MC08g1153 (gene) Bitter gourd (Dali-11) v1

BLAST of MC08g1153 vs. ExPASy Swiss-Prot
Match: B9DGD6 (Acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal OS=Arabidopsis thaliana OX=3702 GN=ACS PE=1 SV=1)

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 562/710 (79.15%), Postives = 626/710 (88.17%), Query Frame = 0

Query: 91  MGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQK 150
           M    L   + ++Q+ S A  KI +LNA VLGESLASE++ L+ PS +FS +A VSS Q+
Sbjct: 51  MSSNSLRHVESMSQLPSGAG-KISQLNAVVLGESLASEENDLVFPSKEFSGQALVSSPQQ 110

Query: 151 YLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICY 210
           Y+EM+KRS++DPA FWSDIA SEF+WK +W   V+SENLDVRKG I IEWFKGGITNICY
Sbjct: 111 YMEMHKRSMDDPAAFWSDIA-SEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICY 170

Query: 211 NCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIY 270
           NCLD+N+EAGLGDK A++WEGNE G D +LTY+ELL +VCQLANYLKDNGVKKGDAVVIY
Sbjct: 171 NCLDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIY 230

Query: 271 MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL 330
           +PMLMELPIAMLACARIGA+HSVVFAGFS++SLAQRI+DCKP +++TCNAVKRG K I+L
Sbjct: 231 LPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINL 290

Query: 331 KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEW 390
           K IVDAAL +S+++ VSV  CLTY+N  A  RE+T W+ GRD+WWQDV+  YPT+C VEW
Sbjct: 291 KAIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEW 350

Query: 391 VDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITG 450
           VDAE PLFLLYTSGSTGKPKGVLHTTGGYM+YTATTFKYAFDYK  DVYWCTADCGWITG
Sbjct: 351 VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITG 410

Query: 451 HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHV 510
           HSYVTYGP+LNGA+VVV+EGAPNYPDPGRCWDIVDK+KV+IFYTAPTLVRSLMR+ ++ V
Sbjct: 411 HSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFV 470

Query: 511 TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ 570
           TR+SRKSLRVLGSVGEPINPSAWRWFFNVVG+SRCPISDTWWQTETGGFMITPLPGAWPQ
Sbjct: 471 TRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQ 530

Query: 571 KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPF 630
           KPGSATFPFFGVQPV+VDEKGNEIEGECSGYLC+K SWP AFRTL+ D ERYE TYFKPF
Sbjct: 531 KPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPF 590

Query: 631 PGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 690
            GYYF+GDGC                SRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL
Sbjct: 591 AGYYFSGDGC----------------SRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 650

Query: 691 VSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRI 750
           V HP CAEAAVVGIEHEVKGQGIYAFVTL++GV YSEELRKSL+L VR QIGAFAAPDRI
Sbjct: 651 VLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRI 710

Query: 751 HWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELAD 801
           HWAPGLPKTRSGKIMRRILRKIAS+QL+ELGDTSTLADP VVDQLI LAD
Sbjct: 711 HWAPGLPKTRSGKIMRRILRKIASRQLEELGDTSTLADPSVVDQLIALAD 742

BLAST of MC08g1153 vs. ExPASy Swiss-Prot
Match: Q9NR19 (Acetyl-coenzyme A synthetase, cytoplasmic OS=Homo sapiens OX=9606 GN=ACSS2 PE=1 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 7.6e-229
Identity = 386/680 (56.76%), Postives = 473/680 (69.56%), Query Frame = 0

Query: 135 PSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKG 194
           P  + S  AHV S Q+Y E+++RS+E+P  FW DIA  EF+WK          N DV KG
Sbjct: 31  PPPEVSRSAHVPSLQRYRELHRRSVEEPREFWGDIA-KEFYWKTPCPGPFLRYNFDVTKG 90

Query: 195 EIKIEWFKGGITNICYNCLDRNI-EAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLA 254
           +I IEW KG  TNICYN LDRN+ E  LGDKVA YWEGNEPG    +TY +LL +VCQ +
Sbjct: 91  KIFIEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTQITYHQLLVQVCQFS 150

Query: 255 NYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPK 314
           N L+  G++KGD V IYMPM+ EL +AMLACARIGA+HS+VFAGFSSESL +RI+D    
Sbjct: 151 NVLRKQGIQKGDRVAIYMPMIPELVVAMLACARIGALHSIVFAGFSSESLCERILDSSCS 210

Query: 315 IVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYEN--------------ESA 374
           ++IT +A  RG K+++LK++ D AL +  +    V  C+  ++                 
Sbjct: 211 LLITTDAFYRGEKLVNLKELADEALQKCQEKGFPVRCCIVVKHLGRAELGMGDSTSQSPP 270

Query: 375 LKRE----STTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHT 434
           +KR       +W +G D+WW +++      C  EW DAE PLF+LYTSGSTGKPKGV+HT
Sbjct: 271 IKRSCPDVQISWNQGIDLWWHELMQEAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHT 330

Query: 435 TGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYP 494
            GGYM+Y ATTFKY FD+   DV+WCTAD GWITGHSYVTYGPL NGA+ V++EG P YP
Sbjct: 331 VGGYMLYVATTFKYVFDFHAEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYP 390

Query: 495 DPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRW 554
           D  R W IVDK+KVT FYTAPT +R LM+ G+E VT++SR SL+VLG+VGEPINP AW W
Sbjct: 391 DVNRLWSIVDKYKVTKFYTAPTAIRLLMKFGDEPVTKHSRASLQVLGTVGEPINPEAWLW 450

Query: 555 FFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIE 614
           +  VVG  RCPI DT+WQTETGG M+TPLPGA P KPGSATFPFFGV P +++E G E+E
Sbjct: 451 YHRVVGAQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSATFPFFGVAPAILNESGEELE 510

Query: 615 GECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTL 674
           GE  GYL  K  WP   RT+Y + ER+E TYFK FPGYY TGDGC               
Sbjct: 511 GEAEGYLVFKQPWPGIMRTVYGNHERFETTYFKKFPGYYVTGDGC--------------- 570

Query: 675 SSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYA 734
             RD+DGYYW+TGR+DD++NVSGH + TAEVESALV H   AEAAVVG  H VKG+ +Y 
Sbjct: 571 -QRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYC 630

Query: 735 FVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASK 794
           FVTL DG ++S +L + L   +R++IG  A PD I  APGLPKTRSGKIMRR+LRKIA  
Sbjct: 631 FVTLCDGHTFSPKLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQN 690

Query: 795 QLDELGDTSTLADPGVVDQL 796
             D LGD ST+ADP V+  L
Sbjct: 691 DHD-LGDMSTVADPSVISHL 692

BLAST of MC08g1153 vs. ExPASy Swiss-Prot
Match: Q9QXG4 (Acetyl-coenzyme A synthetase, cytoplasmic OS=Mus musculus OX=10090 GN=Acss2 PE=1 SV=2)

HSP 1 Score: 791.6 bits (2043), Expect = 8.4e-228
Identity = 384/680 (56.47%), Postives = 475/680 (69.85%), Query Frame = 0

Query: 135 PSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKG 194
           P  +    AHV S Q+Y E+++RS+E+P  FW +IA  EF+WK          N DV KG
Sbjct: 31  PPPEVRRSAHVPSLQRYRELHRRSVEEPREFWGNIA-KEFYWKTACPGPFLQYNFDVTKG 90

Query: 195 EIKIEWFKGGITNICYNCLDRNI-EAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLA 254
           +I  EW KG  TNICYN LDRN+ E  LGDKVA YWEGNEPG    +TY ELL +VCQ +
Sbjct: 91  KIFTEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRELLVQVCQFS 150

Query: 255 NYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPK 314
           N L+  G++KGD V IYMPM++EL +AMLACAR+GA+HS+VFAGFS+ESL +RI+D    
Sbjct: 151 NVLRKQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCC 210

Query: 315 IVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYE---------NES-----A 374
           ++IT +A  RG K+++LK++ D +L +  +    V  C+  +         N+S      
Sbjct: 211 LLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPP 270

Query: 375 LKRE----STTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHT 434
           +KR        W EG D+WW +++      C  EW DAE PLF+LYTSGSTGKPKGV+HT
Sbjct: 271 VKRPCPDVQICWNEGVDLWWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHT 330

Query: 435 TGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYP 494
            GGYM+Y ATTFKY FD+ P DV+WCTAD GWITGHSYVTYGPL NGA+ V++EG P YP
Sbjct: 331 IGGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYP 390

Query: 495 DPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRW 554
           D GR W IVDK+KVT FYTAPT +R LM+ G++ VT++SR SL+VLG+VGEPINP AW W
Sbjct: 391 DEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLW 450

Query: 555 FFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIE 614
           +  VVG  RCPI DT+WQTETGG M+TPLPGA P KPGSA+FPFFGV P +++E G E+E
Sbjct: 451 YHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELE 510

Query: 615 GECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTL 674
           GE  GYL  K  WP   RT+Y +  R+E TYFK FPGYY TGDGC               
Sbjct: 511 GEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGC--------------- 570

Query: 675 SSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYA 734
             RD+DGYYW+TGR+DD++NVSGH + TAEVESALV H   AEAAVVG  H VKG+ +Y 
Sbjct: 571 -RRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYC 630

Query: 735 FVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASK 794
           FVTL DG ++S  L + L   +R++IG  A PD I  APGLPKTRSGKIMRR+LRKIA  
Sbjct: 631 FVTLCDGHTFSPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQN 690

Query: 795 QLDELGDTSTLADPGVVDQL 796
             D LGDTST+ADP V++ L
Sbjct: 691 DHD-LGDTSTVADPSVINHL 692

BLAST of MC08g1153 vs. ExPASy Swiss-Prot
Match: A0L576 (Acetyl-coenzyme A synthetase OS=Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1) OX=156889 GN=acsA PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 6.3e-223
Identity = 374/668 (55.99%), Postives = 472/668 (70.66%), Query Frame = 0

Query: 130 DQLILPSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENL 189
           D  + P D   +   + + ++Y EMYKRSI+DPA FW++ A +   W  +WD ++   + 
Sbjct: 3   DVKVYPVDPNVAANALINEEQYQEMYKRSIDDPAAFWAEQAEARIDWFKKWDNVL---DF 62

Query: 190 DVRKGEIKIEWFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKV 249
           D+RKG IK  WF+G   N+ YNC+DR++E   GDKVA+ WEG+ P  D  +TY EL   V
Sbjct: 63  DLRKGHIK--WFEGAKLNVSYNCIDRHLEK-RGDKVAILWEGDNPAADRKITYRELSALV 122

Query: 250 CQLANYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIID 309
            + AN LK  GVKKGD V IYMPM+ E  +AMLAC RIGA+HS+VF GFS ++L  RIID
Sbjct: 123 NKFANALKARGVKKGDRVCIYMPMIPEAAVAMLACTRIGAVHSIVFGGFSPDALRDRIID 182

Query: 310 CKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKE 369
            + ++VIT +   RGSK I L+  V+ AL +      +V TC   + ++ L+ E   W E
Sbjct: 183 AECRVVITADGGMRGSKKIPLRKNVETALAQCP----NVHTCFVVQ-DTQLEIE---WTE 242

Query: 370 GRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKY 429
           GRD+W+ + +      C  E +DAE PLF+LYTSGSTGKPKGVLHTTGGY++Y + T +Y
Sbjct: 243 GRDVWYHEAIAAASADCPPEEMDAEDPLFVLYTSGSTGKPKGVLHTTGGYLLYASITHQY 302

Query: 430 AFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKV 489
            FDY   D+YWCTAD GW+TGH+Y+ YGPL NGA+ +++EG P YPD  R W +VDKH+V
Sbjct: 303 IFDYHEDDIYWCTADVGWVTGHTYIVYGPLANGATTLMFEGVPTYPDASRFWQVVDKHQV 362

Query: 490 TIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISD 549
           +IFYTAPT +RS+M +G+  V   SR+SLR+LGSVGEPINP AW W+++VVGE RCPI D
Sbjct: 363 SIFYTAPTAIRSIMSQGDALVKATSRQSLRILGSVGEPINPEAWEWYYHVVGEGRCPIVD 422

Query: 550 TWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWP 609
           TWWQTETGG +ITPLPGA P KPGSAT PFFGV P ++  +G E+EG C G L L   WP
Sbjct: 423 TWWQTETGGILITPLPGATPLKPGSATRPFFGVNPKVLSNEGKEMEGPCEGILVLDHPWP 482

Query: 610 AAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYWLTGR 669
              RT+Y D +R+  TYF  +PGYYF GDGC                 RD+DGYYW+TGR
Sbjct: 483 GMMRTVYGDHDRFINTYFANYPGYYFCGDGC----------------RRDEDGYYWITGR 542

Query: 670 VDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEEL 729
           VDDVINVSGHR+GTAEVESALV H   AEAAVVG+ HE+KGQGIYA+VTL+ GV  S+ L
Sbjct: 543 VDDVINVSGHRLGTAEVESALVLHDQVAEAAVVGMPHEIKGQGIYAYVTLMSGVEPSDAL 602

Query: 730 RKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADP 789
           +  L+  VRK+IG  A+ D I +APGLPKTRSGKIMRRILRKIA+ ++D LGDTSTLADP
Sbjct: 603 KTELVKLVRKEIGPIASLDVIQFAPGLPKTRSGKIMRRILRKIAAHEVDTLGDTSTLADP 640

Query: 790 GVVDQLIE 798
            VV  LI+
Sbjct: 663 SVVQNLID 640

BLAST of MC08g1153 vs. ExPASy Swiss-Prot
Match: Q04P35 (Acetyl-coenzyme A synthetase OS=Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197) OX=355277 GN=acsA PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 1.1e-222
Identity = 381/672 (56.70%), Postives = 473/672 (70.39%), Query Frame = 0

Query: 130 DQLILPSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENL 189
           ++++LPS +F   ++++  + Y  +YK SIE+P  FW+  A +   W  +W +++   N 
Sbjct: 4   ERIVLPSAEFKKNSNITL-KDYKSLYKESIENPNKFWAKEA-NRLTWFKKWTKVL---NH 63

Query: 190 DVRKGEIKIEWFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKV 249
           D +    K+EWFKGG  N+ YNCLDR+I   L +K AL WEG+ P     LTY ++  +V
Sbjct: 64  DFKNA--KVEWFKGGKLNVSYNCLDRHISTPLKNKAALIWEGDNPSESKVLTYYDVYREV 123

Query: 250 CQLANYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIID 309
            + AN LK  GVKKGD V++Y+PM+ EL I +LAC RIGAIHSVVF GFS E+L  RI D
Sbjct: 124 NRFANVLKKYGVKKGDRVLVYLPMIPELAITILACTRIGAIHSVVFGGFSPEALQSRIDD 183

Query: 310 CKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKE 369
           CKPK+++T +   RG K I LK  VD AL +S ++  +V       NES L      WK+
Sbjct: 184 CKPKLIVTADGGFRGGKPIELKRNVDIALEKSKEDVKTVIVVRRTGNESGL-----VWKD 243

Query: 370 GRDIWWQDVV--PTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTF 429
           GRD W+  ++  P     C  E +DAE PLF+LYTSGSTGKPKGVLHTTGGY++    TF
Sbjct: 244 GRDYWYHFLIGDPDLSPYCKPESMDAEDPLFILYTSGSTGKPKGVLHTTGGYLLGVNLTF 303

Query: 430 KYAFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKH 489
            Y FD KP D YWCTAD GW+TGHSY+ YGPL NGAS V++EG P+YPD GR WD++DK+
Sbjct: 304 HYVFDIKPEDTYWCTADIGWVTGHSYLVYGPLSNGASSVMFEGVPSYPDAGRFWDVIDKY 363

Query: 490 KVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPI 549
            V IFYTAPT +R+LMREG  HV + +  SLR+LGSVGEPINP AW W+F ++G+ +CPI
Sbjct: 364 GVNIFYTAPTAIRALMREGLTHVQKRNLSSLRLLGSVGEPINPEAWEWYFKIIGKEKCPI 423

Query: 550 SDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEI--EGECSGYLCLK 609
            DTWWQTETG  MIT LPGA PQKPGSAT PFFGVQPVLVD  G EI  +GE SG LC+K
Sbjct: 424 VDTWWQTETGSIMITALPGAIPQKPGSATLPFFGVQPVLVDNDGKEINDKGEVSGNLCIK 483

Query: 610 SSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYW 669
           S WP+  R +Y D +R+  TYF  F GYYFTGDG                + RDKDGYYW
Sbjct: 484 SPWPSMMRGVYGDSKRFFDTYFSQFKGYYFTGDG----------------ARRDKDGYYW 543

Query: 670 LTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSY 729
           +TGRVDDVINVSGHRIG+AEVESALV +   AEAAVVG  H++KGQGIYA+VT+ +G++ 
Sbjct: 544 ITGRVDDVINVSGHRIGSAEVESALVENRSVAEAAVVGFPHDIKGQGIYAYVTVKEGIAT 603

Query: 730 SEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTST 789
           ++ L+K L+  V K IG  A PD IHWAP LPKTRSGKIMRRILRKIAS + + LGDTST
Sbjct: 604 NDTLKKELVAIVEKVIGKIARPDVIHWAPSLPKTRSGKIMRRILRKIASGEFEGLGDTST 647

Query: 790 LADPGVVDQLIE 798
           LADP VV +LIE
Sbjct: 664 LADPSVVQKLIE 647

BLAST of MC08g1153 vs. NCBI nr
Match: XP_022148217.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X1 [Momordica charantia])

HSP 1 Score: 1621 bits (4197), Expect = 0.0
Identity = 785/801 (98.00%), Postives = 785/801 (98.00%), Query Frame = 0

Query: 1   VATSSCFIRPFPANPTFLFPSASSANNSVHKIHNWKQPNYKKRQVNTFHSYYYFNSCLRP 60
           VATSSCFIRPFPANPTFLFPSASSANNSVHKIHNWKQPNYKKRQVNTFHSYYYFNSCLRP
Sbjct: 6   VATSSCFIRPFPANPTFLFPSASSANNSVHKIHNWKQPNYKKRQVNTFHSYYYFNSCLRP 65

Query: 61  SCARSFGFTRSSSGFCCSFSRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATV 120
           SCARSFGFTRSSSGFCCSFSRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATV
Sbjct: 66  SCARSFGFTRSSSGFCCSFSRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATV 125

Query: 121 LGESLASEDDQLILPSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRW 180
           LGESLASEDDQLILPSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRW
Sbjct: 126 LGESLASEDDQLILPSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRW 185

Query: 181 DQLVYSENLDVRKGEIKIEWFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTL 240
           DQLVYSENLDVRKGEIKIEWFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTL
Sbjct: 186 DQLVYSENLDVRKGEIKIEWFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTL 245

Query: 241 TYTELLHKVCQLANYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSS 300
           TYTELLHKVCQLANYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSS
Sbjct: 246 TYTELLHKVCQLANYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSS 305

Query: 301 ESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESAL 360
           ESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESAL
Sbjct: 306 ESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESAL 365

Query: 361 KRESTTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYM 420
           KRESTTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYM
Sbjct: 366 KRESTTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYM 425

Query: 421 VYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRC 480
           VYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRC
Sbjct: 426 VYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRC 485

Query: 481 WDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVV 540
           WDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVV
Sbjct: 486 WDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVV 545

Query: 541 GESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSG 600
           GESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSG
Sbjct: 546 GESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSG 605

Query: 601 YLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTLSSRDK 660
           YLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTR                DK
Sbjct: 606 YLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTR----------------DK 665

Query: 661 DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLV 720
           DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLV
Sbjct: 666 DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLV 725

Query: 721 DGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDEL 780
           DGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDEL
Sbjct: 726 DGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDEL 785

Query: 781 GDTSTLADPGVVDQLIELADC 801
           GDTSTLADPGVVDQLIELADC
Sbjct: 786 GDTSTLADPGVVDQLIELADC 790

BLAST of MC08g1153 vs. NCBI nr
Match: XP_022148218.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X2 [Momordica charantia])

HSP 1 Score: 1454 bits (3765), Expect = 0.0
Identity = 706/722 (97.78%), Postives = 706/722 (97.78%), Query Frame = 0

Query: 80  SRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQF 139
           SRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQF
Sbjct: 5   SRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQF 64

Query: 140 SSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIE 199
           SSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIE
Sbjct: 65  SSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIE 124

Query: 200 WFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDN 259
           WFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDN
Sbjct: 125 WFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDN 184

Query: 260 GVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN 319
           GVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN
Sbjct: 185 GVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN 244

Query: 320 AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVV 379
           AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVV
Sbjct: 245 AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVV 304

Query: 380 PTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVY 439
           PTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVY
Sbjct: 305 PTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVY 364

Query: 440 WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLV 499
           WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLV
Sbjct: 365 WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLV 424

Query: 500 RSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF 559
           RSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF
Sbjct: 425 RSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF 484

Query: 560 MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ 619
           MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ
Sbjct: 485 MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ 544

Query: 620 ERYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDVINVSGH 679
           ERYEMTYFKPFPGYYFTGDGCTR                DKDGYYWLTGRVDDVINVSGH
Sbjct: 545 ERYEMTYFKPFPGYYFTGDGCTR----------------DKDGYYWLTGRVDDVINVSGH 604

Query: 680 RIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRK 739
           RIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRK
Sbjct: 605 RIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRK 664

Query: 740 QIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELA 799
           QIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELA
Sbjct: 665 QIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELA 710

Query: 800 DC 801
           DC
Sbjct: 725 DC 710

BLAST of MC08g1153 vs. NCBI nr
Match: XP_022148219.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X3 [Momordica charantia])

HSP 1 Score: 1453 bits (3762), Expect = 0.0
Identity = 705/721 (97.78%), Postives = 705/721 (97.78%), Query Frame = 0

Query: 81  RTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFS 140
           RTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFS
Sbjct: 5   RTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFS 64

Query: 141 SRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEW 200
           SRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEW
Sbjct: 65  SRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEW 124

Query: 201 FKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNG 260
           FKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNG
Sbjct: 125 FKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNG 184

Query: 261 VKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA 320
           VKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA
Sbjct: 185 VKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA 244

Query: 321 VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVP 380
           VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVP
Sbjct: 245 VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVP 304

Query: 381 TYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYW 440
           TYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYW
Sbjct: 305 TYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYW 364

Query: 441 CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVR 500
           CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVR
Sbjct: 365 CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVR 424

Query: 501 SLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM 560
           SLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM
Sbjct: 425 SLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM 484

Query: 561 ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE 620
           ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE
Sbjct: 485 ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE 544

Query: 621 RYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDVINVSGHR 680
           RYEMTYFKPFPGYYFTGDGCTR                DKDGYYWLTGRVDDVINVSGHR
Sbjct: 545 RYEMTYFKPFPGYYFTGDGCTR----------------DKDGYYWLTGRVDDVINVSGHR 604

Query: 681 IGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQ 740
           IGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQ
Sbjct: 605 IGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQ 664

Query: 741 IGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELAD 800
           IGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELAD
Sbjct: 665 IGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELAD 709

BLAST of MC08g1153 vs. NCBI nr
Match: XP_022148220.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X4 [Momordica charantia] >XP_022148222.1 acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X4 [Momordica charantia] >XP_022148223.1 acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X4 [Momordica charantia])

HSP 1 Score: 1435 bits (3715), Expect = 0.0
Identity = 695/711 (97.75%), Postives = 695/711 (97.75%), Query Frame = 0

Query: 91  MGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQK 150
           MGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQK
Sbjct: 1   MGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQK 60

Query: 151 YLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICY 210
           YLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICY
Sbjct: 61  YLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICY 120

Query: 211 NCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIY 270
           NCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIY
Sbjct: 121 NCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIY 180

Query: 271 MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL 330
           MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL
Sbjct: 181 MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL 240

Query: 331 KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEW 390
           KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEW
Sbjct: 241 KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEW 300

Query: 391 VDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITG 450
           VDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITG
Sbjct: 301 VDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITG 360

Query: 451 HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHV 510
           HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHV
Sbjct: 361 HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHV 420

Query: 511 TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ 570
           TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ
Sbjct: 421 TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ 480

Query: 571 KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPF 630
           KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPF
Sbjct: 481 KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPF 540

Query: 631 PGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 690
           PGYYFTGDGCTR                DKDGYYWLTGRVDDVINVSGHRIGTAEVESAL
Sbjct: 541 PGYYFTGDGCTR----------------DKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 600

Query: 691 VSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRI 750
           VSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRI
Sbjct: 601 VSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRI 660

Query: 751 HWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELADC 801
           HWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELADC
Sbjct: 661 HWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELADC 695

BLAST of MC08g1153 vs. NCBI nr
Match: XP_038899318.1 (acetyl-coenzyme A synthetase, chloroplastic/glyoxysomal isoform X1 [Benincasa hispida])

HSP 1 Score: 1381 bits (3574), Expect = 0.0
Identity = 689/824 (83.62%), Postives = 729/824 (88.47%), Query Frame = 0

Query: 1   VATSSC-FIRPFPAN--PTFLFPSASSANNSVHKIHNWKQPNYKKRQVNTFHSYYYFN-- 60
           +  SSC   RPFPA+  P  LFPSA++A  S +   N +  N K+R +N+FH+Y YFN  
Sbjct: 6   IVISSCNAARPFPASSIPGILFPSAAAAIKSDNPNPNLR--NCKERHINSFHNYLYFNIT 65

Query: 61  ------SCL-RPSCARSFGFTRSSSGFCCSFSRTSG-----------EQAQVVMGFTDLT 120
                 SCL RP  A SF  + S SGF  S +RT G           ++   + GFTD+ 
Sbjct: 66  STATSSSCLLRPPSAGSF-LSSSLSGFSSSLNRTRGGDGDGDGDGRRDKNLSMGGFTDVA 125

Query: 121 APDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQKYLEMYKR 180
           A      +DSSAARKIPRLNA VLGESLA+EDDQLIL SDQFSS AH+SS+ +YLEMYKR
Sbjct: 126 A------LDSSAARKIPRLNAAVLGESLATEDDQLILSSDQFSSNAHISSYHQYLEMYKR 185

Query: 181 SIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICYNCLDRNI 240
           SIEDPAGFWSDIASSEFFWK +WDQ VYSENLDVRKGEIKIEWFKGGITNICYNCLDRNI
Sbjct: 186 SIEDPAGFWSDIASSEFFWKQKWDQQVYSENLDVRKGEIKIEWFKGGITNICYNCLDRNI 245

Query: 241 EAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIYMPMLMEL 300
           EAGLGDKVA+YWEGNEPGFDGTLTY ELLHKVCQLAN+LKDNGV+KGDAVVIYMPMLMEL
Sbjct: 246 EAGLGDKVAIYWEGNEPGFDGTLTYIELLHKVCQLANFLKDNGVRKGDAVVIYMPMLMEL 305

Query: 301 PIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAA 360
           PIAMLACARIGA+HSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVI+LKDI+DAA
Sbjct: 306 PIAMLACARIGAVHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIYLKDIIDAA 365

Query: 361 LTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPL 420
           L ESAQN VSVATCLTYEN+SALKREST WKEGRDIWWQDVVPTYPTTCTVEWVDAE+PL
Sbjct: 366 LIESAQNAVSVATCLTYENKSALKRESTKWKEGRDIWWQDVVPTYPTTCTVEWVDAEYPL 425

Query: 421 FLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTYG 480
           FLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKP DVYWCTADCGWITGHSYVTYG
Sbjct: 426 FLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYVTYG 485

Query: 481 PLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKS 540
           PLLNGASV+V+EGAPNYPDPGRCWDIVDK+KVTIFYTAPTLVRSLMREGNEHVTRY RKS
Sbjct: 486 PLLNGASVIVFEGAPNYPDPGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYLRKS 545

Query: 541 LRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATF 600
           LRVLGSVGEPINPSAWRWF+NVVGESRCPISDTWWQTETGGFMITPLPGAW QKPGSATF
Sbjct: 546 LRVLGSVGEPINPSAWRWFYNVVGESRCPISDTWWQTETGGFMITPLPGAWSQKPGSATF 605

Query: 601 PFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTG 660
           PFFGVQPVLVDEKGNEIEGECSGYLCLKSSWP+AFRTLYNDQERYE TYFKPFPGYYF+G
Sbjct: 606 PFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPSAFRTLYNDQERYETTYFKPFPGYYFSG 665

Query: 661 DGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCA 720
           DGCTR                DKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHP CA
Sbjct: 666 DGCTR----------------DKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCA 725

Query: 721 EAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLP 780
           EAAVVG+EHEVKGQGIYAFVTLVDGVSYSEELRKSLI+TVRKQIGAFAAPD+IHWAPGLP
Sbjct: 726 EAAVVGVEHEVKGQGIYAFVTLVDGVSYSEELRKSLIITVRKQIGAFAAPDKIHWAPGLP 785

Query: 781 KTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELADC 801
           KTRSGKIMRRILRKIASKQL+ELGD STLADPGVVDQLIELADC
Sbjct: 786 KTRSGKIMRRILRKIASKQLEELGDISTLADPGVVDQLIELADC 804

BLAST of MC08g1153 vs. ExPASy TrEMBL
Match: A0A6J1D4G7 (Acetate--CoA ligase OS=Momordica charantia OX=3673 GN=LOC111016935 PE=3 SV=1)

HSP 1 Score: 1621 bits (4197), Expect = 0.0
Identity = 785/801 (98.00%), Postives = 785/801 (98.00%), Query Frame = 0

Query: 1   VATSSCFIRPFPANPTFLFPSASSANNSVHKIHNWKQPNYKKRQVNTFHSYYYFNSCLRP 60
           VATSSCFIRPFPANPTFLFPSASSANNSVHKIHNWKQPNYKKRQVNTFHSYYYFNSCLRP
Sbjct: 6   VATSSCFIRPFPANPTFLFPSASSANNSVHKIHNWKQPNYKKRQVNTFHSYYYFNSCLRP 65

Query: 61  SCARSFGFTRSSSGFCCSFSRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATV 120
           SCARSFGFTRSSSGFCCSFSRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATV
Sbjct: 66  SCARSFGFTRSSSGFCCSFSRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATV 125

Query: 121 LGESLASEDDQLILPSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRW 180
           LGESLASEDDQLILPSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRW
Sbjct: 126 LGESLASEDDQLILPSDQFSSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRW 185

Query: 181 DQLVYSENLDVRKGEIKIEWFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTL 240
           DQLVYSENLDVRKGEIKIEWFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTL
Sbjct: 186 DQLVYSENLDVRKGEIKIEWFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTL 245

Query: 241 TYTELLHKVCQLANYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSS 300
           TYTELLHKVCQLANYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSS
Sbjct: 246 TYTELLHKVCQLANYLKDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSS 305

Query: 301 ESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESAL 360
           ESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESAL
Sbjct: 306 ESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESAL 365

Query: 361 KRESTTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYM 420
           KRESTTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYM
Sbjct: 366 KRESTTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYM 425

Query: 421 VYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRC 480
           VYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRC
Sbjct: 426 VYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRC 485

Query: 481 WDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVV 540
           WDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVV
Sbjct: 486 WDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVV 545

Query: 541 GESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSG 600
           GESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSG
Sbjct: 546 GESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSG 605

Query: 601 YLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTLSSRDK 660
           YLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTR                DK
Sbjct: 606 YLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTR----------------DK 665

Query: 661 DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLV 720
           DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLV
Sbjct: 666 DGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLV 725

Query: 721 DGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDEL 780
           DGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDEL
Sbjct: 726 DGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDEL 785

Query: 781 GDTSTLADPGVVDQLIELADC 801
           GDTSTLADPGVVDQLIELADC
Sbjct: 786 GDTSTLADPGVVDQLIELADC 790

BLAST of MC08g1153 vs. ExPASy TrEMBL
Match: A0A6J1D3H3 (Acetate--CoA ligase OS=Momordica charantia OX=3673 GN=LOC111016935 PE=3 SV=1)

HSP 1 Score: 1454 bits (3765), Expect = 0.0
Identity = 706/722 (97.78%), Postives = 706/722 (97.78%), Query Frame = 0

Query: 80  SRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQF 139
           SRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQF
Sbjct: 5   SRTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQF 64

Query: 140 SSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIE 199
           SSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIE
Sbjct: 65  SSRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIE 124

Query: 200 WFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDN 259
           WFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDN
Sbjct: 125 WFKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDN 184

Query: 260 GVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN 319
           GVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN
Sbjct: 185 GVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCN 244

Query: 320 AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVV 379
           AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVV
Sbjct: 245 AVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVV 304

Query: 380 PTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVY 439
           PTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVY
Sbjct: 305 PTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVY 364

Query: 440 WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLV 499
           WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLV
Sbjct: 365 WCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLV 424

Query: 500 RSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF 559
           RSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF
Sbjct: 425 RSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGF 484

Query: 560 MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ 619
           MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ
Sbjct: 485 MITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQ 544

Query: 620 ERYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDVINVSGH 679
           ERYEMTYFKPFPGYYFTGDGCTR                DKDGYYWLTGRVDDVINVSGH
Sbjct: 545 ERYEMTYFKPFPGYYFTGDGCTR----------------DKDGYYWLTGRVDDVINVSGH 604

Query: 680 RIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRK 739
           RIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRK
Sbjct: 605 RIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRK 664

Query: 740 QIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELA 799
           QIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELA
Sbjct: 665 QIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELA 710

Query: 800 DC 801
           DC
Sbjct: 725 DC 710

BLAST of MC08g1153 vs. ExPASy TrEMBL
Match: A0A6J1D3C3 (Acetate--CoA ligase OS=Momordica charantia OX=3673 GN=LOC111016935 PE=3 SV=1)

HSP 1 Score: 1453 bits (3762), Expect = 0.0
Identity = 705/721 (97.78%), Postives = 705/721 (97.78%), Query Frame = 0

Query: 81  RTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFS 140
           RTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFS
Sbjct: 5   RTSGEQAQVVMGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFS 64

Query: 141 SRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEW 200
           SRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEW
Sbjct: 65  SRAHVSSHQKYLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEW 124

Query: 201 FKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNG 260
           FKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNG
Sbjct: 125 FKGGITNICYNCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNG 184

Query: 261 VKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA 320
           VKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA
Sbjct: 185 VKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNA 244

Query: 321 VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVP 380
           VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVP
Sbjct: 245 VKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVP 304

Query: 381 TYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYW 440
           TYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYW
Sbjct: 305 TYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYW 364

Query: 441 CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVR 500
           CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVR
Sbjct: 365 CTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVR 424

Query: 501 SLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM 560
           SLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM
Sbjct: 425 SLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFM 484

Query: 561 ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE 620
           ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE
Sbjct: 485 ITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQE 544

Query: 621 RYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDVINVSGHR 680
           RYEMTYFKPFPGYYFTGDGCTR                DKDGYYWLTGRVDDVINVSGHR
Sbjct: 545 RYEMTYFKPFPGYYFTGDGCTR----------------DKDGYYWLTGRVDDVINVSGHR 604

Query: 681 IGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQ 740
           IGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQ
Sbjct: 605 IGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQ 664

Query: 741 IGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELAD 800
           IGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELAD
Sbjct: 665 IGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELAD 709

BLAST of MC08g1153 vs. ExPASy TrEMBL
Match: A0A6J1D2B5 (Acetate--CoA ligase OS=Momordica charantia OX=3673 GN=LOC111016935 PE=3 SV=1)

HSP 1 Score: 1435 bits (3715), Expect = 0.0
Identity = 695/711 (97.75%), Postives = 695/711 (97.75%), Query Frame = 0

Query: 91  MGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQK 150
           MGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQK
Sbjct: 1   MGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQK 60

Query: 151 YLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICY 210
           YLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICY
Sbjct: 61  YLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICY 120

Query: 211 NCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIY 270
           NCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIY
Sbjct: 121 NCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIY 180

Query: 271 MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL 330
           MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL
Sbjct: 181 MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL 240

Query: 331 KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEW 390
           KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEW
Sbjct: 241 KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEW 300

Query: 391 VDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITG 450
           VDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITG
Sbjct: 301 VDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITG 360

Query: 451 HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHV 510
           HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHV
Sbjct: 361 HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHV 420

Query: 511 TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ 570
           TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ
Sbjct: 421 TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ 480

Query: 571 KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPF 630
           KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPF
Sbjct: 481 KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPF 540

Query: 631 PGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 690
           PGYYFTGDGCTR                DKDGYYWLTGRVDDVINVSGHRIGTAEVESAL
Sbjct: 541 PGYYFTGDGCTR----------------DKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 600

Query: 691 VSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRI 750
           VSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRI
Sbjct: 601 VSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRI 660

Query: 751 HWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELADC 801
           HWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELADC
Sbjct: 661 HWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELADC 695

BLAST of MC08g1153 vs. ExPASy TrEMBL
Match: A0A0A0L7C4 (Acetate--CoA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G253500 PE=3 SV=1)

HSP 1 Score: 1373 bits (3553), Expect = 0.0
Identity = 688/824 (83.50%), Postives = 721/824 (87.50%), Query Frame = 0

Query: 1   VATSSC-FIRPFPA--NPTFLFPSASSANNSVHKIHNWKQPNYKKRQVNTFHSYYYFNSC 60
           + TSSC  +RPFPA  NP  LFPSA++A NS +   N    N K+R +NTFH++ YFNS 
Sbjct: 5   IVTSSCNAVRPFPASSNPGILFPSAAAAINSDYP--NPYFHNCKERHINTFHNHLYFNST 64

Query: 61  --------------LRPSCARSFGFTRSSSGFCCSFSRTSGEQAQ-------VVMGFTDL 120
                         LRP  A SF  + S S F  S +R  G            + G TD+
Sbjct: 65  SSSFSSSTTTSSCLLRPPFAGSF-LSSSLSTFSSSLTRIRGSDGDGRRDKNLSMGGLTDV 124

Query: 121 TAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQKYLEMYK 180
            A      ++SSAARKIPRLNA VLGES A+EDDQLI PSD FSS AH+SS+ +YLEMYK
Sbjct: 125 AA------LESSAARKIPRLNAAVLGESPATEDDQLIFPSDLFSSNAHISSYHQYLEMYK 184

Query: 181 RSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICYNCLDRN 240
           RSIEDPAGFWSDIASSEFFWK +WDQ VYSENLDVRKGEIKIEWFKGGITNICYNCLDRN
Sbjct: 185 RSIEDPAGFWSDIASSEFFWKQKWDQQVYSENLDVRKGEIKIEWFKGGITNICYNCLDRN 244

Query: 241 IEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIYMPMLME 300
           IEAGLG+K+ALYWEGNEPGFDGTLTY ELLHKVCQLAN+LKD GV+KGDAVVIYMPMLME
Sbjct: 245 IEAGLGEKIALYWEGNEPGFDGTLTYIELLHKVCQLANFLKDKGVRKGDAVVIYMPMLME 304

Query: 301 LPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDA 360
           LPIAMLACARIGA+HSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSK IHLKDIVDA
Sbjct: 305 LPIAMLACARIGAVHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKAIHLKDIVDA 364

Query: 361 ALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFP 420
           AL ESAQN VSVATCL+YENESAL REST WKEGRDIWWQDVVPTYPTTC VEWVDAEFP
Sbjct: 365 ALIESAQNGVSVATCLSYENESALNRESTKWKEGRDIWWQDVVPTYPTTCPVEWVDAEFP 424

Query: 421 LFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITGHSYVTY 480
           LFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKP DVYWCTADCGWITGHSYVTY
Sbjct: 425 LFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPSDVYWCTADCGWITGHSYVTY 484

Query: 481 GPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRK 540
           GPLLNGASVVV+EGAPNYPD GRCWDIVDK+KVTIFYTAPTLVRSLMREGNEHVTRYSRK
Sbjct: 485 GPLLNGASVVVFEGAPNYPDHGRCWDIVDKYKVTIFYTAPTLVRSLMREGNEHVTRYSRK 544

Query: 541 SLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSAT 600
           SLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSAT
Sbjct: 545 SLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSAT 604

Query: 601 FPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFT 660
           FPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWP+AFRTLYNDQERYE+TYFKPFPGYYFT
Sbjct: 605 FPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPSAFRTLYNDQERYEVTYFKPFPGYYFT 664

Query: 661 GDGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHC 720
           GDGCTR                DKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHP C
Sbjct: 665 GDGCTR----------------DKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQC 724

Query: 721 AEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGL 780
           AEAAVVG+EHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPD+IHWAPGL
Sbjct: 725 AEAAVVGVEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDKIHWAPGL 784

Query: 781 PKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELAD 800
           PKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVV+QLIELAD
Sbjct: 785 PKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVNQLIELAD 803

BLAST of MC08g1153 vs. TAIR 10
Match: AT5G36880.1 (acetyl-CoA synthetase )

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 562/710 (79.15%), Postives = 626/710 (88.17%), Query Frame = 0

Query: 91  MGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQK 150
           M    L   + ++Q+ S A  KI +LNA VLGESLASE++ L+ PS +FS +A VSS Q+
Sbjct: 1   MSSNSLRHVESMSQLPSGAG-KISQLNAVVLGESLASEENDLVFPSKEFSGQALVSSPQQ 60

Query: 151 YLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICY 210
           Y+EM+KRS++DPA FWSDIA SEF+WK +W   V+SENLDVRKG I IEWFKGGITNICY
Sbjct: 61  YMEMHKRSMDDPAAFWSDIA-SEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICY 120

Query: 211 NCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIY 270
           NCLD+N+EAGLGDK A++WEGNE G D +LTY+ELL +VCQLANYLKDNGVKKGDAVVIY
Sbjct: 121 NCLDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIY 180

Query: 271 MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL 330
           +PMLMELPIAMLACARIGA+HSVVFAGFS++SLAQRI+DCKP +++TCNAVKRG K I+L
Sbjct: 181 LPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINL 240

Query: 331 KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEW 390
           K IVDAAL +S+++ VSV  CLTY+N  A  RE+T W+ GRD+WWQDV+  YPT+C VEW
Sbjct: 241 KAIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEW 300

Query: 391 VDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITG 450
           VDAE PLFLLYTSGSTGKPKGVLHTTGGYM+YTATTFKYAFDYK  DVYWCTADCGWITG
Sbjct: 301 VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITG 360

Query: 451 HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHV 510
           HSYVTYGP+LNGA+VVV+EGAPNYPDPGRCWDIVDK+KV+IFYTAPTLVRSLMR+ ++ V
Sbjct: 361 HSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFV 420

Query: 511 TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ 570
           TR+SRKSLRVLGSVGEPINPSAWRWFFNVVG+SRCPISDTWWQTETGGFMITPLPGAWPQ
Sbjct: 421 TRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQ 480

Query: 571 KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPF 630
           KPGSATFPFFGVQPV+VDEKGNEIEGECSGYLC+K SWP AFRTL+ D ERYE TYFKPF
Sbjct: 481 KPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPF 540

Query: 631 PGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 690
            GYYF+GDGC                SRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL
Sbjct: 541 AGYYFSGDGC----------------SRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 600

Query: 691 VSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRI 750
           V HP CAEAAVVGIEHEVKGQGIYAFVTL++GV YSEELRKSL+L VR QIGAFAAPDRI
Sbjct: 601 VLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRI 660

Query: 751 HWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELAD 801
           HWAPGLPKTRSGKIMRRILRKIAS+QL+ELGDTSTLADP VVDQLI LAD
Sbjct: 661 HWAPGLPKTRSGKIMRRILRKIASRQLEELGDTSTLADPSVVDQLIALAD 692

BLAST of MC08g1153 vs. TAIR 10
Match: AT5G36880.2 (acetyl-CoA synthetase )

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 562/710 (79.15%), Postives = 626/710 (88.17%), Query Frame = 0

Query: 91  MGFTDLTAPDRLTQVDSSAARKIPRLNATVLGESLASEDDQLILPSDQFSSRAHVSSHQK 150
           M    L   + ++Q+ S A  KI +LNA VLGESLASE++ L+ PS +FS +A VSS Q+
Sbjct: 51  MSSNSLRHVESMSQLPSGAG-KISQLNAVVLGESLASEENDLVFPSKEFSGQALVSSPQQ 110

Query: 151 YLEMYKRSIEDPAGFWSDIASSEFFWKHRWDQLVYSENLDVRKGEIKIEWFKGGITNICY 210
           Y+EM+KRS++DPA FWSDIA SEF+WK +W   V+SENLDVRKG I IEWFKGGITNICY
Sbjct: 111 YMEMHKRSMDDPAAFWSDIA-SEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICY 170

Query: 211 NCLDRNIEAGLGDKVALYWEGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKKGDAVVIY 270
           NCLD+N+EAGLGDK A++WEGNE G D +LTY+ELL +VCQLANYLKDNGVKKGDAVVIY
Sbjct: 171 NCLDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIY 230

Query: 271 MPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKRGSKVIHL 330
           +PMLMELPIAMLACARIGA+HSVVFAGFS++SLAQRI+DCKP +++TCNAVKRG K I+L
Sbjct: 231 LPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINL 290

Query: 331 KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTYPTTCTVEW 390
           K IVDAAL +S+++ VSV  CLTY+N  A  RE+T W+ GRD+WWQDV+  YPT+C VEW
Sbjct: 291 KAIVDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEW 350

Query: 391 VDAEFPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDVYWCTADCGWITG 450
           VDAE PLFLLYTSGSTGKPKGVLHTTGGYM+YTATTFKYAFDYK  DVYWCTADCGWITG
Sbjct: 351 VDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADCGWITG 410

Query: 451 HSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHV 510
           HSYVTYGP+LNGA+VVV+EGAPNYPDPGRCWDIVDK+KV+IFYTAPTLVRSLMR+ ++ V
Sbjct: 411 HSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIVDKYKVSIFYTAPTLVRSLMRDDDKFV 470

Query: 511 TRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRCPISDTWWQTETGGFMITPLPGAWPQ 570
           TR+SRKSLRVLGSVGEPINPSAWRWFFNVVG+SRCPISDTWWQTETGGFMITPLPGAWPQ
Sbjct: 471 TRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQ 530

Query: 571 KPGSATFPFFGVQPVLVDEKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPF 630
           KPGSATFPFFGVQPV+VDEKGNEIEGECSGYLC+K SWP AFRTL+ D ERYE TYFKPF
Sbjct: 531 KPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDHERYETTYFKPF 590

Query: 631 PGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 690
            GYYF+GDGC                SRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL
Sbjct: 591 AGYYFSGDGC----------------SRDKDGYYWLTGRVDDVINVSGHRIGTAEVESAL 650

Query: 691 VSHPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRI 750
           V HP CAEAAVVGIEHEVKGQGIYAFVTL++GV YSEELRKSL+L VR QIGAFAAPDRI
Sbjct: 651 VLHPQCAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRI 710

Query: 751 HWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTLADPGVVDQLIELAD 801
           HWAPGLPKTRSGKIMRRILRKIAS+QL+ELGDTSTLADP VVDQLI LAD
Sbjct: 711 HWAPGLPKTRSGKIMRRILRKIASRQLEELGDTSTLADPSVVDQLIALAD 742

BLAST of MC08g1153 vs. TAIR 10
Match: AT5G23050.1 (acyl-activating enzyme 17 )

HSP 1 Score: 159.5 bits (402), Expect = 1.2e-38
Identity = 168/658 (25.53%), Postives = 276/658 (41.95%), Query Frame = 0

Query: 155 YKRSIEDPAGFWS-DIASSEFFWKHRWDQL--VYSE------NLDVRKGEIKIEWFKGGI 214
           YK  +   + F    +++ E +WK   D+L  ++S         D        +W  G  
Sbjct: 108 YKNPVSSFSSFQEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGDNPGGKWLPGAY 167

Query: 215 TNICYNCLDRNIEAGLGDKVALYW--EGNEPGFDGTLTYTELLHKVCQLANYLKDNGVKK 274
            N   NCL    +  L D + + W  EG++     T+T  EL  +V   A+ L   G+++
Sbjct: 168 LNPARNCLTNGFKRRL-DDIVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALGLEE 227

Query: 275 GDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGFSSESLAQRIIDCKPKIVITCNAVKR 334
             A+ + MPM +E  I  LA    G +   +   FS   ++ R+   K K + T + + R
Sbjct: 228 ESAIAVDMPMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDVIIR 287

Query: 335 GSKVIHL-KDIVDAALTESAQNEVSVATCLTYENESALKRESTTWKEGRDIWWQDVVPTY 394
           G K I L + +VDA        E  +A  +     S   +        +D+ W + +   
Sbjct: 288 GDKSIPLYRRVVDA--------EAPLAIVVPARGSSCRMK-----LREKDLSWNNFLGNA 347

Query: 395 PTTCTVEWVDAEFP----LFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPCDV 454
                VE+V AE P      +L++SG+TG+PK +  T    +   A  + +  D +  DV
Sbjct: 348 RNLRGVEYVAAEKPAGAYTNILFSSGTTGEPKAIPWTNISPLKSAADAWCH-LDVQRGDV 407

Query: 455 YWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDPGRCWDIVDKHKVTIFYTAPTL 514
                + GW+ G  ++ Y  L+NGA + +Y G+P  P   +    V   +V++    P++
Sbjct: 408 VAWPTNLGWMMG-PWLVYASLINGACMGLYNGSPLGPTFAK---FVQDAEVSVLGVIPSI 467

Query: 515 VRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFFNVVGESRC-PISDTWWQTETG 574
           VR+   + +   + Y    +R  GS GE  N   + W   ++G +   P+ +    TE G
Sbjct: 468 VRTW--QNSNSTSGYDWSRIRCFGSTGEASNIDEYLW---LMGRAHYKPVIEYCGGTEIG 527

Query: 575 GFMITPLPGAWPQKPGSATF--PFFGVQPVLVDEKGNEIEGECSGY--LCLKSSWPAAFR 634
           G  I+   G+  Q    A F     G +  ++DE  N I    +G   L L      A  
Sbjct: 528 GSFIS---GSLLQPQSLAAFSTAAMGCKLFILDEDSNPIPPYAAGVGELALCPHMFGASS 587

Query: 635 TLYNDQERYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTLSSRDKDGYYWLTGRVDDV 694
           TL N    +   YF+  P   F G          I+     L  R   GYY   GR DD 
Sbjct: 588 TLLNG--NHFKVYFQGMP--TFQG---------QILRRHGDLFERTSKGYYRAHGRADDT 647

Query: 695 INVSGHRIGTAEVESALVS-HPHCAEAAVVGIEHEVKGQGIYAFVTLVDGVSYSEE---- 754
           +N+ G ++G+ E+E    S      E A +G+     G        +     +S      
Sbjct: 648 MNLGGIKVGSIEIERVCNSVDDSVLETAAIGVPPPSGGPEQLVIAVVFKSPEFSNPDLNL 707

Query: 755 LRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQLDELGDTSTL 787
           L+KS    ++K++        +   P LP+T + K+MRR+LR    +QL + G  S L
Sbjct: 708 LKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTATNKVMRRVLR----QQLTQTGLNSKL 721

BLAST of MC08g1153 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 124.0 bits (310), Expect = 5.4e-28
Identity = 123/541 (22.74%), Postives = 233/541 (43.07%), Query Frame = 0

Query: 240 LTYTELLHKVCQLANYL-KDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGF 299
           LT+++L   V ++A+ L  + G+++GD V+I  P  + +P+  L+   +GA+ +      
Sbjct: 64  LTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLN 123

Query: 300 SSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENES 359
           +S  ++++I D  P +V T   +     V     + D  + +   + + V   L     S
Sbjct: 124 TSGEISKQIADSNPTLVFTTRQLAPKLPVAISVVLTDDEVYQELTSAIRVVGIL-----S 183

Query: 360 ALKRESTTWKEGRDIWWQDVVPTYPTTCTVEWVDAEFPLFLLYTSGSTGKPKGVLHTTGG 419
            + ++  + +  RD                  V+ +    +LY+SG+TG  KGV+     
Sbjct: 184 EMVKKEPSGQRVRD-----------------RVNQDDTAMMLYSSGTTGPSKGVI---SS 243

Query: 420 YMVYTATTFKYAFD-YKPCDVYWCTADCGWITGHSYVTYGPLLNGASVVVYEGAPNYPDP 479
           +   TA   ++  D  K  D++ CT       G      G +  G++VV+      +   
Sbjct: 244 HRNLTAHVARFISDNLKRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHD-- 303

Query: 480 GRCWDIVDKHKVTIFYTAPTLVRSLMREGNEHVTRYSRKSLRVLGSVGEPINPSAWRWFF 539
               D V+KH+ T    AP ++ +++ + +    +Y   SL+ +   G P++      F 
Sbjct: 304 --MMDAVEKHRATALALAPPVLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFL 363

Query: 540 NVVGESRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVLVDEKGNEIEG- 599
                    I   +  TE+ G           ++ G+A      V+  +VD       G 
Sbjct: 364 EKY--PTVDILQGYALTESNGGGAFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGI 423

Query: 600 ECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTRINLFSIITVLWTLS 659
             +G L LK   P+  +  + +QE    T      G+  TGD C                
Sbjct: 424 NQTGELWLKG--PSISKGYFKNQEATNETI--NLEGWLKTGDLC---------------- 483

Query: 660 SRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEVKGQGIYAF 719
             D+DG+ ++  R+ ++I   G+++  AE+E+ L++HP   +AAV+    +  GQ   A+
Sbjct: 484 YIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDKEAGQYPMAY 543

Query: 720 VTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRKIASKQ 778
           V      + SE   K +I  + KQ+  +    ++ +   +PKT SGK +R+ L K+A+ +
Sbjct: 544 VVRKHESNLSE---KQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLIKLATSK 550

BLAST of MC08g1153 vs. TAIR 10
Match: AT3G48990.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 122.9 bits (307), Expect = 1.2e-27
Identity = 140/541 (25.88%), Postives = 222/541 (41.04%), Query Frame = 0

Query: 240 LTYTELLHKVCQLANYL-KDNGVKKGDAVVIYMPMLMELPIAMLACARIGAIHSVVFAGF 299
           LT+  L   + + A+ L  D G+K GD V +  P  +E  I  LA  R  A  + + A +
Sbjct: 31  LTHARLHDLIERAASRLVSDAGIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPLNAAY 90

Query: 300 SSESLAQRIIDCKPKIVITCNAVKRGSKVIHLKDIVDAALTESAQNEVSVATCLTYENES 359
           ++E     + D   K+++T    K G+           A  + A +++ ++         
Sbjct: 91  TAEEFEFYLSDSDSKLLLTS---KEGN-----------APAQEAASKLKISHV------- 150

Query: 360 ALKRESTTWKEGRDIWWQDVVPTYPTTCTVEWVD-AEFPLFLLYTSGSTGKPKGVLHTTG 419
                +T    G D+               E V+  +     L+TSG+T +PKGV     
Sbjct: 151 ----TATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGV----- 210

Query: 420 GYMVYTATTFKYAFDYKPCD-VYWCT-ADCGWITGHSYVTYGPLLNGASVVVYEGAPNYP 479
                  T    A   K    VY  T +D   I    +  +G L    S +    A   P
Sbjct: 211 -----PLTQLNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLP 270

Query: 480 DPGR-----CWDIVDKHKVTIFYTAPTLVRSLM-REGNEHVTRYSRKSLRVLGSVGEPIN 539
             GR      W  + K+  T +   PT+ + ++ R  +   T Y +  LR + S    + 
Sbjct: 271 AAGRFSATTFWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPK--LRFIRSCSASLA 330

Query: 540 PSAWRWFFNVVGESRCPISDTWWQTETGGFMIT-PLPGAWPQKPGSATFPFFGVQPVLVD 599
           P          G    P+ + +  TE    M + PLP   P KPGS   P  G +  +++
Sbjct: 331 PVILSRLEEAFG---APVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKP-VGQEMAILN 390

Query: 600 EKGNEIEGECSGYLCLKSSWPAAFRTLYNDQERYEMTYFKPFPGYYFTGDGCTRINLFSI 659
           EKG   E    G +C++   P   +   N+ E  +  +   F G++ TGD    I  F  
Sbjct: 391 EKGEIQEPNNKGEVCIRG--PNVTKGYKNNPEANKAGF--EF-GWFHTGD----IGYF-- 450

Query: 660 ITVLWTLSSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPHCAEAAVVGIEHEV 719
                     D DGY  L GR+ ++IN  G +I   EV++ L++HP  ++    G+  E 
Sbjct: 451 ----------DTDGYLHLVGRIKELINRGGEKISPIEVDAVLLTHPDVSQGVAFGVPDEK 506

Query: 720 KGQGIYAFVTLVDGVSYSEELRKSLILTVRKQIGAFAAPDRIHWAPGLPKTRSGKIMRRI 770
            G+ I   V   +G + +EE  K+     +K + AF  P R+     LPKT SGKI RRI
Sbjct: 511 YGEEINCAVIPREGTTVTEEDIKAF---CKKNLAAFKVPKRVFITDNLPKTASGKIQRRI 506