Csor.00g133400 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g133400
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	Prolyl-tRNA synthetase
Location	Csor_Chr14: 13422880 .. 13428478 (+)
RNA-Seq Expression	Csor.00g133400
Synteny	Csor.00g133400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGGTCCTAAGCCTGGTAGCTCTGCCACTAAACCAAAAGCTGGTAAATGATGTTCCTTTAATACAGCAGTTGTTTGTGCAATTGTGTCCAATTCCCTGGTTTGGCTTGTGTTTACTTGTTCTTTGTTGTTCTCTGGGCAGGCGGGAAAAAGGAGGTGAAGAAAGAGACTGGGTTGGGTCTCACCAATAAGAAGGACGACAACTTCGGAGAATGGTATTCCGAGGTGCTGTTTCGTGCACGGTTTTTGTTTTCTTCATTGCAAAATGACTCTTTATGTTCGGTAAAAATTATACATATGAGTTTGAGAATGTTGTTTGACAGTCGTCGTAGTGAAGGTAGATCGTGTAATTTCACTCGAAGGCTGTCAACGTACATGTCTCAACCCATGTGTAAAATTATTCGTATGAGTCGACCTTTTCTTTTCGAGGGATCTCCCCTGTAATATTATGAACCCGTTCTTTTCGATGACTTGAATAATTTTTTTTCTAGTTTGTCTAGGTGGTTGTCAGTGGGGAAATGATCGAGTATTACGATATCTCGGGCTGCTATATACTGAGGCCATGGGCGATGTCTATCTGGGAGACTATGCAAGTAAGGATGCTAGTCGTTCTATCGTCGATTTTACGATCTGTTCTTGTTTCGAAGTTCGAAGATTTAATTGTCATCTGTGCAGGTATTTTTTGATGCTGAAATTAAGAAAATGAAAATCAAGAATTGCTATTTTCCACTTTTTGTATCTCCCGGCGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCTGAGGTGATGTTTTATTATTCATTTATGCAATAACTCGGGATTTAATCCCATAGAGATGATAAATCTTTCGCTTGTAAATTCAATGGGCTGCATTGCATCTTGGTACTTTCAACTTCATGATCGTATTCCAGGCCGAGCACGACATCCTTATGCACCCATTCCATATGTTAGGTGTAGCTGGTGTATTCGACTCTCCCTGTTCAGTGCTATGCATGGTTCCTTGATAACTTCTAGTTTGATTAGGGAAACCACAGAAAATGAATCTGCTAATGAAGGTTATAGATTCGGTCAAGAGGAAGAAACGTATAATATCGTTGCTGCTCATGGTTATTTTTGCCGATTGATCTTCCAATATGCTAGTTTCAACAACCCTCGTTCTTTACATTTTGTCCCGTTCATCTTTTTAAAATTGATGAGAAATGGGTATTTCATTTTTATGATGAAGTTTTAAATGCATACTATCTTTGGTATGGATGTAATACCAGTTAAGTGACGATACGGTTGTCATGGTCATCGGGTTATGATTTATGAATATCATCTCGATCTTCGTGATGTCTCGATTATGTCGAGTGCCTCTCAGTTTGTTCTTCTAGCCTCTAACTTGTCAGTGTAGCTTTGGCTCAAGCGATGAACTTTTATTGCCTCCTAGTAAAAAACATTCTTAGAAAAGATTTTAGACACCCACCCTCCTCCCCTTTTGTAATAGCCCAATCCTACTGTTAGCAAATATTGTTCGTTTTGGCCCATTACGTATCGCCGTTAGCCTCACGGTTTTAAAACACGTTTGCTAGGGAGAGGTTTCCACACCCTTATAAGGAATGTTTCGTTCCCTTCTCCAACCAACGTGGGATCTCTCACACCTTTTTACCAGTTTAGTAAGAATTTATTCAATGATGAGTGATCTTTCTTGGAATTTTGTGCTTGTTTCTAAGAAGTCGTTCCATTCGTTATGTTCGGTTCGTTCATTTTTAATTTTGAAGTTTATAAAAATGCAATTGTGGTTTAGTGCTGACAATTTGGTACTTGGCATCCATTTGAAGGTTGCTTGGGTGACAAAATCTGGGGAGTCTGACTTGGAAGTGCCGATTGCGATTCGTCCTACAAGTGAGACGGTTATGTACCCGTACTATTCGAAGTGGATCAGGGGTCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAGTTCAGCCATCCCACACCATTTATTAGGCAATTACTCCTGCTTCTGCTTAATTTTAAATTACTGTATTGTTTTGAAAGAAAAGACTAATTTTCTTTAACTATCGACTGCAGGAGTCGAGAGTTCCTTTGGCAGGAAGGTCATACTGCGTTTGCTACTAAGGATGAAGCGGATACCGAGGTACAAAACTCTAGAGTTTTAAATTATGATTTAGAAACGTTTTTATCTCGAGCAAATTTGGAGATTTAATGTGAGATCCCACGTTGGTTGGAGAGGGGAACGAAACATTCTTTATAAAGGTGTGGAAACCTTTCCCTAGCAGACGCGTTTTAAAAACCTTGAGGGCTAGCCCAGAAGGGAAAGCCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACAAATGGTATAAAGAGTCAGACACAGGGTGGTGTGTCAGCAAGGACACTAAGCCCTGAAGGGGGGTGGACAACGGGCAGTGTGTCAGCGAGAATGTTGGGATCCGAAGGGGGTGGGTTGTGAGGTTCCACATCAATTGGAGAGGGGAACGAGTGCCAGCGAGGACGTTGGGCCCTGAAAAGGGTGGATTGTGAGATCTCATATTGGTTGGAGAGGGGAGCAAAGCATTCTTTATAAGAATGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAAACCTTAAGGGCTAGTCCAAAAAGGAAAGCCTAAAGAGGACAATATTTGCTAGTGATGGGCTTGAACGGTTACATTTAAACATGCAAACTCACTTTGATTGGAACCAAAAAGAATGAGTGGGGTCTAAATTGGGAGCACTAAGTTCTTTAAGTGATTCTTTTGAGATTCCACATCGGTTGGGGAGGAGAATGAAACACTCTTTATAAGGGTGCGGAAACCTCTCCCTAGCATATGCGTTTTAAAAACCTTGAGGGTAAACCCAAACGGGAAAGCCCAAAGAGGACAATATCTGCTAGCGGTGGGCTTAGGCCGTTACAAATGGGGTATTGTGAGATCCCACATCGGTTGGGGAGGAGAACGAAACACCCTTTGTTAGGGTGTGGAAACCTCTTCCTAGTAGACGCGTTTTAAAAACCTTGAGGGTAAGCCCTAAAGGGAAAACCCAAGAGGACAATATCTGCTAGCGGTGGGCTTGAGCCGTTACCAATGGTATAAGAGCCAGACACCAGACGATGTGTTGTCGAAGAGGTTGAGCCCCGAAGGGGGGTGGACATGAGGCGATGTGCCAGCAAGGACATTAGGCCCCGAAGGGGGGTGGATTTGGTGGAGGTCCCACATCGATTGGAGAAAGGAACGAGCGCCAACGAGGACCCTAGGCCCCAAAGGGGGTGGAATGTGAGATCCCACATCGGTTAGGGAGGAGAACGAAACACCCGGAAACCTCTCCCTAGCAGACACGTTTTAAAAACCTTGAGGGTAAGCTCAAAGAGGACAATATCTACCACTTATAAACGTGTGGAAACCTCTCCCTAGCAGACGCATTTTAAAAACCTCGGGGGTAAGCCCGAAAGCGAAAGCCCAAAGAGGACAATATCTACCACTTATAAAGATATGGAAACCTTTCCCTAGCAGACGCGTTTTAAAAACCTTGAGGGTAAGCTCGAAAGGGAAAGCCCAAAGAGGACAATATCTGCTAGTGGTAGGCTTGGGCCGTTAAGGATGAGTTTAAAGGGAGAATTCCAAAACATCCTTCAATCAAACCCTGAAATATACAGTTCTTTTATGAATCTGTGGTCATATTTTAAAGGTTACTTTTATTTCAGGTTCTTGAAATATTGGAATTGTATAGGCGAATATACGAAGAATACCTGGCTATTCCTGTTATCAAGGGCAAGAAGAGCGAGATGGAGAAGTTTGCCGGTGGTCTTTACACCACGAGCGTGGAGGTATAAGTTGATTAAATGTTATAGACCTGTTTTCAGCACGGTGAATCGATACCCGGTTCTCGTTTATACTGTATGAAACTGTTTCTTCTATGCAGGCATTTATCCCGAACACTGGTCGTGGAATTCAGGGTGCGACTTCACATTGCTTAGGTCAAAACTTCGCTAAAATGTTCGAAATCAACTTTGAAAACGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCGTATAGTACTAGAACGGTAATATTTTGATACTTGCATTTGTCCGAGCTTTTAGGTAACGTGTCGGAGTGAATATCTACGTCTGTTAATCTTGTCTTTCCGAATCGTTTGCAGATTGGTGTGATGGTTATGGTTCATGGGGATGACAAGGGATTGGTGCTGCCTCCAAAAGTTGCGTCGGTTCAAGTTATTATCGTTCCCGTGCCTTACAAAGATGCAGACACTCAAGGAATTTTTGATGCTTGTTCTACCACTTTGGATACATTGTCCGAAGCAGGAATTCGTGCCGAGGTCGATTCTAGGGATAATTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAAGGTGTTCCGCTTAGGATCGAAATAGGGCCGAAGGACTTGGCAAACAATCAGGTTGTTTATTCTGTCTACTGATGTTATTTTTTGTCACCATCGAAATTCGCCTTCCCTGTTACTAACACATGTATTACTTTATTTCTAGGTACGTGCCGTTCGTCGTGATAACTCGGGGAAAAAGGACATACCTAGAGCTGCATTGGTTGAACAAGTGAAAGAATTGCTCGAAAGTATTCAACAAAGCCTGTTCGACGCGGCGAAAGAAAAACGAGATGTATGCATTCAAGTTATTAATACATGGGAAGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTGGCTCCATGGTGTGATGAAGAGGTATTAAGATTTTGTTGCTTGTGTTAGCTGCTTCAGTTTTAACGTCATATTCCATTGTTTTGAACATCAGTGATCACTGGCTTGAGTAAATTGAGAGAGGTTTTCTGATATAAGAAGTTTGTAACATGTTTGCTTTATGCCAACTTTTTATCGTCTACCGGTTTCGTTTTTATGTTCCTTTAGGAGGTCGAGAAGGACGTGAAAACACGGACCAAGGGTGAGATGGGAGCAGCTAAAACCTTGTGTTCCCCGTTCGAGCAGCCCGCACTCCCAGAAGGTACTAATTTCTGTCCAAATCTGTATTTGGTTCTGTAACAATCCAAGCCCATTGCTATAGCCGATATGGTTCTCTTTGAGTTTTTTTTTCCTTTAGAGCTTGCGTTCAAGGTTTTTAAAATGTGTCTGCTAGGGAGGGGTTTCCACACCCTTATAAAAAATGCTTCGTTCTCCTCCCCAATTGAGTGTCCTCGCTGGGCTCCGAATGGTAGTAGATTGTGAGATTCCACATCGGTTGGGAGGAGAATGAAGCATAAGGGTGTGAAAACGTCTCCCTAGTAGACGTGTTTTAAAAACTGTGAGCGGACAATATCTGCTATTGGTGGAGTGGGCTTGGGCCGTTACAGTTTTTGATGGTGAGTTGTTGGGAAAATTATTAGGAACTCTTTTTGGTTTCCTTGAATTTACGGTGATGTTCGTGGTCAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGTCGAAGCTACTAA

mRNA sequence

ATGGCTGGTCCTAAGCCTGGTAGCTCTGCCACTAAACCAAAAGCTGGCGGGAAAAAGGAGGTGAAGAAAGAGACTGGGTTGGGTCTCACCAATAAGAAGGACGACAACTTCGGAGAATGGTATTCCGAGGTGGTTGTCAGTGGGGAAATGATCGAGTATTACGATATCTCGGGCTGCTATATACTGAGGCCATGGGCGATGTCTATCTGGGAGACTATGCAAGTATTTTTTGATGCTGAAATTAAGAAAATGAAAATCAAGAATTGCTATTTTCCACTTTTTGTATCTCCCGGCGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCTGAGGTTGCTTGGGTGACAAAATCTGGGGAGTCTGACTTGGAAGTGCCGATTGCGATTCGTCCTACAAGTGAGACGGTTATGTACCCGTACTATTCGAAGTGGATCAGGGGTCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAGTTCAGCCATCCCACACCATTTATTAGGAGTCGAGAGTTCCTTTGGCAGGAAGGTCATACTGCGTTTGCTACTAAGGATGAAGCGGATACCGAGGTTCTTGAAATATTGGAATTGTATAGGCGAATATACGAAGAATACCTGGCTATTCCTGTTATCAAGGGCAAGAAGAGCGAGATGGAGAAGTTTGCCGGTGGTCTTTACACCACGAGCGTGGAGGCATTTATCCCGAACACTGGTCGTGGAATTCAGGGTGCGACTTCACATTGCTTAGGTCAAAACTTCGCTAAAATGTTCGAAATCAACTTTGAAAACGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCGTATAGTACTAGAACGATTGGTGTGATGGTTATGGTTCATGGGGATGACAAGGGATTGGTGCTGCCTCCAAAAGTTGCGTCGGTTCAAGTTATTATCGTTCCCGTGCCTTACAAAGATGCAGACACTCAAGGAATTTTTGATGCTTGTTCTACCACTTTGGATACATTGTCCGAAGCAGGAATTCGTGCCGAGGTCGATTCTAGGGATAATTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAAGGTGTTCCGCTTAGGATCGAAATAGGGCCGAAGGACTTGGCAAACAATCAGGTACGTGCCGTTCGTCGTGATAACTCGGGGAAAAAGGACATACCTAGAGCTGCATTGGTTGAACAAGTGAAAGAATTGCTCGAAAGTATTCAACAAAGCCTGTTCGACGCGGCGAAAGAAAAACGAGATGTATGCATTCAAGTTATTAATACATGGGAAGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTGGCTCCATGGTGTGATGAAGAGGAGGTCGAGAAGGACGTGAAAACACGGACCAAGGGTGAGATGGGAGCAGCTAAAACCTTGTGTTCCCCGTTCGAGCAGCCCGCACTCCCAGAAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGTCGAAGCTACTAA

Coding sequence (CDS)

ATGGCTGGTCCTAAGCCTGGTAGCTCTGCCACTAAACCAAAAGCTGGCGGGAAAAAGGAGGTGAAGAAAGAGACTGGGTTGGGTCTCACCAATAAGAAGGACGACAACTTCGGAGAATGGTATTCCGAGGTGGTTGTCAGTGGGGAAATGATCGAGTATTACGATATCTCGGGCTGCTATATACTGAGGCCATGGGCGATGTCTATCTGGGAGACTATGCAAGTATTTTTTGATGCTGAAATTAAGAAAATGAAAATCAAGAATTGCTATTTTCCACTTTTTGTATCTCCCGGCGTCTTACAAAGAGAGAAAGATCATATAGAAGGTTTTGCTCCTGAGGTTGCTTGGGTGACAAAATCTGGGGAGTCTGACTTGGAAGTGCCGATTGCGATTCGTCCTACAAGTGAGACGGTTATGTACCCGTACTATTCGAAGTGGATCAGGGGTCACCGTGACTTGCCTTTGAAACTTAACCAGTGGTGCAATGTTGTGAGATGGGAGTTCAGCCATCCCACACCATTTATTAGGAGTCGAGAGTTCCTTTGGCAGGAAGGTCATACTGCGTTTGCTACTAAGGATGAAGCGGATACCGAGGTTCTTGAAATATTGGAATTGTATAGGCGAATATACGAAGAATACCTGGCTATTCCTGTTATCAAGGGCAAGAAGAGCGAGATGGAGAAGTTTGCCGGTGGTCTTTACACCACGAGCGTGGAGGCATTTATCCCGAACACTGGTCGTGGAATTCAGGGTGCGACTTCACATTGCTTAGGTCAAAACTTCGCTAAAATGTTCGAAATCAACTTTGAAAACGAAAAGGGGGAGAAAGCTATGGTCTGGCAAAATTCGTGGGCGTATAGTACTAGAACGATTGGTGTGATGGTTATGGTTCATGGGGATGACAAGGGATTGGTGCTGCCTCCAAAAGTTGCGTCGGTTCAAGTTATTATCGTTCCCGTGCCTTACAAAGATGCAGACACTCAAGGAATTTTTGATGCTTGTTCTACCACTTTGGATACATTGTCCGAAGCAGGAATTCGTGCCGAGGTCGATTCTAGGGATAATTATTCTCCTGGATGGAAGTATTCTCACTGGGAAATGAAAGGTGTTCCGCTTAGGATCGAAATAGGGCCGAAGGACTTGGCAAACAATCAGGTACGTGCCGTTCGTCGTGATAACTCGGGGAAAAAGGACATACCTAGAGCTGCATTGGTTGAACAAGTGAAAGAATTGCTCGAAAGTATTCAACAAAGCCTGTTCGACGCGGCGAAAGAAAAACGAGATGTATGCATTCAAGTTATTAATACATGGGAAGAGTTTACTGAAGCACTCAGTCAGAAGAAAATGATATTGGCTCCATGGTGTGATGAAGAGGAGGTCGAGAAGGACGTGAAAACACGGACCAAGGGTGAGATGGGAGCAGCTAAAACCTTGTGTTCCCCGTTCGAGCAGCCCGCACTCCCAGAAGGTACCAAATGTTTTGCATCTGGGAAGCCTGCAAAGAAATGGAGCTATTGGGGTCGAAGCTACTAA

Protein sequence

MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY

Homology

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BLAST of Csor.00g133400 vs. ExPASy Swiss-Prot
Match: Q9M1R2 (Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 PE=1 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 9.4e-257
Identity = 412/493 (83.57%), Postives = 455/493 (92.29%), Query Frame = 0


Query: 18  KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVFF 77
           KK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE MQ+FF
Sbjct: 39  KKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIMQIFF 98

Query: 78  DAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSET 137
           DAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRPTSET
Sbjct: 99  DAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRPTSET 158

Query: 138 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKDEADT 197
           VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSREFLWQEGHTAFATK EAD 
Sbjct: 159 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKAEADE 218

Query: 198 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 257
           EVL+ILELYRRIYEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCL
Sbjct: 219 EVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCL 278

Query: 258 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVII 317
           GQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASVQV++
Sbjct: 279 GQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVV 338

Query: 318 VPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIG 377
           +PVPYKDA+TQGI+DAC+ T   L EAGIRAE D RDNYSPGWKYS WEMKGVPLRIEIG
Sbjct: 339 IPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIG 398

Query: 378 PKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLFDAAKEKRDVCIQVINTW 437
           P+DL N+QVR VRRDN  K+DIPR +LVE VKELLE IQQ++++ AK+KR+ C+Q + TW
Sbjct: 399 PRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTW 458

Query: 438 EEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASG 497
           +EF +AL++KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASG
Sbjct: 459 DEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASG 518

Query: 498 KPAKKWSYWGRSY 511
           KPAKKW+YWGRSY
Sbjct: 519 KPAKKWTYWGRSY 530

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BLAST of Csor.00g133400 vs. ExPASy Swiss-Prot
Match: Q8CGC7 (Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE=1 SV=4)

HSP 1 Score: 578.2 bits (1489), Expect = 9.3e-164
Identity = 290/519 (55.88%), Postives = 370/519 (71.29%), Query Frame = 0


Query: 7    GSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWA 66
            GS  +   AG  +  KK+T LGL  KK++N  EWYS+V+   EMIEYYD+SGCYILRPW+
Sbjct: 994  GSGLSSGGAGEGQGPKKQTRLGLEAKKEENLAEWYSQVITKSEMIEYYDVSGCYILRPWS 1053

Query: 67   MSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLE 126
             SIWE+++ FFDAEIKK+ ++NCYFP+FVS   L++EK+HIE FAPEVAWVT+SG+++L 
Sbjct: 1054 YSIWESIKDFFDAEIKKLGVENCYFPIFVSQAALEKEKNHIEDFAPEVAWVTRSGKTELA 1113

Query: 127  VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGH 186
             PIAIRPTSETVMYP Y+KW++ HRDLP++LNQWCNVVRWEF HP PF+R+REFLWQEGH
Sbjct: 1114 EPIAIRPTSETVMYPAYAKWVQSHRDLPVRLNQWCNVVRWEFKHPQPFLRTREFLWQEGH 1173

Query: 187  TAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTG 246
            +AFAT +EA  EVL+ILELY R+YEE LAIPV++G+K+E EKFAGG YTT++EAFI  +G
Sbjct: 1174 SAFATFEEAADEVLQILELYARVYEELLAIPVVRGRKTEKEKFAGGDYTTTIEAFISASG 1233

Query: 247  RGIQGATSHCLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDDKGL 306
            R IQGATSH LGQNF+KM EI FE+ K  GEK   +Q SW  +TRTIGVMVMVHGD+ GL
Sbjct: 1234 RAIQGATSHHLGQNFSKMCEIVFEDPKTPGEKQFAYQCSWGLTTRTIGVMVMVHGDNMGL 1293

Query: 307  VLPPKVASVQVIIVPVPYKDA----DTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 366
            VLPP+VASVQV+++P    +A    D + +   C+     L  A IR  VD RDNYSPGW
Sbjct: 1294 VLPPRVASVQVVVIPCGITNALSEEDREALMAKCNEYRRRLLGANIRVRVDLRDNYSPGW 1353

Query: 367  KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 426
            K++HWE+KGVP+R+E+GP+D+ + Q  AVRRD   K  I       +++++LE IQ +LF
Sbjct: 1354 KFNHWELKGVPVRLEVGPRDMKSCQFVAVRRDTGEKLTIAEKEAEAKLEKVLEDIQLNLF 1413

Query: 427  DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKG----EMGA-- 486
              A E     + V NT E+F + L   K+   P+C E + E  +K  T      E GA  
Sbjct: 1414 TRASEDLKTHMVVSNTLEDFQKVLDAGKVAQIPFCGEIDCEDWIKKMTARDQDVEPGAPS 1473

Query: 487  --AKTLCSPFEQPA-LPEGTKCFASGKPAKKWSYWGRSY 511
              AK+LC PF     L  G  C     PAK ++ +GRSY
Sbjct: 1474 MGAKSLCIPFNPLCELQPGAMCVCGKNPAKFYTLFGRSY 1512

[Close]

BLAST of Csor.00g133400 vs. ExPASy Swiss-Prot
Match: O60155 (Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=prs1 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 3.5e-163
Identity = 294/546 (53.85%), Postives = 369/546 (67.58%), Query Frame = 0


Query: 2   AGPKPGSSATKPKAGGKK--------EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEY 61
           + P   ++ +KP A  KK         ++    +G+T +KD +F  WY +V+   +MIEY
Sbjct: 172 SAPGSATAPSKPAAQKKKAEPSKNDAAIENAALIGITVRKDADFPNWYQQVLTKSDMIEY 231

Query: 62  YDISGCYILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPE 121
           YDISGCYIL+PW+ SIWE +Q +FD EIKK+ ++N YFPLFVS  VL++EKDH+EGFAPE
Sbjct: 232 YDISGCYILKPWSYSIWEAIQGWFDKEIKKLGVRNGYFPLFVSSKVLEKEKDHVEGFAPE 291

Query: 122 VAWVTKSGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTP 181
           VAWVT++G S+L+ PIAIRPTSETVMYPYY+KWIR HRDLPLKLNQW +VVRWEF +P P
Sbjct: 292 VAWVTRAGTSELDEPIAIRPTSETVMYPYYAKWIRSHRDLPLKLNQWNSVVRWEFKNPQP 351

Query: 182 FIRSREFLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGL 241
           F+R+REFLWQEGHTA  T + A  EV +IL+LY RIY + LA+PVIKG KSE EKFAGG+
Sbjct: 352 FLRTREFLWQEGHTAHMTLEGATEEVHQILDLYARIYTDLLAVPVIKGVKSENEKFAGGM 411

Query: 242 YTTSVEAFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGE--------KAMVWQNSWA 301
           +TT+VE +IP TGRGIQGATSHCLGQNF+KMF I  E+   E        K  VWQNSW 
Sbjct: 412 FTTTVEGYIPTTGRGIQGATSHCLGQNFSKMFNIVVEDPNAEIGPTGERPKLFVWQNSWG 471

Query: 302 YSTRTIGVMVMVHGDDKGLVLPPKVASVQVIIVPV----PYKDADTQGIFDACSTTLDTL 361
            STRTIGV VMVHGDDKGL LPP +A VQ ++VP        D +   I   CS   D L
Sbjct: 472 LSTRTIGVAVMVHGDDKGLKLPPAIALVQSVVVPCGITNKTTDQERNEIEGFCSKLADRL 531

Query: 362 SEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPR 421
           + A IR E D R  Y+PG+K+SHWEMKGVPLR+E GP D   NQV AVRRD   K  +P 
Sbjct: 532 NAADIRTEADLR-AYTPGYKFSHWEMKGVPLRLEYGPNDAKKNQVTAVRRDTFEKIPVPL 591

Query: 422 AALVEQVKELLESIQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVE 481
             L + V +LL  IQ ++++ AK +RD  +  +  W +F  AL++K +++ PWC+  E E
Sbjct: 592 NNLEKGVSDLLAKIQTNMYETAKAERDAHVVKVKEWADFVPALNKKNIVMIPWCNTTECE 651

Query: 482 KDVKTRT------------KGEMGAAKTLCSPFEQP----ALPEG-TKCFASGKPAKKWS 511
           K++K  +            K     AK+LC P EQP    A+ EG TKC   G  AK W 
Sbjct: 652 KEIKKNSARQVNGDEPEDEKAPSMGAKSLCIPLEQPSGEDAIIEGTTKCAGCGNLAKVWG 711

[Close]

BLAST of Csor.00g133400 vs. ExPASy Swiss-Prot
Match: P28668 (Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 GN=GluProRS PE=1 SV=2)

HSP 1 Score: 575.9 bits (1483), Expect = 4.6e-163
Identity = 292/527 (55.41%), Postives = 373/527 (70.78%), Query Frame = 0


Query: 2    AGPKPGSSATK-PKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 61
            A PKP     K P A     VKK+T LGL   K+DN  +WYS+V+  GEMIEYYD+SGCY
Sbjct: 1189 AQPKPAKPVKKEPAADASGAVKKQTRLGLEATKEDNLPDWYSQVITKGEMIEYYDVSGCY 1248

Query: 62   ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 121
            ILR W+ +IW+ ++ +FDAEI +M +K CYFP+FVS  VL++EK HI  FAPEVAWVTKS
Sbjct: 1249 ILRQWSFAIWKAIKTWFDAEITRMGVKECYFPIFVSKAVLEKEKTHIADFAPEVAWVTKS 1308

Query: 122  GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 181
            G+SDL  PIA+RPTSETVMYP Y+KW++ +RDLP++LNQW NVVRWEF  PTPF+R+REF
Sbjct: 1309 GDSDLAEPIAVRPTSETVMYPAYAKWVQSYRDLPIRLNQWNNVVRWEFKQPTPFLRTREF 1368

Query: 182  LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 241
            LWQEGHTAFA K+EA  EVL+IL+LY  +Y   LAIPV+KG+K+E EKFAGG YTT+VEA
Sbjct: 1369 LWQEGHTAFADKEEAAKEVLDILDLYALVYTHLLAIPVVKGRKTEKEKFAGGDYTTTVEA 1428

Query: 242  FIPNTGRGIQGATSHCLGQNFAKMFEINFEN-EKGEKAMVWQNSWAYSTRTIGVMVMVHG 301
            FI  +GR IQGATSH LGQNF+KMFEI +E+ E  +K  V+QNSW  +TRTIGVM+MVH 
Sbjct: 1429 FISASGRAIQGATSHHLGQNFSKMFEIVYEDPETQQKKYVYQNSWGITTRTIGVMIMVHA 1488

Query: 302  DDKGLVLPPKVASVQVIIVP----VPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDN 361
            D++GLVLPP VA +Q I+VP    V  KD +   + DAC      L   G+R E D RDN
Sbjct: 1489 DNQGLVLPPHVACIQAIVVPCGITVNTKDDERAQLLDACKALEKRLVGGGVRCEGDYRDN 1548

Query: 362  YSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESI 421
            YSPGWK++HWE+KGVPLR+E+GPKDL   Q+ AVRRD   K  IP A + +++  LLE+I
Sbjct: 1549 YSPGWKFNHWELKGVPLRLEVGPKDLKAQQLVAVRRDTVEKITIPLADVEKKIPALLETI 1608

Query: 422  QQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRT----KGE 481
             +S+ + A+E      + +  W +F   L QK ++LAP+C E   E  +K  +    + E
Sbjct: 1609 HESMLNKAQEDMTSHTKKVTNWTDFCGFLEQKNILLAPFCGEISCEDKIKADSARGEEAE 1668

Query: 482  MGA----AKTLCSPFEQPA-LPEGTKCF---ASGKPAKKWSYWGRSY 511
             GA    AK+LC PF+QPA +    KC     + KP K ++ +GRSY
Sbjct: 1669 PGAPAMGAKSLCIPFDQPAPIAASDKCINPSCTNKP-KFYTLFGRSY 1714

[Close]

BLAST of Csor.00g133400 vs. ExPASy Swiss-Prot
Match: P07814 (Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=1 SV=5)

HSP 1 Score: 575.5 bits (1482), Expect = 6.1e-163
Identity = 284/519 (54.72%), Postives = 365/519 (70.33%), Query Frame = 0


Query: 7    GSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWA 66
            G   +   AG  +  KK+T LGL  KK++N  +WYS+V+   EMIEY+DISGCYILRPWA
Sbjct: 994  GGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWA 1053

Query: 67   MSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLE 126
             +IWE ++ FFDAEIKK+ ++NCYFP+FVS   L++EK H+  FAPEVAWVT+SG+++L 
Sbjct: 1054 YAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELA 1113

Query: 127  VPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGH 186
             PIAIRPTSETVMYP Y+KW++ HRDLP+KLNQWCNVVRWEF HP PF+R+REFLWQEGH
Sbjct: 1114 EPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGH 1173

Query: 187  TAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTG 246
            +AFAT +EA  EVL+IL+LY ++YEE LAIPV+KG+K+E EKFAGG YTT++EAFI  +G
Sbjct: 1174 SAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASG 1233

Query: 247  RGIQGATSHCLGQNFAKMFEINFENEK--GEKAMVWQNSWAYSTRTIGVMVMVHGDDKGL 306
            R IQG TSH LGQNF+KMFEI FE+ K  GEK   +QNSW  +TRTIGVM MVHGD+ GL
Sbjct: 1234 RAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGL 1293

Query: 307  VLPPKVASVQVIIVPVPYKDA----DTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 366
            VLPP+VA VQV+I+P    +A    D + +   C+     L    IR   D RDNYSPGW
Sbjct: 1294 VLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGW 1353

Query: 367  KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 426
            K++HWE+KGVP+R+E+GP+D+ + Q  AVRRD   K  +       +++ +LE IQ +LF
Sbjct: 1354 KFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLF 1413

Query: 427  DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKG----EMGA-- 486
              A E     + V NT E+F + L   K++  P+C E + E  +K  T      E GA  
Sbjct: 1414 TRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPS 1473

Query: 487  --AKTLCSPFEQPA-LPEGTKCFASGKPAKKWSYWGRSY 511
              AK+LC PF+    L  G KC     PAK ++ +GRSY
Sbjct: 1474 MGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY 1512

[Close]

BLAST of Csor.00g133400 vs. NCBI nr
Match: XP_022955643.1 (proline--tRNA ligase, cytoplasmic isoform X1 [Cucurbita moschata] >KAG6582053.1 Proline--tRNA ligase, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1053 bits (2723), Expect = 0.0
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0


Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY
Sbjct: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS
Sbjct: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF
Sbjct: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180

Query: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA
Sbjct: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
           FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD
Sbjct: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360
           DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW
Sbjct: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360

Query: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 420
           KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF
Sbjct: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 420

Query: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480
           DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS
Sbjct: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480

Query: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
           PFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

[Close]

BLAST of Csor.00g133400 vs. NCBI nr
Match: XP_023528664.1 (proline--tRNA ligase, cytoplasmic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1051 bits (2717), Expect = 0.0
Identity = 509/510 (99.80%), Postives = 509/510 (99.80%), Query Frame = 0


Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY
Sbjct: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS
Sbjct: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF
Sbjct: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180

Query: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA
Sbjct: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
           FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD
Sbjct: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360
           DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW
Sbjct: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360

Query: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 420
           KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF
Sbjct: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 420

Query: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480
           DAAKEKRDVCIQVI TWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS
Sbjct: 421 DAAKEKRDVCIQVITTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480

Query: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
           PFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

[Close]

BLAST of Csor.00g133400 vs. NCBI nr
Match: XP_022979761.1 (proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima])

HSP 1 Score: 1045 bits (2701), Expect = 0.0
Identity = 505/510 (99.02%), Postives = 508/510 (99.61%), Query Frame = 0


Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY
Sbjct: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS
Sbjct: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF
Sbjct: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180

Query: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTAFATKDEAD EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA
Sbjct: 181 LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
           FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD
Sbjct: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360
           DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACS TLDTLS+AGIRAEVDSRDNYSPGW
Sbjct: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 360

Query: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 420
           KYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPRA+LVEQVKELLESIQQSLF
Sbjct: 361 KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF 420

Query: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480
           DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS
Sbjct: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480

Query: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
           PFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

[Close]

BLAST of Csor.00g133400 vs. NCBI nr
Match: XP_038907011.1 (proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907020.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907029.1 proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1033 bits (2670), Expect = 0.0
Identity = 502/511 (98.24%), Postives = 504/511 (98.63%), Query Frame = 0


Query: 1   MAGPKPGSSATKPKAGGKK-EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSAT PKAGGKK EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYR IYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRCIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACS TLDTLSEAGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSEAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPR +LVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAKEKRD CIQVINTWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKEKRDKCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
           SPFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511

[Close]

BLAST of Csor.00g133400 vs. NCBI nr
Match: XP_023543704.1 (proline--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1031 bits (2665), Expect = 0.0
Identity = 500/511 (97.85%), Postives = 503/511 (98.43%), Query Frame = 0


Query: 1   MAGPKPGSSATKPKAGGKK-EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSA  PKAGGKK EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACS TLDTLSEAGIRAEVD+RDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRA+LVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLESIQQSL 420

Query: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
           SPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

[Close]

BLAST of Csor.00g133400 vs. ExPASy TrEMBL
Match: A0A6J1GUJ5 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1)

HSP 1 Score: 1053 bits (2723), Expect = 0.0
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0


Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY
Sbjct: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS
Sbjct: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF
Sbjct: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180

Query: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA
Sbjct: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
           FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD
Sbjct: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360
           DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW
Sbjct: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360

Query: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 420
           KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF
Sbjct: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 420

Query: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480
           DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS
Sbjct: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480

Query: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
           PFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

[Close]

BLAST of Csor.00g133400 vs. ExPASy TrEMBL
Match: A0A6J1IRP0 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1)

HSP 1 Score: 1045 bits (2701), Expect = 0.0
Identity = 505/510 (99.02%), Postives = 508/510 (99.61%), Query Frame = 0


Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY
Sbjct: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS
Sbjct: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF
Sbjct: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180

Query: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTAFATKDEAD EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA
Sbjct: 181 LWQEGHTAFATKDEADAEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
           FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD
Sbjct: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360
           DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACS TLDTLS+AGIRAEVDSRDNYSPGW
Sbjct: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLSKAGIRAEVDSRDNYSPGW 360

Query: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLF 420
           KYSHWEMKGVPLRIEIGPKDLA+NQVRAVRRDNSGKKDIPRA+LVEQVKELLESIQQSLF
Sbjct: 361 KYSHWEMKGVPLRIEIGPKDLASNQVRAVRRDNSGKKDIPRASLVEQVKELLESIQQSLF 420

Query: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480
           DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS
Sbjct: 421 DAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCS 480

Query: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
           PFEQPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 PFEQPALPEGTKCFASGKPAKKWSYWGRSY 510

[Close]

BLAST of Csor.00g133400 vs. ExPASy TrEMBL
Match: A0A6J1GYJ1 (Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1)

HSP 1 Score: 1030 bits (2662), Expect = 0.0
Identity = 499/511 (97.65%), Postives = 503/511 (98.43%), Query Frame = 0


Query: 1   MAGPKPGSSATKPKAGGKK-EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSA  PKAGGKK EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACS TLDTLSEAGIRAEVD+RDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRA+LVEQVKELL+SIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLDSIQQSL 420

Query: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAK KRD CIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
           SPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

[Close]

BLAST of Csor.00g133400 vs. ExPASy TrEMBL
Match: A0A6J1JLY7 (Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111487085 PE=3 SV=1)

HSP 1 Score: 1028 bits (2658), Expect = 0.0
Identity = 498/511 (97.46%), Postives = 502/511 (98.24%), Query Frame = 0


Query: 1   MAGPKPGSSATKPKAGGKK-EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSA  PKAGGKK EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSAKTPKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQV+IVPVPYKDADTQGIFDACS TLDTLSEAGIRAEVD+RDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVVIVPVPYKDADTQGIFDACSVTLDTLSEAGIRAEVDARDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNS KKDIPRA+LVEQVKELL+SIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSAKKDIPRASLVEQVKELLDSIQQSL 420

Query: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAK KRD CIQVINTWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
           SPFEQP LPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFEQPELPEGTKCFASGKPAKKWSYWGRSY 511

[Close]

BLAST of Csor.00g133400 vs. ExPASy TrEMBL
Match: A0A0A0KTZ1 (Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1)

HSP 1 Score: 1025 bits (2649), Expect = 0.0
Identity = 497/511 (97.26%), Postives = 502/511 (98.24%), Query Frame = 0


Query: 1   MAGPKPGSSATKPKAGGKK-EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60
           MAGPKPGSSAT  KAGGKK EVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC
Sbjct: 1   MAGPKPGSSATNKKAGGKKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGC 60

Query: 61  YILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120
           YILRPWA+SIWETMQVFFDAEIK+MKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK
Sbjct: 61  YILRPWAISIWETMQVFFDAEIKQMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTK 120

Query: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180
           SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE
Sbjct: 121 SGESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSRE 180

Query: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240
           FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE
Sbjct: 181 FLWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVE 240

Query: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300
           AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG
Sbjct: 241 AFIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHG 300

Query: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPG 360
           DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACS TLDTL+ AGIRAEVDSRDNYSPG
Sbjct: 301 DDKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSATLDTLTAAGIRAEVDSRDNYSPG 360

Query: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSL 420
           WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPR +LVEQVKELLESIQQSL
Sbjct: 361 WKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRDSLVEQVKELLESIQQSL 420

Query: 421 FDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480
           FDAAK KRD CIQVINTWEEFTEAL QKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC
Sbjct: 421 FDAAKVKRDTCIQVINTWEEFTEALGQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLC 480

Query: 481 SPFEQPALPEGTKCFASGKPAKKWSYWGRSY 510
           SPF+QPALPEGTKCFASGKPAKKWSYWGRSY
Sbjct: 481 SPFDQPALPEGTKCFASGKPAKKWSYWGRSY 511

[Close]

BLAST of Csor.00g133400 vs. TAIR 10
Match: AT3G62120.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 887.1 bits (2291), Expect = 6.7e-258
Identity = 412/493 (83.57%), Postives = 455/493 (92.29%), Query Frame = 0


Query: 18  KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVFF 77
           KK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE MQ+FF
Sbjct: 39  KKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIMQIFF 98

Query: 78  DAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSET 137
           DAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRPTSET
Sbjct: 99  DAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRPTSET 158

Query: 138 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKDEADT 197
           VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSREFLWQEGHTAFATK EAD 
Sbjct: 159 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKAEADE 218

Query: 198 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 257
           EVL+ILELYRRIYEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCL
Sbjct: 219 EVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCL 278

Query: 258 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVII 317
           GQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASVQV++
Sbjct: 279 GQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVV 338

Query: 318 VPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIG 377
           +PVPYKDA+TQGI+DAC+ T   L EAGIRAE D RDNYSPGWKYS WEMKGVPLRIEIG
Sbjct: 339 IPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIG 398

Query: 378 PKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLFDAAKEKRDVCIQVINTW 437
           P+DL N+QVR VRRDN  K+DIPR +LVE VKELLE IQQ++++ AK+KR+ C+Q + TW
Sbjct: 399 PRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTW 458

Query: 438 EEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASG 497
           +EF +AL++KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASG
Sbjct: 459 DEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASG 518

Query: 498 KPAKKWSYWGRSY 511
           KPAKKW+YWGRSY
Sbjct: 519 KPAKKWTYWGRSY 530

[Close]

BLAST of Csor.00g133400 vs. TAIR 10
Match: AT3G62120.2 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 887.1 bits (2291), Expect = 6.7e-258
Identity = 412/493 (83.57%), Postives = 455/493 (92.29%), Query Frame = 0


Query: 18  KKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCYILRPWAMSIWETMQVFF 77
           KK+VKKETGLGL+ KKD+NFGEWYSE V   +MIEYYDISGCYILRPW+M+IWE MQ+FF
Sbjct: 39  KKDVKKETGLGLSVKKDENFGEWYSE-VCKQDMIEYYDISGCYILRPWSMAIWEIMQIFF 98

Query: 78  DAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKSGESDLEVPIAIRPTSET 137
           DAEIKKMK+KNCYFPLFVSPGVL++EKDHIEGFAPEVAWVTKSG+SDLEVPIAIRPTSET
Sbjct: 99  DAEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRPTSET 158

Query: 138 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREFLWQEGHTAFATKDEADT 197
           VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFS+PTPFIRSREFLWQEGHTAFATK EAD 
Sbjct: 159 VMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSNPTPFIRSREFLWQEGHTAFATKAEADE 218

Query: 198 EVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEAFIPNTGRGIQGATSHCL 257
           EVL+ILELYRRIYEEYLA+PV+KG KSE EKFAGGLYTTSVEAFIPNTGRG+QGATSHCL
Sbjct: 219 EVLQILELYRRIYEEYLAVPVVKGMKSENEKFAGGLYTTSVEAFIPNTGRGVQGATSHCL 278

Query: 258 GQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGDDKGLVLPPKVASVQVII 317
           GQNFAKMFEINFENEK E  MVWQNSWAYSTRTIGVM+M HGDDKGLVLPPKVASVQV++
Sbjct: 279 GQNFAKMFEINFENEKAETEMVWQNSWAYSTRTIGVMIMTHGDDKGLVLPPKVASVQVVV 338

Query: 318 VPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGWKYSHWEMKGVPLRIEIG 377
           +PVPYKDA+TQGI+DAC+ T   L EAGIRAE D RDNYSPGWKYS WEMKGVPLRIEIG
Sbjct: 339 IPVPYKDANTQGIYDACTATASALCEAGIRAEEDLRDNYSPGWKYSDWEMKGVPLRIEIG 398

Query: 378 PKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLESIQQSLFDAAKEKRDVCIQVINTW 437
           P+DL N+QVR VRRDN  K+DIPR +LVE VKELLE IQQ++++ AK+KR+ C+Q + TW
Sbjct: 399 PRDLENDQVRTVRRDNGVKEDIPRGSLVEHVKELLEKIQQNMYEVAKQKREACVQEVKTW 458

Query: 438 EEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGAAKTLCSPFEQPALPEGTKCFASG 497
           +EF +AL++KK+ILAPWCDEEEVE+DVK RTKGE GAAKTLCSPF+QP LPEGT CFASG
Sbjct: 459 DEFIKALNEKKLILAPWCDEEEVERDVKARTKGETGAAKTLCSPFDQPELPEGTLCFASG 518

Query: 498 KPAKKWSYWGRSY 511
           KPAKKW+YWGRSY
Sbjct: 519 KPAKKWTYWGRSY 530

[Close]

BLAST of Csor.00g133400 vs. TAIR 10
Match: AT5G52520.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 417.5 bits (1072), Expect = 1.5e-116
Identity = 214/517 (41.39%), Postives = 311/517 (60.15%), Query Frame = 0


Query: 1   MAGPKPGSSATKPKAGGKKEVKKETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYDISGCY 60
           ++G     S T        EV +         +  +F  WY +V+ S E+ +Y  + G  
Sbjct: 37  LSGFATAPSGTASPETKSSEVDRLRSDRAVTPRSQDFNAWYLDVIASAELADYGPVRGTM 96

Query: 61  ILRPWAMSIWETMQVFFDAEIKKMKIKNCYFPLFVSPGVLQREKDHIEGFAPEVAWVTKS 120
           ++RP+  +IWE +Q + + + K+    N YFP F+    +++E  H+EGF+PE+A VT  
Sbjct: 97  VIRPYGYAIWEAIQDYLNVKFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVG 156

Query: 121 GESDLEVPIAIRPTSETVMYPYYSKWIRGHRDLPLKLNQWCNVVRWEFSHPTPFIRSREF 180
           G  +LE  + +RPTSET++   +++WI  +RDLPL +NQW NV RWE     PFIR+ EF
Sbjct: 157 GGKELEEKLVVRPTSETIVNHMFTQWIHSYRDLPLMINQWANVTRWEM-RTKPFIRTLEF 216

Query: 181 LWQEGHTAFATKDEADTEVLEILELYRRIYEEYLAIPVIKGKKSEMEKFAGGLYTTSVEA 240
           LWQEGHTA AT +EA+ E  +++E+Y R   E  AIPVI G+KS++E FAG   T ++EA
Sbjct: 217 LWQEGHTAHATPEEAEKEAKQMIEIYTRFAFEQTAIPVIPGRKSKLETFAGADITYTIEA 276

Query: 241 FIPNTGRGIQGATSHCLGQNFAKMFEINFENEKGEKAMVWQNSWAYSTRTIGVMVMVHGD 300
            + +  + +Q  TSH LGQNF++ F   F +E GE+  VWQ SWA STR +G ++M HGD
Sbjct: 277 MMGDR-KALQAGTSHNLGQNFSRAFGTQFADENGERQHVWQTSWAVSTRFVGGIIMTHGD 336

Query: 301 DKGLVLPPKVASVQVIIVPVPYKDADTQGIFDACSTTLDTLSEAGIRAEVDSRDNYSPGW 360
           D GL+LPPK+A +QV+IVP+  KD +  G+  A S+  + L  AG+R ++D  D  +PGW
Sbjct: 337 DTGLMLPPKIAPIQVVIVPIWKKDTEKTGVLSAASSVKEALQTAGVRVKLDDTDQRTPGW 396

Query: 361 KYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKK------DIPRAALVEQVKELLES 420
           K++ WEMKG+PLRIEIGP+D+++N V   RRD  GK        +  + LV  VKE L+ 
Sbjct: 397 KFNFWEMKGIPLRIEIGPRDVSSNSVVVSRRDVPGKAGKVFGISMEPSTLVAYVKEKLDE 456

Query: 421 IQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEMGA 480
           IQ SL + A   RD  I  +N++ E  +A+S  K    PW   +  E+ VK  T      
Sbjct: 457 IQTSLLEKALSFRDSNIVDVNSYAELKDAISSGKWARGPWSASDADEQRVKEET-----G 516

Query: 481 AKTLCSPFEQPALPEGTK-CFASGKPAKKWSYWGRSY 511
           A   C PFEQ    +GTK C  +G PA++ + + +SY
Sbjct: 517 ATIRCFPFEQ---TQGTKTCLMTGNPAEEVAIFAKSY 543

[Close]

BLAST of Csor.00g133400 vs. TAIR 10
Match: AT5G10880.1 (tRNA synthetase-related / tRNA ligase-related )

HSP 1 Score: 289.3 bits (739), Expect = 6.2e-78
Identity = 144/221 (65.16%), Postives = 165/221 (74.66%), Query Frame = 0


Query: 296 MVHGDDKGLVLPPKVASVQVIIVPVPYKD-ADTQGIFDACSTTLDTLSEAGIRAEVDSRD 355
           M HGDDKGLV PPKVA VQV+++ VP K  AD Q + DAC     TL  AGIRAE D RD
Sbjct: 89  MTHGDDKGLVFPPKVAPVQVVVIHVPIKGAADYQELCDACEAVESTLLGAGIRAEADIRD 148

Query: 356 NYSPGWKYSHWEMKGVPLRIEIGPKDLANNQVRAVRRDNSGKKDIPRAALVEQVKELLES 415
           NYS GWKY+  E+ GVPLRIE GP+DLAN+QVR V RDN  K D+ R  L+EQVK+LLE 
Sbjct: 149 NYSCGWKYADQELTGVPLRIETGPRDLANDQVRIVTRDNGAKMDVKRGDLIEQVKDLLEK 208

Query: 416 IQQSLFDAAKEKRDVCIQVINTWEEFTEALSQKKMILAPWCDEEEVEKDVKTRTKGEM-- 475
           IQ +L+D AK K + C Q + TW+EF EALSQKK+ILAPWCD+ EVEKDVK RT+G+   
Sbjct: 209 IQSNLYDVAKRKVEECTQKVETWDEFVEALSQKKLILAPWCDKVEVEKDVKRRTRGDETG 268

Query: 476 ---GAAKTLCSPFEQPALPEGTKCFASGKPAKKWSYWGRSY 511
              G AKTLC+P EQP L E T CFASGKPAKKWSYWGRSY
Sbjct: 269 GGGGGAKTLCTPLEQPELGEETLCFASGKPAKKWSYWGRSY 309

HSP 2 Score: 62.0 bits (149), Expect = 1.6e-09
Identity = 27/47 (57.45%), Postives = 37/47 (78.72%), Query Frame = 0


Query: 23 KETGLGLTNKKDDNFGEWYSEVVVSGEMIEYYD-ISGCYILRPWAMS 69
          KET LG+T KKD++FG+WYSE    GE++EYY+ + GCYIL+P  +S
Sbjct: 3  KETRLGITAKKDEDFGKWYSEACRFGELVEYYESVKGCYILKPSGIS 49

The following BLAST results are available for this feature:

BLAST of Csor.00g133400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M1R2	9.4e-257	83.57	Proline--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At3g62120 P...	[more]
Q8CGC7	9.3e-164	55.88	Bifunctional glutamate/proline--tRNA ligase OS=Mus musculus OX=10090 GN=Eprs1 PE...	[more]
O60155	3.5e-163	53.85	Putative proline--tRNA ligase C19C7.06 OS=Schizosaccharomyces pombe (strain 972 ...	[more]
P28668	4.6e-163	55.41	Bifunctional glutamate/proline--tRNA ligase OS=Drosophila melanogaster OX=7227 G...	[more]
P07814	6.1e-163	54.72	Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens OX=9606 GN=EPRS1 PE=...	[more]

BLAST of Csor.00g133400 vs. NCBI nr


Match Name	Identity	Description
XP_022955643.1	100.00	proline--tRNA ligase, cytoplasmic isoform X1 [Cucurbita moschata] >KAG6582053.1 ...	[more]
XP_023528664.1	99.80	proline--tRNA ligase, cytoplasmic isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022979761.1	99.02	proline--tRNA ligase, cytoplasmic-like [Cucurbita maxima]	[more]
XP_038907011.1	98.24	proline--tRNA ligase, cytoplasmic-like isoform X1 [Benincasa hispida] >XP_038907...	[more]
XP_023543704.1	97.85	proline--tRNA ligase, cytoplasmic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Csor.00g133400 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1GUJ5	100.00	Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111457586 PE=3 SV=1	[more]
A0A6J1IRP0	99.02	Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111479369 PE=3 SV=1	[more]
A0A6J1GYJ1	97.65	Prolyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111458443 PE=3 SV=1	[more]
A0A6J1JLY7	97.46	Prolyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111487085 PE=3 SV=1	[more]
A0A0A0KTZ1	97.26	Prolyl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_4G015750 PE=3 SV=1	[more]

BLAST of Csor.00g133400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62120.1	6.7e-258	83.57	Class II aaRS and biotin synthetases superfamily protein	[more]
AT3G62120.2	6.7e-258	83.57	Class II aaRS and biotin synthetases superfamily protein	[more]
AT5G52520.1	1.5e-116	41.39	Class II aaRS and biotin synthetases superfamily protein	[more]
AT5G10880.1	6.2e-78	65.16	tRNA synthetase-related / tRNA ligase-related	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002316	Proline-tRNA ligase, class IIa	PRINTS	PR01046	TRNASYNTHPRO	coord: 129..140 score: 49.38 coord: 159..167 score: 48.97 coord: 169..180 score: 42.59 coord: 88..106 score: 25.15
IPR016061	Proline-tRNA ligase, class II, C-terminal	SMART	SM00946	ProRS_C_1_2	coord: 437..510 e-value: 2.2E-28 score: 110.3
IPR016061	Proline-tRNA ligase, class II, C-terminal	PFAM	PF09180	ProRS-C_1	coord: 437..510 e-value: 3.2E-26 score: 91.4
IPR002314	Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)	PFAM	PF00587	tRNA-synt_2b	coord: 129..295 e-value: 7.1E-18 score: 65.2
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	TIGRFAM	TIGR00408	TIGR00408	coord: 31..510 e-value: 1.4E-179 score: 595.9
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	PANTHER	PTHR43382	PROLYL-TRNA SYNTHETASE	coord: 10..510
IPR004499	Proline-tRNA ligase, class IIa, archaeal-type	HAMAP	MF_01571	Pro_tRNA_synth_type3	coord: 30..510 score: 38.269661
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 30..307 e-value: 3.8E-110 score: 369.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR43382:SF4	PROLINE--TRNA LIGASE, CYTOPLASMIC-LIKE	coord: 10..510
None	No IPR available	CDD	cd00862	ProRS_anticodon_zinc	coord: 303..510 e-value: 1.93019E-92 score: 278.412
None	No IPR available	SUPERFAMILY	52954	Class II aaRS ABD-related	coord: 302..427
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 35..301
IPR004154	Anticodon-binding	PFAM	PF03129	HGTP_anticodon	coord: 314..410 e-value: 2.3E-18 score: 66.1
IPR017449	Prolyl-tRNA synthetase, class II	GENE3D	3.30.110.30		coord: 431..510 e-value: 2.0E-28 score: 100.5
IPR017449	Prolyl-tRNA synthetase, class II	SUPERFAMILY	64586	C-terminal domain of ProRS	coord: 432..510
IPR036621	Anticodon-binding domain superfamily	GENE3D	3.40.50.800		coord: 308..428 e-value: 2.0E-39 score: 136.1
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 69..307 score: 18.88479
IPR033721	Prolyl-tRNA synthetase, catalytic domain	CDD	cd00778	ProRS_core_arch_euk	coord: 36..297 e-value: 1.46685E-164 score: 464.376

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g133400.m01	Csor.00g133400.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006433	prolyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0017101	aminoacyl-tRNA synthetase multienzyme complex
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004827	proline-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding