Csor.00g204280.m01 (mRNA) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g204280.m01
TypemRNA
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionProtein kinase domain-containing protein
LocationCsor_Chr06: 5020997 .. 5021881 (-)
Sequence length885
RNA-Seq ExpressionCsor.00g204280.m01
SyntenyCsor.00g204280.m01
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTAGTGTAATGAAAGATTCATCAGAGAAGGACTCGGAGGCGTTTGTAGAAGTGAATCCAACCGGTCGATATGGGCGATACAGCGAGCTTCTCGGCTCGGGTGCGGTGAAGAAAGTATATAGAGGATTTGATCAAGAAGAAGGAATTGAGGTAGCTTGGAACCAAGTGAAACTAAGAAGCTTTTCAAATGATCCATCAATGATTGATAGGTTATACTCAGAAGTGACATTGTTAAGAACCTTAAAGAACAACAACATAATTGCCTTGTACGATGTATGGCTCGACAAAGCTCATGGAACACTAAATTTCATTACCGAGGTTTGTACAAGTGGGAACCTTAGAGAGTATAGGAAGAAGCACAGGCAAGTGTCTTTGAAGGCTTTGAAGAAATGGTCCAAGCAAATTCTCAAGGGTTTGCACTATTTGCATACTCGTGAGCCTTGTGTTATCCATAGGGACTTGAATTGTAGCAACCTCTTTGTCAATGGCAATGTCGGGCAGGTGAAAATTGGCGACTTGGGCTTGGCTGCAACGGTGAGAAAGAATCACTCAGCTCATTCAGTGCTTGGGACGCCAGAGTTCATGGCACCTGAGCTATATGAAGAGCATTACACTGAGCTTGTGGATATATATTCATTTGGGATGTGTTTGCTTGAATTGGTCACTTTGGAAATCCCTTATAGTGAATGTGACAATGTTGCCAAAATCTACAAGAAAGTGTCCTCCGGTATTAAGCCACTGGCCCTTGCCAAGGTCAAGAACCTTGAGGTCAAGGCTTTCATAGATAAGTGTCTTGCTCAATCTCAAGCTCGGCCCTCCGCTGCCGACCTCCTCCTCGACCCCTTCTTCAGAGAAATTAACGACGAACACGATGATGATTAG

mRNA sequence

ATGCCTAGTGTAATGAAAGATTCATCAGAGAAGGACTCGGAGGCGTTTGTAGAAGTGAATCCAACCGGTCGATATGGGCGATACAGCGAGCTTCTCGGCTCGGGTGCGGTGAAGAAAGTATATAGAGGATTTGATCAAGAAGAAGGAATTGAGGTAGCTTGGAACCAAGTGAAACTAAGAAGCTTTTCAAATGATCCATCAATGATTGATAGGTTATACTCAGAAGTGACATTGTTAAGAACCTTAAAGAACAACAACATAATTGCCTTGTACGATGTATGGCTCGACAAAGCTCATGGAACACTAAATTTCATTACCGAGGTTTGTACAAGTGGGAACCTTAGAGAGTATAGGAAGAAGCACAGGCAAGTGTCTTTGAAGGCTTTGAAGAAATGGTCCAAGCAAATTCTCAAGGGTTTGCACTATTTGCATACTCGTGAGCCTTGTGTTATCCATAGGGACTTGAATTGTAGCAACCTCTTTGTCAATGGCAATGTCGGGCAGGTGAAAATTGGCGACTTGGGCTTGGCTGCAACGGTGAGAAAGAATCACTCAGCTCATTCAGTGCTTGGGACGCCAGAGTTCATGGCACCTGAGCTATATGAAGAGCATTACACTGAGCTTGTGGATATATATTCATTTGGGATGTGTTTGCTTGAATTGGTCACTTTGGAAATCCCTTATAGTGAATGTGACAATGTTGCCAAAATCTACAAGAAAGTGTCCTCCGGTATTAAGCCACTGGCCCTTGCCAAGGTCAAGAACCTTGAGGTCAAGGCTTTCATAGATAAGTGTCTTGCTCAATCTCAAGCTCGGCCCTCCGCTGCCGACCTCCTCCTCGACCCCTTCTTCAGAGAAATTAACGACGAACACGATGATGATTAG

Coding sequence (CDS)

