Carg04144 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04144
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAlpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
LocationCarg_Chr19: 7900231 .. 7901535 (+)
RNA-Seq ExpressionCarg04144
SyntenyCarg04144
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTATTAATCAGAAACCTCGCCTCAAAGATTCTGCTCTGAGACGTTTTAAATTGGTTATTCTTGTGACATTATTGGGGTTCTTGCTGTTTATGATCCTTATGGAAACGAATTTGTTTCAGAATTTTGCTCCTTGGTCTTCTGATAATATTCGTGAGGATCCATATGCTGGCTTTGGTCGTTTTGATTCTAATGATGAACTTAAGCTGCCCAAACAAACTGAGTTATCGATTCGTTTAGAAAAGCGAAATCGATTGCCACTTCGAAACTTAGATCTGTATCCAAATCTAGCCAAGGATCATATACCTATTTTACTTTATGTGCACAATCGACCGCAGTATCTTCGAGTCGTAATCGATAGCTTGTCCAAAGTTACAGGAATAAGTGAAACCTTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGCTTGTCCAGAACATCAGGTTTTGCTCAGTGAAACAGATTTTTGCTCCATACTCTCCTCATCTCTTTCCCGACAGCTTTCCCGGTGTCTCGCCTACAGATTGTATCGGGAAACATTATCCTATGCTAACAACAAATTGCAGAGGGAATCCTGATCAGTATGGAAACCATCGGTTGCCTAAAATCGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAACACGGTGTGGGATGGATTGGAGGAGACGCGGGGGTTGTCAGGTCATATTTTGTTTATTGAGGAAGATCATTTTATATTGCCAAATGCGTATCGGAATCTGCAGTTACTCGTGTCGTTGAAGCCTAAAAAATGTCCTGAATGCTATTCTGTGAATCTAGCTCCTAGTGATGTGAAATCAAGAGGTGAGGGTAACAATTTTCTCGTTGCAGAGAGGATGGGAAATATTGGTTATGCTTTCAATAGGTCTGTTTGGAAGAAAATCCATAAGAAAGCAAAGGAGTTCTGTTTTTTTGATGAATACAATTGGGATATAACAATGTGGGCAACCGTTTTTCCCTCGTTTGGCAGCCCTGTTTATTCACTTCGAGGCCCTTGGACGAGCGCAATTCACTTTGGCAAATGTGGTTTACACCAGGGTCAGGACGAGGGGGATGTTTGTATCGATAATGGTTTGTTAAACATCGACGTTAAAGATATCGACAAAGTTGTGAACATTAATTCGGAGTGGCCAGTCGAAGTCTTCAGAGGTCAGCCTGGCTATGGAGCTGGGTTCAGGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCAGTTGTGTCTAAATTTTGCTCAAATGTATCGTTCCACGCCGAACGTATCCCGTTCATGA

mRNA sequence

ATGGCTATTAATCAGAAACCTCGCCTCAAAGATTCTGCTCTGAGACGTTTTAAATTGGTTATTCTTGTGACATTATTGGGGTTCTTGCTGTTTATGATCCTTATGGAAACGAATTTGTTTCAGAATTTTGCTCCTTGGTCTTCTGATAATATTCGTGAGGATCCATATGCTGGCTTTGGTCGTTTTGATTCTAATGATGAACTTAAGCTGCCCAAACAAACTGAGTTATCGATTCGTTTAGAAAAGCGAAATCGATTGCCACTTCGAAACTTAGATCTGTATCCAAATCTAGCCAAGGATCATATACCTATTTTACTTTATGTGCACAATCGACCGCAGTATCTTCGAGTCGTAATCGATAGCTTGTCCAAAGTTACAGGAATAAGTGAAACCTTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGCTTGTCCAGAACATCAGGTTTTGCTCAGTGAAACAGATTTTTGCTCCATACTCTCCTCATCTCTTTCCCGACAGCTTTCCCGGTGTCTCGCCTACAGATTGTATCGGGAAACATTATCCTATGCTAACAACAAATTGCAGAGGGAATCCTGATCAGTATGGAAACCATCGGTTGCCTAAAATCGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAACACGGTGTGGGATGGATTGGAGGAGACGCGGGGGTTGTCAGGTCATATTTTGTTTATTGAGGAAGATCATTTTATATTGCCAAATGCGTATCGGAATCTGCAGTTACTCGTGTCGTTGAAGCCTAAAAAATGTCCTGAATGCTATTCTGTGAATCTAGCTCCTAGTGATGTGAAATCAAGAGGTGAGGGTAACAATTTTCTCGTTGCAGAGAGGATGGGAAATATTGGTTATGCTTTCAATAGGTCTGTTTGGAAGAAAATCCATAAGAAAGCAAAGGAGTTCTGTTTTTTTGATGAATACAATTGGGATATAACAATGTGGGCAACCGTTTTTCCCTCGTTTGGCAGCCCTGTTTATTCACTTCGAGGCCCTTGGACGAGCGCAATTCACTTTGGCAAATGTGGTTTACACCAGGGTCAGGACGAGGGGGATGTTTGTATCGATAATGGTTTGTTAAACATCGACGTTAAAGATATCGACAAAGTTGTGAACATTAATTCGGAGTGGCCAGTCGAAGTCTTCAGAGGTCAGCCTGGCTATGGAGCTGGGTTCAGGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCAGTTGTGTCTAAATTTTGCTCAAATGTATCGTTCCACGCCGAACGTATCCCGTTCATGA

Coding sequence (CDS)

ATGGCTATTAATCAGAAACCTCGCCTCAAAGATTCTGCTCTGAGACGTTTTAAATTGGTTATTCTTGTGACATTATTGGGGTTCTTGCTGTTTATGATCCTTATGGAAACGAATTTGTTTCAGAATTTTGCTCCTTGGTCTTCTGATAATATTCGTGAGGATCCATATGCTGGCTTTGGTCGTTTTGATTCTAATGATGAACTTAAGCTGCCCAAACAAACTGAGTTATCGATTCGTTTAGAAAAGCGAAATCGATTGCCACTTCGAAACTTAGATCTGTATCCAAATCTAGCCAAGGATCATATACCTATTTTACTTTATGTGCACAATCGACCGCAGTATCTTCGAGTCGTAATCGATAGCTTGTCCAAAGTTACAGGAATAAGTGAAACCTTGCTAATTGTTAGCCATGATGGGTACTTTGAAGAAATGGACAAGCTTGTCCAGAACATCAGGTTTTGCTCAGTGAAACAGATTTTTGCTCCATACTCTCCTCATCTCTTTCCCGACAGCTTTCCCGGTGTCTCGCCTACAGATTGTATCGGGAAACATTATCCTATGCTAACAACAAATTGCAGAGGGAATCCTGATCAGTATGGAAACCATCGGTTGCCTAAAATCGTTTCGTTAAAGCACCATTGGTGGTGGATGATGAACACGGTGTGGGATGGATTGGAGGAGACGCGGGGGTTGTCAGGTCATATTTTGTTTATTGAGGAAGATCATTTTATATTGCCAAATGCGTATCGGAATCTGCAGTTACTCGTGTCGTTGAAGCCTAAAAAATGTCCTGAATGCTATTCTGTGAATCTAGCTCCTAGTGATGTGAAATCAAGAGGTGAGGGTAACAATTTTCTCGTTGCAGAGAGGATGGGAAATATTGGTTATGCTTTCAATAGGTCTGTTTGGAAGAAAATCCATAAGAAAGCAAAGGAGTTCTGTTTTTTTGATGAATACAATTGGGATATAACAATGTGGGCAACCGTTTTTCCCTCGTTTGGCAGCCCTGTTTATTCACTTCGAGGCCCTTGGACGAGCGCAATTCACTTTGGCAAATGTGGTTTACACCAGGGTCAGGACGAGGGGGATGTTTGTATCGATAATGGTTTGTTAAACATCGACGTTAAAGATATCGACAAAGTTGTGAACATTAATTCGGAGTGGCCAGTCGAAGTCTTCAGAGGTCAGCCTGGCTATGGAGCTGGGTTCAGGGGTTGGGGAGGTTGGGGTGATGAAAGAGACCGTCAGTTGTGTCTAAATTTTGCTCAAATGTATCGTTCCACGCCGAACGTATCCCGTTCATGA

Protein sequence

MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFGRFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVIDSLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDCIGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNRSVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQDEGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLNFAQMYRSTPNVSRS
Homology
BLAST of Carg04144 vs. NCBI nr
Match: KAG7011860.1 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 917.5 bits (2370), Expect = 4.2e-263
Identity = 434/434 (100.00%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120
           RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
           IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSTPNVSRS 435
           FAQMYRSTPNVSRS
Sbjct: 421 FAQMYRSTPNVSRS 434

BLAST of Carg04144 vs. NCBI nr
Match: KAG6572221.1 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 916.0 bits (2366), Expect = 1.2e-262
Identity = 433/434 (99.77%), Postives = 433/434 (99.77%), Query Frame = 0

Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120
           RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
           IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYG GFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGVGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSTPNVSRS 435
           FAQMYRSTPNVSRS
Sbjct: 421 FAQMYRSTPNVSRS 434

BLAST of Carg04144 vs. NCBI nr
Match: XP_023554482.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 912.9 bits (2358), Expect = 1.0e-261
Identity = 429/434 (98.85%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60
           MA+NQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG
Sbjct: 1   MAMNQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120
           RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPN+AKDHIPI+LYVHNRPQYLRVVID
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNIAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
           +GKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 MGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSTPNVSRS 435
           FAQMYRSTPN+SRS
Sbjct: 421 FAQMYRSTPNISRS 434

BLAST of Carg04144 vs. NCBI nr
Match: XP_022952309.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbita moschata])

HSP 1 Score: 910.6 bits (2352), Expect = 5.2e-261
Identity = 430/434 (99.08%), Postives = 432/434 (99.54%), Query Frame = 0

Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120
           RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPI+LYVHNRPQYLRVVID
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
           +GKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 MGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQ LVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQFLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGS VYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSSVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSTPNVSRS 435
           FAQMYRSTPNVSRS
Sbjct: 421 FAQMYRSTPNVSRS 434

BLAST of Carg04144 vs. NCBI nr
Match: XP_022969101.1 (alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbita maxima])

HSP 1 Score: 894.4 bits (2310), Expect = 3.8e-256
Identity = 420/433 (97.00%), Postives = 428/433 (98.85%), Query Frame = 0

Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNF PW SDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60

Query: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120
           RFDSND LKLPKQTELS+RLEKRNRLPLRNLDLYPNLAKDHIPI+LYVHNRPQYLRVVID
Sbjct: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFP+SFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180

Query: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
            GKHYPM+TTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLV+LKPKKCPEC++VNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDID+VVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSTPNVSR 434
           FAQMYRS PNVSR
Sbjct: 421 FAQMYRSMPNVSR 433

BLAST of Carg04144 vs. ExPASy Swiss-Prot
Match: Q9FT88 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Arabidopsis thaliana OX=3702 GN=GNT2 PE=1 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 1.8e-160
Identity = 270/423 (63.83%), Postives = 327/423 (77.30%), Query Frame = 0

Query: 5   QKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFGRFDS 64
           +K RL+D+ L R  ++  VTL   L+ + +  T L  +      D + +D  A      +
Sbjct: 6   KKQRLRDTGLCRLGILFAVTLSIVLMLVSVPRTALNGSSIDDDLDGLDKDLEAKL----N 65

Query: 65  NDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVIDSLSK 124
              L + +   +S+RL +RN    RN DL+P+LAKD + I+LYVHNR QY RV ++SLSK
Sbjct: 66  ASLLSVARGNRMSLRLHRRNHFSPRNTDLFPDLAKDRVVIVLYVHNRAQYFRVTVESLSK 125

Query: 125 VTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDCIGKH 184
           V GISETLLIVSHDGYFEEM+++V++I+FC VKQIF+PYSPH++  SFPGV+  DC  K 
Sbjct: 126 VKGISETLLIVSHDGYFEEMNRIVESIKFCQVKQIFSPYSPHIYRTSFPGVTLNDCKNKG 185

Query: 185 YPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFI 244
                 +C GNPDQYGNHR PKIVSLKHHWWWMMNTVWDGLEET+G  GHILFIEEDHF+
Sbjct: 186 -DEAKGHCEGNPDQYGNHRSPKIVSLKHHWWWMMNTVWDGLEETKGHEGHILFIEEDHFL 245

Query: 245 LPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNRSVWK 304
            PNAYRN+Q L  LKP KCP+C++ NLAPSDVKSRGEG   LVAERMGN+GY+FNRSVW+
Sbjct: 246 FPNAYRNIQTLTRLKPAKCPDCFAANLAPSDVKSRGEGLESLVAERMGNVGYSFNRSVWE 305

Query: 305 KIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQ-DEGD 364
            IH+KA+EFCFFD+YNWDITMWATVFPSFGSPVY+LRGP TSA+HFGKCGLHQG+ DEGD
Sbjct: 306 NIHQKAREFCFFDDYNWDITMWATVFPSFGSPVYTLRGPRTSAVHFGKCGLHQGRGDEGD 365

Query: 365 VCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLNFAQ 424
            CIDNG++NI+VK+ DKVVNI   W V V++ Q GY AGF GWGGWGD+RDR LCL+FA 
Sbjct: 366 -CIDNGVVNIEVKETDKVVNIKEGWGVRVYKHQAGYKAGFEGWGGWGDDRDRHLCLDFAT 422

Query: 425 MYR 427
           MYR
Sbjct: 426 MYR 422

BLAST of Carg04144 vs. ExPASy Swiss-Prot
Match: Q10469 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Homo sapiens OX=9606 GN=MGAT2 PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.1e-48
Identity = 118/357 (33.05%), Postives = 189/357 (52.94%), Query Frame = 0

Query: 88  LRNLDLYPNLAKDHIPILLYVHNRPQYLRVVIDSLSKVTGISETLLIVSHDGYFEEMDKL 147
           LRN+D     A   + +++ VHNRP+YLR+++DSL K  GI   L+I SHD +  E+++L
Sbjct: 104 LRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVLVIFSHDFWSTEINQL 163

Query: 148 VQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC---IGKHYPMLTTNC--RGNPDQYGNH 207
           +  + FC V Q+F P+S  L+P+ FPG  P DC   + K+   L   C     PD +G++
Sbjct: 164 IAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKN-AALKLGCINAEYPDSFGHY 223

Query: 208 RLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFILPNAYRNLQLLVSLKPKK 267
           R  K    KHHWWW ++ VW+ ++  R  +G ILF+EEDH++ P+ Y   + +  LK ++
Sbjct: 224 REAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQE 283

Query: 268 CPEC-------YSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNRSVWKKIHKKAKEFCF 327
           CPEC       YS + +   +  + +   +   E   N+G A  R+ ++K+ +    FC 
Sbjct: 284 CPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEH--NMGLALTRNAYQKLIECTDTFCT 343

Query: 328 FDEYNWDITM-WATV--FPSFGSPVYSLRGPWTSAI-HFGKCGLHQGQ----DEGDVCID 387
           +D+YNWD T+ + TV   P F    + +  P    I H G CG+H  +          I+
Sbjct: 344 YDDYNWDWTLQYLTVSCLPKF----WKVLVPQIPRIFHAGDCGMHHKKTCRPSTQSAQIE 403

Query: 388 NGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLNFAQM 425
           + L N       + + I+ ++ V V    P      R  GGWGD RD +LC ++ ++
Sbjct: 404 SLLNNNKQYMFPETLTISEKFTV-VAISPP------RKNGGWGDIRDHELCKSYRRL 446

BLAST of Carg04144 vs. ExPASy Swiss-Prot
Match: Q09326 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Rattus norvegicus OX=10116 GN=Mgat2 PE=1 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.2e-48
Identity = 114/356 (32.02%), Postives = 182/356 (51.12%), Query Frame = 0

Query: 88  LRNLDLYPNLAKDHIPILLYVHNRPQYLRVVIDSLSKVTGISETLLIVSHDGYFEEMDKL 147
           LRN+D     +   + +++ VHNRP+YLR++IDSL K  GI E L+I SHD +  E++ L
Sbjct: 99  LRNVDKDGTWSPGELVLVVQVHNRPEYLRLLIDSLRKAQGIREVLVIFSHDFWSAEINSL 158

Query: 148 VQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDCIG--KHYPMLTTNC--RGNPDQYGNHR 207
           + ++ FC V Q+F P+S  L+P  FPG  P DC    K    L   C     PD +G++R
Sbjct: 159 ISSVDFCPVLQVFFPFSIQLYPSEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYR 218

Query: 208 LPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFILPNAYRNLQLLVSLKPKKC 267
             K    KHHWWW ++ VW+ ++  +  +G ILF+EEDH++ P+ Y   + +  LK ++C
Sbjct: 219 EAKFSQTKHHWWWKLHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQEC 278

Query: 268 PECYSVNLAPSDVKSRGEGNNFLVAERMG---------NIGYAFNRSVWKKIHKKAKEFC 327
           P C  ++L          G    +A+++          N+G A  R  ++K+ +    FC
Sbjct: 279 PGCDVLSLGTYTTIRSFYG----IADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFC 338

Query: 328 FFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAI-HFGKCGLHQGQDEGDVCIDNGLLNI 387
            +D+YNWD T+           V+ +  P    I H G CG+H  +     C      + 
Sbjct: 339 TYDDYNWDWTLQYLTLACL-PKVWKVLVPQAPRIFHAGDCGMHHKK----TCRP----ST 398

Query: 388 DVKDIDKVVNINSEW--PVEVFRGQPGYGAGF---RGWGGWGDERDRQLCLNFAQM 425
               I+ ++N N ++  P  +  G+    A     R  GGWGD RD +LC ++ ++
Sbjct: 399 QSAQIESLLNNNKQYLFPETLVIGEKFPMAAISPPRKNGGWGDIRDHELCKSYRRL 441

BLAST of Carg04144 vs. ExPASy Swiss-Prot
Match: O19071 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Sus scrofa OX=9823 GN=MGAT2 PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 9.4e-48
Identity = 112/356 (31.46%), Postives = 185/356 (51.97%), Query Frame = 0

Query: 88  LRNLDLYPNLAKDHIPILLYVHNRPQYLRVVIDSLSKVTGISETLLIVSHDGYFEEMDKL 147
           LRN+D   +     + +++ VHNR +YL++++DSL K  GI   L+I SHD +  E+++L
Sbjct: 104 LRNVDKVSSWVPRELVLVVQVHNRAEYLKLLLDSLRKAQGIDNVLVIFSHDFWSTEINQL 163

Query: 148 VQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDCIG--KHYPMLTTNC--RGNPDQYGNHR 207
           +  + FC V Q+F P+S  L+P+ FPG  P DC    +    L   C     PD +G++R
Sbjct: 164 IAGVDFCPVLQVFFPFSIQLYPNEFPGTDPRDCPRDLEKNAALKMGCINAEYPDSFGHYR 223

Query: 208 LPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFILPNAYRNLQLLVSLKPKKC 267
             K    KHHWWW ++ VW+ ++  R  +G ILF+EEDH++ P+ Y   + + +LK ++C
Sbjct: 224 EAKFSQTKHHWWWKLHFVWERVKVLRDYAGLILFLEEDHYVAPDFYHVFKKMWNLKQQEC 283

Query: 268 PEC-------YSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNRSVWKKIHKKAKEFCFF 327
           PEC       Y+   +  DV  + +   +   E   N+G A  R  ++K+ +    FC +
Sbjct: 284 PECDVLSLGTYTTVRSFRDVADKVDVKTWKSTEH--NMGLALTRDAYQKLIECTDTFCTY 343

Query: 328 DEYNWDITM-WATV--FPSFGSPVYSLRGPWTSAI-HFGKCGLHQGQDEGDVCIDNGLLN 387
           D+YNWD T+ + TV   P F    + +  P    I H G CG+H  +     C      +
Sbjct: 344 DDYNWDWTLQYLTVSCLPKF----WKVLVPQVPRIFHAGDCGMHHKK----TCRP----S 403

Query: 388 IDVKDIDKVVNINSEW----PVEVFRGQPGYGAGFRGWGGWGDERDRQLCLNFAQM 425
                I+ ++N N ++     + +        +  R  GGWGD RD +LC ++ ++
Sbjct: 404 TQSAQIESLLNSNKQYMFPETLTISEKLTAALSPPRKNGGWGDIRDHELCKSYRRL 445

BLAST of Carg04144 vs. ExPASy Swiss-Prot
Match: Q921V5 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Mus musculus OX=10090 GN=Mgat2 PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.1e-47
Identity = 112/356 (31.46%), Postives = 181/356 (50.84%), Query Frame = 0

Query: 88  LRNLDLYPNLAKDHIPILLYVHNRPQYLRVVIDSLSKVTGISETLLIVSHDGYFEEMDKL 147
           LRN+      +   + +++ VHNRP+YLR++IDSL K  GI E L+I SHD +  E++ L
Sbjct: 99  LRNVGNDGTWSPGELVLVVQVHNRPEYLRLLIDSLRKAQGIQEVLVIFSHDFWSAEINSL 158

Query: 148 VQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDCIG--KHYPMLTTNC--RGNPDQYGNHR 207
           +  + FC V Q+F P+S  L+P+ FPG  P DC    K    L   C     PD +G++R
Sbjct: 159 ISRVDFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLKKNAALKLGCINAEYPDSFGHYR 218

Query: 208 LPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFILPNAYRNLQLLVSLKPKKC 267
             K    KHHWWW ++ VW+ ++  +  +G ILF+EEDH++ P+ Y   + +  LK ++C
Sbjct: 219 EAKFSQTKHHWWWKLHFVWERVKVLQDYTGLILFLEEDHYLAPDFYHVFKKMWKLKQQEC 278

Query: 268 PECYSVNLAPSDVKSRGEGNNFLVAERMG---------NIGYAFNRSVWKKIHKKAKEFC 327
           P C  ++L          G    +A+++          N+G A  R  ++K+ +    FC
Sbjct: 279 PGCDVLSLGTYTTIRSFYG----IADKVDVKTWKSTEHNMGLALTRDAYQKLIECTDTFC 338

Query: 328 FFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAI-HFGKCGLHQGQDEGDVCIDNGLLNI 387
            +D+YNWD T+           ++ +  P    I H G CG+H  +     C      + 
Sbjct: 339 TYDDYNWDWTLQYLTLACL-PKIWKVLVPQAPRIFHAGDCGMHHKK----TCRP----ST 398

Query: 388 DVKDIDKVVNINSEW--PVEVFRGQPGYGAGF---RGWGGWGDERDRQLCLNFAQM 425
               I+ ++N N ++  P  +  G+    A     R  GGWGD RD +LC ++ ++
Sbjct: 399 QSAQIESLLNSNKQYLFPETLVIGEKFPMAAISPPRKNGGWGDIRDHELCKSYRRL 441

BLAST of Carg04144 vs. ExPASy TrEMBL
Match: A0A6J1GLB7 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455025 PE=3 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 2.5e-261
Identity = 430/434 (99.08%), Postives = 432/434 (99.54%), Query Frame = 0

Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60

Query: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120
           RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPI+LYVHNRPQYLRVVID
Sbjct: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180

Query: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
           +GKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 MGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQ LVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQFLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGS VYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSSVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSTPNVSRS 435
           FAQMYRSTPNVSRS
Sbjct: 421 FAQMYRSTPNVSRS 434

BLAST of Carg04144 vs. ExPASy TrEMBL
Match: A0A6J1HWS5 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468194 PE=3 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 1.8e-256
Identity = 420/433 (97.00%), Postives = 428/433 (98.85%), Query Frame = 0

Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60
           MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNF PW SDNIREDPYAGFG
Sbjct: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFGPWFSDNIREDPYAGFG 60

Query: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120
           RFDSND LKLPKQTELS+RLEKRNRLPLRNLDLYPNLAKDHIPI+LYVHNRPQYLRVVID
Sbjct: 61  RFDSNDVLKLPKQTELSVRLEKRNRLPLRNLDLYPNLAKDHIPIVLYVHNRPQYLRVVID 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180
           SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFP+SFPGVSPTDC
Sbjct: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPNSFPGVSPTDC 180

Query: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
            GKHYPM+TTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE
Sbjct: 181 KGKHYPMITTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLV+LKPKKCPEC++VNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECHAVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD
Sbjct: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360

Query: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           EGDVCIDNGLLNIDVKDID+VVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 EGDVCIDNGLLNIDVKDIDEVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSTPNVSR 434
           FAQMYRS PNVSR
Sbjct: 421 FAQMYRSMPNVSR 433

BLAST of Carg04144 vs. ExPASy TrEMBL
Match: A0A5A7UTF0 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold295G00360 PE=3 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 3.8e-225
Identity = 369/430 (85.81%), Postives = 396/430 (92.09%), Query Frame = 0

Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60
           MAINQKPRLKD+AL+RFKLV+ VTLLG LLFMIL ETN   NFA WSSD+I EDPY GFG
Sbjct: 1   MAINQKPRLKDAALKRFKLVVFVTLLGLLLFMILTETNSSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120
            FDSN EL+LPKQ+ELSIRLEKRNRLP RNLDLYP LAK+HIPI+LYVHNRPQYLRVV+D
Sbjct: 61  HFDSNVELRLPKQSELSIRLEKRNRLPPRNLDLYPKLAKNHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180
           SL+KV GISETLL+VSHDGYFEEMDKLVQ+IRFC VKQIFAPYSPHLFP+SFPGVSP DC
Sbjct: 121 SLAKVIGISETLLVVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
            GK  P    +CRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGL+ET+G+SGHILFIEE
Sbjct: 181 KGKDDPK-AVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLV+LKPKKCPECYSVNLAPSDVKSRGEG+NFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           +VW+KIHK+AKEFCFFDEYNWDITMWATV+PSFG PVYSLRG W SA+HFGKCGLHQGQ 
Sbjct: 301 TVWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGTWASAVHFGKCGLHQGQG 360

Query: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           + DVCIDNG+LNIDVKDIDKV NINSEW V+VFR QPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 DRDVCIDNGVLNIDVKDIDKVANINSEWRVDVFRDQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSTPN 431
           FA MY S PN
Sbjct: 421 FAHMYHSVPN 429

BLAST of Carg04144 vs. ExPASy TrEMBL
Match: A0A1S3CG23 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis melo OX=3656 GN=LOC103500510 PE=3 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 3.8e-225
Identity = 369/430 (85.81%), Postives = 396/430 (92.09%), Query Frame = 0

Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60
           MAINQKPRLKD+AL+RFKLV+ VTLLG LLFMIL ETN   NFA WSSD+I EDPY GFG
Sbjct: 1   MAINQKPRLKDAALKRFKLVVFVTLLGLLLFMILTETNSSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120
            FDSN EL+LPKQ+ELSIRLEKRNRLP RNLDLYP LAK+HIPI+LYVHNRPQYLRVV+D
Sbjct: 61  HFDSNVELRLPKQSELSIRLEKRNRLPPRNLDLYPKLAKNHIPIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180
           SL+KV GISETLL+VSHDGYFEEMDKLVQ+IRFC VKQIFAPYSPHLFP+SFPGVSP DC
Sbjct: 121 SLAKVIGISETLLVVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
            GK  P    +CRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGL+ET+G+SGHILFIEE
Sbjct: 181 KGKDDPK-AVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLV+LKPKKCPECYSVNLAPSDVKSRGEG+NFLVAERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLVAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           +VW+KIHK+AKEFCFFDEYNWDITMWATV+PSFG PVYSLRG W SA+HFGKCGLHQGQ 
Sbjct: 301 TVWRKIHKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGTWASAVHFGKCGLHQGQG 360

Query: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           + DVCIDNG+LNIDVKDIDKV NINSEW V+VFR QPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 DRDVCIDNGVLNIDVKDIDKVANINSEWRVDVFRDQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRSTPN 431
           FA MY S PN
Sbjct: 421 FAHMYHSVPN 429

BLAST of Carg04144 vs. ExPASy TrEMBL
Match: A0A0A0K343 (Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G031700 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 2.8e-220
Identity = 364/437 (83.30%), Postives = 397/437 (90.85%), Query Frame = 0

Query: 1   MAINQKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFG 60
           MAINQKPR KD+AL+RFKLV+ VTLLG LLFMIL ETNL  NFA WSSD+I EDPY GFG
Sbjct: 1   MAINQKPRPKDAALKRFKLVVFVTLLGLLLFMILTETNLSWNFAAWSSDDIDEDPYLGFG 60

Query: 61  RFDSNDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVID 120
             DSN EL+LPKQ++LSIRLEKRNRLP RNLDLYP LA +HI I+LYVHNRPQYLRVV+D
Sbjct: 61  --DSNVELRLPKQSQLSIRLEKRNRLPPRNLDLYPKLANNHIAIVLYVHNRPQYLRVVVD 120

Query: 121 SLSKVTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDC 180
           SL+KV GISETLLIVSHDGYFEEMDKLVQ+IRFC VKQIFAPYSPHLFP+SFPGVSP DC
Sbjct: 121 SLAKVIGISETLLIVSHDGYFEEMDKLVQSIRFCQVKQIFAPYSPHLFPNSFPGVSPADC 180

Query: 181 IGKHYPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEE 240
            GK  P    +CRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGL+ET+G+SGHILFIEE
Sbjct: 181 KGKDDPK-AVHCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLKETQGMSGHILFIEE 240

Query: 241 DHFILPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNR 300
           DHFILPNAYRNLQLLV+LKPKKCPECYSVNLAPSDVKSRGEG+NFL+AERMGNIGYAFNR
Sbjct: 241 DHFILPNAYRNLQLLVTLKPKKCPECYSVNLAPSDVKSRGEGSNFLIAERMGNIGYAFNR 300

Query: 301 SVWKKIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQD 360
           +VW+KI+K+AKEFCFFDEYNWDITMWATV+PSFG PVYSLRGPW SA+HFGKCGLHQG  
Sbjct: 301 TVWRKIYKQAKEFCFFDEYNWDITMWATVYPSFGKPVYSLRGPWASAVHFGKCGLHQGHG 360

Query: 361 EGDVCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLN 420
           + D+CIDNG+LNIDVKDIDKV NINSEW V++FR QPGYGAGFRGWGGWGDERDRQLCLN
Sbjct: 361 DNDICIDNGVLNIDVKDIDKVANINSEWRVDIFRDQPGYGAGFRGWGGWGDERDRQLCLN 420

Query: 421 FAQMYRST---PNVSRS 435
           FA+MY S    PN+S S
Sbjct: 421 FARMYHSMSNGPNISHS 434

BLAST of Carg04144 vs. TAIR 10
Match: AT2G05320.1 (beta-1,2-N-acetylglucosaminyltransferase II )

HSP 1 Score: 567.0 bits (1460), Expect = 1.3e-161
Identity = 270/423 (63.83%), Postives = 327/423 (77.30%), Query Frame = 0

Query: 5   QKPRLKDSALRRFKLVILVTLLGFLLFMILMETNLFQNFAPWSSDNIREDPYAGFGRFDS 64
           +K RL+D+ L R  ++  VTL   L+ + +  T L  +      D + +D  A      +
Sbjct: 6   KKQRLRDTGLCRLGILFAVTLSIVLMLVSVPRTALNGSSIDDDLDGLDKDLEAKL----N 65

Query: 65  NDELKLPKQTELSIRLEKRNRLPLRNLDLYPNLAKDHIPILLYVHNRPQYLRVVIDSLSK 124
              L + +   +S+RL +RN    RN DL+P+LAKD + I+LYVHNR QY RV ++SLSK
Sbjct: 66  ASLLSVARGNRMSLRLHRRNHFSPRNTDLFPDLAKDRVVIVLYVHNRAQYFRVTVESLSK 125

Query: 125 VTGISETLLIVSHDGYFEEMDKLVQNIRFCSVKQIFAPYSPHLFPDSFPGVSPTDCIGKH 184
           V GISETLLIVSHDGYFEEM+++V++I+FC VKQIF+PYSPH++  SFPGV+  DC  K 
Sbjct: 126 VKGISETLLIVSHDGYFEEMNRIVESIKFCQVKQIFSPYSPHIYRTSFPGVTLNDCKNKG 185

Query: 185 YPMLTTNCRGNPDQYGNHRLPKIVSLKHHWWWMMNTVWDGLEETRGLSGHILFIEEDHFI 244
                 +C GNPDQYGNHR PKIVSLKHHWWWMMNTVWDGLEET+G  GHILFIEEDHF+
Sbjct: 186 -DEAKGHCEGNPDQYGNHRSPKIVSLKHHWWWMMNTVWDGLEETKGHEGHILFIEEDHFL 245

Query: 245 LPNAYRNLQLLVSLKPKKCPECYSVNLAPSDVKSRGEGNNFLVAERMGNIGYAFNRSVWK 304
            PNAYRN+Q L  LKP KCP+C++ NLAPSDVKSRGEG   LVAERMGN+GY+FNRSVW+
Sbjct: 246 FPNAYRNIQTLTRLKPAKCPDCFAANLAPSDVKSRGEGLESLVAERMGNVGYSFNRSVWE 305

Query: 305 KIHKKAKEFCFFDEYNWDITMWATVFPSFGSPVYSLRGPWTSAIHFGKCGLHQGQ-DEGD 364
            IH+KA+EFCFFD+YNWDITMWATVFPSFGSPVY+LRGP TSA+HFGKCGLHQG+ DEGD
Sbjct: 306 NIHQKAREFCFFDDYNWDITMWATVFPSFGSPVYTLRGPRTSAVHFGKCGLHQGRGDEGD 365

Query: 365 VCIDNGLLNIDVKDIDKVVNINSEWPVEVFRGQPGYGAGFRGWGGWGDERDRQLCLNFAQ 424
            CIDNG++NI+VK+ DKVVNI   W V V++ Q GY AGF GWGGWGD+RDR LCL+FA 
Sbjct: 366 -CIDNGVVNIEVKETDKVVNIKEGWGVRVYKHQAGYKAGFEGWGGWGDDRDRHLCLDFAT 422

Query: 425 MYR 427
           MYR
Sbjct: 426 MYR 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7011860.14.2e-263100.00Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial ... [more]
KAG6572221.11.2e-26299.77Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, partial ... [more]
XP_023554482.11.0e-26198.85alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbit... [more]
XP_022952309.15.2e-26199.08alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbit... [more]
XP_022969101.13.8e-25697.00alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [Cucurbit... [more]
Match NameE-valueIdentityDescription
Q9FT881.8e-16063.83Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Arabid... [more]
Q104691.1e-4833.05Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Homo s... [more]
Q093263.2e-4832.02Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Rattus... [more]
O190719.4e-4831.46Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Sus sc... [more]
Q921V52.1e-4731.46Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Mus mu... [more]
Match NameE-valueIdentityDescription
A0A6J1GLB72.5e-26199.08Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurb... [more]
A0A6J1HWS51.8e-25697.00Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucurb... [more]
A0A5A7UTF03.8e-22585.81Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi... [more]
A0A1S3CG233.8e-22585.81Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi... [more]
A0A0A0K3432.8e-22083.30Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase OS=Cucumi... [more]
Match NameE-valueIdentityDescription
AT2G05320.11.3e-16163.83beta-1,2-N-acetylglucosaminyltransferase II [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007754N-acetylglucosaminyltransferase IIPFAMPF05060MGAT2coord: 85..418
e-value: 9.5E-99
score: 330.7
IPR007754N-acetylglucosaminyltransferase IIPANTHERPTHR12871BETA-1,2-N-ACETYLGLUCOSAMINYLTRANSFERASE IIcoord: 6..425
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 101..361
e-value: 6.5E-28
score: 99.7
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 100..421
NoneNo IPR availablePANTHERPTHR12871:SF0ALPHA-1,6-MANNOSYL-GLYCOPROTEIN 2-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASEcoord: 6..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04144-RACarg04144-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009312 oligosaccharide biosynthetic process
biological_process GO:0006487 protein N-linked glycosylation
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0005795 Golgi stack
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008455 alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity
molecular_function GO:0046872 metal ion binding