MC05g0231 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC05g0231
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Malic enzyme
Location	MC05: 1645347 .. 1645484 (+)
RNA-Seq Expression	MC05g0231
Synteny	MC05g0231

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGAGGAGTATGCAGCAATTGTATTACACAGCAACTGTAGGTCTCGTTTGCCAGAATTATGGTGGCTTGTTTAGAAGACCAAGGGGAATGTATTTCAGTGCAGCCGATCGTGGAGAAATGATGTCTATGGTTTATAAT

mRNA sequence

TTGAGGAGTATGCAGCAATTGTATTACACAGCAACTGTAGGTCTCGTTTGCCAGAATTATGGTGGCTTGTTTAGAAGACCAAGGGGAATGTATTTCAGTGCAGCCGATCGTGGAGAAATGATGTCTATGGTTTATAAT

Coding sequence (CDS)

TTGAGGAGTATGCAGCAATTGTATTACACAGCAACTGTAGGTCTCGTTTGCCAGAATTATGGTGGCTTGTTTAGAAGACCAAGGGGAATGTATTTCAGTGCAGCCGATCGTGGAGAAATGATGTCTATGGTTTATAAT

Protein sequence

LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN

Homology

[Close]

BLAST of MC05g0231 vs. ExPASy Swiss-Prot
Match: Q9SIU0 (NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME1 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 2.7e-14
Identity = 35/40 (87.50%), Postives = 36/40 (90.00%), Query Frame = 0


Query: 7   LYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 47
           + YT TVGLVCQNY GLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 141 IVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYN 180

[Close]

BLAST of MC05g0231 vs. ExPASy Swiss-Prot
Match: Q8L7K9 (NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME2 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 8.0e-14
Identity = 32/46 (69.57%), Postives = 38/46 (82.61%), Query Frame = 0


Query: 1   LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 47
           ++    + YT TVGLVCQNY GL+RRPRGMYFSA D+GEMMSM+YN
Sbjct: 128 IKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYN 173

[Close]

BLAST of MC05g0231 vs. ExPASy Swiss-Prot
Match: P37225 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.0e-13
Identity = 32/46 (69.57%), Postives = 38/46 (82.61%), Query Frame = 0


Query: 1   LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 47
           ++    + YT TVGLVCQNY GLFRRPRGMYFSA D+GEMMSM++N
Sbjct: 121 IKDFAPIIYTPTVGLVCQNYSGLFRRPRGMYFSAKDKGEMMSMIFN 166

[Close]

BLAST of MC05g0231 vs. ExPASy Swiss-Prot
Match: P37221 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.4e-13
Identity = 34/40 (85.00%), Postives = 35/40 (87.50%), Query Frame = 0


Query: 7   LYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 47
           + YT TVGLVCQ Y GLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 144 IVYTPTVGLVCQKYSGLFRRPRGMYFSAEDRGEMMSMVYN 183

[Close]

BLAST of MC05g0231 vs. ExPASy Swiss-Prot
Match: P37224 (NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochondriacus OX=28502 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-11
Identity = 30/37 (81.08%), Postives = 33/37 (89.19%), Query Frame = 0


Query: 10  TATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 47
           T TVGLVCQ + GL+RRPRGMYFS+ DRGEMMSMVYN
Sbjct: 144 TPTVGLVCQKFSGLYRRPRGMYFSSDDRGEMMSMVYN 180

[Close]

BLAST of MC05g0231 vs. NCBI nr
Match: PKU80052.1 (NAD-dependent malic enzyme 1, mitochondrial [Dendrobium catenatum])

HSP 1 Score: 80.9 bits (198), Expect = 1.44e-18
Identity = 36/40 (90.00%), Postives = 37/40 (92.50%), Query Frame = 0


Query: 7  LYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 46
          + YT TVGLVCQNY GLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 18 IVYTPTVGLVCQNYSGLFRRPRGMYFSAADRGEMMSMVYN 57

[Close]

BLAST of MC05g0231 vs. NCBI nr
Match: VVV74331.1 (unnamed protein product, partial [Nymphaea colorata])

HSP 1 Score: 80.1 bits (196), Expect = 1.74e-18
Identity = 34/46 (73.91%), Postives = 39/46 (84.78%), Query Frame = 0


Query: 1  LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 46
          ++    + YT TVGLVCQNYGGLFRRPRGMYFSA D+GEMMSM+YN
Sbjct: 6  IQDFAPIIYTPTVGLVCQNYGGLFRRPRGMYFSAKDKGEMMSMIYN 51

[Close]

BLAST of MC05g0231 vs. NCBI nr
Match: PKI51355.1 (hypothetical protein CRG98_028216 [Punica granatum])

HSP 1 Score: 81.6 bits (200), Expect = 8.74e-18
Identity = 36/46 (78.26%), Postives = 39/46 (84.78%), Query Frame = 0


Query: 1   LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 46
           ++    + YT TVGLVCQNYGGLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 56  IKEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAEDRGEMMSMVYN 101

[Close]

BLAST of MC05g0231 vs. NCBI nr
Match: KCW73248.1 (hypothetical protein EUGRSUZ_E017132 [Eucalyptus grandis])

HSP 1 Score: 83.6 bits (205), Expect = 2.38e-17
Identity = 37/46 (80.43%), Postives = 40/46 (86.96%), Query Frame = 0


Query: 1   LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 46
           ++    + YT TVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 142 IKEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 187

[Close]

BLAST of MC05g0231 vs. NCBI nr
Match: KCW73247.1 (hypothetical protein EUGRSUZ_E017132, partial [Eucalyptus grandis])

HSP 1 Score: 83.6 bits (205), Expect = 3.92e-17
Identity = 37/46 (80.43%), Postives = 40/46 (86.96%), Query Frame = 0


Query: 1   LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 46
           ++    + YT TVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 142 IKEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 187

[Close]

BLAST of MC05g0231 vs. ExPASy TrEMBL
Match: A0A2I0WWL9 (NAD-dependent malic enzyme 1, mitochondrial OS=Dendrobium catenatum OX=906689 GN=NAD-ME1 PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 6.95e-19
Identity = 36/40 (90.00%), Postives = 37/40 (92.50%), Query Frame = 0


Query: 7  LYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 46
          + YT TVGLVCQNY GLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 18 IVYTPTVGLVCQNYSGLFRRPRGMYFSAADRGEMMSMVYN 57

[Close]

BLAST of MC05g0231 vs. ExPASy TrEMBL
Match: A0A5K0Y868 (malic domain-containing protein (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS7734 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 8.42e-19
Identity = 34/46 (73.91%), Postives = 39/46 (84.78%), Query Frame = 0


Query: 1  LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 46
          ++    + YT TVGLVCQNYGGLFRRPRGMYFSA D+GEMMSM+YN
Sbjct: 6  IQDFAPIIYTPTVGLVCQNYGGLFRRPRGMYFSAKDKGEMMSMIYN 51

[Close]

BLAST of MC05g0231 vs. ExPASy TrEMBL
Match: A0A2I0J546 (malic domain-containing protein OS=Punica granatum OX=22663 GN=CRG98_028216 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 4.23e-18
Identity = 36/46 (78.26%), Postives = 39/46 (84.78%), Query Frame = 0


Query: 1   LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 46
           ++    + YT TVGLVCQNYGGLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 56  IKEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAEDRGEMMSMVYN 101

[Close]

BLAST of MC05g0231 vs. ExPASy TrEMBL
Match: A0A059C453 (Malic enzyme OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_E017132 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.15e-17
Identity = 37/46 (80.43%), Postives = 40/46 (86.96%), Query Frame = 0


Query: 1   LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 46
           ++    + YT TVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 142 IKEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 187

[Close]

BLAST of MC05g0231 vs. ExPASy TrEMBL
Match: A0A059C4F9 (Malic enzyme (Fragment) OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_E017132 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.90e-17
Identity = 37/46 (80.43%), Postives = 40/46 (86.96%), Query Frame = 0


Query: 1   LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 46
           ++    + YT TVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN
Sbjct: 142 IKEYAPIVYTPTVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 187

[Close]

BLAST of MC05g0231 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 78.2 bits (191), Expect = 1.9e-15
Identity = 35/40 (87.50%), Postives = 36/40 (90.00%), Query Frame = 0


Query: 7   LYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 47
           + YT TVGLVCQNY GLFRRPRGMYFSA DRGEMMSMVYN
Sbjct: 141 IVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYN 180

[Close]

BLAST of MC05g0231 vs. TAIR 10
Match: AT4G00570.1 (NAD-dependent malic enzyme 2 )

HSP 1 Score: 76.6 bits (187), Expect = 5.7e-15
Identity = 32/46 (69.57%), Postives = 38/46 (82.61%), Query Frame = 0


Query: 1   LRSMQQLYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 47
           ++    + YT TVGLVCQNY GL+RRPRGMYFSA D+GEMMSM+YN
Sbjct: 128 IKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYN 173

[Close]

BLAST of MC05g0231 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 48.1 bits (113), Expect = 2.2e-06
Identity = 17/38 (44.74%), Postives = 28/38 (73.68%), Query Frame = 0


Query: 9   YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 47
           YT TVG  CQ YG ++RRP+G+Y S  ++G+++ ++ N
Sbjct: 136 YTPTVGEACQKYGSIYRRPQGLYISLKEKGKILEVLKN 173

[Close]

BLAST of MC05g0231 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 47.8 bits (112), Expect = 2.8e-06
Identity = 17/40 (42.50%), Postives = 29/40 (72.50%), Query Frame = 0


Query: 7   LYYTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 47
           + YT TVG  CQ +G +FRRP+G++ S  D+G+++ ++ N
Sbjct: 127 IVYTPTVGEACQKFGSIFRRPQGLFISLKDKGKILDVLKN 166

[Close]

BLAST of MC05g0231 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 47.8 bits (112), Expect = 2.8e-06
Identity = 17/38 (44.74%), Postives = 28/38 (73.68%), Query Frame = 0


Query: 9   YTATVGLVCQNYGGLFRRPRGMYFSAADRGEMMSMVYN 47
           YT TVG  CQ YG +FR+P+G+Y S  ++G+++ ++ N
Sbjct: 136 YTPTVGEACQKYGSIFRKPQGLYISLNEKGKILEVLKN 173

The following BLAST results are available for this feature:

BLAST of MC05g0231 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIU0	2.7e-14	87.50	NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N...	[more]
Q8L7K9	8.0e-14	69.57	NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N...	[more]
P37225	1.0e-13	69.57	NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX...	[more]
P37221	1.4e-13	85.00	NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX...	[more]
P37224	1.7e-11	81.08	NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochond...	[more]

BLAST of MC05g0231 vs. NCBI nr


Match Name	E-value	Identity	Description
PKU80052.1	1.44e-18	90.00	NAD-dependent malic enzyme 1, mitochondrial [Dendrobium catenatum]	[more]
VVV74331.1	1.74e-18	73.91	unnamed protein product, partial [Nymphaea colorata]	[more]
PKI51355.1	8.74e-18	78.26	hypothetical protein CRG98_028216 [Punica granatum]	[more]
KCW73248.1	2.38e-17	80.43	hypothetical protein EUGRSUZ_E017132 [Eucalyptus grandis]	[more]
KCW73247.1	3.92e-17	80.43	hypothetical protein EUGRSUZ_E017132, partial [Eucalyptus grandis]	[more]

BLAST of MC05g0231 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2I0WWL9	6.95e-19	90.00	NAD-dependent malic enzyme 1, mitochondrial OS=Dendrobium catenatum OX=906689 GN...	[more]
A0A5K0Y868	8.42e-19	73.91	malic domain-containing protein (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM...	[more]
A0A2I0J546	4.23e-18	78.26	malic domain-containing protein OS=Punica granatum OX=22663 GN=CRG98_028216 PE=4...	[more]
A0A059C453	1.15e-17	80.43	Malic enzyme OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_E017132 PE=3 SV=1	[more]
A0A059C4F9	1.90e-17	80.43	Malic enzyme (Fragment) OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_E017132 PE=3 S...	[more]

BLAST of MC05g0231 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G13560.1	1.9e-15	87.50	NAD-dependent malic enzyme 1	[more]
AT4G00570.1	5.7e-15	69.57	NAD-dependent malic enzyme 2	[more]
AT5G25880.1	2.2e-06	44.74	NADP-malic enzyme 3	[more]
AT2G19900.1	2.8e-06	42.50	NADP-malic enzyme 1	[more]
AT5G11670.1	2.8e-06	44.74	NADP-malic enzyme 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037062	Malic enzyme, N-terminal domain superfamily	GENE3D	3.40.50.10380		coord: 1..46 e-value: 6.3E-13 score: 50.4
IPR012301	Malic enzyme, N-terminal domain	PFAM	PF00390	malic	coord: 3..46 e-value: 5.9E-8 score: 32.7
None	No IPR available	PANTHER	PTHR23406:SF78	MALIC ENZYME	coord: 6..46
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 6..46
None	No IPR available	SUPERFAMILY	53223	Aminoacid dehydrogenase-like, N-terminal domain	coord: 3..46

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC05g0231.1	MC05g0231.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006108	malate metabolic process
biological_process	GO:0006090	pyruvate metabolic process
molecular_function	GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051287	NAD binding