Homology
BLAST of MC10g0175 vs. ExPASy Swiss-Prot
Match:
Q39111 (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)
HSP 1 Score: 428.7 bits (1101), Expect = 6.7e-119
Identity = 206/377 (54.64%), Postives = 264/377 (70.03%), Query Frame = 0
Query: 1 MAMECMTTPSPSLEEYKADNGALGGSTE--------FKAESVTPENFIWPDEFKAQDSLP 60
MA+ C TT SP+ +E++ T +S P FIWPDE K +P
Sbjct: 1 MAILC-TTTSPAEKEHEPKQDLEKDQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIP 60
Query: 61 ELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFF 120
EL+VP ID+ + EA R++ EAC KHGFF+VVNHGV ++ D H+ M+ FF
Sbjct: 61 ELNVPFIDL-----SSQDSTLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFF 120
Query: 121 ELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTL 180
++PL KQKA RK G+S GY SS+ GRFS KLPWKET S + + S V DY DTL
Sbjct: 121 DMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTL 180
Query: 181 GQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCE 240
GQEF GKVYQ+ C+AM+ LSL+++ELLG+SLG+ R+ F+ +E++DSIMRLN+YP C+
Sbjct: 181 GQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQ 240
Query: 241 NPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNG 300
P+LTLGTGPHCDP+S+TILHQDHV+GLQV VD+QW SI P P +FVVNIGDTFMAL+NG
Sbjct: 241 TPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNG 300
Query: 301 VYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQ 360
++KSC HRAVVN + RKS+AFFLCP +K+V+ P ++EK RK+PDFTW M E TQ
Sbjct: 301 IFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQ 360
Query: 361 KFYRADSNTLKAFTTWL 370
K YRAD NTL +F+ W+
Sbjct: 361 KHYRADVNTLDSFSNWV 371
BLAST of MC10g0175 vs. ExPASy Swiss-Prot
Match:
Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)
HSP 1 Score: 412.5 bits (1059), Expect = 5.0e-114
Identity = 206/378 (54.50%), Postives = 251/378 (66.40%), Query Frame = 0
Query: 1 MAMECMTTPSPSLEEYKA--DNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPH 60
MA EC+ T E K D+ + P+ F+WPD K + L VP
Sbjct: 1 MATECIATVPQIFSENKTKEDSSIFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPL 60
Query: 61 IDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQ 120
ID+ LSGD A EA RLV +A KHGFF++ NHGVD ++ + MD FF+ P +
Sbjct: 61 IDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACE 120
Query: 121 KQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL-SHKDHSSLVHDYVRDTLGQEFS 180
KQKA RK G+S GY SS++GRFS KLPWKET S + K HS V D+V +G +
Sbjct: 121 KQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYE 180
Query: 181 HHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELT 240
GKVYQE +AM LSL+++ELLGMSLG+ R FK+ +ED DSI RLNYYP C+ PEL
Sbjct: 181 DFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELA 240
Query: 241 LGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSC 300
LGTGPHCDPTS+TILHQD V GLQV VD++W SIPP P +FVVNIGDTFMALTNG YKSC
Sbjct: 241 LGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSC 300
Query: 301 FHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVE--KNPPRKFPDFTWPMLHELTQKFY 360
HRAVVN + RK+ AFFLCP EK+V+ PE+LV K+ RK+PDFTW M E TQK Y
Sbjct: 301 LHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHY 360
Query: 361 RADSNTLKAFTTWLQQQK 374
RAD NTL F+ WL+ ++
Sbjct: 361 RADMNTLDEFSIWLKNRR 378
BLAST of MC10g0175 vs. ExPASy Swiss-Prot
Match:
Q39110 (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)
HSP 1 Score: 411.8 bits (1057), Expect = 8.5e-114
Identity = 192/334 (57.49%), Postives = 244/334 (73.05%), Query Frame = 0
Query: 36 PENFIWPDEFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHG 95
P FIWPD+ K ++ EL VP ID++ LLS D +A RL+ EAC+KHGFF+VVNHG
Sbjct: 41 PNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLVVNHG 100
Query: 96 VDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL 155
+ ++ D H+ FF++PL +KQ+ RK G+S GY SS+ GRFS KLPWKET S +
Sbjct: 101 ISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFC 160
Query: 156 SHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKL 215
S V DY D LG F GKVYQE C+AM+ LSL+++ELLG+SLG+ R+ F++
Sbjct: 161 DDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREF 220
Query: 216 YEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTP 275
+E++DSIMRLNYYP C P+LTLGTGPHCDPTS+TILHQDHV+GLQV V++QW SI P P
Sbjct: 221 FEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNP 280
Query: 276 DSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNP 335
+FVVNIGDTFMAL+N YKSC HRAVVN + RKS+AFFLCP +++V P +L++
Sbjct: 281 KAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLDSIT 340
Query: 336 PRKFPDFTWPMLHELTQKFYRADSNTLKAFTTWL 370
R++PDFTW M E TQK YRAD NTL+AF+ WL
Sbjct: 341 SRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373
BLAST of MC10g0175 vs. ExPASy Swiss-Prot
Match:
O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)
HSP 1 Score: 403.7 bits (1036), Expect = 2.3e-111
Identity = 191/339 (56.34%), Postives = 244/339 (71.98%), Query Frame = 0
Query: 36 PENFIWPD-EFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNH 95
P FIWP+ E D+ ELHVP I+I +LSGD A E RLV EAC +HGFF VVNH
Sbjct: 18 PSQFIWPEGESPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGEACERHGFFQVVNH 77
Query: 96 GVDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCY 155
G+D ++ D H+C+D FF +PL +KQ+A R+ G+S GY SS+ GRF+ KLPWKET S +
Sbjct: 78 GIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLS--F 137
Query: 156 LSHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKK 215
S +LV DY+ TLG++ G+VY C M+ LSLE++E+LG SLG+GR +++
Sbjct: 138 RSCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRR 197
Query: 216 LYEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPT 275
+E +DSIMRLNYYP C+ P TLGTGPHCDPTS+TILHQD+V GLQVH + +W SI P
Sbjct: 198 FFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPR 257
Query: 276 PDSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKN 335
D+FVVNIGDTFMAL+NG YKSC HRAVVN + RKS+AFFLCP +K+V P LV+ +
Sbjct: 258 ADAFVVNIGDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPGTLVDAS 317
Query: 336 PPRKFPDFTWPMLHELTQKFYRADSNTLKAFTTWLQQQK 374
PR +PDFTW L + TQK YRAD TL+ F++W+ QQ+
Sbjct: 318 NPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQQQ 354
BLAST of MC10g0175 vs. ExPASy Swiss-Prot
Match:
O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)
HSP 1 Score: 403.3 bits (1035), Expect = 3.0e-111
Identity = 192/339 (56.64%), Postives = 242/339 (71.39%), Query Frame = 0
Query: 36 PENFIWPD-EFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNH 95
P FIWP+ E D+ ELHVP IDI +LSGD E RLV EAC +HGFF VVNH
Sbjct: 18 PSQFIWPEGESPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACERHGFFQVVNH 77
Query: 96 GVDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCY 155
G+D ++ D H+C+D FF +PL +KQ+A R+ G+S GY SS+ GRF+ KLPWKET S +
Sbjct: 78 GIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKLPWKETLS--F 137
Query: 156 LSHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKK 215
S +LV DY+ TLG++ G+VY C M+ LSLE++E+LG SLG+GR +++
Sbjct: 138 RSCPSDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGESLGVGRAHYRR 197
Query: 216 LYEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPT 275
+E +DSIMRLNYYP C+ P TLGTGPHCDPTS+TILHQD+V GLQVH + +W SI P
Sbjct: 198 FFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPR 257
Query: 276 PDSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKN 335
D+FVVNIGDTFMAL+NG YKSC HRAVVN K RKS+AFFLCP +K+V P LV+
Sbjct: 258 ADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPGTLVDAA 317
Query: 336 PPRKFPDFTWPMLHELTQKFYRADSNTLKAFTTWLQQQK 374
PR +PDFTW L + TQK YRAD TL+ F++W+ QQ+
Sbjct: 318 NPRAYPDFTWRSLLDFTQKHYRADMKTLEVFSSWIVQQQ 354
BLAST of MC10g0175 vs. NCBI nr
Match:
XP_022141206.1 (gibberellin 20 oxidase 2-like [Momordica charantia])
HSP 1 Score: 786 bits (2030), Expect = 2.28e-287
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 0
Query: 1 MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID
Sbjct: 1 MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
Query: 61 IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ
Sbjct: 61 IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG
Sbjct: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT
Sbjct: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN 360
AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN
Sbjct: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN 360
Query: 361 TLKAFTTWLQQQK 373
TLKAFTTWLQQQK
Sbjct: 361 TLKAFTTWLQQQK 373
BLAST of MC10g0175 vs. NCBI nr
Match:
XP_022141149.1 (gibberellin 20 oxidase 2-like [Momordica charantia])
HSP 1 Score: 680 bits (1755), Expect = 1.74e-245
Identity = 322/373 (86.33%), Postives = 343/373 (91.96%), Query Frame = 0
Query: 1 MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
MA+ECMT+ SP E KADN A GGS EFKAE+V PE FIWPDE K +DS+PELHVPHID
Sbjct: 1 MAIECMTSSSPV--ESKADNAAFGGSVEFKAETVVPEKFIWPDEMKPEDSVPELHVPHID 60
Query: 61 IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
+KK LSG+EG EEAMRLVDEACRKHGFFVVVNHGVDMGIM+DLHKCMDEFFELPLD KQ
Sbjct: 61 MKKFLSGEEGAVEEAMRLVDEACRKHGFFVVVNHGVDMGIMRDLHKCMDEFFELPLDHKQ 120
Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
KA RK GDSYGY SS++GRFS KLPWKETFS+ YLS++DHSS VHDYV DTLGQEFSHHG
Sbjct: 121 KAQRKFGDSYGYASSFLGRFSTKLPWKETFSLRYLSNEDHSSAVHDYVCDTLGQEFSHHG 180
Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
KVYQEC KAMAELSLE+VELLGMSLGLGREEFKK+YED+DSI+RLN+YP CENPELTLGT
Sbjct: 181 KVYQECSKAMAELSLEIVELLGMSLGLGREEFKKVYEDNDSILRLNFYPRCENPELTLGT 240
Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNG+YKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGLYKSCFHR 300
Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN 360
AVVNCKE RKSVAFFLCP AEKLVRAPE+LV KNPPRKFPDFTWPML ELTQKFYRADSN
Sbjct: 301 AVVNCKEARKSVAFFLCPAAEKLVRAPEELVVKNPPRKFPDFTWPMLLELTQKFYRADSN 360
Query: 361 TLKAFTTWLQQQK 373
TL+AFT WLQQQK
Sbjct: 361 TLEAFTAWLQQQK 371
BLAST of MC10g0175 vs. NCBI nr
Match:
CAA70329.1 (gibberellin 20-oxidase [Marah macrocarpa])
HSP 1 Score: 574 bits (1480), Expect = 1.19e-203
Identity = 272/374 (72.73%), Postives = 313/374 (83.69%), Query Frame = 0
Query: 1 MAMECM--TTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPH 60
MA+ CM T SP+ E + A GG+ ES PENFIWPDEFKA PELHVPH
Sbjct: 1 MAITCMMATESSPAEEVKREHQVAFGGA----GESSVPENFIWPDEFKATADAPELHVPH 60
Query: 61 IDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQ 120
ID+KK+LSGDE EEA RLVDEACRKHGFFVVVNHGVD +M +H+CM+EFF LPLD
Sbjct: 61 IDLKKVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFTLPLDV 120
Query: 121 KQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSH 180
KQKAHRK+G+++GY +S+IGRFS KLPWKETFS+ YL+H++ SS DYV LG EFSH
Sbjct: 121 KQKAHRKVGENFGYANSFIGRFSTKLPWKETFSLRYLAHEN-SSTARDYVSQVLGPEFSH 180
Query: 181 HGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTL 240
HG+VYQEC KA+++LSL +VELLG+SLG+ RE F+K YED+DSIMR+NYYP CE PELTL
Sbjct: 181 HGEVYQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEKPELTL 240
Query: 241 GTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCF 300
GTGPHCDPTSITILHQD VSGLQV+VDDQW+SIPPT DSFVVN+GDTFM+LTNGVYKSCF
Sbjct: 241 GTGPHCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGVYKSCF 300
Query: 301 HRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRAD 360
HRAVVNCKE RKS+AFFLCP EK+VRAPE+LVEK PPRKFPD+TWPML E+TQK+YRAD
Sbjct: 301 HRAVVNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQKYYRAD 360
Query: 361 SNTLKAFTTWLQQQ 372
SNT AFTTW+QQQ
Sbjct: 361 SNTFNAFTTWIQQQ 369
BLAST of MC10g0175 vs. NCBI nr
Match:
XP_022922616.1 (gibberellin 20 oxidase 2-like [Cucurbita moschata] >KAG6576758.1 Gibberellin 20 oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 560 bits (1444), Expect = 4.28e-198
Identity = 261/378 (69.05%), Postives = 317/378 (83.86%), Query Frame = 0
Query: 1 MAMECMTTP-----SPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELH 60
MA++CMTT S +E K + GS++ ES+ PENFIWP+EFKA++S+PELH
Sbjct: 1 MAIKCMTTAKSQGESKLKQEMKRKHRVEFGSSD---ESMVPENFIWPEEFKARESVPELH 60
Query: 61 VPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELP 120
VPHID++K +S +E EE RLVDEACRKHGFFV+VNHGVDM +MK++H+CMDEFF LP
Sbjct: 61 VPHIDLQKFMSENENDIEEVRRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLP 120
Query: 121 LDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQE 180
LD KQKA RK+G++YGY +S+IGRFS KLPWKETFS+ Y +H + HDYV LGQE
Sbjct: 121 LDVKQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHHP---ITHDYVCHHLGQE 180
Query: 181 FSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPE 240
FS GKVY+EC KA+++LSL++VELLG+SLG+ +++FKK +ED+DSI+RLNYYP C PE
Sbjct: 181 FSQQGKVYEECGKALSDLSLKIVELLGLSLGVPKQKFKKFFEDNDSIIRLNYYPPCNKPE 240
Query: 241 LTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYK 300
LTLGTGPHCDPTSITILHQDHVSGLQV+VDDQW+SIPP DSFV+NIGDTFMALTNGVYK
Sbjct: 241 LTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYK 300
Query: 301 SCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFY 360
SCFHRAVVNC+E RKS+ FFLCP +K+VRAP+++V+KNPPRKFPD+TWPML ELTQKFY
Sbjct: 301 SCFHRAVVNCREARKSIVFFLCPAGDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFY 360
Query: 361 RADSNTLKAFTTWLQQQK 373
R+DSNT KAFTTWLQ +K
Sbjct: 361 RSDSNTFKAFTTWLQHKK 372
BLAST of MC10g0175 vs. NCBI nr
Match:
AAD42693.1 (gibberellin 20-oxidase [Citrullus lanatus subsp. vulgaris])
HSP 1 Score: 557 bits (1436), Expect = 5.90e-197
Identity = 260/373 (69.71%), Postives = 314/373 (84.18%), Query Frame = 0
Query: 1 MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
MAM+CM + S E + G +E ES+ PENF+WPDEFKA+D PELHVPHID
Sbjct: 1 MAMKCMMSTGESKEMKSKLHRVEFGCSE---ESMVPENFVWPDEFKAKDGAPELHVPHID 60
Query: 61 IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
+ K L+G+E EEA RLVDEACRKHGFFV+VNHGVDM ++K +H+CMDEFF LP D KQ
Sbjct: 61 LHKFLNGNESDIEEATRLVDEACRKHGFFVLVNHGVDMELIKGVHECMDEFFTLPFDVKQ 120
Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
K+ RK G++YGY +S+IGRFS+ LPWKET S+ Y+++ + +S V D+V +G E SH G
Sbjct: 121 KSQRKFGENYGYANSFIGRFSNNLPWKETLSLPYVANHNSTS-VQDFVSKCVGPELSHQG 180
Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
KVYQEC KA+++LSL++VELLG+SLG+ +E+FKK YED+DSI+RLNYYP CE PELTLGT
Sbjct: 181 KVYQECGKALSDLSLKIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGT 240
Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
GPHCDPTSITILHQDHVSGLQV+VDD+W+SIPPT DSFV+NIGDTFMALTNGVYKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHR 300
Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN 360
AVVNCKEVRKS+AFFLCP A+K+VRAP+++VEKNPPRKFPD+TWPML ELTQKFYRADSN
Sbjct: 301 AVVNCKEVRKSMAFFLCPAADKVVRAPDEVVEKNPPRKFPDYTWPMLLELTQKFYRADSN 360
Query: 361 TLKAFTTWLQQQK 373
T +AF WL++QK
Sbjct: 361 TFQAFIPWLEKQK 369
BLAST of MC10g0175 vs. ExPASy TrEMBL
Match:
A0A6J1CIE9 (gibberellin 20 oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111011662 PE=3 SV=1)
HSP 1 Score: 786 bits (2030), Expect = 1.10e-287
Identity = 373/373 (100.00%), Postives = 373/373 (100.00%), Query Frame = 0
Query: 1 MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID
Sbjct: 1 MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
Query: 61 IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ
Sbjct: 61 IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG
Sbjct: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT
Sbjct: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN 360
AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN
Sbjct: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN 360
Query: 361 TLKAFTTWLQQQK 373
TLKAFTTWLQQQK
Sbjct: 361 TLKAFTTWLQQQK 373
BLAST of MC10g0175 vs. ExPASy TrEMBL
Match:
A0A6J1CHR5 (gibberellin 20 oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111011612 PE=3 SV=1)
HSP 1 Score: 680 bits (1755), Expect = 8.42e-246
Identity = 322/373 (86.33%), Postives = 343/373 (91.96%), Query Frame = 0
Query: 1 MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
MA+ECMT+ SP E KADN A GGS EFKAE+V PE FIWPDE K +DS+PELHVPHID
Sbjct: 1 MAIECMTSSSPV--ESKADNAAFGGSVEFKAETVVPEKFIWPDEMKPEDSVPELHVPHID 60
Query: 61 IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
+KK LSG+EG EEAMRLVDEACRKHGFFVVVNHGVDMGIM+DLHKCMDEFFELPLD KQ
Sbjct: 61 MKKFLSGEEGAVEEAMRLVDEACRKHGFFVVVNHGVDMGIMRDLHKCMDEFFELPLDHKQ 120
Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
KA RK GDSYGY SS++GRFS KLPWKETFS+ YLS++DHSS VHDYV DTLGQEFSHHG
Sbjct: 121 KAQRKFGDSYGYASSFLGRFSTKLPWKETFSLRYLSNEDHSSAVHDYVCDTLGQEFSHHG 180
Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
KVYQEC KAMAELSLE+VELLGMSLGLGREEFKK+YED+DSI+RLN+YP CENPELTLGT
Sbjct: 181 KVYQECSKAMAELSLEIVELLGMSLGLGREEFKKVYEDNDSILRLNFYPRCENPELTLGT 240
Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNG+YKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGLYKSCFHR 300
Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN 360
AVVNCKE RKSVAFFLCP AEKLVRAPE+LV KNPPRKFPDFTWPML ELTQKFYRADSN
Sbjct: 301 AVVNCKEARKSVAFFLCPAAEKLVRAPEELVVKNPPRKFPDFTWPMLLELTQKFYRADSN 360
Query: 361 TLKAFTTWLQQQK 373
TL+AFT WLQQQK
Sbjct: 361 TLEAFTAWLQQQK 371
BLAST of MC10g0175 vs. ExPASy TrEMBL
Match:
Q96498 (Gibberellin 20-oxidase OS=Marah macrocarpa OX=48242 PE=2 SV=1)
HSP 1 Score: 574 bits (1480), Expect = 5.74e-204
Identity = 272/374 (72.73%), Postives = 313/374 (83.69%), Query Frame = 0
Query: 1 MAMECM--TTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPH 60
MA+ CM T SP+ E + A GG+ ES PENFIWPDEFKA PELHVPH
Sbjct: 1 MAITCMMATESSPAEEVKREHQVAFGGA----GESSVPENFIWPDEFKATADAPELHVPH 60
Query: 61 IDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQ 120
ID+KK+LSGDE EEA RLVDEACRKHGFFVVVNHGVD +M +H+CM+EFF LPLD
Sbjct: 61 IDLKKVLSGDEKDVEEATRLVDEACRKHGFFVVVNHGVDKELMNKVHECMNEFFTLPLDV 120
Query: 121 KQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSH 180
KQKAHRK+G+++GY +S+IGRFS KLPWKETFS+ YL+H++ SS DYV LG EFSH
Sbjct: 121 KQKAHRKVGENFGYANSFIGRFSTKLPWKETFSLRYLAHEN-SSTARDYVSQVLGPEFSH 180
Query: 181 HGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTL 240
HG+VYQEC KA+++LSL +VELLG+SLG+ RE F+K YED+DSIMR+NYYP CE PELTL
Sbjct: 181 HGEVYQECGKALSDLSLRIVELLGLSLGISRETFRKFYEDNDSIMRMNYYPRCEKPELTL 240
Query: 241 GTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCF 300
GTGPHCDPTSITILHQD VSGLQV+VDDQW+SIPPT DSFVVN+GDTFM+LTNGVYKSCF
Sbjct: 241 GTGPHCDPTSITILHQDDVSGLQVYVDDQWHSIPPTEDSFVVNVGDTFMSLTNGVYKSCF 300
Query: 301 HRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRAD 360
HRAVVNCKE RKS+AFFLCP EK+VRAPE+LVEK PPRKFPD+TWPML E+TQK+YRAD
Sbjct: 301 HRAVVNCKEARKSMAFFLCPAVEKVVRAPEELVEKYPPRKFPDYTWPMLLEMTQKYYRAD 360
Query: 361 SNTLKAFTTWLQQQ 372
SNT AFTTW+QQQ
Sbjct: 361 SNTFNAFTTWIQQQ 369
BLAST of MC10g0175 vs. ExPASy TrEMBL
Match:
A0A6J1E4L1 (gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430571 PE=3 SV=1)
HSP 1 Score: 560 bits (1444), Expect = 2.07e-198
Identity = 261/378 (69.05%), Postives = 317/378 (83.86%), Query Frame = 0
Query: 1 MAMECMTTP-----SPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELH 60
MA++CMTT S +E K + GS++ ES+ PENFIWP+EFKA++S+PELH
Sbjct: 1 MAIKCMTTAKSQGESKLKQEMKRKHRVEFGSSD---ESMVPENFIWPEEFKARESVPELH 60
Query: 61 VPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELP 120
VPHID++K +S +E EE RLVDEACRKHGFFV+VNHGVDM +MK++H+CMDEFF LP
Sbjct: 61 VPHIDLQKFMSENENDIEEVRRLVDEACRKHGFFVLVNHGVDMELMKNVHECMDEFFSLP 120
Query: 121 LDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQE 180
LD KQKA RK+G++YGY +S+IGRFS KLPWKETFS+ Y +H + HDYV LGQE
Sbjct: 121 LDVKQKAQRKVGENYGYANSFIGRFSSKLPWKETFSLRYAAHHP---ITHDYVCHHLGQE 180
Query: 181 FSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPE 240
FS GKVY+EC KA+++LSL++VELLG+SLG+ +++FKK +ED+DSI+RLNYYP C PE
Sbjct: 181 FSQQGKVYEECGKALSDLSLKIVELLGLSLGVPKQKFKKFFEDNDSIIRLNYYPPCNKPE 240
Query: 241 LTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYK 300
LTLGTGPHCDPTSITILHQDHVSGLQV+VDDQW+SIPP DSFV+NIGDTFMALTNGVYK
Sbjct: 241 LTLGTGPHCDPTSITILHQDHVSGLQVYVDDQWHSIPPIKDSFVINIGDTFMALTNGVYK 300
Query: 301 SCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFY 360
SCFHRAVVNC+E RKS+ FFLCP +K+VRAP+++V+KNPPRKFPD+TWPML ELTQKFY
Sbjct: 301 SCFHRAVVNCREARKSIVFFLCPAGDKVVRAPDEIVDKNPPRKFPDYTWPMLLELTQKFY 360
Query: 361 RADSNTLKAFTTWLQQQK 373
R+DSNT KAFTTWLQ +K
Sbjct: 361 RSDSNTFKAFTTWLQHKK 372
BLAST of MC10g0175 vs. ExPASy TrEMBL
Match:
Q9XHR0 (Gibberellin 20-oxidase OS=Citrullus lanatus OX=3654 GN=WGA20OXI PE=2 SV=1)
HSP 1 Score: 557 bits (1436), Expect = 2.85e-197
Identity = 260/373 (69.71%), Postives = 314/373 (84.18%), Query Frame = 0
Query: 1 MAMECMTTPSPSLEEYKADNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPHID 60
MAM+CM + S E + G +E ES+ PENF+WPDEFKA+D PELHVPHID
Sbjct: 1 MAMKCMMSTGESKEMKSKLHRVEFGCSE---ESMVPENFVWPDEFKAKDGAPELHVPHID 60
Query: 61 IKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQKQ 120
+ K L+G+E EEA RLVDEACRKHGFFV+VNHGVDM ++K +H+CMDEFF LP D KQ
Sbjct: 61 LHKFLNGNESDIEEATRLVDEACRKHGFFVLVNHGVDMELIKGVHECMDEFFTLPFDVKQ 120
Query: 121 KAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTLGQEFSHHG 180
K+ RK G++YGY +S+IGRFS+ LPWKET S+ Y+++ + +S V D+V +G E SH G
Sbjct: 121 KSQRKFGENYGYANSFIGRFSNNLPWKETLSLPYVANHNSTS-VQDFVSKCVGPELSHQG 180
Query: 181 KVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELTLGT 240
KVYQEC KA+++LSL++VELLG+SLG+ +E+FKK YED+DSI+RLNYYP CE PELTLGT
Sbjct: 181 KVYQECGKALSDLSLKIVELLGLSLGVPKEKFKKFYEDNDSIIRLNYYPPCEKPELTLGT 240
Query: 241 GPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSCFHR 300
GPHCDPTSITILHQDHVSGLQV+VDD+W+SIPPT DSFV+NIGDTFMALTNGVYKSCFHR
Sbjct: 241 GPHCDPTSITILHQDHVSGLQVYVDDEWHSIPPTKDSFVINIGDTFMALTNGVYKSCFHR 300
Query: 301 AVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQKFYRADSN 360
AVVNCKEVRKS+AFFLCP A+K+VRAP+++VEKNPPRKFPD+TWPML ELTQKFYRADSN
Sbjct: 301 AVVNCKEVRKSMAFFLCPAADKVVRAPDEVVEKNPPRKFPDYTWPMLLELTQKFYRADSN 360
Query: 361 TLKAFTTWLQQQK 373
T +AF WL++QK
Sbjct: 361 TFQAFIPWLEKQK 369
BLAST of MC10g0175 vs. TAIR 10
Match:
AT5G51810.1 (gibberellin 20 oxidase 2 )
HSP 1 Score: 428.7 bits (1101), Expect = 4.8e-120
Identity = 206/377 (54.64%), Postives = 264/377 (70.03%), Query Frame = 0
Query: 1 MAMECMTTPSPSLEEYKADNGALGGSTE--------FKAESVTPENFIWPDEFKAQDSLP 60
MA+ C TT SP+ +E++ T +S P FIWPDE K +P
Sbjct: 1 MAILC-TTTSPAEKEHEPKQDLEKDQTSPLIFNPSLLNLQSQIPNQFIWPDEEKPSIDIP 60
Query: 61 ELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFF 120
EL+VP ID+ + EA R++ EAC KHGFF+VVNHGV ++ D H+ M+ FF
Sbjct: 61 ELNVPFIDL-----SSQDSTLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFF 120
Query: 121 ELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYLSHKDHSSLVHDYVRDTL 180
++PL KQKA RK G+S GY SS+ GRFS KLPWKET S + + S V DY DTL
Sbjct: 121 DMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTL 180
Query: 181 GQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCE 240
GQEF GKVYQ+ C+AM+ LSL+++ELLG+SLG+ R+ F+ +E++DSIMRLN+YP C+
Sbjct: 181 GQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQ 240
Query: 241 NPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNG 300
P+LTLGTGPHCDP+S+TILHQDHV+GLQV VD+QW SI P P +FVVNIGDTFMAL+NG
Sbjct: 241 TPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFMALSNG 300
Query: 301 VYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNPPRKFPDFTWPMLHELTQ 360
++KSC HRAVVN + RKS+AFFLCP +K+V+ P ++EK RK+PDFTW M E TQ
Sbjct: 301 IFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDILEKMKTRKYPDFTWSMFLEFTQ 360
Query: 361 KFYRADSNTLKAFTTWL 370
K YRAD NTL +F+ W+
Sbjct: 361 KHYRADVNTLDSFSNWV 371
BLAST of MC10g0175 vs. TAIR 10
Match:
AT5G07200.1 (gibberellin 20-oxidase 3 )
HSP 1 Score: 412.5 bits (1059), Expect = 3.5e-115
Identity = 206/378 (54.50%), Postives = 251/378 (66.40%), Query Frame = 0
Query: 1 MAMECMTTPSPSLEEYKA--DNGALGGSTEFKAESVTPENFIWPDEFKAQDSLPELHVPH 60
MA EC+ T E K D+ + P+ F+WPD K + L VP
Sbjct: 1 MATECIATVPQIFSENKTKEDSSIFDAKLLNQHSHHIPQQFVWPDHEKPSTDVQPLQVPL 60
Query: 61 IDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHGVDMGIMKDLHKCMDEFFELPLDQ 120
ID+ LSGD A EA RLV +A KHGFF++ NHGVD ++ + MD FF+ P +
Sbjct: 61 IDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDSFFKAPACE 120
Query: 121 KQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL-SHKDHSSLVHDYVRDTLGQEFS 180
KQKA RK G+S GY SS++GRFS KLPWKET S + K HS V D+V +G +
Sbjct: 121 KQKAQRKWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIHSQTVKDFVSKKMGDGYE 180
Query: 181 HHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKLYEDHDSIMRLNYYPTCENPELT 240
GKVYQE +AM LSL+++ELLGMSLG+ R FK+ +ED DSI RLNYYP C+ PEL
Sbjct: 181 DFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSDSIFRLNYYPQCKQPELA 240
Query: 241 LGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTPDSFVVNIGDTFMALTNGVYKSC 300
LGTGPHCDPTS+TILHQD V GLQV VD++W SIPP P +FVVNIGDTFMALTNG YKSC
Sbjct: 241 LGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVVNIGDTFMALTNGRYKSC 300
Query: 301 FHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVE--KNPPRKFPDFTWPMLHELTQKFY 360
HRAVVN + RK+ AFFLCP EK+V+ PE+LV K+ RK+PDFTW M E TQK Y
Sbjct: 301 LHRAVVNSERERKTFAFFLCPKGEKVVKPPEELVNGVKSGERKYPDFTWSMFLEFTQKHY 360
Query: 361 RADSNTLKAFTTWLQQQK 374
RAD NTL F+ WL+ ++
Sbjct: 361 RADMNTLDEFSIWLKNRR 378
BLAST of MC10g0175 vs. TAIR 10
Match:
AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )
HSP 1 Score: 411.8 bits (1057), Expect = 6.0e-115
Identity = 192/334 (57.49%), Postives = 244/334 (73.05%), Query Frame = 0
Query: 36 PENFIWPDEFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHG 95
P FIWPD+ K ++ EL VP ID++ LLS D +A RL+ EAC+KHGFF+VVNHG
Sbjct: 41 PNQFIWPDDEKPSINVLELDVPLIDLQNLLS-DPSSTLDASRLISEACKKHGFFLVVNHG 100
Query: 96 VDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL 155
+ ++ D H+ FF++PL +KQ+ RK G+S GY SS+ GRFS KLPWKET S +
Sbjct: 101 ISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFSTKLPWKETLSFRFC 160
Query: 156 SHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEFKKL 215
S V DY D LG F GKVYQE C+AM+ LSL+++ELLG+SLG+ R+ F++
Sbjct: 161 DDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLGLSLGVKRDYFREF 220
Query: 216 YEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPTP 275
+E++DSIMRLNYYP C P+LTLGTGPHCDPTS+TILHQDHV+GLQV V++QW SI P P
Sbjct: 221 FEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQVFVENQWRSIRPNP 280
Query: 276 DSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVEKNP 335
+FVVNIGDTFMAL+N YKSC HRAVVN + RKS+AFFLCP +++V P +L++
Sbjct: 281 KAFVVNIGDTFMALSNDRYKSCLHRAVVNSESERKSLAFFLCPKKDRVVTPPRELLDSIT 340
Query: 336 PRKFPDFTWPMLHELTQKFYRADSNTLKAFTTWL 370
R++PDFTW M E TQK YRAD NTL+AF+ WL
Sbjct: 341 SRRYPDFTWSMFLEFTQKHYRADMNTLQAFSDWL 373
BLAST of MC10g0175 vs. TAIR 10
Match:
AT1G60980.1 (gibberellin 20-oxidase 4 )
HSP 1 Score: 393.7 bits (1010), Expect = 1.7e-109
Identity = 185/340 (54.41%), Postives = 238/340 (70.00%), Query Frame = 0
Query: 36 PENFIWPDEFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHG 95
P+ F+WPD K ++P L VP ID+ LS D EA RLV EA +KHGFF+V NHG
Sbjct: 37 PQEFVWPDHEKPSKNVPILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFLVTNHG 96
Query: 96 VDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL 155
VD ++ HK MD FF+ P +K KA RK+G++ GY SS++GRF + LPWKET S +
Sbjct: 97 VDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETLSFSFS 156
Query: 156 ---SHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGLGREEF 215
+++S V +Y+ T+G + G VYQE + M+ LSL+++ELLGMSLG+ RE F
Sbjct: 157 PTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGIKREHF 216
Query: 216 KKLYEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIP 275
++ +ED++SI RLNYYP C+ P+L LGTGPHCDPTS+TIL QD VSGLQV VD+QW SIP
Sbjct: 217 REFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQWQSIP 276
Query: 276 PTPDSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVE 335
P P + VVNIGDT MALTNG+YKSC HRAVVN + RK++AFFLCP +K+V+ P +L
Sbjct: 277 PIPQALVVNIGDTLMALTNGIYKSCLHRAVVNGETTRKTLAFFLCPKVDKVVKPPSEL-- 336
Query: 336 KNPPRKFPDFTWPMLHELTQKFYRADSNTLKAFTTWLQQQ 373
R +PDFTW M E T K YRAD NTL+ FT WL+ +
Sbjct: 337 -EGERAYPDFTWSMFLEFTMKHYRADMNTLEEFTNWLKNK 373
BLAST of MC10g0175 vs. TAIR 10
Match:
AT1G44090.1 (gibberellin 20-oxidase 5 )
HSP 1 Score: 299.7 bits (766), Expect = 3.3e-81
Identity = 148/336 (44.05%), Postives = 211/336 (62.80%), Query Frame = 0
Query: 36 PENFIWPDEFKAQDSLPELHVPHIDIKKLLSGDEGGAEEAMRLVDEACRKHGFFVVVNHG 95
P F WP++ A S +L +P ID+ L+G+E + A + V +AC HG F+VVNHG
Sbjct: 44 PAEFFWPEKDVA-PSEGDLDLPIIDLSGFLNGNEAETQLAAKAVKKACMAHGTFLVVNHG 103
Query: 96 VDMGIMKDLHKCMDEFFELPLDQKQKAHRKLGDSYGYTSSYIGRFSDKLPWKETFSVCYL 155
G+ + + FF L D+K +A+R G+ GYT+ + RFS LPW ET ++ +
Sbjct: 104 FKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSNLPWNETLTLAFK 163
Query: 156 SHKDHSSLVHDYVRDTLGQEFSHHGKVYQECCKAMAELSLEVVELLGMSLGL-GREEFKK 215
H +V D++ LG G+V+QE C AM L ++++ELLG+S+GL R +++
Sbjct: 164 KGPPH--VVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLGISMGLKDRTYYRR 223
Query: 216 LYEDHDSIMRLNYYPTCENPELTLGTGPHCDPTSITILHQDHVSGLQVHVDDQWYSIPPT 275
+ED I R NYYP C+ PE LG GPH DPT+IT+L QD V GL+V W ++ P
Sbjct: 224 FFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLEVFAAGSWQTVRPR 283
Query: 276 PDSFVVNIGDTFMALTNGVYKSCFHRAVVNCKEVRKSVAFFLCPGAEKLVRAPEQLVE-K 335
P + VVN+GDTFMAL+NG Y+SC+HRAVVN ++VR+S+ FF CP +K++ P +LVE +
Sbjct: 284 PGALVVNVGDTFMALSNGNYRSCYHRAVVNKEKVRRSLVFFSCPREDKIIVPPPELVEGE 343
Query: 336 NPPRKFPDFTWPMLHELTQKFYRADSNTLKAFTTWL 370
RK+PDFTW L + TQ YR D+ TL F++WL
Sbjct: 344 EASRKYPDFTWAQLQKFTQSGYRVDNTTLHNFSSWL 376
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q39111 | 6.7e-119 | 54.64 | Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1 | [more] |
Q39112 | 5.0e-114 | 54.50 | Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1 | [more] |
Q39110 | 8.5e-114 | 57.49 | Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2 | [more] |
O04707 | 2.3e-111 | 56.34 | Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1 | [more] |
O04706 | 3.0e-111 | 56.64 | Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
XP_022141206.1 | 2.28e-287 | 100.00 | gibberellin 20 oxidase 2-like [Momordica charantia] | [more] |
XP_022141149.1 | 1.74e-245 | 86.33 | gibberellin 20 oxidase 2-like [Momordica charantia] | [more] |
CAA70329.1 | 1.19e-203 | 72.73 | gibberellin 20-oxidase [Marah macrocarpa] | [more] |
XP_022922616.1 | 4.28e-198 | 69.05 | gibberellin 20 oxidase 2-like [Cucurbita moschata] >KAG6576758.1 Gibberellin 20 ... | [more] |
AAD42693.1 | 5.90e-197 | 69.71 | gibberellin 20-oxidase [Citrullus lanatus subsp. vulgaris] | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1CIE9 | 1.10e-287 | 100.00 | gibberellin 20 oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111011662 PE=... | [more] |
A0A6J1CHR5 | 8.42e-246 | 86.33 | gibberellin 20 oxidase 2-like OS=Momordica charantia OX=3673 GN=LOC111011612 PE=... | [more] |
Q96498 | 5.74e-204 | 72.73 | Gibberellin 20-oxidase OS=Marah macrocarpa OX=48242 PE=2 SV=1 | [more] |
A0A6J1E4L1 | 2.07e-198 | 69.05 | gibberellin 20 oxidase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111430571 PE=3... | [more] |
Q9XHR0 | 2.85e-197 | 69.71 | Gibberellin 20-oxidase OS=Citrullus lanatus OX=3654 GN=WGA20OXI PE=2 SV=1 | [more] |