MC02g0853 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g0853
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionActin-depolymerizing factor
LocationMC02: 6768123 .. 6769544 (-)
RNA-Seq ExpressionMC02g0853
SyntenyMC02g0853
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGAGGGACAAAAAATGTAATTATGGTGAATTAATGAGGGCATTTTGGTGAGGCGATCTTTATATAAAGGCCCCGACCCGGTTCACTCTCAGTCACGGTAGTCAAAATTCAGAGTTTCAGAGTTCAGAGTATTCCCCGACGAGAGATTTCTCACCATGGCTATGGCTTTCAAGATGGTACCGATTTATGCAATCGAATGCATACTCTCTTTTCTTTTTTTGTTTCTCGATTCTCTGGAATTTTTGGTTCTGATTTTGCTAATGGCGGATATGATCTCAGGCAACCACTGGAATGTGGGTGAGCGATGAGTGCAAGAATTCGTTCATGGAAATGAAATGGAAGAAGGTGCATAGGTATATCGTGTTTAAGATTGACGAAGGATCGAGGTTAGTCACCGTCGATAAGGTCGGCGGTCCGGCCGAAAGCTACGATGATCTCACCGCCTCATTGCCGACCGACGATTGTCGATATGCGGTGTTCGATTTCGACTTCGTCACCGTTGATAATTGCCGGAAGAGCAAGATCTTCTTCATCGCGTGGTCTGTTACTTTTCTCGAATCTTTTTCTGCTTTTCGTCTATCGCATCCCTTCTTCGTTTCATGATCTAACCGCTGCTGATTTAATCCACTCTGATCACTTGAATCTTGCAATACACACTTCATTTCGGGCCGGAATTCTGATAACTTCGATTGAAATCCTCCTCAAATTCATAATCTCTAATTCACTACTGCATATCATGTTACAGAGTCTAGTTAGATGTTCAAAGCAGCACAATAGCACGTTCTTTCGTAATCGGAAGTCGAATTTCTCTAACCGATGATGTATGATTGTTCGTTTTGTACAAGAAATTGGCAATCGCGACTATATATTTATTTTTCTGAAATAGGAAAAACTCCAAGGATTTGGAGGATGGATCTGAATCTGAAACCGATTGGCTTGGTTTGGTTTGGTGCAGGTCGCCCACCGCATCAAGAATTAGAGCGAAAATTCTATACGCAACTTCAAAAGCTGGGCTGAGGGGAGTGCTGGAAGGTATCCATTACGAAGTCCAAGCCACCGATCCGACTGAGATGGGGATGGATGTGATCAAGGACAGAGCCAAATAGATAATAATAATCAGAAAAAATAGCTAATCATCGATCTTTAGGCATCTTTTTTTACTTTGTTTTTAAATAATGAATGACCACTCCAACCATATAGTTATTGTCTGCTTTTTATTTCTTGTTTGTTCACTCTCTCTTTTACTTTGGATTTCTAGATTTTGATTTTGGGCACTAATGTTGTAAGTAAAAGAAAAAATATATATATATATATATCTTCTAATGTTGAGGACAATTGTATTAAAGCCCCTTTTCTCTGTATTGGCTTTAATCTTGAACAGGTGAGCTGATAATTTATGTAATGTTTAATTTAATCTTGCAG

mRNA sequence

TGGAGGGACAAAAAATGTAATTATGGTGAATTAATGAGGGCATTTTGGTGAGGCGATCTTTATATAAAGGCCCCGACCCGGTTCACTCTCAGTCACGGTAGTCAAAATTCAGAGTTTCAGAGTTCAGAGTATTCCCCGACGAGAGATTTCTCACCATGGCTATGGCTTTCAAGATGGCAACCACTGGAATGTGGGTGAGCGATGAGTGCAAGAATTCGTTCATGGAAATGAAATGGAAGAAGGTGCATAGGTATATCGTGTTTAAGATTGACGAAGGATCGAGGTTAGTCACCGTCGATAAGGTCGGCGGTCCGGCCGAAAGCTACGATGATCTCACCGCCTCATTGCCGACCGACGATTGTCGATATGCGGTGTTCGATTTCGACTTCGTCACCGTTGATAATTGCCGGAAGAGCAAGATCTTCTTCATCGCGTGGTCGCCCACCGCATCAAGAATTAGAGCGAAAATTCTATACGCAACTTCAAAAGCTGGGCTGAGGGGAGTGCTGGAAGGTATCCATTACGAAGTCCAAGCCACCGATCCGACTGAGATGGGGATGGATGTGATCAAGGACAGAGCCAAATAGATAATAATAATCAGAAAAAATAGCTAATCATCGATCTTTAGGCATCTTTTTTTACTTTGTTTTTAAATAATGAATGACCACTCCAACCATATAGTTATTGTCTGCTTTTTATTTCTTGTTTGTTCACTCTCTCTTTTACTTTGGATTTCTAGATTTTGATTTTGGGCACTAATGTTGTAAGTAAAAGAAAAAATATATATATATATATATCTTCTAATGTTGAGGACAATTGTATTAAAGCCCCTTTTCTCTGTATTGGCTTTAATCTTGAACAGGTGAGCTGATAATTTATGTAATGTTTAATTTAATCTTGCAG

Coding sequence (CDS)

ATGGCTATGGCTTTCAAGATGGCAACCACTGGAATGTGGGTGAGCGATGAGTGCAAGAATTCGTTCATGGAAATGAAATGGAAGAAGGTGCATAGGTATATCGTGTTTAAGATTGACGAAGGATCGAGGTTAGTCACCGTCGATAAGGTCGGCGGTCCGGCCGAAAGCTACGATGATCTCACCGCCTCATTGCCGACCGACGATTGTCGATATGCGGTGTTCGATTTCGACTTCGTCACCGTTGATAATTGCCGGAAGAGCAAGATCTTCTTCATCGCGTGGTCGCCCACCGCATCAAGAATTAGAGCGAAAATTCTATACGCAACTTCAAAAGCTGGGCTGAGGGGAGTGCTGGAAGGTATCCATTACGAAGTCCAAGCCACCGATCCGACTGAGATGGGGATGGATGTGATCAAGGACAGAGCCAAATAG

Protein sequence

MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDLTASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEGIHYEVQATDPTEMGMDVIKDRAK
Homology
[Close]
BLAST of MC02g0853 vs. ExPASy Swiss-Prot
Match: Q9ZNT3 (Actin-depolymerizing factor 5 OS=Arabidopsis thaliana OX=3702 GN=ADF5 PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.1e-64
Identity = 121/143 (84.62%), Postives = 129/143 (90.21%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGM V+DEC +SFM+MKWKKVHRYIVFKI+E SR VTVDKVGG  ESY DL
Sbjct: 1   MAMAFKMATTGMRVTDECTSSFMDMKWKKVHRYIVFKIEEKSRKVTVDKVGGAGESYHDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
             SLP DDCRYAVFDFDFVTVDNCRKSKIFFIAWSP AS+IRAKILYATSK GLR VLEG
Sbjct: 61  EDSLPVDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPEASKIRAKILYATSKDGLRRVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 144
           IHYE+QATDPTEMG D+I+DRAK
Sbjct: 121 IHYELQATDPTEMGFDIIQDRAK 143

[Close]
BLAST of MC02g0853 vs. ExPASy Swiss-Prot
Match: Q10P87 (Actin-depolymerizing factor 5 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF5 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 5.4e-61
Identity = 112/143 (78.32%), Postives = 127/143 (88.81%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMA+KMAT GM V +EC+  FMEMKWKKVHR++V+KIDE SR V VDKVGGP E Y++L
Sbjct: 1   MAMAYKMATEGMNVKEECQRWFMEMKWKKVHRFVVYKIDERSRAVLVDKVGGPGEGYEEL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
            A+LPTDDCRYAVFDFDFVTVDNC+KSKIFFIAWSPTASRIRAKILYATSK GLR VL+G
Sbjct: 61  VAALPTDDCRYAVFDFDFVTVDNCQKSKIFFIAWSPTASRIRAKILYATSKQGLRRVLDG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 144
           +HYEVQATD +EMG DVI+ RA+
Sbjct: 121 VHYEVQATDSSEMGYDVIRGRAQ 143

[Close]
BLAST of MC02g0853 vs. ExPASy Swiss-Prot
Match: O49606 (Actin-depolymerizing factor 9 OS=Arabidopsis thaliana OX=3702 GN=ADF9 PE=2 SV=2)

HSP 1 Score: 234.2 bits (596), Expect = 9.3e-61
Identity = 108/141 (76.60%), Postives = 124/141 (87.94%), Query Frame = 0 

Query: 3   MAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDLTA 62
           MA K AT+GMW++D+CK SFMEMKWKKVHRY+V+K++E SR VTVDKVG   ESYDDL A
Sbjct: 1   MALKTATSGMWMTDDCKKSFMEMKWKKVHRYVVYKLEEKSRKVTVDKVGAAGESYDDLAA 60

Query: 63  SLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEGIH 122
           SLP DDCRYAVFDFD+VTVDNCR SKIFFI WSP ASRIR K++YATSK+GLR VL+G+H
Sbjct: 61  SLPEDDCRYAVFDFDYVTVDNCRMSKIFFITWSPEASRIREKMMYATSKSGLRRVLDGVH 120

Query: 123 YEVQATDPTEMGMDVIKDRAK 144
           YE+QATDPTEMG D I+DRAK
Sbjct: 121 YELQATDPTEMGFDKIQDRAK 141

[Close]
BLAST of MC02g0853 vs. ExPASy Swiss-Prot
Match: Q8SAG3 (Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 4.8e-49
Identity = 88/135 (65.19%), Postives = 115/135 (85.19%), Query Frame = 0 

Query: 8   ATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDLTASLPTD 67
           A++GM V+D  KN+F+E+K KKVHRY++FKIDE  + V V+K GGPAES+D+  A+LP +
Sbjct: 8   ASSGMGVADHSKNTFLELKRKKVHRYVIFKIDEKKKEVVVEKTGGPAESFDEFAAALPEN 67

Query: 68  DCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEGIHYEVQA 127
           DCRYAV+DFDFVT +NC+KSKIFFIAWSP +SRIRAK+LYATSK   R  L+G+HYE+QA
Sbjct: 68  DCRYAVYDFDFVTSENCQKSKIFFIAWSPDSSRIRAKMLYATSKERFRRELDGVHYEIQA 127

Query: 128 TDPTEMGMDVIKDRA 143
           TDPTEM ++V+++RA
Sbjct: 128 TDPTEMDLEVLRERA 142

[Close]
BLAST of MC02g0853 vs. ExPASy Swiss-Prot
Match: Q9ZSK2 (Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 3.8e-46
Identity = 85/135 (62.96%), Postives = 109/135 (80.74%), Query Frame = 0 

Query: 8   ATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDLTASLPTD 67
           A +GM V+DE K +F+E++ KK HRY+VFKIDE  + V V+K G P ESYDD  ASLP +
Sbjct: 11  AISGMGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLASLPDN 70

Query: 68  DCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEGIHYEVQA 127
           DCRYAV+DFDFVT +NC+KSKIFF AWSP+ S IRAK+LY+TSK  L   L+GIHYE+QA
Sbjct: 71  DCRYAVYDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGIHYEIQA 130

Query: 128 TDPTEMGMDVIKDRA 143
           TDPTE+ ++V+++RA
Sbjct: 131 TDPTEVDLEVLRERA 145

[Close]
BLAST of MC02g0853 vs. NCBI nr
Match: XP_022146103.1 (actin-depolymerizing factor 5-like [Momordica charantia])

HSP 1 Score: 290 bits (743), Expect = 4.95e-99
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL
Sbjct: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
           TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG
Sbjct: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 143
           IHYEVQATDPTEMGMDVIKDRAK
Sbjct: 121 IHYEVQATDPTEMGMDVIKDRAK 143

[Close]
BLAST of MC02g0853 vs. NCBI nr
Match: XP_022943996.1 (actin-depolymerizing factor 5-like [Cucurbita moschata] >XP_022986745.1 actin-depolymerizing factor 5-like [Cucurbita maxima] >KAG7010856.1 Actin-depolymerizing factor 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 285 bits (730), Expect = 4.76e-97
Identity = 141/143 (98.60%), Postives = 141/143 (98.60%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL
Sbjct: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
           TASLP DDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLR VLEG
Sbjct: 61  TASLPNDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRRVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 143
           IHYEVQATDPTEMGMDVIKDRAK
Sbjct: 121 IHYEVQATDPTEMGMDVIKDRAK 143

[Close]
BLAST of MC02g0853 vs. NCBI nr
Match: XP_022947625.1 (actin-depolymerizing factor 5-like [Cucurbita moschata] >XP_022971127.1 actin-depolymerizing factor 5-like [Cucurbita maxima] >XP_023532678.1 actin-depolymerizing factor 5-like [Cucurbita pepo subsp. pepo] >KAG6604928.1 Actin-depolymerizing factor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 282 bits (722), Expect = 7.91e-96
Identity = 139/143 (97.20%), Postives = 141/143 (98.60%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGG AESYDDL
Sbjct: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGSAESYDDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
           TASLPTDDCRYAVFDFDFVT+DNCRKSKIFFIAWSPTASRIRAKILYATSKAGLR VLEG
Sbjct: 61  TASLPTDDCRYAVFDFDFVTIDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRRVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 143
           IHYEVQATDPTEMG+DVIKDRAK
Sbjct: 121 IHYEVQATDPTEMGIDVIKDRAK 143

[Close]
BLAST of MC02g0853 vs. NCBI nr
Match: XP_038901914.1 (actin-depolymerizing factor 5-like [Benincasa hispida])

HSP 1 Score: 281 bits (719), Expect = 2.27e-95
Identity = 139/143 (97.20%), Postives = 139/143 (97.20%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL
Sbjct: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
           TASLP DDCRYAVFDFDFVTVDNCRKSKIFFIAWSPT SRIRAKILYATSK GLR VLEG
Sbjct: 61  TASLPNDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTESRIRAKILYATSKDGLRRVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 143
           IHYEVQATDPTEMGMDVIKDRAK
Sbjct: 121 IHYEVQATDPTEMGMDVIKDRAK 143

[Close]
BLAST of MC02g0853 vs. NCBI nr
Match: XP_023512924.1 (actin-depolymerizing factor 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 281 bits (719), Expect = 2.27e-95
Identity = 139/143 (97.20%), Postives = 140/143 (97.90%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGG AESYDDL
Sbjct: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGSAESYDDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
           TASLP DDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLR VLEG
Sbjct: 61  TASLPNDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRRVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 143
           IHYE+QATDPTEMGMDVIKDRAK
Sbjct: 121 IHYELQATDPTEMGMDVIKDRAK 143

[Close]
BLAST of MC02g0853 vs. ExPASy TrEMBL
Match: A0A6J1CXN9 (actin-depolymerizing factor 5-like OS=Momordica charantia OX=3673 GN=LOC111015397 PE=3 SV=1)

HSP 1 Score: 290 bits (743), Expect = 2.40e-99
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL
Sbjct: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
           TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG
Sbjct: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 143
           IHYEVQATDPTEMGMDVIKDRAK
Sbjct: 121 IHYEVQATDPTEMGMDVIKDRAK 143

[Close]
BLAST of MC02g0853 vs. ExPASy TrEMBL
Match: A0A6J1JEW6 (actin-depolymerizing factor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111484412 PE=3 SV=1)

HSP 1 Score: 285 bits (730), Expect = 2.31e-97
Identity = 141/143 (98.60%), Postives = 141/143 (98.60%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL
Sbjct: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
           TASLP DDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLR VLEG
Sbjct: 61  TASLPNDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRRVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 143
           IHYEVQATDPTEMGMDVIKDRAK
Sbjct: 121 IHYEVQATDPTEMGMDVIKDRAK 143

[Close]
BLAST of MC02g0853 vs. ExPASy TrEMBL
Match: A0A6J1FT84 (actin-depolymerizing factor 5-like OS=Cucurbita moschata OX=3662 GN=LOC111448560 PE=3 SV=1)

HSP 1 Score: 285 bits (730), Expect = 2.31e-97
Identity = 141/143 (98.60%), Postives = 141/143 (98.60%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL
Sbjct: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
           TASLP DDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLR VLEG
Sbjct: 61  TASLPNDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRRVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 143
           IHYEVQATDPTEMGMDVIKDRAK
Sbjct: 121 IHYEVQATDPTEMGMDVIKDRAK 143

[Close]
BLAST of MC02g0853 vs. ExPASy TrEMBL
Match: A0A6J1I143 (actin-depolymerizing factor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111469893 PE=3 SV=1)

HSP 1 Score: 282 bits (722), Expect = 3.83e-96
Identity = 139/143 (97.20%), Postives = 141/143 (98.60%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGG AESYDDL
Sbjct: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGSAESYDDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
           TASLPTDDCRYAVFDFDFVT+DNCRKSKIFFIAWSPTASRIRAKILYATSKAGLR VLEG
Sbjct: 61  TASLPTDDCRYAVFDFDFVTIDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRRVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 143
           IHYEVQATDPTEMG+DVIKDRAK
Sbjct: 121 IHYEVQATDPTEMGIDVIKDRAK 143

[Close]
BLAST of MC02g0853 vs. ExPASy TrEMBL
Match: A0A6J1G7F5 (actin-depolymerizing factor 5-like OS=Cucurbita moschata OX=3662 GN=LOC111451431 PE=3 SV=1)

HSP 1 Score: 282 bits (722), Expect = 3.83e-96
Identity = 139/143 (97.20%), Postives = 141/143 (98.60%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGG AESYDDL
Sbjct: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGSAESYDDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
           TASLPTDDCRYAVFDFDFVT+DNCRKSKIFFIAWSPTASRIRAKILYATSKAGLR VLEG
Sbjct: 61  TASLPTDDCRYAVFDFDFVTIDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRRVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 143
           IHYEVQATDPTEMG+DVIKDRAK
Sbjct: 121 IHYEVQATDPTEMGIDVIKDRAK 143

[Close]
BLAST of MC02g0853 vs. TAIR 10
Match: AT2G16700.1 (actin depolymerizing factor 5 )

HSP 1 Score: 247.3 bits (630), Expect = 7.5e-66
Identity = 121/143 (84.62%), Postives = 129/143 (90.21%), Query Frame = 0 

Query: 1   MAMAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDL 60
           MAMAFKMATTGM V+DEC +SFM+MKWKKVHRYIVFKI+E SR VTVDKVGG  ESY DL
Sbjct: 1   MAMAFKMATTGMRVTDECTSSFMDMKWKKVHRYIVFKIEEKSRKVTVDKVGGAGESYHDL 60

Query: 61  TASLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEG 120
             SLP DDCRYAVFDFDFVTVDNCRKSKIFFIAWSP AS+IRAKILYATSK GLR VLEG
Sbjct: 61  EDSLPVDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPEASKIRAKILYATSKDGLRRVLEG 120

Query: 121 IHYEVQATDPTEMGMDVIKDRAK 144
           IHYE+QATDPTEMG D+I+DRAK
Sbjct: 121 IHYELQATDPTEMGFDIIQDRAK 143

[Close]
BLAST of MC02g0853 vs. TAIR 10
Match: AT4G34970.1 (actin depolymerizing factor 9 )

HSP 1 Score: 234.2 bits (596), Expect = 6.6e-62
Identity = 108/141 (76.60%), Postives = 124/141 (87.94%), Query Frame = 0 

Query: 3   MAFKMATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDLTA 62
           MA K AT+GMW++D+CK SFMEMKWKKVHRY+V+K++E SR VTVDKVG   ESYDDL A
Sbjct: 1   MALKTATSGMWMTDDCKKSFMEMKWKKVHRYVVYKLEEKSRKVTVDKVGAAGESYDDLAA 60

Query: 63  SLPTDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEGIH 122
           SLP DDCRYAVFDFD+VTVDNCR SKIFFI WSP ASRIR K++YATSK+GLR VL+G+H
Sbjct: 61  SLPEDDCRYAVFDFDYVTVDNCRMSKIFFITWSPEASRIREKMMYATSKSGLRRVLDGVH 120

Query: 123 YEVQATDPTEMGMDVIKDRAK 144
           YE+QATDPTEMG D I+DRAK
Sbjct: 121 YELQATDPTEMGFDKIQDRAK 141

[Close]
BLAST of MC02g0853 vs. TAIR 10
Match: AT2G16700.2 (actin depolymerizing factor 5 )

HSP 1 Score: 226.9 bits (577), Expect = 1.1e-59
Identity = 110/132 (83.33%), Postives = 118/132 (89.39%), Query Frame = 0 

Query: 12  MWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDLTASLPTDDCRY 71
           M V+DEC +SFM+MKWKKVHRYIVFKI+E SR VTVDKVGG  ESY DL  SLP DDCRY
Sbjct: 1   MRVTDECTSSFMDMKWKKVHRYIVFKIEEKSRKVTVDKVGGAGESYHDLEDSLPVDDCRY 60

Query: 72  AVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEGIHYEVQATDPT 131
           AVFDFDFVTVDNCRKSKIFFIAWSP AS+IRAKILYATSK GLR VLEGIHYE+QATDPT
Sbjct: 61  AVFDFDFVTVDNCRKSKIFFIAWSPEASKIRAKILYATSKDGLRRVLEGIHYELQATDPT 120

Query: 132 EMGMDVIKDRAK 144
           EMG D+I+DRAK
Sbjct: 121 EMGFDIIQDRAK 132

[Close]
BLAST of MC02g0853 vs. TAIR 10
Match: AT2G31200.1 (actin depolymerizing factor 6 )

HSP 1 Score: 185.7 bits (470), Expect = 2.7e-47
Identity = 85/135 (62.96%), Postives = 109/135 (80.74%), Query Frame = 0 

Query: 8   ATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDLTASLPTD 67
           A +GM V+DE K +F+E++ KK HRY+VFKIDE  + V V+K G P ESYDD  ASLP +
Sbjct: 11  AISGMGVADESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLASLPDN 70

Query: 68  DCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEGIHYEVQA 127
           DCRYAV+DFDFVT +NC+KSKIFF AWSP+ S IRAK+LY+TSK  L   L+GIHYE+QA
Sbjct: 71  DCRYAVYDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGIHYEIQA 130

Query: 128 TDPTEMGMDVIKDRA 143
           TDPTE+ ++V+++RA
Sbjct: 131 TDPTEVDLEVLRERA 145

[Close]
BLAST of MC02g0853 vs. TAIR 10
Match: AT4G00680.1 (actin depolymerizing factor 8 )

HSP 1 Score: 177.2 bits (448), Expect = 9.6e-45
Identity = 79/134 (58.96%), Postives = 108/134 (80.60%), Query Frame = 0 

Query: 8   ATTGMWVSDECKNSFMEMKWKKVHRYIVFKIDEGSRLVTVDKVGGPAESYDDLTASLPTD 67
           + +GM V+DECK  F+E+K K+ +R+IVFKIDE ++ V ++K+G P E+YDD T+S+P D
Sbjct: 4   SASGMHVNDECKIKFLELKAKRTYRFIVFKIDEKAQQVQIEKLGNPEETYDDFTSSIPDD 63

Query: 68  DCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIRAKILYATSKAGLRGVLEGIHYEVQA 127
           +CRYAV+DFDF T DNC+KSKIFFIAWSP  SR+R+K+LYA+SK   +  +EGI  E+QA
Sbjct: 64  ECRYAVYDFDFTTEDNCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKREMEGIQVELQA 123

Query: 128 TDPTEMGMDVIKDR 142
           TDP+EM +D+IK R
Sbjct: 124 TDPSEMSLDIIKGR 137

The following BLAST results are available for this feature:
BLAST of MC02g0853 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9ZNT31.1e-6484.62Actin-depolymerizing factor 5 OS=Arabidopsis thaliana OX=3702 GN=ADF5 PE=1 SV=1[more]
Q10P875.4e-6178.32Actin-depolymerizing factor 5 OS=Oryza sativa subsp. japonica OX=39947 GN=ADF5 P... [more]
O496069.3e-6176.60Actin-depolymerizing factor 9 OS=Arabidopsis thaliana OX=3702 GN=ADF9 PE=2 SV=2[more]
Q8SAG34.8e-4965.19Actin-depolymerizing factor OS=Vitis vinifera OX=29760 PE=2 SV=1[more]
Q9ZSK23.8e-4662.96Actin-depolymerizing factor 6 OS=Arabidopsis thaliana OX=3702 GN=ADF6 PE=1 SV=1[more]
BLAST of MC02g0853 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022146103.14.95e-99100.00actin-depolymerizing factor 5-like [Momordica charantia][more]
XP_022943996.14.76e-9798.60actin-depolymerizing factor 5-like [Cucurbita moschata] >XP_022986745.1 actin-de... [more]
XP_022947625.17.91e-9697.20actin-depolymerizing factor 5-like [Cucurbita moschata] >XP_022971127.1 actin-de... [more]
XP_038901914.12.27e-9597.20actin-depolymerizing factor 5-like [Benincasa hispida][more]
XP_023512924.12.27e-9597.20actin-depolymerizing factor 5-like [Cucurbita pepo subsp. pepo][more]
BLAST of MC02g0853 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CXN92.40e-99100.00actin-depolymerizing factor 5-like OS=Momordica charantia OX=3673 GN=LOC11101539... [more]
A0A6J1JEW62.31e-9798.60actin-depolymerizing factor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111484412 P... [more]
A0A6J1FT842.31e-9798.60actin-depolymerizing factor 5-like OS=Cucurbita moschata OX=3662 GN=LOC111448560... [more]
A0A6J1I1433.83e-9697.20actin-depolymerizing factor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111469893 P... [more]
A0A6J1G7F53.83e-9697.20actin-depolymerizing factor 5-like OS=Cucurbita moschata OX=3662 GN=LOC111451431... [more]
BLAST of MC02g0853 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G16700.17.5e-6684.62actin depolymerizing factor 5 [more]
AT4G34970.16.6e-6276.60actin depolymerizing factor 9 [more]
AT2G16700.21.1e-5983.33actin depolymerizing factor 5 [more]
AT2G31200.12.7e-4762.96actin depolymerizing factor 6 [more]
AT4G00680.19.6e-4558.96actin depolymerizing factor 8 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002108Actin-depolymerising factor homology domainSMARTSM00102adf_2coord: 16..143
e-value: 7.7E-48
score: 174.9
IPR002108Actin-depolymerising factor homology domainPFAMPF00241Cofilin_ADFcoord: 18..140
e-value: 2.3E-31
score: 108.1
IPR002108Actin-depolymerising factor homology domainPROSITEPS51263ADF_Hcoord: 11..143
score: 35.106728
IPR029006ADF-H/Gelsolin-like domain superfamilyGENE3D3.40.20.10Severincoord: 8..143
e-value: 1.1E-43
score: 150.3
NoneNo IPR availablePANTHERPTHR11913:SF98ACTIN DEPOLYMERIZING FACTOR 5-RELATEDcoord: 5..143
NoneNo IPR availableSUPERFAMILY55753Actin depolymerizing proteinscoord: 6..141
IPR017904ADF/CofilinPANTHERPTHR11913COFILIN-RELATEDcoord: 5..143
IPR017904ADF/CofilinCDDcd11286ADF_cofilin_likecoord: 10..142
e-value: 7.89647E-58
score: 174.282

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g0853.1MC02g0853.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051017 actin filament bundle assembly
biological_process GO:0030042 actin filament depolymerization
cellular_component GO:0015629 actin cytoskeleton
cellular_component GO:0005737 cytoplasm
molecular_function GO:0051015 actin filament binding
molecular_function GO:0003779 actin binding