MC00g0475 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC00g0475
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold196: 183455 .. 185431 (-)
RNA-Seq Expression	MC00g0475
Synteny	MC00g0475

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGAAACGGCGGCCCTCGGCGGCGGCATTCGTCTCCCCCAAATTCTCCGCCTCCATTCTCCTCGAATCGCTCCTCGCCCTCTGCCAGGAAATTTCCGCCATGAAACCGCTTCACTTTCTCCTCAAACGCTACTCCAATTCGCTGATTCGGAAGTCCAGATTGCTCGAGATCTTTCTCTACGATCTCCGCCGCAACCGGATTGTTTCCTTCTCCGCCTCCGCCTCCCTCTGCCTCGAAGAAATGTACATCGTCTTGCAGAGAATCAAGACCTTGATCGAAGACTGTTCTAATGGAAGCAAGATGTGGCTCCTCACTCAGAACGAATCCGTCGCCAGTAATTTCCACGAACTCACTCTCGATTTGTCCACTCTACTGGATATTTTCCCCGTCAAGGACGCTGGATTGACTGAAGATGTTGAAGAAATTTTCTACCTCTTGAGAAATCAATGTTCGGAATCAACCGCTTTCGTCGATCCGCGAGACGAAGATCTCCGACGCCGAGTCTCCAACACGATCGATCGGATCAGAGACGAAATCGTCCCCGATCATTCCGAATTATCCGAGATCTTCTCGAGGCTCGATCTTCGCGATTCGTCGAGTTGCAGAGAGGAAATCGAGAATCTCGAGGACGAAGTTCAGAATCAAATCGACGAGAAATCGAAATCCGATGTGAGTGCTATGATCGGACTCGTGAGGTACGCGAAATGCGTTCTGTTCGGAGCGTCGACGCCGGAACCCGGCTTCCGGCGCAAGGATTCCATATCGGATTTGGCTTTGCCGGCGGACTTCAGGTGCCCGATAAGCCTGGACCTAATGCAGGACCCAGTCGTCGTGGCAACGGGACATACGTACGATCGTGCAGCCATAACTCTGTGGATTGAATCTGGGCACAACACGTGTCCGAAGACAGGTCAGAGCCTGGCCCACACGAACCTAATTCCGAACCGCGCATTGAAAAATCTGATAGCGATGTGGTGCCGTCAGGAGAGAATTCCATTCGACGTAACGGAGAATAACAAGGAGAGAGTCAACGGCGTCACGTCGAACAAAGCGGCGTTAGAGGCCATGAGGATGACGGCGTCTTTCCTCGTTAACAAACTCGCCACGTCATCCGAGGACGCGAACGGCGTCGTTCACGAGCTTCGCGTTTTAGCGAAAACGGACTCCGGCAGCCGCGGCTGCATCGCCAAAGCCGGAGCTCTGCCGTTACTCGTCCGTTGTCTCAGCTCCGATGACCCCACCCTCCAAGTTAACGCCGTCACGACGGTCCTCAACCTCTCGATTTACGAGGCGAACAAATCGCTGATAATGGAGACCGACGGCGCGTTGATCGGAGTGATCGAGGTCCTCCGCTCCGGCGCCACGTGGGAGGCGAAAGGAAACGCCGCCGCCACCATATTCAGCCTCTCCAGCGTCCACTCTTACCGGCGGCGATTGGGGAGGAAAGCGCGTGTCGTCAGGGGATTGCTCGATTTGGCCAAGGACGGGCCGATTAATTCGAAGAGGGATGCTCTGGTGACGATTTTGACATTGGCCGGCGACCGGGAGACCGTCGGGAGGCTGATTGAAGGAGGGGTTATGGAGACGGTGAGCCATCTGATGAACAGCCTGCCGGAGGAGGCGGTGACGATTCTGGAAGTGGTGGTGCGGAAAGGGGGATTTGTGGCGATTGCTTCTGGTTTTTATGTGATTAAGAAATTGGGGATTGTTCTGAGGGAGGGTTCCGATAGGGCCAGAGAGAGCGCCGCCGCGGCGCTGGTCACGATGTGCCGTCAAGGCGGGTCGGAGATGGTGTCGGAGCTGGCGTCGATGGCGGGAATTGAGAGAGTGATTTGGGAGCTGATGGGAAGTGGGACGATGAGGGGGCGGCGGAAGGCGGCGTCGCTGTTGAGAATACTCCGGCGATGGGCGGCGGGTTTGGACGGAAACGGCGTCGGAGGAGATTCGATGACCGTTACTTCGTCGAGAATCGGCGGC

mRNA sequence

AGGAAACGGCGGCCCTCGGCGGCGGCATTCGTCTCCCCCAAATTCTCCGCCTCCATTCTCCTCGAATCGCTCCTCGCCCTCTGCCAGGAAATTTCCGCCATGAAACCGCTTCACTTTCTCCTCAAACGCTACTCCAATTCGCTGATTCGGAAGTCCAGATTGCTCGAGATCTTTCTCTACGATCTCCGCCGCAACCGGATTGTTTCCTTCTCCGCCTCCGCCTCCCTCTGCCTCGAAGAAATGTACATCGTCTTGCAGAGAATCAAGACCTTGATCGAAGACTGTTCTAATGGAAGCAAGATGTGGCTCCTCACTCAGAACGAATCCGTCGCCAGTAATTTCCACGAACTCACTCTCGATTTGTCCACTCTACTGGATATTTTCCCCGTCAAGGACGCTGGATTGACTGAAGATGTTGAAGAAATTTTCTACCTCTTGAGAAATCAATGTTCGGAATCAACCGCTTTCGTCGATCCGCGAGACGAAGATCTCCGACGCCGAGTCTCCAACACGATCGATCGGATCAGAGACGAAATCGTCCCCGATCATTCCGAATTATCCGAGATCTTCTCGAGGCTCGATCTTCGCGATTCGTCGAGTTGCAGAGAGGAAATCGAGAATCTCGAGGACGAAGTTCAGAATCAAATCGACGAGAAATCGAAATCCGATGTGAGTGCTATGATCGGACTCGTGAGGTACGCGAAATGCGTTCTGTTCGGAGCGTCGACGCCGGAACCCGGCTTCCGGCGCAAGGATTCCATATCGGATTTGGCTTTGCCGGCGGACTTCAGGTGCCCGATAAGCCTGGACCTAATGCAGGACCCAGTCGTCGTGGCAACGGGACATACGTACGATCGTGCAGCCATAACTCTGTGGATTGAATCTGGGCACAACACGTGTCCGAAGACAGGTCAGAGCCTGGCCCACACGAACCTAATTCCGAACCGCGCATTGAAAAATCTGATAGCGATGTGGTGCCGTCAGGAGAGAATTCCATTCGACGTAACGGAGAATAACAAGGAGAGAGTCAACGGCGTCACGTCGAACAAAGCGGCGTTAGAGGCCATGAGGATGACGGCGTCTTTCCTCGTTAACAAACTCGCCACGTCATCCGAGGACGCGAACGGCGTCGTTCACGAGCTTCGCGTTTTAGCGAAAACGGACTCCGGCAGCCGCGGCTGCATCGCCAAAGCCGGAGCTCTGCCGTTACTCGTCCGTTGTCTCAGCTCCGATGACCCCACCCTCCAAGTTAACGCCGTCACGACGGTCCTCAACCTCTCGATTTACGAGGCGAACAAATCGCTGATAATGGAGACCGACGGCGCGTTGATCGGAGTGATCGAGGTCCTCCGCTCCGGCGCCACGTGGGAGGCGAAAGGAAACGCCGCCGCCACCATATTCAGCCTCTCCAGCGTCCACTCTTACCGGCGGCGATTGGGGAGGAAAGCGCGTGTCGTCAGGGGATTGCTCGATTTGGCCAAGGACGGGCCGATTAATTCGAAGAGGGATGCTCTGGTGACGATTTTGACATTGGCCGGCGACCGGGAGACCGTCGGGAGGCTGATTGAAGGAGGGGTTATGGAGACGGTGAGCCATCTGATGAACAGCCTGCCGGAGGAGGCGGTGACGATTCTGGAAGTGGTGGTGCGGAAAGGGGGATTTGTGGCGATTGCTTCTGGTTTTTATGTGATTAAGAAATTGGGGATTGTTCTGAGGGAGGGTTCCGATAGGGCCAGAGAGAGCGCCGCCGCGGCGCTGGTCACGATGTGCCGTCAAGGCGGGTCGGAGATGGTGTCGGAGCTGGCGTCGATGGCGGGAATTGAGAGAGTGATTTGGGAGCTGATGGGAAGTGGGACGATGAGGGGGCGGCGGAAGGCGGCGTCGCTGTTGAGAATACTCCGGCGATGGGCGGCGGGTTTGGACGGAAACGGCGTCGGAGGAGATTCGATGACCGTTACTTCGTCGAGAATCGGCGGC

Coding sequence (CDS)

AGGAAACGGCGGCCCTCGGCGGCGGCATTCGTCTCCCCCAAATTCTCCGCCTCCATTCTCCTCGAATCGCTCCTCGCCCTCTGCCAGGAAATTTCCGCCATGAAACCGCTTCACTTTCTCCTCAAACGCTACTCCAATTCGCTGATTCGGAAGTCCAGATTGCTCGAGATCTTTCTCTACGATCTCCGCCGCAACCGGATTGTTTCCTTCTCCGCCTCCGCCTCCCTCTGCCTCGAAGAAATGTACATCGTCTTGCAGAGAATCAAGACCTTGATCGAAGACTGTTCTAATGGAAGCAAGATGTGGCTCCTCACTCAGAACGAATCCGTCGCCAGTAATTTCCACGAACTCACTCTCGATTTGTCCACTCTACTGGATATTTTCCCCGTCAAGGACGCTGGATTGACTGAAGATGTTGAAGAAATTTTCTACCTCTTGAGAAATCAATGTTCGGAATCAACCGCTTTCGTCGATCCGCGAGACGAAGATCTCCGACGCCGAGTCTCCAACACGATCGATCGGATCAGAGACGAAATCGTCCCCGATCATTCCGAATTATCCGAGATCTTCTCGAGGCTCGATCTTCGCGATTCGTCGAGTTGCAGAGAGGAAATCGAGAATCTCGAGGACGAAGTTCAGAATCAAATCGACGAGAAATCGAAATCCGATGTGAGTGCTATGATCGGACTCGTGAGGTACGCGAAATGCGTTCTGTTCGGAGCGTCGACGCCGGAACCCGGCTTCCGGCGCAAGGATTCCATATCGGATTTGGCTTTGCCGGCGGACTTCAGGTGCCCGATAAGCCTGGACCTAATGCAGGACCCAGTCGTCGTGGCAACGGGACATACGTACGATCGTGCAGCCATAACTCTGTGGATTGAATCTGGGCACAACACGTGTCCGAAGACAGGTCAGAGCCTGGCCCACACGAACCTAATTCCGAACCGCGCATTGAAAAATCTGATAGCGATGTGGTGCCGTCAGGAGAGAATTCCATTCGACGTAACGGAGAATAACAAGGAGAGAGTCAACGGCGTCACGTCGAACAAAGCGGCGTTAGAGGCCATGAGGATGACGGCGTCTTTCCTCGTTAACAAACTCGCCACGTCATCCGAGGACGCGAACGGCGTCGTTCACGAGCTTCGCGTTTTAGCGAAAACGGACTCCGGCAGCCGCGGCTGCATCGCCAAAGCCGGAGCTCTGCCGTTACTCGTCCGTTGTCTCAGCTCCGATGACCCCACCCTCCAAGTTAACGCCGTCACGACGGTCCTCAACCTCTCGATTTACGAGGCGAACAAATCGCTGATAATGGAGACCGACGGCGCGTTGATCGGAGTGATCGAGGTCCTCCGCTCCGGCGCCACGTGGGAGGCGAAAGGAAACGCCGCCGCCACCATATTCAGCCTCTCCAGCGTCCACTCTTACCGGCGGCGATTGGGGAGGAAAGCGCGTGTCGTCAGGGGATTGCTCGATTTGGCCAAGGACGGGCCGATTAATTCGAAGAGGGATGCTCTGGTGACGATTTTGACATTGGCCGGCGACCGGGAGACCGTCGGGAGGCTGATTGAAGGAGGGGTTATGGAGACGGTGAGCCATCTGATGAACAGCCTGCCGGAGGAGGCGGTGACGATTCTGGAAGTGGTGGTGCGGAAAGGGGGATTTGTGGCGATTGCTTCTGGTTTTTATGTGATTAAGAAATTGGGGATTGTTCTGAGGGAGGGTTCCGATAGGGCCAGAGAGAGCGCCGCCGCGGCGCTGGTCACGATGTGCCGTCAAGGCGGGTCGGAGATGGTGTCGGAGCTGGCGTCGATGGCGGGAATTGAGAGAGTGATTTGGGAGCTGATGGGAAGTGGGACGATGAGGGGGCGGCGGAAGGCGGCGTCGCTGTTGAGAATACTCCGGCGATGGGCGGCGGGTTTGGACGGAAACGGCGTCGGAGGAGATTCGATGACCGTTACTTCGTCGAGAATCGGCGGC

Protein sequence

RKRRPSAAAFVSPKFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLYDLRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLDLSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIVPDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKCVLFGASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTCPKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTASFLVNKLATSSEDANGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQVNAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRRRLGRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSLPEEAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEMVSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNGVGGDSMTVTSSRIGG

Homology

[Close]

BLAST of MC00g0475 vs. ExPASy Swiss-Prot
Match: Q9LZW3 (U-box domain-containing protein 16 OS=Arabidopsis thaliana OX=3702 GN=PUB16 PE=2 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 2.7e-208
Identity = 390/664 (58.73%), Postives = 501/664 (75.45%), Query Frame = 0


Query: 1   RKRRP-SAAAFVSPKFSASI-LLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIF 60
           RKRRP    +F SPK S+   L  SL     EIS+M+PL F+L+R S SLIRK ++L   
Sbjct: 12  RKRRPLVVGSFESPKLSSDTKLTRSLFLASHEISSMQPLPFILRRNSLSLIRKVKILASV 71

Query: 61  LYD--LRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHE 120
             +  L R+++V +S SA LC EEM IV+QRIK+LI+DCS  SK+WLL Q + VA NFHE
Sbjct: 72  FDELLLPRSQLVVYSQSAHLCFEEMQIVMQRIKSLIDDCSRVSKLWLLLQIDIVAFNFHE 131

Query: 121 LTLDLSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIR 180
           L  DLST+LDI P+ D  L++D +++  LL  QCS+S  FVD RD  LRR+V++TI  I+
Sbjct: 132 LVTDLSTVLDILPLHDFDLSDDAQDLISLLTKQCSDSVQFVDARDVALRRKVTDTIAGIK 191

Query: 181 DEIVPDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKC 240
            +I PDHS L +IF+ L L DS+S  +EI+ LEDE+Q+QID++SKS  +++IGLVRY+KC
Sbjct: 192 HQISPDHSTLIKIFNDLGLSDSASLTDEIQRLEDEIQDQIDDRSKSAAASLIGLVRYSKC 251

Query: 241 VLFGASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESG 300
           VL+G STP P FRR  S+SD  +PADFRCPI+L+LM+DPVVVATG TYDR +I LWI+SG
Sbjct: 252 VLYGPSTPAPDFRRHQSLSDANIPADFRCPITLELMRDPVVVATGQTYDRESIDLWIQSG 311

Query: 301 HNTCPKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAM 360
           HNTCPKTGQ L HT+L+PNRALKNLI +WCR ++IPF++  +           K A+E  
Sbjct: 312 HNTCPKTGQVLKHTSLVPNRALKNLIVLWCRDQKIPFELYGDGG---GEPAPCKEAVEFT 371

Query: 361 RMTASFLVNKLATSSEDANGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQ 420
           +M  SFL+ KL+ +  D+NGVV ELR LAK+D+ +R CIA+AGA+P LVR L+++ P+LQ
Sbjct: 372 KMMVSFLIEKLSVA--DSNGVVFELRALAKSDTVARACIAEAGAIPKLVRYLATECPSLQ 431

Query: 421 VNAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYR 480
           +NAVTT+LNLSI E NK+ IMETDGAL GVIEVLRSGATWEAK NAAAT+FSL+ V +YR
Sbjct: 432 INAVTTILNLSILEQNKTRIMETDGALNGVIEVLRSGATWEAKANAAATLFSLAGVSAYR 491

Query: 481 RRLGRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNS 540
           RRLGRKARVV GL+DLAK GP +SKRDALV IL L  +RE VGR +E GVM         
Sbjct: 492 RRLGRKARVVSGLVDLAKQGPTSSKRDALVAILNLVAERENVGRFVEAGVMGAAGDAFQE 551

Query: 541 LPEEAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSE 600
           LPEEAV ++E VVR+GG +A+++ F +I+ LG V+REG+D  RESAAA LVTMCR+GGSE
Sbjct: 552 LPEEAVAVVEAVVRRGGLMAVSAAFSLIRLLGEVMREGADTTRESAAATLVTMCRKGGSE 611

Query: 601 MVSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNGVGGDSMTV---T 658
           +V+E+A++ GIERVIWE++G+GT RG RKAASL+R LRRWAAG D +    ++ ++   T
Sbjct: 612 LVAEMAAIPGIERVIWEMIGAGTARGGRKAASLMRYLRRWAAG-DTHNTAAETQSIVVPT 669

[Close]

BLAST of MC00g0475 vs. ExPASy Swiss-Prot
Match: Q9C7R6 (U-box domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=PUB17 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 3.0e-114
Identity = 270/701 (38.52%), Postives = 397/701 (56.63%), Query Frame = 0


Query: 1   RKRRPSAAAFVSP-KFSASILLESLLALCQE-ISAMKPLHFLLKR-YSNSLIRKSRLLEI 60
           R+R PS  AF++P   S   L+++L ++  E +S    + F  +R  + SLIRK  +  +
Sbjct: 12  RRRSPSLEAFLAPVDLSGVALVQTLASISSEVVSCFTSVRFSFQRKNARSLIRKIEIFVV 71

Query: 61  ---FLYD------------LRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMW 120
              FL D             RR++     ++A LCL+E+Y++L R K L++ C+  SK+W
Sbjct: 72  LFEFLVDSNWGSTTTRTRARRRSKSSVSESTALLCLKELYLLLYRSKILVDYCAQSSKLW 131

Query: 121 LLTQNESVASNFHELTLDLSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDE 180
           LL QN S++  FH+L  ++STLLD+ PV D GL++D+ E   LL+ Q  ++  ++D  DE
Sbjct: 132 LLLQNPSISGYFHDLNQEISTLLDVLPVNDLGLSDDIREQIELLQRQSRKARLYIDKNDE 191

Query: 181 DLRRRVSNTIDRIRDEIVPDHSELSEIF-SRLDLRDSSSCREEIENLEDEVQNQID--EK 240
            LR    + +D   +  +P   +L   F  +L +RDS SCR EIE LE+++ N     E 
Sbjct: 192 SLRESFYSFLDGFENGKIPSSVDLRMFFVEKLGIRDSKSCRSEIEFLEEQIVNHDGDLEP 251

Query: 241 SKSDVSAMIGLVRYAKCVLFG-------------ASTPEPGFRRKDSISD--LALPADFR 300
           + S ++  + + RY + +LFG                P  GF  ++ I D  + +P DF 
Sbjct: 252 TGSVINGFVAITRYCRFLLFGFEEDGMEWWIENNPKKPRKGFVAQE-IGDTFITVPKDFV 311

Query: 301 CPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTCPKTGQSLAHTNLIPNRALKNLIAM 360
           CPISLDLM DPV+++TG TYDR +I  WIE GH TCPKTGQ L  + ++PNRALKNLI  
Sbjct: 312 CPISLDLMTDPVIISTGQTYDRNSIARWIEEGHCTCPKTGQMLMDSRIVPNRALKNLIVQ 371

Query: 361 WCRQERIPF--DVTENNKERVNGVTSNKAALEAMRMTASFLVNKLATSSEDANGV-VHEL 420
           WC    I +  + T++  E        KAA+EA + T S L+  LA  S+ A  V   E+
Sbjct: 372 WCTASGISYESEFTDSPNESFASALPTKAAVEANKATVSILIKYLADGSQAAQTVAAREI 431

Query: 421 RVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQVNAVTTVLNLSIYEANKSLIMETDG 480
           R+LAKT   +R  IA+AGA+P L R L+S++   Q N+VT +LNLSIYE NKS IME   
Sbjct: 432 RLLAKTGKENRAYIAEAGAIPHLCRLLTSENAIAQENSVTAMLNLSIYEKNKSRIMEEGD 491

Query: 481 ALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRRRLGRKARVVRGLLDLAKDGPINSK 540
            L  ++ VL SG T EA+ NAAAT+FSLS+VH Y++R+    + V  L  L ++G    K
Sbjct: 492 CLESIVSVLVSGLTVEAQENAAATLFSLSAVHEYKKRIAIVDQCVEALALLLQNGTPRGK 551

Query: 541 RDALVTILTLAGDRETVGRLIE-GGVMETVSHLMN-SLPEEAVTILEVVVRKG-GFVAIA 600
           +DA+  +  L+   +   R+IE GGV   V  L N  + EEA   L ++VR+  G  AI 
Sbjct: 552 KDAVTALYNLSTHPDNCSRMIEGGGVSSLVGALKNEGVAEEAAGALALLVRQSLGAEAIG 611

Query: 601 SGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEMVSELASMAGIERVIWELMGSG 660
                +  L  ++R G+ R +E+A AAL+ +CR GG+ +  ++     I  ++  L+ +G
Sbjct: 612 KEDSAVAGLMGMMRCGTPRGKENAVAALLELCRSGGAAVAEKVLRAPAIAGLLQTLLFTG 671

[Close]

BLAST of MC00g0475 vs. ExPASy Swiss-Prot
Match: Q6EUK7 (U-box domain-containing protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB4 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.0e-97
Identity = 229/662 (34.59%), Postives = 376/662 (56.80%), Query Frame = 0


Query: 1   RKRRPSAAAFVSPK-FSASILLESLLALCQEI--SAMKPLHFLLKRYSNSLIRKSRLLEI 60
           R+R P A AF +P   + + LL ++ +L   +   A  P     +R  ++L R+  LL  
Sbjct: 22  RRRLPLAGAFFAPTGLAGATLLRAVASLAASLVAGARPPSQ---RRNVDALARRLALLSA 81

Query: 61  FLYDLRRNRIV--SFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFH 120
            L  +  +     +FS +A+LC  E+Y+VL R + L+   ++  + W L ++  +A++F 
Sbjct: 82  ILESILLDTAAAGAFSDAANLCFRELYVVLFRAELLVSYVASAGRAWALLRSPHLAASFR 141

Query: 121 ELTLDLSTLLDIFPVKDAGLTEDVEEIFYLLRN--QCSESTAFVDPRDEDLRRRVSNTID 180
           +L  +L+ +LD+ P     L+ D   +  LLR   +C     + DP +  LR R+ + + 
Sbjct: 142 DLDAELAVVLDVLPAASLRLSHDATGLLDLLRAHCRCRAPAQYHDPDEAALRERLMDALR 201

Query: 181 RIRDEIVPDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRY 240
           +      PDH  L  + + + +  ++SCR EI+ LE+++ +Q ++     V +++ L+RY
Sbjct: 202 QFDLGQPPDHPSLQSLLADMGISTAASCRAEIDYLEEQILSQEEDTDLPLVGSVLALLRY 261

Query: 241 AKCVLFGAST-------PEPGFRRK------DSISDLALPADFRCPISLDLMQDPVVVAT 300
               +F  S        P  G R++         +  ++P +F CPISLDLM+DPVV +T
Sbjct: 262 CLFAVFDPSNAKALRDWPLSGNRQRLLSIGGGDDTSFSVPKEFSCPISLDLMRDPVVAST 321

Query: 301 GHTYDRAAITLWIESGHNTCPKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNK 360
           G TYDR +I  WIE GH+TCP +GQ+LA   L+PNRAL++LI+ WC    + +D  E+N+
Sbjct: 322 GQTYDRPSIIQWIEEGHSTCPNSGQTLADHRLVPNRALRSLISQWCGVYGLQYDSPESNE 381

Query: 361 ---ERVNGVTSNKAALEAMRMTASFLVNKLATSSEDANGV-VHELRVLAKTDSGSRGCIA 420
              E V    S++AA+EA + TA  LV  L   SE+   V   E+R+LAKT   +R  IA
Sbjct: 382 GMAECVAASCSSRAAMEANKATARILVRMLEDGSENVKAVAAKEIRLLAKTGKQNRAFIA 441

Query: 421 KAGALPLLVRCLSSDDPTLQVNAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATW 480
             GA+PLL R L S+D   Q NAVT +LNLSI+E NK  IME +G L  ++ VL++G T 
Sbjct: 442 DLGAIPLLCRLLLSNDWMAQENAVTALLNLSIFEPNKGRIMEQEGCLRLIVGVLQNGWTT 501

Query: 481 EAKGNAAATIFSLSSVHSYRRRLGRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRE 540
           EAK NAAAT+FSLS VH++++ +  +   V  L  +   G    K+DA++ +  L+   E
Sbjct: 502 EAKENAAATLFSLSVVHNFKKLIMNEPGAVEELASMLTKGTSRGKKDAVMALFNLSTHPE 561

Query: 541 TVGRLIEG-GVMETVSHLMN-SLPEEAVTILEVVVRKGGFV-AIASGFYVIKKLGIVLRE 600
           +  R++E   V+  +  L N ++ EEA   L +++++   V  + S   VI  L  ++R 
Sbjct: 562 SSARMLESCAVVALIQSLRNDTVSEEAAGALALLMKQPSIVHLVGSSETVITSLVGLMRR 621

Query: 601 GSDRARESAAAALVTMCRQGGSEMVSELASMAGIERVIWELMGSGTMRGRRKAASLLRIL 636
           G+ + +E+A +AL  +CR+GGS +V  +A + G+  VI  +  +GT R ++KA+ ++++ 
Sbjct: 622 GTPKGKENAVSALYEICRRGGSALVQRVAKIPGLNTVIQTITLNGTKRAKKKASLIVKMC 680

[Close]

BLAST of MC00g0475 vs. ExPASy Swiss-Prot
Match: E4NKF8 (U-box domain-containing protein 1 OS=Medicago truncatula OX=3880 GN=PUB1 PE=1 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.0e-89
Identity = 224/686 (32.65%), Postives = 365/686 (53.21%), Query Frame = 0


Query: 5   PSAAAFVSP-KFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLYDLR 64
           P +   +SP       LL+SL+ +  E+S+M+    +  +  +S+IR+ +LL     +++
Sbjct: 4   PRSKMMISPGLLPTESLLDSLILISNEVSSMQKFPLVQIKNVSSMIRRIKLLSSLFEEIQ 63

Query: 65  RNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLDLST 124
            +       S+ LC  E++ V+ R+K LI++C++GS +W L Q + +++ F  L  ++  
Sbjct: 64  ESD-SPLPPSSILCFIEIFSVITRVKVLIQECTDGSSLWSLIQLDFISNQFFVLVKEMGR 123

Query: 125 LLDIFPVKDAGLTEDVEEIFYLLRNQCS--ESTAFVDPRDEDLRRRVSNTIDR------- 184
            LDI P+    + +D++E   LL  Q    E   F+DPR+   R  +   + +       
Sbjct: 124 ALDILPLNLLNVAQDIKEQVDLLHKQSKRVELELFIDPREVQRRENLFEVMSKNCLQNKK 183

Query: 185 -IRDEIVPDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKS---KSDVSAMIGL 244
              ++   D  ++ EI   + LR  S   EEI  LE E QNQ         S+++ ++ L
Sbjct: 184 TNNNKGFIDFVKVEEIMCSIGLRTLSDYVEEISKLEVEAQNQAGTGGLIVVSNINNLMSL 243

Query: 245 VRYAKCVLF------------------------GASTPEPGFRRKDSISDLALPADFRCP 304
           V Y K ++F                          S+    F    S+  + +P +FRCP
Sbjct: 244 VSYTKSMVFRNDGESEECKPISMFLYNKSKIHDNDSSSSSSF--SQSMMTVNIPDEFRCP 303

Query: 305 ISLDLMQDPVVVATGHTYDRAAITLWIESGHNTCPKTGQSLAHTNLIPNRALKNLIAMWC 364
           ISLDLM+DPV+V++GHTYDR +I  WI SGH+TCPK+GQ L HT LIPN ALK+L+  WC
Sbjct: 304 ISLDLMRDPVIVSSGHTYDRISIAEWINSGHHTCPKSGQRLIHTALIPNYALKSLVHQWC 363

Query: 365 RQERIPFD--VTEN---------NKERVNGVTSNKAALEAMRMTASFLVNKLATSSED-A 424
            +  +  +  +T+N         N+  ++ ++ NKA+ +A++MTA FLV KLAT S D  
Sbjct: 364 YENNVKMNEAITKNNNSSSKRHKNENAIDHISENKASKDAVKMTAEFLVGKLATGSTDIQ 423

Query: 425 NGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQVNAVTTVLNLSIYEANKS 484
               +E+R+LAKT   +R  IA+ GA+P LV  L S D  +Q + VT + NLSIY+ NK 
Sbjct: 424 RQSAYEIRLLAKTGMDNRRIIAEVGAIPFLVTLLVSKDSRIQEHVVTALFNLSIYDNNKI 483

Query: 485 LIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRRRLGRKARVVRGLLDLAK 544
           LIM   GA+  ++EVL  G T EA+ NAAA I+SLS +   + ++G  +R +  L+ L K
Sbjct: 484 LIMAA-GAIDNIVEVLEFGKTMEARENAAAAIYSLSMIDDCKVQIGASSRAIPALVGLLK 543

Query: 545 DGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLM----NSLPEEAVTILEVVVR 604
           +G I  KRDA   +  LA        +++ G +  +  L+      + ++++ +L V++ 
Sbjct: 544 EGTIIGKRDAATALFNLAVYNPNKLSIVKSGAVTLLVELLMDDKAGITDDSLAVLAVLLG 603

Query: 605 -KGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEMVSELASMAGIER 636
              G   I +   ++  L  +LR GS + +E++   L+ +C++ G  +   L +      
Sbjct: 604 CSEGLEEIKNSKSLVPLLIDLLRFGSVKGKENSITLLLGLCKEEGELVAMRLLANPRSIP 663

[Close]

BLAST of MC00g0475 vs. ExPASy Swiss-Prot
Match: O80742 (U-box domain-containing protein 19 OS=Arabidopsis thaliana OX=3702 GN=PUB19 PE=2 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.6e-75
Identity = 210/663 (31.67%), Postives = 360/663 (54.30%), Query Frame = 0


Query: 16  SASILLESLLALCQEISAMKPLHFLL-KRYSNSLIRKSRLLEIFLYDLR-RNRIVSFSAS 75
           S + L++SLL L  EI + KP HF   KR     +R  + L IF  +LR + R+ S  A 
Sbjct: 25  SLTTLVDSLLQLAGEILSFKPKHFSTNKRSVKETLRHVQTLVIFFEELRIQIRVGSIPAG 84

Query: 76  AS--LCLEEMYIVLQRIKTLIEDCS-NGSKMWLLTQNESVASNFHELTLDLSTLLDIFPV 135
            S  L L E++++ Q++K L++DC+ +G+K+++L  +  V+++F +LT  +ST LD FPV
Sbjct: 85  RSVILSLSELHVIFQKLKFLLDDCTRDGAKLYMLMNSGQVSAHFRDLTRSISTSLDTFPV 144

Query: 136 KDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIVPDHSELSEIF 195
           +   L  +V E+ YL+  Q  +S A  D  D+     V    +   + I P+  E+  + 
Sbjct: 145 RSVDLPGEVNELIYLVMRQTRKSEARPDRDDKRAIDSVYWFFNLFENRINPNSDEILRVL 204

Query: 196 SRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDV---SAMIGLVRYAKCVLFGASTPEPG 255
             + +R    C +EI+ L +E+   + +KS  ++   S ++G + Y +CV+      +  
Sbjct: 205 DHIGVRKWRDCVKEIDFLREEI--SVGKKSNIEIELLSNLMGFICYCRCVILRGIDVDDE 264

Query: 256 FRRKDSISDLALPA-----DFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTCPK 315
            + K+   DL +       D RCPISL++M DPVV+ +GHTYDR++IT W  SG+ TCPK
Sbjct: 265 EKDKEE-DDLMMVRSLNVDDLRCPISLEIMSDPVVLESGHTYDRSSITKWFASGNITCPK 324

Query: 316 TGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTASF 375
           TG++L  T L+ N ++K +I  + +Q  +   + +  K++V+ V  + AA EA ++TA F
Sbjct: 325 TGKTLVSTVLVDNFSVKQVIQSYSKQNGVV--MGQKGKKKVD-VAESLAAEEAGKLTAEF 384

Query: 376 LVNKLATSSEDAN-GVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQVNAVT 435
           L  +L    E+     + E+R+L KT +  R C+ +AG +  L++ L SDDP +Q NA+ 
Sbjct: 385 LAGELIKGDEEEMVKALVEIRILTKTSTFYRSCLVEAGVVESLMKILRSDDPRIQENAMA 444

Query: 436 TVLNLSIYEANKSLIM-ETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRRRLG 495
            ++NLS   A K+ I+ E  G L  ++EVL  GA  E++  AAA +F LSS+  Y R +G
Sbjct: 445 GIMNLSKDIAGKTRIVGEDGGGLRLIVEVLNDGARRESRQYAAAALFYLSSLGDYSRLIG 504

Query: 496 RKARVVRGLLDLAK--DGPINSKRDALVTILTLAGDR-ETVGRLIEGGVMETVSHLMNS- 555
             +  + GL+ + K  D   ++KR+AL+ I +L  ++ +   R++  G++  +  L+ S 
Sbjct: 505 EISDAIPGLVRIVKSCDYGDSAKRNALIAIRSLLMNQPDNHWRILAAGIVPVLLDLVKSE 564

Query: 556 -----LPEEAVTILE----------VVVRKGGFVAIASGFYVIKKLGIVLREGSDRARES 615
                +  +++ IL            V+R+GG          +K LG    E S   ++ 
Sbjct: 565 EISDGVTADSMAILAKMAEYPDGMISVLRRGGLK------LAVKILG--SSEVSPATKQH 624

Query: 616 AAAALVTMCRQGGSEMVSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLD 645
             A L+ +C  GGS++V  LA    I   ++    +G + G +KA++L++++  +     
Sbjct: 625 CVALLLNLCHNGGSDVVGSLAKNPSIMGSLYTASSNGELGGGKKASALIKMIHEFQERKT 673

[Close]

BLAST of MC00g0475 vs. NCBI nr
Match: XP_022925177.1 (U-box domain-containing protein 16-like [Cucurbita moschata])

HSP 1 Score: 1118 bits (2892), Expect = 0.0
Identity = 590/659 (89.53%), Postives = 620/659 (94.08%), Query Frame = 0


Query: 1   RKRRPSAAAFVSPKFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLY 60
           RKRRPSAAAFVSP FSA  LL+SLL LCQEISAMKPLHFLL RYSNS+IRKSRLLE+ L 
Sbjct: 11  RKRRPSAAAFVSPNFSAQALLDSLLCLCQEISAMKPLHFLLNRYSNSMIRKSRLLEVILE 70

Query: 61  DLRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLD 120
           D RR+RIVS SASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNES+A+NFHELTLD
Sbjct: 71  DFRRDRIVSLSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESIANNFHELTLD 130

Query: 121 LSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIV 180
           LSTLLDIFPVKDAGLTEDVEE+FYLLRN CSES+AFVDPRDEDLR RV  TIDRIRDEIV
Sbjct: 131 LSTLLDIFPVKDAGLTEDVEELFYLLRNHCSESSAFVDPRDEDLRFRVLKTIDRIRDEIV 190

Query: 181 PDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKCVLFG 240
           PD++ELSEIFSR+DLRDSSSCREEIENLEDEVQNQ DEKS+SDV A+IG VRYAKCVL+G
Sbjct: 191 PDYTELSEIFSRIDLRDSSSCREEIENLEDEVQNQTDEKSRSDVIALIGFVRYAKCVLYG 250

Query: 241 ASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTC 300
           ASTPE GF+RKDSISDLALPADFRCPISLDLM DPVVVATGHTYDRAAITLWIESGHNTC
Sbjct: 251 ASTPESGFQRKDSISDLALPADFRCPISLDLMHDPVVVATGHTYDRAAITLWIESGHNTC 310

Query: 301 PKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTA 360
           PKTGQ+LAHT+LIPNRALKNLIAMWCRQERIPFDVTE+NK  +NGVT NKAALEAMRMTA
Sbjct: 311 PKTGQTLAHTDLIPNRALKNLIAMWCRQERIPFDVTESNKGGINGVTLNKAALEAMRMTA 370

Query: 361 SFLVNKLAT---SSEDANGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQV 420
           SFLVNKLAT   S  DANGVV+ELRVLAKTDSGSRG IA+AGALPLLVR L SDDPTLQV
Sbjct: 371 SFLVNKLATPLDSVNDANGVVYELRVLAKTDSGSRGFIAQAGALPLLVRHLISDDPTLQV 430

Query: 421 NAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 480
           NAVTTVLNLSI EANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR
Sbjct: 431 NAVTTVLNLSILEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 490

Query: 481 RLGRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSL 540
           RLGRK RV+RGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSH+MNSL
Sbjct: 491 RLGRKTRVIRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHVMNSL 550

Query: 541 PEEAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEM 600
           PEEAVTILE+VVRKGGFVAIAS F +IKKLG VLREGSDRARESAAAALVTMCRQGGS+M
Sbjct: 551 PEEAVTILEMVVRKGGFVAIASAFNLIKKLGTVLREGSDRARESAAAALVTMCRQGGSDM 610

Query: 601 VSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNG-VGGDSMTVTSS 655
           V+ELASMAGIERVIWE+MGSGTMRGRRKAASLLRILRRWAAGLD NG VGG  +T+ SS
Sbjct: 611 VTELASMAGIERVIWEMMGSGTMRGRRKAASLLRILRRWAAGLDRNGGVGGGDLTMVSS 669

[Close]

BLAST of MC00g0475 vs. NCBI nr
Match: KAG6595558.1 (U-box domain-containing protein 16, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027538.1 U-box domain-containing protein 16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1115 bits (2883), Expect = 0.0
Identity = 588/659 (89.23%), Postives = 620/659 (94.08%), Query Frame = 0


Query: 1   RKRRPSAAAFVSPKFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLY 60
           RKRRPSAAAFVSP FSA  LL+SLL LCQEISAMKPLHFLL RYSNS+IRKSRLLE+ L 
Sbjct: 11  RKRRPSAAAFVSPNFSAQALLDSLLCLCQEISAMKPLHFLLNRYSNSMIRKSRLLEVILE 70

Query: 61  DLRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLD 120
           D RR+RIVS SASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNES+A+NFHELTLD
Sbjct: 71  DFRRDRIVSLSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESIANNFHELTLD 130

Query: 121 LSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIV 180
           LSTLLDIFPVKDAGLTEDVEE+FYLLRN CSES+AFVDPRDEDLR RV  TID+IRDEIV
Sbjct: 131 LSTLLDIFPVKDAGLTEDVEELFYLLRNHCSESSAFVDPRDEDLRFRVLKTIDQIRDEIV 190

Query: 181 PDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKCVLFG 240
           PD++ELSEIFSR+DLRDSSSCREEIENLEDEVQNQ DEKS+SDV A+IG VRYAKCVL+G
Sbjct: 191 PDYTELSEIFSRIDLRDSSSCREEIENLEDEVQNQTDEKSRSDVIALIGFVRYAKCVLYG 250

Query: 241 ASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTC 300
           ASTPE GF+RKDSISDLALPADFRCPISLDLM DPVVVATGHTYDRAAITLWIESGHNTC
Sbjct: 251 ASTPESGFQRKDSISDLALPADFRCPISLDLMHDPVVVATGHTYDRAAITLWIESGHNTC 310

Query: 301 PKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTA 360
           PKTGQ+LAHT+LIPNRALKNLIAMWCRQERIPFDVTE+NK  +NGVT NKAALEAMRMTA
Sbjct: 311 PKTGQTLAHTDLIPNRALKNLIAMWCRQERIPFDVTESNKGGINGVTLNKAALEAMRMTA 370

Query: 361 SFLVNKLAT---SSEDANGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQV 420
           SFLVNKLAT   S  DANGVV+ELRVLAKTDSGSRG IA+AGALPLLVR L SDDPTLQV
Sbjct: 371 SFLVNKLATPLDSVNDANGVVYELRVLAKTDSGSRGFIAQAGALPLLVRHLISDDPTLQV 430

Query: 421 NAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 480
           NAVTTVLNLSI EANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR
Sbjct: 431 NAVTTVLNLSILEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 490

Query: 481 RLGRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSL 540
           RLGRK RV+RGLLDLAKDGPI+SKRDALVTILTLAGDRETVGRLIEGGVMETVSH+MNSL
Sbjct: 491 RLGRKTRVIRGLLDLAKDGPISSKRDALVTILTLAGDRETVGRLIEGGVMETVSHVMNSL 550

Query: 541 PEEAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEM 600
           PEEAVTILE+VVRKGGFVAIAS F +IKKLG VLREGSDRARESAAAALVTMCRQGGS+M
Sbjct: 551 PEEAVTILEMVVRKGGFVAIASAFNLIKKLGAVLREGSDRARESAAAALVTMCRQGGSDM 610

Query: 601 VSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNG-VGGDSMTVTSS 655
           V+ELASMAGIERVIWE+MGSGTMRGRRKAASLLRILRRWAAGLD NG VGG  +T+ SS
Sbjct: 611 VTELASMAGIERVIWEMMGSGTMRGRRKAASLLRILRRWAAGLDRNGGVGGGDLTMVSS 669

[Close]

BLAST of MC00g0475 vs. NCBI nr
Match: XP_023521732.1 (U-box domain-containing protein 16-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1114 bits (2881), Expect = 0.0
Identity = 588/659 (89.23%), Postives = 620/659 (94.08%), Query Frame = 0


Query: 1   RKRRPSAAAFVSPKFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLY 60
           RKRRPSAAAFVSP FSA  LL+SLL+LCQEISAMKPLHFLL RYSNS+IRKSRLLE+ L 
Sbjct: 11  RKRRPSAAAFVSPNFSAQALLDSLLSLCQEISAMKPLHFLLNRYSNSMIRKSRLLEVILE 70

Query: 61  DLRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLD 120
           D RR+RIVS SASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNES+A+NFHELTLD
Sbjct: 71  DFRRDRIVSLSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESIANNFHELTLD 130

Query: 121 LSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIV 180
           LSTLLDIFPVKDAGLTEDVEE+FYLLRN CSES+AFVDPRDEDLR RV  TIDRIRDEIV
Sbjct: 131 LSTLLDIFPVKDAGLTEDVEELFYLLRNHCSESSAFVDPRDEDLRFRVLKTIDRIRDEIV 190

Query: 181 PDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKCVLFG 240
           PD++ELSEIFSR+DLRDSSSCREEIENLEDEVQNQ DEKS+SDV A+IG VRYAKCVL+G
Sbjct: 191 PDYTELSEIFSRIDLRDSSSCREEIENLEDEVQNQTDEKSRSDVIALIGFVRYAKCVLYG 250

Query: 241 ASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTC 300
           ASTP  GF+RKDSISDLALPADFRCPISLDLM DPVVVATGHTYDRAAITLWIESGHNTC
Sbjct: 251 ASTPGSGFQRKDSISDLALPADFRCPISLDLMHDPVVVATGHTYDRAAITLWIESGHNTC 310

Query: 301 PKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTA 360
           PKTGQ+LAHT+LIPNRALKNLIAMWCRQERIPFDVTE+NK  +NGVT NKAALEAMRMTA
Sbjct: 311 PKTGQTLAHTDLIPNRALKNLIAMWCRQERIPFDVTESNKGGINGVTLNKAALEAMRMTA 370

Query: 361 SFLVNKLAT---SSEDANGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQV 420
           SFLVNKLAT   S  DANGVV+ELRVLAKTDSGSRG IA+AGALPLLVR L SDDPTLQV
Sbjct: 371 SFLVNKLATPLDSVNDANGVVYELRVLAKTDSGSRGFIAQAGALPLLVRHLISDDPTLQV 430

Query: 421 NAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 480
           NAVTTVLNLSI EANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR
Sbjct: 431 NAVTTVLNLSILEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 490

Query: 481 RLGRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSL 540
           RLGRK RV+RGLLDLAKDGPI+SKRDALVTILTLAGDRETVGRLIEGGVMETVSH+MNSL
Sbjct: 491 RLGRKTRVIRGLLDLAKDGPISSKRDALVTILTLAGDRETVGRLIEGGVMETVSHVMNSL 550

Query: 541 PEEAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEM 600
           PEEAVTILE+VVRKGGFVAIAS F +IKKLG VLREGSDRARESAAAALVTMCRQGGS+M
Sbjct: 551 PEEAVTILEMVVRKGGFVAIASAFNLIKKLGAVLREGSDRARESAAAALVTMCRQGGSDM 610

Query: 601 VSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNG-VGGDSMTVTSS 655
           V+ELASMAGIERVIWE+MGSGTMRGRRKAASLLRILRRWAAGLD NG VGG  +T+ SS
Sbjct: 611 VTELASMAGIERVIWEMMGSGTMRGRRKAASLLRILRRWAAGLDRNGGVGGGDLTMVSS 669

[Close]

BLAST of MC00g0475 vs. NCBI nr
Match: XP_022966266.1 (U-box domain-containing protein 16-like [Cucurbita maxima])

HSP 1 Score: 1106 bits (2861), Expect = 0.0
Identity = 584/662 (88.22%), Postives = 619/662 (93.50%), Query Frame = 0


Query: 1   RKRRPSAAAFVSPKFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLY 60
           RKRRPSAAAFVSP FSA  LL+SLL LCQEISAMKPLHFLL RYSNS+IRKSRLLE+ L 
Sbjct: 11  RKRRPSAAAFVSPNFSAQALLDSLLCLCQEISAMKPLHFLLNRYSNSMIRKSRLLEVILE 70

Query: 61  DLRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLD 120
           D RR+RIVS SASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQN+S+A+NFHELTLD
Sbjct: 71  DFRRDRIVSLSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNQSIANNFHELTLD 130

Query: 121 LSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIV 180
           LSTLLD+FPVKDAGLTEDVEE+FYLLR+ CSES AFVDPRDEDLR RV  TIDRIRDEIV
Sbjct: 131 LSTLLDVFPVKDAGLTEDVEELFYLLRSHCSESFAFVDPRDEDLRFRVLKTIDRIRDEIV 190

Query: 181 PDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKCVLFG 240
           PD++ELSEIFSR++LRDSSSCREEIENLEDEVQNQ DEKS+SDV A+IG VRYAKCVL+G
Sbjct: 191 PDYTELSEIFSRIELRDSSSCREEIENLEDEVQNQTDEKSRSDVIALIGFVRYAKCVLYG 250

Query: 241 ASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTC 300
           ASTPE GF+RKDSISDLALPADFRCPISLDLM DPVVVATGHTYDRAAITLWIESGHNTC
Sbjct: 251 ASTPESGFQRKDSISDLALPADFRCPISLDLMHDPVVVATGHTYDRAAITLWIESGHNTC 310

Query: 301 PKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTA 360
           PKTGQ+L+HT+LIPNRALKNLIAMWCRQERIPFDVTE+NK  +NGVT NKAALEAMRMTA
Sbjct: 311 PKTGQTLSHTDLIPNRALKNLIAMWCRQERIPFDVTESNKGGINGVTLNKAALEAMRMTA 370

Query: 361 SFLVNKLAT---SSEDANGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQV 420
           SFLVNKLAT   S  DANGVV+ELRVLAKTDSGSRG IA+AGALPLLVR L SD PTLQV
Sbjct: 371 SFLVNKLATPLDSVNDANGVVYELRVLAKTDSGSRGFIAQAGALPLLVRHLISDHPTLQV 430

Query: 421 NAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 480
           NAVTTVLNLSI EANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR
Sbjct: 431 NAVTTVLNLSILEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 490

