Csor.00g000110 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g000110
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptiondual-specificity RNA methyltransferase RlmN
LocationCsor_Chr19: 8254760 .. 8258994 (-)
RNA-Seq ExpressionCsor.00g000110
SyntenyCsor.00g000110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCGTTTCGGTCTGCATTCGATGCCGGAGAGATGAGAACCGAGTTCGAGAAGGCCGGCATCAAATCGACCTTCATCCCCTTCATTTGGAAGCAGTTGCTTCATGATTCAAACAACCTATTGAACCAAAATATGGCATCCGAATTCCAATGGGAGAAAGCCGTGCCTTCGCTGCCATCGGCGGCTTACTCCTTGCTCCGGTCAAAGTTTAAGCCTCTTACTTCCACCGTCAATACCGTCGTCGATTCCTCCGATCAGGCCACCACCAAGCTCCTTATCAAGCTTCAGGTACCCTCCTCTTCCTTCCGCCCCCCCTTTCTCTTTTGGGAAAAGTTCGATGTTCACCCTAAATTTATCGGAATGAATCAATTTTTAACGTCAATTTCATCAACTAAAATACTGAACATAAATAAATTGGTGATAATACAAACCTATGCAGATTTTTCAAATCGTGTTTGTGGTTTTAATTAGTTGGGCCTAAGCACTTTATTGCTAAGGAGCGTTTACTATCTGCTTGATTGTGTGGAAATTCAAGTATTTTCCTGATATAATTTGAGCTTAACTCGTTTTTTTTTTTTTTTTTTTTTTTGGTTAGATGAATTGTGTGTACTTTTTTGGTAGGTTCGTTAGCAATTTTCAAAGTTCTATTAGATGAAACGGAAAGCTTTTAAAATCGACTCACCCACTTATGACTTGAGTTTATGGATAAATTTGAATTTCTCTCTTTTACTCTCATCCATCTGTGAAAGGTTTGGCGATTTAGACAATTTAATGATTATAGTTCAAATCAAGTAGTGGGTTTCTGTTGATTTCTGCAGTACTCTTCCGGTTATAATCGTTCTTTCAAATATCTTCGATGTTGTTAATCCTACCCCATGGCTTTATGCCTAGATCTTAGTTTTTTCCCCTCATGTTGTTAGAGTGGGGCTCTTGTGGAGGCTGTCATCATGAGGTATGATTCTCGTCTGGGAAAATATGGCGACAAACCTCGACCAGGTGGTCTGCGGTCTACTCTCTGTATTTCTTCGCAGGTTTTGATTTCTTCATCAGCATTGTCAGCATCCTCTGATTTTGTTTTTTTTTTTTTTTTTTTTGTTCTTGATATTTCGTTGTAAGTCTCACATTATATGTCTGTTCTCAGGTTGGCTGCAAAATGGGCTGCAGATTCTGTGCAACCGGAACAATGGGATACAAAAGCAATCTGACTTCAGGGGAGATAGTAGAGCAGTTGGTCCATGCCTCCCGTTTGTTCCAAATACGCAATGTTGTTTTCATGGTGATTCTTCCATCCTTTCATTTGTCTGAATTTTTATTGTATCAAAAAAAGTTTCCACTTAGAGGTAGACACTTTTGAGGCATCCCTATGGGATTTCATTTTTCTACTCAGCATGGCGTATGCATCCTTAGAGCGTATGCCCATATCATTCTTCGCCTAAACGTGAACATTCCTTGCTGTTCGAAACTTATTGACTTTGGATATGGCTTTAATGTACCACCCTTTCAAGAGTGCAAAAGCCTTACTATGAAAAAACTTCATCATGGCTATCTTTTGGAATCTATGGAAAGAAAGAAATGAGAGAATATTTGTAAAAAAGGCACCGAACTGCACAAAACTTTTCAACAATGTTGTTTACTAAGTTATATCTTGGTGTAAACTGCCTAATATTTTTACTTCCTATAGTTATGCCTCCCTCATTGTAAATTGGGAAGGTCTTTTATAAACACTACAGATAATGCATCCCTTTTGTAAATTGATAATACATCCCTTTTGTAAATTTCAATCATCAATGAAATTGTCTCGACTTTGAGGTTAATTTTGAAAATTGAATAATTTGCCAGAGAATTACGGTATTATGGACATATGTAGTGATTAATGGCAAAGTGTGCATGACAGGAACCAGACTACTGTTGCCTTATTTCGAGCATTCAACTTCATAAGCCCCTTCTCTTTATTTCTTAGCAGATTGTGGCCCCATGTCTGTTTAATTGTCCTCTGTTTCCTTGTTAGCAAGAAGTTGCCTGCCTCGTGCTTTCCTATCATTTCATACATTTTTTTTTTCTCTTAGAATATACTTTTTCATGTTGATTTTATTATTTTGTGACAGGGAATGGGAGAACCATTGAATAACTATTCTGCTTTAGTGGAAGCTGTCCGTGTCATGATAGGCTCCCCATTCTTATTATCACCCAAGAGAATTACTATCTCAACTGTATGTACTCTGATCTTCCTGTTTTCCATTTCTATGGTCTTTACTCTTTATCTAATTTTGTACGAATTTGAGGTCGTGCACTCATATAGTGCAATTCCTGCACGAATGTTTAGAACTACTAAGATCTTCTCCTTCATTCCTCTTGACAACGTCCAAATGTCATTATTTAAACTCAACATACTTTCGGATTATGTAAAAAGATGGATTTATCATTTGTTGTTAAGAAGTTTCAAGGCCGGAATTCATCAATCGATGAAACGTTTGTCTCTTGTCAAAGAAAAAAAGTTTCAAGGGCAGTGATCTTGTTATCTGAGAATAGTGTTTGTCCTAACGATGAGTCTTTTGTCTGAGATTCTTTATTTTTCTCAAGTCTGATCCTGAATATTTGATTATAAAATAAAGTAATGGGAGTTCAGAAAGTCAGTTTCTATCCTCTTATCCGTTTCCTCTTCCTTTGAGTTCTGTTAAACTGTTAAAAGAAGGTGAGTAGTCGAAGTTGTATTCTTTTGTATACCTGTAGGTTGGGATTATTCATGCTATAAGCAAACTGCATGGTGATCTACCGGGATTGAACTTGGCCATTTCCTTGCACGCACCTGCGCAAGATATTCGTTGCCAGATAATGCCTGCAGCTCGGGCTTTTCCTTTACAAAAACTTATGGATGCTCTCCAAGAATATCAACAAAAGAGGTAAATACGTTGTATTTGCTTCTGCTGAACATGTCGTGATACTCCAACGCTTCTCCAATTTAAATGTATGTTATGTCTATTGTTTCAGTCAGCAAAAGATATTCATTGAGTACATAATGCTTGACGGAGTGAATGATGAAGAGCAACATGCCCACCAACTTGGAAAGTTGTTAGAGACATTCCTGGTGGTAAGCTCTCAATATAGATCATGGTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACAATTTTATTGATAATTGAAATTTACAGAAGGGATGTATAATTCATGTTGTTTACAAAAGAACTTCCCAATTTTCCACGAGAAGTATAACTATAGGAAATAAAAATATTAGACAGGTTACACCAAGATATAGCTTGGTAAACAACATTGTCGAAAAGTTTTGCATAGGTACGTGTCTTTTCTGCAAACATTCTCTGATTTCTTTCTTTCCACATATTCCAAAATAAAACCATGATGAGGTTTTTCCATAATAGGGCTTTTACATTCTTGAAAGGGTGGTACGTTAAAGGTCATATCCAAAAATTCCGATGTCATTCGAATATATTAAGAATCGTTGTCTAGAAACTCTGAGCGTATGTGCATTGCATAAACAAGTGACTCTGCTTGCAAGTGGCTTTGAGAATCGTTTATCATGATTTTTTGTGTGTTTGAAAGTTTCTTGGAGACTTCACACGTAATCACCCCATGAATCTAGTACCTTTGTTTGCTTAGCAATTTTGATGTTACTAAATTGTACCTTCCTGGAAAAACAGGTTGTGAACTTAATACCATTCAATCCTATTGGTAGTTCAAGCCAATTCAAAACCAGTAGTGATGAGAAAGTTTCAGCATTCCAACAAGTTCTTCGGGGTACGTATAATATACGCACAACCATTCGTAAGCAGATGGGTCAAGATATTAGTGGGGCATGTGGTCAACTGGTGGTCAGCCAAACTAATAGAATCTCCGGTAAACAAACAACTGTTCTACCTGACATCGAAGATCTTCACCTCTGATTTAGACGAGATACCACCAGGTGAGTTGCGCGTCGTGTGCCTATTTTAGACGAGAATGATGATGTAACTTGGGTTGATATTATCTTCAGATGTTTACTAGTTTGTCTTCGGGTCCAAATTCGACTGGAATCCACTGTTGTATGCTGTCAACTTTTGTTTCTTTTAATTTTGATTTGTTTGCCATAGATCTTAGTATCTTTTTCTGCACTTTCTTCTGTTTCAGCTGTTGGTGGTTGGTTGGACGAGTCGATCAGCCTTGGGAGGGGGATCGAAATCCTTGGACGACATGAAAATGCCAAGAAAACAGAGAAGTAA

mRNA sequence

ATGGCGGCGTTTCGGTCTGCATTCGATGCCGGAGAGATGAGAACCGAGTTCGAGAAGGCCGGCATCAAATCGACCTTCATCCCCTTCATTTGGAAGCAGTTGCTTCATGATTCAAACAACCTATTGAACCAAAATATGGCATCCGAATTCCAATGGGAGAAAGCCGTGCCTTCGCTGCCATCGGCGGCTTACTCCTTGCTCCGGTCAAAGTTTAAGCCTCTTACTTCCACCGTCAATACCGTCGTCGATTCCTCCGATCAGGCCACCACCAAGCTCCTTATCAAGCTTCAGAGTGGGGCTCTTGTGGAGGCTGTCATCATGAGGTATGATTCTCGTCTGGGAAAATATGGCGACAAACCTCGACCAGGTGGTCTGCGGTCTACTCTCTGTATTTCTTCGCAGGTTGGCTGCAAAATGGGCTGCAGATTCTGTGCAACCGGAACAATGGGATACAAAAGCAATCTGACTTCAGGGGAGATAGTAGAGCAGTTGGTCCATGCCTCCCGTTTGTTCCAAATACGCAATGTTGTTTTCATGGGAATGGGAGAACCATTGAATAACTATTCTGCTTTAGTGGAAGCTGTCCGTGTCATGATAGGCTCCCCATTCTTATTATCACCCAAGAGAATTACTATCTCAACTGTTGGGATTATTCATGCTATAAGCAAACTGCATGGTGATCTACCGGGATTGAACTTGGCCATTTCCTTGCACGCACCTGCGCAAGATATTCGTTGCCAGATAATGCCTGCAGCTCGGGCTTTTCCTTTACAAAAACTTATGGATGCTCTCCAAGAATATCAACAAAAGAGTCAGCAAAAGATATTCATTGAGTACATAATGCTTGACGGAGTGAATGATGAAGAGCAACATGCCCACCAACTTGGAAAGTTGTTAGAGACATTCCTGGTGGTTGTGAACTTAATACCATTCAATCCTATTGGTAGTTCAAGCCAATTCAAAACCAGTAGTGATGAGAAAGTTTCAGCATTCCAACAAGTTCTTCGGGCCAAACTAATAGAATCTCCGGTAAACAAACAACTGTTCTACCTGACATCGAAGATCTTCACCTCTGATTTAGACGAGATACCACCAGCTGTTGGTGGTTGGTTGGACGAGTCGATCAGCCTTGGGAGGGGGATCGAAATCCTTGGACGACATGAAAATGCCAAGAAAACAGAGAAGTAA

Coding sequence (CDS)

ATGGCGGCGTTTCGGTCTGCATTCGATGCCGGAGAGATGAGAACCGAGTTCGAGAAGGCCGGCATCAAATCGACCTTCATCCCCTTCATTTGGAAGCAGTTGCTTCATGATTCAAACAACCTATTGAACCAAAATATGGCATCCGAATTCCAATGGGAGAAAGCCGTGCCTTCGCTGCCATCGGCGGCTTACTCCTTGCTCCGGTCAAAGTTTAAGCCTCTTACTTCCACCGTCAATACCGTCGTCGATTCCTCCGATCAGGCCACCACCAAGCTCCTTATCAAGCTTCAGAGTGGGGCTCTTGTGGAGGCTGTCATCATGAGGTATGATTCTCGTCTGGGAAAATATGGCGACAAACCTCGACCAGGTGGTCTGCGGTCTACTCTCTGTATTTCTTCGCAGGTTGGCTGCAAAATGGGCTGCAGATTCTGTGCAACCGGAACAATGGGATACAAAAGCAATCTGACTTCAGGGGAGATAGTAGAGCAGTTGGTCCATGCCTCCCGTTTGTTCCAAATACGCAATGTTGTTTTCATGGGAATGGGAGAACCATTGAATAACTATTCTGCTTTAGTGGAAGCTGTCCGTGTCATGATAGGCTCCCCATTCTTATTATCACCCAAGAGAATTACTATCTCAACTGTTGGGATTATTCATGCTATAAGCAAACTGCATGGTGATCTACCGGGATTGAACTTGGCCATTTCCTTGCACGCACCTGCGCAAGATATTCGTTGCCAGATAATGCCTGCAGCTCGGGCTTTTCCTTTACAAAAACTTATGGATGCTCTCCAAGAATATCAACAAAAGAGTCAGCAAAAGATATTCATTGAGTACATAATGCTTGACGGAGTGAATGATGAAGAGCAACATGCCCACCAACTTGGAAAGTTGTTAGAGACATTCCTGGTGGTTGTGAACTTAATACCATTCAATCCTATTGGTAGTTCAAGCCAATTCAAAACCAGTAGTGATGAGAAAGTTTCAGCATTCCAACAAGTTCTTCGGGCCAAACTAATAGAATCTCCGGTAAACAAACAACTGTTCTACCTGACATCGAAGATCTTCACCTCTGATTTAGACGAGATACCACCAGCTGTTGGTGGTTGGTTGGACGAGTCGATCAGCCTTGGGAGGGGGATCGAAATCCTTGGACGACATGAAAATGCCAAGAAAACAGAGAAGTAA

Protein sequence

MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLPSAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKPRPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMGMGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAPAQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLETFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKLIESPVNKQLFYLTSKIFTSDLDEIPPAVGGWLDESISLGRGIEILGRHENAKKTEK
Homology
BLAST of Csor.00g000110 vs. ExPASy Swiss-Prot
Match: A0LQM1 (Dual-specificity RNA methyltransferase RlmN OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=rlmN PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 3.5e-49
Identity = 112/270 (41.48%), Postives = 167/270 (61.85%), Query Frame = 0

Query: 83  DSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKPRPGGLRSTLCISSQVGCKMGCR 142
           + +D  T+K L  L+ G  +EAV++              P   RSTLC+SSQVGC +GC+
Sbjct: 72  EQADDGTSKYLFGLRDGHSIEAVLI--------------PDLPRSTLCVSSQVGCALGCK 131

Query: 143 FCATGTMGYKSNLTSGEIVEQLVHASR----LFQIRNVVFMGMGEPLNNYSALVEAVRVM 202
           FC TG++G+K NL++ EIV+Q+    R      +I N+VFMGMGEPL N  +++ A+RV 
Sbjct: 132 FCLTGSLGFKRNLSAAEIVDQVCQVQRDLGSRSRITNIVFMGMGEPLANLDSVLRAIRV- 191

Query: 203 IGSP--FLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAPAQDIRCQIMPAARAFP 262
           I  P     S +RIT+ST G++  + +L  + P +NLA+SLHA   ++R ++MP  R +P
Sbjct: 192 IAEPNGMAFSHRRITLSTAGLVPQLRRLGRESP-VNLAVSLHAAENELRAELMPVNRTYP 251

Query: 263 LQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLETFLVVVNLIPFNPIGS 322
           L+ LM A +EY    +++I  EYI+LDG+ND+ + A QL KLL      VNL+PFNP   
Sbjct: 252 LEVLMAACREYPLPPRKRITFEYILLDGINDDPKQAKQLVKLLHGIRAKVNLMPFNP-HP 311

Query: 323 SSQFKTSSDEKVSAFQQVLRAKLIESPVNK 347
            S F+  S+++V AFQ+ L+   I + V +
Sbjct: 312 GSVFRKPSEQRVLAFQEALQNARITTHVRR 324

BLAST of Csor.00g000110 vs. ExPASy Swiss-Prot
Match: Q1DCU1 (Dual-specificity RNA methyltransferase RlmN 1 OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=rlmN1 PE=3 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 5.9e-49
Identity = 113/271 (41.70%), Postives = 162/271 (59.78%), Query Frame = 0

Query: 84  SSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKPRPGGLRSTLCISSQVGCKMGCRF 143
           SSD  T KLL++L  G  +E V+MR+                R+T+CIS+Q GC MGC F
Sbjct: 77  SSDGFTHKLLLRLDDGQTIETVLMRFKG--------------RATVCISTQAGCAMGCVF 136

Query: 144 CATGTMGYKSNLTSGEIVEQLVHASRLFQ-----IRNVVFMGMGEPLNNYSALVEAVRVM 203
           CATG MG   +LT GEIV Q++H +R+ +     +RNVV MGMGEPL+NY   + AV V+
Sbjct: 137 CATGQMGLSRHLTPGEIVGQILHVNRILRASGETLRNVVLMGMGEPLHNYEHTMSAVDVL 196

Query: 204 IGSPFL-LSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAPAQDIRCQIMPAARAFPL 263
           + +  L + P+ IT+STVG++  I +L  +   ++LA+SLH      R  ++PA R +PL
Sbjct: 197 VDALGLAMGPRFITLSTVGVVPGIRRLADEERPIHLAVSLHGATDAERAALVPAGRRWPL 256

Query: 264 QKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLETFLVVVNLIPFNP-IGS 323
            +LMDA + Y +K +++IF E+ ++ G ND  +HAH LG+LL      VN+IP NP +G 
Sbjct: 257 DELMDACRYYSEKRKRRIFFEWTLISGRNDTAEHAHTLGQLLRGMDAHVNVIPLNPTVGY 316

Query: 324 SSQFKTSSDEKVSAFQQVLRAKLIESPVNKQ 348
                 S  E V AFQ VL    + S V ++
Sbjct: 317 DG--GPSRPESVRAFQDVLATYDVPSTVRQR 331

BLAST of Csor.00g000110 vs. ExPASy Swiss-Prot
Match: A9A3L9 (Ribosomal RNA large subunit methyltransferase N OS=Nitrosopumilus maritimus (strain SCM1) OX=436308 GN=rlmN PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.0e-48
Identity = 113/292 (38.70%), Postives = 177/292 (60.62%), Query Frame = 0

Query: 56  VPSLPSAAYSLLRSKFKPLTSTVNTV-----VDSSDQATTKLLIKLQSGALVEAVIMRYD 115
           +P LP      LR +F     T+ +      V S D  TTKLL++L  G+ VE V+M+Y+
Sbjct: 44  IPQLPKK----LREEFTEAGYTIGSAKEIHRVVSDDGDTTKLLLELSDGSSVETVLMQYE 103

Query: 116 SRLGKYGDKPRPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRL 175
               K G  P     RST+C+S+Q+GC MGC FCATG MG+++NL +  IV Q++H + L
Sbjct: 104 PT--KIGGHP-----RSTICVSTQIGCAMGCVFCATGQMGFETNLKAEHIVSQVIHFAEL 163

Query: 176 FQ-----IRNVVFMGMGEPLNNYSALVEAVRVMI-GSPFLLSPKRITISTVGIIHAISKL 235
            +     + N+VFMGMGEP+ NY  ++ AV+++     F L  + ITIST+GI   I KL
Sbjct: 164 LEQRGEHVTNLVFMGMGEPMANYDEMIRAVKILTHDRGFGLGQRHITISTIGITSGIEKL 223

Query: 236 HGDLPGLNLAISLHAPAQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDG 295
             +   + LAISLHAP  ++R +++P A    ++ ++ + ++Y +K+ +++  EY +++G
Sbjct: 224 AEENLQIGLAISLHAPNNELRKKLVPTAGPNSVEDIIKSGRDYFKKTGRRVTFEYALMEG 283

Query: 296 VNDEEQHAHQLGKLLETFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLR 337
           VND  + AH+L +LL      VN+IP NP  ++  FK  S++ V  F+Q+LR
Sbjct: 284 VNDSPEIAHELARLLRGNGSHVNIIPINP--TAGDFKRPSEKNVLEFEQILR 322

BLAST of Csor.00g000110 vs. ExPASy Swiss-Prot
Match: Q6MDD0 (Probable dual-specificity RNA methyltransferase RlmN 2 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=rlmN2 PE=3 SV=2)

HSP 1 Score: 191.4 bits (485), Expect = 1.9e-47
Identity = 111/290 (38.28%), Postives = 170/290 (58.62%), Query Frame = 0

Query: 52  WEKAVPSLPSAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDS 111
           W+K      S    L +    P+   V    +S DQ T K L +L+ G LVE+V++    
Sbjct: 40  WDKMSNLSQSLREKLAKHIRLPVLELVR-YTESIDQETIKFLWRLRDGNLVESVLIL--- 99

Query: 112 RLGKYGDKPRPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVH----- 171
                       G+R T+C+SSQVGC   C FCA+G  G+  NL   EI+EQ++      
Sbjct: 100 -----------SGIRRTVCVSSQVGCPAKCAFCASGQQGFFRNLRPTEIIEQILQINAWL 159

Query: 172 ASRLFQIRNVVFMGMGEPLNNYSALVEAVRVMIGSPFL-LSPKRITISTVGIIHAISKLH 231
           +S+  ++ +VV+MGMGEPL NY ++V ++RV+    F  +S +RIT+STVG++  I +L 
Sbjct: 160 SSKGEKVSHVVYMGMGEPLKNYESVVASIRVLSHPDFCNISQRRITVSTVGVVEGIKRLS 219

Query: 232 GDLPGLNLAISLHAPAQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGV 291
            +   +NL +SLHAP Q IR +I+P AR +PL+++++++ EY QK+++ I  EY +L G+
Sbjct: 220 KEGLKVNLVLSLHAPNQHIRKKIIPYARKYPLEEILESMDEYAQKTKRDITFEYTLLAGI 279

Query: 292 NDEEQHAHQLGKLLETFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVL 336
           ND   HAH+L  LL+     VNLIP+NPI    + K    + +  F+ VL
Sbjct: 280 NDHPDHAHELAHLLKGKQCTVNLIPYNPI-PGLRLKRPEKKAIKQFRSVL 313

BLAST of Csor.00g000110 vs. ExPASy Swiss-Prot
Match: Q1MQJ3 (Dual-specificity RNA methyltransferase RlmN OS=Lawsonia intracellularis (strain PHE/MN1-00) OX=363253 GN=rlmN PE=3 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 6.1e-46
Identity = 121/314 (38.54%), Postives = 177/314 (56.37%), Query Frame = 0

Query: 49  EFQWEK------AVPSLPSAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALV 108
           ++ W+K      ++ +LP    + L +  K     + T+  SSD  T K L++L  GAL+
Sbjct: 32  QWVWQKQITDIESMTNLPQKIRASLTALIKINLPEIVTIQQSSD-GTKKFLLRLSDGALI 91

Query: 109 EAVIMRYDSRLGKYGDKPRPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVE 168
           E V++             + G +R T C+SSQVGC MGC FC+T TMG+  NLT+GEIV 
Sbjct: 92  ETVLI---------PSIDKAGNIRITQCLSSQVGCSMGCTFCSTATMGFIRNLTAGEIVS 151

Query: 169 QLVHASRLFQ--------IRNVVFMGMGEPLNNYSALVEAVRVMIGSPFL-LSPKRITIS 228
           Q++ A             IRN+VFMGMGEPL N + L  A+ ++     L  S +RIT+S
Sbjct: 152 QVLLAKLHLNDNKPDKPIIRNIVFMGMGEPLLNLTELTRALHILHSEKGLNFSARRITVS 211

Query: 229 TVGIIHAISKLHGDLPGLN-LAISLHAPAQDIRCQIMPAARAFPLQKLMDALQEYQQKSQ 288
           T GI   I  L  +  GL  LA+SLHA  Q++R  IMP A  + L++L+D L+ Y  K +
Sbjct: 212 TCGIKKGIQALSEN--GLAFLALSLHASNQELRSTIMPKAAKWDLKELIDTLKNYSLKKR 271

Query: 289 QKIFIEYIMLDGVNDEEQHAHQLGKLLETFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQ 347
           +KI  EY++L G+ND  +HA +L KL+      +NLIP+NP       K  ++E +  FQ
Sbjct: 272 EKITFEYLLLGGINDSPEHAKELAKLITDIKGKLNLIPYNPAQGQPYLK-PTEENILKFQ 331

BLAST of Csor.00g000110 vs. NCBI nr
Match: KAG6571920.1 (hypothetical protein SDJN03_28648, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 771 bits (1991), Expect = 7.43e-281
Identity = 395/395 (100.00%), Postives = 395/395 (100.00%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60
           MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP
Sbjct: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60

Query: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120
           SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP
Sbjct: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180

Query: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP 240
           MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP
Sbjct: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP 240

Query: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300
           AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKLIESPVNKQLFYLTSKIFTSDL 360
           TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKLIESPVNKQLFYLTSKIFTSDL
Sbjct: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKLIESPVNKQLFYLTSKIFTSDL 360

Query: 361 DEIPPAVGGWLDESISLGRGIEILGRHENAKKTEK 395
           DEIPPAVGGWLDESISLGRGIEILGRHENAKKTEK
Sbjct: 361 DEIPPAVGGWLDESISLGRGIEILGRHENAKKTEK 395

BLAST of Csor.00g000110 vs. NCBI nr
Match: XP_023553823.1 (uncharacterized protein LOC111811282 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 652 bits (1682), Expect = 5.94e-234
Identity = 336/349 (96.28%), Postives = 340/349 (97.42%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60
           MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP
Sbjct: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60

Query: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120
           SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGA VEAVIMRYDSRLGKYGDKP
Sbjct: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGAFVEAVIMRYDSRLGKYGDKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180

Query: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP 240
           MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLA+SLHAP
Sbjct: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAVSLHAP 240

Query: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300
           AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKL-IESPVNKQL 348
           TF VVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLR    I + + KQ+
Sbjct: 301 TFQVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRGTYNIRTTIRKQM 349

BLAST of Csor.00g000110 vs. NCBI nr
Match: XP_022972434.1 (uncharacterized protein LOC111470995 [Cucurbita maxima])

HSP 1 Score: 651 bits (1680), Expect = 1.20e-233
Identity = 335/349 (95.99%), Postives = 339/349 (97.13%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60
           MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP
Sbjct: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60

Query: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120
           SA YSLLRSKFKPLTSTVNTVVDSSDQ TTKLLIKLQSGA VEAVIMRYDSRLGKYGDKP
Sbjct: 61  SATYSLLRSKFKPLTSTVNTVVDSSDQTTTKLLIKLQSGAFVEAVIMRYDSRLGKYGDKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180

Query: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP 240
           MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLA+SLHAP
Sbjct: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAVSLHAP 240

Query: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300
           AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKL-IESPVNKQL 348
           TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLR    I + + KQ+
Sbjct: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRGTYNIRTTIRKQM 349

BLAST of Csor.00g000110 vs. NCBI nr
Match: XP_022953081.1 (uncharacterized protein LOC111455593 [Cucurbita moschata])

HSP 1 Score: 650 bits (1678), Expect = 2.41e-233
Identity = 335/349 (95.99%), Postives = 341/349 (97.71%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60
           MAAFRSAFDAGEMRTEFEKAGIKSTFIP IWKQL+HDSNNLLNQNMASEFQWEKAVPSLP
Sbjct: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPSIWKQLIHDSNNLLNQNMASEFQWEKAVPSLP 60

Query: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120
           SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP
Sbjct: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180

Query: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP 240
           MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLA+SLHAP
Sbjct: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAVSLHAP 240

Query: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300
           AQDIRCQIMPAARAFPLQKLMDALQEYQQKS+QKIFIEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSRQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKL-IESPVNKQL 348
           TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLR    I + + KQ+
Sbjct: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRGTYNIRTTIRKQM 349

BLAST of Csor.00g000110 vs. NCBI nr
Match: XP_038888929.1 (dual-specificity RNA methyltransferase RlmN [Benincasa hispida])

HSP 1 Score: 606 bits (1562), Expect = 1.11e-215
Identity = 308/349 (88.25%), Postives = 325/349 (93.12%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60
           MAAFRSAFDAGEMRTEFEKAG+K+TFIPFIWK LLHD NNL N N+ASEF+WE+AVPSLP
Sbjct: 1   MAAFRSAFDAGEMRTEFEKAGVKTTFIPFIWKHLLHDPNNLFNHNLASEFEWEQAVPSLP 60

Query: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120
           SAAY LLRSKFKPLTSTV++VVDSSDQ TTKLLIKLQSGA VEAVIMRYD+RLGKYG KP
Sbjct: 61  SAAYCLLRSKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180
           RPGG RSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHAS L QIRN+VFMG
Sbjct: 121 RPGGPRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASHLSQIRNIVFMG 180

Query: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP 240
           MGEPLNNYSALVEAVR MIGSPFLLSPKRITISTVGIIHAI+KLHGDLPGLNLA+SLHAP
Sbjct: 181 MGEPLNNYSALVEAVRAMIGSPFLLSPKRITISTVGIIHAINKLHGDLPGLNLAVSLHAP 240

Query: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300
            QDIRCQIMPAARAFPLQKLMDALQEYQ+KSQQKI IEYIMLDGVND+EQHAHQLGKLLE
Sbjct: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQKKSQQKILIEYIMLDGVNDQEQHAHQLGKLLE 300

Query: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKL-IESPVNKQL 348
           TF VVVNLIPFNPIGSSSQF+TS DEKVS FQ+VLR    I + V KQ+
Sbjct: 301 TFQVVVNLIPFNPIGSSSQFRTSDDEKVSVFQKVLRGTYNIRTTVRKQM 349

BLAST of Csor.00g000110 vs. ExPASy TrEMBL
Match: A0A6J1IBJ2 (uncharacterized protein LOC111470995 OS=Cucurbita maxima OX=3661 GN=LOC111470995 PE=4 SV=1)

HSP 1 Score: 651 bits (1680), Expect = 5.80e-234
Identity = 335/349 (95.99%), Postives = 339/349 (97.13%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60
           MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP
Sbjct: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60

Query: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120
           SA YSLLRSKFKPLTSTVNTVVDSSDQ TTKLLIKLQSGA VEAVIMRYDSRLGKYGDKP
Sbjct: 61  SATYSLLRSKFKPLTSTVNTVVDSSDQTTTKLLIKLQSGAFVEAVIMRYDSRLGKYGDKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180

Query: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP 240
           MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLA+SLHAP
Sbjct: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAVSLHAP 240

Query: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300
           AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKL-IESPVNKQL 348
           TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLR    I + + KQ+
Sbjct: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRGTYNIRTTIRKQM 349

BLAST of Csor.00g000110 vs. ExPASy TrEMBL
Match: A0A6J1GM04 (uncharacterized protein LOC111455593 OS=Cucurbita moschata OX=3662 GN=LOC111455593 PE=4 SV=1)

HSP 1 Score: 650 bits (1678), Expect = 1.17e-233
Identity = 335/349 (95.99%), Postives = 341/349 (97.71%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60
           MAAFRSAFDAGEMRTEFEKAGIKSTFIP IWKQL+HDSNNLLNQNMASEFQWEKAVPSLP
Sbjct: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPSIWKQLIHDSNNLLNQNMASEFQWEKAVPSLP 60

Query: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120
           SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP
Sbjct: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180

Query: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP 240
           MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLA+SLHAP
Sbjct: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAVSLHAP 240

Query: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300
           AQDIRCQIMPAARAFPLQKLMDALQEYQQKS+QKIFIEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSRQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKL-IESPVNKQL 348
           TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLR    I + + KQ+
Sbjct: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRGTYNIRTTIRKQM 349

BLAST of Csor.00g000110 vs. ExPASy TrEMBL
Match: A0A0A0K4F5 (Radical_SAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G071380 PE=4 SV=1)

HSP 1 Score: 629 bits (1622), Expect = 1.23e-224
Identity = 318/366 (86.89%), Postives = 338/366 (92.35%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60
           MA FRSAFDAGE+RTEFEKAGIK+TFIPFIWK LLHD NN+ N N+ASEFQWE+ VPSLP
Sbjct: 1   MAVFRSAFDAGELRTEFEKAGIKTTFIPFIWKHLLHDPNNIFNHNLASEFQWEQVVPSLP 60

Query: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120
           SAAYSLLR KFKPLTSTV++VVDSSDQ TTKLLIKLQSGA VEAVIMRYD+RLGKYG KP
Sbjct: 61  SAAYSLLRLKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMG+KSNLTSGEIVEQLVHASR  QIRN+VFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEIVEQLVHASRFSQIRNIVFMG 180

Query: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP 240
           MGEPLNNYS+LVEAVR M+  PFLLSPKRITISTVGIIHAI+KLH DLPGLNLA+SLHAP
Sbjct: 181 MGEPLNNYSSLVEAVRAMMAPPFLLSPKRITISTVGIIHAINKLHSDLPGLNLAVSLHAP 240

Query: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300
            QDIRCQIMPAARAFPLQKLMDALQEYQ+KSQQKI IEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQKKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKLIESPVNKQLFYLTSKIFTSDL 360
           TF V+VNLIPFNPIGSSSQFKTSSDEKVS FQQVLRA LIE+ VN  + YLT KIFTSD+
Sbjct: 301 TFQVIVNLIPFNPIGSSSQFKTSSDEKVSVFQQVLRACLIEAQVNNLVLYLTLKIFTSDV 360

Query: 361 DEIPPA 366
            +IP A
Sbjct: 361 HKIPSA 366

BLAST of Csor.00g000110 vs. ExPASy TrEMBL
Match: A0A5D3C8Y4 (Dual-specificity RNA methyltransferase RlmN OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001230 PE=4 SV=1)

HSP 1 Score: 599 bits (1544), Expect = 2.96e-213
Identity = 302/349 (86.53%), Postives = 322/349 (92.26%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60
           MA FRSAFDAGE+RTEFEKAGIK+TFIPFIWK LLHD NNL N N+ASEFQWE+AVPSLP
Sbjct: 1   MAVFRSAFDAGELRTEFEKAGIKTTFIPFIWKHLLHDQNNLFNHNLASEFQWEQAVPSLP 60

Query: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120
           SAAY LLR KFKPLTSTV++VVDSSDQ TTKLLIKLQSGA VEAVIMRYD+RLGKYG KP
Sbjct: 61  SAAYCLLRLKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMG+KSNLTSGEIVEQL+HASR  QIRN+VFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEIVEQLIHASRFSQIRNIVFMG 180

Query: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP 240
           MGEPLNNYS+LVEAVR M+G PFLLSPKRITISTVGIIHAI+KLH DLPGLNLA+SLHAP
Sbjct: 181 MGEPLNNYSSLVEAVRAMMGPPFLLSPKRITISTVGIIHAINKLHSDLPGLNLAVSLHAP 240

Query: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300
            QDIRCQIMPAARAFPLQKLMDALQEYQ+KSQQKI IEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQKKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKL-IESPVNKQL 348
           TF V+VNLIPFNPIGSSSQFKTS DEKVS FQ++LR    I + V KQ+
Sbjct: 301 TFQVIVNLIPFNPIGSSSQFKTSGDEKVSVFQKILRGTYNIRTTVRKQM 349

BLAST of Csor.00g000110 vs. ExPASy TrEMBL
Match: A0A1S3C167 (dual-specificity RNA methyltransferase RlmN OS=Cucumis melo OX=3656 GN=LOC103495308 PE=4 SV=1)

HSP 1 Score: 599 bits (1544), Expect = 2.96e-213
Identity = 302/349 (86.53%), Postives = 322/349 (92.26%), Query Frame = 0

Query: 1   MAAFRSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLP 60
           MA FRSAFDAGE+RTEFEKAGIK+TFIPFIWK LLHD NNL N N+ASEFQWE+AVPSLP
Sbjct: 1   MAVFRSAFDAGELRTEFEKAGIKTTFIPFIWKHLLHDQNNLFNHNLASEFQWEQAVPSLP 60

Query: 61  SAAYSLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKP 120
           SAAY LLR KFKPLTSTV++VVDSSDQ TTKLLIKLQSGA VEAVIMRYD+RLGKYG KP
Sbjct: 61  SAAYCLLRLKFKPLTSTVHSVVDSSDQVTTKLLIKLQSGAFVEAVIMRYDTRLGKYGGKP 120

Query: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMG 180
           RPGGLRSTLCISSQVGCKMGCRFCATGTMG+KSNLTSGEIVEQL+HASR  QIRN+VFMG
Sbjct: 121 RPGGLRSTLCISSQVGCKMGCRFCATGTMGFKSNLTSGEIVEQLIHASRFSQIRNIVFMG 180

Query: 181 MGEPLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAP 240
           MGEPLNNYS+LVEAVR M+G PFLLSPKRITISTVGIIHAI+KLH DLPGLNLA+SLHAP
Sbjct: 181 MGEPLNNYSSLVEAVRAMMGPPFLLSPKRITISTVGIIHAINKLHSDLPGLNLAVSLHAP 240

Query: 241 AQDIRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLE 300
            QDIRCQIMPAARAFPLQKLMDALQEYQ+KSQQKI IEYIMLDGVNDEEQHAHQLGKLLE
Sbjct: 241 VQDIRCQIMPAARAFPLQKLMDALQEYQKKSQQKILIEYIMLDGVNDEEQHAHQLGKLLE 300

Query: 301 TFLVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKL-IESPVNKQL 348
           TF V+VNLIPFNPIGSSSQFKTS DEKVS FQ++LR    I + V KQ+
Sbjct: 301 TFQVIVNLIPFNPIGSSSQFKTSGDEKVSVFQKILRGTYNIRTTVRKQM 349

BLAST of Csor.00g000110 vs. TAIR 10
Match: AT3G19630.1 (Radical SAM superfamily protein )

HSP 1 Score: 474.6 bits (1220), Expect = 8.0e-134
Identity = 240/345 (69.57%), Postives = 289/345 (83.77%), Query Frame = 0

Query: 5   RSAFDAGEMRTEFEKAGIKSTFIPFIWKQLLHDSNNLLNQNMASEFQWEKAVPSLPSAAY 64
           +S FDA E+++EFE AGI   F   IWK ++ + + +          W++ +PSLPSAAY
Sbjct: 4   KSVFDASEIKSEFESAGINPKFAIQIWKYVIQNPDCV----------WDE-IPSLPSAAY 63

Query: 65  SLLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKPRPGG 124
           SLL SKFK LTS+++++  SSD  T+KLLIKLQ+GA VEAV+MRYD+RLG  G KPRPGG
Sbjct: 64  SLLHSKFKTLTSSLHSLFHSSDGTTSKLLIKLQNGAFVEAVVMRYDTRLGMLGGKPRPGG 123

Query: 125 LRSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLFQIRNVVFMGMGEP 184
           +RSTLCISSQVGCKMGC FCATGTMG+KSNLTSGEIVEQLVHASR+  IRN+VFMGMGEP
Sbjct: 124 IRSTLCISSQVGCKMGCTFCATGTMGFKSNLTSGEIVEQLVHASRIADIRNIVFMGMGEP 183

Query: 185 LNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAPAQDI 244
           LNNY+A+VEAVRVM+  PF LSPKRITISTVGI+HAI+KLH DLPG++LA+SLHAP Q+I
Sbjct: 184 LNNYNAVVEAVRVMLNQPFQLSPKRITISTVGIVHAINKLHNDLPGVSLAVSLHAPVQEI 243

Query: 245 RCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLETFLV 304
           RCQIMPAARAFPLQKLMDALQ +Q+ SQQKIFIEYIMLDGVND+EQHAH LG+LL+TF V
Sbjct: 244 RCQIMPAARAFPLQKLMDALQTFQKNSQQKIFIEYIMLDGVNDQEQHAHLLGELLKTFQV 303

Query: 305 VVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKL-IESPVNKQL 349
           V+NLIPFNPIGS+SQF+TSS + VS FQ++LR    I + + K++
Sbjct: 304 VINLIPFNPIGSTSQFETSSIQGVSRFQKILRETYKIRTTIRKEM 337

BLAST of Csor.00g000110 vs. TAIR 10
Match: AT2G39670.1 (Radical SAM superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 6.5e-43
Identity = 108/285 (37.89%), Postives = 165/285 (57.89%), Query Frame = 0

Query: 66  LLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKPRPGGL 125
           L+   FK   S +   V ++D  T KLL+KL+   L+E V        G        G  
Sbjct: 115 LVDGGFKVGRSPIYQTVTATD-GTIKLLLKLEDNLLIETV--------GIPVQDDEKGIT 174

Query: 126 RSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLF--QIRNVVFMGMGE 185
           R T C+SSQVGC + C FCATG  G+  NL   EI+EQ++    +F  ++ NVVFMGMGE
Sbjct: 175 RLTACVSSQVGCPLRCSFCATGKGGFSRNLQRHEIIEQVLAIEDVFKHRVTNVVFMGMGE 234

Query: 186 PLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAPAQD 245
           P+ N  ++++A R  +     +  + ITISTVG+ + I KL        LA+SLHAP Q 
Sbjct: 235 PMLNLKSVLDAHR-CLNKDIEIGQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQS 294

Query: 246 IRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLETF- 305
           +R +I+P+A+A+PL+ +M   ++Y Q++ +++  EY +L GVND+ +HA +L +LL  + 
Sbjct: 295 LREKIVPSAKAYPLEAIMKDCRDYFQETNRRVSFEYALLAGVNDQVEHAVELAELLREWG 354

Query: 306 -LVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKLIESPVNK 347
               VNLIP+NPI   S+++    + V AF   L ++ I + V +
Sbjct: 355 KTYHVNLIPYNPI-EGSEYQRPYKKAVLAFAAALESRKITASVRQ 388

BLAST of Csor.00g000110 vs. TAIR 10
Match: AT2G39670.2 (Radical SAM superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 6.5e-43
Identity = 108/285 (37.89%), Postives = 165/285 (57.89%), Query Frame = 0

Query: 66  LLRSKFKPLTSTVNTVVDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKPRPGGL 125
           L+   FK   S +   V ++D  T KLL+KL+   L+E V        G        G  
Sbjct: 118 LVDGGFKVGRSPIYQTVTATD-GTIKLLLKLEDNLLIETV--------GIPVQDDEKGIT 177

Query: 126 RSTLCISSQVGCKMGCRFCATGTMGYKSNLTSGEIVEQLVHASRLF--QIRNVVFMGMGE 185
           R T C+SSQVGC + C FCATG  G+  NL   EI+EQ++    +F  ++ NVVFMGMGE
Sbjct: 178 RLTACVSSQVGCPLRCSFCATGKGGFSRNLQRHEIIEQVLAIEDVFKHRVTNVVFMGMGE 237

Query: 186 PLNNYSALVEAVRVMIGSPFLLSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAPAQD 245
           P+ N  ++++A R  +     +  + ITISTVG+ + I KL        LA+SLHAP Q 
Sbjct: 238 PMLNLKSVLDAHR-CLNKDIEIGQRMITISTVGVPNTIKKLASHKLQSTLAVSLHAPNQS 297

Query: 246 IRCQIMPAARAFPLQKLMDALQEYQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLETF- 305
           +R +I+P+A+A+PL+ +M   ++Y Q++ +++  EY +L GVND+ +HA +L +LL  + 
Sbjct: 298 LREKIVPSAKAYPLEAIMKDCRDYFQETNRRVSFEYALLAGVNDQVEHAVELAELLREWG 357

Query: 306 -LVVVNLIPFNPIGSSSQFKTSSDEKVSAFQQVLRAKLIESPVNK 347
               VNLIP+NPI   S+++    + V AF   L ++ I + V +
Sbjct: 358 KTYHVNLIPYNPI-EGSEYQRPYKKAVLAFAAALESRKITASVRQ 391

BLAST of Csor.00g000110 vs. TAIR 10
Match: AT1G60230.1 (Radical SAM superfamily protein )

HSP 1 Score: 169.5 bits (428), Expect = 5.5e-42
Identity = 100/261 (38.31%), Postives = 150/261 (57.47%), Query Frame = 0

Query: 82  VDSSDQATTKLLIKLQSGALVEAVIMRYDSRLGKYGDKPRPGGLRSTLCISSQVGCKMGC 141
           + S+   T K+L  L  G ++E V++  D               R+T+C+SSQVGC M C
Sbjct: 164 IRSASDGTRKILFTLDDGLVIETVVIPCDRG-------------RTTVCVSSQVGCAMNC 223

Query: 142 RFCATGTMGYKSNLTSGEIVEQLVHASRLF-----QIRNVVFMGMGEPLNNYSALVEAVR 201
           +FC TG MG K NLT+ EIVEQ V+A RL       I NVVFMGMGEP +N   +++A  
Sbjct: 224 QFCYTGRMGLKRNLTTAEIVEQAVYARRLLSHEVGSITNVVFMGMGEPFHNIDNVIKAAN 283

Query: 202 VMIGSPFL-LSPKRITISTVGIIHAISKLHGDLPGLNLAISLHAPAQDIRCQIMPAARAF 261
           +M+    L  SP+++T+ST G++  + +   +     LA+SL+A   ++R  IMP  R +
Sbjct: 284 IMVDENGLHFSPRKVTVSTSGLVPQLKRFLRE-SNCALAVSLNATTDEVRNWIMPINRKY 343

Query: 262 PLQKLMDALQE-YQQKSQQKIFIEYIMLDGVNDEEQHAHQLGKLLETFLVVVNLIPFNPI 321
            L  L++ L+E    + + K+  EY+ML GVND    A +L +L++     +NLI FNP 
Sbjct: 344 KLSLLLETLREGLSSRHKYKVLFEYVMLAGVNDSMDDARRLVELVQGIPCKINLIQFNP- 403

Query: 322 GSSSQFKTSSDEKVSAFQQVL 336
            S SQF  + ++K+  F+ VL
Sbjct: 404 HSGSQFIQTEEDKMIKFRNVL 409

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0LQM13.5e-4941.48Dual-specificity RNA methyltransferase RlmN OS=Syntrophobacter fumaroxidans (str... [more]
Q1DCU15.9e-4941.70Dual-specificity RNA methyltransferase RlmN 1 OS=Myxococcus xanthus (strain DK16... [more]
A9A3L91.0e-4838.70Ribosomal RNA large subunit methyltransferase N OS=Nitrosopumilus maritimus (str... [more]
Q6MDD01.9e-4738.28Probable dual-specificity RNA methyltransferase RlmN 2 OS=Protochlamydia amoebop... [more]
Q1MQJ36.1e-4638.54Dual-specificity RNA methyltransferase RlmN OS=Lawsonia intracellularis (strain ... [more]
Match NameE-valueIdentityDescription
KAG6571920.17.43e-281100.00hypothetical protein SDJN03_28648, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023553823.15.94e-23496.28uncharacterized protein LOC111811282 [Cucurbita pepo subsp. pepo][more]
XP_022972434.11.20e-23395.99uncharacterized protein LOC111470995 [Cucurbita maxima][more]
XP_022953081.12.41e-23395.99uncharacterized protein LOC111455593 [Cucurbita moschata][more]
XP_038888929.11.11e-21588.25dual-specificity RNA methyltransferase RlmN [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1IBJ25.80e-23495.99uncharacterized protein LOC111470995 OS=Cucurbita maxima OX=3661 GN=LOC111470995... [more]
A0A6J1GM041.17e-23395.99uncharacterized protein LOC111455593 OS=Cucurbita moschata OX=3662 GN=LOC1114555... [more]
A0A0A0K4F51.23e-22486.89Radical_SAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G071380... [more]
A0A5D3C8Y42.96e-21386.53Dual-specificity RNA methyltransferase RlmN OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3C1672.96e-21386.53dual-specificity RNA methyltransferase RlmN OS=Cucumis melo OX=3656 GN=LOC103495... [more]
Match NameE-valueIdentityDescription
AT3G19630.18.0e-13469.57Radical SAM superfamily protein [more]
AT2G39670.16.5e-4337.89Radical SAM superfamily protein [more]
AT2G39670.26.5e-4337.89Radical SAM superfamily protein [more]
AT1G60230.15.5e-4238.31Radical SAM superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF13394Fer4_14coord: 136..221
e-value: 4.4E-6
score: 26.8
NoneNo IPR availablePANTHERPTHR30544:SF8RADICAL SAM SUPERFAMILY PROTEINcoord: 3..337
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 136..336
e-value: 9.74725E-8
score: 50.0246
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 127..316
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 132..290
e-value: 5.0E-14
score: 53.0
IPR007197Radical SAMSFLDSFLDS00029Radical_SAMcoord: 132..290
e-value: 7.5E-12
score: 40.5
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 123..351
score: 29.255884
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 76..348
e-value: 3.3E-95
score: 320.5
IPR040072Methyltransferase (Class A)PANTHERPTHR3054423S RRNA METHYLTRANSFERASEcoord: 3..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g000110.m01Csor.00g000110.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070475 rRNA base methylation
biological_process GO:0030488 tRNA methylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008173 RNA methyltransferase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding