Tan0012852 (gene) Snake gourd v1

Overview
NameTan0012852
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionheavy metal-associated isoprenylated plant protein 9-like
LocationLG01: 560959 .. 562774 (+)
RNA-Seq ExpressionTan0012852
SyntenyTan0012852
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGTGAAGTGATGAAAGAAGGCAAAAAGAAATTAGAAAGGGAAGGGAGGTGAGTAATAAATACTTAATAGGGATATTGAGTATTTAGTATTGACCACTTTCAGTTTGTGTGTGTGTGTGTGTGTGTGTATGTGAAATATAATCCAACGCCCCCTTCTTCTCTGATATATATCCTCCTTTTCTACATTTCTTAATTAACTTGACTTGTTCCCCAATCCTTCTTCTTCTTATAAATTCCTTTGCGCTCAATTACACATCTAACTCTGCCCTGCTTTCTTCCAATTACTTCTCTGCTTTCCCTTTCCCTTTCCCCTACCAACACACAAACACAAACACAAACACAGCACCCGATCGAGATGGGCGAAGAAGCTGTTAAGATGGTGAGTTATCTTTTTAAATTTATTGTAGTTATATAGTGAGCAGCCGCCATATGCATTGACTTCTAACCCCCCACGCTGGTGCATCAGGAAGAACCAAAGGTAGAAGAGACGACGAAAGTGGAAGAATCCAAACCTTCTCCTCCTTTCGTCTTATTTGTGGATCTTCATTGCACCGGATGTGCAAAGAAAATTGAAAAGTCCCTCATGAGAATTAGAGGTGCGCTAGGATGTGTTCTGTTTCCTTTCCTTTCCTTTCCATTCATTGCTATATTTATATTAATAATGAATGGTAAATTGGTAATGCACCAGGAGTGGAAGGGGTGAGCATTGACATGGCCAAAAATGAAGTAAGCATCAAAGGAATAATAGTAGACCCAGACCCAGAGGCCATTTGCGCTAAAATCACCAAGAAGACCAAGAGAGCAGCCAAAGTGTTGTCCCCATTGCCACCGCCTCAGGGCGAACCCATCCCCCAACTTCTCAACTCCCAGGTATCAAGCCCATTCATAATTAATTAATGCCATTTTGCATGCTTTCATTTCATTTGTTTTATAATTATATTGATTTAACTTTCACGGAAGAAGGTGGGTGGATTGACGCTGGTGGAATTGAACGTTAACATGCACTGCCAGGCCTGTGCTCTGCAACTCAAGAAAAAGATACTCAAAATGAGAGGTAAAATTAATTATTACTCATGACCCTTCAATTGCAAATTAATTATTAATTAATTCATTCAAATTTTTCCCAGGAGTGCAAACAGCATCGACAGAATTGAGCACAGGAAAAGTGATGGTAACAGGAACCATGGACGGGGATAAGCTAGTGGAGTACGTGTATAGACGCACCAAAAAGCAAGCAAGAATAGTGCCACAGCCGGAACCAGCTGAAATTAACAAGCCACCAGAAAAAGACGAAGAAGAACCACCAAAATTAGCCGAAGAAAAGAAAGAAGATCATGATCAGGAACCACCACCACCACCAGAAGTGACAAAGACAAAGACAGAGATAGAAGAAGACGGAAATATTAACAAGAAGGAAGACGATCCAGATCATCAACAACCAAAACCAGCAACCACCACTACTACAGTGGAACCGACGAAGCCAGGGGAGGGCGGAGATCAGATTATAATAATGGATGATCATGAAAACATGAAGAGGATGATGTATCACTATCACTATCACCCACTTTACGTTATCGAACCAATTCCACCGCCTCAGCTATTCAGCGATGAGAATCCCAATGCTTGTTGCATCCTATAAAATAAAACTACTTTCCAATTCCCACTCAAAATCCCCCCCTTTTTTTTAACTGTATACTAATCAAATCACCCTTCCCTCATATTAATGCCATCTCTACGCATCTAATTCTCCGACTCAATCTATCTAAATGATTAAAATAATTAAAAATAGTTTACCACAAGATAAATTTGTAAATAC

mRNA sequence

GTGTGAAGTGATGAAAGAAGGCAAAAAGAAATTAGAAAGGGAAGGGAGGTGAGTAATAAATACTTAATAGGGATATTGAGTATTTAGTATTGACCACTTTCAGTTTGTGTGTGTGTGTGTGTGTGTGTATGTGAAATATAATCCAACGCCCCCTTCTTCTCTGATATATATCCTCCTTTTCTACATTTCTTAATTAACTTGACTTGTTCCCCAATCCTTCTTCTTCTTATAAATTCCTTTGCGCTCAATTACACATCTAACTCTGCCCTGCTTTCTTCCAATTACTTCTCTGCTTTCCCTTTCCCTTTCCCCTACCAACACACAAACACAAACACAAACACAGCACCCGATCGAGATGGGCGAAGAAGCTGTTAAGATGGAAGAACCAAAGGTAGAAGAGACGACGAAAGTGGAAGAATCCAAACCTTCTCCTCCTTTCGTCTTATTTGTGGATCTTCATTGCACCGGATGTGCAAAGAAAATTGAAAAGTCCCTCATGAGAATTAGAGGAGTGGAAGGGGTGAGCATTGACATGGCCAAAAATGAAGTAAGCATCAAAGGAATAATAGTAGACCCAGACCCAGAGGCCATTTGCGCTAAAATCACCAAGAAGACCAAGAGAGCAGCCAAAGTGTTGTCCCCATTGCCACCGCCTCAGGGCGAACCCATCCCCCAACTTCTCAACTCCCAGGTGGGTGGATTGACGCTGGTGGAATTGAACGTTAACATGCACTGCCAGGCCTGTGCTCTGCAACTCAAGAAAAAGATACTCAAAATGAGAGGAGTGCAAACAGCATCGACAGAATTGAGCACAGGAAAAGTGATGGTAACAGGAACCATGGACGGGGATAAGCTAGTGGAGTACGTGTATAGACGCACCAAAAAGCAAGCAAGAATAGTGCCACAGCCGGAACCAGCTGAAATTAACAAGCCACCAGAAAAAGACGAAGAAGAACCACCAAAATTAGCCGAAGAAAAGAAAGAAGATCATGATCAGGAACCACCACCACCACCAGAAGTGACAAAGACAAAGACAGAGATAGAAGAAGACGGAAATATTAACAAGAAGGAAGACGATCCAGATCATCAACAACCAAAACCAGCAACCACCACTACTACAGTGGAACCGACGAAGCCAGGGGAGGGCGGAGATCAGATTATAATAATGGATGATCATGAAAACATGAAGAGGATGATGTATCACTATCACTATCACCCACTTTACGTTATCGAACCAATTCCACCGCCTCAGCTATTCAGCGATGAGAATCCCAATGCTTGTTGCATCCTATAAAATAAAACTACTTTCCAATTCCCACTCAAAATCCCCCCCTTTTTTTTAACTGTATACTAATCAAATCACCCTTCCCTCATATTAATGCCATCTCTACGCATCTAATTCTCCGACTCAATCTATCTAAATGATTAAAATAATTAAAAATAGTTTACCACAAGATAAATTTGTAAATAC

Coding sequence (CDS)

ATGGGCGAAGAAGCTGTTAAGATGGAAGAACCAAAGGTAGAAGAGACGACGAAAGTGGAAGAATCCAAACCTTCTCCTCCTTTCGTCTTATTTGTGGATCTTCATTGCACCGGATGTGCAAAGAAAATTGAAAAGTCCCTCATGAGAATTAGAGGAGTGGAAGGGGTGAGCATTGACATGGCCAAAAATGAAGTAAGCATCAAAGGAATAATAGTAGACCCAGACCCAGAGGCCATTTGCGCTAAAATCACCAAGAAGACCAAGAGAGCAGCCAAAGTGTTGTCCCCATTGCCACCGCCTCAGGGCGAACCCATCCCCCAACTTCTCAACTCCCAGGTGGGTGGATTGACGCTGGTGGAATTGAACGTTAACATGCACTGCCAGGCCTGTGCTCTGCAACTCAAGAAAAAGATACTCAAAATGAGAGGAGTGCAAACAGCATCGACAGAATTGAGCACAGGAAAAGTGATGGTAACAGGAACCATGGACGGGGATAAGCTAGTGGAGTACGTGTATAGACGCACCAAAAAGCAAGCAAGAATAGTGCCACAGCCGGAACCAGCTGAAATTAACAAGCCACCAGAAAAAGACGAAGAAGAACCACCAAAATTAGCCGAAGAAAAGAAAGAAGATCATGATCAGGAACCACCACCACCACCAGAAGTGACAAAGACAAAGACAGAGATAGAAGAAGACGGAAATATTAACAAGAAGGAAGACGATCCAGATCATCAACAACCAAAACCAGCAACCACCACTACTACAGTGGAACCGACGAAGCCAGGGGAGGGCGGAGATCAGATTATAATAATGGATGATCATGAAAACATGAAGAGGATGATGTATCACTATCACTATCACCCACTTTACGTTATCGAACCAATTCCACCGCCTCAGCTATTCAGCGATGAGAATCCCAATGCTTGTTGCATCCTATAA

Protein sequence

MGEEAVKMEEPKVEETTKVEESKPSPPFVLFVDLHCTGCAKKIEKSLMRIRGVEGVSIDMAKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPIPQLLNSQVGGLTLVELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRTKKQARIVPQPEPAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIEEDGNINKKEDDPDHQQPKPATTTTTVEPTKPGEGGDQIIIMDDHENMKRMMYHYHYHPLYVIEPIPPPQLFSDENPNACCIL
Homology
BLAST of Tan0012852 vs. ExPASy Swiss-Prot
Match: Q9FLU5 (Heavy metal-associated isoprenylated plant protein 9 OS=Arabidopsis thaliana OX=3702 GN=HIPP09 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.3e-78
Identity = 176/310 (56.77%), Postives = 215/310 (69.35%), Query Frame = 0

Query: 3   EEAVKMEEPKVEETTKVEESKPSPPFVLFVDLHCTGCAKKIEKSLMRIRGVEGVSIDMAK 62
           E+ V  EE K +E  + +   P PPF+L+VDLHC GCAKKIE+S+++IRGVE V +DM +
Sbjct: 33  EKKVAAEEEKPKEEEEPQPPPPPPPFILYVDLHCVGCAKKIERSILKIRGVEEVVMDMNE 92

Query: 63  NEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPIPQLLNSQV-GGLTLVEL 122
           N+V+IKG++   DP+A+C KI KKTKR AKVLSPLP  +GEP+P ++ SQV GGLT VEL
Sbjct: 93  NQVTIKGVL---DPQAVCNKIKKKTKRMAKVLSPLPAAEGEPLPPIITSQVSGGLTTVEL 152

Query: 123 NVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRTKKQARI 182
           +VNMHCQACA QLKKKILKMRGVQT  TE +TGKV+VTGTMD +KLV+YVYRRTKKQARI
Sbjct: 153 SVNMHCQACADQLKKKILKMRGVQTTVTEHTTGKVIVTGTMDAEKLVDYVYRRTKKQARI 212

Query: 183 VPQPEPAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIEEDGNINKKEDD 242
           VPQP+P         + E P    EEKKE+  +    PPE   T  E EE+    KKE +
Sbjct: 213 VPQPDP---------EPEAPAAAQEEKKEESGEGNEKPPE---TGEEKEEE---KKKEGE 272

Query: 243 PDHQQPKPATTTTTVEPTKPGEGGDQIIIMDDHENMKRMMYHYHYHPLYVIEPIPPPQLF 302
            + ++        T E  +  E    +  M   E MKRMM  Y+Y P YVIE IPPPQLF
Sbjct: 273 ENGEEGGGEEAAATEEERRDNE----MTAMAQEEGMKRMM--YYYQPSYVIERIPPPQLF 318

Query: 303 SDENPNACCI 312
           SDENPNACCI
Sbjct: 333 SDENPNACCI 318

BLAST of Tan0012852 vs. ExPASy Swiss-Prot
Match: Q9C5D3 (Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=3702 GN=HIPP07 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 3.0e-32
Identity = 111/322 (34.47%), Postives = 168/322 (52.17%), Query Frame = 0

Query: 14  EETTKVEESKPSPP-----FVLFVDLHCTGCAKKIEKSLMRIRGVEGVSIDMAKNEVSIK 73
           E++       P+PP      VL V +HC GCA+K+ + L    GVE V  D    +V +K
Sbjct: 56  EDSAPAAPEAPAPPPPPQEVVLKVYMHCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVK 115

Query: 74  GIIVDPDPEAICAKITKKTKRAAKVLSPLPPP---------QGEPIPQLLNSQVGGLTLV 133
           G     DP  + A++ +KT R  ++LSP+PPP         + +PI +    +   +  V
Sbjct: 116 G--EKADPLKVLARVQRKTHRQVQLLSPIPPPPPPPEKKAEEDKPIVEEKKVEPPVVVTV 175

Query: 134 ELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRTKKQA 193
            L V+MHC+ACA ++KK+I++M+GV++A ++L + +V V G  +  KLVEYVY+RT K A
Sbjct: 176 VLKVHMHCEACATEIKKRIMRMKGVESAESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHA 235

Query: 194 RIVPQPEPAEINKPPEKDEEEPPKLAE-EKKEDHDQEPPPPPEVTK-TKTEIEEDGNINK 253
            I+      +I+ PP    EE    AE EKKE+   E     E  K  K + +E+    K
Sbjct: 236 AIM------KIDPPPPPPPEEAAAAAEGEKKEEEKGEGESKGEEGKDDKAKTDEE----K 295

Query: 254 KEDDPDHQQPKPATTTTTVEPTKPGEGGDQIIIMDDHENMKRMMYHYHYHPLYVIEP--- 313
           KE D    + + A             GG++   + +   ++   Y+Y+Y P  V  P   
Sbjct: 296 KEGDGGKGEGEAA----------DNGGGEEEGKVVEVRKIENPYYYYYYQPPRVAIPPME 355

BLAST of Tan0012852 vs. ExPASy Swiss-Prot
Match: Q9SHQ8 (Heavy metal-associated isoprenylated plant protein 4 OS=Arabidopsis thaliana OX=3702 GN=HIPP04 PE=3 SV=2)

HSP 1 Score: 101.3 bits (251), Expect = 2.0e-20
Identity = 88/309 (28.48%), Postives = 142/309 (45.95%), Query Frame = 0

Query: 14  EETTKVEESKPSPPFVLFVDLHCTGCAKKIEKSLMRIRGVEGVSIDMAKNEVSIKGIIVD 73
           EE  K E+       V  V LHC  CA  I+K L+R +GV+ V  D+ KNE+ +KG I  
Sbjct: 3   EEEEKKEKGDEIITAVYKVHLHCRKCACDIKKPLLRFQGVQNVDFDLEKNEIKVKGKI-- 62

Query: 74  PDPEAICAKITKKTKRAAKVLSPLPPPQGEPIPQLLNSQVGGLTLVE----------LNV 133
            +   I  +I K +K+  +++SP P    +       + V      E          L V
Sbjct: 63  -EVVKIHKQIEKWSKKKVELISPKPSEVKKTTTTTTTTSVVEKKTTEIKKDVIRTTVLKV 122

Query: 134 NMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRTKKQARIVP 193
           ++HC  C   L+ K+LK + +    T+     + V GT++  KL+ Y+ ++  K A I+ 
Sbjct: 123 HIHCAQCDKDLQHKLLKHKAIHIVKTDTKAQTLTVQGTIESAKLLAYIKKKVHKHAEII- 182

Query: 194 QPEPAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIEEDGNINKKEDDPD 253
                       K EEE  K  E+KK+  +++     +  K + E +++    KKE +  
Sbjct: 183 ----------SSKTEEEKKKEEEDKKKKEEEDKKKKEDEKKKEEEKKKEEENKKKEGEKK 242

Query: 254 HQQPKPATTTTTVEPTKPGEGGDQIIIMDDHENMKRMMYHYHYHPLYVIEPIPPPQLFSD 313
            ++ K   TT T+  T+  E  + + +    +    + Y  HY        +  PQLFSD
Sbjct: 243 KEEVKVEVTTKTI--TQVVEYKEIVKVEGQKDKDGNIPYFVHY--------VYAPQLFSD 287

BLAST of Tan0012852 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 6.8e-16
Identity = 90/335 (26.87%), Postives = 141/335 (42.09%), Query Frame = 0

Query: 1   MGEEAVKMEEPKVEETTKVEESKPSPPFVLFVDLHCTGCAKKIEKSLMRIRGVEGVSIDM 60
           MGE+  + +  K     K +   PS   VL VD+HC GCA +I K +   +GVE V  + 
Sbjct: 1   MGEKKNEGDNKKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSES 60

Query: 61  AKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPIPQLLNSQ-------- 120
           A  ++++ G +   DP  +  K+ +KTK+   ++SP P  + E   +  N +        
Sbjct: 61  ATGKLTVTGAL---DPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEK 120

Query: 121 -----------VGGLTLVELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGT 180
                         +T   L +N HCQ C  +++K + K +GV   + +     + V GT
Sbjct: 121 KKPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGT 180

Query: 181 MDGDKLVEYVYRRTKKQARIVPQPEPAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPE 240
           MD  KLVE +  + K+   IVP  +        EKD+E   +  E+KK            
Sbjct: 181 MDVKKLVEILSEKLKRAVEIVPPKK--------EKDKENGNENGEKKKGGGG-------- 240

Query: 241 VTKTKTEIEEDGNINKKEDDPDHQQPKPATTTTTVEPTKPGEGGDQIIIMDDHENMKRMM 300
                     DG   +K                    T    GG+ + +M+      +  
Sbjct: 241 ----------DGGGKEK--------------------TGNKGGGEGVNMME--YMAAQPA 283

Query: 301 YHYHYHP----LYVIEPIPPPQLFSDENPNACCIL 313
           Y Y Y+P     Y I+    PQ+FSDENPNAC ++
Sbjct: 301 YGYGYYPGGPYGYPIQ-AHAPQIFSDENPNACVVM 283

BLAST of Tan0012852 vs. ExPASy Swiss-Prot
Match: Q9M8T7 (Heavy metal-associated isoprenylated plant protein 8 OS=Arabidopsis thaliana OX=3702 GN=HIPP08 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 7.5e-15
Identity = 59/196 (30.10%), Postives = 99/196 (50.51%), Query Frame = 0

Query: 7   KMEEPKVEETTKVEESKPSPPFVLFVDLHCTGCAKKIEKSLMRIRGVEGVSIDMAKNEVS 66
           K  +  V+++ K  + K     VL V +HC GCA ++   L    GVE +  ++  N+V 
Sbjct: 20  KNGDSSVDKSDKKNQCK---EIVLKVYMHCEGCASQVSHCLRGYDGVEHIKTEIGDNKVV 79

Query: 67  IKGIIVDPDPEAICAKITKKTKRAAKVLSPL-PPPQGEPIPQLLNSQVGGLTLVELNVNM 126
           + G     DP  I  ++ KK  R A+++SP   P Q +  PQ        +    L +NM
Sbjct: 80  VSGKF--DDPLKILRRVQKKFSRNAEMISPKHNPKQDQKEPQQKKESAPEIKTAILRMNM 139

Query: 127 HCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRTKKQARIVPQ- 186
           HC+ C  ++K+ I K++G+Q+   + S   V+V G MD  KLVE + ++  K A ++ Q 
Sbjct: 140 HCEGCVHEIKRGIEKIKGIQSVEPDRSKSTVVVRGVMDPPKLVEKIKKKLGKHAELLSQI 199

Query: 187 PEPAEINKPPEKDEEE 201
            E  + N     +++E
Sbjct: 200 TEKGKDNNKKNNNKKE 210

BLAST of Tan0012852 vs. NCBI nr
Match: XP_023541673.1 (heavy metal-associated isoprenylated plant protein 9-like [Cucurbita pepo subsp. pepo] >XP_023541674.1 heavy metal-associated isoprenylated plant protein 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 382.5 bits (981), Expect = 3.5e-102
Identity = 233/329 (70.82%), Postives = 261/329 (79.33%), Query Frame = 0

Query: 1   MGEEAVKMEEPKVEET---TKVEESKPSPPFV-LFVDLHCTGCAKKIEKSLMRIRGVEGV 60
           MG EAVKMEE KVEET    KVE+ KP PP V LFVDLHCTGCAKKIEKSLMRI GVEGV
Sbjct: 1   MGGEAVKMEEAKVEETKEELKVEKPKPCPPSVLLFVDLHCTGCAKKIEKSLMRITGVEGV 60

Query: 61  SIDMAKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPI-PQLLNSQVGG 120
           SI++AKNE++IKGI+   DP A+CAKITKKTKR AKVLSPLPP +GEP  PQL+NSQVGG
Sbjct: 61  SINVAKNELTIKGIV---DPHAVCAKITKKTKRVAKVLSPLPPAEGEPAGPQLVNSQVGG 120

Query: 121 LTLVELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRT 180
           LT+VEL VNMHC+ACALQLK+KILKMRGVQTA TELSTGKV+VTG+MDG KLVEYVYRRT
Sbjct: 121 LTVVELKVNMHCEACALQLKRKILKMRGVQTAWTELSTGKVVVTGSMDGSKLVEYVYRRT 180

Query: 181 KKQARIVPQPE-PAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIEE--D 240
           KKQARIVPQPE P + NKPP   +EEPPK AEEKKED       PP  TKT+  + +  D
Sbjct: 181 KKQARIVPQPEPPQQENKPP---QEEPPK-AEEKKEDEAAAAAAPPPETKTEDALPQGTD 240

Query: 241 GNINKKEDDPDHQQP----KPA--TTTTTVEPTKPGEGGDQIIIMD--DHENMKRMMYH- 300
            N NK+E+ P   +P    +PA  T  ++VE TKP EGGD+ I+MD  D E MKRM+YH 
Sbjct: 241 TNNNKEEEQPKPTEPAEPGEPAEETKASSVE-TKPAEGGDEKIVMDEVDPEIMKRMIYHN 300

Query: 301 YHYHPLYVIEPI-PPPQLFSDENPNACCI 312
           Y Y PLYVIEPI PPPQLFSDENPNACCI
Sbjct: 301 YQYQPLYVIEPIPPPPQLFSDENPNACCI 321

BLAST of Tan0012852 vs. NCBI nr
Match: XP_022968630.1 (heavy metal-associated isoprenylated plant protein 9-like [Cucurbita maxima])

HSP 1 Score: 378.6 bits (971), Expect = 5.1e-101
Identity = 231/327 (70.64%), Postives = 256/327 (78.29%), Query Frame = 0

Query: 1   MGEEAVKMEEPKVEET---TKVEESKPSPPFV-LFVDLHCTGCAKKIEKSLMRIRGVEGV 60
           MGEEAVKMEE KVEET    KVE+ KP PP V LFVDLHCTGCAKKIEKSLMRI GVEGV
Sbjct: 1   MGEEAVKMEEAKVEETKEELKVEKPKPCPPSVLLFVDLHCTGCAKKIEKSLMRITGVEGV 60

Query: 61  SIDMAKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPI-PQLLNSQVGG 120
           SI++AKNE++IKGI+   DP A+CAKITKKTKR AKVLSPLPP +GEP  PQL+NSQVGG
Sbjct: 61  SINVAKNELTIKGIV---DPHAVCAKITKKTKRVAKVLSPLPPAEGEPTGPQLVNSQVGG 120

Query: 121 LTLVELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRT 180
           LT+VEL VNMHC+ACALQLK+KILKMRGVQTA TELSTGKV+VTG+MDG KLVEYVYRRT
Sbjct: 121 LTVVELKVNMHCEACALQLKRKILKMRGVQTAWTELSTGKVVVTGSMDGSKLVEYVYRRT 180

Query: 181 KKQARIVPQPE-PAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIEEDGN 240
           KKQARIVPQPE P + NKPP   +EEPPK AEEKKED      PP   T+       D N
Sbjct: 181 KKQARIVPQPEPPQQENKPP---QEEPPK-AEEKKEDEAAAAAPPETKTEDALPQGTDTN 240

Query: 241 INKKEDDPDHQQP----KPA--TTTTTVEPTKPGEGGDQIIIMD--DHENMKRMMYH-YH 300
            NK+E+ P   +P    +PA  T  ++VE  K  EGGD+ IIMD  D ENMKRM+YH Y 
Sbjct: 241 NNKEEEQPKPTEPVEPGEPAEETKASSVE-AKQAEGGDETIIMDEVDPENMKRMIYHNYQ 300

Query: 301 YHPLYVIEPI-PPPQLFSDENPNACCI 312
           Y  LYVIEPI PPPQLFSDENPNACCI
Sbjct: 301 YQSLYVIEPIPPPPQLFSDENPNACCI 319

BLAST of Tan0012852 vs. NCBI nr
Match: XP_022944904.1 (heavy metal-associated isoprenylated plant protein 9-like [Cucurbita moschata] >XP_022944905.1 heavy metal-associated isoprenylated plant protein 9-like [Cucurbita moschata] >XP_022944906.1 heavy metal-associated isoprenylated plant protein 9-like [Cucurbita moschata] >KAG6574269.1 Heavy metal-associated isoprenylated plant protein 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 375.6 bits (963), Expect = 4.3e-100
Identity = 230/331 (69.49%), Postives = 256/331 (77.34%), Query Frame = 0

Query: 1   MGEEAVKMEEPKVEET---TKVEESKPSPPFV-LFVDLHCTGCAKKIEKSLMRIRGVEGV 60
           MGEEAVKMEE KVEET    KVE+ KP PP V LFVDLHCTGCAKKIEKSLMRI GVEGV
Sbjct: 1   MGEEAVKMEEAKVEETKEELKVEKPKPCPPSVLLFVDLHCTGCAKKIEKSLMRITGVEGV 60

Query: 61  SIDMAKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPI-PQLLNSQVGG 120
           S ++AKNE++IKGI+   DP A+CAKITKKTKR AKVLSPLPP +GEP  PQL+NSQVGG
Sbjct: 61  STNVAKNELTIKGIV---DPHAVCAKITKKTKRVAKVLSPLPPAEGEPTGPQLVNSQVGG 120

Query: 121 LTLVELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRT 180
           LT+VEL VNMHC+ACALQLK+KILKMRGVQTA TELSTGKV+VTG+MDG KLVEYVYRRT
Sbjct: 121 LTVVELKVNMHCEACALQLKRKILKMRGVQTAWTELSTGKVVVTGSMDGSKLVEYVYRRT 180

Query: 181 KKQARIVPQPE-PAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIE---- 240
           KKQARIVPQPE P + NKPP   +EEPPK AEEKKED           TKTKTE      
Sbjct: 181 KKQARIVPQPEPPQQENKPP---QEEPPK-AEEKKEDE----------TKTKTEDALPQG 240

Query: 241 EDGNINKKEDDP------DHQQPKPATTTTTVEPTKPGEGGDQIIIMD--DHENMKRMMY 300
            D N NK+E+ P      +  +P   T  ++VE  KP EGG++ I+MD  D E MKRM+Y
Sbjct: 241 TDTNNNKEEEQPKPTEPAEPGEPGEETKASSVE-AKPAEGGEEKIVMDEVDPEIMKRMIY 300

Query: 301 H-YHYHPLYVIEPI-PPPQLFSDENPNACCI 312
           H Y Y PLYVIEPI PPPQLFSDENPNACCI
Sbjct: 301 HNYQYQPLYVIEPIPPPPQLFSDENPNACCI 313

BLAST of Tan0012852 vs. NCBI nr
Match: KAG7013337.1 (Heavy metal-associated isoprenylated plant protein 9 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 372.1 bits (954), Expect = 4.7e-99
Identity = 229/334 (68.56%), Postives = 255/334 (76.35%), Query Frame = 0

Query: 1   MGEEAVKMEEPKVEET---TKVEESKPSPPFV-LFVDLHCTGCAKKIEKSLMRIRGVEGV 60
           MGEEAVKMEE KVEET    KVE+ KP PP V LFVDLHCTGCAKKIEKSLMRI GVEGV
Sbjct: 1   MGEEAVKMEEAKVEETKEELKVEKPKPCPPSVLLFVDLHCTGCAKKIEKSLMRITGVEGV 60

Query: 61  SIDMAKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPI-PQLLNSQVGG 120
           S ++AKNE++IKGI+   DP A+CAKITKKTKR AKVLSPLPP +G P  PQL+NSQVGG
Sbjct: 61  STNVAKNELTIKGIV---DPHAVCAKITKKTKRVAKVLSPLPPAEGAPTGPQLVNSQVGG 120

Query: 121 LTLVELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRT 180
           LT+VEL VNMHC+ACALQLK+KILKMRGVQTA TELSTGKV+VTG+MDG KLVEYVYRRT
Sbjct: 121 LTVVELKVNMHCEACALQLKRKILKMRGVQTAWTELSTGKVVVTGSMDGSKLVEYVYRRT 180

Query: 181 KKQARIVPQPE-PAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIE---- 240
           KKQARIVPQPE P + NKPP   +EEPPK AEEKKED           TKTKTE      
Sbjct: 181 KKQARIVPQPEPPQQENKPP---QEEPPK-AEEKKEDE----------TKTKTEDALPQG 240

Query: 241 EDGNINKKEDDP---------DHQQPKPATTTTTVEPTKPGEGGDQIIIMD--DHENMKR 300
            D N NK+E+ P         +  +P   T  ++VE  KP EGG++ I+MD  D E MKR
Sbjct: 241 TDTNNNKEEEQPKPTEPAEPGEPGEPGEETKASSVE-AKPAEGGEEKIVMDEVDPEIMKR 300

Query: 301 MMYH-YHYHPLYVIEPI-PPPQLFSDENPNACCI 312
           M+YH Y Y PLYVIEPI PPPQLFSDENPNACCI
Sbjct: 301 MIYHNYQYQPLYVIEPIPPPPQLFSDENPNACCI 316

BLAST of Tan0012852 vs. NCBI nr
Match: XP_023551857.1 (heavy metal-associated isoprenylated plant protein 9 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 347.8 bits (891), Expect = 9.6e-92
Identity = 217/337 (64.39%), Postives = 245/337 (72.70%), Query Frame = 0

Query: 1   MGEEAVKM-----EEPKVEETTKVEESK--PSPPFVLFVDLHCTGCAKKIEKSLMRIRGV 60
           MGEEAV+M     EE KVEE  K+EE+K  P PP VL VDLHC+GCAKKIEK +MRIRGV
Sbjct: 1   MGEEAVQMQQQQQEEAKVEE-MKLEEAKPCPCPPIVLLVDLHCSGCAKKIEKCIMRIRGV 60

Query: 61  EGVSIDMAKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPP-PQGEPIPQLL-NS 120
           EGVSIDMAKNEV+IKGI+   DP+A+CAKIT KTKR AKVLSP PP P+GEP P L+ NS
Sbjct: 61  EGVSIDMAKNEVTIKGIV---DPDAVCAKITTKTKRVAKVLSPSPPLPEGEPSPHLIVNS 120

Query: 121 QVGGLTLVELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYV 180
           Q   L +VELNVNMHC ACA QLKKKILKMRGVQTASTELSTGKV+VTGTMDGDKLV+YV
Sbjct: 121 Q---LKVVELNVNMHCDACAHQLKKKILKMRGVQTASTELSTGKVVVTGTMDGDKLVDYV 180

Query: 181 YRRTKKQARIVPQPEPAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKT------ 240
           YRRTKKQARIVPQP+P     PP   EEEP K  E K+E     PPPPPE  KT      
Sbjct: 181 YRRTKKQARIVPQPQPTA--TPP---EEEPLKPEESKEEQAAPPPPPPPEEIKTEDAAAP 240

Query: 241 KTEIEEDGNINKKEDDPDHQQ----PKPATTTTT-----VEPTKPGEGGDQIIIMDDHEN 300
           + +  +  N NK+E  P   +    P    T  T     VE  K  E GD+++  DDHE+
Sbjct: 241 QAQGTDTNNNNKEEVQPKAAEEGGGPVVEATAETKPNVEVEEAKAAEAGDEMMGEDDHES 300

Query: 301 MKRMMY-HYHYHPLYVIEPIPPPQLFSDENPNACCIL 313
           MKRMMY +Y Y PLYV+E IPPPQLFSDENPNACC+L
Sbjct: 301 MKRMMYQYYQYQPLYVMERIPPPQLFSDENPNACCVL 325

BLAST of Tan0012852 vs. ExPASy TrEMBL
Match: A0A6J1HXR0 (heavy metal-associated isoprenylated plant protein 9-like OS=Cucurbita maxima OX=3661 GN=LOC111467792 PE=4 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 2.5e-101
Identity = 231/327 (70.64%), Postives = 256/327 (78.29%), Query Frame = 0

Query: 1   MGEEAVKMEEPKVEET---TKVEESKPSPPFV-LFVDLHCTGCAKKIEKSLMRIRGVEGV 60
           MGEEAVKMEE KVEET    KVE+ KP PP V LFVDLHCTGCAKKIEKSLMRI GVEGV
Sbjct: 1   MGEEAVKMEEAKVEETKEELKVEKPKPCPPSVLLFVDLHCTGCAKKIEKSLMRITGVEGV 60

Query: 61  SIDMAKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPI-PQLLNSQVGG 120
           SI++AKNE++IKGI+   DP A+CAKITKKTKR AKVLSPLPP +GEP  PQL+NSQVGG
Sbjct: 61  SINVAKNELTIKGIV---DPHAVCAKITKKTKRVAKVLSPLPPAEGEPTGPQLVNSQVGG 120

Query: 121 LTLVELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRT 180
           LT+VEL VNMHC+ACALQLK+KILKMRGVQTA TELSTGKV+VTG+MDG KLVEYVYRRT
Sbjct: 121 LTVVELKVNMHCEACALQLKRKILKMRGVQTAWTELSTGKVVVTGSMDGSKLVEYVYRRT 180

Query: 181 KKQARIVPQPE-PAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIEEDGN 240
           KKQARIVPQPE P + NKPP   +EEPPK AEEKKED      PP   T+       D N
Sbjct: 181 KKQARIVPQPEPPQQENKPP---QEEPPK-AEEKKEDEAAAAAPPETKTEDALPQGTDTN 240

Query: 241 INKKEDDPDHQQP----KPA--TTTTTVEPTKPGEGGDQIIIMD--DHENMKRMMYH-YH 300
            NK+E+ P   +P    +PA  T  ++VE  K  EGGD+ IIMD  D ENMKRM+YH Y 
Sbjct: 241 NNKEEEQPKPTEPVEPGEPAEETKASSVE-AKQAEGGDETIIMDEVDPENMKRMIYHNYQ 300

Query: 301 YHPLYVIEPI-PPPQLFSDENPNACCI 312
           Y  LYVIEPI PPPQLFSDENPNACCI
Sbjct: 301 YQSLYVIEPIPPPPQLFSDENPNACCI 319

BLAST of Tan0012852 vs. ExPASy TrEMBL
Match: A0A6J1FZC7 (heavy metal-associated isoprenylated plant protein 9-like OS=Cucurbita moschata OX=3662 GN=LOC111449296 PE=4 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 2.1e-100
Identity = 230/331 (69.49%), Postives = 256/331 (77.34%), Query Frame = 0

Query: 1   MGEEAVKMEEPKVEET---TKVEESKPSPPFV-LFVDLHCTGCAKKIEKSLMRIRGVEGV 60
           MGEEAVKMEE KVEET    KVE+ KP PP V LFVDLHCTGCAKKIEKSLMRI GVEGV
Sbjct: 1   MGEEAVKMEEAKVEETKEELKVEKPKPCPPSVLLFVDLHCTGCAKKIEKSLMRITGVEGV 60

Query: 61  SIDMAKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPI-PQLLNSQVGG 120
           S ++AKNE++IKGI+   DP A+CAKITKKTKR AKVLSPLPP +GEP  PQL+NSQVGG
Sbjct: 61  STNVAKNELTIKGIV---DPHAVCAKITKKTKRVAKVLSPLPPAEGEPTGPQLVNSQVGG 120

Query: 121 LTLVELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRT 180
           LT+VEL VNMHC+ACALQLK+KILKMRGVQTA TELSTGKV+VTG+MDG KLVEYVYRRT
Sbjct: 121 LTVVELKVNMHCEACALQLKRKILKMRGVQTAWTELSTGKVVVTGSMDGSKLVEYVYRRT 180

Query: 181 KKQARIVPQPE-PAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIE---- 240
           KKQARIVPQPE P + NKPP   +EEPPK AEEKKED           TKTKTE      
Sbjct: 181 KKQARIVPQPEPPQQENKPP---QEEPPK-AEEKKEDE----------TKTKTEDALPQG 240

Query: 241 EDGNINKKEDDP------DHQQPKPATTTTTVEPTKPGEGGDQIIIMD--DHENMKRMMY 300
            D N NK+E+ P      +  +P   T  ++VE  KP EGG++ I+MD  D E MKRM+Y
Sbjct: 241 TDTNNNKEEEQPKPTEPAEPGEPGEETKASSVE-AKPAEGGEEKIVMDEVDPEIMKRMIY 300

Query: 301 H-YHYHPLYVIEPI-PPPQLFSDENPNACCI 312
           H Y Y PLYVIEPI PPPQLFSDENPNACCI
Sbjct: 301 HNYQYQPLYVIEPIPPPPQLFSDENPNACCI 313

BLAST of Tan0012852 vs. ExPASy TrEMBL
Match: A0A6J1JAB1 (heavy metal-associated isoprenylated plant protein 9 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111482690 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 5.7e-90
Identity = 213/337 (63.20%), Postives = 244/337 (72.40%), Query Frame = 0

Query: 1   MGEEAVKM-----EEPKVEETTKVEESK--PSPPFVLFVDLHCTGCAKKIEKSLMRIRGV 60
           MGEEAV+M     EE KVEE  K+EE+K  P PP VL VDLHC+GCAKKIEK +MRIRGV
Sbjct: 1   MGEEAVQMQQQQQEEAKVEE-MKLEEAKPCPCPPIVLLVDLHCSGCAKKIEKCIMRIRGV 60

Query: 61  EGVSIDMAKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPP-PQGEPIPQLL-NS 120
           EGVSIDMAKNEV+IKGI+   DP+A+CAKIT KTKR A+VLSP PP P+GEP P L+ NS
Sbjct: 61  EGVSIDMAKNEVTIKGIV---DPDAVCAKITTKTKRVAEVLSPSPPLPEGEPSPHLIVNS 120

Query: 121 QVGGLTLVELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYV 180
           Q   L +VELNVNMHC ACA QLKKKILKMRGVQTASTELSTGKV+VTGTMDGDKLV+YV
Sbjct: 121 Q---LKVVELNVNMHCDACAHQLKKKILKMRGVQTASTELSTGKVVVTGTMDGDKLVDYV 180

Query: 181 YRRTKKQARIVPQPEPAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIE- 240
           YRRTKKQARIVPQP+P   +      E+EP K  E+ KE+    PPPPPE  KT+     
Sbjct: 181 YRRTKKQARIVPQPQPTATS-----PEQEPLK-PEQSKEEQAAPPPPPPEEIKTEDATAP 240

Query: 241 ----EDGNINKKED------DPDHQQPKPATTTT----TVEPTKPGEGGDQIIIMDDHEN 300
                D N N KE+      +    Q   AT  T     VE  K  E GD+++  D+HE+
Sbjct: 241 QAQGTDANNNNKEEVQPKAAEEGGGQVVEATAETKPNVEVEEAKAAEAGDEMMGEDNHES 300

Query: 301 MKRMMY-HYHYHPLYVIEPIPPPQLFSDENPNACCIL 313
           MKRMMY +Y Y PLYV+E IPPPQLFSDENPNACC+L
Sbjct: 301 MKRMMYQYYQYQPLYVMERIPPPQLFSDENPNACCVL 324

BLAST of Tan0012852 vs. ExPASy TrEMBL
Match: A0A6J1J8F9 (heavy metal-associated isoprenylated plant protein 9 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111482690 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 1.1e-88
Identity = 213/337 (63.20%), Postives = 244/337 (72.40%), Query Frame = 0

Query: 1   MGEEAVKM---EEPKVEETTKVEESK--PSPPFVLFVDLHCTGCAKKIEKSLMRIRGVEG 60
           MGEEAV+M   EE KVEE  K+EE+K  P PP VL VDLHC+GCAKKIEK +MRIRGVEG
Sbjct: 1   MGEEAVQMQQQEEAKVEE-MKLEEAKPCPCPPIVLLVDLHCSGCAKKIEKCIMRIRGVEG 60

Query: 61  VSIDMAKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPP-PQGEPIPQLL-NSQV 120
           VSIDMAKNEV+IKGI+   DP+A+CAKIT KTKR A+VLSP PP P+GEP P L+ NSQ 
Sbjct: 61  VSIDMAKNEVTIKGIV---DPDAVCAKITTKTKRVAEVLSPSPPLPEGEPSPHLIVNSQ- 120

Query: 121 GGLTLVELNVNMHCQACALQLKKKILKMR--GVQTASTELSTGKVMVTGTMDGDKLVEYV 180
             L +VELNVNMHC ACA QLKKKILKMR  GVQTASTELSTGKV+VTGTMDGDKLV+YV
Sbjct: 121 --LKVVELNVNMHCDACAHQLKKKILKMRVSGVQTASTELSTGKVVVTGTMDGDKLVDYV 180

Query: 181 YRRTKKQARIVPQPEPAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIE- 240
           YRRTKKQARIVPQP+P   +      E+EP K  E+ KE+    PPPPPE  KT+     
Sbjct: 181 YRRTKKQARIVPQPQPTATS-----PEQEPLK-PEQSKEEQAAPPPPPPEEIKTEDATAP 240

Query: 241 ----EDGNINKKED------DPDHQQPKPATTTT----TVEPTKPGEGGDQIIIMDDHEN 300
                D N N KE+      +    Q   AT  T     VE  K  E GD+++  D+HE+
Sbjct: 241 QAQGTDANNNNKEEVQPKAAEEGGGQVVEATAETKPNVEVEEAKAAEAGDEMMGEDNHES 300

Query: 301 MKRMMY-HYHYHPLYVIEPIPPPQLFSDENPNACCIL 313
           MKRMMY +Y Y PLYV+E IPPPQLFSDENPNACC+L
Sbjct: 301 MKRMMYQYYQYQPLYVMERIPPPQLFSDENPNACCVL 324

BLAST of Tan0012852 vs. ExPASy TrEMBL
Match: A0A6J1JAA8 (heavy metal-associated isoprenylated plant protein 9 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482690 PE=4 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 1.4e-88
Identity = 213/338 (63.02%), Postives = 244/338 (72.19%), Query Frame = 0

Query: 1   MGEEAVKM----EEPKVEETTKVEESK--PSPPFVLFVDLHCTGCAKKIEKSLMRIRGVE 60
           MGEEAV+M    EE KVEE  K+EE+K  P PP VL VDLHC+GCAKKIEK +MRIRGVE
Sbjct: 1   MGEEAVQMQQQQEEAKVEE-MKLEEAKPCPCPPIVLLVDLHCSGCAKKIEKCIMRIRGVE 60

Query: 61  GVSIDMAKNEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPP-PQGEPIPQLL-NSQ 120
           GVSIDMAKNEV+IKGI+   DP+A+CAKIT KTKR A+VLSP PP P+GEP P L+ NSQ
Sbjct: 61  GVSIDMAKNEVTIKGIV---DPDAVCAKITTKTKRVAEVLSPSPPLPEGEPSPHLIVNSQ 120

Query: 121 VGGLTLVELNVNMHCQACALQLKKKILKMR--GVQTASTELSTGKVMVTGTMDGDKLVEY 180
              L +VELNVNMHC ACA QLKKKILKMR  GVQTASTELSTGKV+VTGTMDGDKLV+Y
Sbjct: 121 ---LKVVELNVNMHCDACAHQLKKKILKMRVSGVQTASTELSTGKVVVTGTMDGDKLVDY 180

Query: 181 VYRRTKKQARIVPQPEPAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIE 240
           VYRRTKKQARIVPQP+P   +      E+EP K  E+ KE+    PPPPPE  KT+    
Sbjct: 181 VYRRTKKQARIVPQPQPTATS-----PEQEPLK-PEQSKEEQAAPPPPPPEEIKTEDATA 240

Query: 241 -----EDGNINKKED------DPDHQQPKPATTTT----TVEPTKPGEGGDQIIIMDDHE 300
                 D N N KE+      +    Q   AT  T     VE  K  E GD+++  D+HE
Sbjct: 241 PQAQGTDANNNNKEEVQPKAAEEGGGQVVEATAETKPNVEVEEAKAAEAGDEMMGEDNHE 300

Query: 301 NMKRMMY-HYHYHPLYVIEPIPPPQLFSDENPNACCIL 313
           +MKRMMY +Y Y PLYV+E IPPPQLFSDENPNACC+L
Sbjct: 301 SMKRMMYQYYQYQPLYVMERIPPPQLFSDENPNACCVL 325

BLAST of Tan0012852 vs. TAIR 10
Match: AT5G24580.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 294.7 bits (753), Expect = 9.0e-80
Identity = 176/310 (56.77%), Postives = 215/310 (69.35%), Query Frame = 0

Query: 3   EEAVKMEEPKVEETTKVEESKPSPPFVLFVDLHCTGCAKKIEKSLMRIRGVEGVSIDMAK 62
           E+ V  EE K +E  + +   P PPF+L+VDLHC GCAKKIE+S+++IRGVE V +DM +
Sbjct: 33  EKKVAAEEEKPKEEEEPQPPPPPPPFILYVDLHCVGCAKKIERSILKIRGVEEVVMDMNE 92

Query: 63  NEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPIPQLLNSQV-GGLTLVEL 122
           N+V+IKG++   DP+A+C KI KKTKR AKVLSPLP  +GEP+P ++ SQV GGLT VEL
Sbjct: 93  NQVTIKGVL---DPQAVCNKIKKKTKRMAKVLSPLPAAEGEPLPPIITSQVSGGLTTVEL 152

Query: 123 NVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRTKKQARI 182
           +VNMHCQACA QLKKKILKMRGVQT  TE +TGKV+VTGTMD +KLV+YVYRRTKKQARI
Sbjct: 153 SVNMHCQACADQLKKKILKMRGVQTTVTEHTTGKVIVTGTMDAEKLVDYVYRRTKKQARI 212

Query: 183 VPQPEPAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIEEDGNINKKEDD 242
           VPQP+P         + E P    EEKKE+  +    PPE   T  E EE+    KKE +
Sbjct: 213 VPQPDP---------EPEAPAAAQEEKKEESGEGNEKPPE---TGEEKEEE---KKKEGE 272

Query: 243 PDHQQPKPATTTTTVEPTKPGEGGDQIIIMDDHENMKRMMYHYHYHPLYVIEPIPPPQLF 302
            + ++        T E  +  E    +  M   E MKRMM  Y+Y P YVIE IPPPQLF
Sbjct: 273 ENGEEGGGEEAAATEEERRDNE----MTAMAQEEGMKRMM--YYYQPSYVIERIPPPQLF 318

Query: 303 SDENPNACCI 312
           SDENPNACCI
Sbjct: 333 SDENPNACCI 318

BLAST of Tan0012852 vs. TAIR 10
Match: AT5G24580.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 294.7 bits (753), Expect = 9.0e-80
Identity = 176/310 (56.77%), Postives = 215/310 (69.35%), Query Frame = 0

Query: 3   EEAVKMEEPKVEETTKVEESKPSPPFVLFVDLHCTGCAKKIEKSLMRIRGVEGVSIDMAK 62
           E+ V  EE K +E  + +   P PPF+L+VDLHC GCAKKIE+S+++IRGVE V +DM +
Sbjct: 32  EKKVAAEEEKPKEEEEPQPPPPPPPFILYVDLHCVGCAKKIERSILKIRGVEEVVMDMNE 91

Query: 63  NEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPIPQLLNSQV-GGLTLVEL 122
           N+V+IKG++   DP+A+C KI KKTKR AKVLSPLP  +GEP+P ++ SQV GGLT VEL
Sbjct: 92  NQVTIKGVL---DPQAVCNKIKKKTKRMAKVLSPLPAAEGEPLPPIITSQVSGGLTTVEL 151

Query: 123 NVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRTKKQARI 182
           +VNMHCQACA QLKKKILKMRGVQT  TE +TGKV+VTGTMD +KLV+YVYRRTKKQARI
Sbjct: 152 SVNMHCQACADQLKKKILKMRGVQTTVTEHTTGKVIVTGTMDAEKLVDYVYRRTKKQARI 211

Query: 183 VPQPEPAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIEEDGNINKKEDD 242
           VPQP+P         + E P    EEKKE+  +    PPE   T  E EE+    KKE +
Sbjct: 212 VPQPDP---------EPEAPAAAQEEKKEESGEGNEKPPE---TGEEKEEE---KKKEGE 271

Query: 243 PDHQQPKPATTTTTVEPTKPGEGGDQIIIMDDHENMKRMMYHYHYHPLYVIEPIPPPQLF 302
            + ++        T E  +  E    +  M   E MKRMM  Y+Y P YVIE IPPPQLF
Sbjct: 272 ENGEEGGGEEAAATEEERRDNE----MTAMAQEEGMKRMM--YYYQPSYVIERIPPPQLF 317

Query: 303 SDENPNACCI 312
           SDENPNACCI
Sbjct: 332 SDENPNACCI 317

BLAST of Tan0012852 vs. TAIR 10
Match: AT5G24580.3 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 2.1e-76
Identity = 173/310 (55.81%), Postives = 212/310 (68.39%), Query Frame = 0

Query: 3   EEAVKMEEPKVEETTKVEESKPSPPFVLFVDLHCTGCAKKIEKSLMRIRGVEGVSIDMAK 62
           E+ V  EE K +E  + +   P PPF+L+VDLHC GCAKKIE+S+++IR    V +DM +
Sbjct: 33  EKKVAAEEEKPKEEEEPQPPPPPPPFILYVDLHCVGCAKKIERSILKIR---EVVMDMNE 92

Query: 63  NEVSIKGIIVDPDPEAICAKITKKTKRAAKVLSPLPPPQGEPIPQLLNSQV-GGLTLVEL 122
           N+V+IKG++   DP+A+C KI KKTKR AKVLSPLP  +GEP+P ++ SQV GGLT VEL
Sbjct: 93  NQVTIKGVL---DPQAVCNKIKKKTKRMAKVLSPLPAAEGEPLPPIITSQVSGGLTTVEL 152

Query: 123 NVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRTKKQARI 182
           +VNMHCQACA QLKKKILKMRGVQT  TE +TGKV+VTGTMD +KLV+YVYRRTKKQARI
Sbjct: 153 SVNMHCQACADQLKKKILKMRGVQTTVTEHTTGKVIVTGTMDAEKLVDYVYRRTKKQARI 212

Query: 183 VPQPEPAEINKPPEKDEEEPPKLAEEKKEDHDQEPPPPPEVTKTKTEIEEDGNINKKEDD 242
           VPQP+P         + E P    EEKKE+  +    PPE   T  E EE+    KKE +
Sbjct: 213 VPQPDP---------EPEAPAAAQEEKKEESGEGNEKPPE---TGEEKEEE---KKKEGE 272

Query: 243 PDHQQPKPATTTTTVEPTKPGEGGDQIIIMDDHENMKRMMYHYHYHPLYVIEPIPPPQLF 302
            + ++        T E  +  E    +  M   E MKRMM  Y+Y P YVIE IPPPQLF
Sbjct: 273 ENGEEGGGEEAAATEEERRDNE----MTAMAQEEGMKRMM--YYYQPSYVIERIPPPQLF 315

Query: 303 SDENPNACCI 312
           SDENPNACCI
Sbjct: 333 SDENPNACCI 315

BLAST of Tan0012852 vs. TAIR 10
Match: AT5G63530.1 (farnesylated protein 3 )

HSP 1 Score: 140.6 bits (353), Expect = 2.2e-33
Identity = 111/322 (34.47%), Postives = 168/322 (52.17%), Query Frame = 0

Query: 14  EETTKVEESKPSPP-----FVLFVDLHCTGCAKKIEKSLMRIRGVEGVSIDMAKNEVSIK 73
           E++       P+PP      VL V +HC GCA+K+ + L    GVE V  D    +V +K
Sbjct: 56  EDSAPAAPEAPAPPPPPQEVVLKVYMHCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVK 115

Query: 74  GIIVDPDPEAICAKITKKTKRAAKVLSPLPPP---------QGEPIPQLLNSQVGGLTLV 133
           G     DP  + A++ +KT R  ++LSP+PPP         + +PI +    +   +  V
Sbjct: 116 G--EKADPLKVLARVQRKTHRQVQLLSPIPPPPPPPEKKAEEDKPIVEEKKVEPPVVVTV 175

Query: 134 ELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRTKKQA 193
            L V+MHC+ACA ++KK+I++M+GV++A ++L + +V V G  +  KLVEYVY+RT K A
Sbjct: 176 VLKVHMHCEACATEIKKRIMRMKGVESAESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHA 235

Query: 194 RIVPQPEPAEINKPPEKDEEEPPKLAE-EKKEDHDQEPPPPPEVTK-TKTEIEEDGNINK 253
            I+      +I+ PP    EE    AE EKKE+   E     E  K  K + +E+    K
Sbjct: 236 AIM------KIDPPPPPPPEEAAAAAEGEKKEEEKGEGESKGEEGKDDKAKTDEE----K 295

Query: 254 KEDDPDHQQPKPATTTTTVEPTKPGEGGDQIIIMDDHENMKRMMYHYHYHPLYVIEP--- 313
           KE D    + + A             GG++   + +   ++   Y+Y+Y P  V  P   
Sbjct: 296 KEGDGGKGEGEAA----------DNGGGEEEGKVVEVRKIENPYYYYYYQPPRVAIPPME 355

BLAST of Tan0012852 vs. TAIR 10
Match: AT5G63530.2 (farnesylated protein 3 )

HSP 1 Score: 140.6 bits (353), Expect = 2.2e-33
Identity = 111/322 (34.47%), Postives = 168/322 (52.17%), Query Frame = 0

Query: 14  EETTKVEESKPSPP-----FVLFVDLHCTGCAKKIEKSLMRIRGVEGVSIDMAKNEVSIK 73
           E++       P+PP      VL V +HC GCA+K+ + L    GVE V  D    +V +K
Sbjct: 41  EDSAPAAPEAPAPPPPPQEVVLKVYMHCEGCARKVRRCLKGFEGVEDVMTDCKTGKVVVK 100

Query: 74  GIIVDPDPEAICAKITKKTKRAAKVLSPLPPP---------QGEPIPQLLNSQVGGLTLV 133
           G     DP  + A++ +KT R  ++LSP+PPP         + +PI +    +   +  V
Sbjct: 101 G--EKADPLKVLARVQRKTHRQVQLLSPIPPPPPPPEKKAEEDKPIVEEKKVEPPVVVTV 160

Query: 134 ELNVNMHCQACALQLKKKILKMRGVQTASTELSTGKVMVTGTMDGDKLVEYVYRRTKKQA 193
            L V+MHC+ACA ++KK+I++M+GV++A ++L + +V V G  +  KLVEYVY+RT K A
Sbjct: 161 VLKVHMHCEACATEIKKRIMRMKGVESAESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHA 220

Query: 194 RIVPQPEPAEINKPPEKDEEEPPKLAE-EKKEDHDQEPPPPPEVTK-TKTEIEEDGNINK 253
            I+      +I+ PP    EE    AE EKKE+   E     E  K  K + +E+    K
Sbjct: 221 AIM------KIDPPPPPPPEEAAAAAEGEKKEEEKGEGESKGEEGKDDKAKTDEE----K 280

Query: 254 KEDDPDHQQPKPATTTTTVEPTKPGEGGDQIIIMDDHENMKRMMYHYHYHPLYVIEP--- 313
           KE D    + + A             GG++   + +   ++   Y+Y+Y P  V  P   
Sbjct: 281 KEGDGGKGEGEAA----------DNGGGEEEGKVVEVRKIENPYYYYYYQPPRVAIPPME 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLU51.3e-7856.77Heavy metal-associated isoprenylated plant protein 9 OS=Arabidopsis thaliana OX=... [more]
Q9C5D33.0e-3234.47Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=... [more]
Q9SHQ82.0e-2028.48Heavy metal-associated isoprenylated plant protein 4 OS=Arabidopsis thaliana OX=... [more]
Q9FJH56.8e-1626.87Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=... [more]
Q9M8T77.5e-1530.10Heavy metal-associated isoprenylated plant protein 8 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
XP_023541673.13.5e-10270.82heavy metal-associated isoprenylated plant protein 9-like [Cucurbita pepo subsp.... [more]
XP_022968630.15.1e-10170.64heavy metal-associated isoprenylated plant protein 9-like [Cucurbita maxima][more]
XP_022944904.14.3e-10069.49heavy metal-associated isoprenylated plant protein 9-like [Cucurbita moschata] >... [more]
KAG7013337.14.7e-9968.56Heavy metal-associated isoprenylated plant protein 9 [Cucurbita argyrosperma sub... [more]
XP_023551857.19.6e-9264.39heavy metal-associated isoprenylated plant protein 9 isoform X3 [Cucurbita pepo ... [more]
Match NameE-valueIdentityDescription
A0A6J1HXR02.5e-10170.64heavy metal-associated isoprenylated plant protein 9-like OS=Cucurbita maxima OX... [more]
A0A6J1FZC72.1e-10069.49heavy metal-associated isoprenylated plant protein 9-like OS=Cucurbita moschata ... [more]
A0A6J1JAB15.7e-9063.20heavy metal-associated isoprenylated plant protein 9 isoform X3 OS=Cucurbita max... [more]
A0A6J1J8F91.1e-8863.20heavy metal-associated isoprenylated plant protein 9 isoform X4 OS=Cucurbita max... [more]
A0A6J1JAA81.4e-8863.02heavy metal-associated isoprenylated plant protein 9 isoform X2 OS=Cucurbita max... [more]
Match NameE-valueIdentityDescription
AT5G24580.19.0e-8056.77Heavy metal transport/detoxification superfamily protein [more]
AT5G24580.29.0e-8056.77Heavy metal transport/detoxification superfamily protein [more]
AT5G24580.32.1e-7655.81Heavy metal transport/detoxification superfamily protein [more]
AT5G63530.12.2e-3334.47farnesylated protein 3 [more]
AT5G63530.22.2e-3334.47farnesylated protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.70.100coord: 58..123
e-value: 7.2E-14
score: 54.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 132..188
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 121..209
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 64..110
e-value: 8.0E-9
score: 35.8
IPR006121Heavy metal-associated domain, HMAPROSITEPS50846HMA_2coord: 66..100
score: 9.075594
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 62..122
e-value: 1.35839E-6
score: 42.5929
IPR044258Heavy metal-associated isoprenylated plant protein 9-likePANTHERPTHR47066HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 9coord: 1..253
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 59..115

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0012852.1Tan0012852.1mRNA
Tan0012852.2Tan0012852.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding