Csor.00g107110 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g107110 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 5.6e-253
Identity = 442/760 (58.16%), Postives = 542/760 (71.32%), Query Frame = 0

Query: 10  SPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDAIFYSYKNH 69
           SP  A+KKSYIV LGSH+H  ++S+  L     SH   L SF+GS E AK+AIFYSYK H
Sbjct: 33  SPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRH 92

Query: 70  INGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPSSSPWRKAR 129
           INGFAAIL+E EA ++AKHP+V +V PNK ++LHTTHSW FM L KN V+  SS W KA 
Sbjct: 93  INGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAG 152

Query: 130 FGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLKLIGAKYFNQ 189
           +GED IIANLD+GVWPESKSF ++G  G VP+RWKG C       VPCN KLIGA+YFN+
Sbjct: 153 YGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRC----HKDVPCNRKLIGARYFNK 212

Query: 190 GALAY--LKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIGTAKGGS 249
           G LAY  L S    E       + RD+ GHGSHTLSTA G++V   +VFG G GTA GGS
Sbjct: 213 GYLAYTGLPSNASYE-------TCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGS 272

Query: 250 PKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDDIIAIGS 309
           PKARVAAYKVCWP  +   CFDAD+  A + AI DGVDVLS S+G    +Y  D IAIGS
Sbjct: 273 PKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGS 332

Query: 310 FHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSL 369
           FHA+K G+ VVCSAGNSGP   T SN+APW++TVGAS++DREFQA +EL NGQ F G+SL
Sbjct: 333 FHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSL 392

Query: 370 SNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSSRIDKGM 429
           S PL   K+Y LI+ A A        D++LCK  +LD  KVKGKILVCLRG ++R+DKGM
Sbjct: 393 SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGM 452

Query: 430 QALLADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMGYLIPPS 489
           QA  A A GM+LCND+ SG EI++D HVLP S I+Y DG+ +FSY+ STK+P GY+  P+
Sbjct: 453 QAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPT 512

Query: 490 SKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFDNRAVPY 549
           + +NTKP+P MA+FSSRGPN ++P I+KPD+TAPGVNIIAAF+ A  PT    DNR  P+
Sbjct: 513 ATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPF 572

Query: 550 ITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPIFAP 609
            T SGTSMSCPH+SG+VGLLK LHP WSPAAI+SAIMT++R  +N    M+D     F  
Sbjct: 573 NTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDES---FKK 632

Query: 610 ATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFT-DANFKCPVSGS 669
           A PF YGSGH++P  A  PGLVYDL+  DYL+FLCA GY    +++F  D  + C    +
Sbjct: 633 ANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGAN 692

Query: 670 ILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKFEKIGEE 729
           +L+FNYPSI V NLTGS+TV+R+LKNVG P  Y  R ++P GV+VSV+P  L F K GE 
Sbjct: 693 LLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEV 752

Query: 730 KGFELTMTG-AVAEGQIGYGTLIWTDDKHFVRSPIVVSSS 766
           K F++T+    V      +G L WTD  H+VRSPIVV  S
Sbjct: 753 KIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQLS 777

BLAST of Csor.00g107110 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 1.0e-230
Identity = 407/751 (54.19%), Postives = 529/751 (70.44%), Query Frame = 0

Query: 18  SYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 77
           SY+V  G+HSH  E++   + R  ++H+  LGSF GS E+A DAIFYSY  HINGFAA L
Sbjct: 31  SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90

Query: 78  EEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPSSSPWRKARFGEDVIIA 137
           + + A +++KHPEV +V PNKA +LHTT SW+F+ LE N  +PSSS WRKARFGED IIA
Sbjct: 91  DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150

Query: 138 NLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLKLIGAKYFNQGALAYLKS 197
           NLD+GVWPESKSF ++G+ G +PSRWKG C ++      CN KLIGA+YFN+G  A +  
Sbjct: 151 NLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGH 210

Query: 198 QNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIGTAKGGSPKARVAAYKV 257
            N   + P      RD  GHGSHTLSTA G +V  VS+FG G GTAKGGSP+ARVAAYKV
Sbjct: 211 LNSSFDSP------RDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKV 270

Query: 258 CWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDDIIAIGSFHALKKGIPV 317
           CWP      C+DADV  AFD AIHDG DV+S+S+G  P  +++D +AIGSFHA KK I V
Sbjct: 271 CWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVV 330

Query: 318 VCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSLSN-PLAGRKL 377
           VCSAGNSGP+ +T SN+APW +TVGAST+DREF + + LGNG+ + G SLS+  L   K 
Sbjct: 331 VCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKF 390

Query: 378 YPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSSRIDKGMQALLADAVG 437
           YP++    AKA  A A D+ LCK  +LD  K KGKILVCLRG + R++KG    L   +G
Sbjct: 391 YPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIG 450

Query: 438 MILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMGYLIPPSSKVNTKPSP 497
           M+L N  ++G +++AD HVLP + +   D  AV  YI  TK P+ ++ P  + +  KP+P
Sbjct: 451 MVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAP 510

Query: 498 TMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFDNRAVPYITMSGTSMS 557
            MA+FSS+GP+IV+P+I+KPD+TAPGV++IAA++GAVSPT E FD R + +  +SGTSMS
Sbjct: 511 VMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMS 570

Query: 558 CPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPIFAPATPFVYGSG 617
           CPH+SGI GLLK  +P WSPAAI+SAIMT+A + D+    + +  +     ATPF +G+G
Sbjct: 571 CPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAG 630

Query: 618 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFKCPVSG-SILNFNYPSI 677
           H++P  A++PGLVYDL   DYL FLC+ GY    + VF+  NF C     S++N NYPSI
Sbjct: 631 HVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSI 690

Query: 678 GVQNLTGS-VTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKFEKIGEEKGFELTMT 737
            V NLT S VTVSR +KNVG P +Y V+V  P+GV V+VKP  L F K+GE+K F++ + 
Sbjct: 691 TVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILV 750

Query: 738 ---GAVAEGQIGYGTLIWTDDKHFVRSPIVV 763
              G VA+G + +G L+W+D KH VRSPIVV
Sbjct: 751 KSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770

BLAST of Csor.00g107110 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 651.0 bits (1678), Expect = 1.7e-185
Identity = 371/784 (47.32%), Postives = 493/784 (62.88%), Query Frame = 0

Query: 3   FGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDAI 62
           F F +L++ V   KK YIV +G+HSHG   ++ DL+ A DSH+ LLGS  GS EKAK+AI
Sbjct: 16  FLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAI 75

Query: 63  FYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPSS 122
            YSY  HINGFAA+LEEEEA  +AK+P V +V  +K  +LHTT SWEF+ L +      +
Sbjct: 76  IYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG---QN 135

Query: 123 SPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGC--MDKTPDGV--PCN 182
           S W+K RFGE+ II N+D+GVWPES+SF ++G  G VPS+W+GG   ++K P  +   CN
Sbjct: 136 SAWQKGRFGENTIIGNIDTGVWPESQSFSDKG-YGTVPSKWRGGLCQINKLPGSMKNTCN 195

Query: 183 LKLIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGS 242
            KLIGA+Y+N+   A+        +L  ++++ RD+ GHG+HTLSTAGG++V    VF  
Sbjct: 196 RKLIGARYYNKAFEAH------NGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAV 255

Query: 243 GIGTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSP---P 302
           G GTAKGGSP+ARVAAYKVCW L +   C+ ADV  A D AI DGVDV+++S G      
Sbjct: 256 GNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVT 315

Query: 303 AE-YYDDIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPI 362
           AE  + D I+IG+FHA+ K I +V SAGN GP+  T +N+APW+ T+ ASTLDR+F + +
Sbjct: 316 AEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNL 375

Query: 363 ELGNGQRFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILV 422
            + N Q   G+SL   L   + + LI    AK   A  +D+ LC+  TLD +KV GKI++
Sbjct: 376 TI-NNQLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVL 435

Query: 423 CLRGGSSR-IDKGMQALLADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYI 482
           C R G  + + +G++AL A A GMIL N   +G  + A+ HV  T +      ++    +
Sbjct: 436 CTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGV 495

Query: 483 YST-----KNPM--GYLIPPS---SKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGV 542
            +T      +P+  G  I  S   +    KP+P MA+FSSRGPN + P I+KPD+TAPGV
Sbjct: 496 KTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGV 555

Query: 543 NIIAAFSGAVSPTGEPFDN-RAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSA 602
           NI+AA+S   S +    DN R   +  + GTSMSCPH SGI GLLK  HP WSPAAIKSA
Sbjct: 556 NILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSA 615

Query: 603 IMTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLC 662
           IMT+A   DNT   + D      A A  F YGSGH+RP  AI+PGLVYDLS  DYL FLC
Sbjct: 616 IMTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLC 675

Query: 663 ASGYTEKNLRVFT-DANFKCPVSGSILNFNYPSIGVQNL-TGSVTVSRRLKNVGMPGVYR 722
           ASGY ++ +     +  F C  S S+ + NYPSI + NL    VT++R + NVG P  Y 
Sbjct: 676 ASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYT 735

Query: 723 VRVQQPEGVKVSVKPNVLKFEKIGEEKGFELTM--TGAVAEGQIGYGTLIWTDDKHFVRS 763
           V  + P G  ++V P  L F KIGE K F++ +  + A    +  +G L WTD KH VRS
Sbjct: 736 VSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRS 786

BLAST of Csor.00g107110 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 5.9e-162
Identity = 325/732 (44.40%), Postives = 449/732 (61.34%), Query Frame = 0

Query: 42  DSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKE 101
           D H     S L S   + + + Y+Y+N I+GF+  L +EEA  L   P V +VLP    E
Sbjct: 46  DLHSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYE 105

Query: 102 LHTTHSWEFMHLEKN--DVIPSSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGV 161
           LHTT +  F+ L+++  D+ P +  +       DV++  LD+GVWPESKS+ ++G  G +
Sbjct: 106 LHTTRTPLFLGLDEHTADLFPEAGSY------SDVVVGVLDTGVWPESKSYSDEGF-GPI 165

Query: 162 PSRWKGGCMDKTP-DGVPCNLKLIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHG 221
           PS WKGGC   T      CN KLIGA++F +G   Y  +   ++E      S RD  GHG
Sbjct: 166 PSSWKGGCEAGTNFTASLCNRKLIGARFFARG---YESTMGPIDE-SKESRSPRDDDGHG 225

Query: 222 SHTLSTAGGSYVSNVSVFGSGIGTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDH 281
           +HT STA GS V   S+ G   GTA+G +P+ARVA YKVCW    +GGCF +D+  A D 
Sbjct: 226 THTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDK 285

Query: 282 AIHDGVDVLSLSIGSPPAEYYDDIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWI 341
           AI D V+VLS+S+G   ++YY D +AIG+F A+++GI V CSAGN+GPS ++ SN+APWI
Sbjct: 286 AIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWI 345

Query: 342 LTVGASTLDREFQAPIELGNGQRFTGSSL-SNPLAGRKLYPLITGAQAKATTAFAQDSIL 401
            TVGA TLDR+F A   LGNG+ FTG SL        KL P I    A   T    +  L
Sbjct: 346 TTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNAT----NGNL 405

Query: 402 CKPETLDHSKVKGKILVCLRGGSSRIDKGMQALLADAVGMILCNDRLSGFEIMADLHVLP 461
           C   TL   KVKGKI++C RG ++R+ KG     A  VGMIL N   +G E++AD H+LP
Sbjct: 406 CMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLP 465

Query: 462 TSHINYNDGQAVFSYIYSTKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPD 521
            + +    G  +  Y+ +  NP   +    + V  KPSP +AAFSSRGPN ++P I+KPD
Sbjct: 466 ATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPD 525

Query: 522 MTAPGVNIIAAFSGAVSPTGEPFDNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPA 581
           + APGVNI+AA++GA  PTG   D+R V +  +SGTSMSCPHVSG+  LLK++HPEWSPA
Sbjct: 526 LIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPA 585

Query: 582 AIKSAIMTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDY 641
           AI+SA+MT+A  +      +LD  +    P+TPF +G+GH+ PT A +PGL+YDL+  DY
Sbjct: 586 AIRSALMTTAYKTYKDGKPLLDIATG--KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDY 645

Query: 642 LEFLCASGYTEKNLRVFTDANFKCPVSG--SILNFNYPSIGVQ-NLTGSVTVSRRLKNVG 701
           L FLCA  YT   +R  +  N+ C  S   S+ + NYPS  V  +  G+   +R + +VG
Sbjct: 646 LGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVG 705

Query: 702 MPGVYRVRV-QQPEGVKVSVKPNVLKFEKIGEEKGFELTMT--GAVAEGQIGYGTLIWTD 761
             G Y V+V  +  GVK+SV+P VL F++  E+K + +T T   +   G   +G++ W+D
Sbjct: 706 GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSD 755

Query: 762 DKHFVRSPIVVS 764
            KH V SP+ +S
Sbjct: 766 GKHVVGSPVAIS 755

BLAST of Csor.00g107110 vs. ExPASy Swiss-Prot
Match: A9JQS7 (Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 8.8e-158
Identity = 334/757 (44.12%), Postives = 457/757 (60.37%), Query Frame = 0

Query: 17  KSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAI 76
           K YIV +G  SH       + +  V ++H++L S  GS   AK A  + Y     GF+A+
Sbjct: 26  KHYIVYMGDRSH------PNSESVVRANHEILASVTGSLNDAKAAAIHHYSRSFQGFSAM 85

Query: 77  LEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPSSSPWRKARFGEDVII 136
           +  E+A KLA H  V +V  +K  +LHTTHSW+F+ L   D +  ++P        +VI+
Sbjct: 86  ITPEQAKKLADHNSVVSVFESKMNKLHTTHSWDFLGL---DTVYKNNP-SALDSASNVIV 145

Query: 137 ANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDG---VPCNLKLIGAKYFNQGALA 196
             +DSGVWPES+SF + G+ G VP ++KG C+  T D      CN K+IGA+++++G  A
Sbjct: 146 GVIDSGVWPESESFNDYGL-GPVPEKFKGECV--TGDNFTLANCNKKIIGARFYSKGLEA 205

Query: 197 YL-KSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIGTAKGGSPKARV 256
            +   +N+++   +   S RD  GHG+HT ST  GS VSNVS+FG   GTA+GG+P AR+
Sbjct: 206 EIGPLENIVDS--IFFRSPRDSDGHGTHTASTIAGSIVSNVSLFGMAKGTARGGAPSARL 265

Query: 257 AAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAE--YYDDIIAIGSFHA 316
           + YK CW     G C DADV  A D AIHDGVD+LSLS+G  P +  Y+++ I++G+FHA
Sbjct: 266 SIYKACW----FGFCSDADVFAAMDDAIHDGVDILSLSLGPDPPQPLYFENAISVGAFHA 325

Query: 317 LKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSLSNP 376
            +KGI V  SAGNS     TA N+APWI TV AST+DREF++ I LGN +   G SL NP
Sbjct: 326 FQKGILVSASAGNS-VFPRTACNVAPWIFTVAASTVDREFRSDIYLGNSKVLKGLSL-NP 385

Query: 377 LAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCL--RGGSSRIDKGMQ 436
           +     Y LI G+ A A    A ++  CK  TLD + +KGKI++C   +   +R +K + 
Sbjct: 386 IKMEGSYGLIYGSAAAAAGDAALNASFCKEHTLDPTLIKGKIVICTVEKFTDNRREKAII 445

Query: 437 ALLADAVGMILC--NDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMGYLIPP 496
                 VGMIL   N R  GF+      V+P++ I  +  + + +Y+ + KNP   + P 
Sbjct: 446 IKQGGGVGMILIDHNARDVGFQF-----VIPSTMIGQDAVEELQAYMKTEKNPTATIFPT 505

Query: 497 SSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFDNRAVP 556
            + V TKP+P  AAFSS GPNI++P+IIKPD+T PGVNI+AA+S     T    + ++V 
Sbjct: 506 LTLVGTKPAPESAAFSSVGPNIITPDIIKPDITGPGVNILAAWSPVA--TEATVEQKSVN 565

Query: 557 YITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPIFA 616
           Y  +SGTSMSCPH+S I  ++K+ HP WSPAAI SAIMTSA V DNT +L+  G  P   
Sbjct: 566 YNIISGTSMSCPHISAISAIIKSHHPSWSPAAIMSAIMTSATVMDNTHSLI--GRDPNGT 625

Query: 617 PATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFKCPVSGS 676
            ATPF YGSGH+ P  +++PGLVYD S  D L FLC++G +   L+  T    +C  S +
Sbjct: 626 QATPFDYGSGHVNPVASLNPGLVYDFSSQDVLNFLCSNGASPAQLKNLTGELTQCQKSPT 685

Query: 677 I-LNFNYPSIGVQNLTGSVTVSRRLKNVGM-PGVYRVRVQQPEGVKVSVKPNVLKFEKIG 736
              NFNYPSIGV NL GS++V R +   G  P  Y   V++P GV V V P  LKF K G
Sbjct: 686 ASYNFNYPSIGVSNLNGSLSVYRTVTYYGQEPTEYFASVERPSGVIVRVTPAKLKFWKAG 745

Query: 737 EEKGFELTMTG-AVAEGQIGYGTLIWTDDKHFVRSPI 761
           E+  F +  T    + G   +G L W + K  VRSPI
Sbjct: 746 EKITFRIDFTPFKNSNGNFVFGALTWNNGKQRVRSPI 752

BLAST of Csor.00g107110 vs. NCBI nr
Match: KAG6583681.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1541 bits (3991), Expect = 0.0
Identity = 767/767 (100.00%), Postives = 767/767 (100.00%), Query Frame = 0

Query: 1   MLFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKD 60
           MLFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKD
Sbjct: 1   MLFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKD 60

Query: 61  AIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIP 120
           AIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIP
Sbjct: 61  AIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIP 120

Query: 121 SSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLK 180
           SSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLK
Sbjct: 121 SSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLK 180

Query: 181 LIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGI 240
           LIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGI
Sbjct: 181 LIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGI 240

Query: 241 GTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYD 300
           GTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYD
Sbjct: 241 GTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYD 300

Query: 301 DIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQ 360
           DIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQ
Sbjct: 301 DIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQ 360

Query: 361 RFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGS 420
           RFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGS
Sbjct: 361 RFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGS 420

Query: 421 SRIDKGMQALLADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPM 480
           SRIDKGMQALLADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPM
Sbjct: 421 SRIDKGMQALLADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPM 480

Query: 481 GYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPF 540
           GYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPF
Sbjct: 481 GYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPF 540

Query: 541 DNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDG 600
           DNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDG
Sbjct: 541 DNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDG 600

Query: 601 GSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFK 660
           GSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFK
Sbjct: 601 GSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFK 660

Query: 661 CPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKF 720
           CPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKF
Sbjct: 661 CPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKF 720

Query: 721 EKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 767
           EKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF
Sbjct: 721 EKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 767

BLAST of Csor.00g107110 vs. NCBI nr
Match: XP_022927097.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 1528 bits (3955), Expect = 0.0
Identity = 761/767 (99.22%), Postives = 762/767 (99.35%), Query Frame = 0

Query: 1   MLFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKD 60
           MLFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSS KAKD
Sbjct: 10  MLFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSGKAKD 69

Query: 61  AIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIP 120
           AIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWE MHLEKNDVIP
Sbjct: 70  AIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWESMHLEKNDVIP 129

Query: 121 SSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLK 180
           SSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCN K
Sbjct: 130 SSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNRK 189

Query: 181 LIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGI 240
           LIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGI
Sbjct: 190 LIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGI 249

Query: 241 GTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYD 300
           GTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYD
Sbjct: 250 GTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYD 309

Query: 301 DIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQ 360
           DIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQ
Sbjct: 310 DIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQ 369

Query: 361 RFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGS 420
           RFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGS
Sbjct: 370 RFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGS 429

Query: 421 SRIDKGMQALLADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPM 480
           SRIDKGMQALLA AVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPM
Sbjct: 430 SRIDKGMQALLAGAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPM 489

Query: 481 GYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPF 540
           GYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPD+TAPGVNIIAAFSGAVSPTGEPF
Sbjct: 490 GYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVNIIAAFSGAVSPTGEPF 549

Query: 541 DNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDG 600
           DNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDG
Sbjct: 550 DNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDG 609

Query: 601 GSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFK 660
           GSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTD NFK
Sbjct: 610 GSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDGNFK 669

Query: 661 CPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKF 720
           CPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKF
Sbjct: 670 CPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKF 729

Query: 721 EKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 767
           EKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF
Sbjct: 730 EKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 776

BLAST of Csor.00g107110 vs. NCBI nr
Match: XP_023520266.1 (subtilisin-like protease SBT5.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1374 bits (3556), Expect = 0.0
Identity = 675/766 (88.12%), Postives = 713/766 (93.08%), Query Frame = 0

Query: 2   LFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDA 61
           LFGFFWLISP IA+KKSYIVLLGSH+HGLEVSAVDLQRAVDSHHKLLGSFLGSS KAKDA
Sbjct: 10  LFGFFWLISPAIAIKKSYIVLLGSHAHGLEVSAVDLQRAVDSHHKLLGSFLGSSGKAKDA 69

Query: 62  IFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPS 121
           IFYSYKNHINGFAAIL+EEEATKLAKHPEVAAVLPNKAKELHTT SWEFMHLEKN V P 
Sbjct: 70  IFYSYKNHINGFAAILDEEEATKLAKHPEVAAVLPNKAKELHTTRSWEFMHLEKNGVSPP 129

Query: 122 SSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLKL 181
           SSPWRKA+ G +VIIANLD+GVWPESKSFGE GIVGGVPSRWKGGC DKTPD VPCN KL
Sbjct: 130 SSPWRKAKSGRNVIIANLDTGVWPESKSFGEHGIVGGVPSRWKGGCTDKTPDAVPCNRKL 189

Query: 182 IGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIG 241
           IGAKYFN G +AYLKS+NL +EL  IVNSTRDY+GHGSHTLSTAGGSYVS VSVFGSGIG
Sbjct: 190 IGAKYFNHGVIAYLKSENLTKELSFIVNSTRDYEGHGSHTLSTAGGSYVSGVSVFGSGIG 249

Query: 242 TAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDD 301
           TAKGGSPKA VAAYKVCWPL + GGCFDAD+A+AFDHAIHDGVDVLSLS+GSPPAEYYDD
Sbjct: 250 TAKGGSPKAHVAAYKVCWPLHDTGGCFDADIAKAFDHAIHDGVDVLSLSLGSPPAEYYDD 309

Query: 302 IIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQR 361
           IIAI SFHALKKGIPVVCSAGN GPSMAT SN APWILTVGASTLDREFQAPIEL NG+ 
Sbjct: 310 IIAIASFHALKKGIPVVCSAGNDGPSMATVSNTAPWILTVGASTLDREFQAPIELRNGKH 369

Query: 362 FTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSS 421
           F GSSLS PL+GRKLYPLITGAQAKATTA   D++LCKP+TLDHSKVKGKILVCLRG SS
Sbjct: 370 FKGSSLSGPLSGRKLYPLITGAQAKATTASTDDAMLCKPKTLDHSKVKGKILVCLRGDSS 429

Query: 422 RIDKGMQALLADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMG 481
           R+DKG+QALLA AVGMILCNDRLSGFEI+AD HVLP SHI+YNDGQ V SYI STKNPMG
Sbjct: 430 RVDKGVQALLAGAVGMILCNDRLSGFEIIADPHVLPASHISYNDGQVVSSYINSTKNPMG 489

Query: 482 YLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFD 541
           +LIPP SKVNTKPSPTMAAFSSRGPN+VSPEIIKPD+TAPGVNIIAAFSGAVSPTGEPFD
Sbjct: 490 HLIPPLSKVNTKPSPTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFD 549

Query: 542 NRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGG 601
           NR VPYITMSGTSMSCPHVSGIVGLLKALHP+WSPAAIKSAIMTSAR+ DNTMN+MLDGG
Sbjct: 550 NRTVPYITMSGTSMSCPHVSGIVGLLKALHPKWSPAAIKSAIMTSARIRDNTMNIMLDGG 609

Query: 602 SPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFKC 661
           SPIFAPATPF++GSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGY EKNLRVF D NFKC
Sbjct: 610 SPIFAPATPFIFGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNLRVFADGNFKC 669

Query: 662 PVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKFE 721
           P+S SILNFNYPSIGVQNLTG+VT++RRLKNVG PGVYRVRVQQPEGVKVSVKP+VLKF+
Sbjct: 670 PISSSILNFNYPSIGVQNLTGNVTLTRRLKNVGRPGVYRVRVQQPEGVKVSVKPSVLKFK 729

Query: 722 KIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 767
           KIGEEK FELTM GA+A  QIGYGTLIWTD KHFVRSPIVVSS FF
Sbjct: 730 KIGEEKRFELTMIGAMANSQIGYGTLIWTDGKHFVRSPIVVSSGFF 775

BLAST of Csor.00g107110 vs. NCBI nr
Match: KAG6583680.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1360 bits (3521), Expect = 0.0
Identity = 671/766 (87.60%), Postives = 711/766 (92.82%), Query Frame = 0

Query: 2   LFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDA 61
           L GFF LISP IAVKKSYIVLLGSH+HGLEVSAVDLQR VDSHHKLLGSFLGSS+KAKDA
Sbjct: 10  LLGFFGLISPTIAVKKSYIVLLGSHAHGLEVSAVDLQRVVDSHHKLLGSFLGSSKKAKDA 69

Query: 62  IFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPS 121
           IFYSYKNHINGFAAIL+EEEATKLAKHP++AAVLPNKAKELHTT SWEFMHLEKN V P 
Sbjct: 70  IFYSYKNHINGFAAILDEEEATKLAKHPDIAAVLPNKAKELHTTRSWEFMHLEKNGVSPP 129

Query: 122 SSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLKL 181
           SSPWRKAR G++VIIANLD+GVWPESKSFGE GIVGGVPS+WKGGCMDKTPD VPCN KL
Sbjct: 130 SSPWRKARSGQNVIIANLDTGVWPESKSFGEHGIVGGVPSKWKGGCMDKTPDAVPCNKKL 189

Query: 182 IGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIG 241
           IGAKYFN G +AYLKS+NL +EL  IVNSTRDY+GHGSHTLSTAGGSYVS VSVFGSGIG
Sbjct: 190 IGAKYFNHGVIAYLKSENLTKELSFIVNSTRDYEGHGSHTLSTAGGSYVSGVSVFGSGIG 249

Query: 242 TAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDD 301
           TAKGGSPKA VAAYKVCWPL + GGCFDAD+A+AFDHAIHDGVDVLS+S+GSPPAEYY+D
Sbjct: 250 TAKGGSPKAHVAAYKVCWPLHDTGGCFDADIAKAFDHAIHDGVDVLSVSLGSPPAEYYND 309

Query: 302 IIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQR 361
           IIAI SFHALKKGI VVCSAGN GPSMAT SN APWILTVGASTLDREFQAPIEL NG+ 
Sbjct: 310 IIAIASFHALKKGILVVCSAGNDGPSMATVSNTAPWILTVGASTLDREFQAPIELRNGKH 369

Query: 362 FTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSS 421
           F GSSLS PL+GRKLYPLITGAQAKATTA   D++LCKP+TLDHSKVKGKILVCLRG SS
Sbjct: 370 FKGSSLSGPLSGRKLYPLITGAQAKATTASTDDAMLCKPKTLDHSKVKGKILVCLRGDSS 429

Query: 422 RIDKGMQALLADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMG 481
           R+DKG+QALLA AVGMILCNDRLSGFEI+AD HVLP SHI+YNDGQAV SYI STKNPMG
Sbjct: 430 RVDKGVQALLAGAVGMILCNDRLSGFEIIADPHVLPASHISYNDGQAVSSYINSTKNPMG 489

Query: 482 YLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFD 541
           +LIPP SKVNTKPSPTMAAFSSRGPN+VSPEIIKPD+TAPGVNIIAAFSGAVSPTGEPFD
Sbjct: 490 HLIPPLSKVNTKPSPTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFD 549

Query: 542 NRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGG 601
           NR VPYITMSGTSMSCPHVSGIVGLLKALHP+WSPAAIKSAIMTSAR+ DNTMN+MLDGG
Sbjct: 550 NRTVPYITMSGTSMSCPHVSGIVGLLKALHPKWSPAAIKSAIMTSARIRDNTMNIMLDGG 609

Query: 602 SPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFKC 661
           SPIFA AT F++GSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGY EKNLRVF D NFKC
Sbjct: 610 SPIFASATAFIFGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNLRVFVDGNFKC 669

Query: 662 PVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKFE 721
           PVS SILNFNYPSIGVQNLTG+VT++RRLKNVG PGVYRVRVQQPEGVKVSVKP+VLKFE
Sbjct: 670 PVSSSILNFNYPSIGVQNLTGNVTLTRRLKNVGRPGVYRVRVQQPEGVKVSVKPSVLKFE 729

Query: 722 KIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 767
           KIGEEK FELTMTG VA GQIGYGTLIWTD KH VRSPIVVSS FF
Sbjct: 730 KIGEEKRFELTMTGVVANGQIGYGTLIWTDGKHLVRSPIVVSSGFF 775

BLAST of Csor.00g107110 vs. NCBI nr
Match: XP_022927102.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 1353 bits (3502), Expect = 0.0
Identity = 670/752 (89.10%), Postives = 698/752 (92.82%), Query Frame = 0

Query: 16   KKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAA 75
            K+SYIVLLGSHSHGLEVSAVDLQR VDSHHKLLGS LGSSEKAKDAIF+SYKNHINGFAA
Sbjct: 251  KESYIVLLGSHSHGLEVSAVDLQRVVDSHHKLLGSLLGSSEKAKDAIFHSYKNHINGFAA 310

Query: 76   ILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPSSSPWRKARFGEDVI 135
            ILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKN V+   SPWRKARFG DVI
Sbjct: 311  ILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGVVLPFSPWRKARFGRDVI 370

Query: 136  IANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLKLIGAKYFNQGALAYL 195
            IANLD+GVWPESKSFGEQGIVGGVPSRWKGGCMDKTPD VPCN KLIGAKYFNQG +AYL
Sbjct: 371  IANLDTGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDAVPCNRKLIGAKYFNQGLIAYL 430

Query: 196  KSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIGTAKGGSPKARVAAY 255
            KS NL +EL LIVNSTRDY GHG+HTLSTAGGSYVS VSVFGSGIGTAKGGSPKARVAAY
Sbjct: 431  KSHNLTDELSLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAY 490

Query: 256  KVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDDIIAIGSFHALKKGI 315
            KVCWP  N GGC+D DV + FD AIHDGVDVLSLSIGSPPAEYYDD IAI SF A+KKGI
Sbjct: 491  KVCWPFHNSGGCYDIDVVDGFDQAIHDGVDVLSLSIGSPPAEYYDDTIAIASFLAIKKGI 550

Query: 316  PVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSLSNPLAGRK 375
            PVVCSAGNSGPSMA+ASNIAPWILTVGASTLDREFQAPIELGNGQRF GSSLS PLA RK
Sbjct: 551  PVVCSAGNSGPSMASASNIAPWILTVGASTLDREFQAPIELGNGQRFWGSSLSRPLARRK 610

Query: 376  LYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSSRIDKGMQALLADAV 435
            LYPLITGAQAKATTA A D++LCKP+TLDHSKV GKILVCL GGSSRIDKGMQA+LA AV
Sbjct: 611  LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAV 670

Query: 436  GMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMGYLIPPSSKVNTKPS 495
            GMILCNDR SGF+I+ADLHVLP SHINYNDGQAVFSYI S KNPMGYLIPPSSKVNTKPS
Sbjct: 671  GMILCNDRFSGFKIIADLHVLPASHINYNDGQAVFSYINSWKNPMGYLIPPSSKVNTKPS 730

Query: 496  PTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFDNRAVPYITMSGTSM 555
            PTMAAFSSRGPN+VSPEIIKPD+TAPGVNIIAAFSGAVSPT EPFDNR VPYITMSGTSM
Sbjct: 731  PTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGAVSPTREPFDNRTVPYITMSGTSM 790

Query: 556  SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPIFAPATPFVYGS 615
            SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA +SDNTMNL+LDGGSP+FAPATPF+YGS
Sbjct: 791  SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSAIISDNTMNLILDGGSPMFAPATPFIYGS 850

Query: 616  GHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFKCPVSGSILNFNYPSI 675
            GHI PTGAIDPGLVYDLSPNDYLEFLCASGY EKN+RVF D NFKCP+S SI NFNYPSI
Sbjct: 851  GHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPISCSIFNFNYPSI 910

Query: 676  GVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKFEKIGEEKGFELTMTG 735
            GVQNLTGSVT+ RRLKNVG PGVYRVRV+QPEGVKVSVKP VLKF KIGEEK FELTMTG
Sbjct: 911  GVQNLTGSVTLKRRLKNVGRPGVYRVRVRQPEGVKVSVKPRVLKFWKIGEEKRFELTMTG 970

Query: 736  AVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 767
            A+AEGQIGYGTLIWTD KHFVRSPIVVSS  F
Sbjct: 971  AMAEGQIGYGTLIWTDGKHFVRSPIVVSSGLF 1002

BLAST of Csor.00g107110 vs. ExPASy TrEMBL
Match: A0A6J1EG66 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434032 PE=3 SV=1)

HSP 1 Score: 1528 bits (3955), Expect = 0.0
Identity = 761/767 (99.22%), Postives = 762/767 (99.35%), Query Frame = 0

Query: 1   MLFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKD 60
           MLFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSS KAKD
Sbjct: 10  MLFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSGKAKD 69

Query: 61  AIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIP 120
           AIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWE MHLEKNDVIP
Sbjct: 70  AIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWESMHLEKNDVIP 129

Query: 121 SSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLK 180
           SSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCN K
Sbjct: 130 SSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNRK 189

Query: 181 LIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGI 240
           LIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGI
Sbjct: 190 LIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGI 249

Query: 241 GTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYD 300
           GTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYD
Sbjct: 250 GTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYD 309

Query: 301 DIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQ 360
           DIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQ
Sbjct: 310 DIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQ 369

Query: 361 RFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGS 420
           RFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGS
Sbjct: 370 RFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGS 429

Query: 421 SRIDKGMQALLADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPM 480
           SRIDKGMQALLA AVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPM
Sbjct: 430 SRIDKGMQALLAGAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPM 489

Query: 481 GYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPF 540
           GYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPD+TAPGVNIIAAFSGAVSPTGEPF
Sbjct: 490 GYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVNIIAAFSGAVSPTGEPF 549

Query: 541 DNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDG 600
           DNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDG
Sbjct: 550 DNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDG 609

Query: 601 GSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFK 660
           GSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTD NFK
Sbjct: 610 GSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDGNFK 669

Query: 661 CPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKF 720
           CPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKF
Sbjct: 670 CPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKF 729

Query: 721 EKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 767
           EKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF
Sbjct: 730 EKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 776

BLAST of Csor.00g107110 vs. ExPASy TrEMBL
Match: A0A6J1EG71 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434037 PE=3 SV=1)

HSP 1 Score: 1353 bits (3502), Expect = 0.0
Identity = 670/752 (89.10%), Postives = 698/752 (92.82%), Query Frame = 0

Query: 16   KKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAA 75
            K+SYIVLLGSHSHGLEVSAVDLQR VDSHHKLLGS LGSSEKAKDAIF+SYKNHINGFAA
Sbjct: 251  KESYIVLLGSHSHGLEVSAVDLQRVVDSHHKLLGSLLGSSEKAKDAIFHSYKNHINGFAA 310

Query: 76   ILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPSSSPWRKARFGEDVI 135
            ILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKN V+   SPWRKARFG DVI
Sbjct: 311  ILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGVVLPFSPWRKARFGRDVI 370

Query: 136  IANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLKLIGAKYFNQGALAYL 195
            IANLD+GVWPESKSFGEQGIVGGVPSRWKGGCMDKTPD VPCN KLIGAKYFNQG +AYL
Sbjct: 371  IANLDTGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDAVPCNRKLIGAKYFNQGLIAYL 430

Query: 196  KSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIGTAKGGSPKARVAAY 255
            KS NL +EL LIVNSTRDY GHG+HTLSTAGGSYVS VSVFGSGIGTAKGGSPKARVAAY
Sbjct: 431  KSHNLTDELSLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAY 490

Query: 256  KVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDDIIAIGSFHALKKGI 315
            KVCWP  N GGC+D DV + FD AIHDGVDVLSLSIGSPPAEYYDD IAI SF A+KKGI
Sbjct: 491  KVCWPFHNSGGCYDIDVVDGFDQAIHDGVDVLSLSIGSPPAEYYDDTIAIASFLAIKKGI 550

Query: 316  PVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSLSNPLAGRK 375
            PVVCSAGNSGPSMA+ASNIAPWILTVGASTLDREFQAPIELGNGQRF GSSLS PLA RK
Sbjct: 551  PVVCSAGNSGPSMASASNIAPWILTVGASTLDREFQAPIELGNGQRFWGSSLSRPLARRK 610

Query: 376  LYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSSRIDKGMQALLADAV 435
            LYPLITGAQAKATTA A D++LCKP+TLDHSKV GKILVCL GGSSRIDKGMQA+LA AV
Sbjct: 611  LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAV 670

Query: 436  GMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMGYLIPPSSKVNTKPS 495
            GMILCNDR SGF+I+ADLHVLP SHINYNDGQAVFSYI S KNPMGYLIPPSSKVNTKPS
Sbjct: 671  GMILCNDRFSGFKIIADLHVLPASHINYNDGQAVFSYINSWKNPMGYLIPPSSKVNTKPS 730

Query: 496  PTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFDNRAVPYITMSGTSM 555
            PTMAAFSSRGPN+VSPEIIKPD+TAPGVNIIAAFSGAVSPT EPFDNR VPYITMSGTSM
Sbjct: 731  PTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGAVSPTREPFDNRTVPYITMSGTSM 790

Query: 556  SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPIFAPATPFVYGS 615
            SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA +SDNTMNL+LDGGSP+FAPATPF+YGS
Sbjct: 791  SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSAIISDNTMNLILDGGSPMFAPATPFIYGS 850

Query: 616  GHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFKCPVSGSILNFNYPSI 675
            GHI PTGAIDPGLVYDLSPNDYLEFLCASGY EKN+RVF D NFKCP+S SI NFNYPSI
Sbjct: 851  GHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPISCSIFNFNYPSI 910

Query: 676  GVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKFEKIGEEKGFELTMTG 735
            GVQNLTGSVT+ RRLKNVG PGVYRVRV+QPEGVKVSVKP VLKF KIGEEK FELTMTG
Sbjct: 911  GVQNLTGSVTLKRRLKNVGRPGVYRVRVRQPEGVKVSVKPRVLKFWKIGEEKRFELTMTG 970

Query: 736  AVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 767
            A+AEGQIGYGTLIWTD KHFVRSPIVVSS  F
Sbjct: 971  AMAEGQIGYGTLIWTDGKHFVRSPIVVSSGLF 1002

BLAST of Csor.00g107110 vs. ExPASy TrEMBL
Match: A0A6J1EGQ8 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434031 PE=3 SV=1)

HSP 1 Score: 1344 bits (3479), Expect = 0.0
Identity = 659/752 (87.63%), Postives = 701/752 (93.22%), Query Frame = 0

Query: 16  KKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAA 75
           ++SYIVLLGSH+HGLEVSAVDLQR VDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAA
Sbjct: 2   EESYIVLLGSHAHGLEVSAVDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAA 61

Query: 76  ILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPSSSPWRKARFGEDVI 135
           IL+EEEATKLAKHPE+AAVLPNKAKELHTT SWEFMHLEKN V P SSPWRKAR G++VI
Sbjct: 62  ILDEEEATKLAKHPEIAAVLPNKAKELHTTRSWEFMHLEKNGVSPPSSPWRKARSGQNVI 121

Query: 136 IANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLKLIGAKYFNQGALAYL 195
           IANLD+GVWPESKSFGE GIVGGVPS+WKGGCMDKTPD VPCN KLIGAKYFN G +AYL
Sbjct: 122 IANLDTGVWPESKSFGEHGIVGGVPSKWKGGCMDKTPDAVPCNKKLIGAKYFNHGVIAYL 181

Query: 196 KSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIGTAKGGSPKARVAAY 255
           KS+NL +EL  IVNSTRDY+GHGSHTLSTAGGSYVS VSVFGSGIGTAKGGSPKA VAAY
Sbjct: 182 KSENLTKELSFIVNSTRDYEGHGSHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKAHVAAY 241

Query: 256 KVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDDIIAIGSFHALKKGI 315
           KVCWPL + GGCFDAD+A+AFDHAIHDGVDVLS+S+GSPPAEYY+DIIAI SFHALKKGI
Sbjct: 242 KVCWPLHDTGGCFDADIAKAFDHAIHDGVDVLSVSLGSPPAEYYNDIIAIASFHALKKGI 301

Query: 316 PVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSLSNPLAGRK 375
           PVVCSAGN GPSMAT SN APWILTVGASTLDREFQAPIEL NG+ F GSSLS PL+GRK
Sbjct: 302 PVVCSAGNDGPSMATVSNTAPWILTVGASTLDREFQAPIELRNGKHFKGSSLSGPLSGRK 361

Query: 376 LYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSSRIDKGMQALLADAV 435
           LYPLITGAQAKATTA   D++LCKP+TLDHSKVKGKILVCLRG SSR+DKG+QALLA AV
Sbjct: 362 LYPLITGAQAKATTASTDDAMLCKPKTLDHSKVKGKILVCLRGDSSRVDKGVQALLAGAV 421

Query: 436 GMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMGYLIPPSSKVNTKPS 495
           GMILCNDRLSGFEI+AD HVLP SHI+YNDGQAV SYI STKNPMG+LIPP SKVNTKPS
Sbjct: 422 GMILCNDRLSGFEIIADPHVLPASHISYNDGQAVSSYINSTKNPMGHLIPPLSKVNTKPS 481

Query: 496 PTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFDNRAVPYITMSGTSM 555
           PTMAAFSSRGPN+VSPEIIKPD+TAPGVNIIAAFSG+VSPTGEPFDNR VPYITMSGTSM
Sbjct: 482 PTMAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGSVSPTGEPFDNRTVPYITMSGTSM 541

Query: 556 SCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPIFAPATPFVYGS 615
           SCPHVSGIVGL+KALHP+WSPAAIKSAIMTSAR+ DNTMN+MLDGGSPIFA AT F++GS
Sbjct: 542 SCPHVSGIVGLVKALHPKWSPAAIKSAIMTSARIRDNTMNIMLDGGSPIFASATAFIFGS 601

Query: 616 GHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFKCPVSGSILNFNYPSI 675
           GHIRPTGAIDPGLVYDLSPNDYLEFLCASGY EKNLRVF D NFKCPVS SILNFNYPSI
Sbjct: 602 GHIRPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNLRVFADGNFKCPVSSSILNFNYPSI 661

Query: 676 GVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKFEKIGEEKGFELTMTG 735
           GVQNLTG+VT++RRLKNVG PGVYRVRVQQPEGVKVSVKP+VLKFEKIGEEK FELTMTG
Sbjct: 662 GVQNLTGNVTLTRRLKNVGRPGVYRVRVQQPEGVKVSVKPSVLKFEKIGEEKRFELTMTG 721

Query: 736 AVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 767
            VA GQIGYGTLIWTD KH VRSPIVVSS FF
Sbjct: 722 VVANGQIGYGTLIWTDGKHLVRSPIVVSSGFF 753

BLAST of Csor.00g107110 vs. ExPASy TrEMBL
Match: A0A6J1EK27 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434038 PE=3 SV=1)

HSP 1 Score: 1343 bits (3477), Expect = 0.0
Identity = 660/753 (87.65%), Postives = 697/753 (92.56%), Query Frame = 0

Query: 15  VKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA 74
           + +SYIVLLGSHSHGLEVSA+D+QR VDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA
Sbjct: 16  ILQSYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFA 75

Query: 75  AILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPSSSPWRKARFGEDV 134
           AIL+EEEAT LAKHPEVAAVLPNKAKELHTTHSWEFMHLEKN  I   SPWRKARFG+DV
Sbjct: 76  AILDEEEATNLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDV 135

Query: 135 IIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLKLIGAKYFNQGALAY 194
           IIANLD+GVWPESKSFGEQGI GGVPSRWKGGCMDKTPD VPCN KLIGAKYFNQG +AY
Sbjct: 136 IIANLDTGVWPESKSFGEQGITGGVPSRWKGGCMDKTPDAVPCNKKLIGAKYFNQGLIAY 195

Query: 195 LKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIGTAKGGSPKARVAA 254
           LKS NL +ELPLIVNSTRDY GHG+HTLSTAGGSYVS VSVFGSGIGTAKGGSPKARVAA
Sbjct: 196 LKSHNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAA 255

Query: 255 YKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDDIIAIGSFHALKKG 314
           YKVCWP  N GGC+DAD+ + FD AIHDGVDVLSLSIGSPP EYYDD IAI SFHALKKG
Sbjct: 256 YKVCWPFRNGGGCYDADIFDGFDQAIHDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKG 315

Query: 315 IPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSLSNPLAGR 374
           IPVVCSAGNSGPSMATA+NIAPWILTVGASTLDREFQAPIELGNGQRF G SLS PLA R
Sbjct: 316 IPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARR 375

Query: 375 KLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSSRIDKGMQALLADA 434
           KLYPLITGAQAKATT  A D++LCKP+TLDHSKV GKILVCL GGSSRIDKGMQA+LA A
Sbjct: 376 KLYPLITGAQAKATTTSADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGA 435

Query: 435 VGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMGYLIPPSSKVNTKP 494
           VGMILCNDR SGF+I+ADLHVLP SHI+YNDGQA+ SYI S KNPMGYLIPPSSKVNTKP
Sbjct: 436 VGMILCNDRFSGFKIIADLHVLPASHISYNDGQAISSYINSRKNPMGYLIPPSSKVNTKP 495

Query: 495 SPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFDNRAVPYITMSGTS 554
           SPTMAAFSSRGPNIVSPEIIKPD+TAPGV+IIAAFSGAVSPTGEPFDNR VPYITMSGTS
Sbjct: 496 SPTMAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTS 555

Query: 555 MSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPIFAPATPFVYG 614
           MSCPHVSGIVGLLKALHP+WSPAAIKSAIMTSA +SDNTMNL+LDGGSPIFAPATPF+YG
Sbjct: 556 MSCPHVSGIVGLLKALHPQWSPAAIKSAIMTSATISDNTMNLILDGGSPIFAPATPFIYG 615

Query: 615 SGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFKCPVSGSILNFNYPS 674
           SGHI P GAIDPGLVYDLSPNDYLEFLCASGY EKN+RVF + N+KCPVSGSILNFNYPS
Sbjct: 616 SGHIHPIGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFAEENYKCPVSGSILNFNYPS 675

Query: 675 IGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKFEKIGEEKGFELTMT 734
           IGVQNLTG VT++RRLKNVG PGVYRVRV++P+GVKV VKP VLKF KIGEEK FELTMT
Sbjct: 676 IGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPKGVKVLVKPRVLKFRKIGEEKRFELTMT 735

Query: 735 GAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 767
           G VAEGQIGYGTLIWTD KHFV+SPIVVSS FF
Sbjct: 736 GVVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 768

BLAST of Csor.00g107110 vs. ExPASy TrEMBL
Match: A0A6J1I7Q4 (subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111471617 PE=3 SV=1)

HSP 1 Score: 1306 bits (3379), Expect = 0.0
Identity = 657/766 (85.77%), Postives = 685/766 (89.43%), Query Frame = 0

Query: 2   LFGFFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDA 61
           LF FF LISPVIAVKKSYIVLLGSHSHGLEVS +DLQR VDSHHKLLGSFLGSSEKAKDA
Sbjct: 10  LFWFFRLISPVIAVKKSYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGSSEKAKDA 69

Query: 62  IFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPS 121
           IFYSYKNHINGFAAIL+EEEATK AKHPEVAAVLPNKAKELHTTHSWEFMHLEKN  I  
Sbjct: 70  IFYSYKNHINGFAAILDEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFILP 129

Query: 122 SSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLKL 181
            SPWRKARFG+DVIIANLD+GVWPESKSFGEQGIVGGVPSRWKGGC DKTPD VPCN KL
Sbjct: 130 FSPWRKARFGKDVIIANLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPDAVPCNRKL 189

Query: 182 IGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIG 241
           IGAKYFNQG +AYLKSQNL +ELPLIVNSTRDY GHGSHTLSTAGG+YVS VSVFGSGIG
Sbjct: 190 IGAKYFNQGVIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSVFGSGIG 249

Query: 242 TAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDD 301
           TAKGGSPKARVAAYKVCWP  N GGC+DAD+ +  D AI+DGVDVLSLSIGSPPAEYYDD
Sbjct: 250 TAKGGSPKARVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSPPAEYYDD 309

Query: 302 IIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQR 361
            IAI SFHALKKGIPVVCSAGNSGPSMATA+NIAPWILTVGASTLDREFQAPIELGNGQR
Sbjct: 310 TIAIASFHALKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQR 369

Query: 362 FTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSS 421
           F GSSLS PLAGRKLYPLITGAQAKATTA A D++LCKP+TLD SKV GKILVCL GGSS
Sbjct: 370 FWGSSLSRPLAGRKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKILVCLTGGSS 429

Query: 422 RIDKGMQALLADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMG 481
           RIDKGMQA+LA AVGMILCNDR SGF+I+ADLHVLP SHI+YNDGQAV SYI S KNPMG
Sbjct: 430 RIDKGMQAVLAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMG 489

Query: 482 YLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFD 541
           YLIPPSSKVNTKPSP MAAFSSRGPN++SPEIIKPD+TAPGVNIIAAFSGAVSPTGEPFD
Sbjct: 490 YLIPPSSKVNTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFD 549

Query: 542 NRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGG 601
           NR VPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSA +SDNTMNLMLDGG
Sbjct: 550 NRTVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLMLDGG 609

Query: 602 SPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFKC 661
           SPIFAPATPF+YGSGHI PTGAIDPGL                                C
Sbjct: 610 SPIFAPATPFIYGSGHIHPTGAIDPGL--------------------------------C 669

Query: 662 PVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKFE 721
           PVSGSILNFNYPSIGVQNLTGSVTV+RRLKNVG PGVYRVRV++PEGVKVSVKP VLKF 
Sbjct: 670 PVSGSILNFNYPSIGVQNLTGSVTVTRRLKNVGRPGVYRVRVRRPEGVKVSVKPRVLKFW 729

Query: 722 KIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 767
           KIGEEK FELTMTGAVAEGQIGYGTLIWTD KHFV+SPIVVSS FF
Sbjct: 730 KIGEEKRFELTMTGAVAEGQIGYGTLIWTDGKHFVKSPIVVSSGFF 743

BLAST of Csor.00g107110 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 875.2 bits (2260), Expect = 3.9e-254
Identity = 442/760 (58.16%), Postives = 542/760 (71.32%), Query Frame = 0

Query: 10  SPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDAIFYSYKNH 69
           SP  A+KKSYIV LGSH+H  ++S+  L     SH   L SF+GS E AK+AIFYSYK H
Sbjct: 33  SPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRH 92

Query: 70  INGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPSSSPWRKAR 129
           INGFAAIL+E EA ++AKHP+V +V PNK ++LHTTHSW FM L KN V+  SS W KA 
Sbjct: 93  INGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAG 152

Query: 130 FGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLKLIGAKYFNQ 189
           +GED IIANLD+GVWPESKSF ++G  G VP+RWKG C       VPCN KLIGA+YFN+
Sbjct: 153 YGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRC----HKDVPCNRKLIGARYFNK 212

Query: 190 GALAY--LKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIGTAKGGS 249
           G LAY  L S    E       + RD+ GHGSHTLSTA G++V   +VFG G GTA GGS
Sbjct: 213 GYLAYTGLPSNASYE-------TCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGS 272

Query: 250 PKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDDIIAIGS 309
           PKARVAAYKVCWP  +   CFDAD+  A + AI DGVDVLS S+G    +Y  D IAIGS
Sbjct: 273 PKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGS 332

Query: 310 FHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSL 369
           FHA+K G+ VVCSAGNSGP   T SN+APW++TVGAS++DREFQA +EL NGQ F G+SL
Sbjct: 333 FHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSL 392

Query: 370 SNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSSRIDKGM 429
           S PL   K+Y LI+ A A        D++LCK  +LD  KVKGKILVCLRG ++R+DKGM
Sbjct: 393 SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGM 452

Query: 430 QALLADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMGYLIPPS 489
           QA  A A GM+LCND+ SG EI++D HVLP S I+Y DG+ +FSY+ STK+P GY+  P+
Sbjct: 453 QAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPT 512

Query: 490 SKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFDNRAVPY 549
           + +NTKP+P MA+FSSRGPN ++P I+KPD+TAPGVNIIAAF+ A  PT    DNR  P+
Sbjct: 513 ATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPF 572

Query: 550 ITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPIFAP 609
            T SGTSMSCPH+SG+VGLLK LHP WSPAAI+SAIMT++R  +N    M+D     F  
Sbjct: 573 NTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDES---FKK 632

Query: 610 ATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFT-DANFKCPVSGS 669
           A PF YGSGH++P  A  PGLVYDL+  DYL+FLCA GY    +++F  D  + C    +
Sbjct: 633 ANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGAN 692

Query: 670 ILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKFEKIGEE 729
           +L+FNYPSI V NLTGS+TV+R+LKNVG P  Y  R ++P GV+VSV+P  L F K GE 
Sbjct: 693 LLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEV 752

Query: 730 KGFELTMTG-AVAEGQIGYGTLIWTDDKHFVRSPIVVSSS 766
           K F++T+    V      +G L WTD  H+VRSPIVV  S
Sbjct: 753 KIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQLS 777

BLAST of Csor.00g107110 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 801.2 bits (2068), Expect = 7.2e-232
Identity = 407/751 (54.19%), Postives = 529/751 (70.44%), Query Frame = 0

Query: 18  SYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 77
           SY+V  G+HSH  E++   + R  ++H+  LGSF GS E+A DAIFYSY  HINGFAA L
Sbjct: 31  SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90

Query: 78  EEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPSSSPWRKARFGEDVIIA 137
           + + A +++KHPEV +V PNKA +LHTT SW+F+ LE N  +PSSS WRKARFGED IIA
Sbjct: 91  DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150

Query: 138 NLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNLKLIGAKYFNQGALAYLKS 197
           NLD+GVWPESKSF ++G+ G +PSRWKG C ++      CN KLIGA+YFN+G  A +  
Sbjct: 151 NLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGH 210

Query: 198 QNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIGTAKGGSPKARVAAYKV 257
            N   + P      RD  GHGSHTLSTA G +V  VS+FG G GTAKGGSP+ARVAAYKV
Sbjct: 211 LNSSFDSP------RDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKV 270

Query: 258 CWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDDIIAIGSFHALKKGIPV 317
           CWP      C+DADV  AFD AIHDG DV+S+S+G  P  +++D +AIGSFHA KK I V
Sbjct: 271 CWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVV 330

Query: 318 VCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSLSN-PLAGRKL 377
           VCSAGNSGP+ +T SN+APW +TVGAST+DREF + + LGNG+ + G SLS+  L   K 
Sbjct: 331 VCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKF 390

Query: 378 YPLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSSRIDKGMQALLADAVG 437
           YP++    AKA  A A D+ LCK  +LD  K KGKILVCLRG + R++KG    L   +G
Sbjct: 391 YPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIG 450

Query: 438 MILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMGYLIPPSSKVNTKPSP 497
           M+L N  ++G +++AD HVLP + +   D  AV  YI  TK P+ ++ P  + +  KP+P
Sbjct: 451 MVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAP 510

Query: 498 TMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFDNRAVPYITMSGTSMS 557
            MA+FSS+GP+IV+P+I+KPD+TAPGV++IAA++GAVSPT E FD R + +  +SGTSMS
Sbjct: 511 VMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMS 570

Query: 558 CPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPIFAPATPFVYGSG 617
           CPH+SGI GLLK  +P WSPAAI+SAIMT+A + D+    + +  +     ATPF +G+G
Sbjct: 571 CPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAG 630

Query: 618 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVFTDANFKCPVSG-SILNFNYPSI 677
           H++P  A++PGLVYDL   DYL FLC+ GY    + VF+  NF C     S++N NYPSI
Sbjct: 631 HVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSI 690

Query: 678 GVQNLTGS-VTVSRRLKNVGMPGVYRVRVQQPEGVKVSVKPNVLKFEKIGEEKGFELTMT 737
            V NLT S VTVSR +KNVG P +Y V+V  P+GV V+VKP  L F K+GE+K F++ + 
Sbjct: 691 TVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILV 750

Query: 738 ---GAVAEGQIGYGTLIWTDDKHFVRSPIVV 763
              G VA+G + +G L+W+D KH VRSPIVV
Sbjct: 751 KSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770

BLAST of Csor.00g107110 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 572.8 bits (1475), Expect = 4.2e-163
Identity = 325/732 (44.40%), Postives = 449/732 (61.34%), Query Frame = 0

Query: 42  DSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKE 101
           D H     S L S   + + + Y+Y+N I+GF+  L +EEA  L   P V +VLP    E
Sbjct: 46  DLHSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYE 105

Query: 102 LHTTHSWEFMHLEKN--DVIPSSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGV 161
           LHTT +  F+ L+++  D+ P +  +       DV++  LD+GVWPESKS+ ++G  G +
Sbjct: 106 LHTTRTPLFLGLDEHTADLFPEAGSY------SDVVVGVLDTGVWPESKSYSDEGF-GPI 165

Query: 162 PSRWKGGCMDKTP-DGVPCNLKLIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHG 221
           PS WKGGC   T      CN KLIGA++F +G   Y  +   ++E      S RD  GHG
Sbjct: 166 PSSWKGGCEAGTNFTASLCNRKLIGARFFARG---YESTMGPIDE-SKESRSPRDDDGHG 225

Query: 222 SHTLSTAGGSYVSNVSVFGSGIGTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDH 281
           +HT STA GS V   S+ G   GTA+G +P+ARVA YKVCW    +GGCF +D+  A D 
Sbjct: 226 THTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW----LGGCFSSDILAAIDK 285

Query: 282 AIHDGVDVLSLSIGSPPAEYYDDIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWI 341
           AI D V+VLS+S+G   ++YY D +AIG+F A+++GI V CSAGN+GPS ++ SN+APWI
Sbjct: 286 AIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWI 345

Query: 342 LTVGASTLDREFQAPIELGNGQRFTGSSL-SNPLAGRKLYPLITGAQAKATTAFAQDSIL 401
            TVGA TLDR+F A   LGNG+ FTG SL        KL P I    A   T    +  L
Sbjct: 346 TTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNAT----NGNL 405

Query: 402 CKPETLDHSKVKGKILVCLRGGSSRIDKGMQALLADAVGMILCNDRLSGFEIMADLHVLP 461
           C   TL   KVKGKI++C RG ++R+ KG     A  VGMIL N   +G E++AD H+LP
Sbjct: 406 CMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLP 465

Query: 462 TSHINYNDGQAVFSYIYSTKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPD 521
            + +    G  +  Y+ +  NP   +    + V  KPSP +AAFSSRGPN ++P I+KPD
Sbjct: 466 ATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPD 525

Query: 522 MTAPGVNIIAAFSGAVSPTGEPFDNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPA 581
           + APGVNI+AA++GA  PTG   D+R V +  +SGTSMSCPHVSG+  LLK++HPEWSPA
Sbjct: 526 LIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPA 585

Query: 582 AIKSAIMTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDY 641
           AI+SA+MT+A  +      +LD  +    P+TPF +G+GH+ PT A +PGL+YDL+  DY
Sbjct: 586 AIRSALMTTAYKTYKDGKPLLDIATG--KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDY 645

Query: 642 LEFLCASGYTEKNLRVFTDANFKCPVSG--SILNFNYPSIGVQ-NLTGSVTVSRRLKNVG 701
           L FLCA  YT   +R  +  N+ C  S   S+ + NYPS  V  +  G+   +R + +VG
Sbjct: 646 LGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVG 705

Query: 702 MPGVYRVRV-QQPEGVKVSVKPNVLKFEKIGEEKGFELTMT--GAVAEGQIGYGTLIWTD 761
             G Y V+V  +  GVK+SV+P VL F++  E+K + +T T   +   G   +G++ W+D
Sbjct: 706 GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSD 755

Query: 762 DKHFVRSPIVVS 764
            KH V SP+ +S
Sbjct: 766 GKHVVGSPVAIS 755

BLAST of Csor.00g107110 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 545.4 bits (1404), Expect = 7.2e-155
Identity = 335/803 (41.72%), Postives = 446/803 (55.54%), Query Frame = 0

Query: 2   LFGFFWLISPVIA----VKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSEK 61
           LF   +LI P++A     K+ YIV  G H        ++     + HH  L S   S E 
Sbjct: 7   LFPLLFLI-PLLASCAEEKQVYIVYFGEHKGDKAFHEIE-----EHHHSYLQSVKESEED 66

Query: 62  AKDAIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAK--ELHTTHSWEFMHLEK 121
           A+ ++ YSYK+ INGFAA L  ++A+KL K  EV +V  +  +  E HTT SWEF+ LE+
Sbjct: 67  ARASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEE 126

Query: 122 NDVIPSSSPWR----------------KARFGEDVIIANLDSGVWPESKSFGEQGIVGGV 181
            +   S  P R                KA+ G+ +I+  LDSGVWPESKSF ++G +G V
Sbjct: 127 EET-DSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKG-MGPV 186

Query: 182 PSRWKGGCMDKTP-DGVPCNLKLIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHG 241
           P  WKG C      +   CN K+IGA+Y+ +G   Y  + N       +  S RD  GHG
Sbjct: 187 PKSWKGICQTGVAFNSSHCNRKIIGARYYVKGYERYYGAFNATANKDFL--SPRDPDGHG 246

Query: 242 SHTLSTAGGSYVSNVSVFGS-GIGTAKGGSPKARVAAYKVCWPLPN---IGG--CFDADV 301
           SHT STA G  V   S  G    G+A GG+P AR+A YK CW  PN   + G  C + D+
Sbjct: 247 SHTASTAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDM 306

Query: 302 AEAFDHAIHDGVDVLSLSIG-SPPAEYYDDIIAIGSFHALKKGIPVVCSAGNSGPSMATA 361
             A D AI DGV V+S+SIG + P  +  D IA+G+ HA+K+ I V  SAGNSGP   T 
Sbjct: 307 LAAIDDAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTL 366

Query: 362 SNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSLSNPLAGRKLYPLITGAQAKATTAF 421
           SN+APWI+TVGASTLDR F   + LGNG      S++      K  PL+  +        
Sbjct: 367 SNLAPWIITVGASTLDRAFVGGLVLGNGYTIKTDSIT-AFKMDKFAPLVYASNVVVPGIA 426

Query: 422 AQDSILCKPETLDHSKVKGKILVCLRGGSSRIDKGMQALLADAVGMILCNDRLSGFEIMA 481
             ++  C P +L    V GK+++CLRG  SRI KGM+   A   GMIL N   +G E+ +
Sbjct: 427 LNETSQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPS 486

Query: 482 DLHVLPTSHINYNDGQAVFSYIYSTKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSP 541
           D H +PT+ +       +  YI + KNP  ++ P  +    + +P+M  FSSRGPN+V P
Sbjct: 487 DSHFVPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDP 546

Query: 542 EIIKPDMTAPGVNIIAAFSGAVSPTGEPFDNRAVPYITMSGTSMSCPHVSGIVGLLKALH 601
            I+KPD+TAPG+ I+AA+SGA SP+    D R   Y   SGTSMSCPHV+G + LLKA+H
Sbjct: 547 NILKPDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIH 606

Query: 602 PEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYD 661
           P+WS AAI+SA+MT+A ++++    + D       PA PF  GSGH RPT A DPGLVYD
Sbjct: 607 PKWSSAAIRSALMTTAWMTNDKKKPIQDTTG---LPANPFALGSGHFRPTKAADPGLVYD 666

Query: 662 LSPNDYLEFLCASGYTEKNLRVFTDANFKCPVS-GSILNFNYPSIGVQNLTGSVTVSRRL 721
            S   YL + C+   T        D  FKCP       N NYPSI V NL  +VTV R +
Sbjct: 667 ASYRAYLLYGCSVNITN------IDPTFKCPSKIPPGYNHNYPSIAVPNLKKTVTVKRTV 726

Query: 722 KNVG---MPGVYRVRVQQPEGVKVSVKPNVLKFEKIGEEKGFELT-------MTGAVAEG 764
            NVG       Y   V+ P G+ V   PN+L F +IG+++ F++        +  A  +G
Sbjct: 727 TNVGTGNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKG 786

BLAST of Csor.00g107110 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 542.0 bits (1395), Expect = 7.9e-154
Identity = 334/778 (42.93%), Postives = 458/778 (58.87%), Query Frame = 0

Query: 25  SHSHGLEVSAVDLQRA-----VDSHHKLLGSFLGS--SEKAKDAIFYSYKNHINGFAAIL 84
           S S GLE   V +QR+       SH+    S L S  S      + YSY   ++GF+A L
Sbjct: 25  SSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYSRAVHGFSARL 84

Query: 85  EEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNDVIPSSSPWRKARFGEDVIIA 144
              +   L +HP V +V+P++A+E+HTTH+  F+   +N     S  W  + +GEDVI+ 
Sbjct: 85  SPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQN-----SGLWSNSNYGEDVIVG 144

Query: 145 NLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPD--GVPCNLKLIGAKYFNQGALAYL 204
            LD+G+WPE  SF + G+ G +PS WKG C +  PD     CN KLIGA+ F +G   YL
Sbjct: 145 VLDTGIWPEHPSFSDSGL-GPIPSTWKGEC-EIGPDFPASSCNRKLIGARAFYRG---YL 204

Query: 205 KSQN-LMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIGTAKGGSPKARVAA 264
             +N   +       S RD +GHG+HT STA GS V+N S++    GTA G + KAR+AA
Sbjct: 205 TQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAA 264

Query: 265 YKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPA--EYYDDIIAIGSFHALK 324
           YK+CW     GGC+D+D+  A D A+ DGV V+SLS+G+  +  EY+ D IAIG+F A +
Sbjct: 265 YKICW----TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATR 324

Query: 325 KGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSLSNPLA 384
            GI V CSAGNSGP+  TA+NIAPWILTVGAST+DREF A    G+G+ FTG+SL    A
Sbjct: 325 HGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSL---YA 384

Query: 385 GRKLYPLITGAQAKATTAFAQD--SILCKPETLDHSKVKGKILVCLRGGSSRIDKGMQAL 444
           G  L        ++ +  ++ D  S LC P  L+ S V+GKI++C RGG++R++KG    
Sbjct: 385 GESL------PDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVK 444

Query: 445 LADAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNP------MGYLI 504
           LA   GMIL N   SG E+ AD H++P + +    G  +  YI ++ +P      +G LI
Sbjct: 445 LAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLI 504

Query: 505 PPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDMTAPGVNIIAAFSGAVSPTGEPFDNRA 564
            PS      PSP +AAFSSRGPN ++P I+KPD+ APGVNI+A ++G V PT    D R 
Sbjct: 505 GPS-----PPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRR 564

Query: 565 VPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPI 624
           V +  +SGTSMSCPHVSG+  LL+  HP+WSPAAIKSA++T+A   +N+       G PI
Sbjct: 565 VQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENS-------GEPI 624

Query: 625 FAPAT-----PFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKNLRVF-TDAN 684
              AT      F++G+GH+ P  A++PGLVYD+   +Y+ FLCA GY    + VF  D  
Sbjct: 625 EDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPT 684

Query: 685 FKCPVSGSIL----NFNYPSIGVQ-NLTGSVTVSRR-LKNVG--MPGVYRVRVQQPEGVK 744
                  S L    + NYPS  V    TG V   +R +KNVG  +  VY V V+ P  V+
Sbjct: 685 LYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVE 744

Query: 745 VSVKPNVLKFEKIGEEKGFELTMTGAVAEGQIG------YGTLIWTDDKHFVRSPIVV 763
           + V P+ L F K      +E+T    V  G +G      +G++ WTD +H V+SP+ V
Sbjct: 745 IDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAV 767