Csor.00g233590 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g233590
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionThreonyl-tRNA synthetase
LocationCsor_Chr11: 13702264 .. 13706679 (+)
RNA-Seq ExpressionCsor.00g233590
SyntenyCsor.00g233590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACTACCACTGGCTTACTCCTCCACCCTCCTCTCGTGCGACCCATTTCACCCAACGTCTCTACATCTCGTTTCTTCCTTGCAGTTTCGACCCAATTTGCAGGAAATAGGAACCAGTCTCTTTTCCCTCGCAAGAAGGCCAGTGGATTTTCGACTTCCATTGCAGTGGCTACTGATTCATCAGCGCCATCAATTTCGACCCAGAACGAAAATTTGGTGGATACCCAGAAGGAGGAAAGTGAGAAGGTTGTTCTTCCGACTAATGAATCCTCTGAGAAGCTTCTCAGAGTTCGTCACACGGTAATTCTGTTTTTTCCTTGAACTGCCCTTTAGGTGTTCGACGAAATGTCCTTTGAATATGGATATAGATTATTATCTGGGAATTTGAGAGTTTTGGATGCTTAATCAAGATTCTTAATGGGGTTTACTAGTTTGAAATTTAGGACAGAACTAGGCAGGAGTTTCATAATGGCCATGGCAATAATTGTTCAATTGACTAAATTATTGACTAAATTATGTTTGTTCAATTGCAGTGTGCCCATGTAATGGCCATGGCTGTACAGAAGCTCTTTCCTGATGCTAAAGTGACTATTGGACCATGGATAGAAAATGGGTTTTACTATGATTTTGATATGGAGCCATTGGGAGATAAGGATTTGAAGAAAATTAAGAAGGAGATGGTTGGTATACCAAATCTATGAGCACTAAATTCACTGGGACTTTTCTTCATAATTTTAGATCACAAGGTTTGGAACCTATAAACTAGTTTATGCTTTCAGGTTATGGTATTTATTGTTCAAATCATCTGTAGGAACGCATTATTAGCAGAAACTTACCACTTGTAAGAGAAGAAGTTTCAAGGGATGAAGCTCAGAGGAGAATTGAGGCAATCAACGAACCATACAAGATGGAGATTTTGGAAAGCATAAAAGAGGACCCCATAACCATCTATCATATTGGTAGGAATTCCAACCATCTTTGTACTTCTCTATTGCAACTTTTTGAGACTCCTTTGTATTTAGTGCTTGATTACTTTTGATATTTTTGTTGATTGAAGGCTTTGACCATTTTATCTTCTTTGAAATTTGGCATATGAGTTTTGAGATCCCACATCAAAGAGGAGAACGAAACATTCTTTGTAAGGGTGTGGAAACCTCTTCCCAGTAGACGCGTTTTAAAACCTTGAAGGGAAGTCCGGAAGCAAAAGCTCAAAGAGGACAATATCTGCTAGCGGTGGGCTCGAGCTGTTATAAATGGTATCAAAGCTAGACACCGGGCGATGTGCCAACGAGGAGGCTGAGCTCCGAAGGGGGGTGGATTAGGGGGTCCCACATCAATTGGAGAGGGAATGAATGCTAGCGAGGACGTTGGGCCCCGGAGGGAGAGGACGTTGAGCCTCTCCCTAGCAGACACGTTTTAAAACCTTGAGGGGAAGCCTGGAAGAGAGCACAAAGAGGACAATATTTGCTAGCGGTGGGCTTGGGCTATTACCAATGGTATCAGAGCCAGACACCGGGCGATGTGCCAGCAAGGAGACTGAGCTTCGAAGGGGGGTTGACACGAGGCGGTGTGCCAGCAAGGATGCTAGGCCTAGAAGGGGGTGGATGGGGAGGTCGTGAAATCCCACATCAACTGGGAGAATGAAACATTTTTTTATAATAGCGTGGAAACCTCTCCCTAACATACACGCGTTTTAAAACCTTGAGGGGAAGCGTATAACTAAAAGTCTGGAAGCGAAAGCCTATATGCTAGTGGTGGGCTTTAGCTGTTACAAGTTTTGAGTTTCTTTTTTCCTTTTTTCCTTTCCTGGATGCTATATTTAAATCTCTTTATTATAGGGATTTCTATTGATCTAATCCAAGTTTTTTAATCAGTAGAAAAAAGTTCTCTCCGACTTTTTTTCTCTGATATTTTTCTTTTTGGTATATTTCCTCCGTTATTTTCTCATATTTGTTAAATTATTAACAGCTTGCTATTGTTTCTGGTTCTGCAACGCTCATTCTTTTTAATCCCCTTTTCCCTCTGGAATAATGTTCGAACTTCATGTATTTGGTTGAAACTTAACATTGGAGCAACTTTGGGAATTTGCTTGCTAAATAACGAAAAGAGTGTACTCCAACTACTCCGAAAACTTTCTGTCTTTGGTATTCATCACCACATTTCATTCGTTTCGACACTAAAAGAAGGAAGGCCAAGATTGTGTTGGACATTTAGTAGCTGCAAAAGGTAGCAAATGGTGGATCAATTGTCTAGTAAATTAAGAACATCCTGTGGTGAGCTTGGTCTATTTTGATCAGCTGCTACTCCCAGGTCGAAGCTGGAAATTTTATTAGCATCTTCACTCATTTGTAATGTCCTGTATTCTTCACAGGGAATGAATGGTGGGACCTTTGTGCTGGCCCCCATGTTGAATCTACTGGGAAAATCAACAAAAGAGCTGTTGAACTTGAAACAGTTGCAGGCGCATACTGGAGAGGAGATGAAAAGAATCCGATGTTACAGAGGATATATGGCACTGCATGGGAGACAGAAGAGCAATTAAAAGCATACCTTCACTTCAAAGAGGAGGCCAGACGACGTGATCACAGACGCCTCGGAAAAGATCTTGATCTATTTTCCATTCAGGTTTAGATTTCCTCCAAAACTATTATGAAGTTCTTGCTGATAGTTAGAGTTATGCTCTTTAGATTTATTTGGGCTGAACACAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCATCCGAAGGGCGCCGTTGTGAGGAGCATAATAGAAGATTTATGGAAAAAATTACACAGGGAACGTGGTTATGATCTGCTCTATACCCCACATGTAGCAAAGGCAGAGCTTTGGAAAACCAGTGGTCATTCAGACTTCTACGCCGAAAATATGTATAATCAGATGGATGTCGAGGACGAGCTTTATCAACTTCGGCCTATGAATTGTCCTTACCATATTCTATTTTACAAAAGGAGGCTTACCTCCTATCAAGATTTTCCTATTAGGGTTGCCGAATTAGGGACGGTATATAGATATGAGCTGTCTGGTAGCTTGCACGGTCTTTTCCGTGTCAGAGGTTTCACCCAGGTGCATATATCATGGATGCTTTCATTATTCGTTTGCTTGTCCAAACTTTACAAGTTGTGATCTGCTCTACGTGGAAAAACGAAACTGATTTGGCTGCTTTACTCGTCTTTTTTGCGGATTTTGCAGGATGACGCTCACATATTTTGTTTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATCTAACAGAAGAGCTGCTACTTCAATTTGGTTTTAACAAATTTGAAGTCAATCTCTCTACTAGACCACCAAAATCAGTGGGGAATGATGATATCTGGGACAAAGCAACATCTGCTCTTAGAGATGCTCTAAGTGATAAGGGATGGGGCTATGATATTGATGAAGGTGGTGGAGCCTTTTATGGACCGAAAATCGATATTAAAATCGAGGATGCGTTGGGAAGGAAGTGGCAATGCTCAACCATACAGGCAATTTCTCAACTTTATATGGCTTAATCCAAGCAGAATTTCTTTCTCACTGTCCTTATGGGTCTGACTTTGAGTATGATCGTGGAAAACTGTTCCTTTTGTAATGTTTAAGTTCTTATTTCAGGTTGATTTTAATTTACCTCAGCGTTTCGACATCACATATGTTAATTCGAACTCCGAAAAGAAGAGACCTATCATGATTCACCGTGCGGTGCTGGGATCTCTGGAGCGATTCTTCGGTATTCTAATAGAAAATTATGCCGGAGACTTTCCGTTATGGCTTTCTCCAATTCAAGTTCGAGTTTTGCCCGTGACAGATGCCCAGGTGATTACTATATATAGGCAAAAAACCACCTGCAGTTCATGATTGTGATAGTAATCTTGTTGGACTTGTTTGACTGTCATGAAAACTTGCATGATCTTGTCTTGATAATGTGTAAAAGCTCATTGCCAACAGTCCCCATGAAGGAATTCCCAAGGGTTTCTTGGTAGAATCATACCCATGATGTCGTCATTCGAGGGGACGGGATCTCGTAAATTTGAACTGCTAACTGAATCATGTCTATGAGCTCATATTAGGTATTGAAGTTGTAATCTTCCATTGAAATCTTCATTAACATGTTTATCACAGCTCCCATACTGCAAAGAGGTGACGAAAAAACTCAAGGCTGTTGGCGTTCGAGCAGAAGTCTGTCATGGCGAGAGGTTGCCGAAGCTTATTAGAAATGCAGAGACCCAAAAAATACCGTTAATGGCGGTTGTTGGCGCCAAGGAAGTCGAGACGCAAACTGTGACGGTGAGATCAAGGTTTGGTGGCGAGATAGGGACCATAACAGTTGATGATTTTGTTGGAAGAACCAAGTTTGCCTCAGAGAATAGAACAACGTTTTGA

mRNA sequence

ATGGCGACTACCACTGGCTTACTCCTCCACCCTCCTCTCGTGCGACCCATTTCACCCAACGTCTCTACATCTCGTTTCTTCCTTGCAGTTTCGACCCAATTTGCAGGAAATAGGAACCAGTCTCTTTTCCCTCGCAAGAAGGCCAGTGGATTTTCGACTTCCATTGCAGTGGCTACTGATTCATCAGCGCCATCAATTTCGACCCAGAACGAAAATTTGGTGGATACCCAGAAGGAGGAAAGTGAGAAGGTTGTTCTTCCGACTAATGAATCCTCTGAGAAGCTTCTCAGAGTTCGTCACACGTGTGCCCATGTAATGGCCATGGCTGTACAGAAGCTCTTTCCTGATGCTAAAGTGACTATTGGACCATGGATAGAAAATGGGTTTTACTATGATTTTGATATGGAGCCATTGGGAGATAAGGATTTGAAGAAAATTAAGAAGGAGATGGAACGCATTATTAGCAGAAACTTACCACTTGTAAGAGAAGAAGTTTCAAGGGATGAAGCTCAGAGGAGAATTGAGGCAATCAACGAACCATACAAGATGGAGATTTTGGAAAGCATAAAAGAGGACCCCATAACCATCTATCATATTGGGAATGAATGGTGGGACCTTTGTGCTGGCCCCCATGTTGAATCTACTGGGAAAATCAACAAAAGAGCTGTTGAACTTGAAACAGTTGCAGGCGCATACTGGAGAGGAGATGAAAAGAATCCGATGTTACAGAGGATATATGGCACTGCATGGGAGACAGAAGAGCAATTAAAAGCATACCTTCACTTCAAAGAGGAGGCCAGACGACGTGATCACAGACGCCTCGGAAAAGATCTTGATCTATTTTCCATTCAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCATCCGAAGGGCGCCGTTGTGAGGAGCATAATAGAAGATTTATGGAAAAAATTACACAGGGAACGTGGTTATGATCTGCTCTATACCCCACATGTAGCAAAGGCAGAGCTTTGGAAAACCAGTGGTCATTCAGACTTCTACGCCGAAAATATGTATAATCAGATGGATGTCGAGGACGAGCTTTATCAACTTCGGCCTATGAATTGTCCTTACCATATTCTATTTTACAAAAGGAGGCTTACCTCCTATCAAGATTTTCCTATTAGGGTTGCCGAATTAGGGACGGTATATAGATATGAGCTGTCTGGTAGCTTGCACGGTCTTTTCCGTGTCAGAGGTTTCACCCAGGATGACGCTCACATATTTTGTTTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATCTAACAGAAGAGCTGCTACTTCAATTTGGTTTTAACAAATTTGAAGTCAATCTCTCTACTAGACCACCAAAATCAGTGGGGAATGATGATATCTGGGACAAAGCAACATCTGCTCTTAGAGATGCTCTAAGTGATAAGGGATGGGGCTATGATATTGATGAAGGTGGTGGAGCCTTTTATGGACCGAAAATCGATATTAAAATCGAGGATGCGTTGGGAAGGAAGTGGCAATGCTCAACCATACAGGTTGATTTTAATTTACCTCAGCGTTTCGACATCACATATGTTAATTCGAACTCCGAAAAGAAGAGACCTATCATGATTCACCGTGCGGTGCTGGGATCTCTGGAGCGATTCTTCGGTATTCTAATAGAAAATTATGCCGGAGACTTTCCGTTATGGCTTTCTCCAATTCAAGTTCGAGTTTTGCCCGTGACAGATGCCCAGCTCCCATACTGCAAAGAGGTGACGAAAAAACTCAAGGCTGTTGGCGTTCGAGCAGAAGTCTGTCATGGCGAGAGGTTGCCGAAGCTTATTAGAAATGCAGAGACCCAAAAAATACCGTTAATGGCGGTTGTTGGCGCCAAGGAAGTCGAGACGCAAACTGTGACGGTGAGATCAAGGTTTGGTGGCGAGATAGGGACCATAACAGTTGATGATTTTGTTGGAAGAACCAAGTTTGCCTCAGAGAATAGAACAACGTTTTGA

Coding sequence (CDS)

ATGGCGACTACCACTGGCTTACTCCTCCACCCTCCTCTCGTGCGACCCATTTCACCCAACGTCTCTACATCTCGTTTCTTCCTTGCAGTTTCGACCCAATTTGCAGGAAATAGGAACCAGTCTCTTTTCCCTCGCAAGAAGGCCAGTGGATTTTCGACTTCCATTGCAGTGGCTACTGATTCATCAGCGCCATCAATTTCGACCCAGAACGAAAATTTGGTGGATACCCAGAAGGAGGAAAGTGAGAAGGTTGTTCTTCCGACTAATGAATCCTCTGAGAAGCTTCTCAGAGTTCGTCACACGTGTGCCCATGTAATGGCCATGGCTGTACAGAAGCTCTTTCCTGATGCTAAAGTGACTATTGGACCATGGATAGAAAATGGGTTTTACTATGATTTTGATATGGAGCCATTGGGAGATAAGGATTTGAAGAAAATTAAGAAGGAGATGGAACGCATTATTAGCAGAAACTTACCACTTGTAAGAGAAGAAGTTTCAAGGGATGAAGCTCAGAGGAGAATTGAGGCAATCAACGAACCATACAAGATGGAGATTTTGGAAAGCATAAAAGAGGACCCCATAACCATCTATCATATTGGGAATGAATGGTGGGACCTTTGTGCTGGCCCCCATGTTGAATCTACTGGGAAAATCAACAAAAGAGCTGTTGAACTTGAAACAGTTGCAGGCGCATACTGGAGAGGAGATGAAAAGAATCCGATGTTACAGAGGATATATGGCACTGCATGGGAGACAGAAGAGCAATTAAAAGCATACCTTCACTTCAAAGAGGAGGCCAGACGACGTGATCACAGACGCCTCGGAAAAGATCTTGATCTATTTTCCATTCAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCATCCGAAGGGCGCCGTTGTGAGGAGCATAATAGAAGATTTATGGAAAAAATTACACAGGGAACGTGGTTATGATCTGCTCTATACCCCACATGTAGCAAAGGCAGAGCTTTGGAAAACCAGTGGTCATTCAGACTTCTACGCCGAAAATATGTATAATCAGATGGATGTCGAGGACGAGCTTTATCAACTTCGGCCTATGAATTGTCCTTACCATATTCTATTTTACAAAAGGAGGCTTACCTCCTATCAAGATTTTCCTATTAGGGTTGCCGAATTAGGGACGGTATATAGATATGAGCTGTCTGGTAGCTTGCACGGTCTTTTCCGTGTCAGAGGTTTCACCCAGGATGACGCTCACATATTTTGTTTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATCTAACAGAAGAGCTGCTACTTCAATTTGGTTTTAACAAATTTGAAGTCAATCTCTCTACTAGACCACCAAAATCAGTGGGGAATGATGATATCTGGGACAAAGCAACATCTGCTCTTAGAGATGCTCTAAGTGATAAGGGATGGGGCTATGATATTGATGAAGGTGGTGGAGCCTTTTATGGACCGAAAATCGATATTAAAATCGAGGATGCGTTGGGAAGGAAGTGGCAATGCTCAACCATACAGGTTGATTTTAATTTACCTCAGCGTTTCGACATCACATATGTTAATTCGAACTCCGAAAAGAAGAGACCTATCATGATTCACCGTGCGGTGCTGGGATCTCTGGAGCGATTCTTCGGTATTCTAATAGAAAATTATGCCGGAGACTTTCCGTTATGGCTTTCTCCAATTCAAGTTCGAGTTTTGCCCGTGACAGATGCCCAGCTCCCATACTGCAAAGAGGTGACGAAAAAACTCAAGGCTGTTGGCGTTCGAGCAGAAGTCTGTCATGGCGAGAGGTTGCCGAAGCTTATTAGAAATGCAGAGACCCAAAAAATACCGTTAATGGCGGTTGTTGGCGCCAAGGAAGTCGAGACGCAAACTGTGACGGTGAGATCAAGGTTTGGTGGCGAGATAGGGACCATAACAGTTGATGATTTTGTTGGAAGAACCAAGTTTGCCTCAGAGAATAGAACAACGTTTTGA

Protein sequence

MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATDSSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWGYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFASENRTTF
Homology
BLAST of Csor.00g233590 vs. ExPASy Swiss-Prot
Match: F4IFC5 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX=3702 GN=EMB2761 PE=2 SV=1)

HSP 1 Score: 1065.8 bits (2755), Expect = 1.9e-310
Identity = 519/665 (78.05%), Postives = 585/665 (87.97%), Query Frame = 0

Query: 1   MATTTGLLLHPP-LVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVAT 60
           MA++  LL     L +P S   S  RF    + QF        +PR++  GFST  AVAT
Sbjct: 1   MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPTRQF--------WPRQR-HGFSTVFAVAT 60

Query: 61  DSSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKV 120
           +   P+IS+        +K E   VVLP+NESS+KLL++RHTCAHVMAMAVQKLFPDAKV
Sbjct: 61  E---PAISSSG-----PKKAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKV 120

Query: 121 TIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINE 180
           TIGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPL+REEVSR+EA++RI AINE
Sbjct: 121 TIGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINE 180

Query: 181 PYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKN 240
           PYKMEIL+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK 
Sbjct: 181 PYKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKR 240

Query: 241 PMLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGA 300
            MLQRIYGTAWE+EEQLKAYLHFKEEA+RRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGA
Sbjct: 241 QMLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGA 300

Query: 301 VVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLR 360
           +VR+IIE+ WKK+H E GYDL+YTPHVAKA+LWK SGH DFY ENMY+QM++EDELYQLR
Sbjct: 301 IVRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLR 360

Query: 361 PMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLD 420
           PMNCPYHIL Y+R+  SY+D PIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCL+
Sbjct: 361 PMNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLE 420

Query: 421 DQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGW 480
           DQIKDEIRGVLDLTEE+L +FGFNK+EVNLSTRP KSVG DDIW+KAT ALRDAL DKGW
Sbjct: 421 DQIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGW 480

Query: 481 GYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMI 540
            Y++DEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITYV++NS+KKRPIMI
Sbjct: 481 SYEVDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYVDTNSDKKRPIMI 540

Query: 541 HRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAE 600
           HRAVLGSLERFFG+LIE+YAGDFPLWLSP+QVRVLPVTD QL +CKEV+KKL+A GVRAE
Sbjct: 541 HRAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKKLRACGVRAE 600

Query: 601 VCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFA 660
           +CHGERLPKLIRNAETQKIPLMAVVG KEVET TVTVRSRFGGE+GTI VDD + +   A
Sbjct: 601 LCHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVDDLINKINIA 648

Query: 661 SENRT 665
            E RT
Sbjct: 661 VETRT 648

BLAST of Csor.00g233590 vs. ExPASy Swiss-Prot
Match: A5GJT7 (Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) OX=32051 GN=thrS PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 4.3e-225
Identity = 374/614 (60.91%), Postives = 467/614 (76.06%), Query Frame = 0

Query: 52  STSIAVATDSSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQ 111
           + S A AT S++PS                  VVLP    +E+LLR+RH+ +HVMAMAVQ
Sbjct: 8   TVSSAAATTSASPS-----------------PVVLPKTSENEQLLRIRHSMSHVMAMAVQ 67

Query: 112 KLFPDAKVTIGPWIENGFYYDFD-MEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEA 171
           +LFP+A+VTIGPW E+GFYYDFD  EP  + DLK IKK M +II++ LPL R EVSR EA
Sbjct: 68  QLFPNARVTIGPWTESGFYYDFDNPEPFTEADLKAIKKGMIKIINKKLPLERVEVSRSEA 127

Query: 172 QRRIEAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAG 231
           + +I+A NEPYK+EILE ++E PIT+Y +G +WWDLCAGPHVE+TG++N +A ELE+VAG
Sbjct: 128 EEKIKAQNEPYKLEILEGLQE-PITLYTLGEDWWDLCAGPHVENTGQLNAKAFELESVAG 187

Query: 232 AYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGG 291
           AYWRGDE    LQRIYGTAWET EQL  +   KEEA RRDHRR+GKDLDLFSI+D+AG G
Sbjct: 188 AYWRGDETKAQLQRIYGTAWETAEQLAEHKRRKEEALRRDHRRIGKDLDLFSIEDEAGAG 247

Query: 292 LVFWHPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMD 351
           LVFWHP+GA +R +IE+ W++ H E GY+LLYTPHVA   LWKTSGH DFYAE+M+  M+
Sbjct: 248 LVFWHPRGARMRLLIEEFWRQAHFEGGYELLYTPHVADISLWKTSGHLDFYAESMFGPME 307

Query: 352 VEDELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQ 411
           V++  YQL+PMNCP+H+L Y  +L SY++ PIR AELGTVYRYE  G +HGL RVRGFTQ
Sbjct: 308 VDEREYQLKPMNCPFHVLTYASKLRSYRELPIRWAELGTVYRYERPGVMHGLMRVRGFTQ 367

Query: 412 DDAHIFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSAL 471
           DDAH+FCL +QI DEI  +LDLTE +L  F FN +E+NLSTRP KS+G+D +WD AT  L
Sbjct: 368 DDAHVFCLPEQISDEILKILDLTERILSTFDFNTYEINLSTRPEKSIGDDAVWDLATKGL 427

Query: 472 RDALSDKGWGYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSN 531
            +AL  KGW Y IDEGGGAFYGPKID+KIEDA+GR WQCSTIQ+DFNLP+RF + Y+ ++
Sbjct: 428 IEALERKGWAYKIDEGGGAFYGPKIDLKIEDAIGRMWQCSTIQLDFNLPERFKLDYIAAD 487

Query: 532 SEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKK 591
             K+RPIMIHRA+ GSLERFFGI+ ENYAGD+PLWL+P QVR+LPVTD   PY + +  +
Sbjct: 488 GSKQRPIMIHRAIFGSLERFFGIMTENYAGDYPLWLAPEQVRLLPVTDEVQPYAESLLDQ 547

Query: 592 LKAVGVRAEVCH-GERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITV 651
           L   GVRA +   G+RL KLIR  E  KIP++AV+GAKE E   V++RSR  G++G + V
Sbjct: 548 LTQAGVRATIDRSGDRLGKLIRTGEQMKIPVLAVIGAKEAEQNAVSLRSRRDGDLGVVAV 603

Query: 652 DDFVGRTKFASENR 664
            D +   + A+  R
Sbjct: 608 ADLLRAAQNANSQR 603

BLAST of Csor.00g233590 vs. ExPASy Swiss-Prot
Match: Q0I902 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) OX=64471 GN=thrS PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 9.5e-225
Identity = 366/582 (62.89%), Postives = 456/582 (78.35%), Query Frame = 0

Query: 84  VVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD-MEPLGDKD 143
           VVLP    SE LL++RH+ +HVMAMAVQKLFP A+VTIGPW E GFYYDFD  EP  + D
Sbjct: 23  VVLPKTSESENLLKIRHSMSHVMAMAVQKLFPKAQVTIGPWTEAGFYYDFDNPEPFTEAD 82

Query: 144 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMEILESIKEDPITIYHIGNE 203
           LK IKKEM +II R LPL R EVSR+EA+RRI+A NEPYK+EILE + E PIT+Y +G +
Sbjct: 83  LKAIKKEMGKIIGRKLPLERIEVSREEAERRIKAQNEPYKLEILERLVE-PITLYTLGEQ 142

Query: 204 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 263
           WWDLCAGPHV +T ++N +A ELE+VAGAYWRGDE    LQRIYGTAWET +QL  +   
Sbjct: 143 WWDLCAGPHVANTSELNPKAFELESVAGAYWRGDETKAQLQRIYGTAWETADQLSEHKRR 202

Query: 264 KEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDLLY 323
           KEEA RRDHRRLGKDLDLFSI+D+AG GLVFWHP+GA +R +IED W++ H E GY+LLY
Sbjct: 203 KEEALRRDHRRLGKDLDLFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262

Query: 324 TPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRPMNCPYHILFYKRRLTSYQDFPI 383
           TPHVA   LWKTSGH DFYAE+M+  M+V++  YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDERQYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 384 RVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGF 443
           R AELGTVYRYE  G +HGL RVRGFTQDDAH+FCL +QI DEI  +L+LTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILRILNLTERILSTFDF 382

Query: 444 NKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWGYDIDEGGGAFYGPKIDIKIEDA 503
           + +E+NLST+P K++G+D +W+ AT  L +AL  KGW Y IDEGGGAFYGPKID+KIEDA
Sbjct: 383 SNYEINLSTKPDKAIGDDAVWELATKGLIEALKRKGWAYKIDEGGGAFYGPKIDLKIEDA 442

Query: 504 LGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 563
           +GR WQCSTIQ+DFNLP+RF++ Y+ ++  K+RPIMIHRA+ GSLERFFGI+ ENYAGDF
Sbjct: 443 IGRMWQCSTIQLDFNLPERFELDYIAADGSKQRPIMIHRAIFGSLERFFGIMTENYAGDF 502

Query: 564 PLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEVCH-GERLPKLIRNAETQKIPLM 623
           P WL+P Q+R+LPVTD  L Y +E   +LKA G+RA +   G+RL KLIR  E  KIP++
Sbjct: 503 PFWLAPEQIRLLPVTDEVLGYAEEFQNQLKAAGIRASIDRSGDRLGKLIRIGEKMKIPVL 562

Query: 624 AVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFASENR 664
           AV+GAKE E    ++RSR  G++G IT +  +   + A+++R
Sbjct: 563 AVIGAKEAEQGAASLRSRRDGDLGVITKERLIATAQSANQDR 603

BLAST of Csor.00g233590 vs. ExPASy Swiss-Prot
Match: Q3AYC6 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) OX=316279 GN=thrS PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 1.6e-224
Identity = 366/582 (62.89%), Postives = 456/582 (78.35%), Query Frame = 0

Query: 84  VVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD-MEPLGDKD 143
           VVLP    SE+LL++RH+ +HVMAMAVQKLFP+A+VTIGPW E GFYYDFD  EP  + D
Sbjct: 23  VVLPKTSESEQLLKIRHSMSHVMAMAVQKLFPNAQVTIGPWTETGFYYDFDNPEPFTEDD 82

Query: 144 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMEILESIKEDPITIYHIGNE 203
           LK IKKEM +II R LPL R EVSRDEA+ RI+A NEPYK+EILE ++E PIT+Y +G++
Sbjct: 83  LKAIKKEMGKIIGRKLPLERIEVSRDEAETRIKAQNEPYKLEILERLQE-PITLYTLGDQ 142

Query: 204 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 263
           WWDLCAGPHVE+T +++ +A ELE+VAGAYWRGDE    LQRIYGTAWET EQL  +   
Sbjct: 143 WWDLCAGPHVENTKELHPKAFELESVAGAYWRGDETKAQLQRIYGTAWETPEQLAEHKRR 202

Query: 264 KEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDLLY 323
           K EA RRDHRRLGKDL+LFSI+D+AG GLVFWHP+GA +R +IED W++ H E GY+LLY
Sbjct: 203 KVEALRRDHRRLGKDLELFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262

Query: 324 TPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRPMNCPYHILFYKRRLTSYQDFPI 383
           TPHVA   LWKTSGH DFYAE+M+  M V++  YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMQVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 384 RVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGF 443
           R AELGTVYRYE  G +HGL RVRGFTQDDAH+FCL DQI DEI  +LDLTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPDQISDEILRILDLTERILSTFDF 382

Query: 444 NKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWGYDIDEGGGAFYGPKIDIKIEDA 503
           N +E+NLSTRP KS+G++ +W+ AT  L +AL  KGW + IDEGGGAFYGPKID+KIEDA
Sbjct: 383 NHYEINLSTRPEKSIGSEAVWELATKGLTEALERKGWNFKIDEGGGAFYGPKIDLKIEDA 442

Query: 504 LGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 563
           +GR WQCSTIQ+DFNLP+RF + YV ++  K++PIMIHRA+ GSLERFFGI+ ENYAGDF
Sbjct: 443 IGRMWQCSTIQLDFNLPERFGLDYVAADGSKQQPIMIHRAIFGSLERFFGIMTENYAGDF 502

Query: 564 PLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEVCH-GERLPKLIRNAETQKIPLM 623
           P WL+P Q+R+LPVTD   PY ++V  +LK  G+RA +   G+RL K+IR  E  KIP++
Sbjct: 503 PFWLAPEQIRLLPVTDDVQPYAEQVLNQLKTAGIRATIDQSGDRLGKIIRTGEQMKIPVL 562

Query: 624 AVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFASENR 664
           AV+GAKE E   +++RSR  G++G  +VD  +   + A+  R
Sbjct: 563 AVIGAKEAEQNAISLRSRRDGDLGVTSVDALLKAAQRANAER 603

BLAST of Csor.00g233590 vs. ExPASy Swiss-Prot
Match: Q3AKU0 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) OX=110662 GN=thrS PE=3 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 1.5e-222
Identity = 362/590 (61.36%), Postives = 455/590 (77.12%), Query Frame = 0

Query: 76  TQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFD- 135
           T    S  VVLP    S++LL++RH+ +HVMAMAVQ+LFP A+VTIGPW E GFYYDFD 
Sbjct: 15  TTPAPSAPVVLPKTSESDQLLKIRHSMSHVMAMAVQQLFPKARVTIGPWTETGFYYDFDN 74

Query: 136 MEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMEILESIKEDPI 195
            +P  + DLK IKK M +II++ LPL R EVSR+EA+ +I+A NEPYK+EIL+ + E PI
Sbjct: 75  PDPFTEADLKAIKKGMIKIINKKLPLQRVEVSRNEAEEKIKAQNEPYKLEILQGLHE-PI 134

Query: 196 TIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEE 255
           T+Y +G +WWDLCAGPHV+ TG++N +A ELE++AGAYWRGDE    LQRIYGTAWE+ E
Sbjct: 135 TLYTLGEDWWDLCAGPHVDHTGQLNAKAFELESLAGAYWRGDETKAQLQRIYGTAWESPE 194

Query: 256 QLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHR 315
           QL  +   KEEA RRDHRR+GKDLDLFSI+D+AG GLVFWHP+GA +R +IE+ W++ H 
Sbjct: 195 QLAEHKRRKEEALRRDHRRIGKDLDLFSIEDEAGAGLVFWHPRGARIRLLIEEFWRQAHF 254

Query: 316 ERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRPMNCPYHILFYKRRL 375
           E GY+LLYTPHVA   LWKTSGH DFYAE+M+  M+V++  YQL+PMNCP+H+L Y  +L
Sbjct: 255 EGGYELLYTPHVADISLWKTSGHLDFYAESMFGPMEVDEREYQLKPMNCPFHVLTYASKL 314

Query: 376 TSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTE 435
            SY++ PIR AELGTVYRYE  G +HGL RVRGFTQDDAH+FCL +QI DEI  +LDLTE
Sbjct: 315 RSYRELPIRWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILKILDLTE 374

Query: 436 ELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWGYDIDEGGGAFYGPK 495
            +L  F F+ +E+NLSTRP KS+G D +WD AT  L +AL  KGW Y IDEGGGAFYGPK
Sbjct: 375 RILSAFDFSNYEINLSTRPEKSIGEDAVWDLATKGLIEALERKGWAYKIDEGGGAFYGPK 434

Query: 496 IDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERFFGIL 555
           ID+KIEDA+GR WQCSTIQ+DFNLP+RF++ Y+ ++  K+RPIMIHRA+ GSLERFFGI+
Sbjct: 435 IDLKIEDAIGRMWQCSTIQLDFNLPERFELDYIAADGSKQRPIMIHRAIFGSLERFFGIM 494

Query: 556 IENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEVCH-GERLPKLIRNA 615
            ENYAGD+P WL+P QVR+LPVTD   PY +++  +L   GVRA V   G+RL KLIR  
Sbjct: 495 TENYAGDYPFWLAPEQVRLLPVTDEVQPYAEQLLDQLTKAGVRATVDRSGDRLGKLIRTG 554

Query: 616 ETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFASENR 664
           E  KIP++AV+GAKE E   V++RSR  G++G   V D +   + A+  R
Sbjct: 555 EQMKIPVLAVIGAKEAEQNAVSLRSRRDGDLGVTAVSDLLSAAQMANSER 603

BLAST of Csor.00g233590 vs. NCBI nr
Match: KAG6589116.1 (Threonine--tRNA ligase, chloroplastic/mitochondrial 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022820.1 Threonine--tRNA ligase, chloroplastic/mitochondrial 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1342 bits (3473), Expect = 0.0
Identity = 666/666 (100.00%), Postives = 666/666 (100.00%), Query Frame = 0

Query: 1   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD 60
           MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD
Sbjct: 1   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD 60

Query: 61  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 120
           SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 61  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 120

Query: 121 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 180
           IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP
Sbjct: 121 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 180

Query: 181 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 240
           YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP
Sbjct: 181 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 240

Query: 241 MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 300
           MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV
Sbjct: 241 MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 300

Query: 301 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 360
           VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP
Sbjct: 301 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 360

Query: 361 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 420
           MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD
Sbjct: 361 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 420

Query: 421 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 480
           QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG
Sbjct: 421 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 480

Query: 481 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 540
           YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH
Sbjct: 481 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 540

Query: 541 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 600
           RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV
Sbjct: 541 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 600

Query: 601 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS 660
           CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS
Sbjct: 601 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS 660

Query: 661 ENRTTF 666
           ENRTTF
Sbjct: 661 ENRTTF 666

BLAST of Csor.00g233590 vs. NCBI nr
Match: XP_022928511.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita moschata])

HSP 1 Score: 1335 bits (3454), Expect = 0.0
Identity = 661/666 (99.25%), Postives = 665/666 (99.85%), Query Frame = 0

Query: 1   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD 60
           MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRN+SL+PRKKASGFSTSIAVATD
Sbjct: 7   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNKSLYPRKKASGFSTSIAVATD 66

Query: 61  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 120
           SSAPSISTQN+NLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 67  SSAPSISTQNDNLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 126

Query: 121 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 180
           IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP
Sbjct: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186

Query: 181 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 240
           YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP
Sbjct: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246

Query: 241 MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 300
           MLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV
Sbjct: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306

Query: 301 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 360
           VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP
Sbjct: 307 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 366

Query: 361 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 420
           MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD
Sbjct: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426

Query: 421 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 480
           QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG
Sbjct: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 486

Query: 481 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 540
           YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH
Sbjct: 487 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 546

Query: 541 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 600
           RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV
Sbjct: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 606

Query: 601 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS 660
           CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGR KFAS
Sbjct: 607 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRIKFAS 666

Query: 661 ENRTTF 666
           ENRTTF
Sbjct: 667 ENRTTF 672

BLAST of Csor.00g233590 vs. NCBI nr
Match: XP_023529641.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1328 bits (3436), Expect = 0.0
Identity = 658/666 (98.80%), Postives = 663/666 (99.55%), Query Frame = 0

Query: 1   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD 60
           MATTTGLLLHPPLVRPIS NVSTSRFFLAVSTQFAGNRN+SLF RKKASGFSTSIAVATD
Sbjct: 7   MATTTGLLLHPPLVRPISSNVSTSRFFLAVSTQFAGNRNKSLFTRKKASGFSTSIAVATD 66

Query: 61  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 120
           SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLR+RHTCAHVMAMAVQKLFPDAKVT
Sbjct: 67  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126

Query: 121 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 180
           IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQ+RIEAINEP
Sbjct: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQKRIEAINEP 186

Query: 181 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 240
           YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP
Sbjct: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246

Query: 241 MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 300
           MLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV
Sbjct: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306

Query: 301 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 360
           VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP
Sbjct: 307 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 366

Query: 361 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 420
           MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD
Sbjct: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426

Query: 421 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 480
           QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG
Sbjct: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 486

Query: 481 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 540
           YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH
Sbjct: 487 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 546

Query: 541 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 600
           RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV
Sbjct: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 606

Query: 601 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS 660
           CHGERLPKLIRNAET+KIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGR KFAS
Sbjct: 607 CHGERLPKLIRNAETKKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRIKFAS 666

Query: 661 ENRTTF 666
           ENRTTF
Sbjct: 667 ENRTTF 672

BLAST of Csor.00g233590 vs. NCBI nr
Match: XP_022989354.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita maxima] >XP_022989355.1 threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita maxima])

HSP 1 Score: 1322 bits (3422), Expect = 0.0
Identity = 657/666 (98.65%), Postives = 661/666 (99.25%), Query Frame = 0

Query: 1   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD 60
           MATTTGLLLH PLVRPISPNVSTSRFFLAVSTQFAG RN+SLFPRKKASG STSIAVATD
Sbjct: 7   MATTTGLLLHLPLVRPISPNVSTSRFFLAVSTQFAGIRNKSLFPRKKASGISTSIAVATD 66

Query: 61  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 120
           SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLR+RHTCAHVMAMAVQKLFPDAKVT
Sbjct: 67  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126

Query: 121 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 180
           IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP
Sbjct: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186

Query: 181 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 240
           YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP
Sbjct: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246

Query: 241 MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 300
           MLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV
Sbjct: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306

Query: 301 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 360
           VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP
Sbjct: 307 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 366

Query: 361 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 420
           MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD
Sbjct: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426

Query: 421 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 480
           QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG
Sbjct: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 486

Query: 481 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 540
           YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH
Sbjct: 487 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 546

Query: 541 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 600
           RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPY KEVTKKLKAVG+RAEV
Sbjct: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYWKEVTKKLKAVGIRAEV 606

Query: 601 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS 660
           CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGR KFAS
Sbjct: 607 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRIKFAS 666

Query: 661 ENRTTF 666
           ENRTTF
Sbjct: 667 ENRTTF 672

BLAST of Csor.00g233590 vs. NCBI nr
Match: XP_022952560.1 (threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita moschata])

HSP 1 Score: 1293 bits (3345), Expect = 0.0
Identity = 636/666 (95.50%), Postives = 655/666 (98.35%), Query Frame = 0

Query: 1   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD 60
           MA+TTGLLLHPPLVRPISPNVST RFFLAV+TQFAGNR++SLF RKKA+GFSTSIAVATD
Sbjct: 7   MASTTGLLLHPPLVRPISPNVSTPRFFLAVATQFAGNRDKSLFHRKKANGFSTSIAVATD 66

Query: 61  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 120
           SSAPSI TQNE+LVDT KEESEK+VLPTNESSEKLLR+RH+CAHVMAMAVQKLFPDAKVT
Sbjct: 67  SSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLFPDAKVT 126

Query: 121 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 180
           IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP
Sbjct: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186

Query: 181 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 240
           YKMEILESIKEDPITI+HIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP
Sbjct: 187 YKMEILESIKEDPITIFHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246

Query: 241 MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 300
           MLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV
Sbjct: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306

Query: 301 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 360
           VRSIIEDLWKKLHRERGYDLLYTPHVAKA LWKTSGHSDFY+ENMYNQMDVEDELYQLRP
Sbjct: 307 VRSIIEDLWKKLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDELYQLRP 366

Query: 361 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 420
           MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD
Sbjct: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426

Query: 421 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 480
           QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KAT AL+DALSDKGWG
Sbjct: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDALSDKGWG 486

Query: 481 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 540
           Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH
Sbjct: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 546

Query: 541 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 600
           RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVT+KL+A+GVRAEV
Sbjct: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTEKLRAIGVRAEV 606

Query: 601 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS 660
           C GERLPKLIRNAETQKIPLMAVVGAKEVET+TVTVRSRFGGEIGTITVDDF GR KFAS
Sbjct: 607 CQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEIGTITVDDFAGRIKFAS 666

Query: 661 ENRTTF 666
           ENRT F
Sbjct: 667 ENRTPF 672

BLAST of Csor.00g233590 vs. ExPASy TrEMBL
Match: A0A6J1EKH5 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435298 PE=3 SV=1)

HSP 1 Score: 1335 bits (3454), Expect = 0.0
Identity = 661/666 (99.25%), Postives = 665/666 (99.85%), Query Frame = 0

Query: 1   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD 60
           MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRN+SL+PRKKASGFSTSIAVATD
Sbjct: 7   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNKSLYPRKKASGFSTSIAVATD 66

Query: 61  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 120
           SSAPSISTQN+NLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 67  SSAPSISTQNDNLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 126

Query: 121 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 180
           IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP
Sbjct: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186

Query: 181 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 240
           YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP
Sbjct: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246

Query: 241 MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 300
           MLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV
Sbjct: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306

Query: 301 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 360
           VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP
Sbjct: 307 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 366

Query: 361 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 420
           MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD
Sbjct: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426

Query: 421 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 480
           QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG
Sbjct: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 486

Query: 481 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 540
           YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH
Sbjct: 487 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 546

Query: 541 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 600
           RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV
Sbjct: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 606

Query: 601 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS 660
           CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGR KFAS
Sbjct: 607 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRIKFAS 666

Query: 661 ENRTTF 666
           ENRTTF
Sbjct: 667 ENRTTF 672

BLAST of Csor.00g233590 vs. ExPASy TrEMBL
Match: A0A6J1JP39 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111486433 PE=3 SV=1)

HSP 1 Score: 1322 bits (3422), Expect = 0.0
Identity = 657/666 (98.65%), Postives = 661/666 (99.25%), Query Frame = 0

Query: 1   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD 60
           MATTTGLLLH PLVRPISPNVSTSRFFLAVSTQFAG RN+SLFPRKKASG STSIAVATD
Sbjct: 7   MATTTGLLLHLPLVRPISPNVSTSRFFLAVSTQFAGIRNKSLFPRKKASGISTSIAVATD 66

Query: 61  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 120
           SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLR+RHTCAHVMAMAVQKLFPDAKVT
Sbjct: 67  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126

Query: 121 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 180
           IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP
Sbjct: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186

Query: 181 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 240
           YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP
Sbjct: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246

Query: 241 MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 300
           MLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV
Sbjct: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306

Query: 301 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 360
           VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP
Sbjct: 307 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 366

Query: 361 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 420
           MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD
Sbjct: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426

Query: 421 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 480
           QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG
Sbjct: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 486

Query: 481 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 540
           YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH
Sbjct: 487 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 546

Query: 541 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 600
           RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPY KEVTKKLKAVG+RAEV
Sbjct: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYWKEVTKKLKAVGIRAEV 606

Query: 601 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS 660
           CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGR KFAS
Sbjct: 607 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRIKFAS 666

Query: 661 ENRTTF 666
           ENRTTF
Sbjct: 667 ENRTTF 672

BLAST of Csor.00g233590 vs. ExPASy TrEMBL
Match: A0A6J1GM45 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1)

HSP 1 Score: 1293 bits (3345), Expect = 0.0
Identity = 636/666 (95.50%), Postives = 655/666 (98.35%), Query Frame = 0

Query: 1   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD 60
           MA+TTGLLLHPPLVRPISPNVST RFFLAV+TQFAGNR++SLF RKKA+GFSTSIAVATD
Sbjct: 7   MASTTGLLLHPPLVRPISPNVSTPRFFLAVATQFAGNRDKSLFHRKKANGFSTSIAVATD 66

Query: 61  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 120
           SSAPSI TQNE+LVDT KEESEK+VLPTNESSEKLLR+RH+CAHVMAMAVQKLFPDAKVT
Sbjct: 67  SSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLFPDAKVT 126

Query: 121 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 180
           IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP
Sbjct: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186

Query: 181 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 240
           YKMEILESIKEDPITI+HIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP
Sbjct: 187 YKMEILESIKEDPITIFHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246

Query: 241 MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 300
           MLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV
Sbjct: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306

Query: 301 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 360
           VRSIIEDLWKKLHRERGYDLLYTPHVAKA LWKTSGHSDFY+ENMYNQMDVEDELYQLRP
Sbjct: 307 VRSIIEDLWKKLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDELYQLRP 366

Query: 361 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 420
           MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD
Sbjct: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426

Query: 421 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 480
           QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KAT AL+DALSDKGWG
Sbjct: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDALSDKGWG 486

Query: 481 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 540
           Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH
Sbjct: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 546

Query: 541 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 600
           RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVT+KL+A+GVRAEV
Sbjct: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTEKLRAIGVRAEV 606

Query: 601 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS 660
           C GERLPKLIRNAETQKIPLMAVVGAKEVET+TVTVRSRFGGEIGTITVDDF GR KFAS
Sbjct: 607 CQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEIGTITVDDFAGRIKFAS 666

Query: 661 ENRTTF 666
           ENRT F
Sbjct: 667 ENRTPF 672

BLAST of Csor.00g233590 vs. ExPASy TrEMBL
Match: A0A6J1HZS8 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468143 PE=3 SV=1)

HSP 1 Score: 1284 bits (3323), Expect = 0.0
Identity = 633/666 (95.05%), Postives = 651/666 (97.75%), Query Frame = 0

Query: 1   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD 60
           MA+TTGLLLHPPLVRPISPNVS  RFFLAV+TQFAGNR++SLF RKKA+GFSTSIAVATD
Sbjct: 7   MASTTGLLLHPPLVRPISPNVSAPRFFLAVATQFAGNRDKSLFHRKKANGFSTSIAVATD 66

Query: 61  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 120
           SSAPSI TQNE+LVDT KEESEK+VLPTNESSEKLLR+RH+CAHVMAMAVQKLFPDAKVT
Sbjct: 67  SSAPSIPTQNEDLVDTHKEESEKIVLPTNESSEKLLRIRHSCAHVMAMAVQKLFPDAKVT 126

Query: 121 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 180
           IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSR EAQRRIEAINEP
Sbjct: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRGEAQRRIEAINEP 186

Query: 181 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 240
           YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKN 
Sbjct: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNQ 246

Query: 241 MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 300
           MLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV
Sbjct: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306

Query: 301 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 360
           VRSIIEDLWK LHRERGYDLLYTPHVAKA LWKTSGHSDFY+ENMYNQMDVEDELYQLRP
Sbjct: 307 VRSIIEDLWKTLHRERGYDLLYTPHVAKAALWKTSGHSDFYSENMYNQMDVEDELYQLRP 366

Query: 361 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 420
           MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD
Sbjct: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426

Query: 421 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 480
           QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KAT AL+DALSDKGWG
Sbjct: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATCALKDALSDKGWG 486

Query: 481 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 540
           Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH
Sbjct: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 546

Query: 541 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 600
           RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVT+KL+A+GVRAEV
Sbjct: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTEKLRAIGVRAEV 606

Query: 601 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS 660
           C GERLPKLIRNAETQKIPLMAVVGAKEVET+TVTVRSRFGGEIGTITVDDF GR KFAS
Sbjct: 607 CQGERLPKLIRNAETQKIPLMAVVGAKEVETRTVTVRSRFGGEIGTITVDDFAGRIKFAS 666

Query: 661 ENRTTF 666
           ENRT F
Sbjct: 667 ENRTPF 672

BLAST of Csor.00g233590 vs. ExPASy TrEMBL
Match: A0A6J1C3B9 (Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111007557 PE=3 SV=1)

HSP 1 Score: 1281 bits (3314), Expect = 0.0
Identity = 629/666 (94.44%), Postives = 653/666 (98.05%), Query Frame = 0

Query: 1   MATTTGLLLHPPLVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVATD 60
           MA++TGLLL+PPLVRPIS NVS  RFFLAV+TQFAGNR++SLF  KKA+GFS+S+AVATD
Sbjct: 7   MASSTGLLLNPPLVRPISSNVSPHRFFLAVATQFAGNRDKSLFHSKKANGFSSSVAVATD 66

Query: 61  SSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKVT 120
           SS  S+STQNE+LV+TQKEESEKVVLPTNESSEKLLR+RHTCAHVMAMAVQKLFPDAKVT
Sbjct: 67  SSTTSVSTQNEDLVETQKEESEKVVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126

Query: 121 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 180
           IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL+REEVSRDEAQRRI AINEP
Sbjct: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLLREEVSRDEAQRRIMAINEP 186

Query: 181 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 240
           YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP
Sbjct: 187 YKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246

Query: 241 MLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 300
           MLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGA+
Sbjct: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAI 306

Query: 301 VRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLRP 360
           VRSIIEDLWKKLHR+RGYDLLYTPHVAKAELWKTSGHSDFY ENMYNQMDVEDELYQLRP
Sbjct: 307 VRSIIEDLWKKLHRQRGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVEDELYQLRP 366

Query: 361 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 420
           MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD
Sbjct: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDD 426

Query: 421 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWG 480
           QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGND+IW+KATSALRDALSDKGW 
Sbjct: 427 QIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDEIWEKATSALRDALSDKGWS 486

Query: 481 YDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 540
           Y IDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH
Sbjct: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIH 546

Query: 541 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEV 600
           RAVLGSLERFFGILIENYAGDFPLWLSP+QVRVLPVTD+QLPYCKEVT+KLKAVGVRAE 
Sbjct: 547 RAVLGSLERFFGILIENYAGDFPLWLSPVQVRVLPVTDSQLPYCKEVTEKLKAVGVRAEF 606

Query: 601 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFAS 660
           CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVG+ KFAS
Sbjct: 607 CHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGKIKFAS 666

Query: 661 ENRTTF 666
           ENRT+F
Sbjct: 667 ENRTSF 672

BLAST of Csor.00g233590 vs. TAIR 10
Match: AT2G04842.1 (threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative )

HSP 1 Score: 1065.8 bits (2755), Expect = 1.4e-311
Identity = 519/665 (78.05%), Postives = 585/665 (87.97%), Query Frame = 0

Query: 1   MATTTGLLLHPP-LVRPISPNVSTSRFFLAVSTQFAGNRNQSLFPRKKASGFSTSIAVAT 60
           MA++  LL     L +P S   S  RF    + QF        +PR++  GFST  AVAT
Sbjct: 1   MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPTRQF--------WPRQR-HGFSTVFAVAT 60

Query: 61  DSSAPSISTQNENLVDTQKEESEKVVLPTNESSEKLLRVRHTCAHVMAMAVQKLFPDAKV 120
           +   P+IS+        +K E   VVLP+NESS+KLL++RHTCAHVMAMAVQKLFPDAKV
Sbjct: 61  E---PAISSSG-----PKKAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKV 120

Query: 121 TIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINE 180
           TIGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPL+REEVSR+EA++RI AINE
Sbjct: 121 TIGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINE 180

Query: 181 PYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKN 240
           PYKMEIL+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK 
Sbjct: 181 PYKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKR 240

Query: 241 PMLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGA 300
            MLQRIYGTAWE+EEQLKAYLHFKEEA+RRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGA
Sbjct: 241 QMLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGA 300

Query: 301 VVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYAENMYNQMDVEDELYQLR 360
           +VR+IIE+ WKK+H E GYDL+YTPHVAKA+LWK SGH DFY ENMY+QM++EDELYQLR
Sbjct: 301 IVRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLR 360

Query: 361 PMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLD 420
           PMNCPYHIL Y+R+  SY+D PIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCL+
Sbjct: 361 PMNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLE 420

Query: 421 DQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGW 480
           DQIKDEIRGVLDLTEE+L +FGFNK+EVNLSTRP KSVG DDIW+KAT ALRDAL DKGW
Sbjct: 421 DQIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGW 480

Query: 481 GYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMI 540
            Y++DEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITYV++NS+KKRPIMI
Sbjct: 481 SYEVDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYVDTNSDKKRPIMI 540

Query: 541 HRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAE 600
           HRAVLGSLERFFG+LIE+YAGDFPLWLSP+QVRVLPVTD QL +CKEV+KKL+A GVRAE
Sbjct: 541 HRAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKKLRACGVRAE 600

Query: 601 VCHGERLPKLIRNAETQKIPLMAVVGAKEVETQTVTVRSRFGGEIGTITVDDFVGRTKFA 660
           +CHGERLPKLIRNAETQKIPLMAVVG KEVET TVTVRSRFGGE+GTI VDD + +   A
Sbjct: 601 LCHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVDDLINKINIA 648

Query: 661 SENRT 665
            E RT
Sbjct: 661 VETRT 648

BLAST of Csor.00g233590 vs. TAIR 10
Match: AT5G26830.1 (Threonyl-tRNA synthetase )

HSP 1 Score: 407.1 bits (1045), Expect = 2.6e-113
Identity = 221/565 (39.12%), Postives = 337/565 (59.65%), Query Frame = 0

Query: 100 HTCAHVMAMAVQKLFPDAKVTIGPWIE--NGFYYDFDMEPLG--DKDLKKIKKEMERIIS 159
           H+ AH++  A+++ +   ++ IGP      GFYYD     LG  D     I+    +   
Sbjct: 147 HSSAHILGQALEQEY-GCQLCIGPCTTRGEGFYYDGFYGELGLSDNHFPSIEAGAAKAAK 206

Query: 160 RNLPLVREEVSRDEAQRRIEAINEPYKMEILESIKED-PITIYHIGNEWWDLCAGPHVES 219
              P  R EV++D+A       N  +K+E++  +  D  IT+Y  G    DLC GPH+ +
Sbjct: 207 EAQPFERIEVTKDQALEMFSENN--FKVELINGLPADMTITVYRCG-PLVDLCRGPHIPN 266

Query: 220 TGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRL 279
           T  +  +A +    + AYW+GD+    LQR+YG ++  ++QLK YL F EEA++ DHR L
Sbjct: 267 TSFV--KAFKCLRASSAYWKGDKDRESLQRVYGISYPDQKQLKKYLQFLEEAKKYDHRLL 326

Query: 280 GKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKT 339
           G+  +LF        G  F+ P G  V + + D  K  +  RGY  + TP++   ELW+T
Sbjct: 327 GQKQELF-FSHQLSPGSYFFLPLGTRVYNRLMDFIKNQYWHRGYTEVITPNMYNMELWQT 386

Query: 340 SGHSDFYAENMYNQMDVEDELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYE 399
           SGH+D Y +NM+   ++E + + L+PMNCP H L ++ R+ SY++ P+R+A+ G ++R E
Sbjct: 387 SGHADNYKDNMFT-FNIEKQEFGLKPMNCPGHCLIFQHRVRSYRELPMRLADFGVLHRNE 446

Query: 400 LSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQFGFNKFEVNLSTRPP 459
            SG+L GL RVR F QDDAHIFC  +Q+K E++GVL+  + +   FGF  +E+ LSTRP 
Sbjct: 447 ASGALSGLTRVRRFQQDDAHIFCTTEQVKGEVQGVLEFIDYVYKVFGFT-YELKLSTRPE 506

Query: 460 KSVGNDDIWDKATSALRDALSDKGWGYDIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQV 519
           K +G+ + WDKA + L++A+   G    ++EG GAFYGPKIDI + DA+ RK+QC+T+Q+
Sbjct: 507 KYLGDLETWDKAEADLKEAIEAFGKPLVLNEGDGAFYGPKIDITVSDAMNRKFQCATLQL 566

Query: 520 DFNLPQRFDITYVNSNSEKK-RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRV 579
           DF LP RF++ Y   +  KK RP+MIHRAVLGS+ER F IL+E+Y G +P W+SP Q  V
Sbjct: 567 DFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWPFWISPRQAIV 626

Query: 580 LPVTDAQLPYCKEVTKKLKAVGVRAEVCHGER-LPKLIRNAETQKIPLMAVVGAKEVETQ 639
            P+++    Y ++V K++K  G   +    +R + K +R A+  +   + VVG  E  T 
Sbjct: 627 CPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYILVVGETEAATG 686

Query: 640 TVTVRSRFGGEIGTITVDDFVGRTK 658
            V+VR R        +++D +   K
Sbjct: 687 QVSVRVRDNAAHSVKSIEDLLEEFK 702

BLAST of Csor.00g233590 vs. TAIR 10
Match: AT1G17960.1 (Threonyl-tRNA synthetase )

HSP 1 Score: 240.0 bits (611), Expect = 5.6e-63
Identity = 128/287 (44.60%), Postives = 180/287 (62.72%), Query Frame = 0

Query: 381 PIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEELLLQF 440
           PIR+AE G ++R E SG+L G+ RVR F QDDAHIFC  DQ+++E++GVLD  + +   F
Sbjct: 171 PIRMAEFGVLHRNEDSGALGGMTRVRRFVQDDAHIFCRVDQVEEEVKGVLDFIDYVYRIF 230

Query: 441 GFNKFEVNLSTRPPKSVGNDDIWDKATSALRDALSDKGWGYDIDEGGGAFYGPKIDIKIE 500
           GF  +E+ LSTRP   +G+ + W KA + L  AL D G  + I EG GAFYGPKIDI + 
Sbjct: 231 GFT-YELTLSTRPKDHIGDLETWAKAENDLEKALDDFGKPWVIKEGDGAFYGPKIDITVS 290

Query: 501 DALGRKWQCSTIQVDFNLPQRFDITYVNSNSEKKRPIMIHRAVLGSLERFFGILIENYAG 560
           DA  RK+QC+TIQ+DF LP  F + Y++  +E + P+MIH AVLGS+ER F IL+E+Y G
Sbjct: 291 DARNRKFQCATIQLDFQLPACFKLKYLSEKNEMEAPVMIHSAVLGSIERMFAILLEHYKG 350

Query: 561 DFPLWLSPIQVRVLPVTDAQLPYCKEVTKKLKAVGVRAEVCHGER-LPKLIRNAETQKIP 620
            +P WLSP Q  V  +++    Y K+V K++  VG   ++   +R L K + +A      
Sbjct: 351 IWPFWLSPRQAIVCSLSEDCSSYAKQVQKQINEVGYYVDIDESDRSLRKKVADARDAPYN 410

Query: 621 LMAVVGAKEVETQTVTVRSRFGGE----IGTITVDDFVGRTKFASEN 663
            + VVG KEV T  VTVR R   E    +  ++++  +   KF + N
Sbjct: 411 YILVVGQKEVATGQVTVRLREDPEGRKDLPEMSIESLLDEFKFKTVN 456

BLAST of Csor.00g233590 vs. TAIR 10
Match: AT1G18130.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 1.3e-14
Identity = 47/125 (37.60%), Postives = 67/125 (53.60%), Query Frame = 0

Query: 514 VDFNLPQRFDITYVNSNSEK-KRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVR 573
           VDF+L  RF + Y   N    +R ++IHRAVL S+ER F IL+E Y G FP WLSP Q  
Sbjct: 17  VDFHLRDRFKLEYSAENETMIERSVLIHRAVLSSVERLFAILLEQYKGKFPFWLSPRQAI 76

Query: 574 VLPVTDAQLPYCKEVTKKLKAVGVRAEVCHGER-LPKLIRNAETQKIPLMAVVGAKEVET 633
           V  V++    Y  +V  ++   G   +V   +R +   ++ A+      + VVG +E  T
Sbjct: 77  VCSVSEDYRSYADKVRDQIHEAGYYVDVDTTDRNVSDKVQEAQVAHYNYILVVGDEEART 136

Query: 634 QTVTV 637
             VTV
Sbjct: 137 GQVTV 141

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IFC51.9e-31078.05Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana OX... [more]
A5GJT74.3e-22560.91Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) OX=32051 GN=thrS PE=... [more]
Q0I9029.5e-22562.89Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) OX=64471 GN=thrS PE=... [more]
Q3AYC61.6e-22462.89Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) OX=316279 GN=thrS PE... [more]
Q3AKU01.5e-22261.36Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) OX=110662 GN=thrS PE... [more]
Match NameE-valueIdentityDescription
KAG6589116.10.0100.00Threonine--tRNA ligase, chloroplastic/mitochondrial 2, partial [Cucurbita argyro... [more]
XP_022928511.10.099.25threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita moschata][more]
XP_023529641.10.098.80threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita pepo subsp... [more]
XP_022989354.10.098.65threonine--tRNA ligase, chloroplastic/mitochondrial 2-like [Cucurbita maxima] >X... [more]
XP_022952560.10.095.50threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1EKH50.099.25Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111435298 PE=3 SV=1[more]
A0A6J1JP390.098.65Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111486433 PE=3 SV=1[more]
A0A6J1GM450.095.50Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111455212 PE=3 SV=1[more]
A0A6J1HZS80.095.05Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468143 PE=3 SV=1[more]
A0A6J1C3B90.094.44Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111007557 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT2G04842.11.4e-31178.05threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative [more]
AT5G26830.12.6e-11339.12Threonyl-tRNA synthetase [more]
AT1G17960.15.6e-6344.60Threonyl-tRNA synthetase [more]
AT1G18130.11.3e-1437.60Class II aaRS and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002320Threonine-tRNA ligase, class IIaPRINTSPR01047TRNASYNTHTHRcoord: 493..521
score: 57.02
coord: 358..386
score: 46.55
coord: 391..414
score: 67.11
coord: 536..549
score: 68.62
coord: 563..575
score: 60.71
IPR002320Threonine-tRNA ligase, class IIaTIGRFAMTIGR00418TIGR00418coord: 99..656
e-value: 1.8E-218
score: 725.0
IPR002320Threonine-tRNA ligase, class IIaPANTHERPTHR11451THREONINE-TRNA LIGASEcoord: 57..664
IPR002320Threonine-tRNA ligase, class IIaHAMAPMF_00184Thr_tRNA_synthcoord: 93..662
score: 33.18729
IPR012947Threonyl/alanyl tRNA synthetase, SADSMARTSM00863tRNA_SAD_4coord: 194..246
e-value: 2.4E-21
score: 86.9
IPR012947Threonyl/alanyl tRNA synthetase, SADPFAMPF07973tRNA_SADcoord: 194..246
e-value: 3.2E-13
score: 49.6
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 252..561
e-value: 3.6E-121
score: 406.1
NoneNo IPR availableGENE3D3.30.54.20coord: 156..212
e-value: 3.0E-60
score: 205.1
NoneNo IPR availableGENE3D3.30.980.10coord: 97..250
e-value: 3.0E-60
score: 205.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 62..81
NoneNo IPR availablePANTHERPTHR11451:SF44THREONINE--TRNA LIGASE, CHLOROPLASTIC/MITOCHONDRIAL 2coord: 57..664
NoneNo IPR availableCDDcd00860ThrRS_anticodoncoord: 568..657
e-value: 8.16771E-34
score: 122.227
NoneNo IPR availableSUPERFAMILY52954Class II aaRS ABD-relatedcoord: 558..655
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 270..555
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 570..654
e-value: 4.0E-18
score: 65.4
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 350..558
e-value: 2.1E-40
score: 138.6
IPR036621Anticodon-binding domain superfamilyGENE3D3.40.50.800coord: 562..665
e-value: 9.9E-27
score: 95.4
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 294..563
score: 25.799753
IPR033728Threonine-tRNA ligase catalytic core domainCDDcd00771ThrRS_corecoord: 270..568
e-value: 0.0
score: 521.726
IPR018163Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamilySUPERFAMILY55186ThrRS/AlaRS common domaincoord: 91..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g233590.m01Csor.00g233590.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006435 threonyl-tRNA aminoacylation
biological_process GO:0043039 tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004829 threonine-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding