Csor.00g292860 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g292860
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	amidase 1-like isoform X2
Location	Csor_Chr10: 8704041 .. 8707683 (+)
RNA-Seq Expression	Csor.00g292860
Synteny	Csor.00g292860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintroninternalterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCATCTCTTTCCTTCAACATTAACCATGTTCCTGCCATGTTAAAAAGGTACATTTCTTTGACACCATCTTCTCATTTTAGGTTGGTTTGCCATCGATTCTGCCGTATTCAAAAGAGTAGGTCGGCTGCTGCTGCAAGAGCCAGAGGTTGAACACTACAAGCCTACGCAGGTGCTGATTGCAGAAGATTGTTTCAAGCTCTCAAGCATTCCTAGTGAGCGATTGACACAAGCTTTTGTTAATTCAGTGAAGAAGCTATTTGGTGGTAAGCCTGACACCGTTATTTTGTTTTTTTGATTAGTACCATGCTTTCATTGAGGATGAATGAAAAAAATACATGGGCATACAAAAAAAAAATAGTTCAGAAAGTGAGGAGCCAAGCTAAACTAAAAAGGGACTCCAATCAAGCATAATAAGGCCTAAAGTGTAATTATGAAAGTTTTTGAACACCAAGCCCAAAGAGAGATATTAAACCTAACAAGGGGCTAATTCTCCTAGAGAACTCATTAACCCATTAGCATTCTATTGTTTCTCTCAAGCCAAAGGCTTCATAAAACTGCACAACAATGGGGCAATGAGTATCAGTTATTGCAAAGGTTATGGTCTTGAGGAATGGATATTTGATATTGGTTAGTGAATGAATCAGGACTTGATTTTCTTCTCATTCTTTCAGAGAGAACTCATTTGAGATCTGGCTTCTTATTTGAAATATCTGGACTGTAGCCTTTTTGGCACATTCAACAAAAGGTCGATTTAGAGCCAAAGGTTTGCAAGCGGCTAAGAAACACTTCTTTATTGAATTAACAAAACCCTTTGTTAGTTATTTATCTATGAGTTATCTTTGTAACCAAATATAGTAGGGTTAGATTTATTAAAAAATGACGTGTCTGTGAGTCAATAAGCTTGACTTGTACATGATTTATTCTTTGCACGGTTTATTAATAGCAGAGTATTTATAGGTGAAGTTATTAATCATGGAGATGGAGCATTTGTAGGAGAAGTTATTAGTAGTGGAACATTTCTAGAAATATTAGTCCTGGAACAATTTTAGGAGAAATCACTGGTAGTGGAGCATTTCTAAGAGAGGTTATTAGTTGTGGAGCATTTTTACGAGAAGAATTGTTAGTGCTTCTTCCCTTTAAATAGCCTACGTTCTATTCACAACAAAAATTTACTCTCCCCTCGAGTTATCGTGTTATTTTTTTTCGCTTCTTTTTGTTTCGATTATTTGTTTCAATGTGCTTCGATCGGATGTCAAAACCATCATCTAGTATCAGACCTAGGATGCCGAAACAAATCCCGTCATCGCATAAAGTCATTGGGGGAGATGTGACGACGATACAGAACAGGAAGGAGAGGAGTGTAACGCTCACATACCCGTTACTCACAAAGAGTAACTATGTAGCGTGATCGATAAACATGCGTGTTAATTTGTTAGCACATGGTGTGTGGGTCGCCATCAAACATGGTGACAATATCGAGGAGCACAAGGACAAGATGACTCTTGTTGCCATCTACCAAGCAGTCGCGGAGGATGTCCTTCTCATGTTGGCAGAGAAAGACTCGACAAAGGCAGCATGGGAAACACCGCAAACAATGCATATGGGTGTATAATGGGTCAAGGAAGCAAAAGTGCAGATTTTGATGGGTGATTTCAAGGCTATCCGCATGAAAGACGGTTAGTCGGTGAACGACTTTACCATGAAGTTAACGATGATCGTCAACGGTATCTACTTGTTAAGAGACAAGGTGGAGGAGATCTCCTTCATCAAGAAGTTCCTCCAAGCTGTCTCCGAGATCATACAAATTGTTGCCTCCATTGAGTAGTCAACAACCTCAAGAACATGTCGGTTAAGGAGGTCGACAACCTTAAGAAAAAATGTTAACTAATTTGGAGTTCATCTGTGCTTTCAGATATAAATTTTCAATTGGGATGGATGATTACTTATGTAAAACTTCAACTACAGGCCATCTTATAAAATAGGCCTTGGGAATACTTGAATTCATCTCTGCTTTCGAATATAAATTTTCAATTGTTATGTACAATTACTAACTTGAGCCTTCAACTGCAGACCATCTTATAAAACAGATTAGCATCGGGAGTTATGTTGAGGACAAAGTTCCAAGTTTGAAGCATTTCATGATTGAAGAAAACGATGGCTATAAGCATATCATTCCATCCTTAGCTGCTCTTGTGAGGTCTAAGGTATTGCTTCAGAGGTATTGATCTTTGGATGAACTGCTACTCAAATTCTGTCGAGATAATTGATTGTGATGTATAATTGTGTTGAATTAAATGATGATCTTCACCCTCTTTTTATGCTTGTAGGCATGAGTTCAAAATCAATCACGGAGAATGGGTTAGGTCCTATAGTTCTCATTTGGGCCCAGGAAAATCAGAACGAATATCAGAATACATCAGGGTGGCAACGGATGAAAATACTGATCTAAGTCGCTCCGTTCAAATTGAACTGCGTGTAGCTCTTGCTGCTCTTCTTGAAGTATTATCTCTCTCTCTCTCACACAATTTACTAAAATTTTAAACTGAAATAATTCCAACTTAAAAATATTTGCATTTTGATTTCTACTGTTAACTTCTTTTGCTTTTTGTGTGGAAAAAACTCCCAATGACTAAATATGTGTTCTACGAGACGAATTGAATTAATTTTAATCAGGAATATTAATGGTAGCTATCTGTAATTTTGTCAGACTTTTTCTTTTGTGGTCAGGAGGGAGTTAGGGTAGGAAGAATTTTAGTTTAGTTTGTTGAAGATCGTTAGGAGAGGAGTCCCAAGTCTGTTAATTCACGGGTTGATCATGGGTTTATACGAGACATTAAGGAGAAACCAAAAGCAAAATCATGAGAGCTTATGTTTAAAGTGAACAATATCATACTATTGTGAAGAGTCGTGATTTCTAACCTGGTGCCAGAGTCATGCCCTTAACTTAGCCATGTCAATAGAGTCCTTAAATGTCAAACAAAGAAGTTGAGAGTTTCGAATGTGTAGTCAAAAGTGACTCGAGTGCCGAAGTGAAGGTGTAGTCAAAAGTGACTCAAGTGTCGAACAAAGGGTTTTCTTTGGATGGGGGCTCTAGAGAAGGAGTCGAGTCTCAATTAAGGGGAGGTTGTTCGAAGGCTCCATAGATCTCAGGGGAAGCTCTATGATATACTTTGTTCGAGGGGAGGATTGTTGAGATTTGTTGGAGGAGTCCCACGTTAGCTAATTAAGGAATGATCATGAGTTTATAAGTAATGAATACATATTGTCCTACTTTCATTTCAGGATTTCGGAGTTCTTGTAATTCCTACAGTCCCAGGTCCCCCTCCGAAACTAAGAACAGACATCTCGGAGCTACACGACTTTCGTGCAAAGGCTTTCAGTTTGCTCTCCATTGCTTCGGTCTCCGGAATCTGCCAGGTTCTCTCTCACTCTTTCTCTCTCTCTCTTGAAAGTTAAAGCAACCATATTGATGTATTTCTTGCCACCAACTAATGTGCAGGCTACCATACCTCTAGGACTGTACAACGGCCTTCCTGTGTCTATATCTTTGCTAGCAAAACATGGTTCAGATGGGTTTTTGCTCAATGTTGTTGACACTCTTTACAGCACTCTAAAACAAGAAGTTGAGGCAAGCTACTAA

mRNA sequence

ATGTCATCTCTTTCCTTCAACATTAACCATGTTCCTGCCATGTTAAAAAGAGTAGGTCGGCTGCTGCTGCAAGAGCCAGAGGTTGAACACTACAAGCCTACGCAGGTGCTGATTGCAGAAGATTGTTTCAAGCTCTCAAGCATTCCTAGTGAGCGATTGACACAAGCTTTTGTTAATTCAGTGAAGAAGCTATTTGGTGACCATCTTATAAAACAGATTAGCATCGGGAGTTATGTTGAGGACAAAGTTCCAAGTTTGAAGCATTTCATGATTGAAGAAAACGATGGCTATAAGCATATCATTCCATCCTTAGCTGCTCTTGTGAGGTCTAAGGTATTGCTTCAGAGGCATGAGTTCAAAATCAATCACGGAGAATGGGTTAGGTCCTATAGTTCTCATTTGGGCCCAGGAAAATCAGAACGAATATCAGAATACATCAGGGTGGCAACGGATGAAAATACTGATCTAAGTCGCTCCGTTCAAATTGAACTGCGTGTAGCTCTTGCTGCTCTTCTTGAAGATTTCGGAGTTCTTGTAATTCCTACAGTCCCAGGTCCCCCTCCGAAACTAAGAACAGACATCTCGGAGCTACACGACTTTCGTGCAAAGGCTTTCAGTTTGCTCTCCATTGCTTCGGTCTCCGGAATCTGCCAGGCTACCATACCTCTAGGACTGTACAACGGCCTTCCTGTGTCTATATCTTTGCTAGCAAAACATGGTTCAGATGGGTTTTTGCTCAATGTTGTTGACACTCTTTACAGCACTCTAAAACAAGAAGTTGAGGCAAGCTACTAA

Coding sequence (CDS)

ATGTCATCTCTTTCCTTCAACATTAACCATGTTCCTGCCATGTTAAAAAGAGTAGGTCGGCTGCTGCTGCAAGAGCCAGAGGTTGAACACTACAAGCCTACGCAGGTGCTGATTGCAGAAGATTGTTTCAAGCTCTCAAGCATTCCTAGTGAGCGATTGACACAAGCTTTTGTTAATTCAGTGAAGAAGCTATTTGGTGACCATCTTATAAAACAGATTAGCATCGGGAGTTATGTTGAGGACAAAGTTCCAAGTTTGAAGCATTTCATGATTGAAGAAAACGATGGCTATAAGCATATCATTCCATCCTTAGCTGCTCTTGTGAGGTCTAAGGTATTGCTTCAGAGGCATGAGTTCAAAATCAATCACGGAGAATGGGTTAGGTCCTATAGTTCTCATTTGGGCCCAGGAAAATCAGAACGAATATCAGAATACATCAGGGTGGCAACGGATGAAAATACTGATCTAAGTCGCTCCGTTCAAATTGAACTGCGTGTAGCTCTTGCTGCTCTTCTTGAAGATTTCGGAGTTCTTGTAATTCCTACAGTCCCAGGTCCCCCTCCGAAACTAAGAACAGACATCTCGGAGCTACACGACTTTCGTGCAAAGGCTTTCAGTTTGCTCTCCATTGCTTCGGTCTCCGGAATCTGCCAGGCTACCATACCTCTAGGACTGTACAACGGCCTTCCTGTGTCTATATCTTTGCTAGCAAAACATGGTTCAGATGGGTTTTTGCTCAATGTTGTTGACACTCTTTACAGCACTCTAAAACAAGAAGTTGAGGCAAGCTACTAA

Protein sequence

MSSLSFNINHVPAMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTLYSTLKQEVEASY

Homology

[Close]

BLAST of Csor.00g292860 vs. ExPASy Swiss-Prot
Match: Q9FR37 (Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.2e-67
Identity = 135/243 (55.56%), Postives = 176/243 (72.43%), Query Frame = 0


Query: 13  AMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH-LIK 72
           A LKRVG +LLQ+  +   +P+Q++IA+DCFKL S+P + L Q  V SV+K FG + ++K
Sbjct: 180 ATLKRVGCVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVVK 239

Query: 73  QISIGSYVEDKVPSLKHFMIEEN--DGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRS 132
           ++++G Y+   VPSLKHFM  ++     +  IPSL AL  S  LLQRHEFKINHG W+ S
Sbjct: 240 KVNLGEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISS 299

Query: 133 YSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPK 192
                GPG SERI E IR  +DE  D  RSV+ EL  AL+ LL + GVLVIPTVPGPPP 
Sbjct: 300 VKPEFGPGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPH 359

Query: 193 LRTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVV 252
           L+ +++ L  FR++AFSLLSIA VSG CQ +IPLGL+  LPVS+SL+AK+GSDGFLL++V
Sbjct: 360 LQANVAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLV 419

[Close]

BLAST of Csor.00g292860 vs. ExPASy Swiss-Prot
Match: Q7XTK3 (Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 6.3e-55
Identity = 111/250 (44.40%), Postives = 161/250 (64.40%), Query Frame = 0


Query: 15  LKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQIS 74
           L RV ++LL  P+    +PTQV I  DCF++     +R  Q    SV K F   ++   +
Sbjct: 184 LSRVTKVLLPLPDDIVKQPTQVTIPMDCFQILGSLDDRTYQIINASVAKRFDSQILDNRN 243

Query: 75  IGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSSHL 134
           +G ++ D VPS+  F+ + ++     +P+L+ +      LQR +FK NH EWV +   +L
Sbjct: 244 LGDFISDNVPSIGKFITDFSESELPSVPALSVISHVMRGLQRSQFKANHAEWVNTVKPNL 303

Query: 135 GPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRTDI 194
           GPG  ERI E I    +E+ +  ++++ E + ALAALL+D G+L IPTVPGPPPK+  + 
Sbjct: 304 GPGLRERILEAIASGDNESLEDFQAIRAEFKSALAALLKDHGILAIPTVPGPPPKVGMEA 363

Query: 195 SELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTLYS 254
           + L +FRA+AFSLLSIA +SG CQ +IPLG+ NGLPVS+SL+A+HG+D FLLNVV+ LY 
Sbjct: 364 APLENFRARAFSLLSIAGLSGFCQVSIPLGMRNGLPVSVSLVARHGADHFLLNVVEELYQ 423

Query: 255 TLKQEVEASY 265
           TL  E   ++
Sbjct: 424 TLIDEATKTW 433

[Close]

BLAST of Csor.00g292860 vs. ExPASy Swiss-Prot
Match: Q9MUK5 (Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=TOC64 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 5.0e-52
Identity = 109/250 (43.60%), Postives = 161/250 (64.40%), Query Frame = 0


Query: 12  PAMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIK 71
           P +L+RVG +LLQ P V    P Q++IA+DCF+  ++P +R +Q  + + +KLFG  ++K
Sbjct: 212 PDVLRRVGHILLQAPFVMQRNPRQIIIADDCFQHLNVPLDRTSQVVIKATEKLFGKQVLK 271

Query: 72  QISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYS 131
            I+   Y+  KV SLK   I++++G      SL  L      LQRHEF+  H EW+    
Sbjct: 272 HINFEDYISSKVSSLKACSIQKSNGVLK-SSSLKLLANVMQSLQRHEFEHTHSEWMSIVK 331

Query: 132 SHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLR 191
             L P  S ++ E   V+  E  + S+SV+ ELRVA+ +LL+D GVLVIPTV  PPPKL 
Sbjct: 332 PDLHPAVSAQLHEKFEVSELE-IENSKSVRSELRVAVNSLLKDEGVLVIPTVADPPPKLG 391

Query: 192 TDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDT 251
                 HD++++A SLLSIAS+SG CQ T+PLG ++  PVS+SL+A+HG D FLL+ + T
Sbjct: 392 GKEFLSHDYQSRALSLLSIASISGCCQVTVPLGFFDKNPVSVSLIARHGGDRFLLDTLKT 451

Query: 252 LYSTLKQEVE 262
           +Y+ L+++ +
Sbjct: 452 MYTVLQEQAD 459

[Close]

BLAST of Csor.00g292860 vs. ExPASy Swiss-Prot
Match: Q9LVH5 (Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP64 PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 5.2e-49
Identity = 103/247 (41.70%), Postives = 152/247 (61.54%), Query Frame = 0


Query: 12  PAMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIK 71
           P  L+RVG +LLQ P      P Q+++A+DCF+L  IP +R+TQ    S +KLFG  L+K
Sbjct: 209 PNTLRRVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKLFGRQLLK 268

Query: 72  QISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYS 131
             ++ +Y E KVPSLK F   +      +  S   L     LLQRHEF  NHG+W+ +  
Sbjct: 269 HQNLETYFETKVPSLKEFARTKAIANTKVSTS-RLLANVMQLLQRHEFLQNHGDWINTVK 328

Query: 132 SHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLR 191
             + P    ++ E   + T+E T+   +++ E RVA+ +LL+D G+LVIPT+P  PPKL 
Sbjct: 329 PAIDPVILSQVCENPEL-TNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLG 388

Query: 192 TDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDT 251
           +      D++ +A SLLSIAS+SG CQ T+PLG +   P+S+S + +HG D FLL+ V T
Sbjct: 389 SKEITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQT 448

Query: 252 LYSTLKQ 259
           +Y +L++
Sbjct: 449 MYPSLQE 453

[Close]

BLAST of Csor.00g292860 vs. ExPASy Swiss-Prot
Match: F4KCL7 (Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 9.8e-40
Identity = 92/251 (36.65%), Postives = 148/251 (58.96%), Query Frame = 0


Query: 12  PAMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIK 71
           P++L +VG  LL    V H +   ++ A+D F+LS IP ++  Q    +++ L G    K
Sbjct: 222 PSVLCQVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENLSGYKTPK 281

Query: 72  QISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYS 131
            +++G YV   VPSL  F  E++   ++   +L AL    + +QRHEFK NH EW ++  
Sbjct: 282 HVNVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCK 341

Query: 132 SHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLR 191
           S LGP  S  +   ++   +    L R V+ E+R  + +LL++ G+LVIPTV  PPP+L 
Sbjct: 342 SFLGPRFSNDVVTALKSKNESIKSLYR-VKNEMRATIQSLLKEDGILVIPTVADPPPRLN 401

Query: 192 TDISE-LHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD 251
           T  ++ L++F  + ++L  IAS+SG CQ TIPLG +   P+S+SLL  +G D FLL+   
Sbjct: 402 TKRNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTL 461

Query: 252 TLYSTLKQEVE 262
            +Y++L+ + +
Sbjct: 462 DVYASLQDQAK 470

[Close]

BLAST of Csor.00g292860 vs. NCBI nr
Match: KAG6590387.1 (Amidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 517 bits (1332), Expect = 7.95e-185
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0


Query: 1   MSSLSFNINHVPAMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNS 60
           MSSLSFNINHVPAMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNS
Sbjct: 1   MSSLSFNINHVPAMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNS 60

Query: 61  VKKLFGDHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFK 120
           VKKLFGDHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFK
Sbjct: 61  VKKLFGDHLIKQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFK 120

Query: 121 INHGEWVRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVI 180
           INHGEWVRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVI
Sbjct: 121 INHGEWVRSYSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVI 180

Query: 181 PTVPGPPPKLRTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHG 240
           PTVPGPPPKLRTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHG
Sbjct: 181 PTVPGPPPKLRTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHG 240

Query: 241 SDGFLLNVVDTLYSTLKQEVEASY 264
           SDGFLLNVVDTLYSTLKQEVEASY
Sbjct: 241 SDGFLLNVVDTLYSTLKQEVEASY 264

[Close]

BLAST of Csor.00g292860 vs. NCBI nr
Match: XP_022961361.1 (amidase 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 491 bits (1263), Expect = 9.73e-172
Identity = 250/252 (99.21%), Postives = 251/252 (99.60%), Query Frame = 0


Query: 13  AMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ 72
           A+ KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ
Sbjct: 178 AVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ 237

Query: 73  ISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSS 132
           ISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSS
Sbjct: 238 ISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSS 297

Query: 133 HLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRT 192
           HLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRT
Sbjct: 298 HLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRT 357

Query: 193 DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL 252
           DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL
Sbjct: 358 DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL 417

Query: 253 YSTLKQEVEASY 264
           YSTLKQEVEASY
Sbjct: 418 YSTLKQEVEASY 429

[Close]

BLAST of Csor.00g292860 vs. NCBI nr
Match: XP_022961359.1 (amidase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 486 bits (1250), Expect = 9.89e-170
Identity = 250/254 (98.43%), Postives = 251/254 (98.82%), Query Frame = 0


Query: 13  AMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG--DHLI 72
           A+ KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG  DHLI
Sbjct: 178 AVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGAADHLI 237

Query: 73  KQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSY 132
           KQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSY
Sbjct: 238 KQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSY 297

Query: 133 SSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKL 192
           SSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKL
Sbjct: 298 SSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKL 357

Query: 193 RTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD 252
           RTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD
Sbjct: 358 RTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD 417

Query: 253 TLYSTLKQEVEASY 264
           TLYSTLKQEVEASY
Sbjct: 418 TLYSTLKQEVEASY 431

[Close]

BLAST of Csor.00g292860 vs. NCBI nr
Match: XP_022968724.1 (amidase 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 484 bits (1247), Expect = 2.64e-169
Identity = 247/252 (98.02%), Postives = 248/252 (98.41%), Query Frame = 0


Query: 13  AMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ 72
           A+ KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ
Sbjct: 178 AVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ 237

Query: 73  ISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSS 132
           ISIGSYVEDKVPSLKHFMIEENDGYKH IPSLAALVRSKVLLQRHEFKINHGEWVRSYSS
Sbjct: 238 ISIGSYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKVLLQRHEFKINHGEWVRSYSS 297

Query: 133 HLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRT 192
           HLGPGKSERISEYIRVATDENTDLS SVQIELRVALAALLEDFGVLVIPTVPGPPPKL T
Sbjct: 298 HLGPGKSERISEYIRVATDENTDLSHSVQIELRVALAALLEDFGVLVIPTVPGPPPKLNT 357

Query: 193 DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL 252
           DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL
Sbjct: 358 DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL 417

Query: 253 YSTLKQEVEASY 264
           YSTLKQEVEASY
Sbjct: 418 YSTLKQEVEASY 429

[Close]

BLAST of Csor.00g292860 vs. NCBI nr
Match: XP_022968723.1 (amidase 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 479 bits (1234), Expect = 2.68e-167
Identity = 247/254 (97.24%), Postives = 248/254 (97.64%), Query Frame = 0


Query: 13  AMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG--DHLI 72
           A+ KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG  DHLI
Sbjct: 178 AVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGAADHLI 237

Query: 73  KQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSY 132
           KQISIGSYVEDKVPSLKHFMIEENDGYKH IPSLAALVRSKVLLQRHEFKINHGEWVRSY
Sbjct: 238 KQISIGSYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKVLLQRHEFKINHGEWVRSY 297

Query: 133 SSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKL 192
           SSHLGPGKSERISEYIRVATDENTDLS SVQIELRVALAALLEDFGVLVIPTVPGPPPKL
Sbjct: 298 SSHLGPGKSERISEYIRVATDENTDLSHSVQIELRVALAALLEDFGVLVIPTVPGPPPKL 357

Query: 193 RTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD 252
            TDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD
Sbjct: 358 NTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD 417

Query: 253 TLYSTLKQEVEASY 264
           TLYSTLKQEVEASY
Sbjct: 418 TLYSTLKQEVEASY 431

[Close]

BLAST of Csor.00g292860 vs. ExPASy TrEMBL
Match: A0A6J1HA58 (amidase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461951 PE=3 SV=1)

HSP 1 Score: 491 bits (1263), Expect = 4.71e-172
Identity = 250/252 (99.21%), Postives = 251/252 (99.60%), Query Frame = 0


Query: 13  AMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ 72
           A+ KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ
Sbjct: 178 AVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ 237

Query: 73  ISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSS 132
           ISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSS
Sbjct: 238 ISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSS 297

Query: 133 HLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRT 192
           HLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRT
Sbjct: 298 HLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRT 357

Query: 193 DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL 252
           DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL
Sbjct: 358 DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL 417

Query: 253 YSTLKQEVEASY 264
           YSTLKQEVEASY
Sbjct: 418 YSTLKQEVEASY 429

[Close]

BLAST of Csor.00g292860 vs. ExPASy TrEMBL
Match: A0A6J1HDT1 (amidase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461951 PE=3 SV=1)

HSP 1 Score: 486 bits (1250), Expect = 4.79e-170
Identity = 250/254 (98.43%), Postives = 251/254 (98.82%), Query Frame = 0


Query: 13  AMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG--DHLI 72
           A+ KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG  DHLI
Sbjct: 178 AVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGAADHLI 237

Query: 73  KQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSY 132
           KQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSY
Sbjct: 238 KQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSY 297

Query: 133 SSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKL 192
           SSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKL
Sbjct: 298 SSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKL 357

Query: 193 RTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD 252
           RTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD
Sbjct: 358 RTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD 417

Query: 253 TLYSTLKQEVEASY 264
           TLYSTLKQEVEASY
Sbjct: 418 TLYSTLKQEVEASY 431

[Close]

BLAST of Csor.00g292860 vs. ExPASy TrEMBL
Match: A0A6J1I0H5 (amidase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1)

HSP 1 Score: 484 bits (1247), Expect = 1.28e-169
Identity = 247/252 (98.02%), Postives = 248/252 (98.41%), Query Frame = 0


Query: 13  AMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ 72
           A+ KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ
Sbjct: 178 AVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIKQ 237

Query: 73  ISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYSS 132
           ISIGSYVEDKVPSLKHFMIEENDGYKH IPSLAALVRSKVLLQRHEFKINHGEWVRSYSS
Sbjct: 238 ISIGSYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKVLLQRHEFKINHGEWVRSYSS 297

Query: 133 HLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLRT 192
           HLGPGKSERISEYIRVATDENTDLS SVQIELRVALAALLEDFGVLVIPTVPGPPPKL T
Sbjct: 298 HLGPGKSERISEYIRVATDENTDLSHSVQIELRVALAALLEDFGVLVIPTVPGPPPKLNT 357

Query: 193 DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL 252
           DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL
Sbjct: 358 DISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDTL 417

Query: 253 YSTLKQEVEASY 264
           YSTLKQEVEASY
Sbjct: 418 YSTLKQEVEASY 429

[Close]

BLAST of Csor.00g292860 vs. ExPASy TrEMBL
Match: A0A6J1HYY1 (amidase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1)

HSP 1 Score: 479 bits (1234), Expect = 1.30e-167
Identity = 247/254 (97.24%), Postives = 248/254 (97.64%), Query Frame = 0


Query: 13  AMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG--DHLI 72
           A+ KRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFG  DHLI
Sbjct: 178 AVFKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGAADHLI 237

Query: 73  KQISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSY 132
           KQISIGSYVEDKVPSLKHFMIEENDGYKH IPSLAALVRSKVLLQRHEFKINHGEWVRSY
Sbjct: 238 KQISIGSYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKVLLQRHEFKINHGEWVRSY 297

Query: 133 SSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKL 192
           SSHLGPGKSERISEYIRVATDENTDLS SVQIELRVALAALLEDFGVLVIPTVPGPPPKL
Sbjct: 298 SSHLGPGKSERISEYIRVATDENTDLSHSVQIELRVALAALLEDFGVLVIPTVPGPPPKL 357

Query: 193 RTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD 252
            TDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD
Sbjct: 358 NTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD 417

Query: 253 TLYSTLKQEVEASY 264
           TLYSTLKQEVEASY
Sbjct: 418 TLYSTLKQEVEASY 431

[Close]

BLAST of Csor.00g292860 vs. ExPASy TrEMBL
Match: A0A1S3BQD5 (amidase 1-like OS=Cucumis melo OX=3656 GN=LOC103492181 PE=3 SV=1)

HSP 1 Score: 423 bits (1087), Expect = 2.73e-145
Identity = 217/253 (85.77%), Postives = 231/253 (91.30%), Query Frame = 0


Query: 13  AMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKL-SSIPSERLTQAFVNSVKKLFGDHLIK 72
           A+LKRVG LLLQEPEVEHYKPT+V IAEDCFKL SSI SERLTQAFV+SV+KLFG HLIK
Sbjct: 178 AILKRVGWLLLQEPEVEHYKPTKVFIAEDCFKLLSSITSERLTQAFVSSVEKLFGGHLIK 237

Query: 73  QISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYS 132
           QIS+G YVEDKVPSLKHFMIEENDGYKH IPSLAALVRSK LLQRHEFKINHGEWV SYS
Sbjct: 238 QISLGKYVEDKVPSLKHFMIEENDGYKHSIPSLAALVRSKRLLQRHEFKINHGEWVGSYS 297

Query: 133 SHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLR 192
           SHLGPG SE+I E+IR ATDEN DLSRS+QIELR  LAALLEDFGVL IPTVPGPPPKL 
Sbjct: 298 SHLGPGISEQILEFIREATDENIDLSRSIQIELREVLAALLEDFGVLAIPTVPGPPPKLN 357

Query: 193 TDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDT 252
           TDISE  DFRAKAFSLL+IA+VSG+CQ +IPLGLYNGLPVSISLLAKHGSDGFLLN+VD 
Sbjct: 358 TDISEQFDFRAKAFSLLTIAAVSGVCQVSIPLGLYNGLPVSISLLAKHGSDGFLLNLVDC 417

Query: 253 LYSTLKQEVEASY 264
           LYSTLK+EVEA Y
Sbjct: 418 LYSTLKEEVEAIY 430

[Close]

BLAST of Csor.00g292860 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 256.5 bits (654), Expect = 2.3e-68
Identity = 135/243 (55.56%), Postives = 176/243 (72.43%), Query Frame = 0


Query: 13  AMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDH-LIK 72
           A LKRVG +LLQ+  +   +P+Q++IA+DCFKL S+P + L Q  V SV+K FG + ++K
Sbjct: 180 ATLKRVGCVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVVK 239

Query: 73  QISIGSYVEDKVPSLKHFMIEEN--DGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRS 132
           ++++G Y+   VPSLKHFM  ++     +  IPSL AL  S  LLQRHEFKINHG W+ S
Sbjct: 240 KVNLGEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISS 299

Query: 133 YSSHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPK 192
                GPG SERI E IR  +DE  D  RSV+ EL  AL+ LL + GVLVIPTVPGPPP 
Sbjct: 300 VKPEFGPGISERIEEAIR-TSDEKIDHCRSVKSELITALSTLLGEKGVLVIPTVPGPPPH 359

Query: 193 LRTDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVV 252
           L+ +++ L  FR++AFSLLSIA VSG CQ +IPLGL+  LPVS+SL+AK+GSDGFLL++V
Sbjct: 360 LQANVAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLV 419

[Close]

BLAST of Csor.00g292860 vs. TAIR 10
Match: AT3G17970.1 (translocon at the outer membrane of chloroplasts 64-III )

HSP 1 Score: 196.1 bits (497), Expect = 3.7e-50
Identity = 103/247 (41.70%), Postives = 152/247 (61.54%), Query Frame = 0


Query: 12  PAMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIK 71
           P  L+RVG +LLQ P      P Q+++A+DCF+L  IP +R+TQ    S +KLFG  L+K
Sbjct: 209 PNTLRRVGHVLLQLPFATQRNPRQIILADDCFQLLKIPVDRITQVVTKSAEKLFGRQLLK 268

Query: 72  QISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYS 131
             ++ +Y E KVPSLK F   +      +  S   L     LLQRHEF  NHG+W+ +  
Sbjct: 269 HQNLETYFETKVPSLKEFARTKAIANTKVSTS-RLLANVMQLLQRHEFLQNHGDWINTVK 328

Query: 132 SHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLR 191
             + P    ++ E   + T+E T+   +++ E RVA+ +LL+D G+LVIPT+P  PPKL 
Sbjct: 329 PAIDPVILSQVCENPEL-TNEETENLNAIRNETRVAIGSLLKDDGILVIPTLPAVPPKLG 388

Query: 192 TDISELHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVDT 251
           +      D++ +A SLLSIAS+SG CQ T+PLG +   P+S+S + +HG D FLL+ V T
Sbjct: 389 SKEITSEDYQNRASSLLSIASISGCCQVTVPLGHHEKCPISVSFIGRHGGDRFLLDTVQT 448

Query: 252 LYSTLKQ 259
           +Y +L++
Sbjct: 449 MYPSLQE 453

[Close]

BLAST of Csor.00g292860 vs. TAIR 10
Match: AT5G09420.1 (translocon at the outer membrane of chloroplasts 64-V )

HSP 1 Score: 165.2 bits (417), Expect = 6.9e-41
Identity = 92/251 (36.65%), Postives = 148/251 (58.96%), Query Frame = 0


Query: 12  PAMLKRVGRLLLQEPEVEHYKPTQVLIAEDCFKLSSIPSERLTQAFVNSVKKLFGDHLIK 71
           P++L +VG  LL    V H +   ++ A+D F+LS IP ++  Q    +++ L G    K
Sbjct: 222 PSVLCQVGHALLNLSAVTHRRQRSLIFADDLFELSDIPKQKSVQVVRKAIENLSGYKTPK 281

Query: 72  QISIGSYVEDKVPSLKHFMIEENDGYKHIIPSLAALVRSKVLLQRHEFKINHGEWVRSYS 131
            +++G YV   VPSL  F  E++   ++   +L AL    + +QRHEFK NH EW ++  
Sbjct: 282 HVNVGQYVASNVPSLAEF-CEQSGKSQNSASTLRALSSVMLAIQRHEFKTNHEEWWQTCK 341

Query: 132 SHLGPGKSERISEYIRVATDENTDLSRSVQIELRVALAALLEDFGVLVIPTVPGPPPKLR 191
           S LGP  S  +   ++   +    L R V+ E+R  + +LL++ G+LVIPTV  PPP+L 
Sbjct: 342 SFLGPRFSNDVVTALKSKNESIKSLYR-VKNEMRATIQSLLKEDGILVIPTVADPPPRLN 401

Query: 192 TDISE-LHDFRAKAFSLLSIASVSGICQATIPLGLYNGLPVSISLLAKHGSDGFLLNVVD 251
           T  ++ L++F  + ++L  IAS+SG CQ TIPLG +   P+S+SLL  +G D FLL+   
Sbjct: 402 TKRNKSLNEFLDRTYALSCIASMSGCCQVTIPLGEHGDRPISVSLLTYYGGDKFLLDTTL 461

Query: 252 TLYSTLKQEVE 262
            +Y++L+ + +
Sbjct: 462 DVYASLQDQAK 470

The following BLAST results are available for this feature:

BLAST of Csor.00g292860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FR37	3.2e-67	55.56	Amidase 1 OS=Arabidopsis thaliana OX=3702 GN=AMI1 PE=1 SV=1	[more]
Q7XTK3	6.3e-55	44.40	Amidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMI1 PE=1 SV=1	[more]
Q9MUK5	5.0e-52	43.60	Translocon at the outer membrane of chloroplasts 64 OS=Pisum sativum OX=3888 GN=...	[more]
Q9LVH5	5.2e-49	41.70	Outer envelope protein 64, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP6...	[more]
F4KCL7	9.8e-40	36.65	Outer envelope protein 64, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OM64...	[more]

BLAST of Csor.00g292860 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6590387.1	7.95e-185	100.00	Amidase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022961361.1	9.73e-172	99.21	amidase 1-like isoform X2 [Cucurbita moschata]	[more]
XP_022961359.1	9.89e-170	98.43	amidase 1-like isoform X1 [Cucurbita moschata]	[more]
XP_022968724.1	2.64e-169	98.02	amidase 1 isoform X2 [Cucurbita maxima]	[more]
XP_022968723.1	2.68e-167	97.24	amidase 1 isoform X1 [Cucurbita maxima]	[more]

BLAST of Csor.00g292860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HA58	4.71e-172	99.21	amidase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111461951 PE=3 SV=...	[more]
A0A6J1HDT1	4.79e-170	98.43	amidase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111461951 PE=3 SV=...	[more]
A0A6J1I0H5	1.28e-169	98.02	amidase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1	[more]
A0A6J1HYY1	1.30e-167	97.24	amidase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467873 PE=3 SV=1	[more]
A0A1S3BQD5	2.73e-145	85.77	amidase 1-like OS=Cucumis melo OX=3656 GN=LOC103492181 PE=3 SV=1	[more]

BLAST of Csor.00g292860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08980.1	2.3e-68	55.56	amidase 1	[more]
AT3G17970.1	3.7e-50	41.70	translocon at the outer membrane of chloroplasts 64-III	[more]
AT5G09420.1	6.9e-41	36.65	translocon at the outer membrane of chloroplasts 64-V	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023631	Amidase signature domain	PFAM	PF01425	Amidase	coord: 150..246 e-value: 5.0E-7 score: 29.1
IPR036928	Amidase signature (AS) superfamily	GENE3D	3.90.1300.10	Amidase signature (AS) domain	coord: 31..259 e-value: 4.7E-18 score: 67.5
IPR036928	Amidase signature (AS) superfamily	SUPERFAMILY	75304	Amidase signature (AS) enzymes	coord: 101..252
None	No IPR available	PANTHER	PTHR46310	AMIDASE 1	coord: 12..262

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g292860.m01	Csor.00g292860.m01	mRNA