MC08g2149 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g2149 vs. ExPASy Swiss-Prot
Match: F4KDF5 (Protein MULTIPOLAR SPINDLE 1 OS=Arabidopsis thaliana OX=3702 GN=MPS1 PE=2 SV=2)

HSP 1 Score: 357.5 bits (916), Expect = 2.0e-97
Identity = 193/389 (49.61%), Postives = 271/389 (69.67%), Query Frame = 0

Query: 16  DQSLKLAIAISLLRSKVIQKQQHTVSN---PPSDSDALRWKRKAKERKQELIRLREDLKQ 75
           ++SL+LAIA+SLLRSK    Q  + ++     S+SDALRWK+KAKERK+E+IRL+EDLK 
Sbjct: 14  EESLRLAIAVSLLRSKFQNHQSSSSTSRCYVSSESDALRWKQKAKERKKEIIRLQEDLKD 73

Query: 76  AEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQNATCKCFFFDNLGKFNPKQLE 135
           AE        SSF                   H DLF  NA+CKC+FFDNLG F+ +++ 
Sbjct: 74  AE--------SSF-------------------HRDLFPANASCKCYFFDNLGVFSGRRIG 133

Query: 136 DCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEHLRASVDFLVELC 195
           + +++RFNDVLRRRFLR  R R RR+ + SS  R +  + +  +E EHLR S+DFL+EL 
Sbjct: 134 EASESRFNDVLRRRFLRLARRRSRRKLTRSS-QRLQPSEPDYEEEAEHLRISIDFLLELS 193

Query: 196 DTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIMRLIRRMCSPLK- 255
           +  S   ++NF NW+HQAVDFI ASLK L+   R+LE +E  IS +I +LI RMC+P+K 
Sbjct: 194 EADS--NDSNFSNWSHQAVDFIFASLKKLISMGRNLESVEESISFMITQLITRMCTPVKG 253

Query: 256 DDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFMDPFDDNFPSLH 315
           ++    ++ V FY Q L R+LG EP+IGQRAI ++SQRIS + E+ LFMDPFD++FP + 
Sbjct: 254 NEVKQLETSVGFYVQHLIRKLGSEPFIGQRAIFAISQRISILAENLLFMDPFDESFPEMD 313

Query: 316 ECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELLESRNGVYVLYV 375
           EC+FI+IQL+EFL+ DYL  W+ +  FDN++ EE + S++HA+KA++ LE RNG+Y+LY+
Sbjct: 314 ECMFILIQLIEFLICDYLLPWANEA-FDNVMFEEWIASVVHARKAVKALEERNGLYLLYM 371

Query: 376 DRVAGELANQISNIPRLRELKPDVLDDLI 401
           DRV GELA ++  I   RE++P +LD ++
Sbjct: 374 DRVTGELAKRVGQITSFREVEPAILDKIL 371

BLAST of MC08g2149 vs. NCBI nr
Match: XP_022158177.1 (protein MULTIPOLAR SPINDLE 1 [Momordica charantia])

HSP 1 Score: 721 bits (1860), Expect = 3.95e-261
Identity = 374/401 (93.27%), Postives = 374/401 (93.27%), Query Frame = 0

Query: 1   MSVADGGSQIPAPIDDQSLKLAIAISLLRSKVIQKQQHTVSNPPSDSDALRWKRKAKERK 60
           MSVADGGSQIPAPIDDQSLKLAIAISLLRSKVIQKQQHTVSNPPSDSDALRWKRKAKERK
Sbjct: 1   MSVADGGSQIPAPIDDQSLKLAIAISLLRSKVIQKQQHTVSNPPSDSDALRWKRKAKERK 60

Query: 61  QELIRLREDLKQAEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQNATCKCFFF 120
           QELIRLREDLKQAE                           DPSHGDLFSQNATCKCFFF
Sbjct: 61  QELIRLREDLKQAE---------------------------DPSHGDLFSQNATCKCFFF 120

Query: 121 DNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEH 180
           DNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEH
Sbjct: 121 DNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEH 180

Query: 181 LRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIM 240
           LRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIM
Sbjct: 181 LRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIM 240

Query: 241 RLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFM 300
           RLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFM
Sbjct: 241 RLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFM 300

Query: 301 DPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELL 360
           DPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELL
Sbjct: 301 DPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELL 360

Query: 361 ESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 401
           ESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH
Sbjct: 361 ESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 374

BLAST of MC08g2149 vs. NCBI nr
Match: XP_008452708.1 (PREDICTED: uncharacterized protein LOC103493647 isoform X1 [Cucumis melo] >XP_016900949.1 PREDICTED: uncharacterized protein LOC103493647 isoform X1 [Cucumis melo] >TYJ96404.1 Multipolar spindle 1, putative isoform 1 [Cucumis melo var. makuwa])

HSP 1 Score: 583 bits (1503), Expect = 2.78e-206
Identity = 310/401 (77.31%), Postives = 344/401 (85.79%), Query Frame = 0

Query: 8   SQIPAP---IDDQSLKLAIAISLLRSKVIQKQ--QH--TVSNPPSDSDALRWKRKAKERK 67
           SQIPA    IDDQSLKLAIA SLL SK IQ Q  QH  TVS+PPS S+ALRWKRKAKERK
Sbjct: 4   SQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSSPPSHSNALRWKRKAKERK 63

Query: 68  QELIRLREDLKQAEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQNATCKCFFF 127
           QELIRLRE+LKQAEGLC  IS SS  LS ++I    D   LD SH D  SQN+TCKCFFF
Sbjct: 64  QELIRLRENLKQAEGLCTIISSSSLLLSSELIGFLSDDLALDASHDDFLSQNSTCKCFFF 123

Query: 128 DNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEH 187
           DNLGK NP + ED TDNRFNDVLRRRFLRQVRYRERRRKSD++++RKRLLDFNE DEVE 
Sbjct: 124 DNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLLDFNEDDEVER 183

Query: 188 LRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIM 247
           L+ASVDFLVEL DT+SPVGE NFKNWAHQA DFILASLKDLLP+ R +E IEGIISSLIM
Sbjct: 184 LQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSLIM 243

Query: 248 RLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFM 307
           RLIR+M SP+KD+  DS SDV+FY Q L RELGRE YIGQRAI+SVSQRISAVGES LFM
Sbjct: 244 RLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLLFM 303

Query: 308 DPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELL 367
           DPFDDNFPSLHE LFIMIQL+EFL+SDYL MWS D+NFD+LL EE +ISI+HAKKALELL
Sbjct: 304 DPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDRNFDSLLFEEGMISIVHAKKALELL 363

Query: 368 ESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 401
           ESRNG+Y+LY+DRV G+LANQISNIPRLRE+KPD++DDL+H
Sbjct: 364 ESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 404

BLAST of MC08g2149 vs. NCBI nr
Match: XP_038890473.1 (protein MULTIPOLAR SPINDLE 1 isoform X1 [Benincasa hispida] >XP_038890474.1 protein MULTIPOLAR SPINDLE 1 isoform X1 [Benincasa hispida] >XP_038890475.1 protein MULTIPOLAR SPINDLE 1 isoform X1 [Benincasa hispida] >XP_038890476.1 protein MULTIPOLAR SPINDLE 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 582 bits (1500), Expect = 3.84e-206
Identity = 310/409 (75.79%), Postives = 342/409 (83.62%), Query Frame = 0

Query: 1   MSVADGGSQIPAP---IDDQSLKLAIAISLLRSKVIQKQQH----TVSNPPS-DSDALRW 60
           MSVA+G SQIPA    IDDQSLKLA+AISLLRSK IQ QQ     TVS+PPS DSDALRW
Sbjct: 1   MSVANGRSQIPAASGGIDDQSLKLAVAISLLRSKAIQNQQRQNPPTVSSPPSSDSDALRW 60

Query: 61  KRKAKERKQELIRLREDLKQAEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQN 120
           KRKAKERKQELIRLRE LKQAE                          +D SH DL SQN
Sbjct: 61  KRKAKERKQELIRLREVLKQAEA-------------------------VDASHDDLLSQN 120

Query: 121 ATCKCFFFDNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDF 180
           ATCKCFFFDN+GK NPK+LED TDNRFNDVLRRRFLRQVRYRER RK+D++++RKRLLDF
Sbjct: 121 ATCKCFFFDNMGKLNPKRLEDGTDNRFNDVLRRRFLRQVRYRERGRKADNAVHRKRLLDF 180

Query: 181 NEVDEVEHLRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIE 240
           NE DEVE L+ASVDFLVELCDT+SPVGEANFKNWAHQAVDFILASLKDLLP+ R++E IE
Sbjct: 181 NEDDEVERLQASVDFLVELCDTYSPVGEANFKNWAHQAVDFILASLKDLLPKGRNMELIE 240

Query: 241 GIISSLIMRLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISA 300
           GIISSLIMRL+R+M SP+KD+ GDS SD+RFYTQQL RELGRE YIGQRAI+ VSQRISA
Sbjct: 241 GIISSLIMRLVRKMGSPVKDELGDSVSDIRFYTQQLIRELGRETYIGQRAIVYVSQRISA 300

Query: 301 VGESFLFMDPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILH 360
            GES LFMDPFDDN+PSLHECLFIMIQL+EF+ SDYL MWS D+NFD LL EE +ISILH
Sbjct: 301 AGESLLFMDPFDDNYPSLHECLFIMIQLIEFMTSDYLQMWSKDENFDRLLFEEWMISILH 360

Query: 361 AKKALELLESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 401
           AKKALELLESRNG+YVLY+DRV  ELANQISNI RLRELKPD++DDLI+
Sbjct: 361 AKKALELLESRNGLYVLYMDRVTNELANQISNIRRLRELKPDIVDDLIN 384

BLAST of MC08g2149 vs. NCBI nr
Match: XP_022969582.1 (protein MULTIPOLAR SPINDLE 1 [Cucurbita maxima])

HSP 1 Score: 581 bits (1497), Expect = 9.84e-206
Identity = 308/408 (75.49%), Postives = 339/408 (83.09%), Query Frame = 0

Query: 1   MSVADGGSQIPAP---IDDQSLKLAIAISLLRSKVIQKQQH----TVSNPPSDSDALRWK 60
           MSVAD  SQ+P     IDD+SLKLAIAISLLRSK IQKQQ      VS+PPSDSDALRWK
Sbjct: 1   MSVADARSQMPVANGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSHPPSDSDALRWK 60

Query: 61  RKAKERKQELIRLREDLKQAEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQNA 120
           +KAKERKQELIRLREDLKQAE                           D SH DLFSQNA
Sbjct: 61  QKAKERKQELIRLREDLKQAE---------------------------DASHDDLFSQNA 120

Query: 121 TCKCFFFDNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFN 180
           TCKCFFFDNL K NPK+LE   DNRFNDVLRRRFLRQVRYRERRRK DS ++RKRLLDFN
Sbjct: 121 TCKCFFFDNLAKSNPKRLEHDIDNRFNDVLRRRFLRQVRYRERRRKLDSGVHRKRLLDFN 180

Query: 181 EVDEVEHLRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEG 240
           EVDEVEHLR+SVDFLVELCDTFSPVGEA FK WAHQAV+FILASLKDLLPR R++E IEG
Sbjct: 181 EVDEVEHLRSSVDFLVELCDTFSPVGEATFKIWAHQAVNFILASLKDLLPRGRNVELIEG 240

Query: 241 IISSLIMRLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAV 300
           I++SLIMRLIRRMCSPLKD+S +S  + RFYTQQL RELGREP+IGQRA+ISVSQRISA 
Sbjct: 241 IVTSLIMRLIRRMCSPLKDESENSVFNTRFYTQQLVRELGREPFIGQRAVISVSQRISAA 300

Query: 301 GESFLFMDPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHA 360
           GES LFMDPFDDNFPSLHE LF+MIQL+EFL+ DYLP+WS D+NF++LL EE VISILHA
Sbjct: 301 GESLLFMDPFDDNFPSLHESLFVMIQLIEFLILDYLPIWSKDENFNSLLFEEWVISILHA 360

Query: 361 KKALELLESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 401
           KKAL+LLESRNGVY+LY+DRV GELANQ+SNI  LRELKPD+LDDL+H
Sbjct: 361 KKALKLLESRNGVYLLYMDRVTGELANQVSNIQGLRELKPDILDDLVH 381

BLAST of MC08g2149 vs. NCBI nr
Match: XP_038890478.1 (protein MULTIPOLAR SPINDLE 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 580 bits (1496), Expect = 1.45e-205
Identity = 310/409 (75.79%), Postives = 341/409 (83.37%), Query Frame = 0

Query: 1   MSVADGGSQIPAP---IDDQSLKLAIAISLLRSKVIQKQQH----TVSNPPS-DSDALRW 60
           MSVA+G SQIPA    IDDQSLKLA+AISLLRSK IQ QQ     TVS+PPS DSDALRW
Sbjct: 1   MSVANGRSQIPAASGGIDDQSLKLAVAISLLRSKAIQNQQRQNPPTVSSPPSSDSDALRW 60

Query: 61  KRKAKERKQELIRLREDLKQAEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQN 120
           KRKAKERKQELIRLRE LKQAE                           D SH DL SQN
Sbjct: 61  KRKAKERKQELIRLREVLKQAE---------------------------DASHDDLLSQN 120

Query: 121 ATCKCFFFDNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDF 180
           ATCKCFFFDN+GK NPK+LED TDNRFNDVLRRRFLRQVRYRER RK+D++++RKRLLDF
Sbjct: 121 ATCKCFFFDNMGKLNPKRLEDGTDNRFNDVLRRRFLRQVRYRERGRKADNAVHRKRLLDF 180

Query: 181 NEVDEVEHLRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIE 240
           NE DEVE L+ASVDFLVELCDT+SPVGEANFKNWAHQAVDFILASLKDLLP+ R++E IE
Sbjct: 181 NEDDEVERLQASVDFLVELCDTYSPVGEANFKNWAHQAVDFILASLKDLLPKGRNMELIE 240

Query: 241 GIISSLIMRLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISA 300
           GIISSLIMRL+R+M SP+KD+ GDS SD+RFYTQQL RELGRE YIGQRAI+ VSQRISA
Sbjct: 241 GIISSLIMRLVRKMGSPVKDELGDSVSDIRFYTQQLIRELGRETYIGQRAIVYVSQRISA 300

Query: 301 VGESFLFMDPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILH 360
            GES LFMDPFDDN+PSLHECLFIMIQL+EF+ SDYL MWS D+NFD LL EE +ISILH
Sbjct: 301 AGESLLFMDPFDDNYPSLHECLFIMIQLIEFMTSDYLQMWSKDENFDRLLFEEWMISILH 360

Query: 361 AKKALELLESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 401
           AKKALELLESRNG+YVLY+DRV  ELANQISNI RLRELKPD++DDLI+
Sbjct: 361 AKKALELLESRNGLYVLYMDRVTNELANQISNIRRLRELKPDIVDDLIN 382

BLAST of MC08g2149 vs. ExPASy TrEMBL
Match: A0A6J1DWJ3 (protein MULTIPOLAR SPINDLE 1 OS=Momordica charantia OX=3673 GN=LOC111024716 PE=4 SV=1)

HSP 1 Score: 721 bits (1860), Expect = 1.91e-261
Identity = 374/401 (93.27%), Postives = 374/401 (93.27%), Query Frame = 0

Query: 1   MSVADGGSQIPAPIDDQSLKLAIAISLLRSKVIQKQQHTVSNPPSDSDALRWKRKAKERK 60
           MSVADGGSQIPAPIDDQSLKLAIAISLLRSKVIQKQQHTVSNPPSDSDALRWKRKAKERK
Sbjct: 1   MSVADGGSQIPAPIDDQSLKLAIAISLLRSKVIQKQQHTVSNPPSDSDALRWKRKAKERK 60

Query: 61  QELIRLREDLKQAEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQNATCKCFFF 120
           QELIRLREDLKQAE                           DPSHGDLFSQNATCKCFFF
Sbjct: 61  QELIRLREDLKQAE---------------------------DPSHGDLFSQNATCKCFFF 120

Query: 121 DNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEH 180
           DNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEH
Sbjct: 121 DNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEH 180

Query: 181 LRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIM 240
           LRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIM
Sbjct: 181 LRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIM 240

Query: 241 RLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFM 300
           RLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFM
Sbjct: 241 RLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFM 300

Query: 301 DPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELL 360
           DPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELL
Sbjct: 301 DPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELL 360

Query: 361 ESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 401
           ESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH
Sbjct: 361 ESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 374

BLAST of MC08g2149 vs. ExPASy TrEMBL
Match: A0A1S3BUI0 (uncharacterized protein LOC103493647 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493647 PE=4 SV=1)

HSP 1 Score: 583 bits (1503), Expect = 1.35e-206
Identity = 310/401 (77.31%), Postives = 344/401 (85.79%), Query Frame = 0

Query: 8   SQIPAP---IDDQSLKLAIAISLLRSKVIQKQ--QH--TVSNPPSDSDALRWKRKAKERK 67
           SQIPA    IDDQSLKLAIA SLL SK IQ Q  QH  TVS+PPS S+ALRWKRKAKERK
Sbjct: 4   SQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSSPPSHSNALRWKRKAKERK 63

Query: 68  QELIRLREDLKQAEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQNATCKCFFF 127
           QELIRLRE+LKQAEGLC  IS SS  LS ++I    D   LD SH D  SQN+TCKCFFF
Sbjct: 64  QELIRLRENLKQAEGLCTIISSSSLLLSSELIGFLSDDLALDASHDDFLSQNSTCKCFFF 123

Query: 128 DNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEH 187
           DNLGK NP + ED TDNRFNDVLRRRFLRQVRYRERRRKSD++++RKRLLDFNE DEVE 
Sbjct: 124 DNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLLDFNEDDEVER 183

Query: 188 LRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIM 247
           L+ASVDFLVEL DT+SPVGE NFKNWAHQA DFILASLKDLLP+ R +E IEGIISSLIM
Sbjct: 184 LQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSLIM 243

Query: 248 RLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFM 307
           RLIR+M SP+KD+  DS SDV+FY Q L RELGRE YIGQRAI+SVSQRISAVGES LFM
Sbjct: 244 RLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLLFM 303

Query: 308 DPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELL 367
           DPFDDNFPSLHE LFIMIQL+EFL+SDYL MWS D+NFD+LL EE +ISI+HAKKALELL
Sbjct: 304 DPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDRNFDSLLFEEGMISIVHAKKALELL 363

Query: 368 ESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 401
           ESRNG+Y+LY+DRV G+LANQISNIPRLRE+KPD++DDL+H
Sbjct: 364 ESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 404

BLAST of MC08g2149 vs. ExPASy TrEMBL
Match: A0A5D3BBS1 (Multipolar spindle 1, putative isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00310 PE=4 SV=1)

HSP 1 Score: 583 bits (1503), Expect = 1.35e-206
Identity = 310/401 (77.31%), Postives = 344/401 (85.79%), Query Frame = 0

Query: 8   SQIPAP---IDDQSLKLAIAISLLRSKVIQKQ--QH--TVSNPPSDSDALRWKRKAKERK 67
           SQIPA    IDDQSLKLAIA SLL SK IQ Q  QH  TVS+PPS S+ALRWKRKAKERK
Sbjct: 4   SQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSSPPSHSNALRWKRKAKERK 63

Query: 68  QELIRLREDLKQAEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQNATCKCFFF 127
           QELIRLRE+LKQAEGLC  IS SS  LS ++I    D   LD SH D  SQN+TCKCFFF
Sbjct: 64  QELIRLRENLKQAEGLCTIISSSSLLLSSELIGFLSDDLALDASHDDFLSQNSTCKCFFF 123

Query: 128 DNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEH 187
           DNLGK NP + ED TDNRFNDVLRRRFLRQVRYRERRRKSD++++RKRLLDFNE DEVE 
Sbjct: 124 DNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLLDFNEDDEVER 183

Query: 188 LRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIM 247
           L+ASVDFLVEL DT+SPVGE NFKNWAHQA DFILASLKDLLP+ R +E IEGIISSLIM
Sbjct: 184 LQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSLIM 243

Query: 248 RLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFM 307
           RLIR+M SP+KD+  DS SDV+FY Q L RELGRE YIGQRAI+SVSQRISAVGES LFM
Sbjct: 244 RLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLLFM 303

Query: 308 DPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELL 367
           DPFDDNFPSLHE LFIMIQL+EFL+SDYL MWS D+NFD+LL EE +ISI+HAKKALELL
Sbjct: 304 DPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDRNFDSLLFEEGMISIVHAKKALELL 363

Query: 368 ESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 401
           ESRNG+Y+LY+DRV G+LANQISNIPRLRE+KPD++DDL+H
Sbjct: 364 ESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 404

BLAST of MC08g2149 vs. ExPASy TrEMBL
Match: A0A6J1HWP8 (protein MULTIPOLAR SPINDLE 1 OS=Cucurbita maxima OX=3661 GN=LOC111468560 PE=4 SV=1)

HSP 1 Score: 581 bits (1497), Expect = 4.76e-206
Identity = 308/408 (75.49%), Postives = 339/408 (83.09%), Query Frame = 0

Query: 1   MSVADGGSQIPAP---IDDQSLKLAIAISLLRSKVIQKQQH----TVSNPPSDSDALRWK 60
           MSVAD  SQ+P     IDD+SLKLAIAISLLRSK IQKQQ      VS+PPSDSDALRWK
Sbjct: 1   MSVADARSQMPVANGGIDDRSLKLAIAISLLRSKAIQKQQQQQQPVVSHPPSDSDALRWK 60

Query: 61  RKAKERKQELIRLREDLKQAEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQNA 120
           +KAKERKQELIRLREDLKQAE                           D SH DLFSQNA
Sbjct: 61  QKAKERKQELIRLREDLKQAE---------------------------DASHDDLFSQNA 120

Query: 121 TCKCFFFDNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFN 180
           TCKCFFFDNL K NPK+LE   DNRFNDVLRRRFLRQVRYRERRRK DS ++RKRLLDFN
Sbjct: 121 TCKCFFFDNLAKSNPKRLEHDIDNRFNDVLRRRFLRQVRYRERRRKLDSGVHRKRLLDFN 180

Query: 181 EVDEVEHLRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEG 240
           EVDEVEHLR+SVDFLVELCDTFSPVGEA FK WAHQAV+FILASLKDLLPR R++E IEG
Sbjct: 181 EVDEVEHLRSSVDFLVELCDTFSPVGEATFKIWAHQAVNFILASLKDLLPRGRNVELIEG 240

Query: 241 IISSLIMRLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAV 300
           I++SLIMRLIRRMCSPLKD+S +S  + RFYTQQL RELGREP+IGQRA+ISVSQRISA 
Sbjct: 241 IVTSLIMRLIRRMCSPLKDESENSVFNTRFYTQQLVRELGREPFIGQRAVISVSQRISAA 300

Query: 301 GESFLFMDPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHA 360
           GES LFMDPFDDNFPSLHE LF+MIQL+EFL+ DYLP+WS D+NF++LL EE VISILHA
Sbjct: 301 GESLLFMDPFDDNFPSLHESLFVMIQLIEFLILDYLPIWSKDENFNSLLFEEWVISILHA 360

Query: 361 KKALELLESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 401
           KKAL+LLESRNGVY+LY+DRV GELANQ+SNI  LRELKPD+LDDL+H
Sbjct: 361 KKALKLLESRNGVYLLYMDRVTGELANQVSNIQGLRELKPDILDDLVH 381

BLAST of MC08g2149 vs. ExPASy TrEMBL
Match: A0A5A7ULP1 (Multipolar spindle 1, putative isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold323G00780 PE=4 SV=1)

HSP 1 Score: 579 bits (1493), Expect = 4.47e-205
Identity = 308/401 (76.81%), Postives = 343/401 (85.54%), Query Frame = 0

Query: 8   SQIPAP---IDDQSLKLAIAISLLRSKVIQKQ--QH--TVSNPPSDSDALRWKRKAKERK 67
           +QIPA    IDDQSLKLAIA SLL SK IQ Q  QH  TVS+PPS S+ALRWKRKAKERK
Sbjct: 4   TQIPAASGGIDDQSLKLAIAGSLLHSKAIQNQHQQHSPTVSSPPSHSNALRWKRKAKERK 63

Query: 68  QELIRLREDLKQAEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQNATCKCFFF 127
           QELIRLRE+LKQAEGLC  IS SS  LS ++I    D   LD SH D  SQN+TCKCFFF
Sbjct: 64  QELIRLRENLKQAEGLCTIISSSSLLLSSELIGFLSDDLALDASHDDFLSQNSTCKCFFF 123

Query: 128 DNLGKFNPKQLEDCTDNRFNDVLRRRFLRQVRYRERRRKSDSSINRKRLLDFNEVDEVEH 187
           DNLGK NP + ED TDNRFNDVLRRRFLRQVRYRERRRKSD++++RKRL DFNE DEVE 
Sbjct: 124 DNLGKLNPTRPEDGTDNRFNDVLRRRFLRQVRYRERRRKSDNAVHRKRLSDFNEDDEVER 183

Query: 188 LRASVDFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIM 247
           L+ASVDFLVEL DT+SPVGE NFKNWAHQA DFILASLKDLLP+ R +E IEGIISSLIM
Sbjct: 184 LQASVDFLVELYDTYSPVGEVNFKNWAHQAADFILASLKDLLPKGRYVELIEGIISSLIM 243

Query: 248 RLIRRMCSPLKDDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFM 307
           RLIR+M SP+KD+  DS SDV+FY Q L RELGRE YIGQRAI+SVSQRISAVGES LFM
Sbjct: 244 RLIRKMHSPVKDELEDSASDVQFYAQHLIRELGRETYIGQRAIVSVSQRISAVGESLLFM 303

Query: 308 DPFDDNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELL 367
           DPFDDNFPSLHE LFIMIQL+EFL+SDYL MWS D+NFD+LL EE +ISI+HAKKALELL
Sbjct: 304 DPFDDNFPSLHEYLFIMIQLIEFLISDYLKMWSKDQNFDSLLFEEGMISIVHAKKALELL 363

Query: 368 ESRNGVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLIH 401
           ESRNG+Y+LY+DRV G+LANQISNIPRLRE+KPD++DDL+H
Sbjct: 364 ESRNGLYILYMDRVTGDLANQISNIPRLREIKPDIVDDLMH 404

BLAST of MC08g2149 vs. TAIR 10
Match: AT5G57880.1 (multipolar spindle 1 )

HSP 1 Score: 352.4 bits (903), Expect = 4.7e-97
Identity = 192/396 (48.48%), Postives = 269/396 (67.93%), Query Frame = 0

Query: 16  DQSLKLAIAISLLRSKVIQKQQHTVSN---PPSDSDALRWKRKAKERKQELIRLREDLKQ 75
           ++SL+LAIA+SLLRSK    Q  + ++     S+SDALRWK+KAKERK+E+IRL+EDLK 
Sbjct: 14  EESLRLAIAVSLLRSKFQNHQSSSSTSRCYVSSESDALRWKQKAKERKKEIIRLQEDLKD 73

Query: 76  AEGLCVRISLSSFSLSLKIIVIRYDVFVLDPSHGDLFSQNATCKCFFFDNLGKFNPKQLE 135
           AE        SSF                   H DLF  NA+CKC+FFDNLG F+ +++ 
Sbjct: 74  AE--------SSF-------------------HRDLFPANASCKCYFFDNLGVFSGRRIG 133

Query: 136 DCTDNRFNDVLRRRFLR-------QVRYRERRRKSDSSINRKRLLDFNEVDEVEHLRASV 195
           + +++RFNDVLRRRFLR        +  R  RRK   S  R +  + +  +E EHLR S+
Sbjct: 134 EASESRFNDVLRRRFLRLACVVILSLARRRSRRKLTRSSQRLQPSEPDYEEEAEHLRISI 193

Query: 196 DFLVELCDTFSPVGEANFKNWAHQAVDFILASLKDLLPRKRSLEPIEGIISSLIMRLIRR 255
           DFL+EL +  S   ++NF NW+HQAVDFI ASLK L+   R+LE +E  IS +I +LI R
Sbjct: 194 DFLLELSEADS--NDSNFSNWSHQAVDFIFASLKKLISMGRNLESVEESISFMITQLITR 253

Query: 256 MCSPLK-DDSGDSDSDVRFYTQQLFRELGREPYIGQRAIISVSQRISAVGESFLFMDPFD 315
           MC+P+K ++    ++ V FY Q L R+LG EP+IGQRAI ++SQRIS + E+ LFMDPFD
Sbjct: 254 MCTPVKGNEVKQLETSVGFYVQHLIRKLGSEPFIGQRAIFAISQRISILAENLLFMDPFD 313

Query: 316 DNFPSLHECLFIMIQLVEFLLSDYLPMWSGDKNFDNLLLEELVISILHAKKALELLESRN 375
           ++FP + EC+FI+IQL+EFL+ DYL  W+ +  FDN++ EE + S++HA+KA++ LE RN
Sbjct: 314 ESFPEMDECMFILIQLIEFLICDYLLPWANEA-FDNVMFEEWIASVVHARKAVKALEERN 373

Query: 376 GVYVLYVDRVAGELANQISNIPRLRELKPDVLDDLI 401
           G+Y+LY+DRV GELA ++  I   RE++P +LD ++
Sbjct: 374 GLYLLYMDRVTGELAKRVGQITSFREVEPAILDKIL 379