MS024344 (gene) Bitter gourd (TR) v1

Overview
NameMS024344
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionE3 ubiquitin-protein ligase PRT1 isoform X1
Locationscaffold318: 233109 .. 239721 (-)
RNA-Seq ExpressionMS024344
SyntenyMS024344
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCTACTTCTCTCCGCAATTTGATGCCCTTGCATGTGACTCACAAGCTGGTAAAAAAGTGGAACATTCAGAAGATTCTGCTAATGGCGAGCTGAAGACAAATACCAATAATGACAAAGTTGTTGCTGAACGCATATTAGAAGAAAATTCTGCAGTTGTCAGTCCTTATGGTCAAAAGACGCAAAACCATGAGGAGGTTTCAGTTGCTGACTTGCTATGTCGTGCATGCACCCAATTGCTCTTCCGACCTGTGGTTCTTAATTGTGGCCATGGTATATATAATCTCTCTCTATCTCTGTGTCGGGTTGCATGCTATTTTATCCTCAACTATAATGAAAGTTAATCTATAAGTTTGGTTTTCGAATTTTCAGAGTTGGGTCTATTTAGTCCTTTTATTTGATAGGCCCTTGAACTTTTAAGGTTGTGTATATCAGAACTTTGTACGTTAATTTTGAACTATCAAATTTGCCTGGCCATTACTTTAACATTTAAATGACAGGGTTGCTATCTATCTATATCTATAATGTACTAACAAGCTACTATCTTTGAAAAAAAATTATTTGGACCATTTTGTCCTCCTATAAAATTTTGTGTTCACAAAATATGGCACTTACAATTAATTACCAATTATTGGTAAATTATAAATTTAATTGTTAGAATTTGAAAAGTTTTTTCTTTATCTTTTTTTTTTTCTTTAAAATTTATAAGAAAGATAAAAAAAAGTATATTGAAAGAGGGAAATCACCCAAAAAGAGACTGCCTAAAGGCAGAGGGTAGAGAAAACCCTCCCCTAAGAACTATACAATCAAAGCTTTCCAATTTTGTAGAATAAGTTGGAGGCTATAATTACAAAAAAACTTCTTGTGGGAAATAATCCACCACGAAGCAACATATTTTATATTCACACAAAAAGAATCCACCAAGGTCGAAATGTTCTAAAACATACGGCTATTCCTCTCTTTCCATAAAAGCCAAAGAAGAGCTCGAGCAGCACAATTCCATAAATCACTAGCTTTTCCTTTGAGCATAGATCCCCCAAAAACCTCGGTGATAAAACATTCAATTCTCCTAGGAAGACAGAAGGAAAGATCCAGTTGAGAAACAATAAAAGACCAAGCTTTTGTAGCATAGGAATAGGAATAGTGATGGAAGAGATGGTCCACCACTTCTTCATCCCTTAAACACATGCCACAGATCGAAGGGGAGAAAACCCATGTAGGACATCTCGGGTTGTGTGGTCCCTTGAAGGAAAAGGTCGGTTTTCTGAAGATGGTGGCATGTAGATCTTCTTTACGATCTCCCCTCATCTCAGCTATATGGTTCAAGGTCCCGAAGAAGATCAAAATTTTTCTTTGGTCTCTTGCTCATAGAAGCCTTAACACCCAAGAGAAATTACAGAGAAAGTGCAAAAATTGGGTGTTATCCCCATCGGTGTGTTCCCTCTGTCTGATGCATAGTGAAGATTTAGACATTTGTTTCTTCACTGTCCTTTTGCGACCAAGAGTTGGCTTTTCCACTTAGAAACTTTTGATATCTCTATCTGCTTTTCAAAAAAGATTGATGATTGGATGAATGAGGTTTTATTTGGTTGGGATCTTCCTAAGAAGACTACTTATGTTTGGAGATGTGCTTCAAGAGCTCTTCTTTGGAATTTTTAGCTTGAAAGGAATTCTCGCGTTTTTGAAGATAAGCCTTCTACTTTTGAGATTTTTTGTAGGACTGTACAGCACACGACCTCTTGGTGGTGTATCAATTACAGTAAATTCTTTTGTAACTACGACCTTTCCATGTTTATGCACAATTGGAATACGGTTGGTTAGATTGTTGTTCAGTTTTTGGTGGGGTTTTCTCTACCCTAGGCTTTAGTCTGTACCTTCTTTTTCAATACAAAAATGTTTCTTATCTAAAAAAAAAATGATCAAAGGGGGTCTTCTTCCACCAATTTTTCACCAATAACTTGAGCTAATTTTTTTCCTTTATTTTTAGTTTCTCTTATTTCCTTACTTTTAAAGTCTCGAAAACAACTTAGGATGAAAACCCAACCTAATTAAGTAATAGTATCTATTAAACACAAAATTGAAAATTTAGGAACTTACTAGAATTATTTTAAAATGTTGAGATTAAACAGAAAAAGTTTAGGAACTATTAGAGGCTTTTTAGAATATAAAGATTAAATAGACACAAGCTTGGAAGTTCGAGGGCCGAAGGTATAATTTAGCCTAAAATTTAAACTCTTTTTAAAGGAAGAGCAATTTCAACTTTAATACCATTCCCAAAATATCTTGACTTTAGTGCCAAAAGCAACTAAACTTATTAGGTAAGTTATAAGTTTGATCCTTGAACTTTCAATGGTGTGTCTCATAGATCTCAAAACTTTTACCATTGTGCCTAATAGGTTTTTGGTATTTTAATTTTATATCTAATAAGTACATAACTTTCAATTTTGTATCTAAAGATCACTAACTTATTAGACACTTTTTAAAGTTCAGGGACCTATTAAACTCAAAATTGAAAGTACAAGAATCTATTAAACTTAAACCCTTTTAAAAGTTTAGGCTTGTTCGACCAAACCGTAATTTAACTGACTTATTTTTATGTTTACAGCACTTTGTGAGAGTTGTATCAACTTGCAAGTTGAGACACTCAAGTGTCAAGTTTGCGAATGTTTGCAACCAAGTGGATTTCCAAATGTCTGTCTAATGCTGGATCAGTTTTTGAAGGAACAATTTCCTGAAGAGTATAGCCTCAGAAGAGACTCTGTTCAGCTTAAACTAGCCAATATTTTTAAGCGTGAGAGTCCAACTTCTTGTACGTGTATCTACACTTGCCTAATTTATCGTCTTTAGTGTTATGGCAGTTGTGATCCTGGAGTAATCTATTCCTTGTGCTTGTAGTGCTGTTCATTTCATTCTTATTTCCTTCTTTTTCTTTCTTAATTTCTAGTTCATATAGTTTTTGAACTTTTGTTTGTGCTTAATTTGTTTGGATTAGGAAGTTTAGCTGTCCAAACTATGTATTTGTTAGAGATGTGGGAAAATTGCTTTGTGAATCATTTTGAACATCTTTGGACCTCAAGAAGTATGTTTGTATAGCTCGTTGTATCCAATGCATGCTAGACTCCGCAACACTGACTATTGCTTGCCCATATCTTAATAAAATTTTCTTATTGTAGCTCCATGTATATCTTTAACAACGACAATACTGCATTAACATGTTCATCTCAGGTCACGTCTGTTGTGTTTATACAATTTTCATCTAAACAATTGCTTGTGCACGTGCAGCATTTAGTAGACACTTGACTGAAGACCTTGTTTAATTTGATACTCTTATTCATTTTTATTTATTTATTTCCATTTTCAGAAATCATTGGACCATTTAGAATTTCTGGTTTTTCTGTAAAATCTTTGAAATGATGCAGTTTGCTTTGTTCGGTTTACCTGTGTTTTTAGGTCCATTTTATTGATAGAACTTAGAAGAGTTATTCAACTACTATTCATACCATATTCATCTCTTTCCTATTGGTAGTTTCAGGAAACCATATTCTATACACATAAGAATTTGAAAATGTGGTGTTATTACAATTTTTTTTCTGCAATACTTTGGATTAGGTAGAAATGTACTTGTAATGCTTGATGACATATGTGTGCTCAATAATGTAGTGAATTATTCATTGTGTCATTCCACTAATACAAAAATAGTCTGTGTATTATTATTTTGGTGTTGAGTTTGTTATTTGCTTTCAGGTTCCAATGAAAGGGGCAAGAAGGGTGAAGATCTTTCAAGATCGGGTGAAGTTGCTTCTAAAGTGCATTATGGAGCTGGATGCGATTCTTGTGGGGTAATTTCATTGTATTACCAACATTCCTCTCGTACTGTCTCCTGGATCTTTGTTGCTGAACTTATGAGTCAAACCACTGTTTTAAAAATGTTGCTTTTGGAGAAGCATGGTAAGGACGCCTCGCTTCTTGAAGCAAGGTGTATTGAGGCACACACTTCTCTGTATGTTTGCACACATGTTCCAATGCACCTTGACTCATTCCTTTTTTTCTTTTCGTTTTTAATTATTTTTATACATAAATCAATCATACTTCTATAAAATGTTTAATTTTTCTCATTAATCTCTTAAAAACTGTCTAAGATTTATTTACATTTTTCAATTGTGCTTGTCACAACTAAAGTGCTTGAGCTTCGGAGCACTATTGTGCTTTAGTTTGCCTCACAATTTTAAATATATTGGTTTAAACGAAAATGTTTAATCCCATCCATCTTAAATGAGGCTGAGGACATTCTGTATGGAGACTTGCTGGTTTATTGTGCACAACTAAATTTTCTGACTGGGATTTACAACGTTTAGCAGTTGTCTGCCATTATTTGGAGATAAATAGACATTGAGTTCCATTGATCAGGGACTCCTGGAAGGATTTCAAGTTGGAAATGATAGGATGTATATCTCTTCCCTTCAGTTTTTGGACGATACCATTTTATTTTGTGCGAAGGATTGAACCAAGATATAGATAACTTGTTTAGTATGTTGAAACTATTTGAATAGATCTCGGGCCTCAAAATTAATCAGGAAATATCCCTAACGTGTTTCATAAACATGGAAACAAGTGAAGTTACAAAGTTGGCTGATGAGTTGGGATGACTCAAAGTGAACTCCCTATGTCTTGGATGAGCCGTTGGAGGATAATCCCAAGAGGGTGGTGTGGGTGCTAAGAAAGCTTGGTAACTGGAAAATTTTTCGGCTATAAATGTTGGTCAATCTTACTCTTAACTAAAACAGCCTTACCTACCAACATGAGCATAGTTCAGCGGTTAAGACATCTTGCTTCATCCCTAGAGATCGGAGGTACAATCTCCCATCCCCCACATTTGTTGTACTATAAAAAGCAAGCAGCCATAGCTAACTTACATACCTAAAGAAAGAAGATAAGGAAACAGTTGAAGCCATATTGCTAAACTGAGGCATGTTATTGGAAATTCTCCATCCATCTGTTGGTAATGGAACATCGATTATTTTTGGCAAGATAGATGGGTCGGACCCCAACCTTTGATGAGTGGAGTTGGATTCTTTTGAAATTACCCCTTTAGAAAATTGTATGGTCATCAAAGTGTGTGAGGACGCAAGTTGGGTAGAATCTGATGGCTAGAAGCCTAGAAGAAAACGACAGAATGGGAAGCTGAATGATTTTGAAGGTATTCTAGAACTCCTAAGTGGTGTACGGTTGAACTGGGGGATGGTAGGAGAACTAGGAATCTCGACTCTTCAGGCCATTTCAGTTGAATCATTCTTTCCTAAACTGGTTGGTAAAAAAGTCAGTTTGCATGAGGAGGTCGCTTCAGCTTTATGGAAAGCCAAGGTGTCTAAGCAAAGTAAAACTTCTTTTCTTGGACTGTTTGTAATGGAGTGATTAATATGTTAAGTATCCAAGATTGTATTCAAATATGGAAATAACAAACTTGGTGAAAATCACCATACAAAATTCTCAGCCGCTCGAGATGGCTTTAACTCTCCTCATTCTTGGTCCTTACCCAAAATGATAACTACCCTCCCTATTCACGTTCCCTCTCTATGTAACATAGGCAAACTTGCTACTAGTTGTACTTCTTCCTTTTTTACCCCTCCTAGTATAAACGTTACTCACATTAGCTCGGCTTTGTAAAGAAAATTAGGAGCCAGCTAGGTTGTCCCTTGTTTCTTTCTCCTCCTCCCACCCTTTGAAATGTTACATTTTTTCACCTGTTGCTCTTTAAAAAATGTTGTGGATGAGTGTAATGAAATCTTTACTTGGGGTCTTTGGCTTGGAGGTAACAATAGACGTTTTAATGAAAAGGAAAGAGAATGCAAGTTTTTGACTTGACTAGTTTCATCGTGTTGCTTAGTGTTCACTTCAAATTCTTTTTGTTGGTCTTCTTTTGTTAATGACTCAAATTGGAGTGTCTTTTTGCAAACTCGTGTTGGGATGACTTTGTTTCTGGTCTGTCTATCATTTCAATGAAAAATTAAAAAGAAAAGTTCTCTTATTCATTATGCCTAATAAAATAGTTGCTTTTTATACTCTGCCAACTTGATGAATATATTTATATATAGTGTTGAACAGGAAGTCTCATGTAACCCCATAGCTAATATGCCTGACAAAAATGGGTCTAGATATTTAAACTACTTTACAGTTCAGAATTTCATTGCCAAATTAGATCACTTGGCTAGTGAGTGATATTACTCTTATTGTTCACTGCAGATGTGTCCAATCGTTGGTGATAGATACAAGTGCAAGGATTGTTATGAGAAAATAGGTTTCGACCTCTGTGGCGATTGCTATAATACATGTTCGAAGCGTCCCGGTCGGTTCAATCAGCAACATAGACCAGAGCACAAGTTTGAGCTTATAGGACCTAGTAATGTTATGTTTCCAAATTTGATGCTGGAGGTAGTGACAGGAATGTTAGAGGAAGGTCCTAATGACGACGGTTCAGAAACCTCAGAAGACATGGGTGGCGTTCTCGATTCATCTAGCGATGCTCATGAGAACGATGAGGTCGACTCTGATGCTAGAACCCGTGCTAACTCCAACGATAATAGAACGGATCAATTTGACTCTAATCCTACTTAA

mRNA sequence

ATGGGCTACTTCTCTCCGCAATTTGATGCCCTTGCATGTGACTCACAAGCTGGTAAAAAAGTGGAACATTCAGAAGATTCTGCTAATGGCGAGCTGAAGACAAATACCAATAATGACAAAGTTGTTGCTGAACGCATATTAGAAGAAAATTCTGCAGTTGTCAGTCCTTATGGTCAAAAGACGCAAAACCATGAGGAGGTTTCAGTTGCTGACTTGCTATGTCGTGCATGCACCCAATTGCTCTTCCGACCTGTGGTTCTTAATTGTGGCCATGCACTTTGTGAGAGTTGTATCAACTTGCAAGTTGAGACACTCAAGTGTCAAGTTTGCGAATGTTTGCAACCAAGTGGATTTCCAAATGTCTGTCTAATGCTGGATCAGTTTTTGAAGGAACAATTTCCTGAAGAGTATAGCCTCAGAAGAGACTCTGTTCAGCTTAAACTAGCCAATATTTTTAAGCGTGAGAGTCCAACTTCTTGTTCCAATGAAAGGGGCAAGAAGGGTGAAGATCTTTCAAGATCGGGTGAAGTTGCTTCTAAAGTGCATTATGGAGCTGGATGCGATTCTTGTGGGGTAATTTCATTGTATTACCAACATTCCTCTCGTACTGTCTCCTGGATCTTTGTTGCTGAACTTATGAGTCAAACCACTGTTTTAAAAATGTTGCTTTTGGAGAAGCATGGTATTCTAGAACTCCTAAGTGGTGTACGGTTGAACTGGGGGATGGTAGGAGAACTAGGAATCTCGACTCTTCAGGCCATTTCAGTTGAATCATTCTTTCCTAAACTGATGTGTCCAATCGTTGGTGATAGATACAAGTGCAAGGATTGTTATGAGAAAATAGGTTTCGACCTCTGTGGCGATTGCTATAATACATGTTCGAAGCGTCCCGGTCGGTTCAATCAGCAACATAGACCAGAGCACAAGTTTGAGCTTATAGGACCTAGTAATGTTATGTTTCCAAATTTGATGCTGGAGGTAGTGACAGGAATGTTAGAGGAAGGTCCTAATGACGACGGTTCAGAAACCTCAGAAGACATGGGTGGCGTTCTCGATTCATCTAGCGATGCTCATGAGAACGATGAGGTCGACTCTGATGCTAGAACCCGTGCTAACTCCAACGATAATAGAACGGATCAATTTGACTCTAATCCTACTTAA

Coding sequence (CDS)

ATGGGCTACTTCTCTCCGCAATTTGATGCCCTTGCATGTGACTCACAAGCTGGTAAAAAAGTGGAACATTCAGAAGATTCTGCTAATGGCGAGCTGAAGACAAATACCAATAATGACAAAGTTGTTGCTGAACGCATATTAGAAGAAAATTCTGCAGTTGTCAGTCCTTATGGTCAAAAGACGCAAAACCATGAGGAGGTTTCAGTTGCTGACTTGCTATGTCGTGCATGCACCCAATTGCTCTTCCGACCTGTGGTTCTTAATTGTGGCCATGCACTTTGTGAGAGTTGTATCAACTTGCAAGTTGAGACACTCAAGTGTCAAGTTTGCGAATGTTTGCAACCAAGTGGATTTCCAAATGTCTGTCTAATGCTGGATCAGTTTTTGAAGGAACAATTTCCTGAAGAGTATAGCCTCAGAAGAGACTCTGTTCAGCTTAAACTAGCCAATATTTTTAAGCGTGAGAGTCCAACTTCTTGTTCCAATGAAAGGGGCAAGAAGGGTGAAGATCTTTCAAGATCGGGTGAAGTTGCTTCTAAAGTGCATTATGGAGCTGGATGCGATTCTTGTGGGGTAATTTCATTGTATTACCAACATTCCTCTCGTACTGTCTCCTGGATCTTTGTTGCTGAACTTATGAGTCAAACCACTGTTTTAAAAATGTTGCTTTTGGAGAAGCATGGTATTCTAGAACTCCTAAGTGGTGTACGGTTGAACTGGGGGATGGTAGGAGAACTAGGAATCTCGACTCTTCAGGCCATTTCAGTTGAATCATTCTTTCCTAAACTGATGTGTCCAATCGTTGGTGATAGATACAAGTGCAAGGATTGTTATGAGAAAATAGGTTTCGACCTCTGTGGCGATTGCTATAATACATGTTCGAAGCGTCCCGGTCGGTTCAATCAGCAACATAGACCAGAGCACAAGTTTGAGCTTATAGGACCTAGTAATGTTATGTTTCCAAATTTGATGCTGGAGGTAGTGACAGGAATGTTAGAGGAAGGTCCTAATGACGACGGTTCAGAAACCTCAGAAGACATGGGTGGCGTTCTCGATTCATCTAGCGATGCTCATGAGAACGATGAGGTCGACTCTGATGCTAGAACCCGTGCTAACTCCAACGATAATAGAACGGATCAATTTGACTCTAATCCTACTTAA

Protein sequence

MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVSPYGQKTQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPNVCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSNERGKKGEDLSRSGEVASKVHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVLKMLLLEKHGILELLSGVRLNWGMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRFNQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHENDEVDSDARTRANSNDNRTDQFDSNPT
Homology
BLAST of MS024344 vs. NCBI nr
Match: XP_022148110.1 (E3 ubiquitin-protein ligase PRT1 isoform X3 [Momordica charantia])

HSP 1 Score: 600.5 bits (1547), Expect = 1.0e-167
Identity = 310/386 (80.31%), Postives = 312/386 (80.83%), Query Frame = 0

Query: 1   MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVSPYGQK 60
           MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVV PYGQK
Sbjct: 100 MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVGPYGQK 159

Query: 61  TQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 120
           TQNHE+VSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN
Sbjct: 160 TQNHEKVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 219

Query: 121 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSNERGKKGEDLSRSGEVASK 180
           VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCS+ERGKKGEDLSRSGEVASK
Sbjct: 220 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSDERGKKGEDLSRSGEVASK 279

Query: 181 VHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVLKMLLLEKHGILELLSGVRLNW 240
           VHYGAGCDSCG                                                 
Sbjct: 280 VHYGAGCDSCG------------------------------------------------- 339

Query: 241 GMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 300
                                  MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF
Sbjct: 340 -----------------------MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 399

Query: 301 NQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 360
           NQQHRPEH FELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN
Sbjct: 400 NQQHRPEHNFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 413

Query: 361 DEVDSDARTRANSNDNRTDQFDSNPT 387
           DEVDSDARTRANSNDNRTDQFDSNPT
Sbjct: 460 DEVDSDARTRANSNDNRTDQFDSNPT 413

BLAST of MS024344 vs. NCBI nr
Match: XP_022148118.1 (E3 ubiquitin-protein ligase PRT1 isoform X4 [Momordica charantia])

HSP 1 Score: 600.5 bits (1547), Expect = 1.0e-167
Identity = 310/386 (80.31%), Postives = 312/386 (80.83%), Query Frame = 0

Query: 1   MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVSPYGQK 60
           MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVV PYGQK
Sbjct: 52  MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVGPYGQK 111

Query: 61  TQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 120
           TQNHE+VSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN
Sbjct: 112 TQNHEKVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 171

Query: 121 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSNERGKKGEDLSRSGEVASK 180
           VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCS+ERGKKGEDLSRSGEVASK
Sbjct: 172 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSDERGKKGEDLSRSGEVASK 231

Query: 181 VHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVLKMLLLEKHGILELLSGVRLNW 240
           VHYGAGCDSCG                                                 
Sbjct: 232 VHYGAGCDSCG------------------------------------------------- 291

Query: 241 GMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 300
                                  MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF
Sbjct: 292 -----------------------MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 351

Query: 301 NQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 360
           NQQHRPEH FELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN
Sbjct: 352 NQQHRPEHNFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 365

Query: 361 DEVDSDARTRANSNDNRTDQFDSNPT 387
           DEVDSDARTRANSNDNRTDQFDSNPT
Sbjct: 412 DEVDSDARTRANSNDNRTDQFDSNPT 365

BLAST of MS024344 vs. NCBI nr
Match: XP_022148093.1 (E3 ubiquitin-protein ligase PRT1 isoform X1 [Momordica charantia])

HSP 1 Score: 600.5 bits (1547), Expect = 1.0e-167
Identity = 310/386 (80.31%), Postives = 312/386 (80.83%), Query Frame = 0

Query: 1   MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVSPYGQK 60
           MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVV PYGQK
Sbjct: 133 MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVGPYGQK 192

Query: 61  TQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 120
           TQNHE+VSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN
Sbjct: 193 TQNHEKVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 252

Query: 121 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSNERGKKGEDLSRSGEVASK 180
           VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCS+ERGKKGEDLSRSGEVASK
Sbjct: 253 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSDERGKKGEDLSRSGEVASK 312

Query: 181 VHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVLKMLLLEKHGILELLSGVRLNW 240
           VHYGAGCDSCG                                                 
Sbjct: 313 VHYGAGCDSCG------------------------------------------------- 372

Query: 241 GMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 300
                                  MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF
Sbjct: 373 -----------------------MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 432

Query: 301 NQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 360
           NQQHRPEH FELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN
Sbjct: 433 NQQHRPEHNFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 446

Query: 361 DEVDSDARTRANSNDNRTDQFDSNPT 387
           DEVDSDARTRANSNDNRTDQFDSNPT
Sbjct: 493 DEVDSDARTRANSNDNRTDQFDSNPT 446

BLAST of MS024344 vs. NCBI nr
Match: XP_022148101.1 (E3 ubiquitin-protein ligase PRT1 isoform X2 [Momordica charantia])

HSP 1 Score: 580.9 bits (1496), Expect = 8.3e-162
Identity = 304/386 (78.76%), Postives = 306/386 (79.27%), Query Frame = 0

Query: 1   MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVSPYGQK 60
           MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVV PYGQK
Sbjct: 133 MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVGPYGQK 192

Query: 61  TQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 120
           TQNHE+VSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN
Sbjct: 193 TQNHEKVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 252

Query: 121 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSNERGKKGEDLSRSGEVASK 180
           VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKR      S+ERGKKGEDLSRSGEVASK
Sbjct: 253 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKR------SDERGKKGEDLSRSGEVASK 312

Query: 181 VHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVLKMLLLEKHGILELLSGVRLNW 240
           VHYGAGCDSCG                                                 
Sbjct: 313 VHYGAGCDSCG------------------------------------------------- 372

Query: 241 GMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 300
                                  MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF
Sbjct: 373 -----------------------MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 432

Query: 301 NQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 360
           NQQHRPEH FELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN
Sbjct: 433 NQQHRPEHNFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 440

Query: 361 DEVDSDARTRANSNDNRTDQFDSNPT 387
           DEVDSDARTRANSNDNRTDQFDSNPT
Sbjct: 493 DEVDSDARTRANSNDNRTDQFDSNPT 440

BLAST of MS024344 vs. NCBI nr
Match: XP_038877742.1 (E3 ubiquitin-protein ligase PRT1 isoform X2 [Benincasa hispida])

HSP 1 Score: 416.8 bits (1070), Expect = 2.1e-112
Identity = 233/400 (58.25%), Postives = 260/400 (65.00%), Query Frame = 0

Query: 1   MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVS----- 60
           +G+FSPQFD+LAC SQAG KVEH EDSANGEL T T ND VV E ILEE S VVS     
Sbjct: 52  IGFFSPQFDSLACGSQAGIKVEHLEDSANGELNTITKNDDVVTELILEETSEVVSSSSVK 111

Query: 61  -------PYGQKTQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQ 120
                  P  QKTQN E++SVAD+LC+ACTQLLFRPVVLNCGHA CESCIN QVETL+CQ
Sbjct: 112 SLNSLQDPCAQKTQNQEKISVADVLCQACTQLLFRPVVLNCGHAFCESCINSQVETLECQ 171

Query: 121 VCECLQPSGFPNVCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSNERGKKG 180
           VC+ +QP GFPNVCL LDQFLKEQFPEEYSLRRDS+QL LA   K +SP SCSNE GKKG
Sbjct: 172 VCQFVQPRGFPNVCLELDQFLKEQFPEEYSLRRDSIQLVLAKSIKHDSPISCSNEEGKKG 231

Query: 181 EDLSRSGEVASKVHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVLKMLLLEKHG 240
           +DL R GEVASKVHYG GCDSCG                                     
Sbjct: 232 KDLPRWGEVASKVHYGVGCDSCG------------------------------------- 291

Query: 241 ILELLSGVRLNWGMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYEKIGFDLCGD 300
                                              M PI+GDRYKCKDC E+IGFDLCGD
Sbjct: 292 -----------------------------------MYPIIGDRYKCKDCLEEIGFDLCGD 351

Query: 301 CYNTCSKRPGRFNQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGP------NDDGSE 360
           CYNT SKRPGRFNQQHRPEHKF+L+ P   MF N+ L VVT MLEEG       ND+GS+
Sbjct: 352 CYNTRSKRPGRFNQQHRPEHKFQLVRPQ--MFHNMRLGVVTEMLEEGTTTFIIGNDEGSD 377

Query: 361 TSEDMGGVLDSSSDAHENDEVDS--DARTRANSNDNRTDQ 381
           +S+D+G +L+ SSDA E+DE DS  D RT+ NSNDNR DQ
Sbjct: 412 SSDDIGAILELSSDARESDEDDSDVDGRTQTNSNDNRPDQ 377

BLAST of MS024344 vs. ExPASy Swiss-Prot
Match: Q8LBL5 (E3 ubiquitin-protein ligase PRT1 OS=Arabidopsis thaliana OX=3702 GN=PRT1 PE=2 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 4.9e-40
Identity = 123/382 (32.20%), Postives = 170/382 (44.50%), Query Frame = 0

Query: 4   FSPQFDAL----ACDSQA-----GKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVV 63
           FSPQ D +     C   +      +KVE   ++AN    +++  D     +  E   A  
Sbjct: 108 FSPQIDLVLDLSVCSGDSLNVSDKQKVEECSNAANLLSSSSSRGDIPCIPKNQEPTDAKA 167

Query: 64  -----SPYGQKTQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCI---NLQVETLK 123
                +   +  +  +++S  DLLC AC +LL RPVVLNCGH  CE C+     + E +K
Sbjct: 168 LNVHENELLKDNKVSKQISKDDLLCSACKELLVRPVVLNCGHVYCEGCVVDMAEESEKIK 227

Query: 124 CQVCECLQPSGFPNVCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFK-------RESPTS 183
           CQ C    P GFP VCL+L+Q L+E FPEEY+ R   VQ  LA+  K       +E P S
Sbjct: 228 CQECNVCDPRGFPKVCLILEQLLEENFPEEYNSRSSKVQKTLAHNSKGNIQSYLKEGP-S 287

Query: 184 CSNERGKKGEDLSRSGEVASKVHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVL 243
            SN+       L+  G   S VH+GAGCDSCGV                           
Sbjct: 288 LSNDNNNDDPWLANPG---SNVHFGAGCDSCGVY-------------------------- 347

Query: 244 KMLLLEKHGILELLSGVRLNWGMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYE 303
                                                         PI+GDRY+CKDC E
Sbjct: 348 ----------------------------------------------PIIGDRYRCKDCKE 407

Query: 304 KIGFDLCGDCYNTCSKRPGRFNQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGPNDD 362
           +IG+DLC DCY T SK PGRFNQQH P+H+ EL     V+     + ++ G +    +++
Sbjct: 408 EIGYDLCKDCYETPSKVPGRFNQQHTPDHRLELARSPQVLINFNSIGILLGPV---ISNE 410

BLAST of MS024344 vs. ExPASy Swiss-Prot
Match: Q9NZS9 (Bifunctional apoptosis regulator OS=Homo sapiens OX=9606 GN=BFAR PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 2.5e-07
Identity = 28/82 (34.15%), Postives = 45/82 (54.88%), Query Frame = 0

Query: 66  EVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVC-ECLQP-SGFPNVCL 125
           ++SV++  C  C  +L  P  LNCGH+ C  C+ L   + K   C EC +   GFP V +
Sbjct: 26  QISVSEFSCHCCYDILVNPTTLNCGHSFCRHCLALWWASSKKTECPECREKWEGFPKVSI 85

Query: 126 MLDQFLKEQFPEEYSLRRDSVQ 146
           +L   +++ FP+   LR + +Q
Sbjct: 86  LLRDAIEKLFPDAIRLRFEDIQ 107

BLAST of MS024344 vs. ExPASy TrEMBL
Match: A0A6J1D209 (E3 ubiquitin-protein ligase PRT1 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016854 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.9e-168
Identity = 310/386 (80.31%), Postives = 312/386 (80.83%), Query Frame = 0

Query: 1   MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVSPYGQK 60
           MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVV PYGQK
Sbjct: 100 MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVGPYGQK 159

Query: 61  TQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 120
           TQNHE+VSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN
Sbjct: 160 TQNHEKVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 219

Query: 121 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSNERGKKGEDLSRSGEVASK 180
           VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCS+ERGKKGEDLSRSGEVASK
Sbjct: 220 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSDERGKKGEDLSRSGEVASK 279

Query: 181 VHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVLKMLLLEKHGILELLSGVRLNW 240
           VHYGAGCDSCG                                                 
Sbjct: 280 VHYGAGCDSCG------------------------------------------------- 339

Query: 241 GMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 300
                                  MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF
Sbjct: 340 -----------------------MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 399

Query: 301 NQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 360
           NQQHRPEH FELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN
Sbjct: 400 NQQHRPEHNFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 413

Query: 361 DEVDSDARTRANSNDNRTDQFDSNPT 387
           DEVDSDARTRANSNDNRTDQFDSNPT
Sbjct: 460 DEVDSDARTRANSNDNRTDQFDSNPT 413

BLAST of MS024344 vs. ExPASy TrEMBL
Match: A0A6J1D375 (E3 ubiquitin-protein ligase PRT1 isoform X4 OS=Momordica charantia OX=3673 GN=LOC111016854 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.9e-168
Identity = 310/386 (80.31%), Postives = 312/386 (80.83%), Query Frame = 0

Query: 1   MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVSPYGQK 60
           MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVV PYGQK
Sbjct: 52  MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVGPYGQK 111

Query: 61  TQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 120
           TQNHE+VSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN
Sbjct: 112 TQNHEKVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 171

Query: 121 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSNERGKKGEDLSRSGEVASK 180
           VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCS+ERGKKGEDLSRSGEVASK
Sbjct: 172 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSDERGKKGEDLSRSGEVASK 231

Query: 181 VHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVLKMLLLEKHGILELLSGVRLNW 240
           VHYGAGCDSCG                                                 
Sbjct: 232 VHYGAGCDSCG------------------------------------------------- 291

Query: 241 GMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 300
                                  MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF
Sbjct: 292 -----------------------MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 351

Query: 301 NQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 360
           NQQHRPEH FELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN
Sbjct: 352 NQQHRPEHNFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 365

Query: 361 DEVDSDARTRANSNDNRTDQFDSNPT 387
           DEVDSDARTRANSNDNRTDQFDSNPT
Sbjct: 412 DEVDSDARTRANSNDNRTDQFDSNPT 365

BLAST of MS024344 vs. ExPASy TrEMBL
Match: A0A6J1D350 (E3 ubiquitin-protein ligase PRT1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016854 PE=4 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.9e-168
Identity = 310/386 (80.31%), Postives = 312/386 (80.83%), Query Frame = 0

Query: 1   MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVSPYGQK 60
           MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVV PYGQK
Sbjct: 133 MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVGPYGQK 192

Query: 61  TQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 120
           TQNHE+VSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN
Sbjct: 193 TQNHEKVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 252

Query: 121 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSNERGKKGEDLSRSGEVASK 180
           VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCS+ERGKKGEDLSRSGEVASK
Sbjct: 253 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSDERGKKGEDLSRSGEVASK 312

Query: 181 VHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVLKMLLLEKHGILELLSGVRLNW 240
           VHYGAGCDSCG                                                 
Sbjct: 313 VHYGAGCDSCG------------------------------------------------- 372

Query: 241 GMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 300
                                  MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF
Sbjct: 373 -----------------------MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 432

Query: 301 NQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 360
           NQQHRPEH FELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN
Sbjct: 433 NQQHRPEHNFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 446

Query: 361 DEVDSDARTRANSNDNRTDQFDSNPT 387
           DEVDSDARTRANSNDNRTDQFDSNPT
Sbjct: 493 DEVDSDARTRANSNDNRTDQFDSNPT 446

BLAST of MS024344 vs. ExPASy TrEMBL
Match: A0A6J1D4D0 (E3 ubiquitin-protein ligase PRT1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016854 PE=4 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 4.0e-162
Identity = 304/386 (78.76%), Postives = 306/386 (79.27%), Query Frame = 0

Query: 1   MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVSPYGQK 60
           MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVV PYGQK
Sbjct: 133 MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVGPYGQK 192

Query: 61  TQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 120
           TQNHE+VSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN
Sbjct: 193 TQNHEKVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPN 252

Query: 121 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSNERGKKGEDLSRSGEVASK 180
           VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKR      S+ERGKKGEDLSRSGEVASK
Sbjct: 253 VCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKR------SDERGKKGEDLSRSGEVASK 312

Query: 181 VHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVLKMLLLEKHGILELLSGVRLNW 240
           VHYGAGCDSCG                                                 
Sbjct: 313 VHYGAGCDSCG------------------------------------------------- 372

Query: 241 GMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 300
                                  MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF
Sbjct: 373 -----------------------MCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRF 432

Query: 301 NQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 360
           NQQHRPEH FELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN
Sbjct: 433 NQQHRPEHNFELIGPSNVMFPNLMLEVVTGMLEEGPNDDGSETSEDMGGVLDSSSDAHEN 440

Query: 361 DEVDSDARTRANSNDNRTDQFDSNPT 387
           DEVDSDARTRANSNDNRTDQFDSNPT
Sbjct: 493 DEVDSDARTRANSNDNRTDQFDSNPT 440

BLAST of MS024344 vs. ExPASy TrEMBL
Match: A0A1S3C815 (E3 ubiquitin-protein ligase PRT1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497542 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 6.3e-107
Identity = 223/406 (54.93%), Postives = 259/406 (63.79%), Query Frame = 0

Query: 1   MGYFSPQFDALACDSQAGKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVVS----- 60
           +GYFSPQFD+LAC SQAG KVEH EDSANGEL TNT N   +AE ILEENS VVS     
Sbjct: 175 IGYFSPQFDSLACGSQAGMKVEHLEDSANGELNTNTKNGDAIAELILEENSDVVSSTSVV 234

Query: 61  -------PYGQKTQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQ 120
                  P  QKTQ  E++SVAD+LC+ACTQLLFRPVV+NCGH  CESCIN QVETL+CQ
Sbjct: 235 SLNSLQDPCAQKTQKQEKISVADVLCQACTQLLFRPVVINCGHVFCESCINSQVETLECQ 294

Query: 121 VCECLQPSGFPNVCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFKRESPTSCSNERGKKG 180
           VC+ LQP GFPNVCL LDQFLKEQFPEEYS+RRDSVQL+ AN  K ++PTSCS E GKKG
Sbjct: 295 VCQSLQPRGFPNVCLELDQFLKEQFPEEYSIRRDSVQLEQANSIKHDNPTSCSIEEGKKG 354

Query: 181 EDLSRSGEVASKVHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVLKMLLLEKHG 240
           + L R G+VASKVH   GCDSCG                                     
Sbjct: 355 QYLPRWGDVASKVHSAVGCDSCG------------------------------------- 414

Query: 241 ILELLSGVRLNWGMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYEKIGFDLCGD 300
                                              M P++GDRYKCKDC E++GFDLCGD
Sbjct: 415 -----------------------------------MFPLIGDRYKCKDCLEEVGFDLCGD 474

Query: 301 CYNTCSKRPGRFNQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEG------PNDDGSE 360
           CYNT SKRPGRFNQQHRPEH+F L+ P   MF N+ L VVT MLEEG       N +GS+
Sbjct: 475 CYNTRSKRPGRFNQQHRPEHRFHLVHPP--MFRNMRLGVVTEMLEEGSTTFIIANGEGSD 506

Query: 361 TSEDMGGVLDSSSDAHENDEVDS--DARTRANSNDNRTDQFDSNPT 387
           +S+++G +L+ SSDA E+DE DS  D RT+ NS+D+RTDQ  S P+
Sbjct: 535 SSDNIGAILEFSSDARESDEDDSDVDGRTQINSSDDRTDQNYSEPS 506

BLAST of MS024344 vs. TAIR 10
Match: AT3G24800.1 (proteolysis 1 )

HSP 1 Score: 166.8 bits (421), Expect = 3.5e-41
Identity = 123/382 (32.20%), Postives = 170/382 (44.50%), Query Frame = 0

Query: 4   FSPQFDAL----ACDSQA-----GKKVEHSEDSANGELKTNTNNDKVVAERILEENSAVV 63
           FSPQ D +     C   +      +KVE   ++AN    +++  D     +  E   A  
Sbjct: 108 FSPQIDLVLDLSVCSGDSLNVSDKQKVEECSNAANLLSSSSSRGDIPCIPKNQEPTDAKA 167

Query: 64  -----SPYGQKTQNHEEVSVADLLCRACTQLLFRPVVLNCGHALCESCI---NLQVETLK 123
                +   +  +  +++S  DLLC AC +LL RPVVLNCGH  CE C+     + E +K
Sbjct: 168 LNVHENELLKDNKVSKQISKDDLLCSACKELLVRPVVLNCGHVYCEGCVVDMAEESEKIK 227

Query: 124 CQVCECLQPSGFPNVCLMLDQFLKEQFPEEYSLRRDSVQLKLANIFK-------RESPTS 183
           CQ C    P GFP VCL+L+Q L+E FPEEY+ R   VQ  LA+  K       +E P S
Sbjct: 228 CQECNVCDPRGFPKVCLILEQLLEENFPEEYNSRSSKVQKTLAHNSKGNIQSYLKEGP-S 287

Query: 184 CSNERGKKGEDLSRSGEVASKVHYGAGCDSCGVISLYYQHSSRTVSWIFVAELMSQTTVL 243
            SN+       L+  G   S VH+GAGCDSCGV                           
Sbjct: 288 LSNDNNNDDPWLANPG---SNVHFGAGCDSCGVY-------------------------- 347

Query: 244 KMLLLEKHGILELLSGVRLNWGMVGELGISTLQAISVESFFPKLMCPIVGDRYKCKDCYE 303
                                                         PI+GDRY+CKDC E
Sbjct: 348 ----------------------------------------------PIIGDRYRCKDCKE 407

Query: 304 KIGFDLCGDCYNTCSKRPGRFNQQHRPEHKFELIGPSNVMFPNLMLEVVTGMLEEGPNDD 362
           +IG+DLC DCY T SK PGRFNQQH P+H+ EL     V+     + ++ G +    +++
Sbjct: 408 EIGYDLCKDCYETPSKVPGRFNQQHTPDHRLELARSPQVLINFNSIGILLGPV---ISNE 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148110.11.0e-16780.31E3 ubiquitin-protein ligase PRT1 isoform X3 [Momordica charantia][more]
XP_022148118.11.0e-16780.31E3 ubiquitin-protein ligase PRT1 isoform X4 [Momordica charantia][more]
XP_022148093.11.0e-16780.31E3 ubiquitin-protein ligase PRT1 isoform X1 [Momordica charantia][more]
XP_022148101.18.3e-16278.76E3 ubiquitin-protein ligase PRT1 isoform X2 [Momordica charantia][more]
XP_038877742.12.1e-11258.25E3 ubiquitin-protein ligase PRT1 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8LBL54.9e-4032.20E3 ubiquitin-protein ligase PRT1 OS=Arabidopsis thaliana OX=3702 GN=PRT1 PE=2 SV... [more]
Q9NZS92.5e-0734.15Bifunctional apoptosis regulator OS=Homo sapiens OX=9606 GN=BFAR PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D2094.9e-16880.31E3 ubiquitin-protein ligase PRT1 isoform X3 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1D3754.9e-16880.31E3 ubiquitin-protein ligase PRT1 isoform X4 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1D3504.9e-16880.31E3 ubiquitin-protein ligase PRT1 isoform X1 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1D4D04.0e-16278.76E3 ubiquitin-protein ligase PRT1 isoform X2 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A1S3C8156.3e-10754.93E3 ubiquitin-protein ligase PRT1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497... [more]
Match NameE-valueIdentityDescription
AT3G24800.13.5e-4132.20proteolysis 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 74..110
e-value: 3.7E-4
score: 29.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 74..111
score: 9.833738
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 19..140
e-value: 5.8E-10
score: 40.8
IPR043145Zinc finger, ZZ-type superfamilyGENE3D3.30.60.90coord: 260..313
e-value: 4.1E-11
score: 44.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 355..375
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 332..386
NoneNo IPR availablePANTHERPTHR15898UNCHARACTERIZEDcoord: 264..375
NoneNo IPR availablePANTHERPTHR15898UNCHARACTERIZEDcoord: 16..193
NoneNo IPR availablePANTHERPTHR15898:SF15E3 UBIQUITIN-PROTEIN LIGASE PRT1-LIKEcoord: 16..193
coord: 264..375
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 264..313
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 68..113
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 89..98

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS024344.1MS024344.1mRNA