MC04g0408 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC04g0408
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A
LocationMC04: 3249614 .. 3258884 (-)
RNA-Seq ExpressionMC04g0408
SyntenyMC04g0408
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAATTTCTTCCATTCTTGGGAAAAGGAAAAAAAAGGAAAGAAAAAGAAAAATCTATTAAGTCATATTATATTATTATATTCGATAAGGAAATGTGAGAAATTCATCATCAGTCGTTGATTCTTAACGTGAGAGATTCGTGGACCGTCCGATGAGAATCTTGCATTCCCCAGTTTATAACGTTGGAGAAGCAACTTCGGCCACAAATTTGCTGCAAACCAACGCAAAACAGAACCCCAATTCTCACAGAGCAACAATGGCGGCAATGGTATCTCCATGGGGAAAGCCAGGCGCGTGGGCCCTCGACTCGGAGGAACACGAAGCTCAACTTCTGAAAGAAGAGCAGGAGCAGCAGAAACAAGCGGAGCTCGGCCTGGATTTCCCCTCCCTCAACGACGCCTTGGCCACCAAGCAGAAGAAGAAGGGCCAAACTCTCTCGTTGGCGGAGTTCAACGCCTATGGCGGCAGCGGCACCAGATCTTCCGCTCACACCGCTGCGCCCCAGGGCCTCACGCATGAAGATCTCATGGTGCTTCCCACCGGCCCTCGCCAACGGACGGCGGAGGAGCTCGACCGGAACCGCCTCGGCGGTGGCTTTAGGAATTACGGTACGAATGGTTCGTATGAGAGGAATAATAGGTATTCGATTGGGGATGATTCGTCTAATTCGAAGTGGGGTTCGTCTAGGGTTTCTGATGAGAATCGGAGGAACAATGGCGGGGGTAATAGGGGGGATTCGGGTAGGGAAATTGCACCTTCTCGGGCTGATGAGGTCGATAATTGGGCCTTAACTAAGAAACCGATGGCTGCAAATGGATTTGAGAGGAGGGATAGAGGAGGAGGGTTTTCGGATTTGCCGTCTTCGAAAGCCGACGAATCAGAGAGTTGGATTTCCAATAAGAGCAATGTTCCATCGGATGTGCGGAGATTTGGGACTAACGGCGGATTTGAAAGAGAGCGACGAGGAGGTTTTCCGACGGGCGGCGGAGGCGGTGCAGATTTAGAAAATTGGGGGAGGAAGAAAGAGGAAGGTAATAATGGTGGCAGCGTTGCGGGTAGGCCGAAGCTTAATTTGCAGCCACGGACACTGCCAGTGAGCGATGGGAAGTCGTCAGAGATATCAGGGACGGGGCTGGGGGCGAGGTCGAAGAGTTCAAATCCATTCGGAGAGGCGAGGCCGAGGGAACAGGTTCTGGCTGAGAAAGGACATGACTGGAAAAAACTCGACGAGCAACTGGAGTCGAAGATCAAAGACGAGCAACCAGAGAAGGTAGAAAACTCTAACAGCACTTCCTTTGGAAAGAAGAGCTTTGGCCGGGCATCTGAGGACCGAACAGAGAGGAGCTGGAGGAAGCCAGAGTCGACATCGGAGTCACTGGAATCTCGTCCCGGCAGGTTTGTTCCAGCATCCAATTTCTAGCTTTATAACATAAGTTTCTTCCCTTTTGTTGCCATAATCACATCAGCTAACTTCACAAAAGTATCTTGACATTGTTCTATATTCTGATATGATCTCACCTGATTGTTGCTTTACAGTGCGGAGGAGAATGAAAATGGAGATGTTGAAGGGGAGTGTGAAGGGGACGAAAATGGAGCTTGATGGGATGAGATGATACAGAGAGATTGAAGTTAGTGAAAGTTTTGGAGTGATGAGTGATGACAACATTCTCTAATGTTGTTCTGATCCTTTATAATTTTGCCTCTGAGAAACAATTTTGATATGGTGGTGTTGGGGAGTTCTTGAGTCAGTTACTGTAATGTTTGAAGCCAACTCTTGGAATTAGAAACTACTACTGCTATTGTGCTAGTTAAGTTTAAGATTATTGTTCTAATCTTATCCCACTTATTGTTTAGAGTTGGCTTGCTTTATAACTGGATTTTATGTTGGATTTTAGAATGGGAAAAGAACTTGCTCTGTCTTCTCCATTCTGAATCTCAATGGAGTTGGTGGTTTGTTTTCTTGCAGCTCATCGGTTGAAAACAAAGGGCACCATCCATGGAGCAGGACGCAGAGTTGCCACTTTTAGCTCTGTTCCAAGGCATGCTAGGATGATGGGTAAGTTATCAGCTTAGTTTTCAAGGTTAAAATATTAGTTTCGTAATGGATTTTCAGCTTTGATTCATTTTGGATTCTATATTTTCAACTTTGGTTCATTTTGTTTGAATGTTCGTTTAATGTGAATGTGAAAAGTGACTACTTTGATTTCTATTTTCAAAATTTTAACCCAATTTCTCAACCTAATAAAAGCTCTTTAAAATGAAATCATTGTTATATTTTGAAGAAACGTATTGAATATAGTGTCAAACTGGATATAGATAGATGCTCGGTTGATGTAATGGGCCTCGCTGAAAAGGGCCAAATAGGTATAGTGGGCCTCTTCTTGCCGATCAAAGCCCAAAGGGATGCTCTGTTGAGTAATTTTCTATTTTAATGAGTTAAAAATAAAAGAAAAGGTCCTACCGGGAGTTGAACCCAGGTCGCTGGATTCAAAGTCCAGAGTGCTAACCACTACACCATAGAACCACTTCATTTGGTGATGCAACTTGTACAAAAAAACAAAAAACAAATAGGGTTATGGCTAATTGAGAGTAGAAGATACCCGAGTTCGATCAGTTAAATCCAACGTTGGAGAGGAGAGATTGAGAGGTAGAGAGTGAGAGCGAGAGTTTTAACTGGTCAAAGCATTTGTCTAATATAATCATAAAAAGTTTAGAGATTATGAATATTTTAAAAGGAAGAGTTTGTGTAATTGGGTGAAATTTTGATTCTTTCCTTTCTCTCTTCGTTTTGAGCCATCTTATTTTTGCTGAAGAAAATAAAAATTCCTTTTCCCCTCATTTTTGTGACTCATGTTTCGATAAGTCGGTGTCGATTTTAGTTGATTTCAATCAATCGGAATGAAATGAAAAGATTATGAGAGAGAAGTAGAATGGAGAATAAAAGAAAGCTGAGGAGAAAAGAAAAGAAGAGTGAAATGAGAGAAAGAGGTGAAGGAAAAGAGGGAGAGAGTAGGAGAGATTGAACTTGTTTATTTATCTCATCATTAAATATCTATATGGATAAATTATATATACACACTGAAATATCATCACAATTGTAGGGGTGAGAATTATTTGAATCTAACAAGTTCTAGAGATGTAGTGCTAATTATGATCGATTAGATTAATCATTTTTAACTTATTTAGAATTAATAGTAAAATGATTATTAGTTAATTTAAATATTTTTTCAAATTTTTTATTTTTATATTTTCTCCATAAACATTCACTAACATATATTTCCATAAAATTGAGATCTCGACATTTTCATTGACATCGACATTTGAAACCTTGTCTATATCATACAAATTTGGGAAACAAAAATATTACACAATAAAAAAGGGTTTAGACATCATTTTAAAATTATATCTTATTATATTAAAAAACATATTTATATAAATAGCATAAAATAAATATATATTTTCAATTGCAACCAAGCTTGAAACTTGATCCATCAAGCAGTCTCACTTTCTAGGTTCTTTTTTTTAGGGTACCCGAGAACATATCTAGTGGAAATGGGGTGAAAAAGCTCTAGCATAGCTCTTTCTTTCTCGGGTCTCCTTTCAAAGGAAAAAGGCTGGTGCAGTTAGTAGCTAAAGGTTTCACTCAAACATGTGGTCTCGACGCGAAAAGAGCTTGCTTTCTACTTTCTACGTCTGCATTTTGTGTCCAGCCCGCAAAAGGGAAAATCGTGGAGAGATGTATCATGTCAGGATTCGTTGCTCAAATTGAATCCTTTCTTTATCATTGTTTCTATTCGTATTATTTGTAACTTGCCACTTGATTTGATTCGACTGAACATCTCGACCATTAACGAAAATTTCACCCACATCCGATAGGTTGGGCGCTCGACCAGCTTACAAGTTGGAGGAGGCTCCAACTATCCTTAAATTTTTTTTAAAATAACATAATAAACTCAAAGTATGATGTGTGTATATATAAAAAAAATGTTAAATTCTATATATTTTTAGTTAAATTAAATTCTCAACGGATTGTCCAAGAGACTAGACAAAGTCAACTGTCCAAAGCCTTTCCTTTACAAATCCCAACGGTCTCCCAGTCTCTGTACGCCGCCGCCGTCTCCGGCCACCGCGCCACTCTACGAAAATGCTCGTCTTCTTCCTCGCAGCCTTCTTCCTCTTCACGGCATCCAACTCCTCCCTCTTCCCCATCGACGAAGCCACCATCGCCGATATCCACAGCGCCTTCTCCCAAAACCACCTCACCTCCAGGCAACTCCTCGATCATTACCTCAACAAGATCGACCTCCTCAATCCAGTGCTTCGCAGCGTTCTGGAAGTGAATCCGGACGCCAGAGCTCAAGCGGAGGCCGCCGATCGAGAGCGGGACCTGGCCGGAGGAAAAGCCCTAGGCGAACTTCATGGAATCCCGGTGCTGCTCAAGGATTCCATCGCCACCAAGGGTCCGCTCAACACCACCGCCGGATCCTTCGCATTGCTCGGATCCGTGGTGCCCCGAGATGCGACGGTGGTCGCCCGCCTGAGGAAAGCCGGTGCGGTGATTTTGGGCAAAGCTTCGCTCACCGAGTGGTACGGATCTCGTTCTTTGAAGATTCCCAATGGCTGGTGCGCTCGTGGGGGTCAAGCCCTGGTCAGTAAGTTTTCCAACGGCTGCCCTAATTTTCCGCAATTTCTGAGCCTTCTTTTCAGTAATGGATTAGAATCAATTCAATTCACAGAACCCATATGGGAAAGAAGGGGATCCATGTGGGTCAAGTAGTGGCTCTGCAATATCGGTGGCAGCCAACATGGCGGCAGTGTCGCTGGGAACTGAAACAGATGGCTCAATTCTATGTCCCGCAGATTACAACTCAGTCGTCGGCATCAAACCCACCGTCGGTCTCACGAGCCGGGCCGGCGTTATCCCGGTCACTCCTCGGCAAGACACCATCGGGTATGAAAACTTTAACTGAACCCTTCAAATCTTGGTAATTTTTGTTTCATTTTTCTTTAGAAGTTTGATGGGAGACTGCTGCTGGACAGGCCTATATGCAGGACAGTTTCAGATGCAGTTTATTTGCTTGATGCCATAGTGGGGTTTGACCCAATGGACTCTGAAGCAACTAAAGCAGCATCTGAGTTCATTCCTTGTGGTGGCTACAAGCAATTTCTGAGGAAAGATGGGCTCAAAGGGAAGAGACTGGGCATTGTTAGGCATCCCTTTTTTGACTTGTATACCAACGGCTCCATGGCCATTCCAACCTTTGAACATCATGTCAATCTGCTGAGGTATAACCACAAAATCACCTCTTTTTCTCCATTGACCCACAGATGCTTCGATTTCTTGGTCAAAATTTTAGAGGGAGTTCCTGTCCAGTGAATGTCCTGAACAAAAAGAAGTACTTTTTTAGTTTTCAAAGTTCATTCCTTATTGCACATTAACGAAAATACCAGATTGAATTATATAAACAGAGAATTCTAGAGGTGTAGCCCCTAAGATTTGGCTGACCATGTATTTAGTGAAGCATTGTTAGTGTACTAGACTAGTTGCTGACTTTTTTCCATTTTCCTGCAATTGAAGGAAAAGTGGAGCAACCATTGTGGATAACCTTCAAATATCAAATATAGATACAATTCTGGATCCTTATGGGAGTGGTGAACTTATTGCAACAATAGCTGAGTTCAAGCTCGCCATTAACGATTACCTCAAGAACCTGATCCACAGTCCAGTCAGATCTCTGGCTGACATTATTGCCTTCAACAACAACCACCCTGAACTGGTAAGTTACCTCATTCTGAAGTTGTTTTTCAAAATTCTATAGTAGTAAAACAAATGTTTGGGGATGAGATGAACTATTGATACTTTTTGGGGCATTACAGGAGAAGATGAACGAGTATGGGCAAGACGCCTTTCTTTTATCCGATCAAACAGATGGGATCGGCAAGGCGGAGGAAGAGGCAATTAGTATGATGGCAAACTTATCGAGAGATGGGTTCGAGTTGATGATGAAGACCTATAATCTGGATGCAATGGTAACTCTAGGGACATCAGCTGAATCCGTCCTAGCAATTGGGGGATACCCTGCAATTTCTGTTCCTGCTGGATATGAGGGAAACGGTGATCCATTTGGCATCCTCTTTGGGGGGCTCAGGGGAACCGAAACGAAGCTAATCGAGATCGCTTATGCATTCGAACAAGCGACCATGGTACGAAGGCCTCCTCCTCTGCCTTTTGAATTATTAGATTGCAACTAGAAAAATTTCTAACTGTCTAGTAGGATGTTGTAACCTTTGGAAAAATAAGTTCTCTGCTGGGAGCAATGTTCCTTTTGACTGTATATTTGCTTTCCACTTTTAAGTAGTTGACTAGCTTCCTGTTTCAAATATTATCTCAATGATATTGAATGTGTCCATCACAACGTGGCTACTCCCTAATATCCGGTGGATGCTAATTTGAATATATTTTATATATTTTTTGATATCATGAATAATTTGAATATATTTTATATATTTTTTGGTATCATGAGTATTCATACCAACCTACGTACACCTCAACTAAACCTCACAAGACATATTACAGGACCTCACAACATAGGGTTGTCGATAAACACTAAGATAGCCACTACGAGTTTGAACCCTTGCCCCAATGCCCACCCGTCCATTTCCCGTGCCCCTACTAGGCTAGCTTATGGTAGTTAACTAATTACAATTTTGTTAACAAAAATGGTTGCAAAAAATTTACTCAATTGCTCAAAGTAATTGCCAATGTTTCAGATGTCAGATTACATATTTCTTCCAACTCATCAGTCAACTAAAAATGCCCTTAGATCCCATGGACAAACACAGCATAATATAGCTAATGCTACAAACAGACAAATCCACCGGACATGGACGGCAACGCCCTCCTCCCTCTTTGGACCTCATCAAATCTTCATATTTTTAATGTCCTGTTCTCCACACACTTCTCTTTGTAATCTTTCTATGGCCATGAACCAGAACGGGTACAAGAAAACAGTACTTAAACCAGAAGAGATGAGTATAAAACTTCCATATGAAGCTCCTATATATCATGAAGACTTCCATTTAAGTTTTTCACCAACATGGCCTACACATCCCCGCTTAGTTCTGTAGTCTCGTCATTGCTTCTGATTCTTGTGGCCTTTTGTTTGACTGCCCCACAATCCACGATCGTTCAAGGGCTGTCGATCAGAGAAGCCACCGTGCATGACCTCCAGCTAGCTTTCAAGCAAAACCAGCTCACTTCGAGAGATCTTGTTGAGTTCTACCTAGACGAAACCCGTAGACTCAACCCGGTTGTCCATGGGATCATAGAAATCAACCCAGATGCATTGCTCCAAGCTGACAAGGCTGACCAAGAGCGCAAGGCTAAGAAGCCTGGATCGCTTTGTGGTCTGCATGGAATTCCAGTTCTACTGAAAGATAACATTGGGACTAAGGATAAGTTGAATACTACAGCTGGATCGTTCGCATTGCTTGGCTCTGTTGTGCCACATGATGCGTTCGTGGTAAAGAGACTGAGGAGGGCTGGAGCTATAATTCTGGGAAAGGCTAGCTTGAGTGAATGGGCCGATTTCCGATCTCTTACTGCTCCAGCGGGTTTGAGCGCCAGAGGTGGCCAGGGAAAGGTCAGTTACCTGCTCCAGAGACATTTTCCCCCACATCCAATGCTTTTCAAGTAGAATTAAAGACCATTTCTTTTGTACAATTCTCTTTTCAGAACCCATATATTTTATCAGCATCACCCTGTGGATCCAGCAGCGGGCCATCCATATCAGTTGCAACAAACTTAGCAGCAGTGTCAGTAGGAACCGAGACCGACGGTTCTATCCTCTGTCCAGCTAGTTCTAACTCGGTTGTTGGAATCAAACCAACAGTCGGCCTCACCAGTAGAGCAGGTGTCATTCCAGTTTCCCCTAGACAGGACACGATCGGGTAAGCAATCTTGCAACCATATCAGAAAACTATTGTATAGAGATCAAAAGAACTGAGTCTTTGTAACTTCATATGTGAAAACAGGCCAATCTGCAGGACAGTGACGGATGCCGTAATAGTTCTTGACACCATTGTAGGGTTCGATTACAATGATGTAGCCTCCAGAACAGCCTCAAAGTACATTCCATATGGTGGCTATAAACAATTCCTCAATGCAAATGGGTTAAAGGGGAAGAGATTAGGCATTGTGAGGAGCCCTTTTTTCAGCTTCATCAATGATTCAACCATCACACAGGCTTTTGAGGATCATTTCAACACTCTAAGGTAACTGCCCTAAATCCTTAGCCAAATGGGTTTCTATTCATTTAAACAAAGAGTTGGAATCTATTGACAACTAGAAACCAATTCAATATGAACAACTATCTTCAAGTGGAATAGTTTACGTACCTCTAAAATTATCTTCTTCGGCTGTTCTTCTAGTAAACACTAAATCTAACACGTTGAAATTGAAGGCGAGGTGGTGCCATCTTGATAGACAATCTGGAGATAGCGAATATAGATACAATCTTGAACGCGACTGCAAGTGGGGAAGCAGTGGCGTTGCTCGCTGAATTCAAACAATCTTTGAATGCGTACCTGAAAGAGCTGGTGGCTTCCCCTGTTCGAAGTTTGGGAGACATAATCGCCTTCGATAACGCAGACCCAGATCTGGTAACATAACACTCAATCCATTTTTCTTCGCACAGTAACCGATTTCGTTCGTAATCGATTCGACCCATTGCTGTGCAGGAACTGCTCAAGGTTTTCGGCCAGGAGATCTTTCTGGCGGCGGAAGCGACAAATGGGATTGGCGACGTCCAGAAGGCGGCGCTGTTGAATTTGGCGAGGTTGACGAGAGATGGGTTTGAGAAATTGGTGAAGGAGAAGCGGTTGGACGCGGTGGTGACGCCGGGTTCCGGCATAGCTCCGGTGCTCGCGATTGGGGGTTTCCCCGGAATCAGTGTTCCGGCGGGATATGACGGCGGAGGAGTTCCGTTCGGGATTTGCTTCGGAGGGTTGAAGGGTTCGGAGGGGAAGCTGATTGAGGTCGCATATGGGTTTGAGCGAGCCACTCTGATTCGAAAGCCTCCTTCCTTCAAGCCTTGAACTTTGAAGCAAAGGCTATGAATCAGAATTACTCTTTGAATTCAAATTAATGGTTTTTTTTTTTCCTTTTTCCTTTTTCAAATTCATATATATTGAACCTTCCTTTTTCAAATTCATATATATTGAACCATCAATATAAGATGTGAAACTACGATCTCTGTGGTATTCGAATCTAC

mRNA sequence

TGAATTTCTTCCATTCTTGGGAAAAGGAAAAAAAAGGAAAGAAAAAGAAAAATCTATTAAGTCATATTATATTATTATATTCGATAAGGAAATGTGAGAAATTCATCATCAGTCGTTGATTCTTAACGTGAGAGATTCGTGGACCGTCCGATGAGAATCTTGCATTCCCCAGTTTATAACGTTGGAGAAGCAACTTCGGCCACAAATTTGCTGCAAACCAACGCAAAACAGAACCCCAATTCTCACAGAGCAACAATGGCGGCAATGGTATCTCCATGGGGAAAGCCAGGCGCGTGGGCCCTCGACTCGGAGGAACACGAAGCTCAACTTCTGAAAGAAGAGCAGGAGCAGCAGAAACAAGCGGAGCTCGGCCTGGATTTCCCCTCCCTCAACGACGCCTTGGCCACCAAGCAGAAGAAGAAGGGCCAAACTCTCTCGTTGGCGGAGTTCAACGCCTATGGCGGCAGCGGCACCAGATCTTCCGCTCACACCGCTGCGCCCCAGGGCCTCACGCATGAAGATCTCATGGTGCTTCCCACCGGCCCTCGCCAACGGACGGCGGAGGAGCTCGACCGGAACCGCCTCGGCGGTGGCTTTAGGAATTACGGTACGAATGGTTCGTATGAGAGGAATAATAGGTATTCGATTGGGGATGATTCGTCTAATTCGAAGTGGGGTTCGTCTAGGGTTTCTGATGAGAATCGGAGGAACAATGGCGGGGGTAATAGGGGGGATTCGGGTAGGGAAATTGCACCTTCTCGGGCTGATGAGGTCGATAATTGGGCCTTAACTAAGAAACCGATGGCTGCAAATGGATTTGAGAGGAGGGATAGAGGAGGAGGGTTTTCGGATTTGCCGTCTTCGAAAGCCGACGAATCAGAGAGTTGGATTTCCAATAAGAGCAATGTTCCATCGGATGTGCGGAGATTTGGGACTAACGGCGGATTTGAAAGAGAGCGACGAGGAGGTTTTCCGACGGGCGGCGGAGGCGGTGCAGATTTAGAAAATTGGGGGAGGAAGAAAGAGGAAGGTAATAATGGTGGCAGCGTTGCGGGTAGGCCGAAGCTTAATTTGCAGCCACGGACACTGCCAGTGAGCGATGGGAAGTCGTCAGAGATATCAGGGACGGGGCTGGGGGCGAGGTCGAAGAGTTCAAATCCATTCGGAGAGGCGAGGCCGAGGGAACAGGTTCTGGCTGAGAAAGGACATGACTGGAAAAAACTCGACGAGCAACTGGAGTCGAAGATCAAAGACGAGCAACCAGAGAAGGTAGAAAACTCTAACAGCACTTCCTTTGGAAAGAAGAGCTTTGGCCGGGCATCTGAGGACCGAACAGAGAGGAGCTGGAGGAAGCCAGAGTCGACATCGGAGTCACTGGAATCTCGTCCCGGCAGTGCGGAGGAGAATGAAAATGGAGATGTTGAAGGGGAGTGTGAAGGGGACGAAAATGGAGCTTGATGGGATGAGATGATACAGAGAGATTGAAGTTAGTGAAAGTTTTGGAGTGATGAGTGATGACAACATTCTCTAATGTTGTTCTGATCCTTTATAATTTTGCCTCTGAGAAACAATTTTGATATGGTGGTGTTGGGGAGTTCTTGAGTCAGTTACTGTAATGTTTGAAGCCAACTCTTGGAATTAGAAACTACTACTGCTATTGTGCTACTCATCGGTTGAAAACAAAGGGCACCATCCATGGAGCAGGACGCAGAGTTGCCACTTTTAGCTCTGTTCCAAGGCATGCTAGGATGATGGGGTACCCGAGAACATATCTAGTGGAAATGGGGTGAAAAAGCTCTAGCATAGCTCTTTCTTTCTCGGGTCTCCTTTCAAAGGAAAAAGGCTGGTGCAGTTAGTAGCTAAAGGTTTCACTCAAACATGTGGTCTCGACGCGAAAAGAGCTTGCTTTCTACTTTCTACGTCTGCATTTTGTGTCCAGCCCGCAAAAGGGAAAATCGTGGAGAGATGTATCATGTCAGGATTCGTTGCTCAAATTGAATCCTTTCTTTATCATTGTTTCTATTCGTATTATTTGTAACTTGCCACTTGATTTGATTCGACTGAACATCTCGACCATTAACGAAAATTTCACCCACATCCGATAGGTTGGGCGCTCGACCAGCTTACAAGTTGGAGGAGGCTCCAACTATCCTTAAATTTTTTTTAAAATAACATAATAAACTCAAAGTATGATGTGTGTATATATAAAAAAAATGTTAAATTCTATATATTTTTAGTTAAATTAAATTCTCAACGGATTGTCCAAGAGACTAGACAAAGTCAACTGTCCAAAGCCTTTCCTTTACAAATCCCAACGGTCTCCCAGTCTCTGTACGCCGCCGCCGTCTCCGGCCACCGCGCCACTCTACGAAAATGCTCGTCTTCTTCCTCGCAGCCTTCTTCCTCTTCACGGCATCCAACTCCTCCCTCTTCCCCATCGACGAAGCCACCATCGCCGATATCCACAGCGCCTTCTCCCAAAACCACCTCACCTCCAGGCAACTCCTCGATCATTACCTCAACAAGATCGACCTCCTCAATCCAGTGCTTCGCAGCGTTCTGGAAGTGAATCCGGACGCCAGAGCTCAAGCGGAGGCCGCCGATCGAGAGCGGGACCTGGCCGGAGGAAAAGCCCTAGGCGAACTTCATGGAATCCCGGTGCTGCTCAAGGATTCCATCGCCACCAAGGGTCCGCTCAACACCACCGCCGGATCCTTCGCATTGCTCGGATCCGTGGTGCCCCGAGATGCGACGGTGGTCGCCCGCCTGAGGAAAGCCGGTGCGGTGATTTTGGGCAAAGCTTCGCTCACCGAGTGGTACGGATCTCGTTCTTTGAAGATTCCCAATGGCTGGTGCGCTCGTGGGGGTCAAGCCCTGAACCCATATGGGAAAGAAGGGGATCCATGTGGGTCAAGTAGTGGCTCTGCAATATCGGTGGCAGCCAACATGGCGGCAGTGTCGCTGGGAACTGAAACAGATGGCTCAATTCTATGTCCCGCAGATTACAACTCAGTCGTCGGCATCAAACCCACCGTCGGTCTCACGAGCCGGGCCGGCGTTATCCCGGTCACTCCTCGGCAAGACACCATCGGGCCTATATGCAGGACAGTTTCAGATGCAGTTTATTTGCTTGATGCCATAGTGGGGTTTGACCCAATGGACTCTGAAGCAACTAAAGCAGCATCTGAGTTCATTCCTTGTGGTGGCTACAAGCAATTTCTGAGGAAAGATGGGCTCAAAGGGAAGAGACTGGGCATTGTTAGGCATCCCTTTTTTGACTTGTATACCAACGGCTCCATGGCCATTCCAACCTTTGAACATCATGTCAATCTGCTGAGGAAAAGTGGAGCAACCATTGTGGATAACCTTCAAATATCAAATATAGATACAATTCTGGATCCTTATGGGAGTGGTGAACTTATTGCAACAATAGCTGAGTTCAAGCTCGCCATTAACGATTACCTCAAGAACCTGATCCACAGTCCAGTCAGATCTCTGGCTGACATTATTGCCTTCAACAACAACCACCCTGAACTGGAGAAGATGAACGAGTATGGGCAAGACGCCTTTCTTTTATCCGATCAAACAGATGGGATCGGCAAGGCGGAGGAAGAGGCAATTAGTATGATGGCAAACTTATCGAGAGATGGGTTCGAGTTGATGATGAAGACCTATAATCTGGATGCAATGGTAACTCTAGGGACATCAGCTGAATCCGTCCTAGCAATTGGGGGATACCCTGCAATTTCTGTTCCTGCTGGATATGAGGGAAACGGTGATCCATTTGGCATCCTCTTTGGGGGGCTCAGGGGAACCGAAACGAAGCTAATCGAGATCGCTTATGCATTCGAACAAGCGACCATGAACGGGTACAAGAAAACAGTACTTAAACCAGAAGAGATGAGTATAAAACTTCCATATGAAGCTCCTATATATCATGAAGACTTCCATTTAAGTTTTTCACCAACATGGCCTACACATCCCCGCTTAGTTCTGTAGTCTCGTCATTGCTTCTGATTCTTGTGGCCTTTTGTTTGACTGCCCCACAATCCACGATCGTTCAAGGGCTGTCGATCAGAGAAGCCACCGTGCATGACCTCCAGCTAGCTTTCAAGCAAAACCAGCTCACTTCGAGAGATCTTGTTGAGTTCTACCTAGACGAAACCCGTAGACTCAACCCGGTTGTCCATGGGATCATAGAAATCAACCCAGATGCATTGCTCCAAGCTGACAAGGCTGACCAAGAGCGCAAGGCTAAGAAGCCTGGATCGCTTTGTGGTCTGCATGGAATTCCAGTTCTACTGAAAGATAACATTGGGACTAAGGATAAGTTGAATACTACAGCTGGATCGTTCGCATTGCTTGGCTCTGTTGTGCCACATGATGCGTTCGTGGTAAAGAGACTGAGGAGGGCTGGAGCTATAATTCTGGGAAAGGCTAGCTTGAGTGAATGGGCCGATTTCCGATCTCTTACTGCTCCAGCGGGTTTGAGCGCCAGAGGTGGCCAGGGAAAGGTCAGTTACCTGCTCCAGAGACATTTTCCCCCACATCCAATGCTTTTCAAGTAGAATTAAAGACCATTTCTTTTGTACAATTCTCTTTTCAGAACCCATATATTTTATCAGCATCACCCTGTGGATCCAGCAGCGGGCCATCCATATCAGTTGCAACAAACTTAGCAGCAGTGTCAGTAGGAACCGAGACCGACGGTTCTATCCTCTGTCCAGCTAGTTCTAACTCGGTTGTTGGAATCAAACCAACAGTCGGCCTCACCAGTAGAGCAGGTGTCATTCCAGTTTCCCCTAGACAGGACACGATCGGGCCAATCTGCAGGACAGTGACGGATGCCGTAATAGTTCTTGACACCATTGTAGGGTTCGATTACAATGATGTAGCCTCCAGAACAGCCTCAAAGTACATTCCATATGGTGGCTATAAACAATTCCTCAATGCAAATGGGTTAAAGGGGAAGAGATTAGGCATTGTGAGGAGCCCTTTTTTCAGCTTCATCAATGATTCAACCATCACACAGGCTTTTGAGGATCATTTCAACACTCTAAGGTAACTGCCCTAAATCCTTAGCCAAATGGGTTTCTATTCATTTAAACAAAGAGTTGGAATCTATTGACAACTAGAAACCAATTCAATATGAACAACTATCTTCAAGTGGAATAGTTTACGTACCTCTAAAATTATCTTCTTCGGCTGTTCTTCTAGTAAACACTAAATCTAACACGTTGAAATTGAAGGCGAGGTGGTGCCATCTTGATAGACAATCTGGAGATAGCGAATATAGATACAATCTTGAACGCGACTGCAAGTGGGGAAGCAGTGGCGTTGCTCGCTGAATTCAAACAATCTTTGAATGCGTACCTGAAAGAGCTGGTGGCTTCCCCTGTTCGAAGTTTGGGAGACATAATCGCCTTCGATAACGCAGACCCAGATCTGGTAACATAACACTCAATCCATTTTTCTTCGCACAGTAACCGATTTCGTTCGTAATCGATTCGACCCATTGCTGTGCAGGAACTGCTCAAGGTTTTCGGCCAGGAGATCTTTCTGGCGGCGGAAGCGACAAATGGGATTGGCGACGTCCAGAAGGCGGCGCTGTTGAATTTGGCGAGGTTGACGAGAGATGGGTTTGAGAAATTGGTGAAGGAGAAGCGGTTGGACGCGGTGGTGACGCCGGGTTCCGGCATAGCTCCGGTGCTCGCGATTGGGGGTTTCCCCGGAATCAGTGTTCCGGCGGGATATGACGGCGGAGGAGTTCCGTTCGGGATTTGCTTCGGAGGGTTGAAGGGTTCGGAGGGGAAGCTGATTGAGGTCGCATATGGGTTTGAGCGAGCCACTCTGATTCGAAAGCCTCCTTCCTTCAAGCCTTGAACTTTGAAGCAAAGGCTATGAATCAGAATTACTCTTTGAATTCAAATTAATGGTTTTTTTTTTTCCTTTTTCCTTTTTCAAATTCATATATATTGAACCTTCCTTTTTCAAATTCATATATATTGAACCATCAATATAAGATGTGAAACTACGATCTCTGTGGTATTCGAATCTAC

Coding sequence (CDS)

ATGCTCGTCTTCTTCCTCGCAGCCTTCTTCCTCTTCACGGCATCCAACTCCTCCCTCTTCCCCATCGACGAAGCCACCATCGCCGATATCCACAGCGCCTTCTCCCAAAACCACCTCACCTCCAGGCAACTCCTCGATCATTACCTCAACAAGATCGACCTCCTCAATCCAGTGCTTCGCAGCGTTCTGGAAGTGAATCCGGACGCCAGAGCTCAAGCGGAGGCCGCCGATCGAGAGCGGGACCTGGCCGGAGGAAAAGCCCTAGGCGAACTTCATGGAATCCCGGTGCTGCTCAAGGATTCCATCGCCACCAAGGGTCCGCTCAACACCACCGCCGGATCCTTCGCATTGCTCGGATCCGTGGTGCCCCGAGATGCGACGGTGGTCGCCCGCCTGAGGAAAGCCGGTGCGGTGATTTTGGGCAAAGCTTCGCTCACCGAGTGGTACGGATCTCGTTCTTTGAAGATTCCCAATGGCTGGTGCGCTCGTGGGGGTCAAGCCCTGAACCCATATGGGAAAGAAGGGGATCCATGTGGGTCAAGTAGTGGCTCTGCAATATCGGTGGCAGCCAACATGGCGGCAGTGTCGCTGGGAACTGAAACAGATGGCTCAATTCTATGTCCCGCAGATTACAACTCAGTCGTCGGCATCAAACCCACCGTCGGTCTCACGAGCCGGGCCGGCGTTATCCCGGTCACTCCTCGGCAAGACACCATCGGGCCTATATGCAGGACAGTTTCAGATGCAGTTTATTTGCTTGATGCCATAGTGGGGTTTGACCCAATGGACTCTGAAGCAACTAAAGCAGCATCTGAGTTCATTCCTTGTGGTGGCTACAAGCAATTTCTGAGGAAAGATGGGCTCAAAGGGAAGAGACTGGGCATTGTTAGGCATCCCTTTTTTGACTTGTATACCAACGGCTCCATGGCCATTCCAACCTTTGAACATCATGTCAATCTGCTGAGGAAAAGTGGAGCAACCATTGTGGATAACCTTCAAATATCAAATATAGATACAATTCTGGATCCTTATGGGAGTGGTGAACTTATTGCAACAATAGCTGAGTTCAAGCTCGCCATTAACGATTACCTCAAGAACCTGATCCACAGTCCAGTCAGATCTCTGGCTGACATTATTGCCTTCAACAACAACCACCCTGAACTGGAGAAGATGAACGAGTATGGGCAAGACGCCTTTCTTTTATCCGATCAAACAGATGGGATCGGCAAGGCGGAGGAAGAGGCAATTAGTATGATGGCAAACTTATCGAGAGATGGGTTCGAGTTGATGATGAAGACCTATAATCTGGATGCAATGGTAACTCTAGGGACATCAGCTGAATCCGTCCTAGCAATTGGGGGATACCCTGCAATTTCTGTTCCTGCTGGATATGAGGGAAACGGTGATCCATTTGGCATCCTCTTTGGGGGGCTCAGGGGAACCGAAACGAAGCTAATCGAGATCGCTTATGCATTCGAACAAGCGACCATGAACGGGTACAAGAAAACAGTACTTAAACCAGAAGAGATGAGTATAAAACTTCCATATGAAGCTCCTATATATCATGAAGACTTCCATTTAAGTTTTTCACCAACATGGCCTACACATCCCCGCTTAGTTCTGTAG

Protein sequence

MLVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRSVLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSVVPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFFDLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAINDYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMANLSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGTETKLIEIAYAFEQATMNGYKKTVLKPEEMSIKLPYEAPIYHEDFHLSFSPTWPTHPRLVL
Homology
BLAST of MC04g0408 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 5.6e-159
Identity = 301/482 (62.45%), Postives = 362/482 (75.10%), Query Frame = 0

Query: 17  SSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRSVLEVNPDARAQAEAA 76
           SS F I EATI DI  AF++  LTS+QL++ YL  I  LNP+L +V+E NPDA  QAE A
Sbjct: 35  SSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAISKLNPILHAVIETNPDALIQAEIA 94

Query: 77  DRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSVVPRDATVVARLRKAG 136
           DRERDL     L  LHG+PVLLKDSI+TK  LNTTAGSFALLGSVV RDA VV RLR++G
Sbjct: 95  DRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTAGSFALLGSVVARDAGVVKRLRESG 154

Query: 137 AVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSSSGSAISVAANMAAVS 196
           AVILGKASL+EW   RS  IP+GW ARG Q  NPY    +P GSSSGSAISV AN+ AVS
Sbjct: 155 AVILGKASLSEWAHFRSFSIPDGWSARGLQGKNPYVLSANPSGSSSGSAISVTANLVAVS 214

Query: 197 LGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYLLDAI 256
           LGTETDGSIL PA  NSVVGIKP+VGLTSRAGV+P++ RQD+IGPICRTVSDAV+LLDAI
Sbjct: 215 LGTETDGSILSPASQNSVVGIKPSVGLTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAI 274

Query: 257 VGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIV-RHPFFDLYTNGSMAIPTFE 316
           VG+DP+D EATK ASEFIP GGYKQFL   GLKGKRLGIV +H                +
Sbjct: 275 VGYDPLD-EATKTASEFIPEGGYKQFLTTSGLKGKRLGIVMKHS------------SLLD 334

Query: 317 HHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAINDYLKNLIHSPVRSL 376
           HH+  LR+ GA +++NL I NI+ I+    SGE IA +AEFK+++N YLK L+ SPVRSL
Sbjct: 335 HHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKMSLNAYLKELVKSPVRSL 394

Query: 377 ADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMANLSRDGFELMMKTYN 436
           AD+IA+N    E EK+ E+GQ+ FL ++ T G+G+ E+ A+  M  LSR+G E +++   
Sbjct: 395 ADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENK 454

Query: 437 LDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGTETKLIEIAYAFEQA 496
           LDA+VTLG+   SVLAIGGYP I+VPAGY+  G P+GI FGGLR +E KLIEIA+AFEQA
Sbjct: 455 LDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQA 503

Query: 497 TM 498
           T+
Sbjct: 515 TL 503

BLAST of MC04g0408 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 7.8e-76
Identity = 193/501 (38.52%), Postives = 271/501 (54.09%), Query Frame = 0

Query: 22  IDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRSVLEVNPDARAQAEAADRERD 81
           +++ATI  + +      LTS  ++  YL++   +NP +  +L++NPD    A   D ER 
Sbjct: 66  LEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDER- 125

Query: 82  LAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSVVPRDATVVARLRKAGAVILG 141
            A G   G LHGIP ++KD+ ATK  ++TTAGS+ALLGS+VPRDA VV +LR+AGAV+ G
Sbjct: 126 -ANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVLFG 185

Query: 142 KASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSSSGSAISVAANMAAVSLGTET 201
            A+L+EW   RS     G+ ARGGQ+  P+    +P GSSSGSAISVA+NM A +LGTET
Sbjct: 186 HATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGTET 245

Query: 202 DGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYLLDAIVGFDP 261
           DGSI+ PA  N VVG+KPTVGLTSR GVIP +  QDT GPI RTV DAVY+  ++ G D 
Sbjct: 246 DGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGIDE 305

Query: 262 MDSEATKAASEFIPCGGYKQFL-RKDGLKGKRLGIVRHPFFDLYTNGSM-AIPTFEHHVN 321
            D        +    G Y +FL  K  L+G R G+   P+  L+ N     I      V 
Sbjct: 306 NDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGL---PWKRLWQNAKADEIDRLLEVVK 365

Query: 322 LLRKSGATIVDNLQISNIDTI--------LDPYGSGELIATIAEFKLAINDYLKNLIHSP 381
            + ++GA + +N    N+D I        L      E      +F   I  YL  + ++ 
Sbjct: 366 QIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKNTE 425

Query: 382 VRSLADIIAFNNNH-------PELEKMNEYGQDAFLLSDQTDGI-GKAEEEAISMMANLS 441
           + SL DI+ +NN +       P +      GQD FL S +  G+  +   +A+  +   S
Sbjct: 426 IHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRRTS 485

Query: 442 RD-GFELMMK--------TYNLDA-MVTLGTS-AESVLAIGGYPAISVPAGYEGNGDPFG 494
           +D G +  +         ++ L+  +V  GTS      A  GYP I++P G + NG PFG
Sbjct: 486 QDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGRPFG 545

BLAST of MC04g0408 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.3e-64
Identity = 167/490 (34.08%), Postives = 259/490 (52.86%), Query Frame = 0

Query: 30  IHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRSVLEVNPDARAQAEAADRERDLAGGKALG 89
           + + + Q ++    ++  Y+ +I  +N  +R+V E+NPDA   A+  D ER +  GK  G
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKM--GKLRG 153

Query: 90  ELHGIPVLLKDSIATKGPLNTTAGSFALLGSVVPRDATVVARLRKAGAVILGKASLTEWY 149
            LHG+P+++K++I T   +++TAGS+A+ G+    DATV  +LR+AG VI+GK+  ++W 
Sbjct: 154 PLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWA 213

Query: 150 GSRSLKIPNGWCARGGQALNPYGKEGDPCGSSSGSAISVAANMAAVSLGTETDGSILCPA 209
             RSL   NGW A GGQ    Y K  DP GSSSGS ++    +A  +LGTET GSI+ PA
Sbjct: 214 NFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPA 273

Query: 210 DYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYLLDAIVGFDPMDSEATKA 269
           D +++VG+KPTVGLTSR  V+P++ RQDT+GP+ R+V DA YLL  I G D  D+  +  
Sbjct: 274 DKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYTSAI 333

Query: 270 ASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFFDLYTNGSMAIPTFEHHVNLLRKSGATIV 329
             + IP   Y +    + LKGKR+G+ R+    ++ +    +  F   + +++K+GA IV
Sbjct: 334 PFDTIP--DYVKACDINALKGKRIGVPRN-VIKIFGSPQTVVDQFNQALAVMKKAGAIIV 393

Query: 330 DNLQISNIDTILDPYGSGELIATIAEFKLAINDYLKNLIHSP--VRSLADIIAFNNNHPE 389
           +N   ++           +++   A+    +  + K L  +P  +  L  +  F  +H  
Sbjct: 394 ENTDFTSFAEFAQSPIPDDIL--YADSLTNLPAFFKQLKVNPHNITDLESLRRFTQHH-- 453

Query: 390 LEKMNEYGQDAFLLSD--QTDGIGKAEEEAISMMANLSRDGFE----LMMKTYNLDAMVT 449
             ++ EY        D     GI   + +   M     + G E      ++ + LDA V 
Sbjct: 454 --RLEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVL 513

Query: 450 LGTSAESVLAIGGYPAISVPAGY---------------EGNGDPFGILFGGLRGTETKLI 497
               +  + A+ G P I+VP G                 G G P GI F G   +E KLI
Sbjct: 514 PTDLSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLI 572

BLAST of MC04g0408 vs. ExPASy Swiss-Prot
Match: B0JSX3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=gatA PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.3e-43
Identity = 156/490 (31.84%), Postives = 235/490 (47.96%), Query Frame = 0

Query: 26  TIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRSVLEVNPD-ARAQAEAADRERDLAG 85
           +I  +H        T+ ++   +L +I  L P ++S L + PD A AQA+  D +  +A 
Sbjct: 3   SIRQLHQQLVNKEKTAVEIATEFLARIQALEPQVKSFLHLTPDLALAQAQKVDEK--IAR 62

Query: 86  GKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSVVPRDATVVARLRKAGAVILGKAS 145
           G++L  L GIP+ LKD++ TKG + TT  S  L   V P ++TV  +LR  GAVI+GK +
Sbjct: 63  GESLHLLAGIPIALKDNLCTKG-IPTTCASRILENFVPPYESTVTQKLRDLGAVIVGKTN 122

Query: 146 LTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSSSGSAISVAANMAAVSLGTETDGS 205
           L E+    S +  +G+        NP+     P GSS GSA +VAA    V+LG++T GS
Sbjct: 123 LDEFAMGSSTE-NSGYHV----TANPWDLSRVPGGSSGGSAAAVAAQECVVALGSDTGGS 182

Query: 206 ILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYLLDAIVGFDPMDS 265
           I  PA +  VVG+KPT GL SR G++      D IGP  RTV DA  LL AI G+DP DS
Sbjct: 183 IRQPASFCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQAIAGYDPQDS 242

Query: 266 EATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFFDLYTNGSMAIPTFEHHVNLLRKS 325
            +       +P   Y QFL K  LKG ++G+++  F +      +        +  L+  
Sbjct: 243 TSLN-----LPIPDYSQFL-KTSLKGLKIGVIKETFGE--GLDQVVAEAVNQALAQLKAL 302

Query: 326 GATIVDNLQISNIDTILDPY---GSGELIATIAEF---KLAINDYLKNLIHSPVRSLADI 385
           GATI + +        L  Y      E  A +A +   K  I +   +LI    ++ A  
Sbjct: 303 GATIKE-ISCPRFRYGLPTYYIIAPSEASANLARYDGVKYGIREDADSLIDMYTKTRAK- 362

Query: 386 IAFNNNHPELEKMNEYG-----QDAFLLSDQ------TDGIGKAEEEAISMMANLS-RDG 445
             F         +  Y       DA+ L  Q       +   +A +    +++  S    
Sbjct: 363 -GFGAEVKRRIMLGTYTLSAGYYDAYYLKAQKVRTLIKEDFDRAFQSVDVLVSPTSPTTA 422

Query: 446 FELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGTETKLI 497
           F+   KT +  +M  L       + + G P +S+P G++G G P G+   G    E +L 
Sbjct: 423 FKAGEKTADPLSMY-LSDLMTIPVNLAGLPGLSLPCGFDGQGLPIGLQLVGNVLREDQLF 472

BLAST of MC04g0408 vs. ExPASy Swiss-Prot
Match: Q8DK65 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=gatA PE=3 SV=2)

HSP 1 Score: 174.9 bits (442), Expect = 2.5e-42
Identity = 158/497 (31.79%), Postives = 236/497 (47.48%), Query Frame = 0

Query: 25  ATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRSVLEVNPDARAQAEAADRERDLAG 84
           + I ++H    +   ++ ++   YL++I  + P L S L V  D RA A+AA+ ++ LA 
Sbjct: 2   SVIQELHRQLVRKERSATEITQAYLDRIAQVEPTLHSFLTVTRD-RALAQAAEVDKRLAA 61

Query: 85  GKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSVVPRDATVVARLRKAGAVILGKAS 144
           G+ +G L GIP+ +KD++ T G + TT  S  L   V P ++TV  +L+ AGA+++GK +
Sbjct: 62  GEEIGLLTGIPLAIKDNLCTYG-VRTTCASKMLEHFVPPYESTVTEKLQAAGAIMVGKTN 121

Query: 145 LTEW-YGSRSLKIPNGWCARGGQALNPYGKEGDPCGSSSGSAISVAANMAAVSLGTETDG 204
           L E+  GS +     G+ A      NP+  E    GSS GSA +VAA   A +LG++T G
Sbjct: 122 LDEFAMGSSTENSAFGFTA------NPWNPERVSGGSSGGSAAAVAARECAAALGSDTGG 181

Query: 205 SILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYLLDAIVGFDPMD 264
           SI  PA +  VVG+KPT GL SR G++      D IGP+  TV+DA  LL AI G DP D
Sbjct: 182 SIRQPAAFCGVVGLKPTYGLVSRYGLVAYASSLDQIGPLAPTVTDAAILLGAIAGHDPKD 241

Query: 265 SEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFFDLYTNGSMAIP----TFEHHVN 324
                A S  +P   Y Q L+ D LKG R+G+++       T G    P      E  + 
Sbjct: 242 -----ATSLRVPIPDYTQALKPD-LKGMRIGLIQE------TVGEGVQPEVKSALEAALK 301

Query: 325 LLRKSGATIVDNLQISNIDTILDPY---GSGELIATIAEFKLAIN-----DYLKNLIHSP 384
            L   GAT+V+ L        L  Y      E  A +A +   +N     +   +L+   
Sbjct: 302 TLEALGATLVE-LSCPRFAYGLPTYYIIAPSEASANLARYD-GVNFGFRAEGASDLLEMY 361

Query: 385 VRSLADIIAFNNNHPELEKMNEYG-----QDAFLLSDQ-------TDGIGKAEEEAISMM 444
           +++ A    F         +  Y       DA+ L  Q        D     E+  + + 
Sbjct: 362 MKTRAQ--GFGAEVKRRIMIGTYALSAGYYDAYYLRAQKVRTLIKEDFARAFEQVDVLIC 421

Query: 445 ANLSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLR 497
                  F+   KT +  +M  L       + + G P +SVP G++ NG P G+   G  
Sbjct: 422 PTAPTTAFKAGEKTADPLSMY-LSDLMTIPVNLAGLPGLSVPCGFDSNGLPIGLQLIGNV 473

BLAST of MC04g0408 vs. NCBI nr
Match: XP_022136311.1 (putative amidase C869.01 [Momordica charantia])

HSP 1 Score: 970 bits (2508), Expect = 0.0
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MLVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLR 60
           MLVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLR
Sbjct: 1   MLVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLR 60

Query: 61  SVLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGS 120
           SVLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGS
Sbjct: 61  SVLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGS 120

Query: 121 VVPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGS 180
           VVPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGS
Sbjct: 121 VVPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGS 180

Query: 181 SSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIG 240
           SSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIG
Sbjct: 181 SSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIG 240

Query: 241 PICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPF 300
           PICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPF
Sbjct: 241 PICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPF 300

Query: 301 FDLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAI 360
           FDLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAI
Sbjct: 301 FDLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAI 360

Query: 361 NDYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMA 420
           NDYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMA
Sbjct: 361 NDYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMA 420

Query: 421 NLSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRG 480
           NLSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRG
Sbjct: 421 NLSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRG 480

Query: 481 TETKLIEIAYAFEQATM 497
           TETKLIEIAYAFEQATM
Sbjct: 481 TETKLIEIAYAFEQATM 497

BLAST of MC04g0408 vs. NCBI nr
Match: XP_023529660.1 (probable amidase At4g34880 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 848 bits (2191), Expect = 3.32e-306
Identity = 432/496 (87.10%), Postives = 458/496 (92.34%), Query Frame = 0

Query: 2   LVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRS 61
           +VFFL+AF LF  +NSS FPIDEATIA+I  AFSQN LTSR+LLDHYLNKID LNPVLRS
Sbjct: 45  IVFFLSAFLLFIGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRS 104

Query: 62  VLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSV 121
           VLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSI TK  LNTTAGSFALLGSV
Sbjct: 105 VLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSV 164

Query: 122 VPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSS 181
           VPRDATVV RLR AGAVILGK SLTEWYGSRSLKIP+GWCARGGQALNPYGK GDPCGSS
Sbjct: 165 VPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSS 224

Query: 182 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGP 241
           SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++PRQDTIGP
Sbjct: 225 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGP 284

Query: 242 ICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFF 301
           ICRTVSDAVY+LDAIVGFDPMDSEATK  S+FIP GGYKQFL++DGL GKRLGIVRHPF 
Sbjct: 285 ICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFS 344

Query: 302 DLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAIN 361
           DLY NGS AI +FEHHV LLRKSGATIVDNLQISN+  I +PY SGEL+A IAEFKL IN
Sbjct: 345 DLYANGSTAIQSFEHHVKLLRKSGATIVDNLQISNVAVIQNPYESGELVAMIAEFKLTIN 404

Query: 362 DYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMAN 421
           DYLKNLI SPVRSLADIIAFNNNHPELE M EYGQDAFLLS+QT+GIG+AE+ AISMM+N
Sbjct: 405 DYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSN 464

Query: 422 LSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGT 481
           LSRDGFE MMKTYNLDAMVTLGT AE+VLAIGGYPAISVPAGYEGNG+PFG+LF GL+GT
Sbjct: 465 LSRDGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGT 524

Query: 482 ETKLIEIAYAFEQATM 497
           E KLIEIAYA+EQATM
Sbjct: 525 EPKLIEIAYAYEQATM 540

BLAST of MC04g0408 vs. NCBI nr
Match: XP_022928333.1 (putative amidase C869.01 [Cucurbita moschata])

HSP 1 Score: 848 bits (2190), Expect = 3.63e-306
Identity = 431/496 (86.90%), Postives = 457/496 (92.14%), Query Frame = 0

Query: 2   LVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRS 61
           +VFFL+ F LF  +NSS FPIDEATIA+I  AFSQN LTSR+LLDHYLNKID LNPVLRS
Sbjct: 38  IVFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRS 97

Query: 62  VLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSV 121
           VLEVNPDARAQAE ADRER+LAGGKALGELHGIPVLLKDSI TK  LNTTAGSFALLGSV
Sbjct: 98  VLEVNPDARAQAETADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSV 157

Query: 122 VPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSS 181
           VPRDATVV RLR AGAVILGK SLTEWYGSRSLKIP+GWCARGGQALNPYGK GDPCGSS
Sbjct: 158 VPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSS 217

Query: 182 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGP 241
           SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++PRQDTIGP
Sbjct: 218 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGP 277

Query: 242 ICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFF 301
           ICRTVSDAVY+LDAIVGFDPMDSEATK  S+FIP GGYKQFL++DGL GKRLGIVRHPF 
Sbjct: 278 ICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFS 337

Query: 302 DLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAIN 361
           DLY NGS AI TFEHHV LLRKSGATIVDNLQISN+  IL+PY SGEL+A IAEFKL IN
Sbjct: 338 DLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLTIN 397

Query: 362 DYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMAN 421
           DYLKNLI SPVRSLADIIAFNNNHPELE M EYGQDAFLLS+QT+GIG+AE+ AISMM+N
Sbjct: 398 DYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSN 457

Query: 422 LSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGT 481
           LSRDGFE MMKTYNLDAMVTLGT AE+VLAIGGYPAISVPAGYEGNG+PFG+LF GL+GT
Sbjct: 458 LSRDGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGT 517

Query: 482 ETKLIEIAYAFEQATM 497
           E KLIEIAYA+EQATM
Sbjct: 518 EPKLIEIAYAYEQATM 533

BLAST of MC04g0408 vs. NCBI nr
Match: KAG7015266.1 (hypothetical protein SDJN02_22900 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 846 bits (2186), Expect = 1.47e-305
Identity = 429/496 (86.49%), Postives = 458/496 (92.34%), Query Frame = 0

Query: 2   LVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRS 61
           ++FFL+ F LF  +NSS FPIDEATIA+I  AFSQN LTSR+LLDHYLNKID LNPVLRS
Sbjct: 38  IIFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRS 97

Query: 62  VLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSV 121
           VLEVNPDARAQAEAADRER+LAGGKALGELHGIPVLLKDSI TK  LNTTAGSFALLGSV
Sbjct: 98  VLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSV 157

Query: 122 VPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSS 181
           VPRDATVV RLR AGAVILGK SLTEWYGSRSL+IP+GWCARGGQALNPYGK GDPCGSS
Sbjct: 158 VPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSS 217

Query: 182 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGP 241
           SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++PRQDTIGP
Sbjct: 218 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGP 277

Query: 242 ICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFF 301
           ICRTVSDAVY+LDAIVGFDPMDSEATK  S+FIP GGYKQFL++DGL GKRLGIVRHPF 
Sbjct: 278 ICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFS 337

Query: 302 DLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAIN 361
           DLY NGS AI TFEHHV LLRKSGATIVDNLQISN+  IL+PY SGEL+A IAEFKL IN
Sbjct: 338 DLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLTIN 397

Query: 362 DYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMAN 421
           DYLKNLI SPVRSLADIIAFNNNHPELE M EYGQDAFLLS+QT+GIG+AE+ AISMM+N
Sbjct: 398 DYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSN 457

Query: 422 LSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGT 481
           LSRDGFE MMKTYNLD+MVTLGT AE+VLAIGGYPAISVPAGYEGNG+PFG+LF GL+GT
Sbjct: 458 LSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGT 517

Query: 482 ETKLIEIAYAFEQATM 497
           E KLIEIAYA+EQATM
Sbjct: 518 EPKLIEIAYAYEQATM 533

BLAST of MC04g0408 vs. NCBI nr
Match: KAG6589001.1 (putative amidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 845 bits (2182), Expect = 5.99e-305
Identity = 428/496 (86.29%), Postives = 457/496 (92.14%), Query Frame = 0

Query: 2   LVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRS 61
           ++FFL+ F LF  +NSS FPIDEATIA+I  AFSQN LTSR+LLDHYLNKID LNPVLRS
Sbjct: 38  IIFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRS 97

Query: 62  VLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSV 121
           VLEVNPDARAQAEAADRER+LAGGK LGELHGIPVLLKDSI TK  LNTTAGSFALLGSV
Sbjct: 98  VLEVNPDARAQAEAADRERELAGGKGLGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSV 157

Query: 122 VPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSS 181
           VPRDATVV RLR AGAVILGK SLTEWYGSRSL+IP+GWCARGGQALNPYGK GDPCGSS
Sbjct: 158 VPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKGGDPCGSS 217

Query: 182 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGP 241
           SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++PRQDTIGP
Sbjct: 218 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGP 277

Query: 242 ICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFF 301
           ICRTVSDAVY+LDAIVGFDPMDSEATK  S+FIP GGYKQFL++DGL GKRLGIVRHPF 
Sbjct: 278 ICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFS 337

Query: 302 DLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAIN 361
           DLY NGS AI TFEHHV LLRKSGATIVDNLQISN+  IL+PY SGEL+A IAEFKL IN
Sbjct: 338 DLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLTIN 397

Query: 362 DYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMAN 421
           DYLKNLI SPVRSLADIIAFNNNHPELE M EYGQDAFLLS+QT+GIG+AE+ AISMM+N
Sbjct: 398 DYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSN 457

Query: 422 LSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGT 481
           LSRDGFE MMKTYNLD+MVTLGT AE+VLAIGGYPAISVPAGYEGNG+PFG+LF GL+GT
Sbjct: 458 LSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGT 517

Query: 482 ETKLIEIAYAFEQATM 497
           E KLIEIAYA+EQATM
Sbjct: 518 EPKLIEIAYAYEQATM 533

BLAST of MC04g0408 vs. ExPASy TrEMBL
Match: A0A6J1C363 (putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111008028 PE=4 SV=1)

HSP 1 Score: 970 bits (2508), Expect = 0.0
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MLVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLR 60
           MLVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLR
Sbjct: 1   MLVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLR 60

Query: 61  SVLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGS 120
           SVLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGS
Sbjct: 61  SVLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGS 120

Query: 121 VVPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGS 180
           VVPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGS
Sbjct: 121 VVPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGS 180

Query: 181 SSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIG 240
           SSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIG
Sbjct: 181 SSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIG 240

Query: 241 PICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPF 300
           PICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPF
Sbjct: 241 PICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPF 300

Query: 301 FDLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAI 360
           FDLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAI
Sbjct: 301 FDLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAI 360

Query: 361 NDYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMA 420
           NDYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMA
Sbjct: 361 NDYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMA 420

Query: 421 NLSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRG 480
           NLSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRG
Sbjct: 421 NLSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRG 480

Query: 481 TETKLIEIAYAFEQATM 497
           TETKLIEIAYAFEQATM
Sbjct: 481 TETKLIEIAYAFEQATM 497

BLAST of MC04g0408 vs. ExPASy TrEMBL
Match: A0A6J1ENQ3 (putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111435192 PE=4 SV=1)

HSP 1 Score: 848 bits (2190), Expect = 1.76e-306
Identity = 431/496 (86.90%), Postives = 457/496 (92.14%), Query Frame = 0

Query: 2   LVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRS 61
           +VFFL+ F LF  +NSS FPIDEATIA+I  AFSQN LTSR+LLDHYLNKID LNPVLRS
Sbjct: 38  IVFFLSVFLLFVGANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLRS 97

Query: 62  VLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSV 121
           VLEVNPDARAQAE ADRER+LAGGKALGELHGIPVLLKDSI TK  LNTTAGSFALLGSV
Sbjct: 98  VLEVNPDARAQAETADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSV 157

Query: 122 VPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSS 181
           VPRDATVV RLR AGAVILGK SLTEWYGSRSLKIP+GWCARGGQALNPYGK GDPCGSS
Sbjct: 158 VPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKGGDPCGSS 217

Query: 182 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGP 241
           SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++PRQDTIGP
Sbjct: 218 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGP 277

Query: 242 ICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFF 301
           ICRTVSDAVY+LDAIVGFDPMDSEATK  S+FIP GGYKQFL++DGL GKRLGIVRHPF 
Sbjct: 278 ICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFS 337

Query: 302 DLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAIN 361
           DLY NGS AI TFEHHV LLRKSGATIVDNLQISN+  IL+PY SGEL+A IAEFKL IN
Sbjct: 338 DLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLTIN 397

Query: 362 DYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMAN 421
           DYLKNLI SPVRSLADIIAFNNNHPELE M EYGQDAFLLS+QT+GIG+AE+ AISMM+N
Sbjct: 398 DYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMSN 457

Query: 422 LSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGT 481
           LSRDGFE MMKTYNLDAMVTLGT AE+VLAIGGYPAISVPAGYEGNG+PFG+LF GL+GT
Sbjct: 458 LSRDGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVLFSGLKGT 517

Query: 482 ETKLIEIAYAFEQATM 497
           E KLIEIAYA+EQATM
Sbjct: 518 EPKLIEIAYAYEQATM 533

BLAST of MC04g0408 vs. ExPASy TrEMBL
Match: A0A6J1JLA2 (putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111486234 PE=4 SV=1)

HSP 1 Score: 837 bits (2161), Expect = 1.23e-302
Identity = 426/496 (85.89%), Postives = 454/496 (91.53%), Query Frame = 0

Query: 2   LVFFLAAFFLFTASNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRS 61
           +VFFL+AF  F  +NSS FPIDEAT+A+I  AFSQN LTSR+LLDHYLNKID LNPVL+S
Sbjct: 3   IVFFLSAFLFFIGANSSQFPIDEATVAEIQHAFSQNKLTSRELLDHYLNKIDFLNPVLKS 62

Query: 62  VLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSV 121
           VLEVNPDARAQAEAADRER+LAGGKALGELHGIPVLLKDSI TK  LNTTAGSFALLGSV
Sbjct: 63  VLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGSFALLGSV 122

Query: 122 VPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSS 181
           VPRDA VV RLR AGAVILGK SLTEWYGSRS+KIP+GWCARGGQALNPYGK GDPCGSS
Sbjct: 123 VPRDAKVVHRLRNAGAVILGKTSLTEWYGSRSMKIPHGWCARGGQALNPYGKGGDPCGSS 182

Query: 182 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGP 241
           SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++PRQDTIGP
Sbjct: 183 SGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGP 242

Query: 242 ICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFF 301
           ICRTVSDAVY+LDAIVGFDPMDSEATK  S+FIP GGYKQFL++DGL GKRLGIVRHPF 
Sbjct: 243 ICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLGIVRHPFS 302

Query: 302 DLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAIN 361
           DLY N S AI +FEHHV LLRKSGATIVDNLQISN+  IL+PY SGEL+A IAEFKL IN
Sbjct: 303 DLYANESTAIQSFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIAEFKLTIN 362

Query: 362 DYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMAN 421
           DYLKNLI SPVRSLADIIAFNNNHPELE M EYGQDAFLLS+QT+GIG+AE+ AISMM N
Sbjct: 363 DYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKAAISMMEN 422

Query: 422 LSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGT 481
           LSR GFE MMKTYNLDAMVTLGT AE+VLAIGGYPAISVPAGYEGNG+PFGILF GL+GT
Sbjct: 423 LSRHGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGILFSGLKGT 482

Query: 482 ETKLIEIAYAFEQATM 497
           E KLIEIAYA+EQATM
Sbjct: 483 EPKLIEIAYAYEQATM 498

BLAST of MC04g0408 vs. ExPASy TrEMBL
Match: A0A1S3BIN0 (putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103490302 PE=4 SV=1)

HSP 1 Score: 799 bits (2064), Expect = 2.43e-287
Identity = 411/495 (83.03%), Postives = 446/495 (90.10%), Query Frame = 0

Query: 4   FFLAAFFLFTA-SNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRSV 63
           FFL+A  LFT  +NSS F IDEATI +I +AFSQN LTS QLLD+YLNKI LLNPVL+SV
Sbjct: 38  FFLSAVLLFTGLANSSYFSIDEATIVEIQNAFSQNKLTSTQLLDYYLNKIHLLNPVLKSV 97

Query: 64  LEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSVV 123
           LE+NPDARAQAEAADRER LA GK+LGELHGIP+LLKD+IATK  LNTTAGSFALLGSVV
Sbjct: 98  LELNPDARAQAEAADRERLLACGKSLGELHGIPILLKDAIATKDLLNTTAGSFALLGSVV 157

Query: 124 PRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSSS 183
           PRDATVV+RLR AGAVILGK SLTEW+ SRS KIPNGWCARGGQA+NPYGK GDPCGSSS
Sbjct: 158 PRDATVVSRLRNAGAVILGKTSLTEWFKSRSSKIPNGWCARGGQAVNPYGKGGDPCGSSS 217

Query: 184 GSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPI 243
           GSAISVAANM AVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++PRQDTIGPI
Sbjct: 218 GSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISPRQDTIGPI 277

Query: 244 CRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPFFD 303
           CRTVSDAVY+L+AIVGFDPMD E TK AS+FIP GGYKQFLRKDGLKGKRLGIVRHPF D
Sbjct: 278 CRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRKDGLKGKRLGIVRHPFSD 337

Query: 304 LYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAIND 363
           LY+N SMAIPTFE H+NLLRK GATIVDNLQISN+D IL+PY SGE IA IAEFKLA+ND
Sbjct: 338 LYSNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNPYESGEFIAIIAEFKLAVND 397

Query: 364 YLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMANL 423
           YLK LI SPVRSLADII+FNN HPELE M EYGQDAFLLS+QT+GIG+ E+EAIS M NL
Sbjct: 398 YLKKLIQSPVRSLADIISFNNKHPELENMKEYGQDAFLLSEQTNGIGEMEKEAISRMMNL 457

Query: 424 SRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGTE 483
           SR+GFE +MK  NLDAMVT+GT  ESVLAIGGYP ISVPAGYE NG+PFGILFGGL+G+E
Sbjct: 458 SRNGFEELMKGNNLDAMVTIGTGIESVLAIGGYPGISVPAGYEENGEPFGILFGGLKGSE 517

Query: 484 TKLIEIAYAFEQATM 497
            KLIEIAYA+EQATM
Sbjct: 518 PKLIEIAYAYEQATM 532

BLAST of MC04g0408 vs. ExPASy TrEMBL
Match: A0A0A0K188 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009770 PE=4 SV=1)

HSP 1 Score: 795 bits (2053), Expect = 3.51e-286
Identity = 411/497 (82.70%), Postives = 446/497 (89.74%), Query Frame = 0

Query: 2   LVFFLAAFFLFTA-SNSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLR 61
           +VFFL+A  LFT  +NSS F IDEATIA+I +AFSQN LTS QLLD+YL KI LLNPVL+
Sbjct: 3   IVFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHLLNPVLK 62

Query: 62  SVLEVNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGS 121
           SVLE+NPDARAQAEAADRER LAGGKA GELHG+P+LLKD+IATK  LNTTAGSFALLGS
Sbjct: 63  SVLELNPDARAQAEAADRERLLAGGKARGELHGVPILLKDAIATKDLLNTTAGSFALLGS 122

Query: 122 VVPRDATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGS 181
           VVPRDATVV+RLR AGAVILGK SLTEWY SRS +IPNGWCARGGQA+NPYG+ GDPCGS
Sbjct: 123 VVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSFEIPNGWCARGGQAVNPYGRGGDPCGS 182

Query: 182 SSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIG 241
           SSGSAISVAANM AVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIG
Sbjct: 183 SSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIG 242

Query: 242 PICRTVSDAVYLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIVRHPF 301
           PICRTVSDAVY+L+AIVGFDPMD E TK AS+FIP GGYKQFLRK+GLKGKRLGIVRHPF
Sbjct: 243 PICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRKNGLKGKRLGIVRHPF 302

Query: 302 FDLYTNGSMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAI 361
            DLY N S+AIPTFE H+NLLRK GATIVDNLQISN+D IL+ Y SGE IA IAEFK+AI
Sbjct: 303 SDLYPNNSIAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIAEFKVAI 362

Query: 362 NDYLKNLIHSPVRSLADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMA 421
           NDYLK LI SPVRSLADII+FNNNH ELEKM EYGQDAFLLS+QT+GIG  E+EAIS MA
Sbjct: 363 NDYLKKLIRSPVRSLADIISFNNNHAELEKMKEYGQDAFLLSEQTNGIGVMEKEAISKMA 422

Query: 422 NLSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRG 481
           NLSR+GFE +MK  NLDAMVT+G   ESVLAIGGYP ISVPAGYE NG+PFGILFGGL+G
Sbjct: 423 NLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGILFGGLKG 482

Query: 482 TETKLIEIAYAFEQATM 497
           +E KLIEIAYA+EQATM
Sbjct: 483 SEPKLIEIAYAYEQATM 499

BLAST of MC04g0408 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 483.0 bits (1242), Expect = 3.1e-136
Identity = 267/482 (55.39%), Postives = 326/482 (67.63%), Query Frame = 0

Query: 17  SSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRSVLEVNPDARAQAEAA 76
           SS F I EATI DI  AF++  LTS+QL++ YL  I  LNP+L +V+E NPDA  QAE A
Sbjct: 35  SSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAISKLNPILHAVIETNPDALIQAEIA 94

Query: 77  DRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSVVPRDATVVARLRKAG 136
           DRERDL     L  LHG+PVLLKDSI+TK  LNTTAGSFALLGSVV RDA VV RLR++G
Sbjct: 95  DRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTAGSFALLGSVVARDAGVVKRLRESG 154

Query: 137 AVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSSSGSAISVAANMAAVS 196
           AVILGKASL+EW   RS  IP+GW A                                  
Sbjct: 155 AVILGKASLSEWAHFRSFSIPDGWSA---------------------------------- 214

Query: 197 LGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYLLDAI 256
                          NSVVGIKP+VGLTSRAGV+P++ RQD+IGPICRTVSDAV+LLDAI
Sbjct: 215 ------------PSQNSVVGIKPSVGLTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAI 274

Query: 257 VGFDPMDSEATKAASEFIPCGGYKQFLRKDGLKGKRLGIV-RHPFFDLYTNGSMAIPTFE 316
           VG+DP+D EATK ASEFIP GGYKQFL   GLKGKRLGIV +H                +
Sbjct: 275 VGYDPLD-EATKTASEFIPEGGYKQFLTTSGLKGKRLGIVMKHS------------SLLD 334

Query: 317 HHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAINDYLKNLIHSPVRSL 376
           HH+  LR+ GA +++NL I NI+ I+    SGE IA +AEFK+++N YLK L+ SPVRSL
Sbjct: 335 HHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKMSLNAYLKELVKSPVRSL 394

Query: 377 ADIIAFNNNHPELEKMNEYGQDAFLLSDQTDGIGKAEEEAISMMANLSRDGFELMMKTYN 436
           AD+IA+N    E EK+ E+GQ+ FL ++ T G+G+ E+ A+  M  LSR+G E +++   
Sbjct: 395 ADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENK 454

Query: 437 LDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGGLRGTETKLIEIAYAFEQA 496
           LDA+VTLG+   SVLAIGGYP I+VPAGY+  G P+GI FGGLR +E KLIEIA+AFEQA
Sbjct: 455 LDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQA 457

Query: 497 TM 498
           T+
Sbjct: 515 TL 457

BLAST of MC04g0408 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 130.2 bits (326), Expect = 5.1e-30
Identity = 145/525 (27.62%), Postives = 235/525 (44.76%), Query Frame = 0

Query: 16  NSSLFPIDEATIADIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRSVLEVNPDARAQAEA 75
           ++S+    ++ I     +      T+ ++   YL++I L  P L+  L V+ +    A+ 
Sbjct: 35  DTSVISPPQSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQE 94

Query: 76  ADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSVVPRDATVVARLRKA 135
            D+   +A G+ LG L G+ + +KD+I T+G + +TA S  L     P DAT V ++++ 
Sbjct: 95  IDQR--IAKGEELGPLAGVLIGVKDNICTQG-MPSTAASRILEHYRPPFDATAVKKIKEL 154

Query: 136 GAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSSSGSAISVAANMAAV 195
           G +++GK ++ E +G  S    + +        NP+     P GSS GSA +VAA    V
Sbjct: 155 GGIVVGKTNMDE-FGMGSTTEASAFQV----TANPWDLSRVPGGSSGGSAAAVAARQCMV 214

Query: 196 SLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYLLDA 255
           SLG++T GS+  PA +  VVG+KPT G  SR G++      D IG    TV+DA  LL A
Sbjct: 215 SLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHA 274

Query: 256 IVGFDPMDSEATKA-ASEFIPCGGYKQFLRKD-----GLKGKRLGIVRHPFFDLYTNGSM 315
           I G+D  DS ++K    EF       QFL  D      L G ++GI+R    D   +G  
Sbjct: 275 ISGYDRFDSTSSKQDVPEF-----QSQFLSVDHFESKPLNGVKVGIIRETLEDGVDSG-- 334

Query: 316 AIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPYGSGELIATIAEFKLAINDYLKNLIH 375
                   V    +  A+ ++ L     +  L  +  G      A + +A ++   NL  
Sbjct: 335 --------VRSATQEAASHLEALGCILTEVSLPSFSLG----LPAYYVIASSESSSNL-- 394

Query: 376 SPVRSLADIIAFNNN--HPELEKMNE------YGQDA---FLLSDQ--TDGIGKAEEEAI 435
               S  D + + N     EL K+ E      +G +     L+     + G   A  +  
Sbjct: 395 ----SRYDGVRYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGTYALSAGYYDAYYKRA 454

Query: 436 SMMANLSRDGFELMMKTYNL-------DAMVTLGTSAESVLA------------IGGYPA 495
             +  L R  F+  ++  ++        A   +G   +  LA            + G PA
Sbjct: 455 QQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDIMTVNVNLAGLPA 514

Query: 496 ISVPAG-YEG--NGDPFGILFGGLRGTETKLIEIAYAFEQATMNG 500
           + +P G  EG  +G P G+   G    E KL+++ + FEQ T+ G
Sbjct: 515 MVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQ-TLKG 525

BLAST of MC04g0408 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 122.5 bits (306), Expect = 1.1e-27
Identity = 88/268 (32.84%), Postives = 137/268 (51.12%), Query Frame = 0

Query: 10  FLFTASNSSLFPIDEATIA-----DIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRSVLE 69
           F + +++    P DE  IA     ++        +TS++L+  YL ++   N VL +V+ 
Sbjct: 183 FKYPSASGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVT 242

Query: 70  VNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSVVPR 129
              +  A  +A + +  L+ G  LG LHGIP  LKD +A  G   TT GS +     +  
Sbjct: 243 YTEEL-AYKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDI 302

Query: 130 DATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSSSGS 189
           +A V  RL+ +GAV++ K        + S+   + W   GG+  NP+  E    GSS+G 
Sbjct: 303 EAWVYKRLKASGAVLVAKLV------TGSMAYDDIWF--GGRTRNPWNIEEFSTGSSAGP 362

Query: 190 AISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICR 249
           A S +A M   ++G+ET GS+  PA    +  ++PT G   R GV+ ++   D +GP CR
Sbjct: 363 AASTSAGMVPFAIGSETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCR 422

Query: 250 TVSDAVYLLDAIVGFDPMDSEATKAASE 273
           T +D   +LDAI G DP D  + + A E
Sbjct: 423 TAADCAVILDAIKGKDPDDLSSREIAFE 440

BLAST of MC04g0408 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 113.6 bits (283), Expect = 4.9e-25
Identity = 87/268 (32.46%), Postives = 134/268 (50.00%), Query Frame = 0

Query: 10  FLFTASNSSLFPIDEATIA-----DIHSAFSQNHLTSRQLLDHYLNKIDLLNPVLRSVLE 69
           F + +++    P DE  IA     ++        +TS++L+  YL ++   N VL +V+ 
Sbjct: 183 FKYPSASGVKLPRDEEDIAFMSVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVT 242

Query: 70  VNPDARAQAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFALLGSVVPR 129
              +  A  +A + +  L+ G  LG LHGIP  LKD +A  G   TT GS +     +  
Sbjct: 243 YTEEL-AYKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDI 302

Query: 130 DATVVARLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSSSGS 189
           +A V  RL+ +GAV++ K        + S+   + W   GG+  NP+  E    GSS+G 
Sbjct: 303 EAWVYKRLKASGAVLVAKLV------TGSMAYDDIWF--GGRTRNPWNIEEFSTGSSAGP 362

Query: 190 AISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICR 249
           A       A+ S G+ET GS+  PA    +  ++PT G   R GV+ ++   D +GP CR
Sbjct: 363 A-------ASTSAGSETAGSMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCR 422

Query: 250 TVSDAVYLLDAIVGFDPMDSEATKAASE 273
           T +D   +LDAI G DP D  + + A E
Sbjct: 423 TAADCAVILDAIKGKDPDDLSSREIAFE 433

BLAST of MC04g0408 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 85.9 bits (211), Expect = 1.1e-16
Identity = 118/437 (27.00%), Postives = 189/437 (43.25%), Query Frame = 0

Query: 72  QAEAADRERDLAGGKALGELHGIPVLLKDSIATKGPLNTTAGSFAL-LGSVVPRDATVVA 131
           QAEA+ R      G  +  L GI V +KD I    P  T  G+  L     V +D+ VV+
Sbjct: 180 QAEASTRR--FEQGNPISVLDGIFVTIKDDIDCL-PHPTNGGTTWLHEDRSVEKDSAVVS 239

Query: 132 RLRKAGAVILGKASLTEWYGSRSLKIPNGWCARGGQALNPYGKEGDPCGSSSGSAISVAA 191
           +LR  GA++LGKA++ E  G  +     G  +  G   NP+  +    GSSSGSA  VAA
Sbjct: 240 KLRSCGAILLGKANMHE-LGMGT----TGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAA 299

Query: 192 NMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAV 251
            + + +LGT+  GS+  P+    + G+K T G T   G +      + IGP+  ++ DA 
Sbjct: 300 GLCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAF 359

Query: 252 YLLDAIVGFDPMDSEATKAASEFIPCGGYKQFLRKDG---LKGKRLGIVRHPFFDLYTNG 311
            +  AI+G    D    K +    PC  + + L  +G   +   RLG     F D+  + 
Sbjct: 360 LVYAAILGSSSADRYNLKPSP---PC--FPKLLSHNGSNAIGSLRLGKYTKWFNDV--SS 419

Query: 312 SMAIPTFEHHVNLLRKSGATIVDNLQISNIDTILDPY----GSGELIATIAEFKLAINDY 371
           S      E  + LL  +    V  + +  ++ +   +    GS  L +     +   N  
Sbjct: 420 SDISDKCEDILKLLSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCEAGKNSK 479

Query: 372 LKNLIHSPVR-----SLADIIAFNNNHPELEKMN-EYGQDAFLLSDQTDGIGKAEEEAIS 431
           L     +        S +D IA       L + +    +D  ++   T G+      A  
Sbjct: 480 LSYDTRTSFAIFRSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGM-----TAPV 539

Query: 432 MMANLSRDGFELMMKTYNLDAMVTLGTSAESVLAIGGYPAISVPAGYEGNGDPFGILFGG 491
           +  +  ++G   +  T +L   V     A ++L   G+PAISVP GY+  G P G+   G
Sbjct: 540 IPPDALKNGETNIQVTTDLMRFVL----AANLL---GFPAISVPVGYDKEGLPIGLQIMG 589

Query: 492 LRGTETKLIEIAYAFEQ 495
               E  ++ +A A E+
Sbjct: 600 RPWAEATVLGLAAAVEE 589

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1P8B7605.6e-15962.45Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY47.8e-7638.52Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C82.3e-6434.08Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
B0JSX32.3e-4331.84Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain ... [more]
Q8DK652.5e-4231.79Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermosynechococcus elongatus (... [more]
Match NameE-valueIdentityDescription
XP_022136311.10.0100.00putative amidase C869.01 [Momordica charantia][more]
XP_023529660.13.32e-30687.10probable amidase At4g34880 [Cucurbita pepo subsp. pepo][more]
XP_022928333.13.63e-30686.90putative amidase C869.01 [Cucurbita moschata][more]
KAG7015266.11.47e-30586.49hypothetical protein SDJN02_22900 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6589001.15.99e-30586.29putative amidase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1C3630.0100.00putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111008028 PE=4 SV=... [more]
A0A6J1ENQ31.76e-30686.90putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111435192 PE=4 SV=1[more]
A0A6J1JLA21.23e-30285.89putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111486234 PE=4 SV=1[more]
A0A1S3BIN02.43e-28783.03putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103490302 PE=4 SV=1[more]
A0A0A0K1883.51e-28682.70Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009770 PE=... [more]
Match NameE-valueIdentityDescription
AT4G34880.13.1e-13655.39Amidase family protein [more]
AT3G25660.15.1e-3027.62Amidase family protein [more]
AT5G07360.11.1e-2732.84Amidase family protein [more]
AT5G07360.24.9e-2532.46Amidase family protein [more]
AT5G64440.11.1e-1627.00fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 44..485
e-value: 9.9E-72
score: 242.4
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 11..501
e-value: 6.2E-143
score: 479.4
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 22..496
NoneNo IPR availablePANTHERPTHR42678:SF25C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 9..498
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 9..498

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC04g0408.1MC04g0408.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane