Tan0022795 (gene) Snake gourd v1

Overview
NameTan0022795
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionOmega-3 fatty acid desaturase
LocationLG04: 13611670 .. 13614078 (-)
RNA-Seq ExpressionTan0022795
SyntenyTan0022795
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAGGTGGGTCTTATCTGAATGTGGTGTTAAGCCCCTCCCAAGGATCTACCCTCAGCCTTCTAGGAATGGACTTGCTTCTAGGACTTGTATGGTTTCTGAGATTAGGTCGCCATTGTTGGCCTCTAAAGCTCACTTTCTCCGTTCTTCTTTGAGTCTTTCCACTAAGGGGAAGAGTTGGGCTCTGAATGTCAGTGCTCCTGTGAGAGTTGCTTCTGTTGATGAGGATAATGAGAGGGAGAGAGTTGATGGGGTTAATGGGGTTGAGGATGAGGGTGAGGGTGAATTCGACCCGGCTGCTGCTCCGCCATTTCGGTTGGCCGATATTCGAGCCGCCATCCCGAAGCATTGCTGGGTTAAGGATCCTTGGAGGTCGATGAGCTATGTTGTGAGGGATGTGATTGTGGTGTTTGGATTGGCGGCTGTTGCTGCTTACTTCAACAACTGGGTTGTTTGGCCTCTTTACTGGTTTGCTCAAGGGACCATGTTTTGGGCTCTTTTTGTTCTTGGTCATGATTGGTAATTTTCTCTCTGTTGAAGCCATTGTTAATCTTTACTTTTGGTCTTAGAATTCCATTGCCTGAATGTTATGTGTTTTCCTAATGGTTGGTTTGATTTCATTGGTGAATGTGCAGTGGCCATGGAAGTTTCTCTAATGACCCAAAACTGAATAGTGTTGTTGGCCATATTCTTCATTCTTCAATTCTAGTTCCATATCATGGATGGTTAGTTTCTGATTATCTTTCTGATTATGTTTGGTTAATTATTCTTTTCTGTTGCTTTAACTCTGTGTTTTGTATGTTAAGTGGTTTTATTTTATCTTGTTGCTTAACTCAGGAGAATAAGCCACAGAACTCATCACCAGAACCACGGCCATGTCGAAAACGATGAATCGTGGCATCCTGTAAGTTCTTTGTAGCTAGCCTTTCTAAATTCTTTATCCCAAGGAAGCAGTTTGTTACAAGAAATGGTCATTTTGTTGTGTCACGTTCAATAGCAATATGTTCGTATATGTTTTGTAGTTGTCCGAGAAAATCTATAGGAGTTTGGATAAAGCAACCAGAACATTGAGGTTCACCTTGCCTTTCCCCATGCTTGCATACCCTGTCTATCTGGTTAGTACGCTCTGTTTCTTTATCCTTCTTTATGAACTTCAATGTTATGCGAATGTTTTGATGGATTCTAATGCATCTTGTTGGTGATATTACAGTGGAGTAGAAGTCCTGGAAAGAAGGGTTCTCATTTTGACCCGAATAGTGATTTGTTCGTCCCGAGCGAGCGGAATGATATCATTACATCCACAGCGTGTTGGACTGCAATGCTTGCTCTGCTTGGGTGTCTATCTGTAGTGATGGGTCCCCTCCAAATCCTCAAACTTTATGGCATTCCCTACCTGGTGAGTTCAGCTTCGCTCACAAATTTGTGATGAATTCTTTCCAGCTTTCACTCAACTTCTACATTCAGGCGACTTTTTTTTTTTCTTTTTTTCGATACAATCATTCACTTGTGCACTCCTCTGAATGTTTTTCAAATGTAGATGTTTGTTATGTGGCTGGACGTGGTCACATACCTGCATCACCATGGTCACGGCGATAAACTTCCTTGGTATCGTGGAGAGGTACGCTGATCAATCTTTTCAAACAACAACGATACATCACAAAGTGGTATGTTTGACACCATTTTATTAACTTATCTGGCTCTGCATTTCATGATCAAACAGGAATGGAGTTACTTGAGAGGAGGATTAACCACTCTCGATCGCGATTATGGATGGATCAACAACATCCACCATGACATTGGAACCCATGTCATTCACCATCTCTTCCCTCAGATCCCACACTATCACCTAATAGAAGCAGTGAGTATCTCTCTCACCTTTGTATCTGAAATCCAAACTTAACTTTGTTCTATATTTTCAAAATTCTCATCGCAACTGCAATCTTATCATGTTAGACTGAGGCAACAAAGCCAGTGCTCGGGAAGTATTATCGAGAACCGGAGAAATCTTGGCCTCTCCCATTTCACTTATTGGGAGTTCTTGTAAAAAGCATGAAAAAGGATCACTTTGTGAGCGATTCAGGGGACATTGTATATTATCAGACCGACCCCGACCTTTCTTGATCAAAGCAACAGGACTGAGATACTGCTCATTCTCTTAACTAGTATATCAAAATTCTTTTCGAATAGCTTGCGAGCCATTCGAGTTTGAAATATGATCACGCAGTTGCCAAAAATAAAACTTCAATAGAGAAGAAGAAGCTGCTTGTTCATGGTGAAAGTATTAATGCCTTAAGGGTATTAAGAGTTGCATATTATTATTGCCATATGAAAGAGAAATATGACATTATTTTTGCTTGAAATCTGTTGAATAACTATTAGTGAAAATATTTGGATGCTTTAACATCAA

mRNA sequence

ATGGCGAGGTGGGTCTTATCTGAATGTGGTGTTAAGCCCCTCCCAAGGATCTACCCTCAGCCTTCTAGGAATGGACTTGCTTCTAGGACTTGTATGGTTTCTGAGATTAGGTCGCCATTGTTGGCCTCTAAAGCTCACTTTCTCCGTTCTTCTTTGAGTCTTTCCACTAAGGGGAAGAGTTGGGCTCTGAATGTCAGTGCTCCTGTGAGAGTTGCTTCTGTTGATGAGGATAATGAGAGGGAGAGAGTTGATGGGGTTAATGGGGTTGAGGATGAGGGTGAGGGTGAATTCGACCCGGCTGCTGCTCCGCCATTTCGGTTGGCCGATATTCGAGCCGCCATCCCGAAGCATTGCTGGGTTAAGGATCCTTGGAGGTCGATGAGCTATGTTGTGAGGGATGTGATTGTGGTGTTTGGATTGGCGGCTGTTGCTGCTTACTTCAACAACTGGGTTGTTTGGCCTCTTTACTGGTTTGCTCAAGGGACCATGTTTTGGGCTCTTTTTGTTCTTGGTCATGATTGTGGCCATGGAAGTTTCTCTAATGACCCAAAACTGAATAGTGTTGTTGGCCATATTCTTCATTCTTCAATTCTAGTTCCATATCATGGATGGAGAATAAGCCACAGAACTCATCACCAGAACCACGGCCATGTCGAAAACGATGAATCGTGGCATCCTTTGTCCGAGAAAATCTATAGGAGTTTGGATAAAGCAACCAGAACATTGAGGTTCACCTTGCCTTTCCCCATGCTTGCATACCCTGTCTATCTGTGGAGTAGAAGTCCTGGAAAGAAGGGTTCTCATTTTGACCCGAATAGTGATTTGTTCGTCCCGAGCGAGCGGAATGATATCATTACATCCACAGCGTGTTGGACTGCAATGCTTGCTCTGCTTGGGTGTCTATCTGTAGTGATGGGTCCCCTCCAAATCCTCAAACTTTATGGCATTCCCTACCTGGTGAGTTCAGCTTCGCTCACAAATTTGTGATGAATTCTTTCCAGCTTTCACTCAACTTCTACATTCAGGCGACTTTTTTTTTTTCTTTTTTTCGATACAATCATTCACTTGTGCACTCCTCTGAATGTTTTTCAAATGTAGATGTTTGTTATGTGGCTGGACGTGGTCACATACCTGCATCACCATGGTCACGGCGATAAACTTCCTTGGTATCGTGGAGAGGTACGCTGATCAATCTTTTCAAACAACAACGATACATCACAAAGTGGTATGTTTGACACCATTTTATTAACTTATCTGGCTCTGCATTTCATGATCAAACAGGAATGGAGTTACTTGAGAGGAGGATTAACCACTCTCGATCGCGATTATGGATGGATCAACAACATCCACCATGACATTGGAACCCATGTCATTCACCATCTCTTCCCTCAGATCCCACACTATCACCTAATAGAAGCAGTGAGTATCTCTCTCACCTTTGTATCTGAAATCCAAACTTAACTTTGTTCTATATTTTCAAAATTCTCATCGCAACTGCAATCTTATCATGTTAGACTGAGGCAACAAAGCCAGTGCTCGGGAAGTATTATCGAGAACCGGAGAAATCTTGGCCTCTCCCATTTCACTTATTGGGAGTTCTTGTAAAAAGCATGAAAAAGGATCACTTTGTGAGCGATTCAGGGGACATTGTATATTATCAGACCGACCCCGACCTTTCTTGATCAAAGCAACAGGACTGAGATACTGCTCATTCTCTTAACTAGTATATCAAAATTCTTTTCGAATAGCTTGCGAGCCATTCGAGTTTGAAATATGATCACGCAGTTGCCAAAAATAAAACTTCAATAGAGAAGAAGAAGCTGCTTGTTCATGGTGAAAGTATTAATGCCTTAAGGGTATTAAGAGTTGCATATTATTATTGCCATATGAAAGAGAAATATGACATTATTTTTGCTTGAAATCTGTTGAATAACTATTAGTGAAAATATTTGGATGCTTTAACATCAA

Coding sequence (CDS)

ATGGCGAGGTGGGTCTTATCTGAATGTGGTGTTAAGCCCCTCCCAAGGATCTACCCTCAGCCTTCTAGGAATGGACTTGCTTCTAGGACTTGTATGGTTTCTGAGATTAGGTCGCCATTGTTGGCCTCTAAAGCTCACTTTCTCCGTTCTTCTTTGAGTCTTTCCACTAAGGGGAAGAGTTGGGCTCTGAATGTCAGTGCTCCTGTGAGAGTTGCTTCTGTTGATGAGGATAATGAGAGGGAGAGAGTTGATGGGGTTAATGGGGTTGAGGATGAGGGTGAGGGTGAATTCGACCCGGCTGCTGCTCCGCCATTTCGGTTGGCCGATATTCGAGCCGCCATCCCGAAGCATTGCTGGGTTAAGGATCCTTGGAGGTCGATGAGCTATGTTGTGAGGGATGTGATTGTGGTGTTTGGATTGGCGGCTGTTGCTGCTTACTTCAACAACTGGGTTGTTTGGCCTCTTTACTGGTTTGCTCAAGGGACCATGTTTTGGGCTCTTTTTGTTCTTGGTCATGATTGTGGCCATGGAAGTTTCTCTAATGACCCAAAACTGAATAGTGTTGTTGGCCATATTCTTCATTCTTCAATTCTAGTTCCATATCATGGATGGAGAATAAGCCACAGAACTCATCACCAGAACCACGGCCATGTCGAAAACGATGAATCGTGGCATCCTTTGTCCGAGAAAATCTATAGGAGTTTGGATAAAGCAACCAGAACATTGAGGTTCACCTTGCCTTTCCCCATGCTTGCATACCCTGTCTATCTGTGGAGTAGAAGTCCTGGAAAGAAGGGTTCTCATTTTGACCCGAATAGTGATTTGTTCGTCCCGAGCGAGCGGAATGATATCATTACATCCACAGCGTGTTGGACTGCAATGCTTGCTCTGCTTGGGTGTCTATCTGTAGTGATGGGTCCCCTCCAAATCCTCAAACTTTATGGCATTCCCTACCTGGTGAGTTCAGCTTCGCTCACAAATTTGTGA

Protein sequence

MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKSWALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWVKDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATRTLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLSVVMGPLQILKLYGIPYLVSSASLTNL
Homology
BLAST of Tan0022795 vs. ExPASy Swiss-Prot
Match: P48619 (Omega-3 fatty acid desaturase, chloroplastic OS=Ricinus communis OX=3988 GN=FAD7A-1 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 9.0e-136
Identity = 239/326 (73.31%), Postives = 273/326 (83.74%), Query Frame = 0

Query: 2   ARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLS----TK 61
           A WVLSECG++PLPRIY +P R G  S+T  + ++R  L  SK++ L SS  +S    +K
Sbjct: 3   AGWVLSECGLRPLPRIYSRP-RIGFTSKTTNLLKLRE-LPDSKSYNLCSSFKVSSWSNSK 62

Query: 62  GKSWALNVSAPVRVASV--DEDNERERVDGVNGVEDEGEGE-FDPAAAPPFRLADIRAAI 121
             +WALNV+ PV V++V  ++D ERE  +G+  V DEG+GE FD  A PPF LADIRAAI
Sbjct: 63  QSNWALNVAVPVNVSTVSGEDDREREEFNGIVNV-DEGKGEFFDAGAPPPFTLADIRAAI 122

Query: 122 PKHCWVKDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDC 181
           PKHCWVK+PWRSMSYV+RDV+VVFGLAAVAAYFNNWV WPLYWF QGTMFWALFVLGHDC
Sbjct: 123 PKHCWVKNPWRSMSYVLRDVVVVFGLAAVAAYFNNWVAWPLYWFCQGTMFWALFVLGHDC 182

Query: 182 GHGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRS 241
           GHGSFSN+PKLNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKI++S
Sbjct: 183 GHGSFSNNPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFKS 242

Query: 242 LDKATRTLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAM 301
           LD  T+TLRF+LPFPMLAYP YLWSRSPGKKGSHF P+S LFVP ER DIITSTACWTAM
Sbjct: 243 LDNVTKTLRFSLPFPMLAYPFYLWSRSPGKKGSHFHPDSGLFVPKERKDIITSTACWTAM 302

Query: 302 LALLGCLSVVMGPLQILKLYGIPYLV 321
            ALL  L+  MGP+Q+LKLYGIPY +
Sbjct: 303 AALLVYLNFSMGPVQMLKLYGIPYWI 325

BLAST of Tan0022795 vs. ExPASy Swiss-Prot
Match: P48620 (Omega-3 fatty acid desaturase, chloroplastic OS=Sesamum indicum OX=4182 GN=FAD7 PE=2 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 2.6e-135
Identity = 238/325 (73.23%), Postives = 267/325 (82.15%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNG---LASRTCMVSEIRSPLLASKAHFLRSSLSLSTK 60
           MA WVLSECG++PLPR+YP+P R G   L S    +   R+ L    +  L S +    +
Sbjct: 1   MASWVLSECGLRPLPRVYPKP-RTGHPLLNSNPTKLRFSRTDLGNGSSFCLSSGI---LR 60

Query: 61  GKSWALNVSAPVRVASVDEDNER---ERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAI 120
            K+WAL VSAP+RV  V+E+ E    ERV  +NG E     EFDP A PPF+L+DIR AI
Sbjct: 61  EKNWALRVSAPLRVLQVEEEEENKEGERV--INGGE-----EFDPGAPPPFKLSDIREAI 120

Query: 121 PKHCWVKDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDC 180
           PKHCWVKDPWRSM YVVRDV VVFGLAAVAAYFNNWVVWPLYWFAQ TMFWALFVLGHDC
Sbjct: 121 PKHCWVKDPWRSMGYVVRDVAVVFGLAAVAAYFNNWVVWPLYWFAQSTMFWALFVLGHDC 180

Query: 181 GHGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRS 240
           GHGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIY++
Sbjct: 181 GHGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYKN 240

Query: 241 LDKATRTLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAM 300
           LD AT+ LRFTLPFP+LAYP+YLWSRSPGK+GSHF P+SDLFVP+E+ D+ITST CWTAM
Sbjct: 241 LDTATKKLRFTLPFPLLAYPIYLWSRSPGKQGSHFHPDSDLFVPNEKKDVITSTVCWTAM 300

Query: 301 LALLGCLSVVMGPLQILKLYGIPYL 320
           LALL  LS V+GP+Q+LKLYGIPYL
Sbjct: 301 LALLVGLSFVIGPVQLLKLYGIPYL 314

BLAST of Tan0022795 vs. ExPASy Swiss-Prot
Match: P48621 (Omega-3 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 GN=FAD7 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 2.4e-128
Identity = 218/321 (67.91%), Postives = 254/321 (79.13%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MA W   +CG+KPL  + P+P      S T  V  + +  + +   F    L  + + ++
Sbjct: 1   MATWYHQKCGLKPLAPVIPRPRTGAALSSTSRVEFLDTNKVVAGPKF--QPLRCNLRERN 60

Query: 61  WALNVSAPVRVASVDEDNER-ERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCW 120
           W L VSAP+RVAS++E+ +  +  +G NGVE E   EFDP A PPF LADIRAAIPKHCW
Sbjct: 61  WGLKVSAPLRVASIEEEQKSVDLTNGTNGVEHEKLPEFDPGAPPPFNLADIRAAIPKHCW 120

Query: 121 VKDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSF 180
           VKDPWRSMSYVVRDVI VFGLAA AAY NNW+VWPLYW AQGTMFWALFVLGHDCGHGSF
Sbjct: 121 VKDPWRSMSYVVRDVIAVFGLAAAAAYLNNWLVWPLYWAAQGTMFWALFVLGHDCGHGSF 180

Query: 181 SNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKAT 240
           SN+ KLNSVVGH+LHSSILVPYHGWRISHRTHHQ+HGH ENDESWHPL EK++RSLD  T
Sbjct: 181 SNNSKLNSVVGHLLHSSILVPYHGWRISHRTHHQHHGHAENDESWHPLPEKLFRSLDTVT 240

Query: 241 RTLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLG 300
           R LRFT PFP+LA+PVYL+SRSPGK GSHFDP+SDLFVP+ER D+ITSTACW AML LL 
Sbjct: 241 RMLRFTAPFPLLAFPVYLFSRSPGKTGSHFDPSSDLFVPNERKDVITSTACWAAMLGLLV 300

Query: 301 CLSVVMGPLQILKLYGIPYLV 321
            L  VMGP+Q+LKLYG+PY++
Sbjct: 301 GLGFVMGPIQLLKLYGVPYVI 319

BLAST of Tan0022795 vs. ExPASy Swiss-Prot
Match: P48622 (Temperature-sensitive sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD8 PE=1 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 2.5e-125
Identity = 212/320 (66.25%), Postives = 246/320 (76.88%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MA  VLSECG +PLPR YP+ +    AS      +   P L   +  L S     +K ++
Sbjct: 1   MASSVLSECGFRPLPRFYPKHT-TSFASNPKPTFKFNPP-LKPPSSLLNSRYGFYSKTRN 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNV+ P+       + + ER              FDP A PPF LADIRAAIPKHCWV
Sbjct: 61  WALNVATPLTTLQSPSEEDTER--------------FDPGAPPPFNLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           K+PW SMSYVVRDV +VFGLAAVAAYFNNW++WPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 KNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDP+LNSV GH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL E IY++L+K T+
Sbjct: 181 NDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQ 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
             RFTLPFPMLAYP YLW+RSPGK+GSH+ P+SDLF+P E+ D++TSTACWTAM ALL C
Sbjct: 241 MFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           L+ VMGP+Q+LKLYGIPY +
Sbjct: 301 LNFVMGPIQMLKLYGIPYWI 304

BLAST of Tan0022795 vs. ExPASy Swiss-Prot
Match: P46310 (sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD7 PE=1 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 7.2e-125
Identity = 214/326 (65.64%), Postives = 250/326 (76.69%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKG-- 60
           MA  VLSECG++PLPRIY  P  N L++     ++ R  L +S      S LS       
Sbjct: 1   MANLVLSECGIRPLPRIYTTPRSNFLSNN----NKFRPSLSSSSYKTSSSPLSFGLNSRD 60

Query: 61  ---KSWALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIP 120
              ++WALNVS P+     +E          + +E++ +  FDP A PPF LADIRAAIP
Sbjct: 61  GFTRNWALNVSTPLTTPIFEE----------SPLEEDNKQRFDPGAPPPFNLADIRAAIP 120

Query: 121 KHCWVKDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCG 180
           KHCWVK+PW+S+SYVVRDV +VF LAA AAY NNW+VWPLYW AQGTMFWALFVLGHDCG
Sbjct: 121 KHCWVKNPWKSLSYVVRDVAIVFALAAGAAYLNNWIVWPLYWLAQGTMFWALFVLGHDCG 180

Query: 181 HGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSL 240
           HGSFSNDPKLNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHP+SEKIY +L
Sbjct: 181 HGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPMSEKIYNTL 240

Query: 241 DKATRTLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAML 300
           DK TR  RFTLP  MLAYP YLW+RSPGKKGSH+ P+SDLF+P ER D++TSTACWTAM 
Sbjct: 241 DKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLFLPKERKDVLTSTACWTAMA 300

Query: 301 ALLGCLSVVMGPLQILKLYGIPYLVS 322
           ALL CL+  +GP+Q+LKLYGIPY ++
Sbjct: 301 ALLVCLNFTIGPIQMLKLYGIPYWIN 312

BLAST of Tan0022795 vs. NCBI nr
Match: XP_038892287.1 (omega-3 fatty acid desaturase, chloroplastic [Benincasa hispida])

HSP 1 Score: 637.9 bits (1644), Expect = 4.9e-179
Identity = 304/320 (95.00%), Postives = 314/320 (98.12%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MARWVLSECGVKPLPRIYPQPSRNG+AS+T MVSEIR PLLASKAH LRSSL LST+GKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGIASKTFMVSEIRQPLLASKAHVLRSSLGLSTRGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNVSAPVRVAS+DEDNE+ERVDG+NGVED  EG+FDPAAAPPFRLADIRAAIPKHCWV
Sbjct: 61  WALNVSAPVRVASIDEDNEKERVDGINGVED--EGDFDPAAAPPFRLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           KDPW+SMSYVVRDV+VVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 KDPWKSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR
Sbjct: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
           TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC
Sbjct: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           LSVVMGPLQILKLYGIPYL+
Sbjct: 301 LSVVMGPLQILKLYGIPYLM 318

BLAST of Tan0022795 vs. NCBI nr
Match: XP_008465212.1 (PREDICTED: omega-3 fatty acid desaturase, chloroplastic [Cucumis melo] >KAA0052881.1 omega-3 fatty acid desaturase [Cucumis melo var. makuwa] >TYK14479.1 omega-3 fatty acid desaturase [Cucumis melo var. makuwa])

HSP 1 Score: 623.6 bits (1607), Expect = 9.5e-175
Identity = 299/320 (93.44%), Postives = 306/320 (95.62%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MARWVLSECGVKPLPRIYPQPSRNG AS+T MVSEIR PLL SKAH LRSSL +STKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGFASKTFMVSEIRQPLLTSKAHVLRSSLGISTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNVS PV +ASVDEDNERERVD VNGVED  EG FDPAA PPFRLADIRAAIPKHCWV
Sbjct: 61  WALNVSTPVVLASVDEDNERERVDEVNGVED--EGGFDPAAPPPFRLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           KDPWRSMSYVVRDV+VVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 KDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDP LNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEK+YRSLDKATR
Sbjct: 181 NDPNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKVYRSLDKATR 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
           TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC
Sbjct: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           LSVVMGPLQILKLYGIPYL+
Sbjct: 301 LSVVMGPLQILKLYGIPYLM 318

BLAST of Tan0022795 vs. NCBI nr
Match: NP_001274404.1 (omega-3 fatty acid desaturase [Cucumis sativus] >ACE80931.1 omega-3 fatty acid desaturase [Cucumis sativus] >KGN50667.1 hypothetical protein Csa_005845 [Cucumis sativus])

HSP 1 Score: 620.5 bits (1599), Expect = 8.0e-174
Identity = 298/320 (93.12%), Postives = 305/320 (95.31%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MARWVLSECGVKPLPRIYP PSRNG AS+T MVSEIR PLLASKAH LRSSL LSTKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPLPSRNGFASKTFMVSEIRQPLLASKAHVLRSSLGLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNVS PV VASVDEDNERERV+ VNGVED  EG FDPAA PPFRLADIRAAIPKHCWV
Sbjct: 61  WALNVSTPVAVASVDEDNERERVNEVNGVED--EGSFDPAAPPPFRLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           +DPWRSMSYVVRDV+VVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 RDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDP LNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR
Sbjct: 181 NDPNLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
           TLRF LPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC
Sbjct: 241 TLRFALPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           LSVVMGPLQI+KLYGIPY++
Sbjct: 301 LSVVMGPLQIIKLYGIPYMM 318

BLAST of Tan0022795 vs. NCBI nr
Match: KAG7030666.1 (Omega-3 fatty acid desaturase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 616.3 bits (1588), Expect = 1.5e-172
Identity = 294/320 (91.88%), Postives = 306/320 (95.62%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MARWVLSECGVK LPRIYP+PSRNGLASRTCMVSEIRS  + SK HFLRSSLSLSTKGKS
Sbjct: 1   MARWVLSECGVKSLPRIYPRPSRNGLASRTCMVSEIRSSSVPSKPHFLRSSLSLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNVSAPVRVASVDEDNE+++VDGV     E EG FDPAAAPPFRLADIRAAIPKHCWV
Sbjct: 61  WALNVSAPVRVASVDEDNEKDKVDGV-----ENEGGFDPAAAPPFRLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           KDPWRSMSYVVRDV+VVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 KDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIY++LDKAT+
Sbjct: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYKTLDKATK 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
           TLRFTLPFPMLAYP+YLWSRSPGKKGSHFDPNSDLFVPSERNDIITST CWTAMLALLGC
Sbjct: 241 TLRFTLPFPMLAYPIYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           LS VMGPLQILKLYGIPY++
Sbjct: 301 LSAVMGPLQILKLYGIPYVM 315

BLAST of Tan0022795 vs. NCBI nr
Match: XP_022942829.1 (omega-3 fatty acid desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 616.3 bits (1588), Expect = 1.5e-172
Identity = 294/320 (91.88%), Postives = 306/320 (95.62%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MARWVLSECGVK LPRIYP+PSRNGLASRTCMVSEIRS  + SK HFLRSSLSLSTKGKS
Sbjct: 1   MARWVLSECGVKSLPRIYPRPSRNGLASRTCMVSEIRSSSVPSKPHFLRSSLSLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNVSAPVRVASVDEDNE+++VDGV     E EG FDPAAAPPFRLADIRAAIPKHCWV
Sbjct: 61  WALNVSAPVRVASVDEDNEKDKVDGV-----ENEGGFDPAAAPPFRLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           KDPWRSMSYVVRDV+VVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 KDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIY++LDKAT+
Sbjct: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYKTLDKATK 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
           TLRFTLPFPMLAYP+YLWSRSPGKKGSHFDPNSDLFVPSERNDIITST CWTAMLALLGC
Sbjct: 241 TLRFTLPFPMLAYPIYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           LS VMGPLQILKLYGIPY++
Sbjct: 301 LSAVMGPLQILKLYGIPYVM 315

BLAST of Tan0022795 vs. ExPASy TrEMBL
Match: A0A5A7UH66 (Omega-3 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold186G001460 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 4.6e-175
Identity = 299/320 (93.44%), Postives = 306/320 (95.62%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MARWVLSECGVKPLPRIYPQPSRNG AS+T MVSEIR PLL SKAH LRSSL +STKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGFASKTFMVSEIRQPLLTSKAHVLRSSLGISTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNVS PV +ASVDEDNERERVD VNGVED  EG FDPAA PPFRLADIRAAIPKHCWV
Sbjct: 61  WALNVSTPVVLASVDEDNERERVDEVNGVED--EGGFDPAAPPPFRLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           KDPWRSMSYVVRDV+VVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 KDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDP LNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEK+YRSLDKATR
Sbjct: 181 NDPNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKVYRSLDKATR 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
           TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC
Sbjct: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           LSVVMGPLQILKLYGIPYL+
Sbjct: 301 LSVVMGPLQILKLYGIPYLM 318

BLAST of Tan0022795 vs. ExPASy TrEMBL
Match: A0A1S3CNS0 (omega-3 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502871 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 4.6e-175
Identity = 299/320 (93.44%), Postives = 306/320 (95.62%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MARWVLSECGVKPLPRIYPQPSRNG AS+T MVSEIR PLL SKAH LRSSL +STKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPQPSRNGFASKTFMVSEIRQPLLTSKAHVLRSSLGISTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNVS PV +ASVDEDNERERVD VNGVED  EG FDPAA PPFRLADIRAAIPKHCWV
Sbjct: 61  WALNVSTPVVLASVDEDNERERVDEVNGVED--EGGFDPAAPPPFRLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           KDPWRSMSYVVRDV+VVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 KDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDP LNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEK+YRSLDKATR
Sbjct: 181 NDPNLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKVYRSLDKATR 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
           TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC
Sbjct: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           LSVVMGPLQILKLYGIPYL+
Sbjct: 301 LSVVMGPLQILKLYGIPYLM 318

BLAST of Tan0022795 vs. ExPASy TrEMBL
Match: B3VA19 (Omega-3 fatty acid desaturase OS=Cucumis sativus OX=3659 GN=fad7 PE=2 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 3.9e-174
Identity = 298/320 (93.12%), Postives = 305/320 (95.31%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MARWVLSECGVKPLPRIYP PSRNG AS+T MVSEIR PLLASKAH LRSSL LSTKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPLPSRNGFASKTFMVSEIRQPLLASKAHVLRSSLGLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNVS PV VASVDEDNERERV+ VNGVED  EG FDPAA PPFRLADIRAAIPKHCWV
Sbjct: 61  WALNVSTPVAVASVDEDNERERVNEVNGVED--EGSFDPAAPPPFRLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           +DPWRSMSYVVRDV+VVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 RDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDP LNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR
Sbjct: 181 NDPNLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
           TLRF LPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC
Sbjct: 241 TLRFALPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           LSVVMGPLQI+KLYGIPY++
Sbjct: 301 LSVVMGPLQIIKLYGIPYMM 318

BLAST of Tan0022795 vs. ExPASy TrEMBL
Match: A0A6J1FSG4 (omega-3 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111447744 PE=3 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 7.3e-173
Identity = 294/320 (91.88%), Postives = 306/320 (95.62%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MARWVLSECGVK LPRIYP+PSRNGLASRTCMVSEIRS  + SK HFLRSSLSLSTKGKS
Sbjct: 1   MARWVLSECGVKSLPRIYPRPSRNGLASRTCMVSEIRSSSVPSKPHFLRSSLSLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNVSAPVRVASVDEDNE+++VDGV     E EG FDPAAAPPFRLADIRAAIPKHCWV
Sbjct: 61  WALNVSAPVRVASVDEDNEKDKVDGV-----ENEGGFDPAAAPPFRLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           KDPWRSMSYVVRDV+VVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 KDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIY++LDKAT+
Sbjct: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYKTLDKATK 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
           TLRFTLPFPMLAYP+YLWSRSPGKKGSHFDPNSDLFVPSERNDIITST CWTAMLALLGC
Sbjct: 241 TLRFTLPFPMLAYPIYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           LS VMGPLQILKLYGIPY++
Sbjct: 301 LSAVMGPLQILKLYGIPYVM 315

BLAST of Tan0022795 vs. ExPASy TrEMBL
Match: A0A6J1K2M7 (omega-3 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491157 PE=3 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.4e-171
Identity = 294/320 (91.88%), Postives = 305/320 (95.31%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MARWVLSECGVKPLPRIYP+PSRNGLASRT MVSEIR   + SK +FLRSSLSLSTKGKS
Sbjct: 1   MARWVLSECGVKPLPRIYPRPSRNGLASRTYMVSEIRFSSVPSKPNFLRSSLSLSTKGKS 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNVSAP RVASVDEDNE+++VDGV     E EG FDPAAAPPFRLADIRAAIPKHCWV
Sbjct: 61  WALNVSAPARVASVDEDNEKDKVDGV-----ENEGGFDPAAAPPFRLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           KDPWRSMSYVVRDV+VVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 KDPWRSMSYVVRDVVVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKI+R+LDKATR
Sbjct: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIFRTLDKATR 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
           TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITST CWTAMLALLGC
Sbjct: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTVCWTAMLALLGC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           LSVVMGPLQILKLYGIPY++
Sbjct: 301 LSVVMGPLQILKLYGIPYVM 315

BLAST of Tan0022795 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 449.9 bits (1156), Expect = 1.8e-126
Identity = 212/320 (66.25%), Postives = 246/320 (76.88%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MA  VLSECG +PLPR YP+ +    AS      +   P L   +  L S     +K ++
Sbjct: 1   MASSVLSECGFRPLPRFYPKHT-TSFASNPKPTFKFNPP-LKPPSSLLNSRYGFYSKTRN 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNV+ P+       + + ER              FDP A PPF LADIRAAIPKHCWV
Sbjct: 61  WALNVATPLTTLQSPSEEDTER--------------FDPGAPPPFNLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           K+PW SMSYVVRDV +VFGLAAVAAYFNNW++WPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 KNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDP+LNSV GH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL E IY++L+K T+
Sbjct: 181 NDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQ 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
             RFTLPFPMLAYP YLW+RSPGK+GSH+ P+SDLF+P E+ D++TSTACWTAM ALL C
Sbjct: 241 MFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           L+ VMGP+Q+LKLYGIPY +
Sbjct: 301 LNFVMGPIQMLKLYGIPYWI 304

BLAST of Tan0022795 vs. TAIR 10
Match: AT5G05580.2 (fatty acid desaturase 8 )

HSP 1 Score: 449.9 bits (1156), Expect = 1.8e-126
Identity = 212/320 (66.25%), Postives = 246/320 (76.88%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKGKS 60
           MA  VLSECG +PLPR YP+ +    AS      +   P L   +  L S     +K ++
Sbjct: 1   MASSVLSECGFRPLPRFYPKHT-TSFASNPKPTFKFNPP-LKPPSSLLNSRYGFYSKTRN 60

Query: 61  WALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIPKHCWV 120
           WALNV+ P+       + + ER              FDP A PPF LADIRAAIPKHCWV
Sbjct: 61  WALNVATPLTTLQSPSEEDTER--------------FDPGAPPPFNLADIRAAIPKHCWV 120

Query: 121 KDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFS 180
           K+PW SMSYVVRDV +VFGLAAVAAYFNNW++WPLYWFAQGTMFWALFVLGHDCGHGSFS
Sbjct: 121 KNPWMSMSYVVRDVAIVFGLAAVAAYFNNWLLWPLYWFAQGTMFWALFVLGHDCGHGSFS 180

Query: 181 NDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATR 240
           NDP+LNSV GH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHPL E IY++L+K T+
Sbjct: 181 NDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQ 240

Query: 241 TLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGC 300
             RFTLPFPMLAYP YLW+RSPGK+GSH+ P+SDLF+P E+ D++TSTACWTAM ALL C
Sbjct: 241 MFRFTLPFPMLAYPFYLWNRSPGKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVC 300

Query: 301 LSVVMGPLQILKLYGIPYLV 321
           L+ VMGP+Q+LKLYGIPY +
Sbjct: 301 LNFVMGPIQMLKLYGIPYWI 304

BLAST of Tan0022795 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 448.4 bits (1152), Expect = 5.1e-126
Identity = 214/326 (65.64%), Postives = 250/326 (76.69%), Query Frame = 0

Query: 1   MARWVLSECGVKPLPRIYPQPSRNGLASRTCMVSEIRSPLLASKAHFLRSSLSLSTKG-- 60
           MA  VLSECG++PLPRIY  P  N L++     ++ R  L +S      S LS       
Sbjct: 1   MANLVLSECGIRPLPRIYTTPRSNFLSNN----NKFRPSLSSSSYKTSSSPLSFGLNSRD 60

Query: 61  ---KSWALNVSAPVRVASVDEDNERERVDGVNGVEDEGEGEFDPAAAPPFRLADIRAAIP 120
              ++WALNVS P+     +E          + +E++ +  FDP A PPF LADIRAAIP
Sbjct: 61  GFTRNWALNVSTPLTTPIFEE----------SPLEEDNKQRFDPGAPPPFNLADIRAAIP 120

Query: 121 KHCWVKDPWRSMSYVVRDVIVVFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCG 180
           KHCWVK+PW+S+SYVVRDV +VF LAA AAY NNW+VWPLYW AQGTMFWALFVLGHDCG
Sbjct: 121 KHCWVKNPWKSLSYVVRDVAIVFALAAGAAYLNNWIVWPLYWLAQGTMFWALFVLGHDCG 180

Query: 181 HGSFSNDPKLNSVVGHILHSSILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSL 240
           HGSFSNDPKLNSVVGH+LHSSILVPYHGWRISHRTHHQNHGHVENDESWHP+SEKIY +L
Sbjct: 181 HGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQNHGHVENDESWHPMSEKIYNTL 240

Query: 241 DKATRTLRFTLPFPMLAYPVYLWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAML 300
           DK TR  RFTLP  MLAYP YLW+RSPGKKGSH+ P+SDLF+P ER D++TSTACWTAM 
Sbjct: 241 DKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSHYHPDSDLFLPKERKDVLTSTACWTAMA 300

Query: 301 ALLGCLSVVMGPLQILKLYGIPYLVS 322
           ALL CL+  +GP+Q+LKLYGIPY ++
Sbjct: 301 ALLVCLNFTIGPIQMLKLYGIPYWIN 312

BLAST of Tan0022795 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 352.8 bits (904), Expect = 2.9e-97
Identity = 164/244 (67.21%), Postives = 193/244 (79.10%), Query Frame = 0

Query: 78  NERERVDGVNGVED-EGEGEFDPAAAPPFRLADIRAAIPKHCWVKDPWRSMSYVVRDVIV 137
           ++R  V+G  G  D + E  FDP+A PPF++ DIRAAIPKHCWVK P RSMSYVVRD+I 
Sbjct: 6   DQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIA 65

Query: 138 VFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHILHSS 197
           V  LA  A Y ++W +WPLYW AQGT+FWA+FVLGHDCGHGSFS+ P LNSVVGHILHS 
Sbjct: 66  VAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSF 125

Query: 198 ILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATRTLRFTLPFPMLAYPVY 257
           ILVPYHGWRISHRTHHQNHGHVENDESW PL E++Y+ L  +TR LR+T+P PMLAYP+Y
Sbjct: 126 ILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPMLAYPLY 185

Query: 258 LWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLSVVMGPLQILKLYGI 317
           L  RSPGK+GSHF+P S LF PSER  I TST CW+ M   L  LS V GPL +LK+YG+
Sbjct: 186 LCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVLKVYGV 245

Query: 318 PYLV 321
           PY++
Sbjct: 246 PYII 249

BLAST of Tan0022795 vs. TAIR 10
Match: AT2G29980.2 (fatty acid desaturase 3 )

HSP 1 Score: 352.8 bits (904), Expect = 2.9e-97
Identity = 164/244 (67.21%), Postives = 193/244 (79.10%), Query Frame = 0

Query: 78  NERERVDGVNGVED-EGEGEFDPAAAPPFRLADIRAAIPKHCWVKDPWRSMSYVVRDVIV 137
           ++R  V+G  G  D + E  FDP+A PPF++ DIRAAIPKHCWVK P RSMSYVVRD+I 
Sbjct: 6   DQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRDIIA 65

Query: 138 VFGLAAVAAYFNNWVVWPLYWFAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHILHSS 197
           V  LA  A Y ++W +WPLYW AQGT+FWA+FVLGHDCGHGSFS+ P LNSVVGHILHS 
Sbjct: 66  VAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSF 125

Query: 198 ILVPYHGWRISHRTHHQNHGHVENDESWHPLSEKIYRSLDKATRTLRFTLPFPMLAYPVY 257
           ILVPYHGWRISHRTHHQNHGHVENDESW PL E++Y+ L  +TR LR+T+P PMLAYP+Y
Sbjct: 126 ILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPMLAYPLY 185

Query: 258 LWSRSPGKKGSHFDPNSDLFVPSERNDIITSTACWTAMLALLGCLSVVMGPLQILKLYGI 317
           L  RSPGK+GSHF+P S LF PSER  I TST CW+ M   L  LS V GPL +LK+YG+
Sbjct: 186 LCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVLKVYGV 245

Query: 318 PYLV 321
           PY++
Sbjct: 246 PYII 249

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P486199.0e-13673.31Omega-3 fatty acid desaturase, chloroplastic OS=Ricinus communis OX=3988 GN=FAD7... [more]
P486202.6e-13573.23Omega-3 fatty acid desaturase, chloroplastic OS=Sesamum indicum OX=4182 GN=FAD7 ... [more]
P486212.4e-12867.91Omega-3 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 GN=FAD7 PE=2... [more]
P486222.5e-12566.25Temperature-sensitive sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloropla... [more]
P463107.2e-12565.64sn-2 acyl-lipid omega-3 desaturase (ferredoxin), chloroplastic OS=Arabidopsis th... [more]
Match NameE-valueIdentityDescription
XP_038892287.14.9e-17995.00omega-3 fatty acid desaturase, chloroplastic [Benincasa hispida][more]
XP_008465212.19.5e-17593.44PREDICTED: omega-3 fatty acid desaturase, chloroplastic [Cucumis melo] >KAA00528... [more]
NP_001274404.18.0e-17493.13omega-3 fatty acid desaturase [Cucumis sativus] >ACE80931.1 omega-3 fatty acid d... [more]
KAG7030666.11.5e-17291.88Omega-3 fatty acid desaturase, chloroplastic, partial [Cucurbita argyrosperma su... [more]
XP_022942829.11.5e-17291.88omega-3 fatty acid desaturase, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A5A7UH664.6e-17593.44Omega-3 fatty acid desaturase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3CNS04.6e-17593.44omega-3 fatty acid desaturase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350... [more]
B3VA193.9e-17493.13Omega-3 fatty acid desaturase OS=Cucumis sativus OX=3659 GN=fad7 PE=2 SV=1[more]
A0A6J1FSG47.3e-17391.88omega-3 fatty acid desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1K2M72.4e-17191.88omega-3 fatty acid desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN... [more]
Match NameE-valueIdentityDescription
AT5G05580.11.8e-12666.25fatty acid desaturase 8 [more]
AT5G05580.21.8e-12666.25fatty acid desaturase 8 [more]
AT3G11170.15.1e-12665.64fatty acid desaturase 7 [more]
AT2G29980.12.9e-9767.21fatty acid desaturase 3 [more]
AT2G29980.22.9e-9767.21fatty acid desaturase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021863Fatty acid desaturase, N-terminalPFAMPF11960DUF3474coord: 1..142
e-value: 1.9E-53
score: 180.3
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 150..326
e-value: 6.4E-17
score: 62.2
NoneNo IPR availablePANTHERPTHR32100:SF52TEMPERATURE-SENSITIVE SN-2 ACYL-LIPID OMEGA-3 DESATURASE (FERREDOXIN), CHLOROPLASTICcoord: 44..320
NoneNo IPR availablePANTHERPTHR32100OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTICcoord: 44..320
NoneNo IPR availableCDDcd03507Delta12-FADS-likecoord: 121..251
e-value: 1.26649E-56
score: 181.27

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0022795.1Tan0022795.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water