sbjct: 241 DILQNLQGGISNGGDSKEVLLRELQKEMLGQTYFLVLDDVWNENSFLWDELKYCLLKITG 300

Query: 301 KSENSIVVTTRSVEVAKIMETLPSHHLSKLSVDQCWSLFQESANANGLPMTLSLEVVRES 311
            S+NSIVVTTRSVEVAKIM T   H LSKLS D CWSLF+ESANA GL MT +L ++++ 
Sbjct: 301 NSKNSIVVTTRSVEVAKIMGTCSGHLLSKLSDDYCWSLFKESANAYGLSMTSNLGIIQKE 360

[Close]

BLAST of MS027902 vs. TAIR 10
Match: AT5G35450.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 90.9 bits (224), Expect = 2.8e-18
Identity = 67/230 (29.13%), Postives = 125/230 (54.35%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           MAE +++F V+ + + + + +  L     G++++++ LK+ L  +++ L D +  K   +
Sbjct: 1   MAEGVVSFGVQKLWALLNRESERLN----GIDEQVDGLKRQLRGLQSLLKDADAKKHGSD 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
            V+ ++++++DLV +AE+ ++  V   LR + + G +N+VR  A      L    K+A+ 
Sbjct: 61  RVRNFLEDVKDLVFDAEDIIESYVLNKLRGEGK-GVKNHVRRLACF----LTDRHKVASD 120

Query: 121 IKNITNQLQKHYDRAGHLGIV-----GSGSAGTETVISQIRQTVSELDDFGVVGRDVEVS 180
           I+ IT ++ K       LGI      G  S   + +  +IRQT     +  +VG +  V 
Sbjct: 121 IEGITKRISKVIGEMQSLGIQQQIIDGGRSLSLQDIQREIRQTFPNSSESDLVGVEQSVE 180

Query: 181 SIVKQVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTIW 226
            +V  +V+  N  V S   I G+GG+GKTTLA+ +F+H  +++HFD   W
Sbjct: 181 ELVGPMVEIDNIQVVS---ISGMGGIGKTTLARQIFHHDLVRRHFDGFAW 218

[Close]

BLAST of MS027902 vs. TAIR 10
Match: AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 88.2 bits (217), Expect = 1.8e-17
Identity = 66/230 (28.70%), Postives = 122/230 (53.04%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           MAE +++F VE +   + + +  L     G++++++ LK+ L  +++ L D +  K    
Sbjct: 1   MAEAVVSFGVEKLWELLSRESARLN----GIDEQVDGLKRQLGRLQSLLKDADAKKNETE 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPVKMANR 120
            V+ ++++++D+V++A++ ++  +   LR K + G +  VR  A      LV   K A+ 
Sbjct: 61  RVRNFLEDVKDIVYDADDIIESFLLNELRGK-EKGIKKQVRTLACF----LVDRRKFASD 120

Query: 121 IKNITNQLQKHYDRAGHLGIVGSGSAGTETVISQ-----IRQTVSELDDFGVVGRDVEVS 180
           I+ IT ++ +       LGI      G  ++  Q     IRQT S   +  +VG D  V 
Sbjct: 121 IEGITKRISEVIVGMQSLGIQHIADGGGRSLSLQERQREIRQTFSRNSESDLVGLDQSVE 180

Query: 181 SIVKQVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTIW 226
            +V  +V+  +  V S   + G+GG+GKTTLA+ VF+H  +++HFD   W
Sbjct: 181 ELVDHLVENDSVQVVS---VSGMGGIGKTTLARQVFHHDIVRRHFDGFSW 218

[Close]

BLAST of MS027902 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 85.9 bits (211), Expect = 9.1e-17
Identity = 72/264 (27.27%), Postives = 125/264 (47.35%), Query Frame = 0


Query: 32  EKELEELKKWLLNIEAFLCDINKSKFHHNSVKLWVDELQDLVHEAENPLDELVYEHLRRK 91
           E  LE L   LL I A L D  + +  +  V+ WV+EL+D+V+ AE+ LD++  E LR  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 92  V-----------QTGKRNNVRDFASSSNNPLVFPVKMANRIKNITNQLQKHYDRAGHLGI 151
           +           Q   R ++ DF   ++  L        R++ +T +L++   +   LG+
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHL------ETRLEKVTIRLERLASQRNILGL 155

Query: 152 VGSGSAGTETVISQIRQTVSELDDFGVVGRDVEVSSIVKQVV--DARNSHVTSILPIVGV 211
                  T  +  Q   T S +D+  V GRD +   I++ ++  + +++ +T ++ IVG+
Sbjct: 156 ----KELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGIT-VVAIVGI 215

Query: 212 GGLGKTTLAKLVFNHPRIKQHFDKTIWNENSSLWDELKNCLMRMTEKSENSIVVTTRSVE 271
           GG+GKTTL++L++N   ++ +F   +W   S  +D  K         +      T   V 
Sbjct: 216 GGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVL 275

Query: 272 VAKIMETLPSHHLSKLSV-DQCWS 282
             K+ E L    L  L V D  W+
Sbjct: 276 QVKLKERLTGTGLPFLLVLDDLWN 288

[Close]

BLAST of MS027902 vs. TAIR 10
Match: AT1G58807.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 84.0 bits (206), Expect = 3.4e-16
Identity = 77/279 (27.60%), Postives = 134/279 (48.03%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           MA  L++F ++ + +    + ++   L  G+E ++ ELK+ L  + +FL D N  K    
Sbjct: 1   MAGELISFGIQNLWN----LLSQECELFQGVEDQVTELKRDLNMLSSFLKDANAKKHTSA 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPV---KM 120
            VK  V+E+++++++ E+ ++  V E    K  +G + ++R  A    +   + +    +
Sbjct: 61  VVKNCVEEIKEIIYDGEDTIETFVLEQNLGKT-SGIKKSIRRLACIIPDRRRYALGIGGL 120

Query: 121 ANRIKNITNQLQKHYDRAGHLGIVGSGSAGTE-TVISQIRQTVSELDDFGVVGRDVEVSS 180
           +NRI  +   +Q          IV  G    +     ++RQ  S+ DD   VG +  V  
Sbjct: 121 SNRISKVIRDMQSF---GVQQAIVDGGYKQPQGDKQREMRQKFSKDDDSDFVGLEANVKK 180

Query: 181 IVKQVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTIW------NENSS 240
           +V  +VD  N  V S   I G+GGLGKTTLAK VFNH  +K  FD   W          +
Sbjct: 181 LVGYLVDEANVQVVS---ITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMN 240

Query: 241 LWDE-LKNCLMRMTEKSENSIVVTTRSVEVAKIMETLPS 269
           +W + L++   +  EK    +   T   E+ +++ET  S
Sbjct: 241 VWQKILRDLKPKEEEKKIMEMTQDTLQGELIRLLETSKS 268

[Close]

BLAST of MS027902 vs. TAIR 10
Match: AT1G59124.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 84.0 bits (206), Expect = 3.4e-16
Identity = 77/279 (27.60%), Postives = 134/279 (48.03%), Query Frame = 0


Query: 1   MAELLLTFAVEGVLSKVVKVATELIGLAWGLEKELEELKKWLLNIEAFLCDINKSKFHHN 60
           MA  L++F ++ + +    + ++   L  G+E ++ ELK+ L  + +FL D N  K    
Sbjct: 1   MAGELISFGIQNLWN----LLSQECELFQGVEDQVTELKRDLNMLSSFLKDANAKKHTSA 60

Query: 61  SVKLWVDELQDLVHEAENPLDELVYEHLRRKVQTGKRNNVRDFASSSNNPLVFPV---KM 120
            VK  V+E+++++++ E+ ++  V E    K  +G + ++R  A    +   + +    +
Sbjct: 61  VVKNCVEEIKEIIYDGEDTIETFVLEQNLGKT-SGIKKSIRRLACIIPDRRRYALGIGGL 120

Query: 121 ANRIKNITNQLQKHYDRAGHLGIVGSGSAGTE-TVISQIRQTVSELDDFGVVGRDVEVSS 180
           +NRI  +   +Q          IV  G    +     ++RQ  S+ DD   VG +  V  
Sbjct: 121 SNRISKVIRDMQSF---GVQQAIVDGGYKQPQGDKQREMRQKFSKDDDSDFVGLEANVKK 180

Query: 181 IVKQVVDARNSHVTSILPIVGVGGLGKTTLAKLVFNHPRIKQHFDKTIW------NENSS 240
           +V  +VD  N  V S   I G+GGLGKTTLAK VFNH  +K  FD   W          +
Sbjct: 181 LVGYLVDEANVQVVS---ITGMGGLGKTTLAKQVFNHEDVKHQFDGLSWVCVSQDFTRMN 240

Query: 241 LWDE-LKNCLMRMTEKSENSIVVTTRSVEVAKIMETLPS 269
           +W + L++   +  EK    +   T   E+ +++ET  S
Sbjct: 241 VWQKILRDLKPKEEEKKIMEMTQDTLQGELIRLLETSKS 268

The following BLAST results are available for this feature:

BLAST of MS027902 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150842.1	6.8e-115	64.29	putative disease resistance protein RGA3 [Momordica charantia]	[more]
XP_022158717.1	4.5e-111	61.54	putative disease resistance protein RGA3 [Momordica charantia]	[more]
XP_022944235.1	1.5e-93	54.08	disease resistance protein RGA2-like isoform X2 [Cucurbita moschata]	[more]
KAA0042414.1	1.7e-89	51.37	disease resistance protein RGA2-like [Cucumis melo var. makuwa] >TYK00998.1 dise...	[more]
XP_008462780.1	1.7e-89	51.37	PREDICTED: disease resistance protein RGA2-like [Cucumis melo]	[more]

BLAST of MS027902 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XA40	1.0e-20	27.06	Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q9FJB5	4.0e-17	29.13	Disease resistance RPP8-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RPP8L3...	[more]
P0C8S1	2.6e-16	28.70	Probable disease resistance RPP8-like protein 2 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9LRR4	1.3e-15	27.27	Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702...	[more]
F4IBE4	4.8e-15	27.60	Probable disease resistance protein RF45 OS=Arabidopsis thaliana OX=3702 GN=RF45...	[more]

BLAST of MS027902 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBV6	3.3e-115	64.29	putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1DXY1	2.2e-111	61.54	putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1FYJ5	7.1e-94	54.08	disease resistance protein RGA2-like isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A5A7TG70	8.1e-90	51.37	Disease resistance protein RGA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3CHQ5	8.1e-90	51.37	disease resistance protein RGA2-like OS=Cucumis melo OX=3656 GN=LOC103501070 PE=...	[more]

BLAST of MS027902 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35450.1	2.8e-18	29.13	Disease resistance protein (CC-NBS-LRR class) family	[more]
AT1G53350.1	1.8e-17	28.70	Disease resistance protein (CC-NBS-LRR class) family	[more]
AT3G14470.1	9.1e-17	27.27	NB-ARC domain-containing disease resistance protein	[more]
AT1G58807.1	3.4e-16	27.60	Disease resistance protein (CC-NBS-LRR class) family	[more]
AT1G59124.1	3.4e-16	27.60	Disease resistance protein (CC-NBS-LRR class) family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00364	DISEASERSIST	coord: 192..207 score: 52.03 coord: 431..447 score: 33.09
None	No IPR available	GENE3D	1.20.5.4130		coord: 8..124 e-value: 3.0E-17 score: 64.7
None	No IPR available	PANTHER	PTHR23155:SF1132	OS08G0543100 PROTEIN	coord: 224..288 coord: 27..216
None	No IPR available	SUPERFAMILY	52047	RNI-like	coord: 269..443
IPR002182	NB-ARC	PFAM	PF00931	NB-ARC	coord: 224..299 e-value: 3.7E-8 score: 32.9
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 254..447 e-value: 6.4E-6 score: 26.6
IPR041118	Rx, N-terminal	PFAM	PF18052	Rx_N	coord: 9..97 e-value: 8.2E-18 score: 64.5
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 149..249 e-value: 7.9E-16 score: 60.0
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 151..297
IPR044974	Disease resistance protein, plants	PANTHER	PTHR23155	DISEASE RESISTANCE PROTEIN RP	coord: 27..216
IPR044974	Disease resistance protein, plants	PANTHER	PTHR23155	DISEASE RESISTANCE PROTEIN RP	coord: 224..288

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS027902.1	MS027902.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
molecular_function	GO:0043531	ADP binding