MC05g0257 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0257
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	heavy metal-associated isoprenylated plant protein 4
Location	MC05: 1862051 .. 1865897 (+)
RNA-Seq Expression	MC05g0257
Synteny	MC05g0257

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGCAGAGAAGGAGCTGGCAAAGGCAAGAGTGGAGGTGGCTCATGAGGTAAGATTGGCAAGAGAAGCTGAAGCCGCAATGGATCTTCATGTGGCCAAAGCTGGAGAAAAGGCTGAGAGAGAAATGGCAAAGTATGCTCATACTCACCCCACTGCCTTTGTGGCTCCCGGCGGCACCACCAACTTGGCCGCCGCTCCCGTCGATGTCACCATGGTCGGGCCACCCGGCACCGTCGACATGACCGGAACCGTTCCCGTTAAGAAGATGCTATAAATTGATCATATGCTTGTTTTGTTTTGTTTCTGTCTTTCGCAACTTCTACCGTATCTTCAAATGCTGTGTAGTAGAATGTAAGAATTTGTATGGTTTCGTTTTTAATATTATTGATGGATCGTTGAGGTTTCTCCTTGTACTATTATCGTTTTTTTCTTCGAAAGATACTGGAACCATTTCATTAAAATATGTGAGAAACATATCTATAAAAAAAATGGAAAAGTTATATAAAAGTACGTGCTCTGGTATAACGAAATGAAAGAGAAGATATTATAATTACAATGGAAGATCCAGAGAGAGTTATTATCGTATTTCAAGGACGTTCATGTATTAACACAGTAATTATTTTGATAACCCATTTTATTTTTATTATTATTATTATTATTATTTTTTCTAAAGATAAAAGGGAAAAAGTTCGATTCAATTTGGCACAAGAAAGACAAACTTTGGGTGGTGTAAAATGCAAGACACTATTCCAAGCAGTAGTTGAACCAACTCTCAGCAATTCTTACCAAAACGATGTCGTGTTGAGAAGAAATGACTAGAAGGGTCTCTTTTCTACCTATTCTCTTGTGTTATATATGAACATGAAAGATTTCTTGTGCACATTTTTTGTTGTGGAGTTTTTTGGTGCAAAAATGGCAGAGAAAGAGAAGAAGATTGAAGTCATCACTGCCATTTACAAAGTAAACCTTCATTGCCATCAATGTTGGAGAGAAATCAAAAAACCTCTTGCAAGAACACAAGGTAAAATTAATTTATCATTTTCTTACTTGCTTTTTTCTCATTCGTTTTCCCTCATTTTCTTGGCAACCAAACACGTTTAGAATAGAAATTTTTGCAAACCTCCTCGTTCTACCATGACAAAAGGATGACTTTTGAAAATATTTTTCAAAACTTTTTTAGTATTTCTTTACATAATCAAAAGGTAATCTAAAAATGGGACGCTTGCAAAAAAGAAATAATAACAAAGTTTGAAAGTCCAACCTATGAAAAAAAAAATGCAATACTCTAATATATAGCCTTAATGAACATGAAGCCCATATATATATATGTATATATATTCGATACACCATTTATATATTTGAAAATTTGAAAACGTACTATTAGAATAATTAGAAAATCGAAAAAAATCATATTTTAAACCTAAAAGTTGTTGCTATATTTGCAAATATGACAAATATTTTAGCTACTTATTATAAATTCTCTTTCAAAATTCTAAAAATACTTAAAAACGACTTAATTAGTATTTATCACTAAAAAAGGCTTATAAAAGAAGTATCAATGAAATAAATTTTTTTTTTTAAATTGCAAACCGTCCATGAATTAAGAACTCGTGATAGAATGCTCTAAATCTTCACACCTCACGGGTCAATAGTCATTTAAGACTGCCATTTCTTTTATATTTAGAATTTTGGTGGTGTGGTTGTATGTGTGTATATATATACTGTGTGATGGAATTATTGATCCTTTAACCTAATGTTTTTTTTTTAATTTTTTTAATATTGATTATTAGGGGTTCAAAGTGTCGAAGTTGAAATGGAGAAAAATGAAATTAAGGTGAAGGGTTCAAATTTGGATGTGCTGAAAATTCATAAGCAAATAGAAAAGTTGAGCAAGAAAAAGGTTGAGTTGATATCTCCCAAAGTGCAGCCCAACGAAAAAGAAACCCCCAAACCCACAGACAACAAACCCAAACAAATCCTTGTGAGTTTAATATTTTTTATTAACATTAATTTATATATATCCATTTTGTTCCGTCTGTAATTTCAAAAAGTCAATTTTAGTTCGTTAATTTTAAAAAAGTGACTATTTTTAATTTTTATCTTATTTTTTTCATCATAATTATATGGTTGTAATTTCAAAAAGTCTATTTTAATTTGTAAACTTTTAAAAAATAACTATTCCTTTTAATTTTTTCCTCGATCATAATTTTATCATCATACTTCACTCAACTTCTTAAGATTTAGTCATAAATTATGTGTTAGTTTCTATCAAGTATTCAATTATACAAATAGAGACTAAATCGATCGTTTTTGAAGGGTACGTAGTCTGAAATGGACGTTACTAACATGAGTTTAATTGATGTATGTCTCTAACCAAAAGATCATGAGTTCGATTCTCCCATGGTAAAAAAAAATATTATTAAATTTTTTTTTCTTTTTAAATGGAAGAATATTAATCACATTACAAGTATAGGGGTGCAAATTCCAACCTCAACCTTTCTTTTTTCTATTTTTTATTTATTCTTTTCATTTGGCAGATCCATCGAACAATAACAGCCAAGGTTCACTTGCACTGCGCCAAATGTGAGCAAGACCTCAAGAACAAGCTGCTAAAGCATAAAGGTTCGCTGGCAAATCCTTTTTCATTTTATATTCTTAAATACTTTATTTTAGTTATGATCTTGTATAAATTATATTTTAATTTGTACAATTAAAAAGGCTACAATATTAAAAGTATACTAAATTTGAAAGCAAAAAATATTGTAACTTCTAAATTTGCTTTGATTTAATTTTAAACTCTCAAAAGATTGTGTTTTAGTTCTTAAACTTGGTCTCTCTCAGGGACCAAAATTTATGTTAAATTATTATCAAATAATTAATCAACCGGATTAAAATAGTTCGTTATGCAAAATTTTCAAACATCAGAACAAAATATTTAAAATTAATTTATAGAATAATACTTGGTAAATTAACTACTACTTTCATGTTAAATCCCCAAAAATTACATTATGAAAAATCAATGTATATTCTAGTTTCAACAATTTATTCATTCTGATGCAAAATCTGTCAGTAATTTGACATGCCTTATCTGATAATTATAAAGAACATGTTTCTAATAATTACGAAAATGGCAATTCAATAATTATTGAAAAATATAATAAACATATAAAAAAATTACTATTTCTCCAATTTCACTACTTTTGTAAATTGTGAAAATAGCTACAAAATTAGTCAACCTCTAAAACTGGAGCCATTTGAAAATAAGAAGCTATATATTTGCAGGTATATATAGTGTGAAAACTGATATCAAAGCACAAACCCTAACGATTGAAGGAACCATAGAACCAGAGAAATTCAGATCCTACCTGAAGAAGAAGCTGCACAAACACATAGACATTACCGCCGACAAGAAATCGGTAGATTCGTCGAAGCCATCGACGGAGAAGAAGAATGAAAGTACTGAGAAAGAGAAGCCGAAAGAAAAATCTTCATCTAAGACGACGACCACTAAAATTGCAGAGATTCAAATCAAACAAAGCGACAATAATGTTAATAACAACAGTAATGTTCCATATTTCATTCACTATGTCTATGCTCCACAGTTGTTCAGTGATGAAAATCCAAACGCATGTCGAGTAATGTAAGTAACGGTTGATTTTTTACGATAAAAATTTCATATTTCAACTAATTAAATAAGAGAAATTTGATATTGAAAGTGTGCTTATTTTTTTATTTTTAACAAATCACGTTAGTTCCTTTAAAATCTATAGCCTTTTTGAGATCTTCATAGGCTATTGAAACTCATTCTCATTTGTAATTTAAATGTAAGCAATTTAAACTAAATCTTG

mRNA sequence

CAGGCAGAGAAGGAGCTGGCAAAGGCAAGAGTGGAGGTGGCTCATGAGGTAAGATTGGCAAGAGAAGCTGAAGCCGCAATGGATCTTCATGTGGCCAAAGCTGGAGAAAAGGCTGAGAGAGAAATGGCAAAGTATGCTCATACTCACCCCACTGCCTTTGTGGCTCCCGGCGGCACCACCAACTTGGCCGCCGCTCCCGTCGATGTCACCATGGTCGGGCCACCCGGCACCGTCGACATGACCGGAACCGTTCCCGTTAAGAAGATGCTAAAATTGATCATATGCTTGTTTTGTTTTGTTTCTGTCTTTCGCAACTTCTACCGTATCTTCAAATGCTGTGTAGTAGAATATTTCTTGTGCACATTTTTTGTTGTGGAGTTTTTTGGTGCAAAAATGGCAGAGAAAGAGAAGAAGATTGAAGTCATCACTGCCATTTACAAAGTAAACCTTCATTGCCATCAATGTTGGAGAGAAATCAAAAAACCTCTTGCAAGAACACAAGGGGTTCAAAGTGTCGAAGTTGAAATGGAGAAAAATGAAATTAAGGTGAAGGGTTCAAATTTGGATGTGCTGAAAATTCATAAGCAAATAGAAAAGTTGAGCAAGAAAAAGGTTGAGTTGATATCTCCCAAAGTGCAGCCCAACGAAAAAGAAACCCCCAAACCCACAGACAACAAACCCAAACAAATCCTTATCCATCGAACAATAACAGCCAAGGTTCACTTGCACTGCGCCAAATGTGAGCAAGACCTCAAGAACAAGCTGCTAAAGCATAAAGGTATATATAGTGTGAAAACTGATATCAAAGCACAAACCCTAACGATTGAAGGAACCATAGAACCAGAGAAATTCAGATCCTACCTGAAGAAGAAGCTGCACAAACACATAGACATTACCGCCGACAAGAAATCGGTAGATTCGTCGAAGCCATCGACGGAGAAGAAGAATGAAAGTACTGAGAAAGAGAAGCCGAAAGAAAAATCTTCATCTAAGACGACGACCACTAAAATTGCAGAGATTCAAATCAAACAAAGCGACAATAATGTTAATAACAACAGTAATGTTCCATATTTCATTCACTATGTCTATGCTCCACAGTTGTTCAGTGATGAAAATCCAAACGCATGTCGAGTAATGTAAGTAACGGTTGATTTTTTACGATAAAAATTTCATATTTCAACTAATTAAATAAGAGAAATTTGATATTGAAAGTGTGCTTATTTTTTTATTTTTAACAAATCACGTTAGTTCCTTTAAAATCTATAGCCTTTTTGAGATCTTCATAGGCTATTGAAACTCATTCTCATTTGTAATTTAAATGTAAGCAATTTAAACTAAATCTTG

Coding sequence (CDS)

CAGGCAGAGAAGGAGCTGGCAAAGGCAAGAGTGGAGGTGGCTCATGAGGTAAGATTGGCAAGAGAAGCTGAAGCCGCAATGGATCTTCATGTGGCCAAAGCTGGAGAAAAGGCTGAGAGAGAAATGGCAAAGTATGCTCATACTCACCCCACTGCCTTTGTGGCTCCCGGCGGCACCACCAACTTGGCCGCCGCTCCCGTCGATGTCACCATGGTCGGGCCACCCGGCACCGTCGACATGACCGGAACCGTTCCCGTTAAGAAGATGCTAAAATTGATCATATGCTTGTTTTGTTTTGTTTCTGTCTTTCGCAACTTCTACCGTATCTTCAAATGCTGTGTAGTAGAATATTTCTTGTGCACATTTTTTGTTGTGGAGTTTTTTGGTGCAAAAATGGCAGAGAAAGAGAAGAAGATTGAAGTCATCACTGCCATTTACAAAGTAAACCTTCATTGCCATCAATGTTGGAGAGAAATCAAAAAACCTCTTGCAAGAACACAAGGGGTTCAAAGTGTCGAAGTTGAAATGGAGAAAAATGAAATTAAGGTGAAGGGTTCAAATTTGGATGTGCTGAAAATTCATAAGCAAATAGAAAAGTTGAGCAAGAAAAAGGTTGAGTTGATATCTCCCAAAGTGCAGCCCAACGAAAAAGAAACCCCCAAACCCACAGACAACAAACCCAAACAAATCCTTATCCATCGAACAATAACAGCCAAGGTTCACTTGCACTGCGCCAAATGTGAGCAAGACCTCAAGAACAAGCTGCTAAAGCATAAAGGTATATATAGTGTGAAAACTGATATCAAAGCACAAACCCTAACGATTGAAGGAACCATAGAACCAGAGAAATTCAGATCCTACCTGAAGAAGAAGCTGCACAAACACATAGACATTACCGCCGACAAGAAATCGGTAGATTCGTCGAAGCCATCGACGGAGAAGAAGAATGAAAGTACTGAGAAAGAGAAGCCGAAAGAAAAATCTTCATCTAAGACGACGACCACTAAAATTGCAGAGATTCAAATCAAACAAAGCGACAATAATGTTAATAACAACAGTAATGTTCCATATTTCATTCACTATGTCTATGCTCCACAGTTGTTCAGTGATGAAAATCCAAACGCATGTCGAGTAATGTAA

Protein sequence

QAEKELAKARVEVAHEVRLAREAEAAMDLHVAKAGEKAEREMAKYAHTHPTAFVAPGGTTNLAAAPVDVTMVGPPGTVDMTGTVPVKKMLKLIICLFCFVSVFRNFYRIFKCCVVEYFLCTFFVVEFFGAKMAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKIHKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDLKNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPSTEKKNESTEKEKPKEKSSSKTTTTKIAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDENPNACRVM

Homology

[Close]

BLAST of MC05g0257 vs. ExPASy Swiss-Prot
Match: Q9SHQ8 (Heavy metal-associated isoprenylated plant protein 4 OS=Arabidopsis thaliana OX=3702 GN=HIPP04 PE=3 SV=2)

HSP 1 Score: 233.4 bits (594), Expect = 4.2e-60
Identity = 139/287 (48.43%), Postives = 184/287 (64.11%), Query Frame = 0


Query: 129 GAKMAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNL 188
           G +  +KEK  E+ITA+YKV+LHC +C  +IKKPL R QGVQ+V+ ++EKNEIKVKG  +
Sbjct: 2   GEEEEKKEKGDEIITAVYKVHLHCRKCACDIKKPLLRFQGVQNVDFDLEKNEIKVKG-KI 61

Query: 189 DVLKIHKQIEKLSKKKVELISPKVQPNEKETPKPT-----DNKPKQIL--IHRTITAKVH 248
           +V+KIHKQIEK SKKKVELISPK    +K T   T     + K  +I   + RT   KVH
Sbjct: 62  EVVKIHKQIEKWSKKKVELISPKPSEVKKTTTTTTTTSVVEKKTTEIKKDVIRTTVLKVH 121

Query: 249 LHCAKCEQDLKNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITAD 308
           +HCA+C++DL++KLLKHK I+ VKTD KAQTLT++GTIE  K  +Y+KKK+HKH +I + 
Sbjct: 122 IHCAQCDKDLQHKLLKHKAIHIVKTDTKAQTLTVQGTIESAKLLAYIKKKVHKHAEIISS 181

Query: 309 ---------------------KKSVDSSKPSTEKKNESTEKEKPKEKS--------SSKT 368
                                KK  D  K   EKK E   K+K  EK         ++KT
Sbjct: 182 KTEEEKKKEEEDKKKKEEEDKKKKEDEKKKEEEKKKEEENKKKEGEKKKEEVKVEVTTKT 241

Query: 369 TTTKIAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDENPNACRVM 380
            T  +   +I + +   + + N+PYF+HYVYAPQLFSDENPNACR++
Sbjct: 242 ITQVVEYKEIVKVEGQKDKDGNIPYFVHYVYAPQLFSDENPNACRIV 287

[Close]

BLAST of MC05g0257 vs. ExPASy Swiss-Prot
Match: Q9C5D3 (Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=3702 GN=HIPP07 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 8.5e-21
Identity = 76/280 (27.14%), Postives = 131/280 (46.79%), Query Frame = 0


Query: 145 IYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKIHKQIEKLSKKK 204
           + KV +HC  C R++++ L   +GV+ V  + +  ++ VKG   D LK+  ++++ + ++
Sbjct: 76  VLKVYMHCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQ 135

Query: 205 VELISPKVQPNEKETPKPTDNKP----KQI--LIHRTITAKVHLHCAKCEQDLKNKLLKH 264
           V+L+SP   P      K  ++KP    K++   +  T+  KVH+HC  C  ++K ++++ 
Sbjct: 136 VQLLSPIPPPPPPPEKKAEEDKPIVEEKKVEPPVVVTVVLKVHMHCEACATEIKKRIMRM 195

Query: 265 KGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDI----------------TADK 324
           KG+ S ++D+K+  +T++G  EP+K   Y+ K+  KH  I                 A+ 
Sbjct: 196 KGVESAESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAIMKIDPPPPPPPEEAAAAAEG 255

Query: 325 KSVDSSKPSTEKKNESTEKEKPKEKSSSKTTTTKIAEIQIKQSDNNVNNNSNV------- 380
           +  +  K   E K E  + +K K     K       E +   +         V       
Sbjct: 256 EKKEEEKGEGESKGEEGKDDKAKTDEEKKEGDGGKGEGEAADNGGGEEEGKVVEVRKIEN 315

[Close]

BLAST of MC05g0257 vs. ExPASy Swiss-Prot
Match: Q9M8T7 (Heavy metal-associated isoprenylated plant protein 8 OS=Arabidopsis thaliana OX=3702 GN=HIPP08 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 3.6e-19
Identity = 69/248 (27.82%), Postives = 117/248 (47.18%), Query Frame = 0


Query: 134 EKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKI 193
           + +KK +    + KV +HC  C  ++   L    GV+ ++ E+  N++ V G   D LKI
Sbjct: 28  KSDKKNQCKEIVLKVYMHCEGCASQVSHCLRGYDGVEHIKTEIGDNKVVVSGKFDDPLKI 87

Query: 194 HKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDLKN 253
            ++++K   +  E+ISPK  P  K+  K    K +     +T   ++++HC  C  ++K 
Sbjct: 88  LRRVQKKFSRNAEMISPKHNP--KQDQKEPQQKKESAPEIKTAILRMNMHCEGCVHEIKR 147

Query: 254 KLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITAD--KKSVDSSKPS 313
            + K KGI SV+ D    T+ + G ++P K    +KKKL KH ++ +   +K  D++K +
Sbjct: 148 GIEKIKGIQSVEPDRSKSTVVVRGVMDPPKLVEKIKKKLGKHAELLSQITEKGKDNNKKN 207

Query: 314 TEKKNESTEKEKPKEKSSSKTTTTKIAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDE 373
             KK E                           SD N   +    Y   + Y  Q+FSDE
Sbjct: 208 NNKKEE---------------------------SDGNKIFSYPPQYSSQHAYPSQIFSDE 246

Query: 374 NPNACRVM 380
           N ++C +M
Sbjct: 268 NVHSCSIM 246

[Close]

BLAST of MC05g0257 vs. ExPASy Swiss-Prot
Match: Q9ZUV1 (Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=3702 GN=HIPP01 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.0e-18
Identity = 60/192 (31.25%), Postives = 107/192 (55.73%), Query Frame = 0


Query: 127 FFGAKMAEKEKKIEVITA----IYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIK 186
           + G    EK+KK    T+    + K++ HC  C   I +   R +GV++V  + + N++ 
Sbjct: 10  YHGDVEEEKKKKQNNTTSPVHVVLKIDFHCDGCIARIVRLSRRLEGVETVRADPDSNKLT 69

Query: 187 VKGSNLDVLKIHKQIEKLSKKKVELISPKVQPNEKE-TPKPTDNKPKQILIHRTITAKVH 246
           + G  +D +KI ++++K SKKKVELISPK + + KE   K  ++K + ++   T+  KV+
Sbjct: 70  LIGFIMDPVKIAEKLQKKSKKKVELISPKPKKDTKENNEKKANDKTQTVVAVTTVVLKVN 129

Query: 247 LHCAKCEQDLKNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITAD 306
             C  C + ++  +   KG+Y VK D + +T+T+ GT++ +     LK+KL K + +  +
Sbjct: 130 CSCDGCIKRIQKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKTVQVVPE 189

Query: 307 KKSVDSSKPSTE 314
           KK     K + E
Sbjct: 190 KKKKKKDKDNAE 201

[Close]

BLAST of MC05g0257 vs. ExPASy Swiss-Prot
Match: Q9FLU5 (Heavy metal-associated isoprenylated plant protein 9 OS=Arabidopsis thaliana OX=3702 GN=HIPP09 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 2.3e-13
Identity = 72/261 (27.59%), Postives = 121/261 (46.36%), Query Frame = 0


Query: 145 IYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKIHKQIEKLSKKK 204
           I  V+LHC  C ++I++ + + +GV+ V ++M +N++ +KG  LD   +  +I+K +K+ 
Sbjct: 59  ILYVDLHCVGCAKKIERSILKIRGVEEVVMDMNENQVTIKGV-LDPQAVCNKIKKKTKRM 118

Query: 205 VELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDLKNKLLKHKGIYSV 264
            +++SP      +  P    ++    L   T+   V++HC  C   LK K+LK +G+ + 
Sbjct: 119 AKVLSPLPAAEGEPLPPIITSQVSGGL--TTVELSVNMHCQACADQLKKKILKMRGVQTT 178

Query: 265 KTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDIT--ADKKSVDSSKPSTEKKNESTE-K 324
            T+     + + GT++ EK   Y+ ++  K   I    D +    +    EKK ES E  
Sbjct: 179 VTEHTTGKVIVTGTMDAEKLVDYVYRRTKKQARIVPQPDPEPEAPAAAQEEKKEESGEGN 238

Query: 325 EKPKEKSSSKTTTTK--------------IAEIQIKQSDNNVNNNS------------NV 377
           EKP E    K    K               A  + ++ DN +   +              
Sbjct: 239 EKPPETGEEKEEEKKKEGEENGEEGGGEEAAATEEERRDNEMTAMAQEEGMKRMMYYYQP 298

[Close]

BLAST of MC05g0257 vs. NCBI nr
Match: XP_022147087.1 (heavy metal-associated isoprenylated plant protein 4 [Momordica charantia])

HSP 1 Score: 471 bits (1211), Expect = 1.10e-164
Identity = 247/248 (99.60%), Postives = 247/248 (99.60%), Query Frame = 0


Query: 132 MAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVL 191
           MAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVL
Sbjct: 1   MAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVL 60

Query: 192 KIHKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDL 251
           K HKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDL
Sbjct: 61  KXHKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDL 120

Query: 252 KNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPS 311
           KNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPS
Sbjct: 121 KNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPS 180

Query: 312 TEKKNESTEKEKPKEKSSSKTTTTKIAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDE 371
           TEKKNESTEKEKPKEKSSSKTTTTKIAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDE
Sbjct: 181 TEKKNESTEKEKPKEKSSSKTTTTKIAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDE 240

Query: 372 NPNACRVM 379
           NPNACRVM
Sbjct: 241 NPNACRVM 248

[Close]

BLAST of MC05g0257 vs. NCBI nr
Match: XP_022980379.1 (heavy metal-associated isoprenylated plant protein 4 [Cucurbita maxima])

HSP 1 Score: 365 bits (938), Expect = 3.13e-123
Identity = 195/249 (78.31%), Postives = 215/249 (86.35%), Query Frame = 0


Query: 137 KKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKIHKQ 196
           +K E ITAIYKVNLHC QCWREIK+PL RTQGV +VEV+MEKNEI+VKGSNLDVLKIHKQ
Sbjct: 3   EKAEAITAIYKVNLHCQQCWREIKRPLVRTQGVHNVEVDMEKNEIRVKGSNLDVLKIHKQ 62

Query: 197 IEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDLKNKLL 256
           IEKLSKK+V+LISPKV+P EKETPKPTDNKPK  LIHRTITAKVHLHCAKCE+DLKN LL
Sbjct: 63  IEKLSKKRVDLISPKVKPKEKETPKPTDNKPKPSLIHRTITAKVHLHCAKCEKDLKNNLL 122

Query: 257 KHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPSTEKKN 316
           KHKGIYSVKTD+KAQTLTIEGTIEPEKF+SYLKKKL KH DIT    + DSSKP  +KK 
Sbjct: 123 KHKGIYSVKTDMKAQTLTIEGTIEPEKFKSYLKKKLQKHADITT---AADSSKPPEKKKE 182

Query: 317 ESTEKEKPKEKSSSKTTTTK------IAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSD 376
            S +KEKPKEKSSS+TTTT       +AEIQ K ++NN NN   VPYF+HYVYAPQLFSD
Sbjct: 183 SSEQKEKPKEKSSSETTTTTTTTTKIVAEIQTKDNENNKNN---VPYFVHYVYAPQLFSD 242

Query: 377 ENPNACRVM 379
           ENPNAC+VM
Sbjct: 243 ENPNACQVM 245

[Close]

BLAST of MC05g0257 vs. NCBI nr
Match: XP_023528992.1 (heavy metal-associated isoprenylated plant protein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 362 bits (930), Expect = 4.78e-122
Identity = 194/247 (78.54%), Postives = 213/247 (86.23%), Query Frame = 0


Query: 137 KKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKIHKQ 196
           +K E ITAIYKVNLHC QCWREIK+PL +TQGV +VEVEMEKNEI+VKGSNLDVLKIHKQ
Sbjct: 3   EKAEAITAIYKVNLHCQQCWREIKRPLLKTQGVHNVEVEMEKNEIRVKGSNLDVLKIHKQ 62

Query: 197 IEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDLKNKLL 256
           IEKLSKK+V+LISPKV+P EKETPKP DNKPK  LIHRTITAKVHLHCAKCE+DLKN LL
Sbjct: 63  IEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIHRTITAKVHLHCAKCEKDLKNNLL 122

Query: 257 KHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPSTEKKN 316
           KHKGIYSVKTD+KAQTLT+EGTIEPEKF+SYLKKKL KH DIT    +VDSSKP  +KK 
Sbjct: 123 KHKGIYSVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITT---AVDSSKPPEKKKE 182

Query: 317 ESTEKEKPKEKSSSKTTTTK----IAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDEN 376
            S +KEKPKEKSS +TTTT     +AEIQ K +DNN NN    PYFIHYVYAPQLFSDEN
Sbjct: 183 SSEQKEKPKEKSSLETTTTTTTKIVAEIQTKDNDNNKNN---APYFIHYVYAPQLFSDEN 242

Query: 377 PNACRVM 379
           PNAC+VM
Sbjct: 243 PNACQVM 243

[Close]

BLAST of MC05g0257 vs. NCBI nr
Match: KAG7018991.1 (Heavy metal-associated isoprenylated plant protein 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 362 bits (929), Expect = 6.77e-122
Identity = 193/247 (78.14%), Postives = 214/247 (86.64%), Query Frame = 0


Query: 137 KKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKIHKQ 196
           +K E ITAIYKVNLHC QCWREIK+PLARTQGV +VEV+MEKNEI+VKGSNLDVLKIHKQ
Sbjct: 3   EKAEAITAIYKVNLHCQQCWREIKRPLARTQGVHNVEVDMEKNEIRVKGSNLDVLKIHKQ 62

Query: 197 IEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDLKNKLL 256
           IEKLSKK+V+LISPKV+P EKETPKP DNKPK  LI RTITAKVHLHCAKCE+DLKN LL
Sbjct: 63  IEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRTITAKVHLHCAKCEKDLKNNLL 122

Query: 257 KHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPSTEKKN 316
           KHKGIY+VKTD+KAQTLT+EGTIEPEKF+SYLKKKL KH DIT    +VDSSKP  +KK 
Sbjct: 123 KHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITT---AVDSSKPPEKKKE 182

Query: 317 ESTEKEKPKEKSSSKTTTTK----IAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDEN 376
            S +KEKPKEKSSS+TTTT     +AEIQ K +DNN NN    PYF+HYVYAPQLFSDEN
Sbjct: 183 SSEQKEKPKEKSSSETTTTTTTKIVAEIQTKDTDNNKNN---APYFVHYVYAPQLFSDEN 242

Query: 377 PNACRVM 379
           PNAC+VM
Sbjct: 243 PNACQVM 243

[Close]

BLAST of MC05g0257 vs. NCBI nr
Match: XP_022924650.1 (heavy metal-associated isoprenylated plant protein 4 [Cucurbita moschata])

HSP 1 Score: 362 bits (928), Expect = 9.61e-122
Identity = 192/247 (77.73%), Postives = 214/247 (86.64%), Query Frame = 0


Query: 137 KKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKIHKQ 196
           +K E ITAIYKVNLHC QCWREIK+PLARTQG+ +VEV+MEKNEI+VKGSNLDVLKIHKQ
Sbjct: 3   EKAEAITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQ 62

Query: 197 IEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDLKNKLL 256
           IEKLSKK+V+LISPKV+P EKETPKP DNKPK  LI RTITAKVHLHCAKCE+DLKN LL
Sbjct: 63  IEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRTITAKVHLHCAKCEKDLKNNLL 122

Query: 257 KHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPSTEKKN 316
           KHKGIY+VKTD+KAQTLT+EGTIEPEKF+SYLKKKL KH DIT    +VDSSKP  +KK 
Sbjct: 123 KHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITT---AVDSSKPPEKKKE 182

Query: 317 ESTEKEKPKEKSSSKTTTTK----IAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDEN 376
            S +KEKPKEKSSS+TTTT     +AEIQ K +DNN NN    PYF+HYVYAPQLFSDEN
Sbjct: 183 SSEQKEKPKEKSSSETTTTTTTKIVAEIQTKDTDNNKNN---APYFVHYVYAPQLFSDEN 242

Query: 377 PNACRVM 379
           PNAC+VM
Sbjct: 243 PNACQVM 243

[Close]

BLAST of MC05g0257 vs. ExPASy TrEMBL
Match: A0A6J1D1E3 (heavy metal-associated isoprenylated plant protein 4 OS=Momordica charantia OX=3673 GN=LOC111016109 PE=3 SV=1)

HSP 1 Score: 471 bits (1211), Expect = 5.31e-165
Identity = 247/248 (99.60%), Postives = 247/248 (99.60%), Query Frame = 0


Query: 132 MAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVL 191
           MAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVL
Sbjct: 1   MAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVL 60

Query: 192 KIHKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDL 251
           K HKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDL
Sbjct: 61  KXHKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDL 120

Query: 252 KNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPS 311
           KNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPS
Sbjct: 121 KNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPS 180

Query: 312 TEKKNESTEKEKPKEKSSSKTTTTKIAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDE 371
           TEKKNESTEKEKPKEKSSSKTTTTKIAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDE
Sbjct: 181 TEKKNESTEKEKPKEKSSSKTTTTKIAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDE 240

Query: 372 NPNACRVM 379
           NPNACRVM
Sbjct: 241 NPNACRVM 248

[Close]

BLAST of MC05g0257 vs. ExPASy TrEMBL
Match: A0A6J1IZ45 (heavy metal-associated isoprenylated plant protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111479765 PE=3 SV=1)

HSP 1 Score: 365 bits (938), Expect = 1.51e-123
Identity = 195/249 (78.31%), Postives = 215/249 (86.35%), Query Frame = 0


Query: 137 KKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKIHKQ 196
           +K E ITAIYKVNLHC QCWREIK+PL RTQGV +VEV+MEKNEI+VKGSNLDVLKIHKQ
Sbjct: 3   EKAEAITAIYKVNLHCQQCWREIKRPLVRTQGVHNVEVDMEKNEIRVKGSNLDVLKIHKQ 62

Query: 197 IEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDLKNKLL 256
           IEKLSKK+V+LISPKV+P EKETPKPTDNKPK  LIHRTITAKVHLHCAKCE+DLKN LL
Sbjct: 63  IEKLSKKRVDLISPKVKPKEKETPKPTDNKPKPSLIHRTITAKVHLHCAKCEKDLKNNLL 122

Query: 257 KHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPSTEKKN 316
           KHKGIYSVKTD+KAQTLTIEGTIEPEKF+SYLKKKL KH DIT    + DSSKP  +KK 
Sbjct: 123 KHKGIYSVKTDMKAQTLTIEGTIEPEKFKSYLKKKLQKHADITT---AADSSKPPEKKKE 182

Query: 317 ESTEKEKPKEKSSSKTTTTK------IAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSD 376
            S +KEKPKEKSSS+TTTT       +AEIQ K ++NN NN   VPYF+HYVYAPQLFSD
Sbjct: 183 SSEQKEKPKEKSSSETTTTTTTTTKIVAEIQTKDNENNKNN---VPYFVHYVYAPQLFSD 242

Query: 377 ENPNACRVM 379
           ENPNAC+VM
Sbjct: 243 ENPNACQVM 245

[Close]

BLAST of MC05g0257 vs. ExPASy TrEMBL
Match: A0A6J1EFL8 (heavy metal-associated isoprenylated plant protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111432082 PE=3 SV=1)

HSP 1 Score: 362 bits (928), Expect = 4.65e-122
Identity = 192/247 (77.73%), Postives = 214/247 (86.64%), Query Frame = 0


Query: 137 KKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKIHKQ 196
           +K E ITAIYKVNLHC QCWREIK+PLARTQG+ +VEV+MEKNEI+VKGSNLDVLKIHKQ
Sbjct: 3   EKAEAITAIYKVNLHCQQCWREIKRPLARTQGIHNVEVDMEKNEIRVKGSNLDVLKIHKQ 62

Query: 197 IEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDLKNKLL 256
           IEKLSKK+V+LISPKV+P EKETPKP DNKPK  LI RTITAKVHLHCAKCE+DLKN LL
Sbjct: 63  IEKLSKKRVDLISPKVKPKEKETPKPNDNKPKPSLIQRTITAKVHLHCAKCEKDLKNNLL 122

Query: 257 KHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPSTEKKN 316
           KHKGIY+VKTD+KAQTLT+EGTIEPEKF+SYLKKKL KH DIT    +VDSSKP  +KK 
Sbjct: 123 KHKGIYNVKTDMKAQTLTMEGTIEPEKFKSYLKKKLQKHADITT---AVDSSKPPEKKKE 182

Query: 317 ESTEKEKPKEKSSSKTTTTK----IAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDEN 376
            S +KEKPKEKSSS+TTTT     +AEIQ K +DNN NN    PYF+HYVYAPQLFSDEN
Sbjct: 183 SSEQKEKPKEKSSSETTTTTTTKIVAEIQTKDTDNNKNN---APYFVHYVYAPQLFSDEN 242

Query: 377 PNACRVM 379
           PNAC+VM
Sbjct: 243 PNACQVM 243

[Close]

BLAST of MC05g0257 vs. ExPASy TrEMBL
Match: A0A1S3AVV8 (heavy metal-associated isoprenylated plant protein 3 OS=Cucumis melo OX=3656 GN=LOC103483396 PE=3 SV=1)

HSP 1 Score: 350 bits (898), Expect = 2.91e-117
Identity = 193/262 (73.66%), Postives = 217/262 (82.82%), Query Frame = 0


Query: 132 MAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVL 191
           M EKEK   +ITAIYK+NLHCHQCWR+IKKPL+ TQGVQ+VEV+MEKNEI+VKGSNLDVL
Sbjct: 1   MGEKEKVEGIITAIYKLNLHCHQCWRDIKKPLSITQGVQNVEVDMEKNEIRVKGSNLDVL 60

Query: 192 KIHKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRTITAKVHLHCAKCEQDL 251
           KI KQIEKLSKKKVELISPKV+P +K+ PKPT +KPK  +++RTITAKVHLHC KCEQDL
Sbjct: 61  KIQKQIEKLSKKKVELISPKVKPKDKDPPKPTADKPKPTIVNRTITAKVHLHCPKCEQDL 120

Query: 252 KNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITADKKSVDSSKPS 311
           KNKLLKHKGIYSVKTDIKAQTLT+EG+IE EKF+SYLK KL KH+DIT D KS D S  S
Sbjct: 121 KNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKHVDITVDTKSTDYSSKS 180

Query: 312 T----EKKNEST--EKEKPKEKSSSKTTTTK--------IAEIQIKQSDNNVNNNSNVPY 371
           T    EKK ES   +KEKPKEK+SS TTTT         +AEIQ K+++N    N NVPY
Sbjct: 181 TAVASEKKKESNTDQKEKPKEKASSDTTTTTTTDKTTIVVAEIQSKENNNK---NDNVPY 240

Query: 372 FIHYVYAPQLFSDENPNACRVM 379
           FIHYVYAPQLFSDENPNACRVM
Sbjct: 241 FIHYVYAPQLFSDENPNACRVM 259

[Close]

BLAST of MC05g0257 vs. ExPASy TrEMBL
Match: A0A0A0L4G4 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G127060 PE=3 SV=1)

HSP 1 Score: 281 bits (720), Expect = 6.53e-91
Identity = 161/218 (73.85%), Postives = 184/218 (84.40%), Query Frame = 0


Query: 176 MEKNEIKVKGSNLDVLKIHKQIEKLSKKKVELISPKVQPNEKETPKPTDNKPKQILIHRT 235
           MEKNEI+VKGSNLDVLKI KQIEKLSKKKVELISPKV+P EK+ PKP D+KPK  +++R 
Sbjct: 1   MEKNEIRVKGSNLDVLKIQKQIEKLSKKKVELISPKVKPKEKDPPKPIDDKPKPTIVNRI 60

Query: 236 ITAKVHLHCAKCEQDLKNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKH 295
           ITAKVHLHC KCEQDLKNKLLKHKGIYSVKTDIKAQTLT+EG+IE EKF+SYLK KL KH
Sbjct: 61  ITAKVHLHCPKCEQDLKNKLLKHKGIYSVKTDIKAQTLTMEGSIEAEKFKSYLKNKLQKH 120

Query: 296 IDITADKKSVDSSKPS---TEKKNEST--EKEKPKEKSSSKTTTTK-------IAEIQIK 355
           +DIT D KS DSSK +   +EKK ES   +KEKPKEK+SS+TT T        +AEIQ K
Sbjct: 121 VDITVDTKSTDSSKSTAVASEKKKESNTDKKEKPKEKASSETTITTTDKKTIVVAEIQSK 180

Query: 356 QSDNN-VNN-NSNVPYFIHYVYAPQLFSDENPNACRVM 379
           ++++N +NN N+NVPYFIHYVYAPQLFSDENPNACRVM
Sbjct: 181 ENNSNDINNKNNNVPYFIHYVYAPQLFSDENPNACRVM 218

[Close]

BLAST of MC05g0257 vs. TAIR 10
Match: AT1G29000.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 3.0e-61
Identity = 139/287 (48.43%), Postives = 184/287 (64.11%), Query Frame = 0


Query: 129 GAKMAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNL 188
           G +  +KEK  E+ITA+YKV+LHC +C  +IKKPL R QGVQ+V+ ++EKNEIKVKG  +
Sbjct: 2   GEEEEKKEKGDEIITAVYKVHLHCRKCACDIKKPLLRFQGVQNVDFDLEKNEIKVKG-KI 61

Query: 189 DVLKIHKQIEKLSKKKVELISPKVQPNEKETPKPT-----DNKPKQIL--IHRTITAKVH 248
           +V+KIHKQIEK SKKKVELISPK    +K T   T     + K  +I   + RT   KVH
Sbjct: 62  EVVKIHKQIEKWSKKKVELISPKPSEVKKTTTTTTTTSVVEKKTTEIKKDVIRTTVLKVH 121

Query: 249 LHCAKCEQDLKNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITAD 308
           +HCA+C++DL++KLLKHK I+ VKTD KAQTLT++GTIE  K  +Y+KKK+HKH +I + 
Sbjct: 122 IHCAQCDKDLQHKLLKHKAIHIVKTDTKAQTLTVQGTIESAKLLAYIKKKVHKHAEIISS 181

Query: 309 ---------------------KKSVDSSKPSTEKKNESTEKEKPKEKS--------SSKT 368
                                KK  D  K   EKK E   K+K  EK         ++KT
Sbjct: 182 KTEEEKKKEEEDKKKKEEEDKKKKEDEKKKEEEKKKEEENKKKEGEKKKEEVKVEVTTKT 241

Query: 369 TTTKIAEIQIKQSDNNVNNNSNVPYFIHYVYAPQLFSDENPNACRVM 380
            T  +   +I + +   + + N+PYF+HYVYAPQLFSDENPNACR++
Sbjct: 242 ITQVVEYKEIVKVEGQKDKDGNIPYFVHYVYAPQLFSDENPNACRIV 287

[Close]

BLAST of MC05g0257 vs. TAIR 10
Match: AT5G50740.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 120.9 bits (302), Expect = 2.2e-27
Identity = 83/272 (30.51%), Postives = 142/272 (52.21%), Query Frame = 0


Query: 131 KMAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDV 190
           K  E+EKK E    + K+ +HC  C ++I + L   +GV+ V  + + +++ VKG   D 
Sbjct: 16  KKPEEEKKKEPQEIVLKIFMHCEGCAKKIHRCLKGFEGVEDVTTDCKTSKVVVKGEKADP 75

Query: 191 LKIHKQIEKLSKKKVELISPKVQP---------NEKETPKPTDNKPKQILIHRTITAKVH 250
           LK+ +++++ S ++VELISP  +P          EKE PKP + K + +    T+  +VH
Sbjct: 76  LKVLQRLQRKSHRQVELISPIPEPKPVSDEPEKKEKEKPKPEEKKEEVV----TVVLRVH 135

Query: 251 LHCAKCEQDLKNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITAD 310
           +HC  C  +++ ++++ KG+ SV+ D KA  ++++G   PEK   ++ K++ KH  +   
Sbjct: 136 MHCEACAMEIQKRIMRMKGVESVEPDFKASQVSVKGVFTPEKLVEFIYKRIGKHAAVVKQ 195

Query: 311 ---KKSVDSSKPSTEKKNESTEKEKPKEKSSSKTT--------TTKIAEIQIKQSDNNVN 370
               K  +  K + +K  +  E+ +PKE   +K              AE   K  D   N
Sbjct: 196 DPPPKPPEKEKETKDKDEKKKEEGQPKEGKEAKENGGGGGAKGDGAAAEEGNKVVDLKKN 255

Query: 371 NNSNVP--YFIH-YVYAPQLFSDENPNACRVM 380
                P  Y +  + Y PQ+FSDENPNAC ++
Sbjct: 256 EYQYQPPRYPVEMFAYPPQIFSDENPNACTII 283

[Close]

BLAST of MC05g0257 vs. TAIR 10
Match: AT5G50740.3 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 120.9 bits (302), Expect = 2.2e-27
Identity = 83/272 (30.51%), Postives = 142/272 (52.21%), Query Frame = 0


Query: 131 KMAEKEKKIEVITAIYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDV 190
           K  E+EKK E    + K+ +HC  C ++I + L   +GV+ V  + + +++ VKG   D 
Sbjct: 23  KKPEEEKKKEPQEIVLKIFMHCEGCAKKIHRCLKGFEGVEDVTTDCKTSKVVVKGEKADP 82

Query: 191 LKIHKQIEKLSKKKVELISPKVQP---------NEKETPKPTDNKPKQILIHRTITAKVH 250
           LK+ +++++ S ++VELISP  +P          EKE PKP + K + +    T+  +VH
Sbjct: 83  LKVLQRLQRKSHRQVELISPIPEPKPVSDEPEKKEKEKPKPEEKKEEVV----TVVLRVH 142

Query: 251 LHCAKCEQDLKNKLLKHKGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDITAD 310
           +HC  C  +++ ++++ KG+ SV+ D KA  ++++G   PEK   ++ K++ KH  +   
Sbjct: 143 MHCEACAMEIQKRIMRMKGVESVEPDFKASQVSVKGVFTPEKLVEFIYKRIGKHAAVVKQ 202

Query: 311 ---KKSVDSSKPSTEKKNESTEKEKPKEKSSSKTT--------TTKIAEIQIKQSDNNVN 370
               K  +  K + +K  +  E+ +PKE   +K              AE   K  D   N
Sbjct: 203 DPPPKPPEKEKETKDKDEKKKEEGQPKEGKEAKENGGGGGAKGDGAAAEEGNKVVDLKKN 262

Query: 371 NNSNVP--YFIH-YVYAPQLFSDENPNACRVM 380
                P  Y +  + Y PQ+FSDENPNAC ++
Sbjct: 263 EYQYQPPRYPVEMFAYPPQIFSDENPNACTII 290

[Close]

BLAST of MC05g0257 vs. TAIR 10
Match: AT5G63530.1 (farnesylated protein 3 )

HSP 1 Score: 102.8 bits (255), Expect = 6.1e-22
Identity = 76/280 (27.14%), Postives = 131/280 (46.79%), Query Frame = 0


Query: 145 IYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKIHKQIEKLSKKK 204
           + KV +HC  C R++++ L   +GV+ V  + +  ++ VKG   D LK+  ++++ + ++
Sbjct: 76  VLKVYMHCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQ 135

Query: 205 VELISPKVQPNEKETPKPTDNKP----KQI--LIHRTITAKVHLHCAKCEQDLKNKLLKH 264
           V+L+SP   P      K  ++KP    K++   +  T+  KVH+HC  C  ++K ++++ 
Sbjct: 136 VQLLSPIPPPPPPPEKKAEEDKPIVEEKKVEPPVVVTVVLKVHMHCEACATEIKKRIMRM 195

Query: 265 KGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDI----------------TADK 324
           KG+ S ++D+K+  +T++G  EP+K   Y+ K+  KH  I                 A+ 
Sbjct: 196 KGVESAESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAIMKIDPPPPPPPEEAAAAAEG 255

Query: 325 KSVDSSKPSTEKKNESTEKEKPKEKSSSKTTTTKIAEIQIKQSDNNVNNNSNV------- 380
           +  +  K   E K E  + +K K     K       E +   +         V       
Sbjct: 256 EKKEEEKGEGESKGEEGKDDKAKTDEEKKEGDGGKGEGEAADNGGGEEEGKVVEVRKIEN 315

[Close]

BLAST of MC05g0257 vs. TAIR 10
Match: AT5G63530.2 (farnesylated protein 3 )

HSP 1 Score: 102.8 bits (255), Expect = 6.1e-22
Identity = 76/280 (27.14%), Postives = 131/280 (46.79%), Query Frame = 0


Query: 145 IYKVNLHCHQCWREIKKPLARTQGVQSVEVEMEKNEIKVKGSNLDVLKIHKQIEKLSKKK 204
           + KV +HC  C R++++ L   +GV+ V  + +  ++ VKG   D LK+  ++++ + ++
Sbjct: 61  VLKVYMHCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQ 120

Query: 205 VELISPKVQPNEKETPKPTDNKP----KQI--LIHRTITAKVHLHCAKCEQDLKNKLLKH 264
           V+L+SP   P      K  ++KP    K++   +  T+  KVH+HC  C  ++K ++++ 
Sbjct: 121 VQLLSPIPPPPPPPEKKAEEDKPIVEEKKVEPPVVVTVVLKVHMHCEACATEIKKRIMRM 180

Query: 265 KGIYSVKTDIKAQTLTIEGTIEPEKFRSYLKKKLHKHIDI----------------TADK 324
           KG+ S ++D+K+  +T++G  EP+K   Y+ K+  KH  I                 A+ 
Sbjct: 181 KGVESAESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAIMKIDPPPPPPPEEAAAAAEG 240

Query: 325 KSVDSSKPSTEKKNESTEKEKPKEKSSSKTTTTKIAEIQIKQSDNNVNNNSNV------- 380
           +  +  K   E K E  + +K K     K       E +   +         V       
Sbjct: 241 EKKEEEKGEGESKGEEGKDDKAKTDEEKKEGDGGKGEGEAADNGGGEEEGKVVEVRKIEN 300

The following BLAST results are available for this feature:

BLAST of MC05g0257 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SHQ8	4.2e-60	48.43	Heavy metal-associated isoprenylated plant protein 4 OS=Arabidopsis thaliana OX=...	[more]
Q9C5D3	8.5e-21	27.14	Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=...	[more]
Q9M8T7	3.6e-19	27.82	Heavy metal-associated isoprenylated plant protein 8 OS=Arabidopsis thaliana OX=...	[more]
Q9ZUV1	1.0e-18	31.25	Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=...	[more]
Q9FLU5	2.3e-13	27.59	Heavy metal-associated isoprenylated plant protein 9 OS=Arabidopsis thaliana OX=...	[more]

BLAST of MC05g0257 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147087.1	1.10e-164	99.60	heavy metal-associated isoprenylated plant protein 4 [Momordica charantia]	[more]
XP_022980379.1	3.13e-123	78.31	heavy metal-associated isoprenylated plant protein 4 [Cucurbita maxima]	[more]
XP_023528992.1	4.78e-122	78.54	heavy metal-associated isoprenylated plant protein 4 [Cucurbita pepo subsp. pepo...	[more]
KAG7018991.1	6.77e-122	78.14	Heavy metal-associated isoprenylated plant protein 4, partial [Cucurbita argyros...	[more]
XP_022924650.1	9.61e-122	77.73	heavy metal-associated isoprenylated plant protein 4 [Cucurbita moschata]	[more]

BLAST of MC05g0257 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D1E3	5.31e-165	99.60	heavy metal-associated isoprenylated plant protein 4 OS=Momordica charantia OX=3...	[more]
A0A6J1IZ45	1.51e-123	78.31	heavy metal-associated isoprenylated plant protein 4 OS=Cucurbita maxima OX=3661...	[more]
A0A6J1EFL8	4.65e-122	77.73	heavy metal-associated isoprenylated plant protein 4 OS=Cucurbita moschata OX=36...	[more]
A0A1S3AVV8	2.91e-117	73.66	heavy metal-associated isoprenylated plant protein 3 OS=Cucumis melo OX=3656 GN=...	[more]
A0A0A0L4G4	6.53e-91	73.85	HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G127060 PE=3 SV...	[more]

BLAST of MC05g0257 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G29000.1	3.0e-61	48.43	Heavy metal transport/detoxification superfamily protein	[more]
AT5G50740.1	2.2e-27	30.51	Heavy metal transport/detoxification superfamily protein	[more]
AT5G50740.3	2.2e-27	30.51	Heavy metal transport/detoxification superfamily protein	[more]
AT5G63530.1	6.1e-22	27.14	farnesylated protein 3	[more]
AT5G63530.2	6.1e-22	27.14	farnesylated protein 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.70.100		coord: 233..296 e-value: 1.2E-10 score: 43.7 coord: 139..205 e-value: 4.5E-12 score: 48.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 301..332
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 301..334
None	No IPR available	PANTHER	PTHR46195:SF12	HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 4	coord: 132..379
IPR005513	Late embryogenesis abundant protein, LEA_1 subgroup	PFAM	PF03760	LEA_1	coord: 2..41 e-value: 2.1E-5 score: 25.1
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 240..294 e-value: 1.5E-13 score: 50.9 coord: 147..203 e-value: 2.5E-9 score: 37.4
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 142..206 score: 9.296249
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 150..203 e-value: 3.39761E-5 score: 39.5113
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 241..280 e-value: 5.13397E-4 score: 36.0445
IPR044577	Heavy metal-associated isoprenylated plant protein 4/7/8/17/18/19	PANTHER	PTHR46195	HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 7	coord: 132..379
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 233..290
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 144..203

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0257.1	MC05g0257.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009793	embryo development ending in seed dormancy
molecular_function	GO:0046872	metal ion binding