Csor.00g196100 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g196100
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	Csor_Chr01: 1129463 .. 1130654 (+)
RNA-Seq Expression	Csor.00g196100
Synteny	Csor.00g196100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSinitialstart_codonpolypeptideintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGATGAGATTGGGAAGTTGTTGAAAGTGTGGATTTCAATATGGATGTGTTTGGGGTATTGCTACGGCGTTGGCAAGAAAATTGGAGAGGGATTTACAAGATTTCTCTTAATTTTCCCTGTCATTTGCATCTTCCTCTACCTTCCCCTCAATCTCCACTCCCTCCATCTCGGCGGTCTCACCGCGTTTTTCGTTGCTTGGCTCGCTAATTTCAAGCTCCTCCTCTTCGCCGCCGGTCAAGGCCCTCTGGCCACCACCGGGATGTCTATCGGTATGTTTAATCATTTCTTCATCTTCTTAATTCTGTTTCTTAAATTCCCACCAAACTCAAGAAATTCTACTCTTTCCGACAGCTGAATTCTGCGCAGTGGCTTCCTTCCCCATCAAAATTAAGCAAATCCCGTCTCAAAAAAGCTCAGAGCCAGCCAATAATGGTGGAGCAGCCACCGTAATTGATCCAAAATCCAACCAGAATCACCGCGAATCGCCATTTAACTACGCCGTCAAGATCTTGCTTGTGGCGGCGCTAGTCAAGGCCTATGACTACACCCACATTCTCCCTCAGAAGCTCGTCTGGTTCCTCCTCTGTTTCCACATCTACTTCATCCTCGACCTCAGCTTCGCCGCCGCCTCTGCCGCCGTCTCCCACGCCGGTACCATGGAGCTCCTCCCCCTCTTTCATCATCCCTACCTCTCCACTTCCCTTCAAGACTTCTGGGGACGCCGATGGAACCTCGCCGTCACCGGAATCCTCCGTCCCGTCGCATACCGCCCTGCCGTCTCTACCGCAACGAAACTCATCGGTCCCCGGTGGGCCCCACTCGCCGGAGTGGCGGTGACATTTCTGGTGTCGGCGGCGATGCACGAGTTGATGTTCTTCTACATGTCGCGACAGTGGCCGCCACCGACGTGGGAGGTGGCGGCGTTCTTCGTCCTGCACGGTGGGTGTCTGGCGGTGGAAATGGTGGCGAAGAGGCTGTGGATCGATGCGTGGAAAATGCCGCCGCTACCGGCCGCGGTGTCAGTGCCCGTGACGGTGGGATTCTTCTTCTGGACGTGCTTTTGGCTGTTCTTTCCGCCGTTCATCAACACGTGTAAGGCTGACGTCAGGGTGGTTAAGGAGTACGAGATATTTGGCGCGTTTCTGAAGAATAATATTATTCCAACTACATTTTTTCCCTCCAAATGA

mRNA sequence

ATGGAGGATGAGATTGGGAAGTTGTTGAAAGTGTGGATTTCAATATGGATGTGTTTGGGGTATTGCTACGGCGTTGGCAAGAAAATTGGAGAGGGATTTACAAGATTTCTCTTAATTTTCCCTGTCATTTGCATCTTCCTCTACCTTCCCCTCAATCTCCACTCCCTCCATCTCGGCGGTCTCACCGCGTTTTTCGTTGCTTGGCTCGCTAATTTCAAGCTCCTCCTCTTCGCCGCCGGTCAAGGCCCTCTGGCCACCACCGGGATGTCTATCGTGGCTTCCTTCCCCATCAAAATTAAGCAAATCCCGTCTCAAAAAAGCTCAGAGCCAGCCAATAATGGTGGAGCAGCCACCGTAATTGATCCAAAATCCAACCAGAATCACCGCGAATCGCCATTTAACTACGCCGTCAAGATCTTGCTTGTGGCGGCGCTAGTCAAGGCCTATGACTACACCCACATTCTCCCTCAGAAGCTCGTCTGGTTCCTCCTCTGTTTCCACATCTACTTCATCCTCGACCTCAGCTTCGCCGCCGCCTCTGCCGCCGTCTCCCACGCCGGTACCATGGAGCTCCTCCCCCTCTTTCATCATCCCTACCTCTCCACTTCCCTTCAAGACTTCTGGGGACGCCGATGGAACCTCGCCGTCACCGGAATCCTCCGTCCCGTCGCATACCGCCCTGCCGTCTCTACCGCAACGAAACTCATCGGTCCCCGGTGGGCCCCACTCGCCGGAGTGGCGGTGACATTTCTGGTGTCGGCGGCGATGCACGAGTTGATGTTCTTCTACATGTCGCGACAGTGGCCGCCACCGACGTGGGAGGTGGCGGCGTTCTTCGTCCTGCACGGTGGGTGTCTGGCGGTGGAAATGGTGGCGAAGAGGCTGTGGATCGATGCGTGGAAAATGCCGCCGCTACCGGCCGCGGTGTCAGTGCCCGTGACGGTGGGATTCTTCTTCTGGACGTGCTTTTGGCTGTTCTTTCCGCCGTTCATCAACACGTGTAAGGCTGACGTCAGGGTGGTTAAGGAGTACGAGATATTTGGCGCGTTTCTGAAGAATAATATTATTCCAACTACATTTTTTCCCTCCAAATGA

Coding sequence (CDS)

ATGGAGGATGAGATTGGGAAGTTGTTGAAAGTGTGGATTTCAATATGGATGTGTTTGGGGTATTGCTACGGCGTTGGCAAGAAAATTGGAGAGGGATTTACAAGATTTCTCTTAATTTTCCCTGTCATTTGCATCTTCCTCTACCTTCCCCTCAATCTCCACTCCCTCCATCTCGGCGGTCTCACCGCGTTTTTCGTTGCTTGGCTCGCTAATTTCAAGCTCCTCCTCTTCGCCGCCGGTCAAGGCCCTCTGGCCACCACCGGGATGTCTATCGTGGCTTCCTTCCCCATCAAAATTAAGCAAATCCCGTCTCAAAAAAGCTCAGAGCCAGCCAATAATGGTGGAGCAGCCACCGTAATTGATCCAAAATCCAACCAGAATCACCGCGAATCGCCATTTAACTACGCCGTCAAGATCTTGCTTGTGGCGGCGCTAGTCAAGGCCTATGACTACACCCACATTCTCCCTCAGAAGCTCGTCTGGTTCCTCCTCTGTTTCCACATCTACTTCATCCTCGACCTCAGCTTCGCCGCCGCCTCTGCCGCCGTCTCCCACGCCGGTACCATGGAGCTCCTCCCCCTCTTTCATCATCCCTACCTCTCCACTTCCCTTCAAGACTTCTGGGGACGCCGATGGAACCTCGCCGTCACCGGAATCCTCCGTCCCGTCGCATACCGCCCTGCCGTCTCTACCGCAACGAAACTCATCGGTCCCCGGTGGGCCCCACTCGCCGGAGTGGCGGTGACATTTCTGGTGTCGGCGGCGATGCACGAGTTGATGTTCTTCTACATGTCGCGACAGTGGCCGCCACCGACGTGGGAGGTGGCGGCGTTCTTCGTCCTGCACGGTGGGTGTCTGGCGGTGGAAATGGTGGCGAAGAGGCTGTGGATCGATGCGTGGAAAATGCCGCCGCTACCGGCCGCGGTGTCAGTGCCCGTGACGGTGGGATTCTTCTTCTGGACGTGCTTTTGGCTGTTCTTTCCGCCGTTCATCAACACGTGTAAGGCTGACGTCAGGGTGGTTAAGGAGTACGAGATATTTGGCGCGTTTCTGAAGAATAATATTATTCCAACTACATTTTTTCCCTCCAAATGA

Protein sequence

MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGGLTAFFVAWLANFKLLLFAAGQGPLATTGMSIVASFPIKIKQIPSQKSSEPANNGGAATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLSFAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKLIGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKNNIIPTTFFPSK

Homology

[Close]

BLAST of Csor.00g196100 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 9.9e-67
Identity = 142/353 (40.23%), Postives = 202/353 (57.22%), Query Frame = 0


Query: 5   IGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGGLTAF 64
           +   +K W  + + L Y + + K + +G  R +L FPV  IF  +P  ++SLHL G+TAF
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 65  FVAWLANFKLLLFAAGQGPLATTGMSI-------VASFPIKIKQIPSQKSSEPANNGGAA 124
           F+AWLANFKLLLFA G+GPL++    +       V+  PIKI+  P    +         
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTH-------- 120

Query: 125 TVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLSFA 184
                 S++   E P  Y +K + V  ++KAY+Y+  LP+K+V  L   HIYF L++  A
Sbjct: 121 ------SHEGSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILA 180

Query: 185 AASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKLIG 244
           A +AAV     +EL P F+ PYL+TSLQDFWGRRWNL VTGILRP  Y P++   + ++G
Sbjct: 181 ATAAAVRAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLG 240

Query: 245 PRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRLWI 304
           P ++ +     TF+VS  MHEL+FFYM R    P W++  FF+++G C  VE+  K+   
Sbjct: 241 PNYSQILAAFGTFVVSGIMHELIFFYMGRL--RPDWKMMWFFLINGFCTTVEIAIKKTIN 300

Query: 305 DAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAF 351
             W+    P A+S  +T+ F   T  WLF P F N C    + + EY   GAF
Sbjct: 301 GRWR---FPKAISQVLTLTFVMVTALWLFLPEF-NRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of Csor.00g196100 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.6e-56
Identity = 136/334 (40.72%), Postives = 179/334 (53.59%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           ME++   L++VWIS  + L YCY +  K+ +G  R L I PV  +FL LPL L  +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPL-----ATTGMSIVASFPIKIKQIPSQKSSEPANNGG 120
           ++  F++WLANFKLLLFA  +GPL       +     A  PIKI+Q PS           
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPPKLSRFICFACLPIKIRQDPS----------- 120

Query: 121 AATVIDPKSNQNHRESP---FNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFIL 180
                 P +  N    P   +  AVKIL++  L+  Y+Y   LP+ +V  L C HIY  +
Sbjct: 121 ------PNAIPNLHPKPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEV 180

Query: 181 DLSFAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTA 240
           +L      A VS      + P+F+ PYL+TSLQDFW RRWNL V+ +LR   + P     
Sbjct: 181 ELVLVFVGAVVSTLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFF 240

Query: 241 TKLIGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVA 300
            +++ P  A  AGV  +F VS  MHEL++FYM R+  PPTWEV  FFVLHG   A E+  
Sbjct: 241 KRILSPDGAMFAGVMASFFVSGLMHELLYFYMIRK--PPTWEVTCFFVLHGAATATEIAV 300

Query: 301 KRLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLF 327
           KR     W  PP   AVS  V + F   T  WLF
Sbjct: 301 KR---TQWLRPP-HRAVSGLVVLTFVSVTGVWLF 311

[Close]

BLAST of Csor.00g196100 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.6e-56
Identity = 137/359 (38.16%), Postives = 189/359 (52.65%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           ME+E+  L+KVW+   + + YCY    +I  G  R L + PV  +FL LPL + S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
            TAFF++WLANFKL+LF+  QGPL     ++        FPIK++Q P  ++  P     
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPSNLSRFVCFTCFPIKLQQNPKPQNQMP----- 120

Query: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180
                            + +AVK+     L+  Y+Y   +   ++  L   HIY   ++ 
Sbjct: 121 ----------------KWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEIL 180

Query: 181 FAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240
            A     +S +   +L P F+ PYLSTSLQDFWGRRWNL V  ILRP  Y P    A + 
Sbjct: 181 LAPLKVLLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRK 240

Query: 241 IGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300
           +    A   GV  +FLVS  +HEL+FFY +R+   PT EV  FFVLHG C A E  AKR 
Sbjct: 241 MNSDQALFLGVFASFLVSGVVHELIFFYFTRE--SPTGEVTLFFVLHGVCTAAECAAKRT 300

Query: 301 -WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 354
             +  WK+  +   VS  +TVGF   T  WLFFP    +   + R+  E  +F  F+K+
Sbjct: 301 RLVRRWKVSQM---VSRLLTVGFVVMTGGWLFFPHLARSGMIE-RLADEAFLFIGFVKH 332

[Close]

BLAST of Csor.00g196100 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.0e-55
Identity = 132/335 (39.40%), Postives = 182/335 (54.33%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           M++E+  L+KVW+S  + + YCY +  +I  G  RFL + PV+ +FL LPL   SLHL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
           +TAFF+ WLANFKL+LF+  +GPL     +         FPIK++Q              
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIPTNFPRFFCFTCFPIKVQQ-------------- 120

Query: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180
                +PKS QNH      +A+K+ + A L+  Y Y   L   ++  L   H+Y  +++ 
Sbjct: 121 -----NPKS-QNHLPK-LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEII 180

Query: 181 FAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240
                  V  +   +L P  + PYL+TSLQDFWGRRWNL V  ILRP  Y P    + + 
Sbjct: 181 LTFVKVVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERK 240

Query: 241 IGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300
           +   WA   G+   F+VS  +HEL+FFY+ R+   PT EV  FFVLHG C AVE+  K+ 
Sbjct: 241 MSSGWALFPGILAAFIVSGLVHELLFFYLIREM--PTGEVTLFFVLHGVCTAVELAVKKK 300

Query: 301 WIDA--WKMPPLPAAVSVPVTVGFFFWTCFWLFFP 329
              A  W++ P    VS  +TVGF F T  WLF P
Sbjct: 301 TTVAQRWRLSP---GVSRVLTVGFVFVTGGWLFTP 309

[Close]

BLAST of Csor.00g196100 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 8.7e-55
Identity = 134/363 (36.91%), Postives = 191/363 (52.62%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGE-GFTRFLLIFPVICIFLYLPLNLHSLHLG 60
           +E E+    +VWIS      YC  V       G  R LL+ PV+ +F++LPL L S HLG
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLG 62

Query: 61  GLTAFFVAWLANFKLLLFAAGQGPLATTGMSIVASFPIKIKQIPSQKSSEPANNGGAATV 120
           G TA ++ WLANFKLLLFA   GPL+   +S++    I    +P +   +P+N+      
Sbjct: 63  GPTALYLVWLANFKLLLFAFHLGPLSNPSLSLLHF--ISTTLLPIKFRDDPSND------ 122

Query: 121 IDPKSNQNHRESPFNYAVKILLVAALV------KAYDYTHILPQKLVWFLLCFHIYFILD 180
                 +N R   F +   +L VA LV      K Y++   LP  ++  L CFH Y   +
Sbjct: 123 ----HEKNKRTLSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTE 182

Query: 181 LSFAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTAT 240
           ++ AA++        ++L P F+ PYL+TSLQDFWGRRWNL V+ IL    Y+P     +
Sbjct: 183 ITLAASAVIARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLS 242

Query: 241 KLIGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAK 300
           + +  RW     + V F VS  MHE+ FFY++R    P+WEV  FFVLHG C AVEMV K
Sbjct: 243 RWVRLRWEVAGAMLVAFTVSGLMHEVFFFYLTR--ARPSWEVTGFFVLHGVCTAVEMVVK 302

Query: 301 RLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 357
           +      +   L   VS  +TVGF   T  WLF P  +     D++ + EY +   + + 
Sbjct: 303 KAVSGKVR---LRREVSGALTVGFVMVTGGWLFLPQLVRH-GVDLKTIDEYPVMFNYTQK 347

[Close]

BLAST of Csor.00g196100 vs. NCBI nr
Match: KAG6606896.1 (Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 751 bits (1940), Expect = 3.90e-274
Identity = 364/364 (100.00%), Postives = 364/364 (100.00%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG
Sbjct: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIVASFPIKIKQIPSQKSSEPANNGGAATVI 120
           LTAFFVAWLANFKLLLFAAGQGPLATTGMSIVASFPIKIKQIPSQKSSEPANNGGAATVI
Sbjct: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIVASFPIKIKQIPSQKSSEPANNGGAATVI 120

Query: 121 DPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLSFAAAS 180
           DPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLSFAAAS
Sbjct: 121 DPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLSFAAAS 180

Query: 181 AAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKLIGPRW 240
           AAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKLIGPRW
Sbjct: 181 AAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKLIGPRW 240

Query: 241 APLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRLWIDAW 300
           APLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRLWIDAW
Sbjct: 241 APLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRLWIDAW 300

Query: 301 KMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKNNIIPTTF 360
           KMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKNNIIPTTF
Sbjct: 301 KMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKNNIIPTTF 360

Query: 361 FPSK 364
           FPSK
Sbjct: 361 FPSK 364

[Close]

BLAST of Csor.00g196100 vs. NCBI nr
Match: XP_023523549.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 731 bits (1888), Expect = 3.99e-266
Identity = 356/369 (96.48%), Postives = 358/369 (97.02%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           MEDEIGKLLKVWISIW+CLGYCYGVG KIGEGFTRFL IFPVICIFLYLPLNLHSLHLGG
Sbjct: 1   MEDEIGKLLKVWISIWICLGYCYGVGNKIGEGFTRFLFIFPVICIFLYLPLNLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
           LTAFFVAWLANFKLLLFAAGQGPLATTGMSI     VASFPIKIKQIP QKSSEPA NGG
Sbjct: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQIPPQKSSEPAGNGG 120

Query: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180
           AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS
Sbjct: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180

Query: 181 FAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240
           FAAASAAVSH GT+ELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL
Sbjct: 181 FAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240

Query: 241 IGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300
           IGPRWAPLAGVA TFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL
Sbjct: 241 IGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300

Query: 301 WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKNNI 360
           WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKNNI
Sbjct: 301 WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKNNI 360

Query: 361 IPTTFFPSK 364
           IPTTFFPSK
Sbjct: 361 IPTTFFPSK 369

[Close]

BLAST of Csor.00g196100 vs. NCBI nr
Match: XP_022998457.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 696 bits (1797), Expect = 2.96e-252
Identity = 341/358 (95.25%), Postives = 343/358 (95.81%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           MEDEIG LLKVWISIW+CLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPL LHSLHLGG
Sbjct: 1   MEDEIGNLLKVWISIWICLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLKLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
           LTAFFVAWLANFKLLLFAAGQGPLATTGMSI     VASFPIKIKQI  QKSSEPA NGG
Sbjct: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQILPQKSSEPAGNGG 120

Query: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180
           AATVIDPKSNQNH ESPFNYAVKILLVAALVKAYDYTHIL QKLVWFLLCFHIYFILDLS
Sbjct: 121 AATVIDPKSNQNHCESPFNYAVKILLVAALVKAYDYTHILHQKLVWFLLCFHIYFILDLS 180

Query: 181 FAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240
           FAAASAAVSH GT+ELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL
Sbjct: 181 FAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240

Query: 241 IGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300
           IGPRWAPLAGVA TFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL
Sbjct: 241 IGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300

Query: 301 WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 353
           WIDAWKMPPLPA VSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN
Sbjct: 301 WIDAWKMPPLPAVVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 358

[Close]

BLAST of Csor.00g196100 vs. NCBI nr
Match: KAG7036600.1 (Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 715 bits (1846), Expect = 1.67e-251
Identity = 349/354 (98.59%), Postives = 349/354 (98.59%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG
Sbjct: 167 MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 226

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
           LTAFFVAWLANFKLLLFAAGQGPLATTGMSI     VASFPIKIKQIPSQKSSEPANNGG
Sbjct: 227 LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQIPSQKSSEPANNGG 286

Query: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180
           AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS
Sbjct: 287 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 346

Query: 181 FAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240
           FAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL
Sbjct: 347 FAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 406

Query: 241 IGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300
           IGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL
Sbjct: 407 IGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 466

Query: 301 WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGA 349
           WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGA
Sbjct: 467 WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGA 520

[Close]

BLAST of Csor.00g196100 vs. NCBI nr
Match: XP_038905301.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 585 bits (1509), Expect = 3.57e-208
Identity = 284/366 (77.60%), Postives = 310/366 (84.70%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           ME+EI   LKVWISIW+CL YCYGVG++I  G TRFL IFPVI +FLYLPL L SLHLGG
Sbjct: 1   MEEEIQNFLKVWISIWICLSYCYGVGERIPHGITRFLFIFPVISLFLYLPLILRSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
           LTAFF+AWLANFKLLLFAAGQGPLA  GMSI     VASFP+K+KQ P QK+SE A NGG
Sbjct: 61  LTAFFIAWLANFKLLLFAAGQGPLAAPGMSIGTFCAVASFPVKLKQNPFQKTSEAAINGG 120

Query: 121 AATVIDPKSNQN-HRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDL 180
           +A + D  SN N HR+S  NY VKILLVAALVKAYDYTHILPQKL+W  LCFHIYF+L+L
Sbjct: 121 SARINDTNSNHNRHRQSLLNYTVKILLVAALVKAYDYTHILPQKLLWLFLCFHIYFLLEL 180

Query: 181 SFAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATK 240
           S AAA++AV+  GT+ELLPLFH PYLSTSLQDFWGRRWNL VTGILRP  +RPA S ATK
Sbjct: 181 SLAAAASAVAFGGTVELLPLFHQPYLSTSLQDFWGRRWNLVVTGILRPAVFRPAASAATK 240

Query: 241 LIGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKR 300
           L+G RWAPLAGV  TFLVSA MHELMFFYMSRQWPPPTWEV AFFV HGGCL  EM  KR
Sbjct: 241 LLGSRWAPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVMAFFVFHGGCLVAEMAVKR 300

Query: 301 LWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKNN 360
           LWID W+M PLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVV+EY IFGAF+KNN
Sbjct: 301 LWIDTWQMRPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVEEYAIFGAFVKNN 360

[Close]

BLAST of Csor.00g196100 vs. ExPASy TrEMBL
Match: A0A6J1K824 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493084 PE=3 SV=1)

HSP 1 Score: 696 bits (1797), Expect = 1.43e-252
Identity = 341/358 (95.25%), Postives = 343/358 (95.81%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           MEDEIG LLKVWISIW+CLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPL LHSLHLGG
Sbjct: 1   MEDEIGNLLKVWISIWICLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLKLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
           LTAFFVAWLANFKLLLFAAGQGPLATTGMSI     VASFPIKIKQI  QKSSEPA NGG
Sbjct: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQILPQKSSEPAGNGG 120

Query: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180
           AATVIDPKSNQNH ESPFNYAVKILLVAALVKAYDYTHIL QKLVWFLLCFHIYFILDLS
Sbjct: 121 AATVIDPKSNQNHCESPFNYAVKILLVAALVKAYDYTHILHQKLVWFLLCFHIYFILDLS 180

Query: 181 FAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240
           FAAASAAVSH GT+ELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL
Sbjct: 181 FAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240

Query: 241 IGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300
           IGPRWAPLAGVA TFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL
Sbjct: 241 IGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300

Query: 301 WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 353
           WIDAWKMPPLPA VSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN
Sbjct: 301 WIDAWKMPPLPAVVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 358

[Close]

BLAST of Csor.00g196100 vs. ExPASy TrEMBL
Match: A0A0A0LD00 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684680 PE=3 SV=1)

HSP 1 Score: 549 bits (1414), Expect = 4.16e-194
Identity = 265/369 (71.82%), Postives = 302/369 (81.84%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           MEDEIG  LKVWISIW+CL YCYGVG++I  G TRFL IFPVI +FLYLPL LHSLHLGG
Sbjct: 1   MEDEIGNFLKVWISIWICLSYCYGVGRRIPHGITRFLFIFPVISLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
           LTAFF+AWLANFKLLLF+AG GPLA  G+SI     +ASFP+K+KQ P QK+SE A +GG
Sbjct: 61  LTAFFIAWLANFKLLLFSAGLGPLAAPGISIGTFCAIASFPVKLKQTPLQKTSEFAIHGG 120

Query: 121 AATVIDPKSNQN-HRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDL 180
               +  KS+ N HR+SP +YAVKI LVA +VKAY+YTHILPQKL W  LCFHIYF+L+L
Sbjct: 121 ----LPSKSDHNRHRQSPLSYAVKIFLVAVMVKAYNYTHILPQKLHWLFLCFHIYFLLEL 180

Query: 181 SFAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATK 240
           S AAA++ V+  GT+ELLPLFH PY STSLQDFWGRRWNL VTGILRP  +RPA S A+K
Sbjct: 181 SLAAAASIVACGGTLELLPLFHQPYFSTSLQDFWGRRWNLVVTGILRPAVFRPAASAASK 240

Query: 241 LIGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKR 300
           L+G +  PLAGV  TFLVSA MHELMFFYMSRQWPPPTWEV+AFFV HGGCL  EM  KR
Sbjct: 241 LLGKKCGPLAGVTATFLVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVAEMAVKR 300

Query: 301 LWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKNN 360
           +WI  W+M PLP  VSVPVTVGFFFW+CFWLFFPPFIN+CKADVRVV+EY +FGAFLKNN
Sbjct: 301 VWIQRWRMRPLPWIVSVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFLKNN 360

Query: 361 IIPTTFFPS 363
           I P   +P+
Sbjct: 361 I-PINLWPN 364

[Close]

BLAST of Csor.00g196100 vs. ExPASy TrEMBL
Match: A0A1S3CFN5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC103500392 PE=3 SV=1)

HSP 1 Score: 541 bits (1394), Expect = 2.77e-190
Identity = 258/369 (69.92%), Postives = 300/369 (81.30%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           ME+EIG  LKVWIS W+CL YCYGVGK+I  G TRFL I PV+ +FLYLPL LHSLHLGG
Sbjct: 49  MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 108

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
           LTAFF+AWLANFKLLLF+AG GPL+  G+S+     +ASFP+K+KQ P QK+SE A NG 
Sbjct: 109 LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAINGE 168

Query: 121 AATVIDPKSNQN-HRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDL 180
               +  KS+ N H +SP +YAVKI LVAA+VKAY+YTHILPQKL W  LCFHIYF+L+L
Sbjct: 169 ----LPSKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLEL 228

Query: 181 SFAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATK 240
           S AAA++ V+  GT+ELLPLFH PY STSLQDFWGRRWNL VTGILRP  + PA S A+K
Sbjct: 229 SLAAAASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASK 288

Query: 241 LIGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKR 300
           L G RW P+ GV  TF+VSA MHELMFFYMSRQWPPPTWEV+AFFV HGGCL  EMV KR
Sbjct: 289 LFGSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKR 348

Query: 301 LWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKNN 360
           +WI  W M PLP+ V+VPVTVGFFFW+CFWLFFPPFIN+CKADVRVV+EY +FGAF+KNN
Sbjct: 349 VWIQKWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGAFVKNN 408

Query: 361 IIPTTFFPS 363
           I P++ FP+
Sbjct: 409 I-PSSLFPN 412

[Close]

BLAST of Csor.00g196100 vs. ExPASy TrEMBL
Match: A0A5A7U2E1 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G001430 PE=3 SV=1)

HSP 1 Score: 527 bits (1358), Expect = 1.29e-181
Identity = 250/356 (70.22%), Postives = 288/356 (80.90%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           ME+EIG  LKVWIS W+CL YCYGVGK+I  G TRFL I PV+ +FLYLPL LHSLHLGG
Sbjct: 1   MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
           LTAFF+AWLANFKLLLF+AG GPL+  G+S+     +ASFP+K+KQ P QK+SE A NG 
Sbjct: 61  LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAINGE 120

Query: 121 AATVIDPKSNQN-HRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDL 180
               +  KS+ N H +SP +YAVKI LVAA+VKAY+YTHILPQKL W  LCFHIYF+L+L
Sbjct: 121 ----LPSKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLEL 180

Query: 181 SFAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATK 240
           S AAA++ V+  GT+ELLPLFH PY STSLQDFWGRRWNL VTGILRP  + PA S A+K
Sbjct: 181 SLAAAASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASK 240

Query: 241 LIGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKR 300
           L G RW P+ GV  TF+VSA MHELMFFYMSRQWPPPTWEV+AFFV HGGCL  EMV KR
Sbjct: 241 LFGSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKR 300

Query: 301 LWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAF 350
           +WI  W M PLP+ V+VPVTVGFFFW+CFWLFFPPFIN+CKADVRVV+EY +FG F
Sbjct: 301 VWIQKWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGLF 352

[Close]

BLAST of Csor.00g196100 vs. ExPASy TrEMBL
Match: A0A5N5FGI4 (Acyl-CoA--sterol O-acyltransferase 1 OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_001856 PE=3 SV=1)

HSP 1 Score: 355 bits (911), Expect = 7.50e-118
Identity = 184/359 (51.25%), Postives = 238/359 (66.30%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           ME EIG  +KVW+S+++ L YCY + K + +G  R LLI P++ +FLYLPL+L SLHLGG
Sbjct: 1   MEGEIGNFIKVWLSVYLSLCYCYAIAKFVPKGVPRLLLIIPIVSLFLYLPLHLSSLHLGG 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATT-GMSI-----VASFPIKIKQIPSQKSSEPANNG 120
            TAFF++WLANFKLLLFA G+GPL +   +S+     VA  PIKI+Q P  K+    N  
Sbjct: 61  TTAFFISWLANFKLLLFAFGKGPLCSDPSISLLRFVAVACLPIKIQQNPYSKTQNKENPA 120

Query: 121 GAATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDL 180
                  PK  +N  +SP NYAVK+LL A LVK YDY   +  KL+W + CFHIYF L++
Sbjct: 121 -------PKITKNGTKSPLNYAVKVLLFALLVKLYDYKEHIHPKLLWIMYCFHIYFALEI 180

Query: 181 SFAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATK 240
               A+        +EL P F+ PYLS+SLQDFWGRRWN+ VT ILRP  Y P ++ AT+
Sbjct: 181 ILVIAATLARALLGLELEPQFNEPYLSSSLQDFWGRRWNIMVTSILRPTVYEPILALATR 240

Query: 241 LIGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKR 300
           ++G RWA LAGV  TF+VSA MHEL+F+Y+ R    PTWE+  FF+LHG CL  E+  K+
Sbjct: 241 VVGRRWASLAGVMGTFVVSAIMHELIFYYLGRT--QPTWEITWFFLLHGACLMAEIGVKK 300

Query: 301 LWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 353
             +   K   LP +VS P+TV F   T FWLFFPP +  CKADVR ++EY   GAF+K+
Sbjct: 301 A-VGKRKKLQLPRSVSGPLTVAFVTATVFWLFFPPLLR-CKADVRALEEYAAVGAFVKH 348

[Close]

BLAST of Csor.00g196100 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 255.4 bits (651), Expect = 7.0e-68
Identity = 142/353 (40.23%), Postives = 202/353 (57.22%), Query Frame = 0


Query: 5   IGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGGLTAF 64
           +   +K W  + + L Y + + K + +G  R +L FPV  IF  +P  ++SLHL G+TAF
Sbjct: 1   MASFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAF 60

Query: 65  FVAWLANFKLLLFAAGQGPLATTGMSI-------VASFPIKIKQIPSQKSSEPANNGGAA 124
           F+AWLANFKLLLFA G+GPL++    +       V+  PIKI+  P    +         
Sbjct: 61  FIAWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTH-------- 120

Query: 125 TVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLSFA 184
                 S++   E P  Y +K + V  ++KAY+Y+  LP+K+V  L   HIYF L++  A
Sbjct: 121 ------SHEGSTEGPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILA 180

Query: 185 AASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKLIG 244
           A +AAV     +EL P F+ PYL+TSLQDFWGRRWNL VTGILRP  Y P++   + ++G
Sbjct: 181 ATAAAVRAMSDLELEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLG 240

Query: 245 PRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRLWI 304
           P ++ +     TF+VS  MHEL+FFYM R    P W++  FF+++G C  VE+  K+   
Sbjct: 241 PNYSQILAAFGTFVVSGIMHELIFFYMGRL--RPDWKMMWFFLINGFCTTVEIAIKKTIN 300

Query: 305 DAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAF 351
             W+    P A+S  +T+ F   T  WLF P F N C    + + EY   GAF
Sbjct: 301 GRWR---FPKAISQVLTLTFVMVTALWLFLPEF-NRCNIVEKALDEYAAIGAF 332

[Close]

BLAST of Csor.00g196100 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.1e-57
Identity = 136/334 (40.72%), Postives = 179/334 (53.59%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           ME++   L++VWIS  + L YCY +  K+ +G  R L I PV  +FL LPL L  +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPL-----ATTGMSIVASFPIKIKQIPSQKSSEPANNGG 120
           ++  F++WLANFKLLLFA  +GPL       +     A  PIKI+Q PS           
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPPKLSRFICFACLPIKIRQDPS----------- 120

Query: 121 AATVIDPKSNQNHRESP---FNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFIL 180
                 P +  N    P   +  AVKIL++  L+  Y+Y   LP+ +V  L C HIY  +
Sbjct: 121 ------PNAIPNLHPKPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEV 180

Query: 181 DLSFAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTA 240
           +L      A VS      + P+F+ PYL+TSLQDFW RRWNL V+ +LR   + P     
Sbjct: 181 ELVLVFVGAVVSTLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFF 240

Query: 241 TKLIGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVA 300
            +++ P  A  AGV  +F VS  MHEL++FYM R+  PPTWEV  FFVLHG   A E+  
Sbjct: 241 KRILSPDGAMFAGVMASFFVSGLMHELLYFYMIRK--PPTWEVTCFFVLHGAATATEIAV 300

Query: 301 KRLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLF 327
           KR     W  PP   AVS  V + F   T  WLF
Sbjct: 301 KR---TQWLRPP-HRAVSGLVVLTFVSVTGVWLF 311

[Close]

BLAST of Csor.00g196100 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.1e-57
Identity = 137/359 (38.16%), Postives = 189/359 (52.65%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           ME+E+  L+KVW+   + + YCY    +I  G  R L + PV  +FL LPL + S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
            TAFF++WLANFKL+LF+  QGPL     ++        FPIK++Q P  ++  P     
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPSNLSRFVCFTCFPIKLQQNPKPQNQMP----- 120

Query: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180
                            + +AVK+     L+  Y+Y   +   ++  L   HIY   ++ 
Sbjct: 121 ----------------KWGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEIL 180

Query: 181 FAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240
            A     +S +   +L P F+ PYLSTSLQDFWGRRWNL V  ILRP  Y P    A + 
Sbjct: 181 LAPLKVLLSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRK 240

Query: 241 IGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300
           +    A   GV  +FLVS  +HEL+FFY +R+   PT EV  FFVLHG C A E  AKR 
Sbjct: 241 MNSDQALFLGVFASFLVSGVVHELIFFYFTRE--SPTGEVTLFFVLHGVCTAAECAAKRT 300

Query: 301 -WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFLKN 354
             +  WK+  +   VS  +TVGF   T  WLFFP    +   + R+  E  +F  F+K+
Sbjct: 301 RLVRRWKVSQM---VSRLLTVGFVVMTGGWLFFPHLARSGMIE-RLADEAFLFIGFVKH 332

[Close]

BLAST of Csor.00g196100 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 217.2 bits (552), Expect = 2.1e-56
Identity = 132/335 (39.40%), Postives = 182/335 (54.33%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           M++E+  L+KVW+S  + + YCY +  +I  G  RFL + PV+ +FL LPL   SLHL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSI-----VASFPIKIKQIPSQKSSEPANNGG 120
           +TAFF+ WLANFKL+LF+  +GPL     +         FPIK++Q              
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIPTNFPRFFCFTCFPIKVQQ-------------- 120

Query: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180
                +PKS QNH      +A+K+ + A L+  Y Y   L   ++  L   H+Y  +++ 
Sbjct: 121 -----NPKS-QNHLPK-LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEII 180

Query: 181 FAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240
                  V  +   +L P  + PYL+TSLQDFWGRRWNL V  ILRP  Y P    + + 
Sbjct: 181 LTFVKVVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERK 240

Query: 241 IGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300
           +   WA   G+   F+VS  +HEL+FFY+ R+   PT EV  FFVLHG C AVE+  K+ 
Sbjct: 241 MSSGWALFPGILAAFIVSGLVHELLFFYLIREM--PTGEVTLFFVLHGVCTAVELAVKKK 300

Query: 301 WIDA--WKMPPLPAAVSVPVTVGFFFWTCFWLFFP 329
              A  W++ P    VS  +TVGF F T  WLF P
Sbjct: 301 TTVAQRWRLSP---GVSRVLTVGFVFVTGGWLFTP 309

[Close]

BLAST of Csor.00g196100 vs. TAIR 10
Match: AT1G34500.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 211.8 bits (538), Expect = 8.9e-55
Identity = 123/339 (36.28%), Postives = 184/339 (54.28%), Query Frame = 0


Query: 1   MEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLHSLHLGG 60
           ME+E+   + VWIS  + + YCY +   I  G  R   + P+  +F  LPL   S+H   
Sbjct: 1   MEEELKNFIIVWISAIISVSYCYYISANIKTGVLRLFSVLPICGLFFVLPLFFSSVHFSS 60

Query: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIV-----ASFPIKIKQIPSQKSSEPANNGG 120
            TAF+++ +A+ KL+LFA  QGPL     +++       FPIK+++ P  +         
Sbjct: 61  STAFYLSEMASLKLILFAFDQGPLFPVAPNLIQFVCFTCFPIKLQRNPKSQ--------- 120

Query: 121 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 180
                 P  N  H+ +   +A+KI++   ++  Y+Y+H LPQ ++  L   H+Y  L++ 
Sbjct: 121 ------PSQNHFHKRA---FAIKIMIFGVVLHVYNYSHFLPQTVLLSLCFLHLYVELEIL 180

Query: 181 FAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240
                  +S A   +L P F+ PYL+TSLQDFWGRRWNL V+ +LR   Y P      + 
Sbjct: 181 LGPLKVLLSMALGCDLEPQFNKPYLATSLQDFWGRRWNLMVSSVLRSGIYNPVRCACQRP 240

Query: 241 IGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKR- 300
           +   WA   G  VTFLVS   HEL++FY++R+   PTWEV  FFVL+G C   E+  KR 
Sbjct: 241 MNSGWARFMGYLVTFLVSGLFHELVYFYITRE--TPTWEVTLFFVLNGVCTGTEVAVKRT 300

Query: 301 LWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINT 334
            ++  W   P+ ++VS  +T+GF   T   LFFP FI +
Sbjct: 301 AFLQRWW--PVRSSVSRLLTMGFVVVTGGLLFFPLFIRS 317

The following BLAST results are available for this feature:

BLAST of Csor.00g196100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	9.9e-67	40.23	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9FJ72	1.6e-56	40.72	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]
Q9FJ75	1.6e-56	38.16	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9FJ76	3.0e-55	39.40	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9XGY6	8.7e-55	36.91	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]

BLAST of Csor.00g196100 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6606896.1	3.90e-274	100.00	Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sor...	[more]
XP_023523549.1	3.99e-266	96.48	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022998457.1	2.96e-252	95.25	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
KAG7036600.1	1.67e-251	98.59	Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. arg...	[more]
XP_038905301.1	3.57e-208	77.60	acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida]	[more]

BLAST of Csor.00g196100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K824	1.43e-252	95.25	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A0A0LD00	4.16e-194	71.82	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684680 PE=...	[more]
A0A1S3CFN5	2.77e-190	69.92	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC10350039...	[more]
A0A5A7U2E1	1.29e-181	70.22	Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A5N5FGI4	7.50e-118	51.25	Acyl-CoA--sterol O-acyltransferase 1 OS=Pyrus ussuriensis x Pyrus communis OX=24...	[more]

BLAST of Csor.00g196100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	7.0e-68	40.23	acyl-CoA sterol acyl transferase 1	[more]
AT5G55380.1	1.1e-57	40.72	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	1.1e-57	38.16	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55340.1	2.1e-56	39.40	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT1G34500.1	8.9e-55	36.28	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 193..280 e-value: 7.4E-14 score: 51.8
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 2..354
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 2..354

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g196100.m01	Csor.00g196100.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity