Carg05840 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05840
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionBUB1 N-terminal domain-containing protein
LocationCarg_Chr16: 4765651 .. 4769322 (-)
RNA-Seq ExpressionCarg05840
SyntenyCarg05840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCACAGTGTATGCCTAACGGCCATGGCCGGCAACGACCTATTCTCTTCTTTGATTTCAGACATTAAGTCATACTCTGGCAAAGACCCTCTGCTTCCATGGCTCCGGTATGCCCTCAACAAATCACACTTCCATTTCTCTGCTTCTTCTTTGTCTCTCACACCTTATTCCTTGCCATTTTCTTGCAGAGGGATTCGGAAAATGAAAGACTCGTTGCCTACTCACGTTTTGAACGATAAGCTTCCTCGATTCTTGCAGAAATGCACCGAGAAGTTCGAGTCCGATCGGCGTTATAGGGACGATTTGCGGTATCTTCGCGTTTGGTTGCAGTTGGTAACTTGCTAATTCATCTCGGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTCACTTCCTTTCGGAGTTTGTATCTTTGAATTATTATTATTTTTCATCATATCAATTAGATTTTTCTCCTTTTTTTGTTGGAAAATAGTTAGAGTTTGATGTTTATAGCTCATAATGATTGATTTTGGGATTCAAACCTGATAATCAGATGGACTATGTGGATGATCCAAGAGCGCTCTTGAAAACCATGGAAGAGAAACGGATTGGGAGTAAATCTTCTTTGTTCTACCAAGCATACGCCCTGTACTATGAGAAGCTTAAGAAGTTTGATGATGCTGAGAAAATGTACCACTTAGGAGTGCAGAAGTAAGTTTTTTATTTGGAAGTTTGAATTCATTTTTATTTCATGTCTTGTTCCTCTATACGCTACGTGTGGCTTTATATTATGACTAATATGTTTAGTTTTGTGCTATTGTCTCCTGTTCTGTGAACCAAATTCAAATGAGTTAGCCATTTTTTATTCTGTGTCTATTGAGAATTGATGATTTAGAGATGCGTGATTGAGACAGAATAGTAAATGGATTGTGTGTGGTTTTGCCAGAGAATTGGAGGTAGTTGCGAATGACATCTAGTGTAAGATCTCACGTCTATTGGAGAGGAAAACGAAATATTTCTTATAAGGGCGTGGAAACCTCTTCCTAACATATGCGTCTTAAAACCATGAGGCTGGCGGCGATATATAATAGGTCAAAGTGGACAGTATCTGCTAGCGGCGGTGGGCTTAGGATGTTACAAATGGTATCAGAGCCAGGCATCGGGTGGTGTGCTAGCAAAGACGTTAGGCCTCCAAAAGAGGTGGATTATGAGATTCCACATCGGTTGGAGAAGGGAACGAAACATTCCTTATAAGGATGTGGATACCTCTCCCTAGCAGATTCGTTTTAAAACTGTGAGGCTGATGGCGATACGTAACAAACCAAAGCGGACAATATCCGTTAGCGGTAGGCTGGAGTTGTTACATCTAGTCATATGCTTTTTCTCAAAATTTTTCAATGAAAGTTTGAAAGACTAAATATTTTTGTGCTTTTATCTTTCTTCATAACATTATGATAACACCTTATGATTCAACAATCTTAGTATTTTAGTTTCATTATCCGTGCCCATGCCCATTGCGAAAATTGAATCTCTTGGAAGAAAAGAAAGTCGGGACAATTCGTCAACTTGGATAGCCTTGGCTAAGGGCAATACTAGTTGATAGTCTCAAGAGGAAATCATCTGAAACAAGGTAGAGACTAAATCTGTTAAATCAATGAGGCTTAACTGAGACAGAAGAATTCATATAGGCATATCTGTTCTCTCTCTTTTTTATTTGTGTAGTAGTTTTAACTTTCCAGTTTTCACAAGGATATTAAATTTGGGTTACACGTGTTACTCATGGCCTATTAGAAGTAGAAGGCCATTAGAAGTAGAAGGCACTCTGTTGGGATCTTCACGGACGGAAAAAGATTGCAATCAATTTGATAATGATCGTGTGACATCGCTTCTTCAGTCCGAACTCAGGAATCCCTTAGATATGATACAAAATGGATCTTGTTCTATCGTTGATAAGAGATTTCTCTATAAACAATATGAATTGGAGTTTGAAGAAGGAGAAGGAGAAGGGGTCCTCGACCCGCCACAGATAGAGGAAGATTTTTTCAATCACATAGTTTGAGCTCCTAGAATATGGCACCCTTGAGCTTTCTATTTGATTATATCGAAAGGCTCACTGAATCGGGTTTTTCCTATTTGGGCTAGGTTATTTCAGGCAAACGGATCATTTCTGATAAATAGGATGAGCTTTTAAGTAAATACGGTCATGATTTGCTTGTCATAACTCAACCTTTTGCCTTTTCCTTTGGGGATAACATTATTCTACTTTGTGAATTCTACCCATATTATTACATTTTTATCATTCTCATGGCTGTCTTCTATAATTTTTGTAGTTAACTTTTATATCTGCATCAATATCATTAAATCTTCTATTGAAGTACCTGCTATTAGTGTCAAGAAGTTAAGATGGAAAAACATGTTTTGGTTGTTGTACAGGCTTGCTGAACCTATTGATGAATTACAAAGGTCATATGAGCAGTTTCTTCGTCGCATGAAAAAACACAATTATAAGAAAGTTCAGGTGAGCCTTTTGTAATTATGCACTATACTTTCTGTCTCTTGTTGGGAAAATTCGGTTTGCTTTCTAAGGAACAACAGACATCCTGCAGCATCAGCATGGAAAAACTTCAAGGAGGCATCTGTCTGACAAAAGCACTCCTAATCAAGGTGATGCCGTGGTGAACAATAATGAGGATCAAAACAAAGGAATTTTTCAGAATGGGACTGAGAAGATGCCTCAAGATTCAGCAGCTGAAGCCAGGATGCTTAGGAGCGACGACACCGTCGTTGCGAAATTTGTAGATAATGCTATTTATGGGAAATCTGTAGCAGAAGATGCTTGTCACCACGGTTTGGTGGAACCAACTGTGAATATGAAAGAGGCAATGAATGCAATAAATAGTATGTTTTCTGAACCAATAGAGATGGCTCCTATAGGTAGGAGATCACATAGAAGCTATTCCAAGAAAGATTGCAACTTAAATGGTGGGTTTGAGGTGTTTGTTGATGAGAATCTTGATAAGAAACCTGAACTAGAAAAGCAGATGCCACAGCATGGTAGTATGACCGAACCATGCCAGCTCGAACAACCGCCTTTGATGATTTTTTTGGATGATGAAGTGGGTGGCGAGCTTGGCAACACAAGTTACGAAAAGGATGGATCAAAGTTGAGCGAATCACGATATGTCGTAGGAAGTTCTACGGCATCAGCATCACATTCAAAAGCGTTCATATTTCCTTGTGTGGACAATCCTGAAAATGTCAATGACGACGATTCTGATGTACAAGATCCTATTGAAGCGAAGTTTAGAGAAGATACAGTTGTTTGTAGGTTTGTTGGTACTGCCATTTCAAATGAGCCAGAGGTGGAAAACATTTGTCACCATGGCTTGGTAGAGCCAACAGTTAACCTGAAAGAAGCCATGGATGATATCAACAACATGTTTGGAAAGCCCATAGAATTTGTTAGGAGTAGGAGATCCAAGAAGCATGAAAATCCACCAGAAGTGCAGAATGATTTTGGTGGGTTCTCAATACTTGTTGATGATGATCTAGAGCAGCCTGCACAGGCTCAGCCACCAGTAAAGTCATCAAGAAAACCAAGAGACTGTGATTTGTTTGAGCCAACAGTGTTTACGAAAGAGGCCATGGATGATATCAACAAAATGTTTGGAATGCCTCATGATTAG

mRNA sequence

CTTCACAGTGTATGCCTAACGGCCATGGCCGGCAACGACCTATTCTCTTCTTTGATTTCAGACATTAAGTCATACTCTGGCAAAGACCCTCTGCTTCCATGGCTCCGAGGGATTCGGAAAATGAAAGACTCGTTGCCTACTCACGTTTTGAACGATAAGCTTCCTCGATTCTTGCAGAAATGCACCGAGAAGTTCGAGTCCGATCGGCGTTATAGGGACGATTTGCGGTATCTTCGCGTTTGGTTGCAGTTGATGGACTATGTGGATGATCCAAGAGCGCTCTTGAAAACCATGGAAGAGAAACGGATTGGGAGTAAATCTTCTTTGTTCTACCAAGCATACGCCCTGTACTATGAGAAGCTTAAGAAGTTTGATGATGCTGAGAAAATGTACCACTTAGGAGTGCAGAAGCTTGCTGAACCTATTGATGAATTACAAAGGTCATATGAGCAGTTTCTTCGTCGCATGAAAAAACACAATTATAAGAAAGTTCAGCATCAGCATGGAAAAACTTCAAGGAGGCATCTGTCTGACAAAAGCACTCCTAATCAAGGTGATGCCGTGGTGAACAATAATGAGGATCAAAACAAAGGAATTTTTCAGAATGGGACTGAGAAGATGCCTCAAGATTCAGCAGCTGAAGCCAGGATGCTTAGGAGCGACGACACCGTCGTTGCGAAATTTGTAGATAATGCTATTTATGGGAAATCTGTAGCAGAAGATGCTTGTCACCACGGTTTGGTGGAACCAACTGTGAATATGAAAGAGGCAATGAATGCAATAAATAGTATGTTTTCTGAACCAATAGAGATGGCTCCTATAGGTAGGAGATCACATAGAAGCTATTCCAAGAAAGATTGCAACTTAAATGGTGGGTTTGAGGTGTTTGTTGATGAGAATCTTGATAAGAAACCTGAACTAGAAAAGCAGATGCCACAGCATGGTAGTATGACCGAACCATGCCAGCTCGAACAACCGCCTTTGATGATTTTTTTGGATGATGAAGTGGGTGGCGAGCTTGGCAACACAAGTTACGAAAAGGATGGATCAAAGTTGAGCGAATCACGATATGTCGTAGGAAGTTCTACGGCATCAGCATCACATTCAAAAGCGTTCATATTTCCTTGTGTGGACAATCCTGAAAATGTCAATGACGACGATTCTGATGTACAAGATCCTATTGAAGCGAAGTTTAGAGAAGATACAGTTGTTTGTAGGTTTGTTGGTACTGCCATTTCAAATGAGCCAGAGGTGGAAAACATTTGTCACCATGGCTTGGTAGAGCCAACAGTTAACCTGAAAGAAGCCATGGATGATATCAACAACATGTTTGGAAAGCCCATAGAATTTGTTAGGAGTAGGAGATCCAAGAAGCATGAAAATCCACCAGAAGTGCAGAATGATTTTGGTGGGTTCTCAATACTTGTTGATGATGATCTAGAGCAGCCTGCACAGGCTCAGCCACCAGTAAAGTCATCAAGAAAACCAAGAGACTGTGATTTGTTTGAGCCAACAGTGTTTACGAAAGAGGCCATGGATGATATCAACAAAATGTTTGGAATGCCTCATGATTAG

Coding sequence (CDS)

ATGGCCGGCAACGACCTATTCTCTTCTTTGATTTCAGACATTAAGTCATACTCTGGCAAAGACCCTCTGCTTCCATGGCTCCGAGGGATTCGGAAAATGAAAGACTCGTTGCCTACTCACGTTTTGAACGATAAGCTTCCTCGATTCTTGCAGAAATGCACCGAGAAGTTCGAGTCCGATCGGCGTTATAGGGACGATTTGCGGTATCTTCGCGTTTGGTTGCAGTTGATGGACTATGTGGATGATCCAAGAGCGCTCTTGAAAACCATGGAAGAGAAACGGATTGGGAGTAAATCTTCTTTGTTCTACCAAGCATACGCCCTGTACTATGAGAAGCTTAAGAAGTTTGATGATGCTGAGAAAATGTACCACTTAGGAGTGCAGAAGCTTGCTGAACCTATTGATGAATTACAAAGGTCATATGAGCAGTTTCTTCGTCGCATGAAAAAACACAATTATAAGAAAGTTCAGCATCAGCATGGAAAAACTTCAAGGAGGCATCTGTCTGACAAAAGCACTCCTAATCAAGGTGATGCCGTGGTGAACAATAATGAGGATCAAAACAAAGGAATTTTTCAGAATGGGACTGAGAAGATGCCTCAAGATTCAGCAGCTGAAGCCAGGATGCTTAGGAGCGACGACACCGTCGTTGCGAAATTTGTAGATAATGCTATTTATGGGAAATCTGTAGCAGAAGATGCTTGTCACCACGGTTTGGTGGAACCAACTGTGAATATGAAAGAGGCAATGAATGCAATAAATAGTATGTTTTCTGAACCAATAGAGATGGCTCCTATAGGTAGGAGATCACATAGAAGCTATTCCAAGAAAGATTGCAACTTAAATGGTGGGTTTGAGGTGTTTGTTGATGAGAATCTTGATAAGAAACCTGAACTAGAAAAGCAGATGCCACAGCATGGTAGTATGACCGAACCATGCCAGCTCGAACAACCGCCTTTGATGATTTTTTTGGATGATGAAGTGGGTGGCGAGCTTGGCAACACAAGTTACGAAAAGGATGGATCAAAGTTGAGCGAATCACGATATGTCGTAGGAAGTTCTACGGCATCAGCATCACATTCAAAAGCGTTCATATTTCCTTGTGTGGACAATCCTGAAAATGTCAATGACGACGATTCTGATGTACAAGATCCTATTGAAGCGAAGTTTAGAGAAGATACAGTTGTTTGTAGGTTTGTTGGTACTGCCATTTCAAATGAGCCAGAGGTGGAAAACATTTGTCACCATGGCTTGGTAGAGCCAACAGTTAACCTGAAAGAAGCCATGGATGATATCAACAACATGTTTGGAAAGCCCATAGAATTTGTTAGGAGTAGGAGATCCAAGAAGCATGAAAATCCACCAGAAGTGCAGAATGATTTTGGTGGGTTCTCAATACTTGTTGATGATGATCTAGAGCAGCCTGCACAGGCTCAGCCACCAGTAAAGTCATCAAGAAAACCAAGAGACTGTGATTTGTTTGAGCCAACAGTGTTTACGAAAGAGGCCATGGATGATATCAACAAAATGTTTGGAATGCCTCATGATTAG

Protein sequence

MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESDRRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAEKMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAVVNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLVEPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELEKQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASHSKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQPPVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPHD
Homology
BLAST of Carg05840 vs. NCBI nr
Match: KAG7015406.1 (Mitotic spindle checkpoint protein BUBR1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1043.1 bits (2696), Expect = 7.9e-301
Identity = 516/516 (100.00%), Postives = 516/516 (100.00%), Query Frame = 0

Query: 1   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60
           MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD
Sbjct: 1   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60

Query: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120
           RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE
Sbjct: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120

Query: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180
           KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV
Sbjct: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180

Query: 181 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 240
           VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV
Sbjct: 181 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 240

Query: 241 EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE 300
           EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE
Sbjct: 241 EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE 300

Query: 301 KQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASH 360
           KQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASH
Sbjct: 301 KQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASH 360

Query: 361 SKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 420
           SKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE
Sbjct: 361 SKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 420

Query: 421 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 480
           PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP
Sbjct: 421 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 480

Query: 481 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPHD 517
           PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPHD
Sbjct: 481 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPHD 516

BLAST of Carg05840 vs. NCBI nr
Match: XP_022929208.1 (uncharacterized protein LOC111435869 [Cucurbita moschata])

HSP 1 Score: 1030.0 bits (2662), Expect = 6.9e-297
Identity = 510/516 (98.84%), Postives = 513/516 (99.42%), Query Frame = 0

Query: 1   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60
           MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD
Sbjct: 3   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 62

Query: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120
           RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE
Sbjct: 63  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 122

Query: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180
           KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKST NQGDAV
Sbjct: 123 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTSNQGDAV 182

Query: 181 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 240
           VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV
Sbjct: 183 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 242

Query: 241 EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE 300
           EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE
Sbjct: 243 EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE 302

Query: 301 KQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASH 360
           KQMPQHGSMTEPCQLEQPPLMIF+DDEVGGELGNTSYEKDGSKLS+SRYVVGSSTASAS 
Sbjct: 303 KQMPQHGSMTEPCQLEQPPLMIFVDDEVGGELGNTSYEKDGSKLSDSRYVVGSSTASASR 362

Query: 361 SKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 420
           SKAF+FPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE
Sbjct: 363 SKAFVFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 422

Query: 421 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 480
           PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP
Sbjct: 423 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 482

Query: 481 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPHD 517
           PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMP D
Sbjct: 483 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPLD 518

BLAST of Carg05840 vs. NCBI nr
Match: KAG6577315.1 (Mitotic spindle checkpoint protein BUBR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1016.5 bits (2627), Expect = 7.9e-293
Identity = 507/516 (98.26%), Postives = 508/516 (98.45%), Query Frame = 0

Query: 1   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60
           MAGNDLFSSLI+DIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD
Sbjct: 1   MAGNDLFSSLIADIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60

Query: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120
           RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE
Sbjct: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120

Query: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180
           KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV
Sbjct: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180

Query: 181 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 240
           VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV
Sbjct: 181 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 240

Query: 241 EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE 300
           EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE
Sbjct: 241 EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE 300

Query: 301 KQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASH 360
           KQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASAS 
Sbjct: 301 KQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASR 360

Query: 361 SKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 420
           SKAFIFPCVDNPENVNDDDSD      AKFREDTVVCRFVGTAISNEPEVENICHHGLVE
Sbjct: 361 SKAFIFPCVDNPENVNDDDSD------AKFREDTVVCRFVGTAISNEPEVENICHHGLVE 420

Query: 421 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 480
           PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP
Sbjct: 421 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 480

Query: 481 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPHD 517
           PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMP D
Sbjct: 481 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPLD 510

BLAST of Carg05840 vs. NCBI nr
Match: XP_023552017.1 (uncharacterized protein LOC111809814 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1013.4 bits (2619), Expect = 6.7e-292
Identity = 500/516 (96.90%), Postives = 508/516 (98.45%), Query Frame = 0

Query: 1   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60
           MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD
Sbjct: 3   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 62

Query: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120
           RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDA+
Sbjct: 63  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAD 122

Query: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180
           KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDK T NQGDAV
Sbjct: 123 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKITSNQGDAV 182

Query: 181 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 240
           VNNNEDQNKGIFQNGTEKMPQDSAAE+RML+SDDTVVAKFVDNAIYGKSVAEDACHHGLV
Sbjct: 183 VNNNEDQNKGIFQNGTEKMPQDSAAESRMLKSDDTVVAKFVDNAIYGKSVAEDACHHGLV 242

Query: 241 EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE 300
           EPTVNMKEAMNAINSMFSEPIEMAP+GRRSHRS+S  DCNLNGGFEVFVDENLDKKPELE
Sbjct: 243 EPTVNMKEAMNAINSMFSEPIEMAPVGRRSHRSHSNNDCNLNGGFEVFVDENLDKKPELE 302

Query: 301 KQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASH 360
           KQMPQHGSMTEPCQLEQPPLMIF+DDE GGELGNTSYEKDGSKLSESRYVVGSS ASAS 
Sbjct: 303 KQMPQHGSMTEPCQLEQPPLMIFVDDEAGGELGNTSYEKDGSKLSESRYVVGSSMASASR 362

Query: 361 SKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 420
           SKAF+FPCVDNPENV+DDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE
Sbjct: 363 SKAFVFPCVDNPENVDDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 422

Query: 421 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 480
           PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP
Sbjct: 423 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 482

Query: 481 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPHD 517
           PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMP D
Sbjct: 483 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPLD 518

BLAST of Carg05840 vs. NCBI nr
Match: XP_022984556.1 (uncharacterized protein LOC111482816 [Cucurbita maxima])

HSP 1 Score: 1000.3 bits (2585), Expect = 5.9e-288
Identity = 499/516 (96.71%), Postives = 504/516 (97.67%), Query Frame = 0

Query: 1   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60
           MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD
Sbjct: 3   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 62

Query: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120
           RRYR+DLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE
Sbjct: 63  RRYRNDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 122

Query: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180
           KMYH GVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKST NQGDAV
Sbjct: 123 KMYHFGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTSNQGDAV 182

Query: 181 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 240
           VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV
Sbjct: 183 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 242

Query: 241 EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE 300
           EPTVNMKEAMNAINSMFSEPIEMA IGRRSHRS+    CNLNGGFEVF DENLDKKPELE
Sbjct: 243 EPTVNMKEAMNAINSMFSEPIEMATIGRRSHRSH----CNLNGGFEVFADENLDKKPELE 302

Query: 301 KQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASH 360
           KQMPQHGSMTEPCQLEQPPLMIF+DDE GGELGNTSYEKDGSKLSESRYVVGSSTASAS 
Sbjct: 303 KQMPQHGSMTEPCQLEQPPLMIFVDDEAGGELGNTSYEKDGSKLSESRYVVGSSTASASG 362

Query: 361 SKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 420
           SKAF+FPCV NPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE
Sbjct: 363 SKAFVFPCVYNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 422

Query: 421 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 480
           PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQN+FGGFSILVDDDLEQPAQAQP
Sbjct: 423 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNNFGGFSILVDDDLEQPAQAQP 482

Query: 481 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPHD 517
           PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMP D
Sbjct: 483 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPLD 514

BLAST of Carg05840 vs. ExPASy Swiss-Prot
Match: Q54CV5 (Probable inactive serine/threonine-protein kinase bub1 OS=Dictyostelium discoideum OX=44689 GN=bub1 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.3e-19
Identity = 54/165 (32.73%), Postives = 93/165 (56.36%), Query Frame = 0

Query: 14  IKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESDRRYRDDLRYLRVW 73
           I  Y G+DP+  WL+ I+ ++ S P   + ++L   L++CT  F S  +Y++D RYLR+W
Sbjct: 51  INEYQGEDPIDNWLKYIKWVQQSYPGGNMKEQLIVLLERCTRLFLSTEKYKNDPRYLRIW 110

Query: 74  LQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAEKMYHLGVQKLAEP 133
           +   D   DP  +   +E +RIG   SL Y+A A+ YE    ++ A+K +  G+++ A+P
Sbjct: 111 ITYADMCRDPIEVFSFLEVQRIGFYLSLLYEARAIVYENKGNYEQADKSFKQGIERKAQP 170

Query: 134 IDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGD 179
           I+ LQ+ +  F RR+      + QHQ  +  ++  +D+    Q D
Sbjct: 171 IERLQQKHLDFERRLIARLKHQQQHQQLQQQQQQQNDQLEQQQLD 215

BLAST of Carg05840 vs. ExPASy Swiss-Prot
Match: O59767 (Mitotic spindle checkpoint component mad3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mad3 PE=1 SV=2)

HSP 1 Score: 95.1 bits (235), Expect = 2.4e-18
Identity = 48/133 (36.09%), Postives = 75/133 (56.39%), Query Frame = 0

Query: 16  SYSGKDPLLPWLRGIRKMKDSLPT-HVLNDKLPRFLQKCTEKFESDRRYRDDLRYLRVWL 75
           S S  DPL  W+  I+   D+ P        L   L++CT +F  +  Y+DD+RYLR+W+
Sbjct: 65  SESLDDPLQVWIDYIKWTLDNFPQGETKTSGLVTLLERCTREFVRNPLYKDDVRYLRIWM 124

Query: 76  QLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAEKMYHLGVQKLAEPI 135
           Q ++Y+D+P  L   +    IG +SS+FY+ YA Y+E    F  A+++Y  G +  A+P 
Sbjct: 125 QYVNYIDEPVELFSFLAHHHIGQESSIFYEEYANYFESRGLFQKADEVYQKGKRMKAKPF 184

Query: 136 DELQRSYEQFLRR 148
              Q+ Y+QF  R
Sbjct: 185 LRFQQKYQQFTHR 197

BLAST of Carg05840 vs. ExPASy Swiss-Prot
Match: O22806 (Mitotic spindle checkpoint protein BUBR1 OS=Arabidopsis thaliana OX=3702 GN=BUBR1 PE=1 SV=2)

HSP 1 Score: 93.6 bits (231), Expect = 7.1e-18
Identity = 61/204 (29.90%), Postives = 105/204 (51.47%), Query Frame = 0

Query: 9   SLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESDRRYRDDLR 68
           +LI  I  Y G DPL PW+  I+ ++++ P       L    ++C  KF    RY+DDLR
Sbjct: 68  NLIEAIDEYEGDDPLSPWIECIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERYKDDLR 127

Query: 69  YLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAEKMYHLGVQ 128
           YL+VWL+  ++  D   + K +E   IG   +++Y AYAL+ E   K   A ++++LG+ 
Sbjct: 128 YLKVWLEYAEHCADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGIS 187

Query: 129 KLAEPIDELQRSYEQFL-RRMKKHN----YKKVQHQHGKTSRRHLSDKSTPNQGDAVVNN 188
           + A+P+++L  +Y++F+ R M++ N      K  +     S   L  +   N     + +
Sbjct: 188 RDAKPVEKLNDAYKKFMVRTMRRSNTADEEPKENNDLPSRSFGTLLSRGDNNARRQALGS 247

Query: 189 NEDQNKGIFQNGTEKMPQDSAAEA 208
           +  Q K +  N + K P    A+A
Sbjct: 248 SNPQAKKLKPNQSSKTPFAIYADA 271

BLAST of Carg05840 vs. ExPASy Swiss-Prot
Match: F4IVI0 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana OX=3702 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.8e-13
Identity = 43/131 (32.82%), Postives = 70/131 (53.44%), Query Frame = 0

Query: 21  DPLLPWLRGIR-KMKDSLPTHVLNDKLPRFLQKCTEKFESDRRYRDDLRYLRVWLQLMDY 80
           DPL PWL  I+  M+D          L + L  C   ++ D RYR+DLR+L++W   ++ 
Sbjct: 11  DPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLYLEG 70

Query: 81  VDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAEKMYHLGVQKLAEPIDELQR 140
            +D   + + +EE  I    SL Y+ YA++ E    +  A  +Y  G+ + AEP D L+ 
Sbjct: 71  REDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDRLKE 130

Query: 141 SYEQFLRRMKK 151
           ++  FL+R+ K
Sbjct: 131 AHSLFLQRISK 141

BLAST of Carg05840 vs. ExPASy Swiss-Prot
Match: Q9Z1S0 (Mitotic checkpoint serine/threonine-protein kinase BUB1 beta OS=Mus musculus OX=10090 GN=Bub1b PE=1 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 3.1e-13
Identity = 40/142 (28.17%), Postives = 76/142 (53.52%), Query Frame = 0

Query: 12  SDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESDRRYRDDLRYLR 71
           S+I+ YSG DPL  W R I   + + P       +   +++  E  + + RY +D R+L 
Sbjct: 58  SEIRFYSGDDPLDVWDRYINWTEQNYPQGGKESNMSALVERAIEALQGETRYYNDPRFLS 117

Query: 72  VWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAEKMYHLGVQKLA 131
           +W++L    ++P  +   ++ + IG   + FY ++A  YE  + F  A+ ++  G+++ A
Sbjct: 118 LWIKLGHLCNEPLDMYSYLQSQGIGVSLAQFYISWAEEYEARENFKKADIIFQEGIERKA 177

Query: 132 EPIDELQRSYEQFLRRMKKHNY 154
           EP+D LQ  + QF  R+ +  +
Sbjct: 178 EPLDRLQSQHRQFQSRVSRQAF 199

BLAST of Carg05840 vs. ExPASy TrEMBL
Match: A0A6J1ERG0 (uncharacterized protein LOC111435869 OS=Cucurbita moschata OX=3662 GN=LOC111435869 PE=4 SV=1)

HSP 1 Score: 1030.0 bits (2662), Expect = 3.4e-297
Identity = 510/516 (98.84%), Postives = 513/516 (99.42%), Query Frame = 0

Query: 1   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60
           MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD
Sbjct: 3   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 62

Query: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120
           RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE
Sbjct: 63  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 122

Query: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180
           KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKST NQGDAV
Sbjct: 123 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTSNQGDAV 182

Query: 181 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 240
           VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV
Sbjct: 183 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 242

Query: 241 EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE 300
           EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE
Sbjct: 243 EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE 302

Query: 301 KQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASH 360
           KQMPQHGSMTEPCQLEQPPLMIF+DDEVGGELGNTSYEKDGSKLS+SRYVVGSSTASAS 
Sbjct: 303 KQMPQHGSMTEPCQLEQPPLMIFVDDEVGGELGNTSYEKDGSKLSDSRYVVGSSTASASR 362

Query: 361 SKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 420
           SKAF+FPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE
Sbjct: 363 SKAFVFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 422

Query: 421 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 480
           PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP
Sbjct: 423 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 482

Query: 481 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPHD 517
           PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMP D
Sbjct: 483 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPLD 518

BLAST of Carg05840 vs. ExPASy TrEMBL
Match: A0A6J1J5L0 (uncharacterized protein LOC111482816 OS=Cucurbita maxima OX=3661 GN=LOC111482816 PE=4 SV=1)

HSP 1 Score: 1000.3 bits (2585), Expect = 2.8e-288
Identity = 499/516 (96.71%), Postives = 504/516 (97.67%), Query Frame = 0

Query: 1   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60
           MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD
Sbjct: 3   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 62

Query: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120
           RRYR+DLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE
Sbjct: 63  RRYRNDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 122

Query: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180
           KMYH GVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKST NQGDAV
Sbjct: 123 KMYHFGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTSNQGDAV 182

Query: 181 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 240
           VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV
Sbjct: 183 VNNNEDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLV 242

Query: 241 EPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGGFEVFVDENLDKKPELE 300
           EPTVNMKEAMNAINSMFSEPIEMA IGRRSHRS+    CNLNGGFEVF DENLDKKPELE
Sbjct: 243 EPTVNMKEAMNAINSMFSEPIEMATIGRRSHRSH----CNLNGGFEVFADENLDKKPELE 302

Query: 301 KQMPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASH 360
           KQMPQHGSMTEPCQLEQPPLMIF+DDE GGELGNTSYEKDGSKLSESRYVVGSSTASAS 
Sbjct: 303 KQMPQHGSMTEPCQLEQPPLMIFVDDEAGGELGNTSYEKDGSKLSESRYVVGSSTASASG 362

Query: 361 SKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 420
           SKAF+FPCV NPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE
Sbjct: 363 SKAFVFPCVYNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPEVENICHHGLVE 422

Query: 421 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNDFGGFSILVDDDLEQPAQAQP 480
           PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQN+FGGFSILVDDDLEQPAQAQP
Sbjct: 423 PTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQNNFGGFSILVDDDLEQPAQAQP 482

Query: 481 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPHD 517
           PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMP D
Sbjct: 483 PVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPLD 514

BLAST of Carg05840 vs. ExPASy TrEMBL
Match: A0A1S3CCD9 (uncharacterized protein LOC103499203 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499203 PE=4 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.3e-240
Identity = 431/534 (80.71%), Postives = 467/534 (87.45%), Query Frame = 0

Query: 1   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60
           MA NDLFSSLISDIKSYSGKDPLLPWLRGIRK+KDSLP+HVLNDKLPRFLQKCTEKFESD
Sbjct: 3   MASNDLFSSLISDIKSYSGKDPLLPWLRGIRKLKDSLPSHVLNDKLPRFLQKCTEKFESD 62

Query: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120
           RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEK+IG+KSSLFYQAYALYYEKLKKFDDA+
Sbjct: 63  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKQIGTKSSLFYQAYALYYEKLKKFDDAD 122

Query: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180
           KMYHLGVQKLA+PIDELQRSY+QFL RMK+HNYK+VQHQHG+TSRRHLSDKS  NQ DA 
Sbjct: 123 KMYHLGVQKLAQPIDELQRSYQQFLHRMKRHNYKRVQHQHGRTSRRHLSDKSISNQDDAE 182

Query: 181 VNNNEDQNKGIFQNG------------TEKMPQDSAA----EARMLRSDDTVVAKFVDNA 240
            NN + Q   IF+NG            TEK+PQDS      E+RM++SDDT+V +FVD A
Sbjct: 183 KNNIKVQEGEIFENGPLAATLSDTRSETEKLPQDSGGRETDESRMVKSDDTIVVRFVDRA 242