ATGCCTAGTGTAATGAAAGATTCATCAGAGAAGGACTCGGAGGCGTTTGTAGAAGTGAATCCAACCGGTCGATATGGGCGATACAGCGAGCTTCTCGGCTCGGGTGCGGTGAAGAAAGTATATAGAGGATTTGATCAAGAAGAAGGAATTGAGGTAGCTTGGAACCAAGTGAAACTAAGAAGCTTTTCAAATGATCCATCAATGATTGATAGGTTATACTCAGAAGTGACATTGTTAAGAACCTTAAAGAACAACAACATAATTGCCTTGTACGATGTATGGCTCGACAAAGCTCATGGAACACTAAATTTCATTACCGAGGTTTGTACAAGTGGGAACCTTAGAGAGTATAGGAAGAAGCACAGGCAAGTGTCTTTGAAGGCTTTGAAGAAATGGTCCAAGCAAATTCTCAAGGGTTTGCACTATTTGCATACTCGTGAGCCTTGTGTTATCCATAGGGACTTGAATTGTAGCAACCTCTTTGTCAATGGCAATGTCGGGCAGGTGAAAATTGGCGACTTGGGCTTGGCTGCAACGGTGAGAAAGAATCACTCAGCTCATTCAGTGCTTGGGACGCCAGAGTTCATGGCACCTGAGCTATATGAAGAGCATTACACTGAGCTTGTGGATATATATTCATTTGGGATGTGTTTGCTTGAATTGGTCACTTTGGAAATCCCTTATAGTGAATGTGACAATGTTGCCAAAATCTACAAGAAAGTGTCCTCCGGTATTAAGCCACTGGCCCTTGCCAAGGTCAAGAACCTTGAGGTCAAGGCTTTCATAGATAAGTGTCTTGCTCAATCTCAAGCTCGGCCCTCCGCTGCCGACCTCCTCCTCGACCCCTTCTTCAGAGAAATTAACGACGAACACGATGATGATTAG

Protein sequence

MPSVMKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD
Homology
BLAST of Csor.00g204280.m01 vs. ExPASy Swiss-Prot
Match: Q6ICW6 (Probable serine/threonine-protein kinase WNK11 OS=Arabidopsis thaliana OX=3702 GN=WNK11 PE=2 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 2.3e-130
Identity = 223/289 (77.16%), Postives = 257/289 (88.93%), Query Frame = 0

Query: 6   KDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSND 65
           +   +KDSE+FVEV+PTGRYGRY ELLGSGAVKKVYR FDQEEGIEVAWNQVKLR FS+D
Sbjct: 11  ESEKDKDSESFVEVDPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRCFSDD 70

Query: 66  PSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQVS 125
           P+M +RLYSEV LL+ LKN+NII LY VW D+ + TLNFITE+CTSGNLREYRKKHR VS
Sbjct: 71  PAMTERLYSEVRLLKNLKNSNIITLYKVWRDERNNTLNFITEICTSGNLREYRKKHRHVS 130

Query: 126 LKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNHS 185
           ++ALKKWSKQILKGL YLHT +PC+IHRDLNCSN+FVNGN+GQVKIGDLGLAA V KNH 
Sbjct: 131 MRALKKWSKQILKGLDYLHTHDPCIIHRDLNCSNIFVNGNIGQVKIGDLGLAAIVGKNHL 190

Query: 186 AHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGI 245
           AHS+LGTPEFMAPELYEE+YTE+VDIYS+GMC+LELV+LEIPYSECD+VAKIYK+VS G+
Sbjct: 191 AHSILGTPEFMAPELYEENYTEMVDIYSYGMCVLELVSLEIPYSECDSVAKIYKRVSKGL 250

Query: 246 KPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 295
           KP AL KV + E KAFI+KC+AQ +ARPSAA+LL DPFF  I D+ D+D
Sbjct: 251 KPEALNKVNDPEAKAFIEKCIAQPRARPSAAELLCDPFFDGILDDDDED 299

BLAST of Csor.00g204280.m01 vs. ExPASy Swiss-Prot
Match: Q0D541 (Probable serine/threonine-protein kinase WNK5 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK5 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 6.5e-109
Identity = 183/284 (64.44%), Postives = 241/284 (84.86%), Query Frame = 0

Query: 8   SSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN-DP 67
           S+ ++ EAF EV+PTGR+GRY+++LG G+VKKVYRGFDQEEGIEVAWN+V+LR+ ++ DP
Sbjct: 37  SAGEEEEAFEEVDPTGRFGRYADVLGLGSVKKVYRGFDQEEGIEVAWNRVRLRALADRDP 96

Query: 68  SMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQVSL 127
           +M++RL++EV LLR+L + +II  + VWLD+  G LNFITEVCTSG+LREYR +HR VS+
Sbjct: 97  AMVERLHAEVRLLRSLHHEHIIGFHKVWLDRDAGVLNFITEVCTSGSLREYRDRHRHVSV 156

Query: 128 KALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNHSA 187
           KALKKW++QIL GL +LHT +PC+IHRDLNCSN+F+NGN GQVKIGDLGLAA V K H A
Sbjct: 157 KALKKWARQILLGLDHLHTHDPCIIHRDLNCSNVFINGNTGQVKIGDLGLAAIVDKTHVA 216

Query: 188 HSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIK 247
           H++LGTPEFMAPELY E YTE VDIYS+GMC+LE+VT E+PY+ECD+V +IY  V+ G+ 
Sbjct: 217 HTILGTPEFMAPELYTETYTESVDIYSYGMCVLEMVTREMPYAECDSVVQIYHSVTRGVP 276

Query: 248 PLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDE 291
           P AL ++++ E++AFI++C+ Q + RPSAA+LL DPFF  I+D+
Sbjct: 277 PAALKRIRDPELRAFIERCIGQPRNRPSAAELLRDPFFAGIDDD 320

BLAST of Csor.00g204280.m01 vs. ExPASy Swiss-Prot
Match: Q9STK6 (Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana OX=3702 GN=WNK3 PE=2 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.2e-101
Identity = 181/281 (64.41%), Postives = 217/281 (77.22%), Query Frame = 0

Query: 5   MKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN 64
           M+       E FVE++PTGRYGRY E+LG GA K+VYR FDQ EGIEVAWNQVKL     
Sbjct: 1   MRQDENNSEEEFVEIDPTGRYGRYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFC 60

Query: 65  DPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQV 124
               +DRLYSEV LL+TLK+ +II  Y  W+D  H T+N ITEV TSGNLR+YRKKH+ V
Sbjct: 61  SSEDLDRLYSEVHLLKTLKHKSIIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCV 120

Query: 125 SLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNH 184
            L+ALKKWS+QIL+GL YLH+ +P VIHRDL C N+F+NGN G+VKIGDLGLAA + +  
Sbjct: 121 DLRALKKWSRQILEGLVYLHSHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRAR 180

Query: 185 SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG 244
           SAHSV+GTPEFMAPELYEE Y  LVDIY+FGMCLLELVT E PYSEC N A+IY+KV+SG
Sbjct: 181 SAHSVIGTPEFMAPELYEEDYNVLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSG 240

Query: 245 IKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFR 286
           IKP AL  V + +V+AFI+KC+A+   R SA +LL DPF +
Sbjct: 241 IKPAALLNVTDPQVRAFIEKCIAKVSQRLSAKELLDDPFLK 281

BLAST of Csor.00g204280.m01 vs. ExPASy Swiss-Prot
Match: Q9CAV6 (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=1 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.2e-99
Identity = 178/283 (62.90%), Postives = 220/283 (77.74%), Query Frame = 0

Query: 10  EKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSNDPSMI 69
           E D   FVEV+PTGRYGRY+E+LG GA K VYR FD+ EGIEVAWNQVKL  F   P  +
Sbjct: 8   EPDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDL 67

Query: 70  DRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQVSLKAL 129
           +RLY E+ LL+TLK+ NI+  Y  W+D A+  +NF+TE+ TSG LR+YR +H++V+++A+
Sbjct: 68  ERLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAM 127

Query: 130 KKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNHSAHSV 189
           K W +QIL+GLHYLH+ +P VIHRDL C N+FVNGN G+VKIGDLGLAA +RK+H+AH V
Sbjct: 128 KHWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCV 187

Query: 190 LGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPLA 249
            GTPEFMAPE+YEE Y ELVDIYSFGMC+LE+VT + PYSEC + A+IYKKV SG KP A
Sbjct: 188 -GTPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDA 247

Query: 250 LAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHD 293
           L KVK+ EVK FI+KCLA    R SA +LL DPF R  + E D
Sbjct: 248 LYKVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFD 289

BLAST of Csor.00g204280.m01 vs. ExPASy Swiss-Prot
Match: Q65X23 (Probable serine/threonine-protein kinase WNK2 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK2 PE=2 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 6.7e-98
Identity = 170/271 (62.73%), Postives = 213/271 (78.60%), Query Frame = 0

Query: 16  FVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSNDPSMIDRLYSE 75
           F EV+PT RYGRY+E+LG GA K VY+ FDQ EG+EVAWNQ+K+     +   ++RL SE
Sbjct: 18  FAEVDPTARYGRYTEVLGKGAFKTVYKAFDQLEGLEVAWNQIKVGDILRNNDDLERLRSE 77

Query: 76  VTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQVSLKALKKWSKQ 135
           V LL+TLK+ NII  Y+ WLDK +  +NFITEV TSG LR+YR KH++V ++ALKKWS+Q
Sbjct: 78  VRLLKTLKHKNIIKFYNSWLDKKNNNINFITEVFTSGTLRQYRIKHKKVDVRALKKWSRQ 137

Query: 136 ILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNHSAHSVLGTPEF 195
           IL GL YLH+ +P VIHRDL C N+FVNGN G+VKIGDLGLA  +    SAHS++GTPEF
Sbjct: 138 ILSGLVYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLATILDNARSAHSIIGTPEF 197

Query: 196 MAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPLALAKVKN 255
           MAPELY+E Y ELVDIY+FGMCLLELVT E PY EC N A+IYKKVS G KP +LAK+++
Sbjct: 198 MAPELYDEEYNELVDIYAFGMCLLELVTFEYPYCECSNAAQIYKKVSDGEKPSSLAKIED 257

Query: 256 LEVKAFIDKCLAQSQARPSAADLLLDPFFRE 287
            EV+ FI+KC+A++  R SA +LL+DPF R+
Sbjct: 258 PEVRFFIEKCIAKASQRLSAQELLMDPFLRD 288

BLAST of Csor.00g204280.m01 vs. NCBI nr
Match: KAG6596926.1 (putative serine/threonine-protein kinase WNK11, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028406.1 putative serine/threonine-protein kinase WNK11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 590 bits (1521), Expect = 1.25e-212
Identity = 294/294 (100.00%), Postives = 294/294 (100.00%), Query Frame = 0

Query: 1   MPSVMKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 60
           MPSVMKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR
Sbjct: 1   MPSVMKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 60

Query: 61  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 120
           SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK
Sbjct: 61  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 120

Query: 121 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 180
           HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV
Sbjct: 121 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 180

Query: 181 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 240
           RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK
Sbjct: 181 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 240

Query: 241 VSSGIKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 294
           VSSGIKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD
Sbjct: 241 VSSGIKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 294

BLAST of Csor.00g204280.m01 vs. NCBI nr
Match: XP_022943208.1 (probable serine/threonine-protein kinase WNK11 isoform X1 [Cucurbita moschata] >XP_023005493.1 probable serine/threonine-protein kinase WNK11 isoform X1 [Cucurbita maxima])

HSP 1 Score: 586 bits (1510), Expect = 6.16e-211
Identity = 292/294 (99.32%), Postives = 292/294 (99.32%), Query Frame = 0

Query: 1   MPSVMKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 60
           MPSVMKDSSEKDSEAFVEVNPTGRYGRY ELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR
Sbjct: 2   MPSVMKDSSEKDSEAFVEVNPTGRYGRYGELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 61

Query: 61  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 120
           SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK
Sbjct: 62  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 121

Query: 121 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 180
           HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV
Sbjct: 122 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 181

Query: 181 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 240
           RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK
Sbjct: 182 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 241

Query: 241 VSSGIKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 294
           VSSGIKPLALAKVKN EVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD
Sbjct: 242 VSSGIKPLALAKVKNHEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 295

BLAST of Csor.00g204280.m01 vs. NCBI nr
Match: XP_023520905.1 (probable serine/threonine-protein kinase WNK11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583 bits (1503), Expect = 7.19e-210
Identity = 291/294 (98.98%), Postives = 291/294 (98.98%), Query Frame = 0

Query: 1   MPSVMKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 60
           MPSVMKDSSEKDSEAFVEVNPTGRYGRY ELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR
Sbjct: 2   MPSVMKDSSEKDSEAFVEVNPTGRYGRYGELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 61

Query: 61  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 120
           SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK
Sbjct: 62  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 121

Query: 121 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 180
           HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV
Sbjct: 122 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 181

Query: 181 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 240
           RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK
Sbjct: 182 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 241

Query: 241 VSSGIKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 294
           VSSGIKPLALAKVKN EVKAFIDKCLAQSQARPSAADLLLDPFFREINDEH DD
Sbjct: 242 VSSGIKPLALAKVKNHEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHHDD 295

BLAST of Csor.00g204280.m01 vs. NCBI nr
Match: XP_022943216.1 (probable serine/threonine-protein kinase WNK11 isoform X2 [Cucurbita moschata] >XP_023005494.1 probable serine/threonine-protein kinase WNK11 isoform X2 [Cucurbita maxima])

HSP 1 Score: 578 bits (1490), Expect = 5.73e-208
Identity = 288/290 (99.31%), Postives = 288/290 (99.31%), Query Frame = 0

Query: 5   MKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN 64
           MKDSSEKDSEAFVEVNPTGRYGRY ELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN
Sbjct: 1   MKDSSEKDSEAFVEVNPTGRYGRYGELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN 60

Query: 65  DPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQV 124
           DPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQV
Sbjct: 61  DPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQV 120

Query: 125 SLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNH 184
           SLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNH
Sbjct: 121 SLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNH 180

Query: 185 SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG 244
           SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG
Sbjct: 181 SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG 240

Query: 245 IKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 294
           IKPLALAKVKN EVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD
Sbjct: 241 IKPLALAKVKNHEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 290

BLAST of Csor.00g204280.m01 vs. NCBI nr
Match: KAG7015558.1 (putative serine/threonine-protein kinase WNK11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 541 bits (1393), Expect = 4.70e-193
Identity = 270/294 (91.84%), Postives = 277/294 (94.22%), Query Frame = 0

Query: 1   MPSVMKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 60
           MPSV  +SSEKDSE FVEVNPTGRYGRYSELLGSGAVKKVYR FDQEEGIEVAWNQVKLR
Sbjct: 1   MPSVTTESSEKDSEPFVEVNPTGRYGRYSELLGSGAVKKVYRAFDQEEGIEVAWNQVKLR 60

Query: 61  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 120
           SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDK HGTLNFITEVCTSGNLREYRKK
Sbjct: 61  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKK 120

Query: 121 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 180
           HRQVSLKALKKW KQILKGLHYLHT EPCVIHRDLNCSNLFVNGNVGQVKIGDLG+AATV
Sbjct: 121 HRQVSLKALKKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLGMAATV 180

Query: 181 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 240
            KNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK
Sbjct: 181 GKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 240

Query: 241 VSSGIKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 294
           V SGIKPLAL KVK+LEVKAFI+ CLA SQ RPSAADLL  PFF EI+D+ D+D
Sbjct: 241 VCSGIKPLALDKVKDLEVKAFIENCLAPSQDRPSAADLLRHPFFSEIDDDDDED 294

BLAST of Csor.00g204280.m01 vs. ExPASy TrEMBL
Match: A0A6J1L2B5 (probable serine/threonine-protein kinase WNK11 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498462 PE=4 SV=1)

HSP 1 Score: 586 bits (1510), Expect = 2.98e-211
Identity = 292/294 (99.32%), Postives = 292/294 (99.32%), Query Frame = 0

Query: 1   MPSVMKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 60
           MPSVMKDSSEKDSEAFVEVNPTGRYGRY ELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR
Sbjct: 2   MPSVMKDSSEKDSEAFVEVNPTGRYGRYGELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 61

Query: 61  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 120
           SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK
Sbjct: 62  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 121

Query: 121 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 180
           HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV
Sbjct: 122 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 181

Query: 181 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 240
           RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK
Sbjct: 182 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 241

Query: 241 VSSGIKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 294
           VSSGIKPLALAKVKN EVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD
Sbjct: 242 VSSGIKPLALAKVKNHEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 295

BLAST of Csor.00g204280.m01 vs. ExPASy TrEMBL
Match: A0A6J1FWJ3 (probable serine/threonine-protein kinase WNK11 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448004 PE=4 SV=1)

HSP 1 Score: 586 bits (1510), Expect = 2.98e-211
Identity = 292/294 (99.32%), Postives = 292/294 (99.32%), Query Frame = 0

Query: 1   MPSVMKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 60
           MPSVMKDSSEKDSEAFVEVNPTGRYGRY ELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR
Sbjct: 2   MPSVMKDSSEKDSEAFVEVNPTGRYGRYGELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 61

Query: 61  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 120
           SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK
Sbjct: 62  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 121

Query: 121 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 180
           HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV
Sbjct: 122 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 181

Query: 181 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 240
           RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK
Sbjct: 182 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 241

Query: 241 VSSGIKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 294
           VSSGIKPLALAKVKN EVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD
Sbjct: 242 VSSGIKPLALAKVKNHEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 295

BLAST of Csor.00g204280.m01 vs. ExPASy TrEMBL
Match: A0A6J1KZC1 (probable serine/threonine-protein kinase WNK11 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498462 PE=4 SV=1)

HSP 1 Score: 578 bits (1490), Expect = 2.77e-208
Identity = 288/290 (99.31%), Postives = 288/290 (99.31%), Query Frame = 0

Query: 5   MKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN 64
           MKDSSEKDSEAFVEVNPTGRYGRY ELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN
Sbjct: 1   MKDSSEKDSEAFVEVNPTGRYGRYGELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN 60

Query: 65  DPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQV 124
           DPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQV
Sbjct: 61  DPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQV 120

Query: 125 SLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNH 184
           SLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNH
Sbjct: 121 SLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNH 180

Query: 185 SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG 244
           SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG
Sbjct: 181 SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG 240

Query: 245 IKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 294
           IKPLALAKVKN EVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD
Sbjct: 241 IKPLALAKVKNHEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 290

BLAST of Csor.00g204280.m01 vs. ExPASy TrEMBL
Match: A0A6J1FR46 (probable serine/threonine-protein kinase WNK11 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448004 PE=4 SV=1)

HSP 1 Score: 578 bits (1490), Expect = 2.77e-208
Identity = 288/290 (99.31%), Postives = 288/290 (99.31%), Query Frame = 0

Query: 5   MKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN 64
           MKDSSEKDSEAFVEVNPTGRYGRY ELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN
Sbjct: 1   MKDSSEKDSEAFVEVNPTGRYGRYGELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN 60

Query: 65  DPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQV 124
           DPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQV
Sbjct: 61  DPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQV 120

Query: 125 SLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNH 184
           SLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNH
Sbjct: 121 SLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNH 180

Query: 185 SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG 244
           SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG
Sbjct: 181 SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG 240

Query: 245 IKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 294
           IKPLALAKVKN EVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD
Sbjct: 241 IKPLALAKVKNHEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 290

BLAST of Csor.00g204280.m01 vs. ExPASy TrEMBL
Match: A0A6J1HMV6 (probable serine/threonine-protein kinase WNK11 OS=Cucurbita maxima OX=3661 GN=LOC111465082 PE=4 SV=1)

HSP 1 Score: 538 bits (1385), Expect = 4.06e-192
Identity = 268/294 (91.16%), Postives = 276/294 (93.88%), Query Frame = 0

Query: 1   MPSVMKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLR 60
           MPSV  +SSEKDSE FVEVNPTGRYGRY ELLGSGAVKKVYR FDQEEGIEVAWNQVKLR
Sbjct: 2   MPSVTTESSEKDSEPFVEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLR 61

Query: 61  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKK 120
           SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDK HGTLNFITEVCTSGNLREYRKK
Sbjct: 62  SFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKK 121

Query: 121 HRQVSLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATV 180
           HRQVSLKALKKW KQILKGLHYLHT EPCVIHRDLNCSNLFVNGNVGQVKIGDLG+AATV
Sbjct: 122 HRQVSLKALKKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLGMAATV 181

Query: 181 RKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 240
            KNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK
Sbjct: 182 GKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKK 241

Query: 241 VSSGIKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 294
           V SGIKPLAL KVK+LEVKAFI+ CLA  Q RPSAADLL  PFF EI+D++DD+
Sbjct: 242 VCSGIKPLALDKVKDLEVKAFIENCLAPFQDRPSAADLLRHPFFSEIDDDNDDE 295

BLAST of Csor.00g204280.m01 vs. TAIR 10
Match: AT5G55560.1 (Protein kinase superfamily protein )

HSP 1 Score: 466.5 bits (1199), Expect = 1.6e-131
Identity = 223/289 (77.16%), Postives = 257/289 (88.93%), Query Frame = 0

Query: 6   KDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSND 65
           +   +KDSE+FVEV+PTGRYGRY ELLGSGAVKKVYR FDQEEGIEVAWNQVKLR FS+D
Sbjct: 11  ESEKDKDSESFVEVDPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRCFSDD 70

Query: 66  PSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQVS 125
           P+M +RLYSEV LL+ LKN+NII LY VW D+ + TLNFITE+CTSGNLREYRKKHR VS
Sbjct: 71  PAMTERLYSEVRLLKNLKNSNIITLYKVWRDERNNTLNFITEICTSGNLREYRKKHRHVS 130

Query: 126 LKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNHS 185
           ++ALKKWSKQILKGL YLHT +PC+IHRDLNCSN+FVNGN+GQVKIGDLGLAA V KNH 
Sbjct: 131 MRALKKWSKQILKGLDYLHTHDPCIIHRDLNCSNIFVNGNIGQVKIGDLGLAAIVGKNHL 190

Query: 186 AHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGI 245
           AHS+LGTPEFMAPELYEE+YTE+VDIYS+GMC+LELV+LEIPYSECD+VAKIYK+VS G+
Sbjct: 191 AHSILGTPEFMAPELYEENYTEMVDIYSYGMCVLELVSLEIPYSECDSVAKIYKRVSKGL 250

Query: 246 KPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHDDD 295
           KP AL KV + E KAFI+KC+AQ +ARPSAA+LL DPFF  I D+ D+D
Sbjct: 251 KPEALNKVNDPEAKAFIEKCIAQPRARPSAAELLCDPFFDGILDDDDED 299

BLAST of Csor.00g204280.m01 vs. TAIR 10
Match: AT3G48260.1 (with no lysine (K) kinase 3 )

HSP 1 Score: 370.2 bits (949), Expect = 1.6e-102
Identity = 181/281 (64.41%), Postives = 217/281 (77.22%), Query Frame = 0

Query: 5   MKDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSN 64
           M+       E FVE++PTGRYGRY E+LG GA K+VYR FDQ EGIEVAWNQVKL     
Sbjct: 1   MRQDENNSEEEFVEIDPTGRYGRYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFC 60

Query: 65  DPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQV 124
               +DRLYSEV LL+TLK+ +II  Y  W+D  H T+N ITEV TSGNLR+YRKKH+ V
Sbjct: 61  SSEDLDRLYSEVHLLKTLKHKSIIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCV 120

Query: 125 SLKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNH 184
            L+ALKKWS+QIL+GL YLH+ +P VIHRDL C N+F+NGN G+VKIGDLGLAA + +  
Sbjct: 121 DLRALKKWSRQILEGLVYLHSHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRAR 180

Query: 185 SAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSG 244
           SAHSV+GTPEFMAPELYEE Y  LVDIY+FGMCLLELVT E PYSEC N A+IY+KV+SG
Sbjct: 181 SAHSVIGTPEFMAPELYEEDYNVLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSG 240

Query: 245 IKPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFR 286
           IKP AL  V + +V+AFI+KC+A+   R SA +LL DPF +
Sbjct: 241 IKPAALLNVTDPQVRAFIEKCIAKVSQRLSAKELLDDPFLK 281

BLAST of Csor.00g204280.m01 vs. TAIR 10
Match: AT3G04910.1 (with no lysine (K) kinase 1 )

HSP 1 Score: 364.4 bits (934), Expect = 8.7e-101
Identity = 178/283 (62.90%), Postives = 220/283 (77.74%), Query Frame = 0

Query: 10  EKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSNDPSMI 69
           E D   FVEV+PTGRYGRY+E+LG GA K VYR FD+ EGIEVAWNQVKL  F   P  +
Sbjct: 8   EPDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDL 67

Query: 70  DRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQVSLKAL 129
           +RLY E+ LL+TLK+ NI+  Y  W+D A+  +NF+TE+ TSG LR+YR +H++V+++A+
Sbjct: 68  ERLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAM 127

Query: 130 KKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNHSAHSV 189
           K W +QIL+GLHYLH+ +P VIHRDL C N+FVNGN G+VKIGDLGLAA +RK+H+AH V
Sbjct: 128 KHWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCV 187

Query: 190 LGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPLA 249
            GTPEFMAPE+YEE Y ELVDIYSFGMC+LE+VT + PYSEC + A+IYKKV SG KP A
Sbjct: 188 -GTPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDA 247

Query: 250 LAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREINDEHD 293
           L KVK+ EVK FI+KCLA    R SA +LL DPF R  + E D
Sbjct: 248 LYKVKDPEVKCFIEKCLATVSLRVSARELLDDPFLRIDDGEFD 289

BLAST of Csor.00g204280.m01 vs. TAIR 10
Match: AT3G18750.1 (with no lysine (K) kinase 6 )

HSP 1 Score: 346.7 bits (888), Expect = 1.9e-95
Identity = 165/269 (61.34%), Postives = 214/269 (79.55%), Query Frame = 0

Query: 17  VEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSNDPSMIDRLYSEV 76
           +EV+PT RY RY E++G GA K VY+ FD+ +GIEVAWNQV++      P+ ++RLYSEV
Sbjct: 19  LEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRIDDVLQSPNCLERLYSEV 78

Query: 77  TLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQVSLKALKKWSKQI 136
            LL++LK+NNII  Y+ W+D  + T+N ITE+ TSG+LR YRKKHR+V++KA+K W++QI
Sbjct: 79  RLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHRKVNMKAVKNWARQI 138

Query: 137 LKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNHSAHSVLGTPEFM 196
           L GL YLH +EP +IHRDL C N+F+NGN G+VKIGDLGL ATV +  +A SV+GTPEFM
Sbjct: 139 LMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGL-ATVMEQANAKSVIGTPEFM 198

Query: 197 APELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGIKPLALAKVKNL 256
           APELY+E+Y EL DIYSFGMC+LE+VT + PY EC N A+IYKKVSSGIKP +L++VK+ 
Sbjct: 199 APELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSSGIKPASLSRVKDP 258

Query: 257 EVKAFIDKCLAQSQARPSAADLLLDPFFR 286
           EVK FI+KCL  +  R SA +LLLDPF +
Sbjct: 259 EVKQFIEKCLLPASERLSAKELLLDPFLQ 286

BLAST of Csor.00g204280.m01 vs. TAIR 10
Match: AT3G22420.1 (with no lysine (K) kinase 2 )

HSP 1 Score: 346.7 bits (888), Expect = 1.9e-95
Identity = 161/283 (56.89%), Postives = 220/283 (77.74%), Query Frame = 0

Query: 6   KDSSEKDSEAFVEVNPTGRYGRYSELLGSGAVKKVYRGFDQEEGIEVAWNQVKLRSFSND 65
           ++S  +D   FVE++P+GRYGRY E+LG GA K VYR FD+ EGIEVAWNQVKLR+F+ +
Sbjct: 4   EESFVEDCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRN 63

Query: 66  PSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKAHGTLNFITEVCTSGNLREYRKKHRQVS 125
           P  +++ + E+ LL+TL + NI+  Y  W+D  + ++NF+TE+ TSG LR+YR +HR+V+
Sbjct: 64  PEELEKFFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVN 123

Query: 126 LKALKKWSKQILKGLHYLHTREPCVIHRDLNCSNLFVNGNVGQVKIGDLGLAATVRKNHS 185
           ++A+K+W KQILKGL YLH+R P +IHRDL C N+F+NGN G+VKIGDLGLAA +RK+H+
Sbjct: 124 IRAVKQWCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHA 183

Query: 186 AHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVSSGI 245
              V GTPEFMAPE+Y+E Y ELVD+Y+FGMC+LE+VT + PYSEC + A+IYKKV+SG 
Sbjct: 184 VRCV-GTPEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGK 243

Query: 246 KPLALAKVKNLEVKAFIDKCLAQSQARPSAADLLLDPFFREIN 289
           KP A   VK+ EV+ F++KCLA    R +A +LL DPF ++ N
Sbjct: 244 KPEAFYLVKDPEVREFVEKCLANVTCRLTALELLQDPFLQDDN 285

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6ICW62.3e-13077.16Probable serine/threonine-protein kinase WNK11 OS=Arabidopsis thaliana OX=3702 G... [more]
Q0D5416.5e-10964.44Probable serine/threonine-protein kinase WNK5 OS=Oryza sativa subsp. japonica OX... [more]
Q9STK62.2e-10164.41Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9CAV61.2e-9962.90Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=... [more]
Q65X236.7e-9862.73Probable serine/threonine-protein kinase WNK2 OS=Oryza sativa subsp. japonica OX... [more]
Match NameE-valueIdentityDescription
KAG6596926.11.25e-212100.00putative serine/threonine-protein kinase WNK11, partial [Cucurbita argyrosperma ... [more]
XP_022943208.16.16e-21199.32probable serine/threonine-protein kinase WNK11 isoform X1 [Cucurbita moschata] >... [more]
XP_023520905.17.19e-21098.98probable serine/threonine-protein kinase WNK11 [Cucurbita pepo subsp. pepo][more]
XP_022943216.15.73e-20899.31probable serine/threonine-protein kinase WNK11 isoform X2 [Cucurbita moschata] >... [more]
KAG7015558.14.70e-19391.84putative serine/threonine-protein kinase WNK11, partial [Cucurbita argyrosperma ... [more]
Match NameE-valueIdentityDescription
A0A6J1L2B52.98e-21199.32probable serine/threonine-protein kinase WNK11 isoform X1 OS=Cucurbita maxima OX... [more]
A0A6J1FWJ32.98e-21199.32probable serine/threonine-protein kinase WNK11 isoform X1 OS=Cucurbita moschata ... [more]
A0A6J1KZC12.77e-20899.31probable serine/threonine-protein kinase WNK11 isoform X2 OS=Cucurbita maxima OX... [more]
A0A6J1FR462.77e-20899.31probable serine/threonine-protein kinase WNK11 isoform X2 OS=Cucurbita moschata ... [more]
A0A6J1HMV64.06e-19291.16probable serine/threonine-protein kinase WNK11 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G55560.11.6e-13177.16Protein kinase superfamily protein [more]
AT3G48260.11.6e-10264.41with no lysine (K) kinase 3 [more]
AT3G04910.18.7e-10162.90with no lysine (K) kinase 1 [more]
AT3G18750.11.9e-9561.34with no lysine (K) kinase 6 [more]
AT3G22420.11.9e-9556.89with no lysine (K) kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePIRSFPIRSF000615TyrPK_CSF1-Rcoord: 26..128
e-value: 1.7
score: 4.6
coord: 119..280
e-value: 2.6E-13
score: 46.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 109..290
e-value: 1.5E-47
score: 163.4
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 9..107
e-value: 5.4E-29
score: 102.1
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 27..239
e-value: 2.9E-26
score: 90.0
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 31..275
e-value: 4.9E-14
score: 49.8
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 1..293
NoneNo IPR availablePANTHERPTHR13902:SF119SERINE/THREONINE-KINASE WNK11-LIKE PROTEINcoord: 1..293
NoneNo IPR availableCDDcd13983STKc_WNKcoord: 24..284
e-value: 7.20444E-153
score: 425.871
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 29..284
e-value: 7.1E-50
score: 169.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 26..284
score: 39.22681
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 28..290

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Csor.00g204280Csor.00g204280gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g204280.m01.CDS01Csor.00g204280.m01.CDS01CDS


The following single feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g204280.m01.single01Csor.00g204280.m01.single01single


The following stop_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g204280.m01.stop_codon01Csor.00g204280.m01.stop_codon01stop_codon


The following start_codon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Csor.00g204280.m01.start_codon01Csor.00g204280.m01.start_codon01start_codon


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Csor.00g204280.m01Csor.00g204280.m01-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0035556 intracellular signal transduction
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity