MC04g1785 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g1785 vs. ExPASy Swiss-Prot
Match: D4AZ24 (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 3.3e-37
Identity = 190/725 (26.21%), Postives = 299/725 (41.24%), Query Frame = 0

Query: 29  SPPLSPPPPPAMSSPFEPLRQSPRPAAPVPFPQTQSTVLPDPSDFFSPHL--LSSPLPTN 88
           S P +PP   A    F+P+ + P PA         +    D     + H+   + P+ TN
Sbjct: 179 SQPSAPPATMAGQDIFQPIAKDPIPA---------NIKSRDDHPVKANHIENPTGPISTN 238

Query: 89  SFFQNFTVKNGDQPEYIHPYLIKSALSSIAISYPSIVCNSAVGFQVFNADLTVSAAGNSG 148
            F+ NF + N     + HPY +  A      S   +  +     Q    +      GN  
Sbjct: 239 KFYANFFLGNQTSTTFTHPYTMIWAKGDKNASSFGMAISHVEPSQRATGEPNNKLPGNPV 298

Query: 149 SF-----SQKSHIISEFSDLSVTLDIPSANLRSF-------------------LVRGSPF 208
            +       KS ++S  S+L  +  +  A  ++F                   LV+G  F
Sbjct: 299 RYYINPVGIKSLVLSA-SELKESTTMSVAKPQAFSAQAILRPTGGSSESITFPLVQGMGF 358

Query: 209 LTFEVFNNTPISISTIHAIL------SFSSNSSFTKFTVTLNNNQTWLIY----SSSPIN 268
           +T  ++NN   +I +  A+L      + S      K+ +TL +++ WL+Y    + +   
Sbjct: 359 IT-AIYNNLQPAIQS--AVLFRKVEPAGSPQGGIFKYKITLEDDKNWLLYVIPENGADPK 418

Query: 269 FTHDLSKITSG--GFSGIIRIAAVPD-PHCESILDRFSSCY----PVSGEAKFGNPFCLE 328
              + +K+ SG  GF G+I++A  P     E I D+ +  Y     +SG          +
Sbjct: 419 LKLEGNKLISGPTGFKGVIQVAKNPSAEEGEGIYDKSAGSYATDIKISGSVGTDGTGTYK 478

Query: 329 YKWEKRGWG-DLLMLAHPLHLRLLSGGGAVLDDLKYKSID----GDLVGIVGSSWVL--- 388
           + +EK G G  L+M A P H+   S   A  +  K   +     G     VG SW +   
Sbjct: 479 FSFEKAGKGAPLVMYALPHHVE--SFDDATKNTKKNMKLSTTTKGMATACVGDSWTMVEG 538

Query: 389 --------------QSDPVSVTWHSINGVGAEFHSEIISALVKDVEGLNSAPITTTSSYF 448
                          S  V+++  + N + A   +E    L +D+E          S YF
Sbjct: 539 NLPLSMDFAPWKPGSSSQVTLSEGAKNAIKAVAGNE----LSQDME----LQTNLNSMYF 598

Query: 449 YGKSIAR-AARLALIAEEVNFLQVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVT 508
            GK + + A  +  + E V            LK + + ++         +YD  W G V+
Sbjct: 599 SGKGLNKFAGAIYTVQELVGDKAAASGPLNSLKESFKRFVDNK-QQIPLVYDNVWKGVVS 658

Query: 509 Q--QGSSDSGADFGFGVYNDHHFHIGYFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNL 568
                  D+G DFG  +YNDHHFH GYF+   A+L K+DPAW    +     L+ D  N 
Sbjct: 659 SGTYEKGDTGLDFGNTLYNDHHFHYGYFILTAAILGKLDPAWLDANKAYVNMLVRDSGN- 718

Query: 569 SRRPNSSFPRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHL 628
           S   +  FP  R FD Y  HSWA GL E +DG++QESTSE     Y+  + G   GD  +
Sbjct: 719 SVDNDEHFPFSRAFDWYHGHSWAKGLFESSDGKDQESTSEDTMYAYAIKMWGKTSGDKSM 778

Query: 629 ASIGSTLTAMEIKAAQTWWQIREGDNLYEVDFERENKVVGVLWSNKRDSGLWFAPPDWRE 681
            + G+ +  +  +    ++ + + DN+ +      NKV G+L+ NK D   +F      E
Sbjct: 779 EARGNLMLGILARTLNNYF-LMKNDNVNQPKNFIGNKVTGILFENKIDHTTYFGAN--LE 838

BLAST of MC04g1785 vs. ExPASy Swiss-Prot
Match: Q9UT45 (Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 3.3e-37
Identity = 168/642 (26.17%), Postives = 271/642 (42.21%), Query Frame = 0

Query: 79  LSSPLPTNSFFQNFTVKNGDQPEYIHPYLI----KSALSSIAISYPSIVCNSAVGFQVFN 138
           LSSP+ TN FF N  + +     Y  P+         +  I I++      +    +V +
Sbjct: 78  LSSPIETNKFFDNNLLGSRTNFMYADPFRYWWQSSDTMGGICIAH------TDDNQRVMD 137

Query: 139 ADLTVSA------------AGNSGSFSQKSHIISEFSDLSV--TLDIPSANLRSFLVRGS 198
            D T+ +             G SG  S    I+ E   +S   T    S++++  L  G 
Sbjct: 138 TDDTIPSYYYEPIGICSLGFGASGITSNTDPIVDEIDQMSARFTFSWDSSSMQLTLTEGM 197

Query: 199 PFLTFEVFNNTPISIST---IHAILSFSSNSSFTKFTVTLNNNQTWLIY-SSSPINFTHD 258
              T    N  P   S+   I+  +     S+  K+ VT+++N  WLIY     +  T  
Sbjct: 198 AVTTAVYTNAIPQIFSSTLYINDFVEVPGTSAVQKYRVTMSDNHVWLIYIYGDSLTLTES 257

Query: 259 LSKITSGG--FSGIIRIAAVP--DPHCESILDRFSSCY----PVSGEAKFGNPFCLEYKW 318
            S++  G   F+G I+IA +P  D   E++ D ++  Y     +SG  +    +   Y +
Sbjct: 258 TSQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTYAGVYITGISISGYVEDAVGY---YSF 317

Query: 319 EKRGWGDL----LMLAHPLHLRLLSGGGAVLDDLKYKSIDGDLVGIVGSSWVL-QSDPVS 378
           +    GD     L    P  +     G  V   +    + GD+    G+S    ++ P  
Sbjct: 318 DFTTAGDTSVEPLFFLLPHQVDTAVSGTKVTSIVLASLVSGDMNAAAGNSITFAEAIPQD 377

Query: 379 VTWHSINGVGAEF-HSEIISALVKDVEGLN-----SAPITTTSSYFYGKSIARAARLA-- 438
           + +   +  G +  +SE    ++ +V G       SA     S Y+ GK +A+ A L   
Sbjct: 378 IGFLPWSPTGGQIGYSEEALEIIAEVAGTELGEDFSANSNLNSMYYSGKVLAKYAMLCVT 437

Query: 439 ---LIAEEVNFLQVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQG-SSDSGA 498
              ++ +E +  Q I ++       ++             YD  W G V+  G S DS A
Sbjct: 438 INDILGDETSSEQCIQKLEAAFARFVD-----NQQIYPLTYDNTWKGVVSVAGLSGDSLA 497

Query: 499 DFGFGVYNDHHFHIGYFLYAIAVLVKIDPAWGRKFRPQAY--SLMADFMNLSRRPNSSFP 558
           DFG   YNDHHFH GYF++  AV+  IDP W      + +   L+ D  N S   +  FP
Sbjct: 498 DFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTGNNKEWVNFLVRDVANPSSN-DPYFP 557

Query: 559 RLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTA 618
           + R  D+Y  H WASGL E  DG+++ESTSE  N ++   L G   GD+ +    + +  
Sbjct: 558 KHRMIDIYHGHGWASGLFESNDGKDEESTSEDYNFFFGMKLWGQVIGDSDMEDRANIILG 617

Query: 619 MEIKAAQTWWQIREGDNLYEVDFERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP 671
           +E  A   +    +G+   +    + N V G+ + NK     +F      E   GI +LP
Sbjct: 618 IERNALNKYMLYADGN--VQPTSMQPNYVAGITFMNKITHTTYFGTN--IEYIQGIHMLP 677

BLAST of MC04g1785 vs. ExPASy Swiss-Prot
Match: Q09850 (Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 1.3e-33
Identity = 168/660 (25.45%), Postives = 281/660 (42.58%), Query Frame = 0

Query: 82  PLPTNSFFQNFTVKNGDQPEYIHPYLIK----SALSSIAISY------------PSIVCN 141
           P+ TN F+ N  +     P + HPY +     S+   +AIS+             S+ C 
Sbjct: 34  PIQTNKFYGNLYIGTRHNPSWSHPYSVTWLNGSSYYGLAISHIDDSQRVFGPDPESVPCQ 93

Query: 142 ---SAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPSANLRSFLVRGSPF 201
              +  G           A+GN  S  Q  H  S  + LS T    S  +   +V G  F
Sbjct: 94  YYFNPAGLYSIIISAREFASGNLLSLDQSRH-FSIQATLSATTS-GSGTIILPIVAGMGF 153

Query: 202 LTFEVFNNTPISISTIHAILSFSSNSSFT---KFTVTLNNNQTWLIY-----SSSPINFT 261
           ++    N TP+  S+I  + S  +  +F+   K+ + L + + W +Y     SSS  N T
Sbjct: 154 VSGYYTNLTPVFNSSI--LFSSITKINFSRGYKYRIQLTDGKIWFLYAFPTSSSSTFNLT 213

Query: 262 --HDLSKITSGGFSGIIRIAAVP-----DPHCESILDRFSSCYPVS---GEAKFGNPFCL 321
              +    TS  F+G+I+I  VP     + + ++I D  +  Y  S        G     
Sbjct: 214 LASNSQLTTSTKFTGLIQICKVPNESVNNSYPDTIYDASAGVYTTSISLSAQVSGTTGEY 273

Query: 322 EYKWEKRGWGDL--LMLAHPLHLRLLSGGGAVLDDLKYKSIDGDLVGIVGSSWVLQSDPV 381
            +++   G+ +L  LM A P H++         D   YK+     +G+  ++  +     
Sbjct: 274 WFRFATAGYTNLNPLMFALPHHMQSFGS-----DTQAYKT----GLGLASTTMGIMFAYA 333

Query: 382 SVTWHSI---------------NGVGAEFHSEIISALVK-------DVEGLNSAPITTTS 441
           + TWH I               NG G+  +S    A ++       + + +N++ +   S
Sbjct: 334 TKTWHLIEKNLPTQVGFLPIPWNG-GSNTYSPTALAAIRAACATDINFDVVNASNL--DS 393

Query: 442 SYFYGKSIARAARLALIAEEVNFLQVIPEVRKFLKGAIEPWLRGTFNGNGF--LYDGKWG 501
            Y  GK +A  A++ L+A  +  L         L    +   R T N   +  +YD  + 
Sbjct: 394 MYTSGKIVAMYAQVCLVASRI--LGDSTLTNTGLTKLKQAMARFTTNTQMYPLVYDTTYK 453

Query: 502 GFVTQQGSSDSGADFGFGVYNDHHFHIGYFLYAIAVLVKIDPAW--GRKFRPQAYSLMAD 561
           G ++  G S   AD+G   YNDHHFH GY +YA AV+  +DP+W      R    +L+ D
Sbjct: 454 GIISTAGYSSPLADYGNTYYNDHHFHWGYHIYACAVIGLLDPSWLVNDNIR-YVNALLRD 513

Query: 562 FMNLSRRPNSSFPRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYG 621
             N S   ++ F   R FD +  HSWA+G+ E  DG+++ESTSE  N  Y+  L G+   
Sbjct: 514 SANPS-ESDTYFAMFRNFDWFVGHSWATGIFESGDGKDEESTSEDFNFLYATKLWGMVRN 573

Query: 622 DTHLASIGSTLTAMEIKAAQTWWQIREGDNLYEVDFERENKVVGVLWSNKRDSGLWFAPP 677
           DT L +  + + A+   +  T+  +    ++        N V G+ + NK D   +F+  
Sbjct: 574 DTVLINRANLMLAVLKNSLNTYIYMTPTTSVQPSQI-LGNYVTGITFMNKVDYATYFSAE 633

BLAST of MC04g1785 vs. ExPASy Swiss-Prot
Match: P53753 (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.7e-33
Identity = 173/681 (25.40%), Postives = 286/681 (42.00%), Query Frame = 0

Query: 60   PQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSALSS---IA 119
            P T     P+P           P+ TN F+ N  V + + P +++PY +    SS    A
Sbjct: 411  PPTVFDRSPNPMSLADGVSNDGPIQTNKFYTNLIVGSQESPAFVYPYSLWKYTSSSYGFA 470

Query: 120  ISYPSIVCNSAVG--------------------FQVFNADLTVSAAGNSGSFSQKSHIIS 179
            + + ++   S  G                    F   N D +++   +  + S    ++S
Sbjct: 471  VQHTTVDQYSYGGYDSSGNAEYLVNPLGIAHVVFSASNFDSSMTMQVDEMTLSSTRVVLS 530

Query: 180  EFSDLSVTLDIPSANLRSFLVRGSPFLT--FEVFNNTPISIST-IHAILSFSSNS---SF 239
            E +D S  L+IP       LV+G  F T  +    N  I  S   + I+S SS++     
Sbjct: 531  ESNDSSNYLEIP-------LVQGMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQGI 590

Query: 240  TKFTVTLNNNQTWLIYSSSPINFTH-DLS---------KITSGGFSGIIRIAAVP-DPHC 299
             K+ +TL N  TWL Y   P + T  D S         K ++     II++A  P +   
Sbjct: 591  LKYRITLLNGVTWLCYVIGPDDLTSTDFSLEVSSEYEIKASASVDGLIIQLAVAPSETDY 650

Query: 300  ESILDRFSSCYPVSGE---AKFGNPFCLEYKWEKRG---WGDLLMLAHPLHLRLLSG--- 359
            E   D+ +  Y  + +      G+    E+ +  +G    G  ++ A P H    S    
Sbjct: 651  EVFYDQAAGMYVTNFKLQGVSDGSTATYEFSYTTQGESASGSTMIFALPHHESSFSDIMQ 710

Query: 360  ---GGAVLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTWHSINGVG-AEFHSEIISALVK 419
                G  L       ++G L   +  S  L      + W S  G    E+  E +  L +
Sbjct: 711  DYYTGIQLASTTKGVMNGYLTTSLQFSTSLNRQISWLPWSSQLGSNLLEYSKEQLQLLAE 770

Query: 420  ----DVEGLNSAPITTTSSYFYGKSIARAARLAL-IAEEVNFLQVIPEVRKFLKGAIEPW 479
                +++   S  I+  ++Y+ GK I + + + L ++E +          + +K A +  
Sbjct: 771  VANSELQVSISESISGLNTYYLGKVIDKYSYILLTVSEIIQDEASTKSTLENIKSAFDIL 830

Query: 480  LRGTFNGNGFLYDGKWGGFVT--QQGSSDSGADFGFGVYNDHHFHIGYFLYAIAVL---- 539
            L+        +YD K+ G V+    GS+ +  DFG   YNDHHFH GY ++A AV+    
Sbjct: 831  LQNE-QTYPLIYDTKFNGLVSSGDWGSTSTQYDFGNTYYNDHHFHYGYIIHAAAVIGYVD 890

Query: 540  VKIDPAWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASGLTEFADGRNQ 599
             K++  W    +    SL+ D  N S + +  F + R FD +  HSWA+GL E  +G+N+
Sbjct: 891  SKLNGTWAADNKDWVNSLVRDVANPSEK-DEYFAQSRMFDWFNGHSWAAGLYENGNGKNE 950

Query: 600  ESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDNLYEVDFERE 659
            ES+SE  N  Y+  L G   GD  +   G  + ++ +K A   +   + DN  E +    
Sbjct: 951  ESSSEDYNFAYAMKLWGATIGDQSMELRGDLMISI-MKDAMNDYFYYQNDNTVEPEEIIG 1010

Query: 660  NKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFARELVEWTLPSL 677
            NKV G+L+ N  D   +F      E   GI +LP+ P++  + S+     E      P +
Sbjct: 1011 NKVSGILFDNIIDYTTYFGTN--TEYIHGIHMLPITPVSSNIRSETFVEEEWQTKIEPII 1070

BLAST of MC04g1785 vs. ExPASy Swiss-Prot
Match: Q12168 (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 7.3e-29
Identity = 176/703 (25.04%), Postives = 297/703 (42.25%), Query Frame = 0

Query: 27  FQSPPLSPPPPPAMSSPFEPLRQSPRPAAPVPFPQTQSTVLPDPSDFFSPHLLSSPLPTN 86
           FQSP LS    P   S F  ++       PVP P      + D S          PL TN
Sbjct: 76  FQSPVLSNLKAP--PSVFNKVQH------PVPKPN-----IDDQS--------VDPLETN 135

Query: 87  SFFQNFTVKNGDQPEYIHPYLI----------KSALSSIAISYPSIVCNSAVGFQVFNAD 146
            F+ N  + +  QP + HPY I           +A  ++A         +   F  FN  
Sbjct: 136 KFYTNMLLDDNTQPIWTHPYSIWFSRDPELFGLAANHTLASQRVFDTTTNPPRF-YFNPT 195

Query: 147 LTVSAAGNSGSFSQKSHIISEFSDLS-----VTLDIPSANLRSF-LVRGSPFLTFEVFNN 206
              S    +  F   + I  EF D+      + + + S+    F LV+G  F+T  ++++
Sbjct: 196 NIKSFVFKAREFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQGMGFVT-AIYHD 255

Query: 207 TPISIST---IHAILSFSSNSSFTKFTVTLNNNQTWLIYSSSP---------INFTHDLS 266
               + +     ++   S N  + K+ + L NN+ W++Y +SP         I+     +
Sbjct: 256 LGFELRSAVGFRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQDFQISLLDSNT 315

Query: 267 KITSGGFSGII-RIAAVPDPHCESILDRFSSCYPV----SGEAKFGNPFCLEYKWEKRGW 326
            I+S   +G+I +++A   P     +D  + CYPV    SG+    +     + +   G+
Sbjct: 316 IISSHKINGLICQLSADSVPS----IDMAAGCYPVYCDLSGQTVDEHFTNYRFNYTVAGY 375

Query: 327 ---GDLLMLAHPLHLRLLSGG------GAVLDDLKYKSIDGDLVGIVGSSWVLQ------ 386
              G  LM A P H    +         + LD     ++ G + G + +S+ +Q      
Sbjct: 376 SQSGTTLMYALPHHKAAFTPEMQEREIASSLD----STVKGLMTGYLTNSFDMQVQVPQE 435

Query: 387 --SDPVSVTWHSINGVGAEFHSEIISALVKDVEGLNSAPITTTSS-YFYGKSIARAARLA 446
              +PV+++ +       E  S+I  A V++V+  +    +   S YF GK +A+ A + 
Sbjct: 436 LGFEPVALSLNKKADYSQEKLSKIREAAVQEVQLSDPQQESNIDSMYFSGKILAKYAWIL 495

Query: 447 LIAEEV-NFLQVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFG 506
            +   + +   +  E+   L  A+E ++          YD  W G ++   S  S  DFG
Sbjct: 496 YVTHYILHDENLTKELLSKLTIAMERFISNQ-QVLPLNYDVSWKGIIS---SGSSSQDFG 555

Query: 507 FGVYNDHHFHIGYFLYAIAVLVKIDP--------AWGRKFRPQAYSLMADFMNLSRRPNS 566
              YNDHHFH  Y +   A++  +D         +W    R     L+ D+  +    + 
Sbjct: 556 NSYYNDHHFHYSYHVITAAIISLVDSDLSGVTNNSWLENNRDWVECLIRDYSGVD-NDDP 615

Query: 567 SFPRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGST 626
            FP+ R FD +  HSWA GL    DG+++ESTSE VN+ Y+  L GL  G++ L  I + 
Sbjct: 616 YFPQFRSFDWFNGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLVTGNSKLTDIANL 675

Query: 627 LTAMEIKAAQTWWQIREGDNLYEVDFERENKVVGVLWSNKRDSGLWFA-PPDWRECRLGI 667
              +     Q+++ + E +N  +      NKV G+L+ NK D   +F   P +      I
Sbjct: 676 QLGIMRNVFQSYF-LYESNNTVQPKEFIGNKVSGILFENKIDHATYFGMEPQY------I 734

BLAST of MC04g1785 vs. NCBI nr
Match: XP_022134434.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Momordica charantia])

HSP 1 Score: 1467 bits (3797), Expect = 0.0
Identity = 723/723 (100.00%), Postives = 723/723 (100.00%), Query Frame = 0

Query: 1   MLKKLRRNFKRFKTLINKSFSSYKFPFQSPPLSPPPPPAMSSPFEPLRQSPRPAAPVPFP 60
           MLKKLRRNFKRFKTLINKSFSSYKFPFQSPPLSPPPPPAMSSPFEPLRQSPRPAAPVPFP
Sbjct: 1   MLKKLRRNFKRFKTLINKSFSSYKFPFQSPPLSPPPPPAMSSPFEPLRQSPRPAAPVPFP 60

Query: 61  QTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSALSSIAISYP 120
           QTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSALSSIAISYP
Sbjct: 61  QTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSALSSIAISYP 120

Query: 121 SIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPSANLRSFLVRGS 180
           SIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPSANLRSFLVRGS
Sbjct: 121 SIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPSANLRSFLVRGS 180

Query: 181 PFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSSPINFTHDLSKI 240
           PFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSSPINFTHDLSKI
Sbjct: 181 PFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSSPINFTHDLSKI 240

Query: 241 TSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWEKRGWGDLLMLA 300
           TSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWEKRGWGDLLMLA
Sbjct: 241 TSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWEKRGWGDLLMLA 300

Query: 301 HPLHLRLLSGGGAVLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTWHSINGVGAEFHSEI 360
           HPLHLRLLSGGGAVLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTWHSINGVGAEFHSEI
Sbjct: 301 HPLHLRLLSGGGAVLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTWHSINGVGAEFHSEI 360

Query: 361 ISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPEVRKFLKGAIEP 420
           ISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPEVRKFLKGAIEP
Sbjct: 361 ISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPEVRKFLKGAIEP 420

Query: 421 WLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHIGYFLYAIAVLVKIDP 480
           WLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHIGYFLYAIAVLVKIDP
Sbjct: 421 WLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHIGYFLYAIAVLVKIDP 480

Query: 481 AWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASGLTEFADGRNQESTSE 540
           AWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASGLTEFADGRNQESTSE
Sbjct: 481 AWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASGLTEFADGRNQESTSE 540

Query: 541 AVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDNLYEVDFERENKVVG 600
           AVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDNLYEVDFERENKVVG
Sbjct: 541 AVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDNLYEVDFERENKVVG 600

Query: 601 VLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFARELVEWTLPSLGREGV 660
           VLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFARELVEWTLPSLGREGV
Sbjct: 601 VLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFARELVEWTLPSLGREGV 660

Query: 661 GEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHSRGGGEGSGGGWRHWW 720
           GEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHSRGGGEGSGGGWRHWW
Sbjct: 661 GEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHSRGGGEGSGGGWRHWW 720

Query: 721 LSH 723
           LSH
Sbjct: 721 LSH 723

BLAST of MC04g1785 vs. NCBI nr
Match: XP_038890545.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Benincasa hispida])

HSP 1 Score: 1258 bits (3255), Expect = 0.0
Identity = 625/742 (84.23%), Postives = 662/742 (89.22%), Query Frame = 0

Query: 1   MLKKLRRNFKRFKTLINK-----------SFSSYKFPFQSPPLSPPPPPAMSSPFEPLRQ 60
           MLKKL+RNFK+FKTL NK           SF  YKFP QSPPLSP PPP MSSP +P  Q
Sbjct: 1   MLKKLKRNFKKFKTLTNKRFNYSSSSSSSSFKRYKFPSQSPPLSPRPPPEMSSPTQPFWQ 60

Query: 61  SPRPAAPVPFPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK 120
           SP P A + FPQ QSTVLPDPS FF+PHLLSSPLPTNSFFQNFTVKNGDQPEY HPYLIK
Sbjct: 61  SPPPVAAL-FPQIQSTVLPDPSKFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYFHPYLIK 120

Query: 121 SALSSIAISYPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPS 180
           S+LS+++ISYPS+  NSA G+QVFNAD TVS   N  S SQK HIIS FSDLS+TLDIPS
Sbjct: 121 SSLSTVSISYPSMFLNSAFGYQVFNADWTVSE--NPSSVSQKPHIISSFSDLSLTLDIPS 180

Query: 181 ANLRSFLVRGSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSS 240
            NLR FLVRGSPF+TFEVFNNTPISISTIHAILSFSSNSS TKFTV LNNNQTWLIYSSS
Sbjct: 181 GNLRFFLVRGSPFMTFEVFNNTPISISTIHAILSFSSNSSLTKFTVALNNNQTWLIYSSS 240

Query: 241 PINFTHDLSKITSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWE 300
           PIN TH LS+ITSGGFSGI+RIA +P+PHCE+ILDRFSSCYPVSGE  F NPF LEYKWE
Sbjct: 241 PINLTHSLSQITSGGFSGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYKWE 300

Query: 301 KRGWGDLLMLAHPLHLRLLSG---GGAVLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTW 360
           K GWG+LLMLAHPLHLRLLSG   G  VLDD KYKSIDGDLVG+VG SWVL+ DPVSVTW
Sbjct: 301 KNGWGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGVVGGSWVLKPDPVSVTW 360

Query: 361 HSINGVGAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQV 420
           HSINGVG EF SEII+ALVKDVEGL S+PITTTSSYFYGKSIARAARLALIAEEVNFLQV
Sbjct: 361 HSINGVGEEFQSEIINALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNFLQV 420

Query: 421 IPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHIG 480
           IPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGG +TQQGS+DSGADFGFGVYNDHH+HIG
Sbjct: 421 IPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGLITQQGSNDSGADFGFGVYNDHHYHIG 480

Query: 481 YFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASGL 540
           YFLYAIAVLVKIDPAWGRKF+  AYSLMADFMNLSRR NS FPRLRCFDLYKLHSWASGL
Sbjct: 481 YFLYAIAVLVKIDPAWGRKFKTHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASGL 540

Query: 541 TEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDN 600
           TEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTA+EIKAAQTWWQI+EGDN
Sbjct: 541 TEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEGDN 600

Query: 601 LYEVDFERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFARE 660
           LY+VDF RENKVVGVLWSNKRDSGLWFAP DWRECRLGIQVLPLLPITE+LFSD GF +E
Sbjct: 601 LYDVDFARENKVVGVLWSNKRDSGLWFAPADWRECRLGIQVLPLLPITEILFSDPGFVKE 660

Query: 661 LVEWTLPSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHSR 720
           LV+WTLPSLGREGVGEGWKGFAYALQGVYDKDGA+ +I+NL GFDDGNSLSNLLWWIHSR
Sbjct: 661 LVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGAVERIQNLTGFDDGNSLSNLLWWIHSR 720

Query: 721 GGG-----EGSGGGWRHWWLSH 723
           GGG     EG GGGW+HWW SH
Sbjct: 721 GGGGGDEGEGHGGGWKHWWFSH 739

BLAST of MC04g1785 vs. NCBI nr
Match: XP_008459427.1 (PREDICTED: probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucumis melo] >ADN34284.1 beta-glucan-binding protein [Cucumis melo subsp. melo] >KAA0039392.1 putative endo-1,3(4)-beta-glucanase [Cucumis melo var. makuwa] >TYK00580.1 putative endo-1,3(4)-beta-glucanase [Cucumis melo var. makuwa])

HSP 1 Score: 1244 bits (3218), Expect = 0.0
Identity = 621/742 (83.69%), Postives = 662/742 (89.22%), Query Frame = 0

Query: 1   MLKKLRRNFKRFKTLINKSF---------SSYKFPFQS----PPLSPPPPPAMSSPFEPL 60
           MLKKL+ NFK+FKTLINK+F         SS ++ F S    PPLSPPPP  MSSP +P 
Sbjct: 6   MLKKLKGNFKKFKTLINKTFNYSSSSSSSSSKRYKFSSSPSPPPLSPPPP-EMSSPSQPF 65

Query: 61  RQSPRPAAPVPFPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 120
            QSP P A + FPQTQSTVLPDPS+FF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL
Sbjct: 66  WQSPPPVAAL-FPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 125

Query: 121 IKSALSSIAISYPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDI 180
           IKS+LS+++ISYPS+  NSA GFQVFNAD TVS   N  S SQK HIIS FSDLS+TLDI
Sbjct: 126 IKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSE--NPSSVSQKPHIISSFSDLSLTLDI 185

Query: 181 PSANLRSFLVRGSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYS 240
           PS NLR FLVRGSPF+TFEVFNNT ISISTIHAILSFSSNSS TKFTVTLNNNQTWLIY+
Sbjct: 186 PSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYA 245

Query: 241 SSPINFTHDLSKITSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYK 300
           SSPIN TH LS+ITSGGF GI+RIA +P+PHCE+ILDRFSSCYPVSGE  F NPF LEYK
Sbjct: 246 SSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYK 305

Query: 301 WEKRGWGDLLMLAHPLHLRLLSGGGA---VLDDLKYKSIDGDLVGIVGSSWVLQSDPVSV 360
           WEK+GWG+LLMLAHPLHLRLL+G G+   VLD+ KYKSIDGDLVG+VG SWVL+ DPV V
Sbjct: 306 WEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVLKPDPVYV 365

Query: 361 TWHSINGVGAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFL 420
           TWHSINGVG EFHSEIISALVKDVEGL S+PITTT+SYFYGKSIARAARLALIAEEVN+L
Sbjct: 366 TWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALIAEEVNYL 425

Query: 421 QVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFH 480
           QVIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG VTQQGS DSG DFGFGVYNDHH+H
Sbjct: 426 QVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYH 485

Query: 481 IGYFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWAS 540
           IGYFLYAIAVLVKIDPAWGRKF+P AYSLMADFMNLSRR NS FPRLRCFDLYKLHSWAS
Sbjct: 486 IGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWAS 545

Query: 541 GLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREG 600
           GLTEFADGRNQESTSEAVN YYSAALLGLAYGDTHLASIGSTLTA+EIKAAQTWWQI+EG
Sbjct: 546 GLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEG 605

Query: 601 DNLYEVDFERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFA 660
           DNLYE DF RENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITE+LFSD  F 
Sbjct: 606 DNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDADFV 665

Query: 661 RELVEWTLPSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIH 720
           +ELV+WTLPSLGREGVGEGWKGFAYALQG+YDKDGAL KIRNL GFDDGNSLSNLLWWIH
Sbjct: 666 KELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLSNLLWWIH 725

Query: 721 SRGGG---EGSGGGWRHWWLSH 723
           SRGGG   EG GGGW+HWW SH
Sbjct: 726 SRGGGDEVEGCGGGWKHWWFSH 743

BLAST of MC04g1785 vs. NCBI nr
Match: XP_004141490.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucumis sativus] >KAE8648959.1 hypothetical protein Csa_007756 [Cucumis sativus])

HSP 1 Score: 1233 bits (3191), Expect = 0.0
Identity = 615/741 (83.00%), Postives = 660/741 (89.07%), Query Frame = 0

Query: 1   MLKKLRRNFKRFKTLINKSF--------SSYKFPFQS----PPLSPPPPPAMSSPFEPLR 60
           MLKKL+ NFK+ +TLINKSF        SS ++ F S    PPLSPPP   MSSP +P  
Sbjct: 6   MLKKLKGNFKKIRTLINKSFNNSSSSSSSSKRYKFSSSPSPPPLSPPPL-EMSSPSQPFW 65

Query: 61  QSPRPAAPVPFPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI 120
           QSP P A + FPQT+S+VLPDPS+FF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI
Sbjct: 66  QSPPPVAAL-FPQTESSVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI 125

Query: 121 KSALSSIAISYPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIP 180
           KS+LS++++SYPS++ NSA G+QVFNAD TVS   N  S SQK HIIS FSDLS+TLDIP
Sbjct: 126 KSSLSTVSVSYPSMLSNSAFGYQVFNADWTVSE--NPPSVSQKPHIISSFSDLSLTLDIP 185

Query: 181 SANLRSFLVRGSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSS 240
           S NLR FLVRGSPF+TFEVFNNT ISISTIHAILSFSSNSS TKFTVTLNNNQTWLIY+S
Sbjct: 186 SGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYAS 245

Query: 241 SPINFTHDLSKITSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKW 300
           SPIN TH LS+ITSGGF+GI+RIA +P+PHCE+ILDRFSSCYPVSGE  F NPF LEYKW
Sbjct: 246 SPINLTHSLSQITSGGFTGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYKW 305

Query: 301 EKRGWGDLLMLAHPLHLRLLSGGGA---VLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVT 360
           EK+GWG+LLMLAHPLHLRLLSG G+   VLDD KYKSIDGDLVG+VG SW L+ DPV +T
Sbjct: 306 EKKGWGNLLMLAHPLHLRLLSGDGSSAVVLDDFKYKSIDGDLVGVVGGSWALKPDPVYLT 365

Query: 361 WHSINGVGAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQ 420
           WHSINGVG EFHSEIISALVKDVEGL S+PITTTSSYFYGKSIARAARLALIAEEVN+LQ
Sbjct: 366 WHSINGVGEEFHSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNYLQ 425

Query: 421 VIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHI 480
           VIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG VTQQGS DSG DFGFGVYNDHH+HI
Sbjct: 426 VIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYHI 485

Query: 481 GYFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASG 540
           GYFLYAIAVLVKIDPAWGRKF+P AYSLMADFMNLSRR NS FPRLRCFDLYKLHSWASG
Sbjct: 486 GYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASG 545

Query: 541 LTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGD 600
           LTEFADGRNQESTSEAVN YYSAALLGLAYGDTHLASIGSTLTA+EIKAAQTWWQI+EGD
Sbjct: 546 LTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEGD 605

Query: 601 NLYEVDFERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFAR 660
           NLYE DF RENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+LFSD  F +
Sbjct: 606 NLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPILPITEILFSDADFVK 665

Query: 661 ELVEWTLPSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHS 720
           ELV+WTLPSLGREGVGEGWKGFAYALQGVYDKDGAL KIRNL GFDDGNSLSNLLWWIHS
Sbjct: 666 ELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGALEKIRNLTGFDDGNSLSNLLWWIHS 725

Query: 721 RGGG---EGSGGGWRHWWLSH 723
           RGGG   E  GGGW+HWW SH
Sbjct: 726 RGGGDEVEACGGGWKHWWFSH 742

BLAST of MC04g1785 vs. NCBI nr
Match: KAG7033239.1 (hypothetical protein SDJN02_07293, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1217 bits (3150), Expect = 0.0
Identity = 611/739 (82.68%), Postives = 651/739 (88.09%), Query Frame = 0

Query: 1   MLKKLRRNFKRFKTLINKSFSS-----------YKFPFQSPPLSPPPPPAMSSPFEPLRQ 60
           MLKKLRRNFK FKTLI+K+F++           YKFP    PLSP     MSSP +  RQ
Sbjct: 1   MLKKLRRNFKTFKTLISKTFNNSRSSSSSPSKPYKFPPLPLPLSPS---EMSSPSQSFRQ 60

Query: 61  SPRPAAPVPFPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK 120
           S  P A  PFPQTQSTVLPDPS+FF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK
Sbjct: 61  S-LPPAVAPFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK 120

Query: 121 SALSSIAISYPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPS 180
           S+LS+++ISYPS+  N+A G+QVFNAD TVS   N  S SQK HIIS FSDLS+ LD PS
Sbjct: 121 SSLSAVSISYPSMFSNTAFGYQVFNADWTVSE--NPASVSQKPHIISSFSDLSLILDTPS 180

Query: 181 ANLRSFLVRGSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSS 240
            NLR FLVRGSPF+TFEVFNNTPISISTIHAILSFSSNSS TKFTVTLNNNQTWLIYSSS
Sbjct: 181 GNLRFFLVRGSPFITFEVFNNTPISISTIHAILSFSSNSSQTKFTVTLNNNQTWLIYSSS 240

Query: 241 PINFTHDLSKITSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWE 300
           PIN TH LS+ITSGGFSGI+RIA +P+PH ESILDRF SCYPVSGE  F NP  LEYKWE
Sbjct: 241 PINLTHSLSQITSGGFSGIVRIAVLPNPHLESILDRFCSCYPVSGEVNFRNPSSLEYKWE 300

Query: 301 KRGWGDLLMLAHPLHLRLLSGGGA--VLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTWH 360
           K GWG+LLMLAHPLHLRLLS   +  VLDD KYKSIDGDLVG+VG SWVL+ DPVS+TWH
Sbjct: 301 KNGWGNLLMLAHPLHLRLLSSDDSAIVLDDFKYKSIDGDLVGVVGGSWVLKPDPVSITWH 360

Query: 361 SINGVGAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQVI 420
           SING+G EF SEIISALVKDVEGL S+PITTTSSYFYGK+IARAARLALIAEEVNFLQVI
Sbjct: 361 SINGIGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKAIARAARLALIAEEVNFLQVI 420

Query: 421 PEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHIGY 480
           PEVRKFLK AIEPWLRGTFNGNGFLYD KWGG VTQQG+SDSGADFGFGVYNDHH+HIGY
Sbjct: 421 PEVRKFLKDAIEPWLRGTFNGNGFLYDSKWGGLVTQQGASDSGADFGFGVYNDHHYHIGY 480

Query: 481 FLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASGLT 540
           FLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRR +S FPRLRCFDLYKLHSWASGLT
Sbjct: 481 FLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRSSSKFPRLRCFDLYKLHSWASGLT 540

Query: 541 EFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDNL 600
           EFADGRNQES+SEAVNAYYSAALLGLAYGDTHLASIGSTLTA+EIKAAQTWWQIR G+NL
Sbjct: 541 EFADGRNQESSSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIRGGENL 600

Query: 601 YEVDFERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFAREL 660
           YE DF RENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQ+LPLLPITE LFSD  FA+EL
Sbjct: 601 YETDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQLLPLLPITEALFSDAAFAKEL 660

Query: 661 VEWTLPSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHSRG 720
           V+WTLPSL REGVGEGWKGFAYALQG+YDKDGA+  IRNL GFDDGNSLSNLLWWIHSRG
Sbjct: 661 VDWTLPSLRREGVGEGWKGFAYALQGIYDKDGAVQNIRNLKGFDDGNSLSNLLWWIHSRG 720

Query: 721 GGE---GSGGGWRHWWLSH 723
           GGE   G GGGW+HWW SH
Sbjct: 721 GGEEGEGFGGGWKHWWFSH 733

BLAST of MC04g1785 vs. ExPASy TrEMBL
Match: A0A6J1BYQ9 (Endo-1,3(4)-beta-glucanase OS=Momordica charantia OX=3673 GN=LOC111006684 PE=3 SV=1)

HSP 1 Score: 1467 bits (3797), Expect = 0.0
Identity = 723/723 (100.00%), Postives = 723/723 (100.00%), Query Frame = 0

Query: 1   MLKKLRRNFKRFKTLINKSFSSYKFPFQSPPLSPPPPPAMSSPFEPLRQSPRPAAPVPFP 60
           MLKKLRRNFKRFKTLINKSFSSYKFPFQSPPLSPPPPPAMSSPFEPLRQSPRPAAPVPFP
Sbjct: 1   MLKKLRRNFKRFKTLINKSFSSYKFPFQSPPLSPPPPPAMSSPFEPLRQSPRPAAPVPFP 60

Query: 61  QTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSALSSIAISYP 120
           QTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSALSSIAISYP
Sbjct: 61  QTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSALSSIAISYP 120

Query: 121 SIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPSANLRSFLVRGS 180
           SIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPSANLRSFLVRGS
Sbjct: 121 SIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPSANLRSFLVRGS 180

Query: 181 PFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSSPINFTHDLSKI 240
           PFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSSPINFTHDLSKI
Sbjct: 181 PFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSSPINFTHDLSKI 240

Query: 241 TSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWEKRGWGDLLMLA 300
           TSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWEKRGWGDLLMLA
Sbjct: 241 TSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWEKRGWGDLLMLA 300

Query: 301 HPLHLRLLSGGGAVLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTWHSINGVGAEFHSEI 360
           HPLHLRLLSGGGAVLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTWHSINGVGAEFHSEI
Sbjct: 301 HPLHLRLLSGGGAVLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTWHSINGVGAEFHSEI 360

Query: 361 ISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPEVRKFLKGAIEP 420
           ISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPEVRKFLKGAIEP
Sbjct: 361 ISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPEVRKFLKGAIEP 420

Query: 421 WLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHIGYFLYAIAVLVKIDP 480
           WLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHIGYFLYAIAVLVKIDP
Sbjct: 421 WLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHIGYFLYAIAVLVKIDP 480

Query: 481 AWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASGLTEFADGRNQESTSE 540
           AWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASGLTEFADGRNQESTSE
Sbjct: 481 AWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASGLTEFADGRNQESTSE 540

Query: 541 AVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDNLYEVDFERENKVVG 600
           AVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDNLYEVDFERENKVVG
Sbjct: 541 AVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDNLYEVDFERENKVVG 600

Query: 601 VLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFARELVEWTLPSLGREGV 660
           VLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFARELVEWTLPSLGREGV
Sbjct: 601 VLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFARELVEWTLPSLGREGV 660

Query: 661 GEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHSRGGGEGSGGGWRHWW 720
           GEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHSRGGGEGSGGGWRHWW
Sbjct: 661 GEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHSRGGGEGSGGGWRHWW 720

Query: 721 LSH 723
           LSH
Sbjct: 721 LSH 723

BLAST of MC04g1785 vs. ExPASy TrEMBL
Match: A0A5A7T7D7 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001960 PE=3 SV=1)

HSP 1 Score: 1244 bits (3218), Expect = 0.0
Identity = 621/742 (83.69%), Postives = 662/742 (89.22%), Query Frame = 0

Query: 1   MLKKLRRNFKRFKTLINKSF---------SSYKFPFQS----PPLSPPPPPAMSSPFEPL 60
           MLKKL+ NFK+FKTLINK+F         SS ++ F S    PPLSPPPP  MSSP +P 
Sbjct: 6   MLKKLKGNFKKFKTLINKTFNYSSSSSSSSSKRYKFSSSPSPPPLSPPPP-EMSSPSQPF 65

Query: 61  RQSPRPAAPVPFPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 120
            QSP P A + FPQTQSTVLPDPS+FF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL
Sbjct: 66  WQSPPPVAAL-FPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 125

Query: 121 IKSALSSIAISYPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDI 180
           IKS+LS+++ISYPS+  NSA GFQVFNAD TVS   N  S SQK HIIS FSDLS+TLDI
Sbjct: 126 IKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSE--NPSSVSQKPHIISSFSDLSLTLDI 185

Query: 181 PSANLRSFLVRGSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYS 240
           PS NLR FLVRGSPF+TFEVFNNT ISISTIHAILSFSSNSS TKFTVTLNNNQTWLIY+
Sbjct: 186 PSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYA 245

Query: 241 SSPINFTHDLSKITSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYK 300
           SSPIN TH LS+ITSGGF GI+RIA +P+PHCE+ILDRFSSCYPVSGE  F NPF LEYK
Sbjct: 246 SSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYK 305

Query: 301 WEKRGWGDLLMLAHPLHLRLLSGGGA---VLDDLKYKSIDGDLVGIVGSSWVLQSDPVSV 360
           WEK+GWG+LLMLAHPLHLRLL+G G+   VLD+ KYKSIDGDLVG+VG SWVL+ DPV V
Sbjct: 306 WEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVLKPDPVYV 365

Query: 361 TWHSINGVGAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFL 420
           TWHSINGVG EFHSEIISALVKDVEGL S+PITTT+SYFYGKSIARAARLALIAEEVN+L
Sbjct: 366 TWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALIAEEVNYL 425

Query: 421 QVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFH 480
           QVIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG VTQQGS DSG DFGFGVYNDHH+H
Sbjct: 426 QVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYH 485

Query: 481 IGYFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWAS 540
           IGYFLYAIAVLVKIDPAWGRKF+P AYSLMADFMNLSRR NS FPRLRCFDLYKLHSWAS
Sbjct: 486 IGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWAS 545

Query: 541 GLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREG 600
           GLTEFADGRNQESTSEAVN YYSAALLGLAYGDTHLASIGSTLTA+EIKAAQTWWQI+EG
Sbjct: 546 GLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEG 605

Query: 601 DNLYEVDFERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFA 660
           DNLYE DF RENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITE+LFSD  F 
Sbjct: 606 DNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDADFV 665

Query: 661 RELVEWTLPSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIH 720
           +ELV+WTLPSLGREGVGEGWKGFAYALQG+YDKDGAL KIRNL GFDDGNSLSNLLWWIH
Sbjct: 666 KELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLSNLLWWIH 725

Query: 721 SRGGG---EGSGGGWRHWWLSH 723
           SRGGG   EG GGGW+HWW SH
Sbjct: 726 SRGGGDEVEGCGGGWKHWWFSH 743

BLAST of MC04g1785 vs. ExPASy TrEMBL
Match: E5GCT5 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1244 bits (3218), Expect = 0.0
Identity = 621/742 (83.69%), Postives = 662/742 (89.22%), Query Frame = 0

Query: 1   MLKKLRRNFKRFKTLINKSF---------SSYKFPFQS----PPLSPPPPPAMSSPFEPL 60
           MLKKL+ NFK+FKTLINK+F         SS ++ F S    PPLSPPPP  MSSP +P 
Sbjct: 6   MLKKLKGNFKKFKTLINKTFNYSSSSSSSSSKRYKFSSSPSPPPLSPPPP-EMSSPSQPF 65

Query: 61  RQSPRPAAPVPFPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 120
            QSP P A + FPQTQSTVLPDPS+FF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL
Sbjct: 66  WQSPPPVAAL-FPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 125

Query: 121 IKSALSSIAISYPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDI 180
           IKS+LS+++ISYPS+  NSA GFQVFNAD TVS   N  S SQK HIIS FSDLS+TLDI
Sbjct: 126 IKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSE--NPSSVSQKPHIISSFSDLSLTLDI 185

Query: 181 PSANLRSFLVRGSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYS 240
           PS NLR FLVRGSPF+TFEVFNNT ISISTIHAILSFSSNSS TKFTVTLNNNQTWLIY+
Sbjct: 186 PSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYA 245

Query: 241 SSPINFTHDLSKITSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYK 300
           SSPIN TH LS+ITSGGF GI+RIA +P+PHCE+ILDRFSSCYPVSGE  F NPF LEYK
Sbjct: 246 SSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYK 305

Query: 301 WEKRGWGDLLMLAHPLHLRLLSGGGA---VLDDLKYKSIDGDLVGIVGSSWVLQSDPVSV 360
           WEK+GWG+LLMLAHPLHLRLL+G G+   VLD+ KYKSIDGDLVG+VG SWVL+ DPV V
Sbjct: 306 WEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVLKPDPVYV 365

Query: 361 TWHSINGVGAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFL 420
           TWHSINGVG EFHSEIISALVKDVEGL S+PITTT+SYFYGKSIARAARLALIAEEVN+L
Sbjct: 366 TWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALIAEEVNYL 425

Query: 421 QVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFH 480
           QVIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG VTQQGS DSG DFGFGVYNDHH+H
Sbjct: 426 QVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYH 485

Query: 481 IGYFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWAS 540
           IGYFLYAIAVLVKIDPAWGRKF+P AYSLMADFMNLSRR NS FPRLRCFDLYKLHSWAS
Sbjct: 486 IGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWAS 545

Query: 541 GLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREG 600
           GLTEFADGRNQESTSEAVN YYSAALLGLAYGDTHLASIGSTLTA+EIKAAQTWWQI+EG
Sbjct: 546 GLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEG 605

Query: 601 DNLYEVDFERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFA 660
           DNLYE DF RENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITE+LFSD  F 
Sbjct: 606 DNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDADFV 665

Query: 661 RELVEWTLPSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIH 720
           +ELV+WTLPSLGREGVGEGWKGFAYALQG+YDKDGAL KIRNL GFDDGNSLSNLLWWIH
Sbjct: 666 KELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLSNLLWWIH 725

Query: 721 SRGGG---EGSGGGWRHWWLSH 723
           SRGGG   EG GGGW+HWW SH
Sbjct: 726 SRGGGDEVEGCGGGWKHWWFSH 743

BLAST of MC04g1785 vs. ExPASy TrEMBL
Match: A0A1S3CAN8 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=LOC103498567 PE=3 SV=1)

HSP 1 Score: 1244 bits (3218), Expect = 0.0
Identity = 621/742 (83.69%), Postives = 662/742 (89.22%), Query Frame = 0

Query: 1   MLKKLRRNFKRFKTLINKSF---------SSYKFPFQS----PPLSPPPPPAMSSPFEPL 60
           MLKKL+ NFK+FKTLINK+F         SS ++ F S    PPLSPPPP  MSSP +P 
Sbjct: 6   MLKKLKGNFKKFKTLINKTFNYSSSSSSSSSKRYKFSSSPSPPPLSPPPP-EMSSPSQPF 65

Query: 61  RQSPRPAAPVPFPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 120
            QSP P A + FPQTQSTVLPDPS+FF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL
Sbjct: 66  WQSPPPVAAL-FPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 125

Query: 121 IKSALSSIAISYPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDI 180
           IKS+LS+++ISYPS+  NSA GFQVFNAD TVS   N  S SQK HIIS FSDLS+TLDI
Sbjct: 126 IKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSE--NPSSVSQKPHIISSFSDLSLTLDI 185

Query: 181 PSANLRSFLVRGSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYS 240
           PS NLR FLVRGSPF+TFEVFNNT ISISTIHAILSFSSNSS TKFTVTLNNNQTWLIY+
Sbjct: 186 PSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYA 245

Query: 241 SSPINFTHDLSKITSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYK 300
           SSPIN TH LS+ITSGGF GI+RIA +P+PHCE+ILDRFSSCYPVSGE  F NPF LEYK
Sbjct: 246 SSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYK 305

Query: 301 WEKRGWGDLLMLAHPLHLRLLSGGGA---VLDDLKYKSIDGDLVGIVGSSWVLQSDPVSV 360
           WEK+GWG+LLMLAHPLHLRLL+G G+   VLD+ KYKSIDGDLVG+VG SWVL+ DPV V
Sbjct: 306 WEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVLKPDPVYV 365

Query: 361 TWHSINGVGAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFL 420
           TWHSINGVG EFHSEIISALVKDVEGL S+PITTT+SYFYGKSIARAARLALIAEEVN+L
Sbjct: 366 TWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALIAEEVNYL 425

Query: 421 QVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFH 480
           QVIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG VTQQGS DSG DFGFGVYNDHH+H
Sbjct: 426 QVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYH 485

Query: 481 IGYFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWAS 540
           IGYFLYAIAVLVKIDPAWGRKF+P AYSLMADFMNLSRR NS FPRLRCFDLYKLHSWAS
Sbjct: 486 IGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWAS 545

Query: 541 GLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREG 600
           GLTEFADGRNQESTSEAVN YYSAALLGLAYGDTHLASIGSTLTA+EIKAAQTWWQI+EG
Sbjct: 546 GLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEG 605

Query: 601 DNLYEVDFERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFA 660
           DNLYE DF RENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITE+LFSD  F 
Sbjct: 606 DNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDADFV 665

Query: 661 RELVEWTLPSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIH 720
           +ELV+WTLPSLGREGVGEGWKGFAYALQG+YDKDGAL KIRNL GFDDGNSLSNLLWWIH
Sbjct: 666 KELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLSNLLWWIH 725

Query: 721 SRGGG---EGSGGGWRHWWLSH 723
           SRGGG   EG GGGW+HWW SH
Sbjct: 726 SRGGGDEVEGCGGGWKHWWFSH 743

BLAST of MC04g1785 vs. ExPASy TrEMBL
Match: A0A0A0KSJ9 (Endo-1,3(4)-beta-glucanase OS=Cucumis sativus OX=3659 GN=Csa_5G643880 PE=3 SV=1)

HSP 1 Score: 1233 bits (3191), Expect = 0.0
Identity = 615/741 (83.00%), Postives = 660/741 (89.07%), Query Frame = 0

Query: 1   MLKKLRRNFKRFKTLINKSF--------SSYKFPFQS----PPLSPPPPPAMSSPFEPLR 60
           MLKKL+ NFK+ +TLINKSF        SS ++ F S    PPLSPPP   MSSP +P  
Sbjct: 1   MLKKLKGNFKKIRTLINKSFNNSSSSSSSSKRYKFSSSPSPPPLSPPPL-EMSSPSQPFW 60

Query: 61  QSPRPAAPVPFPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI 120
           QSP P A + FPQT+S+VLPDPS+FF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI
Sbjct: 61  QSPPPVAAL-FPQTESSVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI 120

Query: 121 KSALSSIAISYPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIP 180
           KS+LS++++SYPS++ NSA G+QVFNAD TVS   N  S SQK HIIS FSDLS+TLDIP
Sbjct: 121 KSSLSTVSVSYPSMLSNSAFGYQVFNADWTVSE--NPPSVSQKPHIISSFSDLSLTLDIP 180

Query: 181 SANLRSFLVRGSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSS 240
           S NLR FLVRGSPF+TFEVFNNT ISISTIHAILSFSSNSS TKFTVTLNNNQTWLIY+S
Sbjct: 181 SGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYAS 240

Query: 241 SPINFTHDLSKITSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKW 300
           SPIN TH LS+ITSGGF+GI+RIA +P+PHCE+ILDRFSSCYPVSGE  F NPF LEYKW
Sbjct: 241 SPINLTHSLSQITSGGFTGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYKW 300

Query: 301 EKRGWGDLLMLAHPLHLRLLSGGGA---VLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVT 360
           EK+GWG+LLMLAHPLHLRLLSG G+   VLDD KYKSIDGDLVG+VG SW L+ DPV +T
Sbjct: 301 EKKGWGNLLMLAHPLHLRLLSGDGSSAVVLDDFKYKSIDGDLVGVVGGSWALKPDPVYLT 360

Query: 361 WHSINGVGAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQ 420
           WHSINGVG EFHSEIISALVKDVEGL S+PITTTSSYFYGKSIARAARLALIAEEVN+LQ
Sbjct: 361 WHSINGVGEEFHSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNYLQ 420

Query: 421 VIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHI 480
           VIPEVRKFLKGAIEPWL GTFNGNGFLYDGKWGG VTQQGS DSG DFGFGVYNDHH+HI
Sbjct: 421 VIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYHI 480

Query: 481 GYFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASG 540
           GYFLYAIAVLVKIDPAWGRKF+P AYSLMADFMNLSRR NS FPRLRCFDLYKLHSWASG
Sbjct: 481 GYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASG 540

Query: 541 LTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGD 600
           LTEFADGRNQESTSEAVN YYSAALLGLAYGDTHLASIGSTLTA+EIKAAQTWWQI+EGD
Sbjct: 541 LTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEGD 600

Query: 601 NLYEVDFERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFAR 660
           NLYE DF RENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+LFSD  F +
Sbjct: 601 NLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPILPITEILFSDADFVK 660

Query: 661 ELVEWTLPSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHS 720
           ELV+WTLPSLGREGVGEGWKGFAYALQGVYDKDGAL KIRNL GFDDGNSLSNLLWWIHS
Sbjct: 661 ELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGALEKIRNLTGFDDGNSLSNLLWWIHS 720

Query: 721 RGGG---EGSGGGWRHWWLSH 723
           RGGG   E  GGGW+HWW SH
Sbjct: 721 RGGGDEVEACGGGWKHWWFSH 737

BLAST of MC04g1785 vs. TAIR 10
Match: AT5G15870.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 939.5 bits (2427), Expect = 1.6e-273
Identity = 480/750 (64.00%), Postives = 572/750 (76.27%), Query Frame = 0

Query: 1   MLKKLRRNFKRFKTLINKSFSSYKFPFQSPPLSPPPPPAMSSPFEP----LRQSPRPAAP 60
           MLKK+RR   + K LI K F   K P   PP  PPP P   SP  P    +  S +   P
Sbjct: 1   MLKKVRR---KVKVLITKPF---KKPKNRPPSPPPPLPLPPSPSPPPSQQMSSSRQKNTP 60

Query: 61  VPFPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSALSSIA 120
             FP++ S+VLPDPS FFS  LLSSPLPTNSFFQNFT+ NGDQ EY HPY+IK + SS++
Sbjct: 61  FLFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEYFHPYIIKPSTSSLS 120

Query: 121 ISYPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPSANLRSFL 180
           ISYPS+  NSA  ++ FNAD+T++ +      S+KSH+IS FSDL VTLD PS+NLR FL
Sbjct: 121 ISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLGVTLDFPSSNLRFFL 180

Query: 181 VRGSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSSPINFTHD 240
           VRGSPF+TF V  N+ I+ISTIHA+LS S N+S TK+TV LNNNQTWLIY+SSPIN T D
Sbjct: 181 VRGSPFITFSV--NSSITISTIHAVLSLSGNTSSTKYTVKLNNNQTWLIYASSPINLTKD 240

Query: 241 -LSKITSG-GFSGIIRIAAV--PDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWEKRG 300
            +S I  G GFSGIIRI  +  P+P+ E+ILD FS  YPVSG+A F  PF LEYKWEKRG
Sbjct: 241 GVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADFTKPFALEYKWEKRG 300

Query: 301 WGDLLMLAHPLHLRLLSGGG---AVLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTWHSI 360
           +GDLLMLAHPLHL+LLS       VLD+ KY SIDGDLVG++G SWVL+ DPVSVTWHSI
Sbjct: 301 YGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSWVLKPDPVSVTWHSI 360

Query: 361 NGVGAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPE 420
            GV  + H EIISAL+KDV  L+S+   T SSYFY K IARAARLALIAEEV +L VIP+
Sbjct: 361 KGVQEDSHQEIISALIKDVNALDSSAEVTNSSYFYAKLIARAARLALIAEEVCYLDVIPK 420

Query: 421 VRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHIGYFL 480
           +R +LK  IEPWL G+F  NGFLYD KWGG +T+ GS DSGADFGFG+YNDHH+H+GYF+
Sbjct: 421 IRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGFGIYNDHHYHLGYFV 480

Query: 481 YAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSR---RPNSSFPRLRCFDLYKLHSWASGL 540
           YAIAVL KIDP WG+++RPQAY+LMAD++ L +   + NS++PRLRCFDL+KLHSWA GL
Sbjct: 481 YAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLRCFDLFKLHSWAGGL 540

Query: 541 TEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDN 600
           TEFADGRNQESTSEAVNAYYSAALLGLAYGDTHL +  S +  +EI AA+ WWQ++E D 
Sbjct: 541 TEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEIHAAKMWWQVKEDDA 600

Query: 601 LYEVDFERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFARE 660
           +Y  DF  EN+VVGVLWS KRDSGLWFAP +W+ECRLGIQ+LPLLP++EVLFSDV F ++
Sbjct: 601 IYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLPVSEVLFSDVTFVKQ 660

Query: 661 LVEWTLPSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHSR 720
           LV WT+P+L R+ VGEGWKGF YAL+ +YDKDGA+ KI+ L GFDDGNSLSNLLWW+HSR
Sbjct: 661 LVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDDGNSLSNLLWWVHSR 720

Query: 721 -------------GGGEGSGGGWRHWWLSH 724
                        GG  G GGG ++    H
Sbjct: 721 NNDDDDDYEEDDEGGYGGHGGGGKYCSFGH 742

BLAST of MC04g1785 vs. TAIR 10
Match: AT1G18310.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 821.6 bits (2121), Expect = 4.9e-238
Identity = 419/679 (61.71%), Postives = 489/679 (72.02%), Query Frame = 0

Query: 59  FPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSALSSIAIS 118
           FP+T+S+VLPDPS FFSP LLS+PLPTNSFFQNFT+KNGDQ EY HPYLIKS  SS+ IS
Sbjct: 10  FPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVDSSLCIS 69

Query: 119 YPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPSANLRSFLVR 178
           YPS+  NS   ++VF AD+ +S +   G  S+K+HIIS FSDL VTLD PS+NLR FLVR
Sbjct: 70  YPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNLRFFLVR 129

Query: 179 GSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSSPINFTH--D 238
           GSP                          SS TK++  L NNQ WLIY+SSPI+ T   D
Sbjct: 130 GSP--------------------------SSSTKWSDKLTNNQRWLIYASSPIDLTKHGD 189

Query: 239 LSKITSGGFSGIIRIAAVP--DPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWEKRGWG 298
            S    GGF+GI+RI  +P  +P  ES LDRFSSCYPVSG+A F  PF L+Y WEKRG G
Sbjct: 190 SSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMWEKRGSG 249

Query: 299 DLLMLAHPLHLRLLSGGGA-----------------------VLDDLKYKSIDGDLVGIV 358
           DLLMLAHPLHL+LL+   +                       VLD  +YKSIDGDLVG+V
Sbjct: 250 DLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDGDLVGVV 309

Query: 359 GSSWVLQSDPVSVTWHSINGVGAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARA 418
           G SWVL+ D VSVTWHS+ GV  + + EIISAL KDV GLNS+   T+SSYFYGK IARA
Sbjct: 310 GDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLNSSAEVTSSSYFYGKLIARA 369

Query: 419 ARLALIAEEVNFLQVIPEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGA 478
           AR ALIAEEV +L VIP++  +LK  IEPWL G+F  NGFLYD KWGG +T+QGS DS A
Sbjct: 370 ARFALIAEEVCYLDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITKQGSKDSQA 429

Query: 479 DFGFGVYNDHHFHIGYFLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRR----PNSS 538
           DFGFG+YNDHH+HIGYFLYAIAVL K DP WG ++R QAYSL+ADFM   R+     NSS
Sbjct: 430 DFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRKDDNNSNSS 489

Query: 539 FPRLRCFDLYKLHSWASGLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTL 598
           +PRLR FDL+KLHSWA GLTEF DGRNQESTSEAVNAYYSAALLGLAYGD HL    ST+
Sbjct: 490 YPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKHLVETASTI 549

Query: 599 TAMEIKAAQTWWQIREGDNLYEVDFERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQV 658
             +EI AA+ WWQ+++G+ LY  DF  EN+VVGVLWS KRDS LWF P +W+ECRLGIQ+
Sbjct: 550 MTLEIHAAKMWWQVKKGEALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWKECRLGIQL 609

Query: 659 LPLLPITEVLFSDVGFARELVEWTLPSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNL 707
           LP+LP              LV WTLP+L R GVGEGWKGF YAL+ +YDKDGA+ KI+ L
Sbjct: 610 LPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDGAIKKIKRL 648