Query: 241 IYGKSVAEDACHHGLVEPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGG 300
           IYGKSVAEDACHHGLVEPTVNMKEAMN INSMFSEPIEM PIGRRS  S+SKKDCNLNGG
Sbjct: 243 IYGKSVAEDACHHGLVEPTVNMKEAMNTINSMFSEPIEMPPIGRRSCGSHSKKDCNLNGG 302

Query: 301 FEVFVDENLDKKPELEKQ----MPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKD 360
           FEVFVDE  DK+ ELEKQ    +PQHGS++EP +LEQP LMIF+DDE GGE+GNTS EK 
Sbjct: 303 FEVFVDEKHDKEIELEKQKGCTIPQHGSISEPGKLEQPHLMIFVDDESGGEIGNTSIEKV 362

Query: 361 GSKLSESRYVVGSSTASASHSKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFV 420
           G KLS+SR VVG ST SAS SKAF+FPCVDN EN+ DDDSD QDP++AKFREDT+VCRFV
Sbjct: 363 GLKLSKSRDVVGGSTTSASRSKAFVFPCVDNLENM-DDDSDAQDPMQAKFREDTIVCRFV 422

Query: 421 GTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQND 480
           GTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVR+RRSKKHE PPEVQN+
Sbjct: 423 GTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRTRRSKKHEKPPEVQNN 482

Query: 481 FGGFSILVDDDLEQPAQAQPPVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMP 515
           FGGFSIL DD+L +PAQAQ       K RDCDLFEPTVFTKEAMDDINKMFGMP
Sbjct: 483 FGGFSILPDDELLEPAQAQ-------KSRDCDLFEPTVFTKEAMDDINKMFGMP 528

BLAST of Carg05840 vs. ExPASy TrEMBL
Match: A0A5D3D6J0 (Mad3_BUB1_I domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00980 PE=4 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.3e-240
Identity = 431/534 (80.71%), Postives = 467/534 (87.45%), Query Frame = 0

Query: 1   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60
           MA NDLFSSLISDIKSYSGKDPLLPWLRGIRK+KDSLP+HVLNDKLPRFLQKCTEKFESD
Sbjct: 3   MASNDLFSSLISDIKSYSGKDPLLPWLRGIRKLKDSLPSHVLNDKLPRFLQKCTEKFESD 62

Query: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120
           RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEK+IG+KSSLFYQAYALYYEKLKKFDDA+
Sbjct: 63  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKQIGTKSSLFYQAYALYYEKLKKFDDAD 122

Query: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180
           KMYHLGVQKLA+PIDELQRSY+QFL RMK+HNYK+VQHQHG+TSRRHLSDKS  NQ DA 
Sbjct: 123 KMYHLGVQKLAQPIDELQRSYQQFLHRMKRHNYKRVQHQHGRTSRRHLSDKSISNQDDAE 182

Query: 181 VNNNEDQNKGIFQNG------------TEKMPQDSAA----EARMLRSDDTVVAKFVDNA 240
            NN + Q   IF+NG            TEK+PQDS      E+RM++SDDT+V +FVD A
Sbjct: 183 KNNIKVQEGEIFENGPLAATLSDTRSETEKLPQDSGGRETDESRMVKSDDTIVVRFVDRA 242

Query: 241 IYGKSVAEDACHHGLVEPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGG 300
           IYGKSVAEDACHHGLVEPTVNMKEAMN INSMFSEPIEM PIGRRS  S+SKKDCNLNGG
Sbjct: 243 IYGKSVAEDACHHGLVEPTVNMKEAMNTINSMFSEPIEMPPIGRRSCGSHSKKDCNLNGG 302

Query: 301 FEVFVDENLDKKPELEKQ----MPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKD 360
           FEVFVDE  DK+ ELEKQ    +PQHGS++EP +LEQP LMIF+DDE GGE+GNTS EK 
Sbjct: 303 FEVFVDEKHDKEIELEKQKGCTIPQHGSISEPGKLEQPHLMIFVDDESGGEIGNTSIEKV 362

Query: 361 GSKLSESRYVVGSSTASASHSKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFV 420
           G KLS+SR VVG ST SAS SKAF+FPCVDN EN+ DDDSD QDP++AKFREDT+VCRFV
Sbjct: 363 GLKLSKSRDVVGGSTTSASRSKAFVFPCVDNLENM-DDDSDAQDPMQAKFREDTIVCRFV 422

Query: 421 GTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQND 480
           GTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVR+RRSKKHE PPEVQN+
Sbjct: 423 GTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRTRRSKKHEKPPEVQNN 482

Query: 481 FGGFSILVDDDLEQPAQAQPPVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMP 515
           FGGFSIL DD+L +PAQAQ       K RDCDLFEPTVFTKEAMDDINKMFGMP
Sbjct: 483 FGGFSILPDDELLEPAQAQ-------KSRDCDLFEPTVFTKEAMDDINKMFGMP 528

BLAST of Carg05840 vs. ExPASy TrEMBL
Match: A0A5A7UEU2 (Mad3_BUB1_I domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold190G001480 PE=4 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.9e-240
Identity = 430/534 (80.52%), Postives = 467/534 (87.45%), Query Frame = 0

Query: 1   MAGNDLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESD 60
           MA NDLFSSLISDIKSYSGKDPLLPWLRGIRK+KDSLP+HVLNDKLPRFLQKCTEKFESD
Sbjct: 3   MASNDLFSSLISDIKSYSGKDPLLPWLRGIRKLKDSLPSHVLNDKLPRFLQKCTEKFESD 62

Query: 61  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAE 120
           RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEK+IG+KSSLFYQAYALYYEKLKKFDDA+
Sbjct: 63  RRYRDDLRYLRVWLQLMDYVDDPRALLKTMEEKQIGTKSSLFYQAYALYYEKLKKFDDAD 122

Query: 121 KMYHLGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAV 180
           KMYHLGVQKLA+PIDELQRSY+QFL RMK+HNYK+VQHQHG+TSRRHLSDKS  NQ DA 
Sbjct: 123 KMYHLGVQKLAQPIDELQRSYQQFLHRMKRHNYKRVQHQHGRTSRRHLSDKSISNQDDAE 182

Query: 181 VNNNEDQNKGIFQNG------------TEKMPQDSAA----EARMLRSDDTVVAKFVDNA 240
            NN + Q   IF+NG            TEK+PQDS      E+RM++SDDT+V +FVD A
Sbjct: 183 KNNIKVQEGEIFENGPLAATLSDTRSETEKLPQDSGGRETDESRMVKSDDTIVVRFVDRA 242

Query: 241 IYGKSVAEDACHHGLVEPTVNMKEAMNAINSMFSEPIEMAPIGRRSHRSYSKKDCNLNGG 300
           IYGKSVAEDACHHGLVEPTVNMKEAMN INSMFSEPIEM PIGRRS  S+SKKDCNLNGG
Sbjct: 243 IYGKSVAEDACHHGLVEPTVNMKEAMNTINSMFSEPIEMPPIGRRSCGSHSKKDCNLNGG 302

Query: 301 FEVFVDENLDKKPELEKQ----MPQHGSMTEPCQLEQPPLMIFLDDEVGGELGNTSYEKD 360
           FEVFVDE  DK+ ELEKQ    +PQHGS++EP +LEQP LMIF+DDE GGE+GNTS EK 
Sbjct: 303 FEVFVDEKHDKEIELEKQKGCTIPQHGSISEPGKLEQPHLMIFVDDESGGEIGNTSIEKV 362

Query: 361 GSKLSESRYVVGSSTASASHSKAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFV 420
           G KLS+SR VVG ST SAS SKAF+FPCVDN EN+ DDDSD QDP++AKFREDT+VCRFV
Sbjct: 363 GLKLSKSRDVVGGSTTSASRSKAFVFPCVDNLENM-DDDSDAQDPMQAKFREDTIVCRFV 422

Query: 421 GTAISNEPEVENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRSRRSKKHENPPEVQND 480
           GTAISNEPE+ENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVR+RRSKKHE PPEVQN+
Sbjct: 423 GTAISNEPEMENICHHGLVEPTVNLKEAMDDINNMFGKPIEFVRTRRSKKHEKPPEVQNN 482

Query: 481 FGGFSILVDDDLEQPAQAQPPVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMP 515
           FGGFSIL DD+L +PAQAQ       K RDCDLFEPTVFTKEAMDDINKMFGMP
Sbjct: 483 FGGFSILPDDELLEPAQAQ-------KSRDCDLFEPTVFTKEAMDDINKMFGMP 528

BLAST of Carg05840 vs. TAIR 10
Match: AT5G05510.1 (Mad3/BUB1 homology region 1 )

HSP 1 Score: 462.6 bits (1189), Expect = 4.1e-130
Identity = 271/523 (51.82%), Postives = 337/523 (64.44%), Query Frame = 0

Query: 5   DLFSSLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESDRRYR 64
           +L +SLI DIKSYSGKD LLPW+RG++KMK+SLP+ +LN+KLPRFLQKC E FESD+RY+
Sbjct: 10  NLLASLIVDIKSYSGKDHLLPWIRGVKKMKESLPSQILNEKLPRFLQKCAESFESDKRYK 69

Query: 65  DDLRYLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAEKMYH 124
           +D RY+RVWLQLMD+VDDPRALL+TME K IG+K SLFYQAYAL+YEK+K+F+DAEKMY 
Sbjct: 70  NDSRYIRVWLQLMDFVDDPRALLRTMEAKSIGTKRSLFYQAYALHYEKMKRFEDAEKMYR 129

Query: 125 LGVQKLAEPIDELQRSYEQFLRRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGDAVVNNN 184
           LGVQ LAEP+DELQ+SY QF+ RM++H  KK Q Q  K S +H                 
Sbjct: 130 LGVQNLAEPMDELQKSYLQFVTRMERHKKKKTQRQEQKLSGKH----------------- 189

Query: 185 EDQNKGIFQNGTEKMPQDSAAEARMLRSDDTVVAKFVDNAIYGKSVAEDACHHGLVEPTV 244
                        K+ +         R     V  FVD AI GK  AE+ACHHGLV+PT+
Sbjct: 190 ------------HKVDE---------RQQHEPVLNFVDKAIVGKPEAENACHHGLVDPTI 249

Query: 245 NMKEAMNAINSMFSEPIEMAPIGRRS-HRSYSKKDCNLNGGFEVFVDENLDKKPELEKQM 304
           NMKEAMN IN+MF EPIE AP+ RRS  RS +K++   N  FEVF+DENL+ +     + 
Sbjct: 250 NMKEAMNTINNMFKEPIETAPLQRRSRQRSQNKENQGCNNSFEVFLDENLECETGTSGKA 309

Query: 305 PQHGSMTEPC--QLEQPPLMIFLDDEVGGELGNTSYEKDGSKLSESRYVVGSSTASASHS 364
               + T  C  Q  Q    IF+DDE   E   T+ E D                 A  +
Sbjct: 310 K---TSTTQCGSQPNQESFEIFIDDENTDE---TADEND----------------EAGKA 369

Query: 365 KAFIFPCVDNPENVNDDDSDVQDPIEAKFREDTVVCRFVGTAISNEPE-VENICHHGLVE 424
             F+ P   +PE  + +++D   P  A+FREDTVV RFVG+ IS+EPE VEN CHHGLV+
Sbjct: 370 FVFLLPRDHSPE--SSEEADRNTPPRARFREDTVVRRFVGSTISDEPEAVENACHHGLVD 429

Query: 425 PTVNLKEAMDDINNMFGKPIEFVRSRRSK-KHENPPEVQ-NDFGGFSILVDDDLEQPAQ- 484
           PTVNLKEAM+DINNMFG+PI FVR  RSK K +   E + N   GFSIL DDD E+  Q 
Sbjct: 430 PTVNLKEAMEDINNMFGEPINFVRPNRSKNKGKAVVETKPNPAAGFSILEDDDEEEAEQE 470

Query: 485 ----AQPPVKSSRKPRDCDLFEPTVFTKEAMDDINKMFGMPHD 517
               +QP  +   K  + +LFEPTV TK A+D+INK+F MP D
Sbjct: 490 HQGKSQPTQRLPSKSDERELFEPTVCTKVALDEINKLFAMPMD 470

BLAST of Carg05840 vs. TAIR 10
Match: AT2G33560.2 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 94.7 bits (234), Expect = 2.3e-19
Identity = 61/205 (29.76%), Postives = 106/205 (51.71%), Query Frame = 0

Query: 9   SLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESDRRYRDDLR 68
           +LI  I  Y G DPL PW+  I+ ++++ P       L    ++C  KF    RY+DDLR
Sbjct: 68  NLIEAIDEYEGDDPLSPWIECIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERYKDDLR 127

Query: 69  YLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAEKMYHLGVQ 128
           YL+VWL+  ++  D   + K +E   IG   +++Y AYAL+ E   K   A ++++LG+ 
Sbjct: 128 YLKVWLEYAEHCADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGIS 187

Query: 129 KLAEPIDELQRSYEQFL-RRMKKHNYKKVQHQHGKTSRRHLSDKSTPNQGD-----AVVN 188
           + A+P+++L  +Y++F+ R M++ N    Q           S  +  ++GD       + 
Sbjct: 188 RDAKPVEKLNDAYKKFMVRTMRRSNTADEQEPKENNDLPSRSFGTLLSRGDNNARRQALG 247

Query: 189 NNEDQNKGIFQNGTEKMPQDSAAEA 208
           ++  Q K +  N + K P    A+A
Sbjct: 248 SSNPQAKKLKPNQSSKTPFAIYADA 272

BLAST of Carg05840 vs. TAIR 10
Match: AT2G33560.1 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 93.6 bits (231), Expect = 5.0e-19
Identity = 61/204 (29.90%), Postives = 105/204 (51.47%), Query Frame = 0

Query: 9   SLISDIKSYSGKDPLLPWLRGIRKMKDSLPTHVLNDKLPRFLQKCTEKFESDRRYRDDLR 68
           +LI  I  Y G DPL PW+  I+ ++++ P       L    ++C  KF    RY+DDLR
Sbjct: 68  NLIEAIDEYEGDDPLSPWIECIKWVQEAFPPGGECSGLLVIYEQCVRKFWHSERYKDDLR 127

Query: 69  YLRVWLQLMDYVDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAEKMYHLGVQ 128
           YL+VWL+  ++  D   + K +E   IG   +++Y AYAL+ E   K   A ++++LG+ 
Sbjct: 128 YLKVWLEYAEHCADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGIS 187

Query: 129 KLAEPIDELQRSYEQFL-RRMKKHN----YKKVQHQHGKTSRRHLSDKSTPNQGDAVVNN 188
           + A+P+++L  +Y++F+ R M++ N      K  +     S   L  +   N     + +
Sbjct: 188 RDAKPVEKLNDAYKKFMVRTMRRSNTADEEPKENNDLPSRSFGTLLSRGDNNARRQALGS 247

Query: 189 NEDQNKGIFQNGTEKMPQDSAAEA 208
           +  Q K +  N + K P    A+A
Sbjct: 248 SNPQAKKLKPNQSSKTPFAIYADA 271

BLAST of Carg05840 vs. TAIR 10
Match: AT2G20635.1 (ATP binding;protein kinases;protein serine/threonine kinases )

HSP 1 Score: 79.0 bits (193), Expect = 1.3e-14
Identity = 43/131 (32.82%), Postives = 70/131 (53.44%), Query Frame = 0

Query: 21  DPLLPWLRGIR-KMKDSLPTHVLNDKLPRFLQKCTEKFESDRRYRDDLRYLRVWLQLMDY 80
           DPL PWL  I+  M+D          L + L  C   ++ D RYR+DLR+L++W   ++ 
Sbjct: 11  DPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLYLEG 70

Query: 81  VDDPRALLKTMEEKRIGSKSSLFYQAYALYYEKLKKFDDAEKMYHLGVQKLAEPIDELQR 140
            +D   + + +EE  I    SL Y+ YA++ E    +  A  +Y  G+ + AEP D L+ 
Sbjct: 71  REDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDRLKE 130

Query: 141 SYEQFLRRMKK 151
           ++  FL+R+ K
Sbjct: 131 AHSLFLQRISK 141

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7015406.17.9e-301100.00Mitotic spindle checkpoint protein BUBR1 [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_022929208.16.9e-29798.84uncharacterized protein LOC111435869 [Cucurbita moschata][more]
KAG6577315.17.9e-29398.26Mitotic spindle checkpoint protein BUBR1, partial [Cucurbita argyrosperma subsp.... [more]
XP_023552017.16.7e-29296.90uncharacterized protein LOC111809814 [Cucurbita pepo subsp. pepo][more]
XP_022984556.15.9e-28896.71uncharacterized protein LOC111482816 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q54CV51.3e-1932.73Probable inactive serine/threonine-protein kinase bub1 OS=Dictyostelium discoide... [more]
O597672.4e-1836.09Mitotic spindle checkpoint component mad3 OS=Schizosaccharomyces pombe (strain 9... [more]
O228067.1e-1829.90Mitotic spindle checkpoint protein BUBR1 OS=Arabidopsis thaliana OX=3702 GN=BUBR... [more]
F4IVI01.8e-1332.82Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana ... [more]
Q9Z1S03.1e-1328.17Mitotic checkpoint serine/threonine-protein kinase BUB1 beta OS=Mus musculus OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1ERG03.4e-29798.84uncharacterized protein LOC111435869 OS=Cucurbita moschata OX=3662 GN=LOC1114358... [more]
A0A6J1J5L02.8e-28896.71uncharacterized protein LOC111482816 OS=Cucurbita maxima OX=3661 GN=LOC111482816... [more]
A0A1S3CCD91.3e-24080.71uncharacterized protein LOC103499203 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3D6J01.3e-24080.71Mad3_BUB1_I domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A5A7UEU22.9e-24080.52Mad3_BUB1_I domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
Match NameE-valueIdentityDescription
AT5G05510.14.1e-13051.82Mad3/BUB1 homology region 1 [more]
AT2G33560.22.3e-1929.76BUB1-related (BUB1: budding uninhibited by benzymidazol 1) [more]
AT2G33560.15.0e-1929.90BUB1-related (BUB1: budding uninhibited by benzymidazol 1) [more]
AT2G20635.11.3e-1432.82ATP binding;protein kinases;protein serine/threonine kinases [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 134..154
NoneNo IPR availableGENE3D1.25.40.430coord: 8..165
e-value: 2.5E-52
score: 178.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 471..491
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 152..207
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..200
NoneNo IPR availablePANTHERPTHR14030:SF2OS11G0128700 PROTEINcoord: 4..516
IPR013212Mad3/Bub1 homology region 1SMARTSM00777mad3_bub1_icoord: 3..127
e-value: 1.2E-43
score: 161.0
IPR013212Mad3/Bub1 homology region 1PFAMPF08311Mad3_BUB1_Icoord: 11..127
e-value: 1.1E-34
score: 119.1
IPR013212Mad3/Bub1 homology region 1PROSITEPS51489BUB1_Ncoord: 10..166
score: 48.615459
IPR015661Mitotic spindle checkpoint protein Bub1/Mad3PANTHERPTHR14030MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1coord: 4..516

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05840-RACarg05840-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051754 meiotic sister chromatid cohesion, centromeric
biological_process GO:0007094 mitotic spindle assembly checkpoint signaling
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0043229 intracellular organelle
molecular_function GO:0004672 protein kinase activity