MC08g1635 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC08g1635
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptiongibberellin 2-beta-dioxygenase 2-like
LocationMC08: 24244771 .. 24247862 (+)
RNA-Seq ExpressionMC08g1635
SyntenyMC08g1635
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGTAGCAGAAGCCAATTCTTCCCCAACCAGAAAGATGAGCAACCCCAGCGGAGCCATCCCCACCATTGATCTCTCCCTCTCCGCCACCGACCGCCGAGCCCTCTCCGGCCTCCTCGTCGAAGCCTGCGAAGACTATGGCATCTTCAAGCTCATCAACCACGCCGTCCCCCGGGGCGCCATTTCTAGAGTTGAAGAAGAAGTGGCTGCTTTCTTCGCCAAGCCCACCCCCGAGAAGCTCGCCGCCGCCCCTACAGATCGTCCTACCACCGGTGGCGGTGGCAAAACCACCCGCTTTGGCTACGGCGTCAAAAACATCGGCGCCAACGGCGACATGGGAGAGATTGAATATCTTCTCCTCTCCGCCAACCCCTCCTTGGTCTCTCAACTCGCCCACTCCATCTCCAATAACCCATCAAAATTCAGGTTTTTTTCGTTCTCTCCCTCCTCAAAACAACATATGAAACTATTAAAATATACCTAACTTATCGGCTAAGTTTGTATAGATTTTGTATGTCTAAAAAAAACATTTTTTAAAAAAAAAAACACGTGCTGGCGGAGAATCAAATTCATAGTATTAAGGTTATTTTTTTTTCTCTTTTTTATTTTTAGTATAACACATGGGGTGAGGATCGAACTCATAACAACACTCTTGGTAGGAGATATATGTTAATTAACGCTAAGCTATCTTCGCTTTGACGTTATTTTTTTTTCTCAACTCTCTCTTCCTGCGAGATTATTTAATTAAAATCTTAGGTATATCTTTGTGGAATTCTCCTCCGGAGTCAGATTTCTTGATTAAATGAGGGAATTGAAAAAATAGCACAAAAGTAAATATGTACTTATATTTAAAATGCTAAAAGTTTAAAATTTATTTTTTATTACAATATGTAGGGCGGGATCGAACTCACAATATCTTTTAATTAGAGATATATGTTAATTACGGATAAATTATGTTCGCTTCGGCTAAAAGCTTAAAATTTATTAAAATGATATAAATGGTAAAACTTATATCATCACACAAAACCAACTAAAAGTCTCTTTGGTAATATATATTTTATTTTATCTAATTTATTATAGTTTTTTTATTAAATTAATGTTAAACTCATGAATGAAGTTTAAATATTAATTTCTATAATCTTCACCCTTGTTTGAACTAAAAGAAGAAAAACTTAGTGATAATTTAAAACTTTTGGTTTATTTTCAATTTATTACTATTACTATTATTTGTTTGGAGAATTTAAAAAGGGAAAAATTTGGGAGTACTTCTCAAAGTTCCCAAACAGGCTCCGGAGTTGAAGCTGAAATTGAGGGTTGTTTGGTTCCTAAATATAATTATTTTAATGCTTCAATTTGACCAAGTTAGTTTTATTTCAAATTAAATATTATTATTTTGGTGGGAAATTTGTTTTTTTCCCCCTTTATCCTATAACCAAAAATGGTTCACATGAAAACCACAAATAAACAATTCAATATTTTACTAAATAAAATCTATACCCATCTCTTCTGTTTTCTTTCACATCTATATTTAATTATAAATTATTGACTTAACATCAAACTTGAAAAATAGCACACATGACTTTTACATTATGTTTGAACCGTTGATTTAAAAATTTCTATATAGGGTGGCTGAATTTCCTTTCAATAATTTATTGGAAGATAAACATTAGATTCATATTTTAAAATGCTCAGACGTTTATTAGTTAGCATATGTCTTATTGACTACCACCGTTAAAAAAATGAAATTTGTAACGGGTAACTACAATATTTGACAAATATAACAACTTTTGAGTTTTGTAAAAATGGCAAAATATTTGAAATTTGATTTTCAATTCTTAGTTATTCTGATTTTACCCTCTTCAAGTATATTAATGGTATATCAAATTTATATCGTTGTCTGGACTCCAATTTTTAGAAAATTGAAAAGTAGTTGCTACTTTTGTAAGGTCCTTCAAAATATGATAACTTTTCATTTTAAAAAAAAATCGAGAAATATTTCTTTTCCTACTTTTCTCTTTTCTACATGTCAAAATTTTAACGGTGGTTAACAGAAAAATAAGTGATTTTTTATACCAATGAAATTCCTCATAACTCTAAATATGGTCTAAATTGTGGTGCCAATAGGAATAAATCACCAATAGGGGGCAAAGCCTTTAAAACCTCACAAAAGTATCATAGAACTAAGTTGTTCATATCTTATATATTGTATATATTCTTTTTTTTTTCCCCCTTAAAAAGGATTGCTAGATCCATCTTTCTTGATCTTTTATTTTTCTTTTTGTTTTCCTTCTGGTCCTTTTAAGCTTTGGATTAGTGGGAGACGAGTAATAATAACATTCTCTAATGCACATTCCAACCTTGAAACTTTTGGTTTTTACCCTTTTATCAACTTTTTTTACCTTTTTTTTAACGCAGTTGTGCTGTGAATGATTACATGGAAGCAGTAAAAAATCTAGCCTGCGAGCTCCTGGATCTCCTAGCTGAGGGCTTGTGTATTCCAAACAAATCCATCTTCAGCCGTCTGATCTCAGACGTCCAAAATGACTCACTTTTCAGAATCAATCACTACCGTCAATCACCCGACTTCGATAACAATAATATCGGATTTGGAGAACACTCCGACCCTCAGATCATGACCATCTTGAGATCAAACGACGTCGATGGCCTCCAAATCTCATTGGACGATGGCTCCTGGATTCCTGTCTCTCCTGATCCCACTGCTTTCTATGTCATGATTGGTGATCTATTTCAGGTATATATATATATAATCACATCATTTTCTTTCTATTTAATATTATAGTTCAAATATTAATATAAATATAATTTTTTTTTTTTTTGGAAATGAAGGTTTTAACAAATGGAAGATTTGTGAGCATAAAGCATAGGGCAGTGGCAAACACAGGGCCAAAGCCAAGAATGTCAATGGCTTACTTTGGAGCACCATCTTTGGATGCATGGATCTCTCCTCTACCACAAATGGTAAACCCTAATCAACAAAGCCTTTATAGGCCTTTCACTTGGGCCCACTACAAGTCTCACATTTACTCTTTTCGTCTAGCTGGTTCAAACATCGACACCTTCAAGATCAAA

mRNA sequence

ATGGTGGTAGCAGAAGCCAATTCTTCCCCAACCAGAAAGATGAGCAACCCCAGCGGAGCCATCCCCACCATTGATCTCTCCCTCTCCGCCACCGACCGCCGAGCCCTCTCCGGCCTCCTCGTCGAAGCCTGCGAAGACTATGGCATCTTCAAGCTCATCAACCACGCCGTCCCCCGGGGCGCCATTTCTAGAGTTGAAGAAGAAGTGGCTGCTTTCTTCGCCAAGCCCACCCCCGAGAAGCTCGCCGCCGCCCCTACAGATCGTCCTACCACCGGTGGCGGTGGCAAAACCACCCGCTTTGGCTACGGCGTCAAAAACATCGGCGCCAACGGCGACATGGGAGAGATTGAATATCTTCTCCTCTCCGCCAACCCCTCCTTGGTCTCTCAACTCGCCCACTCCATCTCCAATAACCCATCAAAATTCAGTTGTGCTGTGAATGATTACATGGAAGCAGTAAAAAATCTAGCCTGCGAGCTCCTGGATCTCCTAGCTGAGGGCTTGTGTATTCCAAACAAATCCATCTTCAGCCGTCTGATCTCAGACGTCCAAAATGACTCACTTTTCAGAATCAATCACTACCGTCAATCACCCGACTTCGATAACAATAATATCGGATTTGGAGAACACTCCGACCCTCAGATCATGACCATCTTGAGATCAAACGACGTCGATGGCCTCCAAATCTCATTGGACGATGGCTCCTGGATTCCTGTCTCTCCTGATCCCACTGCTTTCTATGTCATGATTGGTGATCTATTTCAGGTTTTAACAAATGGAAGATTTGTGAGCATAAAGCATAGGGCAGTGGCAAACACAGGGCCAAAGCCAAGAATGTCAATGGCTTACTTTGGAGCACCATCTTTGGATGCATGGATCTCTCCTCTACCACAAATGGTAAACCCTAATCAACAAAGCCTTTATAGGCCTTTCACTTGGGCCCACTACAAGTCTCACATTTACTCTTTTCGTCTAGCTGGTTCAAACATCGACACCTTCAAGATCAAA

Coding sequence (CDS)

ATGGTGGTAGCAGAAGCCAATTCTTCCCCAACCAGAAAGATGAGCAACCCCAGCGGAGCCATCCCCACCATTGATCTCTCCCTCTCCGCCACCGACCGCCGAGCCCTCTCCGGCCTCCTCGTCGAAGCCTGCGAAGACTATGGCATCTTCAAGCTCATCAACCACGCCGTCCCCCGGGGCGCCATTTCTAGAGTTGAAGAAGAAGTGGCTGCTTTCTTCGCCAAGCCCACCCCCGAGAAGCTCGCCGCCGCCCCTACAGATCGTCCTACCACCGGTGGCGGTGGCAAAACCACCCGCTTTGGCTACGGCGTCAAAAACATCGGCGCCAACGGCGACATGGGAGAGATTGAATATCTTCTCCTCTCCGCCAACCCCTCCTTGGTCTCTCAACTCGCCCACTCCATCTCCAATAACCCATCAAAATTCAGTTGTGCTGTGAATGATTACATGGAAGCAGTAAAAAATCTAGCCTGCGAGCTCCTGGATCTCCTAGCTGAGGGCTTGTGTATTCCAAACAAATCCATCTTCAGCCGTCTGATCTCAGACGTCCAAAATGACTCACTTTTCAGAATCAATCACTACCGTCAATCACCCGACTTCGATAACAATAATATCGGATTTGGAGAACACTCCGACCCTCAGATCATGACCATCTTGAGATCAAACGACGTCGATGGCCTCCAAATCTCATTGGACGATGGCTCCTGGATTCCTGTCTCTCCTGATCCCACTGCTTTCTATGTCATGATTGGTGATCTATTTCAGGTTTTAACAAATGGAAGATTTGTGAGCATAAAGCATAGGGCAGTGGCAAACACAGGGCCAAAGCCAAGAATGTCAATGGCTTACTTTGGAGCACCATCTTTGGATGCATGGATCTCTCCTCTACCACAAATGGTAAACCCTAATCAACAAAGCCTTTATAGGCCTTTCACTTGGGCCCACTACAAGTCTCACATTTACTCTTTTCGTCTAGCTGGTTCAAACATCGACACCTTCAAGATCAAA

Protein sequence

MVVAEANSSPTRKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQNDSLFRINHYRQSPDFDNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVSPDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMVNPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK
Homology
BLAST of MC08g1635 vs. ExPASy Swiss-Prot
Match: Q9XHM5 (Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.4e-94
Identity = 184/349 (52.72%), Postives = 238/349 (68.19%), Query Frame = 0

Query: 2   VVAEANSSPTRKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGA 61
           +V  + +S  R     +  IPTIDLSL   +R  LS L+V+ACE+YG FK++NH+VP+  
Sbjct: 1   MVVPSPTSMIRTKKTKAVGIPTIDLSL---ERSQLSELVVKACEEYGFFKVVNHSVPKEV 60

Query: 62  ISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLL 121
           ISR++EE   FF+K + EK  A           G +T FGYG KNIG NGD GE+EYLLL
Sbjct: 61  ISRLDEEGIEFFSKNSSEKRQA-----------GTSTPFGYGCKNIGPNGDKGELEYLLL 120

Query: 122 SANPSLVSQLAHSIS-NNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLI 181
            +NP  +S+ + +I+ ++P KFSC VNDY++AVK+L CE+L+L AEGL +P+KS  S++I
Sbjct: 121 HSNPISISERSKTIAKDHPIKFSCIVNDYIKAVKDLTCEILELAAEGLWVPDKSSLSKII 180

Query: 182 SDVQNDSLFRINHY-------------RQSPDFDNNNIGFGEHSDPQIMTILRSNDVDGL 241
            D  +DSL RINHY              +  + +NNNIGFGEHSDPQI+TILRSN+V GL
Sbjct: 181 KDEHSDSLLRINHYPPVKKLGNDNWDPSKIQNSNNNNIGFGEHSDPQILTILRSNNVGGL 240

Query: 242 QISLDDGSWIPVSPDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAP 301
           QIS   G WIPV PDP+ FYVM+GD  QVLTNGRFVS++HR + NT  KPRMSM YF AP
Sbjct: 241 QISTHHGLWIPVPPDPSEFYVMVGDALQVLTNGRFVSVRHRVLTNT-TKPRMSMMYFAAP 300

Query: 302 SLDAWISPLPQMVNPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK 337
            L+  ISPL +MV  +   LYRPFTWA YK   Y+ RL  + +D FK++
Sbjct: 301 PLNWLISPLSKMVTAHSPCLYRPFTWAQYKQAAYALRLGDTRLDQFKVQ 334

BLAST of MC08g1635 vs. ExPASy Swiss-Prot
Match: Q9FZ21 (Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 6.3e-84
Identity = 166/326 (50.92%), Postives = 209/326 (64.11%), Query Frame = 0

Query: 12  RKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVEEEVAA 71
           + +S+P    P ID SL+  DR  LS  +V+ACE  G FK+INH V    I R E E   
Sbjct: 15  KTISSPEYNFPVIDFSLN--DRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGEE 74

Query: 72  FFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSANPSLVSQL 131
           FF KP  +KL A P            + FGYG KNIG NGD+GE+EYLLL ANP+ V+  
Sbjct: 75  FFNKPESDKLRAGP-----------ASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADK 134

Query: 132 AHSIS-NNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQNDSLFR 191
           + +IS ++P KFS A NDY+  V++LACE++DL  E L     S  S LI DV++DS+ R
Sbjct: 135 SETISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILR 194

Query: 192 INHYRQSPDFDN--NNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVSPDPTAFYV 251
           +NHY  +P   +    IGFGEHSDPQI+T+LRSNDVDGL+I   DG WIP+  DPT F+V
Sbjct: 195 LNHYPPAPYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFV 254

Query: 252 MIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMVNPNQQSLY 311
           ++GD  Q LTNGRF S++HR +ANT  KPRMS  YF AP L+A ISPLP+MV+P     Y
Sbjct: 255 LVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRY 314

Query: 312 RPFTWAHYKSHIYSFRLAGSNIDTFK 335
             FTW  YK   YS RL    ++ FK
Sbjct: 315 NSFTWGDYKKATYSLRLDVPRLEFFK 327

BLAST of MC08g1635 vs. ExPASy Swiss-Prot
Match: Q9C7Z1 (Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.1e-75
Identity = 162/327 (49.54%), Postives = 203/327 (62.08%), Query Frame = 0

Query: 21  IPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVEEEVAAFFAKPTPEK 80
           IP ID+S    +R  +S  +V+ACE  G FK+INH V +  ISR+E+E   FFAKP  EK
Sbjct: 15  IPIIDMS---QERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEK 74

Query: 81  LAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSAN-PSLVSQLAHSISNNP 140
            +  P ++P          F YG ++IG NGD GE+EYLL   N P+  SQL+       
Sbjct: 75  KSVRPVNQP----------FRYGFRDIGLNGDSGEVEYLLFHTNDPAFRSQLS------- 134

Query: 141 SKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQNDSLFRINHYRQSPD 200
             FS AVN Y+EAVK LA E+LDL AEGL +P  S FSRLIS V +DS+ R+NHY  S  
Sbjct: 135 --FSSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQ 194

Query: 201 F-----------DNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVSPDPTAFYV 260
           F               +GFGEH+DPQI+T+LRSN V GLQ+S  DG W+ VSPDP+AF V
Sbjct: 195 FFGEANLSDQSVSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCV 254

Query: 261 MIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMV-NPNQQSL 320
            +GDL QV+TNGRF+S++HRA+   G + R+S AYF  P L A I PL  MV   NQ  L
Sbjct: 255 NVGDLLQVMTNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRL 314

Query: 321 YRPFTWAHYKSHIYSFRLAGSNIDTFK 335
           Y+ FTW  YK   YS RL  S +D F+
Sbjct: 315 YQTFTWGEYKKRAYSLRLEDSRLDMFR 317

BLAST of MC08g1635 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.9e-74
Identity = 152/323 (47.06%), Postives = 205/323 (63.47%), Query Frame = 0

Query: 18  SGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVEEEVAAFFAKPT 77
           S +IP +DLS     +     L+V+ACED+G FK+INH +P  AIS++E E   FF+ P 
Sbjct: 24  SSSIPLVDLS-----KPDAKTLIVKACEDFGFFKVINHGIPLDAISQLESEAFKFFSLPQ 83

Query: 78  PEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSANPSLVSQLAHSIS- 137
            EK  A P +            FGYG K IG NGD+G IEYLLL+ N        H+ S 
Sbjct: 84  TEKEKAGPAN-----------PFGYGNKRIGLNGDIGWIEYLLLTTNQD------HNFSL 143

Query: 138 --NNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQNDSLFRINHY 197
              +  KF   + DY  A++N+ACE+LDL+AEGL I  K++FS+L+ D Q+D LFR+NHY
Sbjct: 144 YGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFRVNHY 203

Query: 198 RQSPDF---DNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVSPDPTAFYVMIG 257
              P+      N IGFGEH+DPQI++ILRSN+  G QISL DGSWI V PD ++F++ +G
Sbjct: 204 PACPELAINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSFFINVG 263

Query: 258 DLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMVNPNQQSLYRPF 317
           D  QV+TNGRF S++HR +AN G  PR+SM YF  P L   I+PLP ++   ++SLY+ F
Sbjct: 264 DSLQVMTNGRFKSVRHRVLAN-GIDPRLSMIYFCGPPLSEKIAPLPSLMK-GKESLYKEF 322

Query: 318 TWAHYKSHIYSFRLAGSNIDTFK 335
           TW  YKS  Y  RLA + +  ++
Sbjct: 324 TWFEYKSSTYGSRLADNRLGNYE 322

BLAST of MC08g1635 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-72
Identity = 142/318 (44.65%), Postives = 197/318 (61.95%), Query Frame = 0

Query: 21  IPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVEEEVAAFFAKPTPEK 80
           IP +DL+           L+V AC D+G FKL+NH VP   ++ +E E   FF K   EK
Sbjct: 27  IPVVDLT-----HPDAKNLIVNACRDFGFFKLVNHGVPLELMANLENEALRFFKKSQSEK 86

Query: 81  LAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSANPSLVSQLAHSI-SNNP 140
             A P D            FGYG K IG NGD+G +EYLLL+ NP ++S  +  I   NP
Sbjct: 87  DRAGPPD-----------PFGYGSKRIGPNGDVGWVEYLLLNTNPDVISPKSLCIFRENP 146

Query: 141 SKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQNDSLFRINHYRQSPD 200
             F   V +Y+ AVKN+   +L+L+AEGL I  ++  SRL+ D ++DS FR+NHY   P+
Sbjct: 147 HHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCFRLNHYPPCPE 206

Query: 201 ---FDNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVSPDPTAFYVMIGDLFQV 260
               + N +GFGEH+DPQI+++LRSN   GLQI L DG+W+ V PD T+F++ +GD  QV
Sbjct: 207 VQALNRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTSFFINVGDALQV 266

Query: 261 LTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMVNPNQQSLYRPFTWAHY 320
           +TNGRF S+KHR +A+T  K R+SM YFG P+L   I+PLP ++   ++ LY+ FTW  Y
Sbjct: 267 MTNGRFKSVKHRVLADT-TKSRLSMIYFGGPALSENIAPLPSVMLKGEECLYKEFTWCEY 326

Query: 321 KSHIYSFRLAGSNIDTFK 335
           K   Y+ RLA + +  F+
Sbjct: 327 KKAAYTSRLADNRLAPFQ 327

BLAST of MC08g1635 vs. NCBI nr
Match: XP_022157029.1 (gibberellin 2-beta-dioxygenase 2-like [Momordica charantia])

HSP 1 Score: 669 bits (1726), Expect = 1.91e-242
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MVVAEANSSPTRKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRG 60
           MVVAEANSSPTRKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRG
Sbjct: 1   MVVAEANSSPTRKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRG 60

Query: 61  AISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLL 120
           AISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLL
Sbjct: 61  AISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLL 120

Query: 121 LSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLI 180
           LSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLI
Sbjct: 121 LSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLI 180

Query: 181 SDVQNDSLFRINHYRQSPDFDNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVS 240
           SDVQNDSLFRINHYRQSPDFDNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVS
Sbjct: 181 SDVQNDSLFRINHYRQSPDFDNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVS 240

Query: 241 PDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMV 300
           PDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMV
Sbjct: 241 PDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMV 300

Query: 301 NPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK 336
           NPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK
Sbjct: 301 NPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK 336

BLAST of MC08g1635 vs. NCBI nr
Match: XP_022935060.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata])

HSP 1 Score: 444 bits (1142), Expect = 2.34e-153
Identity = 232/349 (66.48%), Postives = 268/349 (76.79%), Query Frame = 0

Query: 1   MVVAEANSSPTRKMSNPSGA---------IPTIDLSLSATDRRALSGLLVEACEDYGIFK 60
           MVVA  NSS T   S PS A         IPTIDLS   ++R+ALS LLVEA E YGIFK
Sbjct: 1   MVVASPNSSLTTN-SKPSAANTAAAAAARIPTIDLSCGTSNRQALSKLLVEASEKYGIFK 60

Query: 61  LINHAVPRGAISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANG 120
           LINH +P+  ISR EEE  AFFAKPT EKLAA+P   P   G   TTRFGYGV NIG +G
Sbjct: 61  LINHGIPQDCISRAEEEAMAFFAKPTAEKLAASPKSSPVDAG--NTTRFGYGVNNIGHHG 120

Query: 121 DMGEIEYLLLSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIP 180
           D+GEIEYLLLS+ PSL+SQL+ SIS NPSKFSCA+NDY++A KN+ACELLDL+AEGL I 
Sbjct: 121 DIGEIEYLLLSSQPSLISQLSFSISQNPSKFSCALNDYIQATKNVACELLDLMAEGLGIS 180

Query: 181 NKSIFSRLISDVQNDSLFRINHYRQSPDF----DNNNIGFGEHSDPQIMTILRSNDVDGL 240
           + SIFSR+I D ++DSL RINHYR  PDF     +N +GFGEHSDPQI  ILRSND+ GL
Sbjct: 181 DNSIFSRMILDSESDSLLRINHYRPLPDFIHPSPSNKLGFGEHSDPQIFAILRSNDIAGL 240

Query: 241 QISLDDGSWIPVSPDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAP 300
           QI LDDG WIPV PDPTA+YVMIGDLFQV TNGRF S+KHRA AN GP+ RMSMA+F +P
Sbjct: 241 QILLDDGLWIPVPPDPTAYYVMIGDLFQVFTNGRFGSLKHRATANIGPRSRMSMAFFASP 300

Query: 301 SLDAWISPLPQMVNPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK 336
           SLD WISPLP+MV  +Q  +YRPFTW  YK++IYS  LAGS +D+FKI+
Sbjct: 301 SLDTWISPLPEMVTSDQPCIYRPFTWREYKTYIYSLTLAGSRLDSFKIR 346

BLAST of MC08g1635 vs. NCBI nr
Match: KAG7018034.1 (Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 442 bits (1138), Expect = 9.83e-153
Identity = 230/350 (65.71%), Postives = 267/350 (76.29%), Query Frame = 0

Query: 1   MVVAEANSSPTRKMSNPSGA----------IPTIDLSLSATDRRALSGLLVEACEDYGIF 60
           MVVA  NSS T   S PS A          IPTIDLS   ++R+ALS LLVEA E YGIF
Sbjct: 1   MVVASPNSSLTTS-SKPSAADTATTTAAARIPTIDLSDGTSNRQALSKLLVEASEKYGIF 60

Query: 61  KLINHAVPRGAISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGAN 120
           KLINH +P+  ISR EEE  AFFAKPT EKLAA+P   P   G   TTRFGYG+ NIG +
Sbjct: 61  KLINHGIPQDCISRAEEEAMAFFAKPTAEKLAASPKSSPVDAG--NTTRFGYGINNIGRH 120

Query: 121 GDMGEIEYLLLSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCI 180
           GD+GEIEYLLLS+ PSL+SQLA S+S NPSKFSCA+NDY++A KN+ACELLDL+AEGL I
Sbjct: 121 GDIGEIEYLLLSSQPSLISQLAFSVSQNPSKFSCALNDYIQATKNVACELLDLMAEGLGI 180

Query: 181 PNKSIFSRLISDVQNDSLFRINHYRQSPDF----DNNNIGFGEHSDPQIMTILRSNDVDG 240
            + SIFSR+I D Q+DSL RINHYR  PDF     +N +GFGEHSDPQI  ILRSND+ G
Sbjct: 181 SDNSIFSRMILDSQSDSLLRINHYRPLPDFIHPSPSNKLGFGEHSDPQIFAILRSNDIAG 240

Query: 241 LQISLDDGSWIPVSPDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGA 300
           LQI LDDG W+PV PDPTA+YVMIGDLFQV TNGRF S+KHRA AN GP+ RMSM +F +
Sbjct: 241 LQILLDDGLWVPVPPDPTAYYVMIGDLFQVFTNGRFGSLKHRATANIGPRSRMSMTFFAS 300

Query: 301 PSLDAWISPLPQMVNPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK 336
           PSLD WISPLP+MV  +Q  +YRPFTW  YK++IYS  LAGS +D+FKI+
Sbjct: 301 PSLDTWISPLPEMVTADQPCIYRPFTWREYKTYIYSLTLAGSRLDSFKIR 347

BLAST of MC08g1635 vs. NCBI nr
Match: XP_038905831.1 (gibberellin 2-beta-dioxygenase 2-like [Benincasa hispida])

HSP 1 Score: 436 bits (1121), Expect = 3.05e-150
Identity = 229/343 (66.76%), Postives = 266/343 (77.55%), Query Frame = 0

Query: 1   MVVAEANSSPTRKMSNPSGA---IPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAV 60
           MVV   NSSPT    + + A   IPTIDLS   + R+ +S LLVEACE+YG FKLINH V
Sbjct: 1   MVVPPPNSSPTTDSKSSAVAAARIPTIDLSGITSKRQDISKLLVEACENYGFFKLINHGV 60

Query: 61  PRGAISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIE 120
           P+ +I+RVEEE  AFFA PT EKLAA P+   T      TTRFGYG+KNIG NGDMGE+E
Sbjct: 61  PQDSITRVEEEAFAFFAMPTAEKLAANPSPPATN-----TTRFGYGIKNIGCNGDMGELE 120

Query: 121 YLLLSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFS 180
           YLLLSA P+ VSQ A  +S NPS FSCA+N Y++ +KN+ACELLDL+AEGL I + SIFS
Sbjct: 121 YLLLSAQPTSVSQFALPLSKNPSNFSCALNGYIQEIKNVACELLDLMAEGLGISDTSIFS 180

Query: 181 RLISDVQNDSLFRINHYRQSPDFDN----NNIGFGEHSDPQIMTILRSNDVDGLQISLDD 240
           RLISD Q+DSLFRINHYR  PDF N    N IGFGEHSDPQI TILRSNDV GL+ISLDD
Sbjct: 181 RLISDDQSDSLFRINHYRPLPDFINLPPSNKIGFGEHSDPQIFTILRSNDVGGLEISLDD 240

Query: 241 GSWIPVSPDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWI 300
           G W+PVSPDPTA++++IGDLF VLTNGRF+S+ H+AVAN GPKPRMSMAYF +PSL+AWI
Sbjct: 241 GLWVPVSPDPTAYFIIIGDLFPVLTNGRFMSLTHKAVANGGPKPRMSMAYFASPSLNAWI 300

Query: 301 SPLPQMVNPNQQSLYRPFTWAHYKSHIYSFRL--AGSNIDTFK 334
           SPL +MV  NQ  LYRPFTW  YK+HIYS  L  A S +D+FK
Sbjct: 301 SPLQEMVTSNQPCLYRPFTWGEYKAHIYSIPLTTASSKLDSFK 338

BLAST of MC08g1635 vs. NCBI nr
Match: XP_023528042.1 (gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 435 bits (1119), Expect = 7.59e-150
Identity = 229/350 (65.43%), Postives = 267/350 (76.29%), Query Frame = 0

Query: 1   MVVAEANSSPTRKMSNPSGA----------IPTIDLSLSATDRRALSGLLVEACEDYGIF 60
           MVVA  NSS T   S PS A          IPTIDLS   ++R+ALS LLVEA E YGIF
Sbjct: 1   MVVASPNSSLTTS-SKPSAADTATTAAAARIPTIDLSGGTSNRQALSKLLVEASEKYGIF 60

Query: 61  KLINHAVPRGAISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGAN 120
           KLINH +P+  ISR EEE  AFFAKPT +KLAA+P  + +    G TTRFGYG+ NIG +
Sbjct: 61  KLINHGIPQDRISRAEEEAMAFFAKPTAQKLAASP--KSSLVDTGNTTRFGYGINNIGHH 120

Query: 121 GDMGEIEYLLLSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCI 180
           GD+GEIEYLLLS+ PSL+SQLA SIS NPSKFSCA+NDY++A KN+ACELLDL+AEGL I
Sbjct: 121 GDIGEIEYLLLSSQPSLISQLAFSISQNPSKFSCALNDYIQATKNVACELLDLMAEGLGI 180

Query: 181 PNKSIFSRLISDVQNDSLFRINHYRQSPDF----DNNNIGFGEHSDPQIMTILRSNDVDG 240
            + SIFSR+I D Q+DSL RINHYR  PDF     +N +GFGEHSDPQI  ILRSND+ G
Sbjct: 181 SDNSIFSRMILDSQSDSLLRINHYRPLPDFIHPSPSNKLGFGEHSDPQIFAILRSNDIAG 240

Query: 241 LQISLDDGSWIPVSPDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGA 300
           LQI LDDG W+PV PDPTA+YVMIGDLFQV TNGRF S+KHRA AN GP+ RMSM  F +
Sbjct: 241 LQILLDDGLWVPVPPDPTAYYVMIGDLFQVFTNGRFGSLKHRATANIGPRSRMSMTLFAS 300

Query: 301 PSLDAWISPLPQMVNPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK 336
           PSLD WISPLP+MV  +Q  +YRPFTW  YK++IYS  LAGS +D+FKI+
Sbjct: 301 PSLDTWISPLPEMVTSDQPCIYRPFTWREYKTYIYSLTLAGSRLDSFKIR 347

BLAST of MC08g1635 vs. ExPASy TrEMBL
Match: A0A6J1DVB7 (gibberellin 2-beta-dioxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111023854 PE=3 SV=1)

HSP 1 Score: 669 bits (1726), Expect = 9.25e-243
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MVVAEANSSPTRKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRG 60
           MVVAEANSSPTRKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRG
Sbjct: 1   MVVAEANSSPTRKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRG 60

Query: 61  AISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLL 120
           AISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLL
Sbjct: 61  AISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLL 120

Query: 121 LSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLI 180
           LSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLI
Sbjct: 121 LSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLI 180

Query: 181 SDVQNDSLFRINHYRQSPDFDNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVS 240
           SDVQNDSLFRINHYRQSPDFDNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVS
Sbjct: 181 SDVQNDSLFRINHYRQSPDFDNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVS 240

Query: 241 PDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMV 300
           PDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMV
Sbjct: 241 PDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMV 300

Query: 301 NPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK 336
           NPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK
Sbjct: 301 NPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK 336

BLAST of MC08g1635 vs. ExPASy TrEMBL
Match: A0A6J1F9H5 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111442043 PE=3 SV=1)

HSP 1 Score: 444 bits (1142), Expect = 1.13e-153
Identity = 232/349 (66.48%), Postives = 268/349 (76.79%), Query Frame = 0

Query: 1   MVVAEANSSPTRKMSNPSGA---------IPTIDLSLSATDRRALSGLLVEACEDYGIFK 60
           MVVA  NSS T   S PS A         IPTIDLS   ++R+ALS LLVEA E YGIFK
Sbjct: 1   MVVASPNSSLTTN-SKPSAANTAAAAAARIPTIDLSCGTSNRQALSKLLVEASEKYGIFK 60

Query: 61  LINHAVPRGAISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANG 120
           LINH +P+  ISR EEE  AFFAKPT EKLAA+P   P   G   TTRFGYGV NIG +G
Sbjct: 61  LINHGIPQDCISRAEEEAMAFFAKPTAEKLAASPKSSPVDAG--NTTRFGYGVNNIGHHG 120

Query: 121 DMGEIEYLLLSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIP 180
           D+GEIEYLLLS+ PSL+SQL+ SIS NPSKFSCA+NDY++A KN+ACELLDL+AEGL I 
Sbjct: 121 DIGEIEYLLLSSQPSLISQLSFSISQNPSKFSCALNDYIQATKNVACELLDLMAEGLGIS 180

Query: 181 NKSIFSRLISDVQNDSLFRINHYRQSPDF----DNNNIGFGEHSDPQIMTILRSNDVDGL 240
           + SIFSR+I D ++DSL RINHYR  PDF     +N +GFGEHSDPQI  ILRSND+ GL
Sbjct: 181 DNSIFSRMILDSESDSLLRINHYRPLPDFIHPSPSNKLGFGEHSDPQIFAILRSNDIAGL 240

Query: 241 QISLDDGSWIPVSPDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAP 300
           QI LDDG WIPV PDPTA+YVMIGDLFQV TNGRF S+KHRA AN GP+ RMSMA+F +P
Sbjct: 241 QILLDDGLWIPVPPDPTAYYVMIGDLFQVFTNGRFGSLKHRATANIGPRSRMSMAFFASP 300

Query: 301 SLDAWISPLPQMVNPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK 336
           SLD WISPLP+MV  +Q  +YRPFTW  YK++IYS  LAGS +D+FKI+
Sbjct: 301 SLDTWISPLPEMVTSDQPCIYRPFTWREYKTYIYSLTLAGSRLDSFKIR 346

BLAST of MC08g1635 vs. ExPASy TrEMBL
Match: A0A6J1J598 (gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111481875 PE=3 SV=1)

HSP 1 Score: 422 bits (1084), Expect = 4.86e-145
Identity = 221/338 (65.38%), Postives = 258/338 (76.33%), Query Frame = 0

Query: 1   MVVAEANSSPTRKMSNPSGA--IPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVP 60
           MVVA  NSS T   S PS A  IPTIDLS   ++R+AL  LLVEA E YG FKLINH +P
Sbjct: 1   MVVASPNSSLTTN-SKPSAAARIPTIDLSDRTSNRQALLKLLVEASEKYGFFKLINHGIP 60

Query: 61  RGAISRVEEEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEY 120
           +  ISR EEE  AFFAKPT EKL A+P   P   G   TTRFGYG+ NIG +GD+GEIEY
Sbjct: 61  QDCISRAEEEAMAFFAKPTAEKLPASPEISPIDVG--NTTRFGYGINNIGRHGDIGEIEY 120

Query: 121 LLLSANPSLVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSR 180
           LLL + PSL+SQLA SIS NPSKFSCA+NDY++A KN+ACELLDL+AEGL I + SIFSR
Sbjct: 121 LLLPSQPSLISQLAFSISQNPSKFSCALNDYIQATKNVACELLDLMAEGLGISDNSIFSR 180

Query: 181 LISDVQNDSLFRINHYRQSPDFDNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIP 240
           +I D Q+DSL RINHY   P   +N +GFGEHSDPQI  ILRSND+ GLQI LDDG W+ 
Sbjct: 181 MILDAQSDSLLRINHYFIHPT-PSNKLGFGEHSDPQIFAILRSNDIAGLQILLDDGLWVT 240

Query: 241 VSPDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQ 300
           V PDPTA+YVMIGDLFQV TNGRF S+KHRA AN GP+ RMSM +F +PSLD WISPLP+
Sbjct: 241 VPPDPTAYYVMIGDLFQVFTNGRFGSLKHRATANIGPRSRMSMTFFASPSLDTWISPLPE 300

Query: 301 MVNPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK 336
           M+  +Q  +YR FTW  YK++IYS  LAGS +D+FKI+
Sbjct: 301 MITSDQPCVYRSFTWREYKTYIYSLTLAGSRLDSFKIR 334

BLAST of MC08g1635 vs. ExPASy TrEMBL
Match: A0A5D3DA94 (Gibberellin 2-beta-dioxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00660 PE=3 SV=1)

HSP 1 Score: 398 bits (1022), Expect = 1.57e-135
Identity = 206/334 (61.68%), Postives = 248/334 (74.25%), Query Frame = 0

Query: 7   NSSPTRKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVE 66
           NS+ +   +  S  IPTIDLS   T+R+ALS L+VEACE YG FKLINH +P+ ++SRVE
Sbjct: 8   NSAKSSSAAAASTRIPTIDLSSITTNRQALSKLIVEACEKYGFFKLINHGIPQDSVSRVE 67

Query: 67  EEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSANPS 126
           EE  +FFA PT EKLA +  + P       TTR GYG++NIG +GDMGEIEYLLLSA PS
Sbjct: 68  EEAISFFAMPTAEKLATSSQNAPPIN---STTRIGYGIRNIGCSGDMGEIEYLLLSARPS 127

Query: 127 LVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQND 186
            VSQLA S+S NPS FS  +N Y++ +K++ACELLDL+AEGL I + SIFSR+I D  N 
Sbjct: 128 SVSQLALSLSKNPSNFSRVLNGYIQEIKDVACELLDLMAEGLGISDTSIFSRMILDDHNV 187

Query: 187 SLFRINHYRQSPDF-----DNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVSP 246
           SLFRIN YR  PD        N IGF EH+DPQI TILRSNDV G  IS++DGSW+PVSP
Sbjct: 188 SLFRINRYRPFPDDIINHPPPNTIGFSEHTDPQIFTILRSNDVGGFHISVNDGSWVPVSP 247

Query: 247 DPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMVN 306
           DPTA++++IGDLFQVLTNGRFV++KHR   N  PKPRMSMAYF APSL+A ISPL +MV 
Sbjct: 248 DPTAYFIIIGDLFQVLTNGRFVNVKHRVATNIEPKPRMSMAYFAAPSLNALISPLKEMVT 307

Query: 307 PNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKI 335
            NQ  LY+PFTW  Y++HIYS  LA S +D  KI
Sbjct: 308 SNQPCLYKPFTWGEYRAHIYSLPLASSKLDFVKI 338

BLAST of MC08g1635 vs. ExPASy TrEMBL
Match: A0A1S3BHH4 (gibberellin 2-beta-dioxygenase 2 OS=Cucumis melo OX=3656 GN=LOC103489905 PE=3 SV=1)

HSP 1 Score: 398 bits (1022), Expect = 1.57e-135
Identity = 206/334 (61.68%), Postives = 248/334 (74.25%), Query Frame = 0

Query: 7   NSSPTRKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVE 66
           NS+ +   +  S  IPTIDLS   T+R+ALS L+VEACE YG FKLINH +P+ ++SRVE
Sbjct: 8   NSAKSSSAAAASTRIPTIDLSSITTNRQALSKLIVEACEKYGFFKLINHGIPQDSVSRVE 67

Query: 67  EEVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSANPS 126
           EE  +FFA PT EKLA +  + P       TTR GYG++NIG +GDMGEIEYLLLSA PS
Sbjct: 68  EEAISFFAMPTAEKLATSSQNAPPIN---STTRIGYGIRNIGCSGDMGEIEYLLLSARPS 127

Query: 127 LVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQND 186
            VSQLA S+S NPS FS  +N Y++ +K++ACELLDL+AEGL I + SIFSR+I D  N 
Sbjct: 128 SVSQLALSLSKNPSNFSRVLNGYIQEIKDVACELLDLMAEGLGISDTSIFSRMILDDHNV 187

Query: 187 SLFRINHYRQSPDF-----DNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVSP 246
           SLFRIN YR  PD        N IGF EH+DPQI TILRSNDV G  IS++DGSW+PVSP
Sbjct: 188 SLFRINRYRPFPDDIINHPPPNTIGFSEHTDPQIFTILRSNDVGGFHISVNDGSWVPVSP 247

Query: 247 DPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMVN 306
           DPTA++++IGDLFQVLTNGRFV++KHR   N  PKPRMSMAYF APSL+A ISPL +MV 
Sbjct: 248 DPTAYFIIIGDLFQVLTNGRFVNVKHRVATNIEPKPRMSMAYFAAPSLNALISPLKEMVT 307

Query: 307 PNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKI 335
            NQ  LY+PFTW  Y++HIYS  LA S +D  KI
Sbjct: 308 SNQPCLYKPFTWGEYRAHIYSLPLASSKLDFVKI 338

BLAST of MC08g1635 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 312.4 bits (799), Expect = 4.5e-85
Identity = 166/326 (50.92%), Postives = 209/326 (64.11%), Query Frame = 0

Query: 12  RKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVEEEVAA 71
           + +S+P    P ID SL+  DR  LS  +V+ACE  G FK+INH V    I R E E   
Sbjct: 15  KTISSPEYNFPVIDFSLN--DRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGEE 74

Query: 72  FFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSANPSLVSQL 131
           FF KP  +KL A P            + FGYG KNIG NGD+GE+EYLLL ANP+ V+  
Sbjct: 75  FFNKPESDKLRAGP-----------ASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADK 134

Query: 132 AHSIS-NNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQNDSLFR 191
           + +IS ++P KFS A NDY+  V++LACE++DL  E L     S  S LI DV++DS+ R
Sbjct: 135 SETISHDDPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILR 194

Query: 192 INHYRQSPDFDN--NNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVSPDPTAFYV 251
           +NHY  +P   +    IGFGEHSDPQI+T+LRSNDVDGL+I   DG WIP+  DPT F+V
Sbjct: 195 LNHYPPAPYALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFV 254

Query: 252 MIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMVNPNQQSLY 311
           ++GD  Q LTNGRF S++HR +ANT  KPRMS  YF AP L+A ISPLP+MV+P     Y
Sbjct: 255 LVGDCLQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRY 314

Query: 312 RPFTWAHYKSHIYSFRLAGSNIDTFK 335
             FTW  YK   YS RL    ++ FK
Sbjct: 315 NSFTWGDYKKATYSLRLDVPRLEFFK 327

BLAST of MC08g1635 vs. TAIR 10
Match: AT1G47990.1 (gibberellin 2-oxidase 4 )

HSP 1 Score: 283.5 bits (724), Expect = 2.2e-76
Identity = 162/327 (49.54%), Postives = 203/327 (62.08%), Query Frame = 0

Query: 21  IPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVEEEVAAFFAKPTPEK 80
           IP ID+S    +R  +S  +V+ACE  G FK+INH V +  ISR+E+E   FFAKP  EK
Sbjct: 15  IPIIDMS---QERSQVSMQIVKACESLGFFKVINHGVDQTTISRMEQESINFFAKPAHEK 74

Query: 81  LAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSAN-PSLVSQLAHSISNNP 140
            +  P ++P          F YG ++IG NGD GE+EYLL   N P+  SQL+       
Sbjct: 75  KSVRPVNQP----------FRYGFRDIGLNGDSGEVEYLLFHTNDPAFRSQLS------- 134

Query: 141 SKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQNDSLFRINHYRQSPD 200
             FS AVN Y+EAVK LA E+LDL AEGL +P  S FSRLIS V +DS+ R+NHY  S  
Sbjct: 135 --FSSAVNCYIEAVKQLAREILDLTAEGLHVPPHS-FSRLISSVDSDSVLRVNHYPPSDQ 194

Query: 201 F-----------DNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVSPDPTAFYV 260
           F               +GFGEH+DPQI+T+LRSN V GLQ+S  DG W+ VSPDP+AF V
Sbjct: 195 FFGEANLSDQSVSLTRVGFGEHTDPQILTVLRSNGVGGLQVSNSDGMWVSVSPDPSAFCV 254

Query: 261 MIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMV-NPNQQSL 320
            +GDL QV+TNGRF+S++HRA+   G + R+S AYF  P L A I PL  MV   NQ  L
Sbjct: 255 NVGDLLQVMTNGRFISVRHRAL-TYGEESRLSTAYFAGPPLQAKIGPLSAMVMTMNQPRL 314

Query: 321 YRPFTWAHYKSHIYSFRLAGSNIDTFK 335
           Y+ FTW  YK   YS RL  S +D F+
Sbjct: 315 YQTFTWGEYKKRAYSLRLEDSRLDMFR 317

BLAST of MC08g1635 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 243.0 bits (619), Expect = 3.3e-64
Identity = 136/336 (40.48%), Postives = 195/336 (58.04%), Query Frame = 0

Query: 8   SSPTRKMSNPSGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVEE 67
           S P     +    IP ID+S   +        LV+ACED+G FK+INH V    +S +E 
Sbjct: 5   SKPVAIPKSGFSLIPVIDMSDPESKH-----ALVKACEDFGFFKVINHGVSAELVSVLEH 64

Query: 68  EVAAFFAKPTPEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSAN-PS 127
           E   FF+ P  EK   A               FGYG   IG NGD+G +EYLL++AN  S
Sbjct: 65  ETVDFFSLPKSEKTQVAGYP------------FGYGNSKIGRNGDVGWVEYLLMNANHDS 124

Query: 128 LVSQLAHSISNNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQND 187
               L  S+  +P  F  A+ +Y  +V+ +  ++L+ + +GL I  ++  S+L+SD   D
Sbjct: 125 GSGPLFPSLLKSPGTFRNALEEYTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTD 184

Query: 188 SLFRINHYRQSP------DFDNNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVS 247
           S+ R+NHY   P      +   N IGFGEH+DPQI+++LRSN+  GLQI+L+DGSWI V 
Sbjct: 185 SILRLNHYPPCPLSNKKTNGGKNVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVP 244

Query: 248 PDPTAFYVMIGDLFQVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMV 307
           PD T+F+  +GD  QV+TNGRF S++HR +AN   K R+SM YF  PSL   I+PL  ++
Sbjct: 245 PDHTSFFFNVGDSLQVMTNGRFKSVRHRVLANC-KKSRVSMIYFAGPSLTQRIAPLTCLI 304

Query: 308 NPNQQSLYRPFTWAHYKSHIYSFRLAGSNIDTFKIK 337
           +   + LY  FTW+ YK+  Y+ RL+ + +  F+ K
Sbjct: 305 DNEDERLYEEFTWSEYKNSTYNSRLSDNRLQQFERK 322

BLAST of MC08g1635 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 241.5 bits (615), Expect = 9.7e-64
Identity = 128/320 (40.00%), Postives = 194/320 (60.62%), Query Frame = 0

Query: 18  SGAIPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVEEEVAAFFAKPT 77
           S +IP ++L+      R     +V+ACE++G FK++NH V    ++R+E+E   FF  P 
Sbjct: 28  SHSIPVVNLADPEAKTR-----IVKACEEFGFFKVVNHGVRPELMTRLEQEAIGFFGLPQ 87

Query: 78  PEKLAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSANPSLVSQLAHSI-S 137
             K  A P +            +GYG K IG NGD+G IEYLLL+ANP L S    ++  
Sbjct: 88  SLKNRAGPPE-----------PYGYGNKRIGPNGDVGWIEYLLLNANPQLSSPKTSAVFR 147

Query: 138 NNPSKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQNDSLFRINHYRQ 197
             P  F  +V +YM+ +K ++ ++L+++AE L I  +   S+++ D ++DS  R+NHY  
Sbjct: 148 QTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKSDSCLRLNHYPA 207

Query: 198 SPDFDNN--NIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVSPDPTAFYVMIGDLF 257
           + +       +GFGEH+DPQI+++LRSN+  GLQI + DGSW+ V PD ++F++ +GD  
Sbjct: 208 AEEEAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPPDHSSFFINVGDAL 267

Query: 258 QVLTNGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMVNPNQQSLYRPFTWA 317
           QV+TNGRF S+KHR +A+T  + R+SM YFG P L   I+PLP +V      LY+ FTW+
Sbjct: 268 QVMTNGRFKSVKHRVLADT-RRSRISMIYFGGPPLSQKIAPLPCLVPEQDDWLYKEFTWS 327

Query: 318 HYKSHIYSFRLAGSNIDTFK 335
            YKS  Y  +L    +  F+
Sbjct: 328 QYKSSAYKSKLGDYRLGLFE 330

BLAST of MC08g1635 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 236.1 bits (601), Expect = 4.1e-62
Identity = 131/316 (41.46%), Postives = 190/316 (60.13%), Query Frame = 0

Query: 21  IPTIDLSLSATDRRALSGLLVEACEDYGIFKLINHAVPRGAISRVEEEVAAFFAKPTPEK 80
           IP IDL    TD  A +  +V+ACE++G FK+INH V    ++++E+E   FFA     K
Sbjct: 27  IPVIDL----TDSDAKT-QIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLK 86

Query: 81  LAAAPTDRPTTGGGGKTTRFGYGVKNIGANGDMGEIEYLLLSANPSLVSQLAHSI-SNNP 140
             A P D            FGYG K IG NGD+G +EY+LL+AN  L S    +I  + P
Sbjct: 87  DKAGPPD-----------PFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTP 146

Query: 141 SKFSCAVNDYMEAVKNLACELLDLLAEGLCIPNKSIFSRLISDVQNDSLFRINHYRQSPD 200
           + F  AV +Y++ +K ++ + L+++ E L I  K   SRL+   ++DS  R+NHY +  +
Sbjct: 147 AIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHYPEKEE 206

Query: 201 FD-NNNIGFGEHSDPQIMTILRSNDVDGLQISLDDGSWIPVSPDPTAFYVMIGDLFQVLT 260
                 IGFGEH+DPQ++++LRSND +GLQI + DG+W+ V+PD ++F+V++GD  QV+T
Sbjct: 207 TPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMT 266

Query: 261 NGRFVSIKHRAVANTGPKPRMSMAYFGAPSLDAWISPLPQMVNPNQQSLYRPFTWAHYKS 320
           NGRF S+KHR V NT  + R+SM YF  P L   I+PL  +V      LY  FTW+ YK 
Sbjct: 267 NGRFKSVKHRVVTNT-KRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKL 325

Query: 321 HIYSFRLAGSNIDTFK 335
             Y  +L    +  F+
Sbjct: 327 SAYKTKLGDYRLGLFE 325

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XHM51.4e-9452.72Gibberellin 2-beta-dioxygenase 2 OS=Pisum sativum OX=3888 GN=GA2OX2 PE=2 SV=1[more]
Q9FZ216.3e-8450.92Gibberellin 2-beta-dioxygenase 6 OS=Arabidopsis thaliana OX=3702 GN=GA2OX6 PE=2 ... [more]
Q9C7Z13.1e-7549.54Gibberellin 2-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA2OX4 PE=2 ... [more]
Q9SQ805.9e-7447.06Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1[more]
Q9XG831.1e-7244.65Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
XP_022157029.11.91e-242100.00gibberellin 2-beta-dioxygenase 2-like [Momordica charantia][more]
XP_022935060.12.34e-15366.48gibberellin 2-beta-dioxygenase 2-like [Cucurbita moschata][more]
KAG7018034.19.83e-15365.71Gibberellin 2-beta-dioxygenase 2, partial [Cucurbita argyrosperma subsp. argyros... [more]
XP_038905831.13.05e-15066.76gibberellin 2-beta-dioxygenase 2-like [Benincasa hispida][more]
XP_023528042.17.59e-15065.43gibberellin 2-beta-dioxygenase 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DVB79.25e-243100.00gibberellin 2-beta-dioxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC11102... [more]
A0A6J1F9H51.13e-15366.48gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111442... [more]
A0A6J1J5984.86e-14565.38gibberellin 2-beta-dioxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC11148187... [more]
A0A5D3DA941.57e-13561.68Gibberellin 2-beta-dioxygenase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BHH41.57e-13561.68gibberellin 2-beta-dioxygenase 2 OS=Cucumis melo OX=3656 GN=LOC103489905 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT1G02400.14.5e-8550.92gibberellin 2-oxidase 6 [more]
AT1G47990.12.2e-7649.54gibberellin 2-oxidase 4 [more]
AT1G78440.13.3e-6440.48Arabidopsis thaliana gibberellin 2-oxidase 1 [more]
AT1G30040.19.7e-6440.00gibberellin 2-oxidase [more]
AT2G34555.14.1e-6241.46gibberellin 2-oxidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 40..57
score: 36.36
coord: 210..231
score: 32.23
coord: 246..272
score: 26.94
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR47990:SF45GIBBERELLIN 2-BETA-DIOXYGENASE 6coord: 8..334
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 8..334
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 11..325
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..323
e-value: 2.2E-75
score: 256.0
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 186..285
e-value: 4.0E-23
score: 81.8
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 21..87
e-value: 8.5E-14
score: 52.3
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 184..287
score: 13.547062

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC08g1635.1MC08g1635.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045487 gibberellin catabolic process
biological_process GO:0009416 response to light stimulus
molecular_function GO:0052634 C-19 gibberellin 2-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding