MC04g1173 (gene) Bitter gourd (Dali-11) v1

BLAST of MC04g1173 vs. ExPASy Swiss-Prot
Match: I3PB36 (Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 5.5e-213
Identity = 358/558 (64.16%), Postives = 429/558 (76.88%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M+ L KC +N+  LTP++FL RA  SYA+RTS+IY   RFTW  T++RC RLASSL+SLN
Sbjct: 1   MDELPKCGANYVPLTPLTFLTRAFKSYANRTSIIYAGARFTWEQTYKRCCRLASSLQSLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           + KNDVVSV+APNVPA YEMHFAVPMAGAVLNTINTRLD  NIA+IL+HSEAK+LFVDY+
Sbjct: 61  IVKNDVVSVLAPNVPATYEMHFAVPMAGAVLNTINTRLDPMNIAIILKHSEAKLLFVDYE 120

Query: 121 YIPQAKDALRLLV---------AESSPLPLVVVIDDIDSPTGI-RFGELEYEDLIRNGNP 180
           Y+ +A+ AL LL+         ++   +P V++IDD+ SPT I +  +LEYE L+  GNP
Sbjct: 121 YLEKARKALELLMSTNFITAQNSKKISMPQVILIDDLYSPTRIQQQDQLEYEQLVHQGNP 180

Query: 181 EFVAVE-VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTL 240
           E+     VDDEWD I LNYTSGTTS PKGVVYSHRGAFLSTL+ +MGWEMG  PVYLW+L
Sbjct: 181 EYAPENIVDDEWDPIVLNYTSGTTSEPKGVVYSHRGAFLSTLNTIMGWEMGTEPVYLWSL 240

Query: 241 PMFHCNGWTFTWGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESA 300
           PMFH NGWT TWGIAARGGTNVC+RNTTA +IY NI +H VTHMCCAP VF I+LEA+  
Sbjct: 241 PMFHINGWTLTWGIAARGGTNVCIRNTTAQEIYSNITLHKVTHMCCAPTVFNILLEAKPH 300

Query: 301 DRRRVGSPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPS 360
           +RR + +PV V+ GGAPPP  L+ K+E LGFH+ H YG+TEA G  LVCEWQ +WN L  
Sbjct: 301 ERREITTPVQVMVGGAPPPTTLIGKIEELGFHVVHCYGITEAGGTTLVCEWQSEWNKLSR 360

Query: 361 DQQAKLKARQGIGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATA 420
           + QA LKARQGI +L L DVDVKN +TM+SVPH+G+T GEI LRGSSIMKGY K+  A +
Sbjct: 361 EDQANLKARQGISVLALEDVDVKNSKTMQSVPHNGKTMGEICLRGSSIMKGYFKNDKANS 420

Query: 421 AAFKNGWFLTGDVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVV 480
             FKNGWFLTGDV VIH DGYLEI+DR KD+IISGGENISS+E+E  +  HP V EAAVV
Sbjct: 421 QVFKNGWFLTGDVAVIHQDGYLEIKDRCKDIIISGGENISSIEVENAILKHPSVIEAAVV 480

Query: 481 AMPHPRWGESPCAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTA 540
           AMPHPRWGE+PCAF+            + EA+I+  C+  LP +MVPK V F+ ELPKT 
Sbjct: 481 AMPHPRWGETPCAFVIKTKNPE-----IKEADIIVHCKKELPGFMVPKHVQFLEELPKTG 540

Query: 541 TGKIQKFQLREQAKLFNV 548
           TGK++K QLRE AK F +
Sbjct: 541 TGKVKKLQLREMAKSFGI 553

BLAST of MC04g1173 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 4.4e-202
Identity = 339/554 (61.19%), Postives = 426/554 (76.90%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M++L  C++N   LTPI+FL+RAS  Y +RTS+IY +TRFTW  T+ RC RLA+SL SLN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           +++NDVVS++APNVPA+YEMHF+VPM GAVLN INTRLD K IA+ILRH+E K+LFVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIPQAKDALRLL-VAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNP----EFVAV 180
           + P  ++ LRL+   +S P P +++I++IDS T     EL+YE LIR G P         
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            V +E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVG 300
           GWT TW +AARGGTNVC+R+ TA +IY+NI +H VTHM C P VFR +LE    D+    
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 SPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKL 360
           SPV VLTGG+ PPA L++K+E LGFH+ H YGLTEATGP L CEWQ +WN LP  QQ +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGIGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNG 420
           + RQG+  LTLADVDVKN +T+ESVP DG+T GEIV++GSS+MKGYLK+P AT+ AFK+G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPR 480
           W  TGD+GVIHPDGY+EI+DRSKD+IISGGENISS+E+E VLY +  V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQK 540
           WGE+PCAF+ +  G  G     +E +++++CR N+PH+M PKKVVF  ELPK + GKI K
Sbjct: 481 WGETPCAFVVLKKGEEGLV--TSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILK 540

Query: 541 FQLREQAKLFNVSE 550
            +LR+ AK   V E
Sbjct: 541 SKLRDIAKALVVRE 552

BLAST of MC04g1173 vs. ExPASy Swiss-Prot
Match: Q9SS00 (Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE12 PE=2 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 1.8e-200
Identity = 339/553 (61.30%), Postives = 423/553 (76.49%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M++L+ C++N   LTPI+FL+RAS  Y +RTS+IY +TRFTW  T+ RC RLA+SL SLN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           + KNDVVSV+APN PA+YEMHFAVPMAGAVLN INTRLD  +IA ILRH++ K+LF+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIPQAKDALRLLVAESSPLPL-VVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVE--- 180
           + P A++ L+LL +E S L L V+ I +ID P  +   E +YE LI+ G P  + +    
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARMF 180

Query: 181 -VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 CIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVG 300
           GWTFTWG AARGGT+VCMR+ TA +IY+NI MH+VTHMCC P VF I+L+  S D     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRS 300

Query: 301 SPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKL 360
            PV+VLTGG+PPPA L++K++ LGF + HAYGLTEATGP L CEWQ +WN LP +QQ +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGIGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNG 420
           KARQG+ IL L +VDV+N ET ESVP DG+T GEIV++GSSIMKGYLK+P AT  AFK+G
Sbjct: 361 KARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPR 480
           W  +GDVGVIHPDG++EI+DRSKD+IISGGENISSVE+E ++Y +P V E AVVAMPHP 
Sbjct: 421 WLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFIAVHGGAHGGAGG-----VTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTAT 540
           WGE+PCAF+ +  G              E +++E+CR NLPH+M P+KVVF+ ELPK   
Sbjct: 481 WGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKNGN 540

Query: 541 GKIQKFQLREQAK 544
           GKI K +LR+ AK
Sbjct: 541 GKILKPKLRDIAK 553

BLAST of MC04g1173 vs. ExPASy Swiss-Prot
Match: Q9SS01 (Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 6.0e-199
Identity = 344/554 (62.09%), Postives = 419/554 (75.63%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M+ L+ C++N   LTP++FL+RAS  Y +RTS+IY +TRFTW  T+ RC RLA+SL SLN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           +SKNDVVSV+APN PALYEMHFAVPMAGAVLN INTRLD  +IA ILRH++ K+LF+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIPQAKDALRLLVAESSPLPL-VVVIDDIDSPTGIRFGELEYEDLIRNG--NPEFVA--V 180
           +   A+++L LL +E S L L V+ I + D P    F EL+YE LI+ G   P  VA   
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVG 300
           GWTFTWG AARGGT+VCMR+ TA +IY+NI MH+VTHMCC P VF I+L+  S D     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 SPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKL 360
            PV+VLTGG+PPPA L++K++ LGF + HAYG TEATGP L CEWQ +WN LP +QQ +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGIGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNG 420
           KARQGI IL LADVDVKN ET +S P DG+T GEI+++GSSIMKGYLK+P AT  AFK+G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPR 480
           W  TGDVGVIHPDG++EI+DRSKD+IISGGENISSVE+E VLY +P V E AVVAMPHP 
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFIAVHGGA------HGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTA 540
           WGE+PCAF+ +                  E  ++E+CR NLPH+M P+KVVF+ ELPK  
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540

Query: 541 TGKIQKFQLREQAK 544
            GKI K +LR+ AK
Sbjct: 541 NGKILKPKLRDIAK 554

BLAST of MC04g1173 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 3.0e-166
Identity = 284/555 (51.17%), Postives = 395/555 (71.17%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           ME   K  +N+  LTPISFL R++  YADR S++Y   ++TWR T  RC R+AS+L  L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           +S  DVVSV+APNVPA+ E+HF VPMAGA+L T+N R D   +AV+LRHS  KV+F D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YIPQAKDALRLLVAESSPLPLVVVIDD--IDSPTGIRFGE--LEYEDLIRNGNPEFVAVE 180
           ++  A+ A  +L  +   +P++V+I +    S +  +  E  +EYED++  G  +F  + 
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGS 300
           W   WG+ A GGTN+C+RN TA  I+ NI+ H VTHM  AP +  +I+ A  ++++ +  
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 PVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLK 360
            V+ +TG APPPA ++ KME LGF + H+YGLTE  GP  +C W+ +W+SLP ++QAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGIGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGW 420
           ARQG+  L L ++ VK+  TM ++P DG T GE+V RG+++M GYLK+P AT  AFK GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRW 480
           F +GD+GV HPDGY+E++DRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKF 540
           GE+ CAF+ +  G+   A      E++ +CR  LPHYM P+ +VF  +LPKT+TGK+QKF
Sbjct: 483 GETACAFVKLKDGSKASA-----EELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKF 542

Query: 541 QLREQAK-LFNVSEK 551
            LR +AK L ++S+K
Sbjct: 543 VLRTKAKALVSLSKK 551

BLAST of MC04g1173 vs. NCBI nr
Match: XP_022923630.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 998 bits (2581), Expect = 0.0
Identity = 487/579 (84.11%), Postives = 524/579 (90.50%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M SLSKCD+N+ +LTPI+FL+RASA YADRTS+IYE TRFTWR T+QRC RLASSL  LN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           V+KN VVSV+APNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+LFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIPQAKDALRLLVAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVEVDDE 180
           +I +AKDALR L+A S PLP VVVIDDIDSPTG RFGELEYE L+ +G+  F  VEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGSPVNV 300
           WGIAARGGTNVCMRNTTA+DIYRNIN+HHVTHMCCAPIVFRIILEA   +RRR G  VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQ KWN LP+D+QAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 IGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGWFLTG 420
           I ILTLADVDVKNLETM+SVPHDGRT GEIVLRGSSIMKGY KDPAATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEI+DRSKDVIISGGENISSVE+ETVLY HP VAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKFQLRE 540
           CAF+ VH       G V+EAE++EFCR N+ HYM PKKVVFVAELPKT TGKIQKF+LRE
Sbjct: 481 CAFVVVHSDG----GKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFNVSEKRVSGGNKSGRQDGGRRADYGEHVLAMSRL 579
           QAKL   SEKRVSGGNKSGR DGGR+ DYG+HV+AMSRL
Sbjct: 541 QAKLLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575

BLAST of MC04g1173 vs. NCBI nr
Match: KAG6602004.1 (Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 994 bits (2571), Expect = 0.0
Identity = 486/579 (83.94%), Postives = 523/579 (90.33%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M SLSKCD+N+ +LTPI+FL+RASA YADRTS+IYE TRFTWR T+QRC RLASSL  LN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           V+KN VVSV+APNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+LFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIPQAKDALRLLVAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVEVDDE 180
           +I +AKDALR L+A S PLP VVVIDDIDSPTG RFGELEYE L+ +G+  F  VEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGSPVNV 300
           WGIAARGGTNVCMRNTTA+DIYRNIN+HHVTHMCCAPIVFRIILEA   +RRR G  VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQ KWN LP+D+QAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 IGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGWFLTG 420
           I ILTLADVDVKNLETM+SVPHDGRT GEIVLRGSSIMKGY KD AATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDLAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEI+DRSKDVIISGGENISSVE+ETVLY HP VAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKFQLRE 540
           CAF+ VH       G V+EAE++EFCR N+ HYM PKKVVFVAELPKT TGKIQKF+LRE
Sbjct: 481 CAFVVVHSDG----GKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFNVSEKRVSGGNKSGRQDGGRRADYGEHVLAMSRL 579
           QAKL   SEKRVSGGNKSGR DGGR+ DYG+HV+AMSRL
Sbjct: 541 QAKLLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575

BLAST of MC04g1173 vs. NCBI nr
Match: XP_023513711.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 993 bits (2567), Expect = 0.0
Identity = 484/579 (83.59%), Postives = 523/579 (90.33%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M SLSKCD+N+ +LTPI+FL+RASA YADRTS+IYE TRFTWR T++RC RLASSL  LN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLSRLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           V KN VVSV+APNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+LFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIPQAKDALRLLVAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVEVDDE 180
           +I +AKDALR L+A S PLP VVVIDDIDSPTG RFGELEYE L+ +G+  F  VEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLV+GWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVVGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGSPVNV 300
           WGIAARGGTNVCMRNTTA+DIYRNIN+HHVTHMCCAPIVFRIILEA   +RRR G  VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQ KWN LP+D+QAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 IGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGWFLTG 420
           I ILTLADVDVKNLETM+SVPHDGRT GEIVLRGSSIMKGY KDPAATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEI+DRSKDVIISGGENISSVE+ETVLY HP VAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKFQLRE 540
           CAF+ VH       G V+EAE++EFCR N+ HYM PKKVVFVAELPKT TGKIQKF+LRE
Sbjct: 481 CAFVVVHSDG----GKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFNVSEKRVSGGNKSGRQDGGRRADYGEHVLAMSRL 579
           QAKL   SEKRVSGGNKSGR DGGR+ +YG+HV+AMSRL
Sbjct: 541 QAKLLVGSEKRVSGGNKSGRHDGGRQPEYGQHVMAMSRL 575

BLAST of MC04g1173 vs. NCBI nr
Match: XP_038906606.1 (trans-cinnamate:CoA ligase, peroxisomal-like [Benincasa hispida])

HSP 1 Score: 993 bits (2567), Expect = 0.0
Identity = 485/581 (83.48%), Postives = 525/581 (90.36%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M+ L KCD+N+ +LTPI+FL RAS  YADRTS+IYE TRFTW  T++RC RLASSLR LN
Sbjct: 1   MDMLLKCDANYTSLTPITFLTRASTFYADRTSIIYEGTRFTWHQTYERCCRLASSLRHLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           +SKNDVVSV+APNVPALYEMHFAVPMAGA+LNTINTRLDV NIAVILRHSEAK+LFVDYQ
Sbjct: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIAVILRHSEAKILFVDYQ 120

Query: 121 YIPQAKDALRLLVAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVEVDDE 180
           YI +AKDALRLL+A+S P+PLVV+IDDIDSPTGIRFG  EYE LI +G+  F  +EVDDE
Sbjct: 121 YIQEAKDALRLLMAKSFPMPLVVIIDDIDSPTGIRFGNFEYEKLIHDGDVNFTPLEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGSPVNV 300
           WGIAARGGTN+CMRNTTA DI+RNIN+HHVTHMCCAPIVFRIIL+A S DR RV  P+NV
Sbjct: 241 WGIAARGGTNICMRNTTAIDIFRNINLHHVTHMCCAPIVFRIILDANSHDRCRVAWPINV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLKARQG 360
           LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQ KWN+LP+D+QAKLKARQG
Sbjct: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNTLPADKQAKLKARQG 360

Query: 361 IGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGWFLTG 420
           I ILTLADVDVKNL+TMESVPHDG T GEIVLRGS +MKGY KDP ATAAAFKNGWFLTG
Sbjct: 361 ISILTLADVDVKNLDTMESVPHDGHTTGEIVLRGSGLMKGYFKDPVATAAAFKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEI+DRSKDVIISGGENISSVE+ETVLY HP VAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYSHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKFQLRE 540
           CAF+AVHG A    G V+EAEI+ FCR NLPHYMVPKKVVFV ELPK A GKIQKF+LRE
Sbjct: 481 CAFVAVHGDA----GRVSEAEIIAFCRKNLPHYMVPKKVVFVGELPK-ANGKIQKFKLRE 540

Query: 541 QAKLFNVSEKRV--SGGNKSGRQDGGRRADYGEHVLAMSRL 579
           QAK   VSEKRV  +G NKS RQDG RR+DYGEHVLAMSRL
Sbjct: 541 QAKYLEVSEKRVVGTGWNKSSRQDGNRRSDYGEHVLAMSRL 576

BLAST of MC04g1173 vs. NCBI nr
Match: XP_022990938.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 990 bits (2560), Expect = 0.0
Identity = 483/579 (83.42%), Postives = 521/579 (89.98%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M SLSKCD+NF +LTPI+FL+RASA YADRTS+IYE T FTWR T++RC  LASSL  LN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           V KN VVSV+APNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+LFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIPQAKDALRLLVAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVEVDDE 180
           +I +AKDALR L+A S PLP VVVIDDIDSPTG RFGELEYE L+ +G+  F  VEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGSPVNV 300
           WGIAARGGTNVC+RNTTA+DIYRNIN+HHVTHMCCAPIVFRIILEA   +RRR G  VNV
Sbjct: 241 WGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQ KWN LP+D+QAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 IGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGWFLTG 420
           I ILTLADVDVKNLETM+SVPHDGRT GEIVLRGSSIMKGY KDPAATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEI+DRSKDVIISGGENISSVE+ETVLY HP VAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKFQLRE 540
           CAF+ VH  +    G V+EAE++EFCR N+ HYM PKKV+FVAELPKT TGKIQKF+LRE
Sbjct: 481 CAFVVVHSDS----GKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFNVSEKRVSGGNKSGRQDGGRRADYGEHVLAMSRL 579
           QAKL   SEKRVSGGNKSGR DGGR  +YGEHV+AMSRL
Sbjct: 541 QAKLLVASEKRVSGGNKSGRHDGGRLPEYGEHVMAMSRL 575

BLAST of MC04g1173 vs. ExPASy TrEMBL
Match: A0A6J1ECD9 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431279 PE=4 SV=1)

HSP 1 Score: 998 bits (2581), Expect = 0.0
Identity = 487/579 (84.11%), Postives = 524/579 (90.50%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M SLSKCD+N+ +LTPI+FL+RASA YADRTS+IYE TRFTWR T+QRC RLASSL  LN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           V+KN VVSV+APNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+LFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIPQAKDALRLLVAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVEVDDE 180
           +I +AKDALR L+A S PLP VVVIDDIDSPTG RFGELEYE L+ +G+  F  VEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGSPVNV 300
           WGIAARGGTNVCMRNTTA+DIYRNIN+HHVTHMCCAPIVFRIILEA   +RRR G  VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQ KWN LP+D+QAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 IGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGWFLTG 420
           I ILTLADVDVKNLETM+SVPHDGRT GEIVLRGSSIMKGY KDPAATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEI+DRSKDVIISGGENISSVE+ETVLY HP VAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKFQLRE 540
           CAF+ VH       G V+EAE++EFCR N+ HYM PKKVVFVAELPKT TGKIQKF+LRE
Sbjct: 481 CAFVVVHSDG----GKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFNVSEKRVSGGNKSGRQDGGRRADYGEHVLAMSRL 579
           QAKL   SEKRVSGGNKSGR DGGR+ DYG+HV+AMSRL
Sbjct: 541 QAKLLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSRL 575

BLAST of MC04g1173 vs. ExPASy TrEMBL
Match: A0A6J1JUR4 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111487678 PE=4 SV=1)

HSP 1 Score: 990 bits (2560), Expect = 0.0
Identity = 483/579 (83.42%), Postives = 521/579 (89.98%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M SLSKCD+NF +LTPI+FL+RASA YADRTS+IYE T FTWR T++RC  LASSL  LN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           V KN VVSV+APNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRHSEAK+LFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIPQAKDALRLLVAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVEVDDE 180
           +I +AKDALR L+A S PLP VVVIDDIDSPTG RFGELEYE L+ +G+  F  VEVDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGSPVNV 300
           WGIAARGGTNVC+RNTTA+DIYRNIN+HHVTHMCCAPIVFRIILEA   +RRR G  VNV
Sbjct: 241 WGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQ KWN LP+D+QAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 IGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGWFLTG 420
           I ILTLADVDVKNLETM+SVPHDGRT GEIVLRGSSIMKGY KDPAATA AF+NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEI+DRSKDVIISGGENISSVE+ETVLY HP VAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKFQLRE 540
           CAF+ VH  +    G V+EAE++EFCR N+ HYM PKKV+FVAELPKT TGKIQKF+LRE
Sbjct: 481 CAFVVVHSDS----GKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFNVSEKRVSGGNKSGRQDGGRRADYGEHVLAMSRL 579
           QAKL   SEKRVSGGNKSGR DGGR  +YGEHV+AMSRL
Sbjct: 541 QAKLLVASEKRVSGGNKSGRHDGGRLPEYGEHVMAMSRL 575

BLAST of MC04g1173 vs. ExPASy TrEMBL
Match: A0A0A0KT40 (Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G319910 PE=4 SV=1)

HSP 1 Score: 970 bits (2508), Expect = 0.0
Identity = 475/576 (82.47%), Postives = 518/576 (89.93%), Query Frame = 0

Query: 4   LSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLNVSK 63
           L KCD+N+ ALTPI+FL+RASA YADRTS+IYE TRFTW+ T++RC RLASSL  L VSK
Sbjct: 2   LLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLTVSK 61

Query: 64  NDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQYIP 123
           NDVVSV+APNVPALYEMHFAVPMAGA+LNTINTRLD+KNIA+ILRHSEAK+ FVDYQYI 
Sbjct: 62  NDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQYIQ 121

Query: 124 QAKDALRLLVAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVEVDDEWDS 183
           +AKDALRLLVAESSP+PLVVVIDDID+PTG R G LEYE LI +G+  F  VEVDDEWDS
Sbjct: 122 EAKDALRLLVAESSPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDDEWDS 181

Query: 184 IALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI 243
           IALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI
Sbjct: 182 IALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI 241

Query: 244 AARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGSPVNVLTG 303
           AARGGTN+CMRNTTA DI+RNIN+H VTHMCCAPIVF IILEA++ DRRRV  PVNVLTG
Sbjct: 242 AARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVNVLTG 301

Query: 304 GAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLKARQGIGI 363
           GAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQ KWN LP+DQQA LKARQGI I
Sbjct: 302 GAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQGISI 361

Query: 364 LTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGWFLTGDVG 423
           LTLADVDVKNL+TMESVPHDGRT GEIVLRGSS+MKGY KDPAATAAA KNGWFLTGD+G
Sbjct: 362 LTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPAATAAALKNGWFLTGDIG 421

Query: 424 VIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRWGESPCAF 483
           VI+PDGY+EI+DRSKDVIISGGENISSVE+ETVLY HP V+EAAVVAMPHPRWGESPCAF
Sbjct: 422 VIYPDGYVEIKDRSKDVIISGGENISSVEVETVLYRHPQVSEAAVVAMPHPRWGESPCAF 481

Query: 484 IAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKFQLREQAK 543
           + +  G  GG+G V+EAE+V FCR NL HYMVPKKVVFVAELPK A GK+QKF LREQAK
Sbjct: 482 VVLQDG--GGSGRVSEAEMVAFCRKNLSHYMVPKKVVFVAELPK-ANGKVQKFMLREQAK 541

Query: 544 LFNVSEKRVSGGNKSGRQDGGRRADYGEHVLAMSRL 579
              VSEKR+   +  G + G RR+DYGEHV+AMSRL
Sbjct: 542 YLEVSEKRIV--SNMGNKSGNRRSDYGEHVIAMSRL 572

BLAST of MC04g1173 vs. ExPASy TrEMBL
Match: A0A5A7TXW0 (Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold284G00160 PE=4 SV=1)

HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 482/582 (82.82%), Postives = 522/582 (89.69%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           ME L KCD+N+ ALTPI+FL+RASA YADRTS+IYE TRFTW  T++RC RLASSLR L+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           VSKN VVSV+APNVPALYEMHFAVPMAGA+LNTINTRLDVKNIA+ILRHSEAK+ FVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIPQAKDALRLLVAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVEVDDE 180
           YI +AKDALRLLVAES  +PLVVVIDDID+PTG R G LEYE LI +G+  F  VEVDDE
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGSPVNV 300
           WGIAARGGTN+CMRNTTA DI+RNIN+H VTHMCCAPIVFRIILEA++ DRRRV  PVNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQ KWN LP+DQQA LKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 IGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGWFLTG 420
           I ILTLADVDVKNL+TMESVPHDGRT GEIVLRGSS+MKGY KDP ATAAAFKNGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEI+DRSKDVIISGGENISSVE+ETVLY HP VAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKFQLRE 540
           CAF+ +HGG  GG+G V+EAE++ FCR NL HYMVPKKVVF+AELPK A GK+QKF LRE
Sbjct: 481 CAFVVLHGG--GGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLRE 540

Query: 541 QAKLFNVSEKR-VSG-GNKSGRQDGGRRADYGE-HVLAMSRL 579
           QAK   VSEKR VS  GNKSG     RR+DYG+ HV+AMSRL
Sbjct: 541 QAKYLEVSEKRRVSNLGNKSGN----RRSDYGDQHVIAMSRL 575

BLAST of MC04g1173 vs. ExPASy TrEMBL
Match: A0A1S3CIM1 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103501376 PE=4 SV=1)

HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 482/582 (82.82%), Postives = 522/582 (89.69%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           ME L KCD+N+ ALTPI+FL+RASA YADRTS+IYE TRFTW  T++RC RLASSLR L+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           VSKN VVSV+APNVPALYEMHFAVPMAGA+LNTINTRLDVKNIA+ILRHSEAK+ FVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIPQAKDALRLLVAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVEVDDE 180
           YI +AKDALRLLVAES  +PLVVVIDDID+PTG R G LEYE LI +G+  F  VEVDDE
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGSPVNV 300
           WGIAARGGTN+CMRNTTA DI+RNIN+H VTHMCCAPIVFRIILEA++ DRRRV  PVNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQ KWN LP+DQQA LKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 IGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGWFLTG 420
           I ILTLADVDVKNL+TMESVPHDGRT GEIVLRGSS+MKGY KDP ATAAAFKNGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEI+DRSKDVIISGGENISSVE+ETVLY HP VAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKFQLRE 540
           CAF+ +HGG  GG+G V+EAE++ FCR NL HYMVPKKVVF+AELPK A GK+QKF LRE
Sbjct: 481 CAFVVLHGG--GGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLRE 540

Query: 541 QAKLFNVSEKR-VSG-GNKSGRQDGGRRADYGE-HVLAMSRL 579
           QAK   VSEKR VS  GNKSG     RR+DYG+ HV+AMSRL
Sbjct: 541 QAKYLEVSEKRRVSNLGNKSGN----RRSDYGDQHVIAMSRL 575

BLAST of MC04g1173 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 705.7 bits (1820), Expect = 3.1e-203
Identity = 339/554 (61.19%), Postives = 426/554 (76.90%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M++L  C++N   LTPI+FL+RAS  Y +RTS+IY +TRFTW  T+ RC RLA+SL SLN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           +++NDVVS++APNVPA+YEMHF+VPM GAVLN INTRLD K IA+ILRH+E K+LFVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIPQAKDALRLL-VAESSPLPLVVVIDDIDSPTGIRFGELEYEDLIRNGNP----EFVAV 180
           + P  ++ LRL+   +S P P +++I++IDS T     EL+YE LIR G P         
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            V +E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVG 300
           GWT TW +AARGGTNVC+R+ TA +IY+NI +H VTHM C P VFR +LE    D+    
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 SPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKL 360
           SPV VLTGG+ PPA L++K+E LGFH+ H YGLTEATGP L CEWQ +WN LP  QQ +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGIGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNG 420
           + RQG+  LTLADVDVKN +T+ESVP DG+T GEIV++GSS+MKGYLK+P AT+ AFK+G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPR 480
           W  TGD+GVIHPDGY+EI+DRSKD+IISGGENISS+E+E VLY +  V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQK 540
           WGE+PCAF+ +  G  G     +E +++++CR N+PH+M PKKVVF  ELPK + GKI K
Sbjct: 481 WGETPCAFVVLKKGEEGLV--TSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILK 540

Query: 541 FQLREQAKLFNVSE 550
            +LR+ AK   V E
Sbjct: 541 SKLRDIAKALVVRE 552

BLAST of MC04g1173 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 700.3 bits (1806), Expect = 1.3e-201
Identity = 339/553 (61.30%), Postives = 423/553 (76.49%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M++L+ C++N   LTPI+FL+RAS  Y +RTS+IY +TRFTW  T+ RC RLA+SL SLN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           + KNDVVSV+APN PA+YEMHFAVPMAGAVLN INTRLD  +IA ILRH++ K+LF+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIPQAKDALRLLVAESSPLPL-VVVIDDIDSPTGIRFGELEYEDLIRNGNPEFVAVE--- 180
           + P A++ L+LL +E S L L V+ I +ID P  +   E +YE LI+ G P  + +    
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARMF 180

Query: 181 -VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 CIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVG 300
           GWTFTWG AARGGT+VCMR+ TA +IY+NI MH+VTHMCC P VF I+L+  S D     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRS 300

Query: 301 SPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKL 360
            PV+VLTGG+PPPA L++K++ LGF + HAYGLTEATGP L CEWQ +WN LP +QQ +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGIGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNG 420
           KARQG+ IL L +VDV+N ET ESVP DG+T GEIV++GSSIMKGYLK+P AT  AFK+G
Sbjct: 361 KARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPR 480
           W  +GDVGVIHPDG++EI+DRSKD+IISGGENISSVE+E ++Y +P V E AVVAMPHP 
Sbjct: 421 WLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFIAVHGGAHGGAGG-----VTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTAT 540
           WGE+PCAF+ +  G              E +++E+CR NLPH+M P+KVVF+ ELPK   
Sbjct: 481 WGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKNGN 540

Query: 541 GKIQKFQLREQAK 544
           GKI K +LR+ AK
Sbjct: 541 GKILKPKLRDIAK 553

BLAST of MC04g1173 vs. TAIR 10
Match: AT1G65880.1 (benzoyloxyglucosinolate 1 )

HSP 1 Score: 695.3 bits (1793), Expect = 4.2e-200
Identity = 344/554 (62.09%), Postives = 419/554 (75.63%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           M+ L+ C++N   LTP++FL+RAS  Y +RTS+IY +TRFTW  T+ RC RLA+SL SLN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           +SKNDVVSV+APN PALYEMHFAVPMAGAVLN INTRLD  +IA ILRH++ K+LF+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIPQAKDALRLLVAESSPLPL-VVVIDDIDSPTGIRFGELEYEDLIRNG--NPEFVA--V 180
           +   A+++L LL +E S L L V+ I + D P    F EL+YE LI+ G   P  VA   
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 EVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVG 300
           GWTFTWG AARGGT+VCMR+ TA +IY+NI MH+VTHMCC P VF I+L+  S D     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 SPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKL 360
            PV+VLTGG+PPPA L++K++ LGF + HAYG TEATGP L CEWQ +WN LP +QQ +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGIGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNG 420
           KARQGI IL LADVDVKN ET +S P DG+T GEI+++GSSIMKGYLK+P AT  AFK+G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPR 480
           W  TGDVGVIHPDG++EI+DRSKD+IISGGENISSVE+E VLY +P V E AVVAMPHP 
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFIAVHGGA------HGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTA 540
           WGE+PCAF+ +                  E  ++E+CR NLPH+M P+KVVF+ ELPK  
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540

Query: 541 TGKIQKFQLREQAK 544
            GKI K +LR+ AK
Sbjct: 541 NGKILKPKLRDIAK 554

BLAST of MC04g1173 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 586.6 bits (1511), Expect = 2.1e-167
Identity = 284/555 (51.17%), Postives = 395/555 (71.17%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIYERTRFTWRNTFQRCSRLASSLRSLN 60
           ME   K  +N+  LTPISFL R++  YADR S++Y   ++TWR T  RC R+AS+L  L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  VSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDYQ 120
           +S  DVVSV+APNVPA+ E+HF VPMAGA+L T+N R D   +AV+LRHS  KV+F D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YIPQAKDALRLLVAESSPLPLVVVIDD--IDSPTGIRFGE--LEYEDLIRNGNPEFVAVE 180
           ++  A+ A  +L  +   +P++V+I +    S +  +  E  +EYED++  G  +F  + 
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRRRVGS 300
           W   WG+ A GGTN+C+RN TA  I+ NI+ H VTHM  AP +  +I+ A  ++++ +  
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 PVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQAKLK 360
            V+ +TG APPPA ++ KME LGF + H+YGLTE  GP  +C W+ +W+SLP ++QAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGIGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAFKNGW 420
           ARQG+  L L ++ VK+  TM ++P DG T GE+V RG+++M GYLK+P AT  AFK GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMPHPRW 480
           F +GD+GV HPDGY+E++DRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGKIQKF 540
           GE+ CAF+ +  G+   A      E++ +CR  LPHYM P+ +VF  +LPKT+TGK+QKF
Sbjct: 483 GETACAFVKLKDGSKASA-----EELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKF 542

Query: 541 QLREQAK-LFNVSEK 551
            LR +AK L ++S+K
Sbjct: 543 VLRTKAKALVSLSKK 551

BLAST of MC04g1173 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 578.9 bits (1491), Expect = 4.4e-165
Identity = 290/561 (51.69%), Postives = 390/561 (69.52%), Query Frame = 0

Query: 1   MESLSKCDSNFCALTPISFLRRASASYADRTSVIY-ERTRFTWRNTFQRCSRLASSLRSL 60
           ME +  C +N   LTPI FL RA+  Y D TS++Y   T +TWR T  RC R+ASSL S+
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  NVSKNDVVSVIAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAVILRHSEAKVLFVDY 120
            + ++DVVSV++PN PA+YE+ FAVPM+GA+LN INTRLD + ++V+LRH E+K+LFVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 QYIPQAKDALRLLVAESSPLPLVVVIDDIDSPTGI-------RFGELEYEDLIRNGNPEF 180
             +  A +A+ ++  +    P++VVI D +   G+       +F    Y+DLI  G+P F
Sbjct: 121 FSVDLAVEAVSMMTTDP---PILVVIADKEEEGGVADVADLSKF-SYTYDDLIERGDPGF 180

Query: 181 VAVEVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMF 240
             +  + EWD + LNYTSGTTSAPKGVV+ HRG F+ ++  ++ W +   PVYLWTLP+F
Sbjct: 181 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIF 240

Query: 241 HCNGWTFTWGIAARGGTNVCMRNTTAYDIYRNINMHHVTHMCCAPIVFRIILEAESADRR 300
           H NGWT  WGIAA GGTNVC+R   A  IYR I  H VTHMC AP+V  ++  + + + +
Sbjct: 241 HSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNML--SATQESQ 300

Query: 301 RVGSPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQHKWNSLPSDQQ 360
            +  PVN+LT G+PPPA +L + E++GF I+H YGLTE  G  + C W+ KWN LP+  +
Sbjct: 301 PLNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDR 360

Query: 361 AKLKARQGIGILTLADVDVKNLETMESVPHDGRTAGEIVLRGSSIMKGYLKDPAATAAAF 420
           A+LKARQG+  +   ++DV + E+  SV  +G T GEIV+RGSS+M GYLKDP  T  A 
Sbjct: 361 ARLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL 420

Query: 421 KNGWFLTGDVGVIHPDGYLEIRDRSKDVIISGGENISSVELETVLYGHPGVAEAAVVAMP 480
           KNGWF TGDVGVIH DGYLEI+DRSKD+II+GGEN+SSVE+ETVLY  P V E AVVA P
Sbjct: 421 KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARP 480

Query: 481 HPRWGESPCAFIAVHGGAHGGAGGVTEAEIVEFCRGNLPHYMVPKKVVFVAELPKTATGK 540
              WGE+PCAF+++    +G +G  TE E++E+CR  +P YMVPK V F+ ELPK++TGK
Sbjct: 481 DEFWGETPCAFVSL---KNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGK 540

Query: 541 IQKFQLREQAKLFNVSEKRVS 554
           + KF LR+ AK   + +K +S
Sbjct: 541 VTKFVLRDIAK--KMGDKTIS 550