Query: 481 RLGRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSL 540
           RLGRK RV+RGLLDLAKDGPI+SKRDALVTILTLAGDRETVGRLIEGGVMETVSH+MNSL
Sbjct: 491 RLGRKTRVIRGLLDLAKDGPISSKRDALVTILTLAGDRETVGRLIEGGVMETVSHVMNSL 550

Query: 541 PEEAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEM 600
           PEEAVTILE+VVRKGGFVAIAS F +IKKLG VLREGSDRARESAAAALVTMCRQGGS+M
Sbjct: 551 PEEAVTILEMVVRKGGFVAIASAFNLIKKLGAVLREGSDRARESAAAALVTMCRQGGSDM 610

Query: 601 VSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNGV--GGDSMTVTSS 657
           V+ELASMAGIERVIWE+MGSGTMRGRRKAASLLRILRRWAAGLD NG   GGD   V+SS
Sbjct: 611 VTELASMAGIERVIWEMMGSGTMRGRRKAASLLRILRRWAAGLDRNGGLGGGDLTMVSSS 670

[Close]

BLAST of MC00g0475 vs. NCBI nr
Match: XP_038883276.1 (U-box domain-containing protein 16 [Benincasa hispida])

HSP 1 Score: 1105 bits (2858), Expect = 0.0
Identity = 586/661 (88.65%), Postives = 622/661 (94.10%), Query Frame = 0


Query: 1   RKRRPSAAAFVSPKFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLY 60
           RKRRPSAAAFVSPK SA ILLESLL+L QEIS MKPL FLL RYSNS+IRKSRLLEIFL 
Sbjct: 11  RKRRPSAAAFVSPKLSAPILLESLLSLSQEISTMKPLQFLLNRYSNSMIRKSRLLEIFLQ 70

Query: 61  DLRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLD 120
           DLRRN+I+S   SASLCLEEMYIVLQRIKTLIEDCS+GSKMWLLTQNES+A++FHELTLD
Sbjct: 71  DLRRNQILSLP-SASLCLEEMYIVLQRIKTLIEDCSSGSKMWLLTQNESIANHFHELTLD 130

Query: 121 LSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIV 180
           LSTLLDIFPVKDAGLTEDVEE+FYLLRN CSEST F+DPRDE LR RV   IDRI+DEIV
Sbjct: 131 LSTLLDIFPVKDAGLTEDVEELFYLLRNHCSESTVFLDPRDETLRFRVLKMIDRIKDEIV 190

Query: 181 PDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKCVLFG 240
           PD+SELSEIFS +D+RDSSSCREEIENLEDE+QNQ DEKS+SDV A+IGLVRYAKCVL+G
Sbjct: 191 PDYSELSEIFSMIDIRDSSSCREEIENLEDEIQNQTDEKSRSDVIALIGLVRYAKCVLYG 250

Query: 241 ASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTC 300
           AST E  FRRKDSISDLA+PADFRCPISLDLMQDPVVVATGHTYDRAAI LWIESGHNTC
Sbjct: 251 ASTAEYSFRRKDSISDLAIPADFRCPISLDLMQDPVVVATGHTYDRAAIMLWIESGHNTC 310

Query: 301 PKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTA 360
           PKTGQ+LAHTNLIPNRALKNLIAMWCRQERIPFDVTE+NKE VN VT NKAALEAMRMTA
Sbjct: 311 PKTGQTLAHTNLIPNRALKNLIAMWCRQERIPFDVTESNKEGVNDVTLNKAALEAMRMTA 370

Query: 361 SFLVNKLATSSEDA-NGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQVNA 420
           +FLVNKLATS + + N VV+ELRVLAKTDSGSRG IA+AGALPLLVR L+SD+P LQVNA
Sbjct: 371 TFLVNKLATSVDSSVNDVVYELRVLAKTDSGSRGYIAQAGALPLLVRYLNSDNPILQVNA 430

Query: 421 VTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRRRL 480
           VTTVLNLSI+EANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSS+HSYRRRL
Sbjct: 431 VTTVLNLSIFEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSIHSYRRRL 490

Query: 481 GRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSLPE 540
           GRK RV+RGLLDLAKDGPI+SKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSLPE
Sbjct: 491 GRKTRVIRGLLDLAKDGPISSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSLPE 550

Query: 541 EAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEMVS 600
           EAVTILEVVVRKGGFVAIASGFY+IKKLG+VLREGSDRARESAAAALVTMCRQGGSEMV+
Sbjct: 551 EAVTILEVVVRKGGFVAIASGFYLIKKLGVVLREGSDRARESAAAALVTMCRQGGSEMVT 610

Query: 601 ELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDG-NGVGGDSMTVTSSRIG 659
           ELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLD   G GGDS+TVTSSRIG
Sbjct: 611 ELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDAIGGGGGDSITVTSSRIG 670

[Close]

BLAST of MC00g0475 vs. ExPASy TrEMBL
Match: A0A6J1EB23 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432499 PE=4 SV=1)

HSP 1 Score: 1118 bits (2892), Expect = 0.0
Identity = 590/659 (89.53%), Postives = 620/659 (94.08%), Query Frame = 0


Query: 1   RKRRPSAAAFVSPKFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLY 60
           RKRRPSAAAFVSP FSA  LL+SLL LCQEISAMKPLHFLL RYSNS+IRKSRLLE+ L 
Sbjct: 11  RKRRPSAAAFVSPNFSAQALLDSLLCLCQEISAMKPLHFLLNRYSNSMIRKSRLLEVILE 70

Query: 61  DLRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLD 120
           D RR+RIVS SASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNES+A+NFHELTLD
Sbjct: 71  DFRRDRIVSLSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESIANNFHELTLD 130

Query: 121 LSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIV 180
           LSTLLDIFPVKDAGLTEDVEE+FYLLRN CSES+AFVDPRDEDLR RV  TIDRIRDEIV
Sbjct: 131 LSTLLDIFPVKDAGLTEDVEELFYLLRNHCSESSAFVDPRDEDLRFRVLKTIDRIRDEIV 190

Query: 181 PDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKCVLFG 240
           PD++ELSEIFSR+DLRDSSSCREEIENLEDEVQNQ DEKS+SDV A+IG VRYAKCVL+G
Sbjct: 191 PDYTELSEIFSRIDLRDSSSCREEIENLEDEVQNQTDEKSRSDVIALIGFVRYAKCVLYG 250

Query: 241 ASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTC 300
           ASTPE GF+RKDSISDLALPADFRCPISLDLM DPVVVATGHTYDRAAITLWIESGHNTC
Sbjct: 251 ASTPESGFQRKDSISDLALPADFRCPISLDLMHDPVVVATGHTYDRAAITLWIESGHNTC 310

Query: 301 PKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTA 360
           PKTGQ+LAHT+LIPNRALKNLIAMWCRQERIPFDVTE+NK  +NGVT NKAALEAMRMTA
Sbjct: 311 PKTGQTLAHTDLIPNRALKNLIAMWCRQERIPFDVTESNKGGINGVTLNKAALEAMRMTA 370

Query: 361 SFLVNKLAT---SSEDANGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQV 420
           SFLVNKLAT   S  DANGVV+ELRVLAKTDSGSRG IA+AGALPLLVR L SDDPTLQV
Sbjct: 371 SFLVNKLATPLDSVNDANGVVYELRVLAKTDSGSRGFIAQAGALPLLVRHLISDDPTLQV 430

Query: 421 NAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 480
           NAVTTVLNLSI EANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR
Sbjct: 431 NAVTTVLNLSILEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 490

Query: 481 RLGRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSL 540
           RLGRK RV+RGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSH+MNSL
Sbjct: 491 RLGRKTRVIRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHVMNSL 550

Query: 541 PEEAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEM 600
           PEEAVTILE+VVRKGGFVAIAS F +IKKLG VLREGSDRARESAAAALVTMCRQGGS+M
Sbjct: 551 PEEAVTILEMVVRKGGFVAIASAFNLIKKLGTVLREGSDRARESAAAALVTMCRQGGSDM 610

Query: 601 VSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNG-VGGDSMTVTSS 655
           V+ELASMAGIERVIWE+MGSGTMRGRRKAASLLRILRRWAAGLD NG VGG  +T+ SS
Sbjct: 611 VTELASMAGIERVIWEMMGSGTMRGRRKAASLLRILRRWAAGLDRNGGVGGGDLTMVSS 669

[Close]

BLAST of MC00g0475 vs. ExPASy TrEMBL
Match: A0A6J1HNV9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465978 PE=4 SV=1)

HSP 1 Score: 1106 bits (2861), Expect = 0.0
Identity = 584/662 (88.22%), Postives = 619/662 (93.50%), Query Frame = 0


Query: 1   RKRRPSAAAFVSPKFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLY 60
           RKRRPSAAAFVSP FSA  LL+SLL LCQEISAMKPLHFLL RYSNS+IRKSRLLE+ L 
Sbjct: 11  RKRRPSAAAFVSPNFSAQALLDSLLCLCQEISAMKPLHFLLNRYSNSMIRKSRLLEVILE 70

Query: 61  DLRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLD 120
           D RR+RIVS SASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQN+S+A+NFHELTLD
Sbjct: 71  DFRRDRIVSLSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNQSIANNFHELTLD 130

Query: 121 LSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIV 180
           LSTLLD+FPVKDAGLTEDVEE+FYLLR+ CSES AFVDPRDEDLR RV  TIDRIRDEIV
Sbjct: 131 LSTLLDVFPVKDAGLTEDVEELFYLLRSHCSESFAFVDPRDEDLRFRVLKTIDRIRDEIV 190

Query: 181 PDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKCVLFG 240
           PD++ELSEIFSR++LRDSSSCREEIENLEDEVQNQ DEKS+SDV A+IG VRYAKCVL+G
Sbjct: 191 PDYTELSEIFSRIELRDSSSCREEIENLEDEVQNQTDEKSRSDVIALIGFVRYAKCVLYG 250

Query: 241 ASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTC 300
           ASTPE GF+RKDSISDLALPADFRCPISLDLM DPVVVATGHTYDRAAITLWIESGHNTC
Sbjct: 251 ASTPESGFQRKDSISDLALPADFRCPISLDLMHDPVVVATGHTYDRAAITLWIESGHNTC 310

Query: 301 PKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTA 360
           PKTGQ+L+HT+LIPNRALKNLIAMWCRQERIPFDVTE+NK  +NGVT NKAALEAMRMTA
Sbjct: 311 PKTGQTLSHTDLIPNRALKNLIAMWCRQERIPFDVTESNKGGINGVTLNKAALEAMRMTA 370

Query: 361 SFLVNKLAT---SSEDANGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQV 420
           SFLVNKLAT   S  DANGVV+ELRVLAKTDSGSRG IA+AGALPLLVR L SD PTLQV
Sbjct: 371 SFLVNKLATPLDSVNDANGVVYELRVLAKTDSGSRGFIAQAGALPLLVRHLISDHPTLQV 430

Query: 421 NAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 480
           NAVTTVLNLSI EANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR
Sbjct: 431 NAVTTVLNLSILEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 490

Query: 481 RLGRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSL 540
           RLGRK RV+RGLLDLAKDGPI+SKRDALVTILTLAGDRETVGRLIEGGVMETVSH+MNSL
Sbjct: 491 RLGRKTRVIRGLLDLAKDGPISSKRDALVTILTLAGDRETVGRLIEGGVMETVSHVMNSL 550

Query: 541 PEEAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEM 600
           PEEAVTILE+VVRKGGFVAIAS F +IKKLG VLREGSDRARESAAAALVTMCRQGGS+M
Sbjct: 551 PEEAVTILEMVVRKGGFVAIASAFNLIKKLGAVLREGSDRARESAAAALVTMCRQGGSDM 610

Query: 601 VSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNGV--GGDSMTVTSS 657
           V+ELASMAGIERVIWE+MGSGTMRGRRKAASLLRILRRWAAGLD NG   GGD   V+SS
Sbjct: 611 VTELASMAGIERVIWEMMGSGTMRGRRKAASLLRILRRWAAGLDRNGGLGGGDLTMVSSS 670

[Close]

BLAST of MC00g0475 vs. ExPASy TrEMBL
Match: A0A6J1GCH7 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452957 PE=4 SV=1)

HSP 1 Score: 1097 bits (2836), Expect = 0.0
Identity = 575/661 (86.99%), Postives = 619/661 (93.65%), Query Frame = 0


Query: 1   RKRRPSAAAFVSPKFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLY 60
           RKRRPSAAAFVSPK SA ILLESLL+LC EISA+KPL F+LKRYS S+IRKSRLL IFL 
Sbjct: 11  RKRRPSAAAFVSPKLSAPILLESLLSLCHEISAIKPLQFILKRYSISMIRKSRLLHIFLQ 70

Query: 61  DLRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLD 120
           DLRRN  V  SASA LCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQN+S+A+NFHELTLD
Sbjct: 71  DLRRNPTVLLSASALLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNQSIANNFHELTLD 130

Query: 121 LSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIV 180
           LSTLLDIFPVKDAGLTEDVEE+F+LLRNQCSES AF+DPRDEDLR  V NTIDRI+DEIV
Sbjct: 131 LSTLLDIFPVKDAGLTEDVEELFFLLRNQCSESAAFLDPRDEDLRLSVMNTIDRIKDEIV 190

Query: 181 PDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKCVLFG 240
           PD +ELSEIFS +D+RDSSSCREEIENLEDEVQNQ DEKS+SD+ A+IGLVRYAKCVL+G
Sbjct: 191 PDCAELSEIFSMIDIRDSSSCREEIENLEDEVQNQTDEKSRSDMIALIGLVRYAKCVLYG 250

Query: 241 ASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTC 300
           AST E GFRR DSISDL +PADF+CPI+LDLMQDPVVVATGHTYDRAAITLWIESGHNTC
Sbjct: 251 ASTAESGFRRSDSISDLPVPADFKCPINLDLMQDPVVVATGHTYDRAAITLWIESGHNTC 310

Query: 301 PKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTA 360
           PKTGQ+LAHTNLIPNR LKNLIAMWCRQERIPFDV E+NKERVNGVT NKAALEAMRMTA
Sbjct: 311 PKTGQTLAHTNLIPNRGLKNLIAMWCRQERIPFDVAESNKERVNGVTLNKAALEAMRMTA 370

Query: 361 SFLVNKLATSSEDA-NGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQVNA 420
           SFLV KLATS++ + N VV+ELRVLAKTD GSRG IA+AGA+PLL+R L+SD+PTLQVNA
Sbjct: 371 SFLVKKLATSADSSVNDVVYELRVLAKTDPGSRGFIAQAGAVPLLIRYLNSDNPTLQVNA 430

Query: 421 VTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRRRL 480
           VTTVLNLSI+EANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSS+HSYRRR+
Sbjct: 431 VTTVLNLSIFEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSIHSYRRRM 490

Query: 481 GRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSLPE 540
           GRK+RV+RGLLDLAK+GPINSKRDALVTILTLA DRE VGRLIEGGVME VSHLMNSLPE
Sbjct: 491 GRKSRVIRGLLDLAKNGPINSKRDALVTILTLANDREIVGRLIEGGVMEAVSHLMNSLPE 550

Query: 541 EAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEMVS 600
           EAVTILEVVVRKGGFVAIASGFYVIKKLG VLREGSDRARESAAAALVTMCRQGGSEMV+
Sbjct: 551 EAVTILEVVVRKGGFVAIASGFYVIKKLGSVLREGSDRARESAAAALVTMCRQGGSEMVA 610

Query: 601 ELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNGVGG-DSMTVTSSRIG 659
           ELAS+AGIERVIWELMGSGT RGRRKAASLLRILRRW+AGLDGNG  G +SMT+TSSR+G
Sbjct: 611 ELASIAGIERVIWELMGSGTARGRRKAASLLRILRRWSAGLDGNGGAGVESMTITSSRMG 670

[Close]

BLAST of MC00g0475 vs. ExPASy TrEMBL
Match: A0A6J1IS24 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478185 PE=4 SV=1)

HSP 1 Score: 1095 bits (2832), Expect = 0.0
Identity = 573/661 (86.69%), Postives = 618/661 (93.49%), Query Frame = 0


Query: 1   RKRRPSAAAFVSPKFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLY 60
           RKRRPSAAAFVSPK SA ILLESLL+LC EISA+KPL F+LKRYS S+IRKSRLL IFL 
Sbjct: 11  RKRRPSAAAFVSPKLSAPILLESLLSLCHEISAIKPLQFILKRYSISMIRKSRLLHIFLQ 70

Query: 61  DLRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLD 120
           DLRRN  V  SASA LCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQN+S+A+NFHELTLD
Sbjct: 71  DLRRNPTVLLSASALLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNQSIANNFHELTLD 130

Query: 121 LSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIV 180
           LSTLLDIFPVKDAGLTEDVEE+F+LLRNQCSES AF+DPRDEDLR  V NTIDRI+DEIV
Sbjct: 131 LSTLLDIFPVKDAGLTEDVEELFFLLRNQCSESAAFLDPRDEDLRLSVMNTIDRIKDEIV 190

Query: 181 PDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKCVLFG 240
           PD +ELSEIFS +D+RDSSSCREEIENLEDEVQNQ DEKS+SD+ A+IGLVRYAKCVL+G
Sbjct: 191 PDCAELSEIFSMIDIRDSSSCREEIENLEDEVQNQTDEKSRSDMIALIGLVRYAKCVLYG 250

Query: 241 ASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTC 300
           AST E GFRR DSISDL +PADF+CPI+LDLMQDPVVVATGHTYDRAAITLWIESGHNTC
Sbjct: 251 ASTAESGFRRSDSISDLPVPADFKCPINLDLMQDPVVVATGHTYDRAAITLWIESGHNTC 310

Query: 301 PKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTA 360
           PKTGQ+LAHTNLIPNR LKNLIAMWCRQER+PFDV E+NKERVNGVT NKAALEAMRMTA
Sbjct: 311 PKTGQTLAHTNLIPNRGLKNLIAMWCRQERVPFDVAESNKERVNGVTLNKAALEAMRMTA 370

Query: 361 SFLVNKLATSSE-DANGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQVNA 420
           SFLV KLATS++   N VV+ELRVLAKTD GSRG IA+AGA+PLL+R L+SD+PTLQVNA
Sbjct: 371 SFLVKKLATSADYSVNDVVYELRVLAKTDPGSRGFIAQAGAVPLLIRYLNSDNPTLQVNA 430

Query: 421 VTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRRRL 480
           VTTVLNLSI+EANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSS+HSYRRR+
Sbjct: 431 VTTVLNLSIFEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSIHSYRRRM 490

Query: 481 GRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSLPE 540
           GRK+RV+RGLLDLAK+GPINSKRDALVTILTLA DRE VGRLIEGGVME VSHLM SLPE
Sbjct: 491 GRKSRVIRGLLDLAKNGPINSKRDALVTILTLANDREIVGRLIEGGVMEAVSHLMKSLPE 550

Query: 541 EAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEMVS 600
           EAVTILEVVVRKGGFVAIASGFYVIKKLG VLREGSDRARESAAAALVTMCRQGGSEMV+
Sbjct: 551 EAVTILEVVVRKGGFVAIASGFYVIKKLGSVLREGSDRARESAAAALVTMCRQGGSEMVA 610

Query: 601 ELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNG-VGGDSMTVTSSRIG 659
           ELAS+AGIERV+WELMGSGT RGRRKAASLLRILRRW+AGLDGNG  GG+SMT+TSSR+G
Sbjct: 611 ELASIAGIERVMWELMGSGTARGRRKAASLLRILRRWSAGLDGNGGAGGESMTMTSSRMG 670

[Close]

BLAST of MC00g0475 vs. ExPASy TrEMBL
Match: A0A5D3CE42 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00880 PE=4 SV=1)

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 572/663 (86.27%), Postives = 618/663 (93.21%), Query Frame = 0


Query: 1   RKRRPSAAAFVSPKFSASILLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFLY 60
           RKRRPSAAAFVSPK SA ILL+SLL+L QEIS+ KPL FLL RYS S+IRKS LLEI L+
Sbjct: 11  RKRRPSAAAFVSPKLSAPILLQSLLSLSQEISSTKPLKFLLHRYSISMIRKSLLLEIILH 70

Query: 61  DL-RRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTL 120
           DL RR  I S S SASLCLEEMYIVLQRIKTL+EDCSNGS +WLLTQN+S+A+NFHELTL
Sbjct: 71  DLLRRLPIPSLSPSASLCLEEMYIVLQRIKTLLEDCSNGSNIWLLTQNQSIANNFHELTL 130

Query: 121 DLSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEI 180
           DLSTLLDIFPVKDAGLTEDVEE+FYLLRNQ SES+ F+DPRDE LR RV   IDRI+DEI
Sbjct: 131 DLSTLLDIFPVKDAGLTEDVEELFYLLRNQTSESSVFLDPRDEALRFRVLKMIDRIKDEI 190

Query: 181 VPDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKCVLF 240
           VPDHSEL EIF+ +D+RDSSSCREEIENLEDE+QNQ DEKS+SDV A+IGLVRYAKCVL+
Sbjct: 191 VPDHSELLEIFTMIDIRDSSSCREEIENLEDEIQNQTDEKSRSDVVALIGLVRYAKCVLY 250

Query: 241 GASTP-EPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHN 300
           GAST  E GF+RKDSISDL +PADFRCPISLDLMQDPVVVATGHTYDRAAIT WIESGHN
Sbjct: 251 GASTTAEYGFQRKDSISDLPVPADFRCPISLDLMQDPVVVATGHTYDRAAITFWIESGHN 310

Query: 301 TCPKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRM 360
           TCPKTGQ+LAHTNLIPNRALKNLIAMWCRQERIPFD+TE++KERVN VT NKAALEAMRM
Sbjct: 311 TCPKTGQTLAHTNLIPNRALKNLIAMWCRQERIPFDITESSKERVNDVTLNKAALEAMRM 370

Query: 361 TASFLVNKLATSSEDA-NGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQV 420
           TA+FLVNKLATS + + N VV+ELRVLAKTD GSRG IA+AGALPLLVR L+S++P LQV
Sbjct: 371 TATFLVNKLATSVDSSVNDVVYELRVLAKTDPGSRGYIAQAGALPLLVRYLNSNNPILQV 430

Query: 421 NAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRR 480
           NAVTTVLNLSI+E+NKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSS+HSYRR
Sbjct: 431 NAVTTVLNLSIFESNKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSIHSYRR 490

Query: 481 RLGRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNSL 540
           RLGRK RV+RGLLDLAKDGPI+SKRDALVTILTLAGDRETVGRL+EGGVMETVS+LM+SL
Sbjct: 491 RLGRKTRVIRGLLDLAKDGPISSKRDALVTILTLAGDRETVGRLVEGGVMETVSYLMHSL 550

Query: 541 PEEAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEM 600
           PEEAVTILEVVVRKGGFVAIASGFY+IKKLG+VLREGSDRARESAAAALVTMCRQGGSEM
Sbjct: 551 PEEAVTILEVVVRKGGFVAIASGFYLIKKLGVVLREGSDRARESAAAALVTMCRQGGSEM 610

Query: 601 VSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNG-VGGDSMTVTSSR 659
           V+ELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNG  GGDSMTVTSSR
Sbjct: 611 VTELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNGGAGGDSMTVTSSR 670

[Close]

BLAST of MC00g0475 vs. TAIR 10
Match: AT5G01830.1 (ARM repeat superfamily protein )

HSP 1 Score: 726.5 bits (1874), Expect = 1.9e-209
Identity = 390/664 (58.73%), Postives = 501/664 (75.45%), Query Frame = 0


Query: 1   RKRRP-SAAAFVSPKFSASI-LLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIF 60
           RKRRP    +F SPK S+   L  SL     EIS+M+PL F+L+R S SLIRK ++L   
Sbjct: 12  RKRRPLVVGSFESPKLSSDTKLTRSLFLASHEISSMQPLPFILRRNSLSLIRKVKILASV 71

Query: 61  LYD--LRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHE 120
             +  L R+++V +S SA LC EEM IV+QRIK+LI+DCS  SK+WLL Q + VA NFHE
Sbjct: 72  FDELLLPRSQLVVYSQSAHLCFEEMQIVMQRIKSLIDDCSRVSKLWLLLQIDIVAFNFHE 131

Query: 121 LTLDLSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIR 180
           L  DLST+LDI P+ D  L++D +++  LL  QCS+S  FVD RD  LRR+V++TI  I+
Sbjct: 132 LVTDLSTVLDILPLHDFDLSDDAQDLISLLTKQCSDSVQFVDARDVALRRKVTDTIAGIK 191

Query: 181 DEIVPDHSELSEIFSRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDVSAMIGLVRYAKC 240
            +I PDHS L +IF+ L L DS+S  +EI+ LEDE+Q+QID++SKS  +++IGLVRY+KC
Sbjct: 192 HQISPDHSTLIKIFNDLGLSDSASLTDEIQRLEDEIQDQIDDRSKSAAASLIGLVRYSKC 251

Query: 241 VLFGASTPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESG 300
           VL+G STP P FRR  S+SD  +PADFRCPI+L+LM+DPVVVATG TYDR +I LWI+SG
Sbjct: 252 VLYGPSTPAPDFRRHQSLSDANIPADFRCPITLELMRDPVVVATGQTYDRESIDLWIQSG 311

Query: 301 HNTCPKTGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAM 360
           HNTCPKTGQ L HT+L+PNRALKNLI +WCR ++IPF++  +           K A+E  
Sbjct: 312 HNTCPKTGQVLKHTSLVPNRALKNLIVLWCRDQKIPFELYGDGG---GEPAPCKEAVEFT 371

Query: 361 RMTASFLVNKLATSSEDANGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQ 420
           +M  SFL+ KL+ +  D+NGVV ELR LAK+D+ +R CIA+AGA+P LVR L+++ P+LQ
Sbjct: 372 KMMVSFLIEKLSVA--DSNGVVFELRALAKSDTVARACIAEAGAIPKLVRYLATECPSLQ 431

Query: 421 VNAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYR 480
           +NAVTT+LNLSI E NK+ IMETDGAL GVIEVLRSGATWEAK NAAAT+FSL+ V +YR
Sbjct: 432 INAVTTILNLSILEQNKTRIMETDGALNGVIEVLRSGATWEAKANAAATLFSLAGVSAYR 491

Query: 481 RRLGRKARVVRGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLMNS 540
           RRLGRKARVV GL+DLAK GP +SKRDALV IL L  +RE VGR +E GVM         
Sbjct: 492 RRLGRKARVVSGLVDLAKQGPTSSKRDALVAILNLVAERENVGRFVEAGVMGAAGDAFQE 551

Query: 541 LPEEAVTILEVVVRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSE 600
           LPEEAV ++E VVR+GG +A+++ F +I+ LG V+REG+D  RESAAA LVTMCR+GGSE
Sbjct: 552 LPEEAVAVVEAVVRRGGLMAVSAAFSLIRLLGEVMREGADTTRESAAATLVTMCRKGGSE 611

Query: 601 MVSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLDGNGVGGDSMTV---T 658
           +V+E+A++ GIERVIWE++G+GT RG RKAASL+R LRRWAAG D +    ++ ++   T
Sbjct: 612 LVAEMAAIPGIERVIWEMIGAGTARGGRKAASLMRYLRRWAAG-DTHNTAAETQSIVVPT 669

[Close]

BLAST of MC00g0475 vs. TAIR 10
Match: AT1G29340.1 (plant U-box 17 )

HSP 1 Score: 414.1 bits (1063), Expect = 2.1e-115
Identity = 270/701 (38.52%), Postives = 397/701 (56.63%), Query Frame = 0


Query: 1   RKRRPSAAAFVSP-KFSASILLESLLALCQE-ISAMKPLHFLLKR-YSNSLIRKSRLLEI 60
           R+R PS  AF++P   S   L+++L ++  E +S    + F  +R  + SLIRK  +  +
Sbjct: 12  RRRSPSLEAFLAPVDLSGVALVQTLASISSEVVSCFTSVRFSFQRKNARSLIRKIEIFVV 71

Query: 61  ---FLYD------------LRRNRIVSFSASASLCLEEMYIVLQRIKTLIEDCSNGSKMW 120
              FL D             RR++     ++A LCL+E+Y++L R K L++ C+  SK+W
Sbjct: 72  LFEFLVDSNWGSTTTRTRARRRSKSSVSESTALLCLKELYLLLYRSKILVDYCAQSSKLW 131

Query: 121 LLTQNESVASNFHELTLDLSTLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDE 180
           LL QN S++  FH+L  ++STLLD+ PV D GL++D+ E   LL+ Q  ++  ++D  DE
Sbjct: 132 LLLQNPSISGYFHDLNQEISTLLDVLPVNDLGLSDDIREQIELLQRQSRKARLYIDKNDE 191

Query: 181 DLRRRVSNTIDRIRDEIVPDHSELSEIF-SRLDLRDSSSCREEIENLEDEVQNQID--EK 240
            LR    + +D   +  +P   +L   F  +L +RDS SCR EIE LE+++ N     E 
Sbjct: 192 SLRESFYSFLDGFENGKIPSSVDLRMFFVEKLGIRDSKSCRSEIEFLEEQIVNHDGDLEP 251

Query: 241 SKSDVSAMIGLVRYAKCVLFG-------------ASTPEPGFRRKDSISD--LALPADFR 300
           + S ++  + + RY + +LFG                P  GF  ++ I D  + +P DF 
Sbjct: 252 TGSVINGFVAITRYCRFLLFGFEEDGMEWWIENNPKKPRKGFVAQE-IGDTFITVPKDFV 311

Query: 301 CPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTCPKTGQSLAHTNLIPNRALKNLIAM 360
           CPISLDLM DPV+++TG TYDR +I  WIE GH TCPKTGQ L  + ++PNRALKNLI  
Sbjct: 312 CPISLDLMTDPVIISTGQTYDRNSIARWIEEGHCTCPKTGQMLMDSRIVPNRALKNLIVQ 371

Query: 361 WCRQERIPF--DVTENNKERVNGVTSNKAALEAMRMTASFLVNKLATSSEDANGV-VHEL 420
           WC    I +  + T++  E        KAA+EA + T S L+  LA  S+ A  V   E+
Sbjct: 372 WCTASGISYESEFTDSPNESFASALPTKAAVEANKATVSILIKYLADGSQAAQTVAAREI 431

Query: 421 RVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQVNAVTTVLNLSIYEANKSLIMETDG 480
           R+LAKT   +R  IA+AGA+P L R L+S++   Q N+VT +LNLSIYE NKS IME   
Sbjct: 432 RLLAKTGKENRAYIAEAGAIPHLCRLLTSENAIAQENSVTAMLNLSIYEKNKSRIMEEGD 491

Query: 481 ALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRRRLGRKARVVRGLLDLAKDGPINSK 540
            L  ++ VL SG T EA+ NAAAT+FSLS+VH Y++R+    + V  L  L ++G    K
Sbjct: 492 CLESIVSVLVSGLTVEAQENAAATLFSLSAVHEYKKRIAIVDQCVEALALLLQNGTPRGK 551

Query: 541 RDALVTILTLAGDRETVGRLIE-GGVMETVSHLMN-SLPEEAVTILEVVVRKG-GFVAIA 600
           +DA+  +  L+   +   R+IE GGV   V  L N  + EEA   L ++VR+  G  AI 
Sbjct: 552 KDAVTALYNLSTHPDNCSRMIEGGGVSSLVGALKNEGVAEEAAGALALLVRQSLGAEAIG 611

Query: 601 SGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEMVSELASMAGIERVIWELMGSG 660
                +  L  ++R G+ R +E+A AAL+ +CR GG+ +  ++     I  ++  L+ +G
Sbjct: 612 KEDSAVAGLMGMMRCGTPRGKENAVAALLELCRSGGAAVAEKVLRAPAIAGLLQTLLFTG 671

[Close]

BLAST of MC00g0475 vs. TAIR 10
Match: AT1G60190.1 (ARM repeat superfamily protein )

HSP 1 Score: 285.4 bits (729), Expect = 1.1e-76
Identity = 210/663 (31.67%), Postives = 360/663 (54.30%), Query Frame = 0


Query: 16  SASILLESLLALCQEISAMKPLHFLL-KRYSNSLIRKSRLLEIFLYDLR-RNRIVSFSAS 75
           S + L++SLL L  EI + KP HF   KR     +R  + L IF  +LR + R+ S  A 
Sbjct: 25  SLTTLVDSLLQLAGEILSFKPKHFSTNKRSVKETLRHVQTLVIFFEELRIQIRVGSIPAG 84

Query: 76  AS--LCLEEMYIVLQRIKTLIEDCS-NGSKMWLLTQNESVASNFHELTLDLSTLLDIFPV 135
            S  L L E++++ Q++K L++DC+ +G+K+++L  +  V+++F +LT  +ST LD FPV
Sbjct: 85  RSVILSLSELHVIFQKLKFLLDDCTRDGAKLYMLMNSGQVSAHFRDLTRSISTSLDTFPV 144

Query: 136 KDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIVPDHSELSEIF 195
           +   L  +V E+ YL+  Q  +S A  D  D+     V    +   + I P+  E+  + 
Sbjct: 145 RSVDLPGEVNELIYLVMRQTRKSEARPDRDDKRAIDSVYWFFNLFENRINPNSDEILRVL 204

Query: 196 SRLDLRDSSSCREEIENLEDEVQNQIDEKSKSDV---SAMIGLVRYAKCVLFGASTPEPG 255
             + +R    C +EI+ L +E+   + +KS  ++   S ++G + Y +CV+      +  
Sbjct: 205 DHIGVRKWRDCVKEIDFLREEI--SVGKKSNIEIELLSNLMGFICYCRCVILRGIDVDDE 264

Query: 256 FRRKDSISDLALPA-----DFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTCPK 315
            + K+   DL +       D RCPISL++M DPVV+ +GHTYDR++IT W  SG+ TCPK
Sbjct: 265 EKDKEE-DDLMMVRSLNVDDLRCPISLEIMSDPVVLESGHTYDRSSITKWFASGNITCPK 324

Query: 316 TGQSLAHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTASF 375
           TG++L  T L+ N ++K +I  + +Q  +   + +  K++V+ V  + AA EA ++TA F
Sbjct: 325 TGKTLVSTVLVDNFSVKQVIQSYSKQNGVV--MGQKGKKKVD-VAESLAAEEAGKLTAEF 384

Query: 376 LVNKLATSSEDAN-GVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQVNAVT 435
           L  +L    E+     + E+R+L KT +  R C+ +AG +  L++ L SDDP +Q NA+ 
Sbjct: 385 LAGELIKGDEEEMVKALVEIRILTKTSTFYRSCLVEAGVVESLMKILRSDDPRIQENAMA 444

Query: 436 TVLNLSIYEANKSLIM-ETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRRRLG 495
            ++NLS   A K+ I+ E  G L  ++EVL  GA  E++  AAA +F LSS+  Y R +G
Sbjct: 445 GIMNLSKDIAGKTRIVGEDGGGLRLIVEVLNDGARRESRQYAAAALFYLSSLGDYSRLIG 504

Query: 496 RKARVVRGLLDLAK--DGPINSKRDALVTILTLAGDR-ETVGRLIEGGVMETVSHLMNS- 555
             +  + GL+ + K  D   ++KR+AL+ I +L  ++ +   R++  G++  +  L+ S 
Sbjct: 505 EISDAIPGLVRIVKSCDYGDSAKRNALIAIRSLLMNQPDNHWRILAAGIVPVLLDLVKSE 564

Query: 556 -----LPEEAVTILE----------VVVRKGGFVAIASGFYVIKKLGIVLREGSDRARES 615
                +  +++ IL            V+R+GG          +K LG    E S   ++ 
Sbjct: 565 EISDGVTADSMAILAKMAEYPDGMISVLRRGGLK------LAVKILG--SSEVSPATKQH 624

Query: 616 AAAALVTMCRQGGSEMVSELASMAGIERVIWELMGSGTMRGRRKAASLLRILRRWAAGLD 645
             A L+ +C  GGS++V  LA    I   ++    +G + G +KA++L++++  +     
Sbjct: 625 CVALLLNLCHNGGSDVVGSLAKNPSIMGSLYTASSNGELGGGKKASALIKMIHEFQERKT 673

[Close]

BLAST of MC00g0475 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 261.5 bits (667), Expect = 1.8e-69
Identity = 201/634 (31.70%), Postives = 328/634 (51.74%), Query Frame = 0


Query: 20  LLESLLALCQEISAMKPLHFLLKRYSNSLIRKSRLLEIFL-------YDLRRNRIVSFSA 79
           L+  L+   +EIS        + +    L+R+  LL  F         +L++++I  F  
Sbjct: 12  LMSRLVDSVKEISGFSSSRGFIGKIQGDLVRRITLLSPFFEELIDVNVELKKDQITGF-- 71

Query: 80  SASLCLEEMYIVLQRIKTLIEDCSNGSKMWLLTQNESVASNFHELTLDLSTLLDIFPVKD 139
                 E M I L     L    + GSK++ L   +S+   F ++T+++   L   P + 
Sbjct: 72  ------EAMRIALDSSLELFRSVNGGSKLFQLFDRDSLVEKFRDMTVEIEAALSQIPYEK 131

Query: 140 AGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIRDEIVPDHSELSEIFSR 199
             ++E+V E   LL  Q   +      R E+   ++S+ +    + + PD   L  +   
Sbjct: 132 IEVSEEVREQVQLLHFQFKRA----KERWEESDLQLSHDLAMAENVMDPDPIILKRLSQE 191

Query: 200 LDLRDSSSCREEIENLED---EVQNQIDEKSKSDVSAMIGLVRYAKCVLFGASTPEP--G 259
           L L      ++E   + +         D+  +   S +  LV +   V   +S P+P  G
Sbjct: 192 LQLTTIDELKKESHAIHEYFLSYDGDPDDCFERMSSLLKNLVDF---VTMESSDPDPSTG 251

Query: 260 FRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTYDRAAITLWIESGHNTCPKTGQSL 319
            R         +P  FRCPISL+LM+DPV+V+TG TY+R++I  W+++GH TCPK+ ++L
Sbjct: 252 SRIVSRHRSPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETL 311

Query: 320 AHTNLIPNRALKNLIAMWCRQERIPFDVTENNKERVNGVTSNKAALEAMRMTASFLVNKL 379
            H  L PN  LK+LIA+WC    I  ++ +N            ++ +  R     L+ KL
Sbjct: 312 LHAGLTPNYVLKSLIALWCESNGI--ELPQNQGSCRTTKIGGSSSSDCDRTFVLSLLEKL 371

Query: 380 AT-SSEDANGVVHELRVLAKTDSGSRGCIAKAGALPLLVRCLSSDDPTLQVNAVTTVLNL 439
           A  ++E       ELR+LAK +  +R CIA+AGA+PLLV  LSS DP  Q ++VT +LNL
Sbjct: 372 ANGTTEQQRAAAGELRLLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVTALLNL 431

Query: 440 SIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNAAATIFSLSSVHSYRRRLGRKARVV 499
           SI E NK  I++  GA+  ++EVL++G + EA+ NAAAT+FSLS +   +  +G  A  +
Sbjct: 432 SINEGNKGAIVDA-GAITDIVEVLKNG-SMEARENAAATLFSLSVIDENKVAIG-AAGAI 491

Query: 500 RGLLDLAKDGPINSKRDALVTILTLAGDRETVGRLIEGGVMETVSHLM----NSLPEEAV 559
           + L+ L ++G    K+DA   I  L   +    R ++GG+++ ++ L+      + +EA+
Sbjct: 492 QALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDEAL 551

Query: 560 TILEVV-VRKGGFVAIASGFYVIKKLGIVLREGSDRARESAAAALVTMCRQGGSEMVSEL 619
            IL ++   + G  AIA     I  L  ++R GS R RE+AAA L  +C  G  E ++ +
Sbjct: 552 AILAILSTNQEGKTAIAEA-ESIPVLVEIIRTGSPRNRENAAAILWYLC-IGNIERLN-V 611

Query: 620 ASMAGIERVIWELMGSGTMRGRRKAASLLRILRR 636
           A   G +  + EL  +GT R +RKAASLL ++++
Sbjct: 612 AREVGADVALKELTENGTDRAKRKAASLLELIQQ 622

[Close]

BLAST of MC00g0475 vs. TAIR 10
Match: AT1G10560.1 (plant U-box 18 )

HSP 1 Score: 256.5 bits (654), Expect = 5.7e-68
Identity = 193/665 (29.02%), Postives = 340/665 (51.13%), Query Frame = 0


Query: 11  VSPKFSASI--LLESLLALCQEISAMKPLHFLLKRYS--NSLIRKSRLLEIF---LYDLR 70
           V P  S SI  LL+SL+ L  +I   K  HF   + S   +L R   LL +F      +R
Sbjct: 18  VEPSESISIVTLLDSLIQLAGDILTFKSKHFSTNKQSFRETLRRIQNLLVVFEEIRIRIR 77

Query: 71  RNRIVSFSASASLCLEEMYIVLQRIKTLIEDCS-NGSKMWLLTQNESVASNFHELTLDLS 130
            +R     ++A+  L+E+++  Q++K L+EDC+ +G+++ ++  ++ V+ +   LT  +S
Sbjct: 78  NSRRYFHDSAAASSLKEIHVGFQKLKFLLEDCTRDGARLCMMMNSDQVSDHLRVLTRSIS 137

Query: 131 TLLDIFPVKDAGLTEDVEEIFYLLRNQCSESTAFVDPRDEDLRRRVSNTIDRIR----DE 190
           T L  FPV    LT +V E+  L+  Q  +    V P   D  +R  ++I+RI     + 
Sbjct: 138 TSLSAFPVASVDLTTEVNELIDLVVRQARKYG--VQPETND--KRAVSSINRILALFVNR 197

Query: 191 IVPDHSELSEIFSRLDLRDSSSCREEIENLEDEVQ-NQIDEKSKSD------VSAMIGLV 250
           +VPD  E++ I   + +R    C +EI  L +E+   ++DEK K        +S+++G +
Sbjct: 198 VVPDPDEINRILDHVGIRKWGDCVKEINFLGEEIDAERLDEKKKKSSDQVELLSSLMGFI 257

Query: 251 RYAKCVLFGA-------STPEPGFRRKDSISDLALPADFRCPISLDLMQDPVVVATGHTY 310
            Y +C++ G        +  E G ++   +       D  CPISL++M DPVV+ TGHTY
Sbjct: 258 CYCRCIILGRIERDDHHNHHEDGIKKDHDLIRGLKVEDLLCPISLEIMTDPVVIETGHTY 317

Query: 311 DRAAITLWIESGHNTCPKTGQSLAHTNLIPNRALKNLIAMWCRQERIPF-DVTENNKERV 370
           DR++IT W  SG+ TCP TG+ L  T L+ N +++ +I   C+   I    ++   K   
Sbjct: 318 DRSSITKWFGSGNITCPITGKILTSTELVDNVSVRQVIRKHCKTNGIVLAGISRRRKSHD 377

Query: 371 NGVTSNKAALEAMRMTASFLVNKLATSSED-ANGVVHELRVLAKTDSGSRGCIAKAGALP 430
           + V  + AA  A ++ A FL ++L    E+     V E+RV  KT S +R C+ KAGA+ 
Sbjct: 378 DVVPESLAAKGAGKLIAKFLTSELINGGEEMIYRAVREIRVQTKTSSFNRSCLVKAGAVT 437

Query: 431 LLVRCLSSDDPTLQVNAVTTVLNLSIYEANKSLIMETDGALIGVIEVLRSGATWEAKGNA 490
            L++ LSS D  +Q NA+  +LNLS +   KS I   +G  I ++E+L  GA  E +  +
Sbjct: 438 PLLKLLSSVDIRIQENAMAGILNLSKHVTGKSKI-AGEGLKI-LVEILNEGAKTETRLYS 497

Query: 491 AATIFSLSSVHSYRRRLGRKARVVRGLLDLAK--DGPINSKRDALVTILTLAGDRETVGR 550
           A+ +F LSSV  Y R +G     + GL+++ K  D   ++KR AL+ ++ L    +   R
Sbjct: 498 ASALFYLSSVEDYSRLIGENPDAIPGLMNIVKGDDYGDSAKRSALLAVMGLLMQSDNHWR 557

Query: 551 LIEGGVMETVSHLMNS-------LPEEAVTILEVVVRKGGFVAI--ASGFYVIKKLGIVL 610
           ++  G +  +  L+ S         +   T+ ++     G + +    G  +  K+    
Sbjct: 558 VLAAGAVPILLDLLRSGEISGGLTADCLATLAKLAEYPDGTIGVIRRGGLKLAVKILSSS 617

Query: 611 REGSDRARESAAAALVTMCRQGGSEMVSELASMAGIERVIWELMGSGTMRGRRKAASLLR 637
            +     ++     ++ +C  GG ++V  L   + +   ++ ++ +G   G +KA++L+R
Sbjct: 618 EDSPVAVKQHCVGLILNLCLNGGRDVVGVLVKNSLVMGSLYTVLSNGEYGGSKKASALIR 676

The following BLAST results are available for this feature:

BLAST of MC00g0475 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZW3	2.7e-208	58.73	U-box domain-containing protein 16 OS=Arabidopsis thaliana OX=3702 GN=PUB16 PE=2...	[more]
Q9C7R6	3.0e-114	38.52	U-box domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=PUB17 PE=2...	[more]
Q6EUK7	2.0e-97	34.59	U-box domain-containing protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=PU...	[more]
E4NKF8	2.0e-89	32.65	U-box domain-containing protein 1 OS=Medicago truncatula OX=3880 GN=PUB1 PE=1 SV...	[more]
O80742	1.6e-75	31.67	U-box domain-containing protein 19 OS=Arabidopsis thaliana OX=3702 GN=PUB19 PE=2...	[more]

BLAST of MC00g0475 vs. NCBI nr


Match Name	Identity	Description
XP_022925177.1	89.53	U-box domain-containing protein 16-like [Cucurbita moschata]	[more]
KAG6595558.1	89.23	U-box domain-containing protein 16, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_023521732.1	89.23	U-box domain-containing protein 16-like [Cucurbita pepo subsp. pepo]	[more]
XP_022966266.1	88.22	U-box domain-containing protein 16-like [Cucurbita maxima]	[more]
XP_038883276.1	88.65	U-box domain-containing protein 16 [Benincasa hispida]	[more]

BLAST of MC00g0475 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1EB23	89.53	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432499...	[more]
A0A6J1HNV9	88.22	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111465978 P...	[more]
A0A6J1GCH7	86.99	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452957...	[more]
A0A6J1IS24	86.69	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478185 P...	[more]
A0A5D3CE42	86.27	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of MC00g0475 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01830.1	1.9e-209	58.73	ARM repeat superfamily protein	[more]
AT1G29340.1	2.1e-115	38.52	plant U-box 17	[more]
AT1G60190.1	1.1e-76	31.67	ARM repeat superfamily protein	[more]
AT3G54850.1	1.8e-69	31.70	plant U-box 14	[more]
AT1G10560.1	5.7e-68	29.02	plant U-box 18	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 198..222
None	No IPR available	PANTHER	PTHR23315:SF63	U-BOX DOMAIN-CONTAINING PROTEIN 16	coord: 4..648
None	No IPR available	PANTHER	PTHR23315	U BOX DOMAIN-CONTAINING	coord: 4..648
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 263..305 e-value: 2.05372E-26 score: 99.6154
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 253..331
IPR000225	Armadillo	SMART	SM00185	arm_5	coord: 388..428 e-value: 0.0026 score: 27.0
IPR000225	Armadillo	PFAM	PF00514	Arm	coord: 391..427 e-value: 7.4E-8 score: 32.2
IPR000225	Armadillo	PROSITE	PS50176	ARM_REPEAT	coord: 399..441 score: 11.5674
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 262..325 e-value: 1.0E-31 score: 121.3
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 260..330 e-value: 1.3E-19 score: 70.1
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 258..332 score: 39.285519
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 246..340 e-value: 1.1E-29 score: 104.4
IPR036537	Adaptor protein Cbl, N-terminal domain superfamily	GENE3D	1.20.930.20		coord: 20..130 e-value: 1.7E-6 score: 29.9
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 365..635 e-value: 2.0E-24 score: 88.2
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 371..633

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC00g0475.1	MC00g0475.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007166	cell surface receptor signaling pathway
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity