Homology
BLAST of Carg26781 vs. NCBI nr
Match:
KAG7016799.1 (CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 620/620 (100.00%), Postives = 620/620 (100.00%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK
Sbjct: 1 MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
Query: 61 FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120
FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG
Sbjct: 61 FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120
Query: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN
Sbjct: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
Query: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG
Sbjct: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
Query: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300
IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL
Sbjct: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300
Query: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI
Sbjct: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
Query: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR
Sbjct: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
Query: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT
Sbjct: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
Query: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS
Sbjct: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
Query: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 600
VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS
Sbjct: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 600
Query: 601 FFGYTRFLHGVTIRYSNTRH 621
FFGYTRFLHGVTIRYSNTRH
Sbjct: 601 FFGYTRFLHGVTIRYSNTRH 620
BLAST of Carg26781 vs. NCBI nr
Match:
XP_022922250.1 (CDPK-related kinase 4 isoform X2 [Cucurbita moschata])
HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 562/620 (90.65%), Postives = 564/620 (90.97%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPART RRK
Sbjct: 1 MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTTRRK 60
Query: 61 FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120
FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGAKFELG
Sbjct: 61 FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELG 120
Query: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN
Sbjct: 121 REVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
Query: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG
Sbjct: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
Query: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300
IVHRDLKPE NFLFMKKDENTGLKLIDFGLSDFVKP
Sbjct: 241 IVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP----------------- 300
Query: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI
Sbjct: 301 ----------------------------DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
Query: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR
Sbjct: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
Query: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDELFYLRT
Sbjct: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRT 480
Query: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS
Sbjct: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
Query: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 600
VYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS
Sbjct: 541 VYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 567
Query: 601 FFGYTRFLHGVTIRYSNTRH 621
FFGYTRFLHGVTIRYSNTRH
Sbjct: 601 FFGYTRFLHGVTIRYSNTRH 567
BLAST of Carg26781 vs. NCBI nr
Match:
XP_023549645.1 (CDPK-related kinase 6-like isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 554/625 (88.64%), Postives = 560/625 (89.60%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60
MGHHCSKSVSD+SNHAVISGGS P+ EDTRHSNSVSATASPFSSPLPSGIAPSPAR
Sbjct: 1 MGHHCSKSVSDVSNHAVISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSGIAPSPAR 60
Query: 61 TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGA 120
TPRRKFRWPLPPPSPAKPIMAILRRKSRKASI EENQI EEGLGEVQLDKSFGYS NFGA
Sbjct: 61 TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIQEENQIREEGLGEVQLDKSFGYSTNFGA 120
Query: 121 KFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180
KFELG+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTST+ IEDVRREVKILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTIAIEDVRREVKILKAL 180
Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
Query: 241 CHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWA 300
CHLQGIVHRDLKPE NFLFMKKDENTGLKLIDFGLSDFVKP
Sbjct: 241 CHLQGIVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP------------ 300
Query: 301 VLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEA 360
DERLNDVVGSAYYVAPEVLYRSYSFEA
Sbjct: 301 ---------------------------------DERLNDVVGSAYYVAPEVLYRSYSFEA 360
Query: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK
Sbjct: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
Query: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDEL 480
DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDEL
Sbjct: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDEL 480
Query: 481 FYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540
FYLRTQFKLL+PQNGFVTIDNYK ALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC
Sbjct: 481 FYLRTQFKLLEPQNGFVTIDNYKTALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540
Query: 541 AAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 600
AAAISVYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQEMNLGPIAHSFLQNWTRSS
Sbjct: 541 AAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 572
Query: 601 DGKLSFFGYTRFLHGVTIRYSNTRH 621
DGKLSFFGYTRFLHGVTIRYSNTRH
Sbjct: 601 DGKLSFFGYTRFLHGVTIRYSNTRH 572
BLAST of Carg26781 vs. NCBI nr
Match:
XP_022974330.1 (CDPK-related kinase 6-like isoform X2 [Cucurbita maxima])
HSP 1 Score: 1069.3 bits (2764), Expect = 1.2e-308
Identity = 551/625 (88.16%), Postives = 560/625 (89.60%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60
MGHHCSKSVSD+S+HA ISGGS P+ EDTRHSNSVSATASPFSSPLPS IAPSPAR
Sbjct: 1 MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
Query: 61 TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGA 120
T RRKFRWPLPPPSPAKPIMAILR+KSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGA
Sbjct: 61 T-RRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120
Query: 121 KFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180
KFELG+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK+KMTSTVGIEDVRRE KILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKAL 180
Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
Query: 241 CHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWA 300
CHLQGIVHRDLKPE NFLFMKKDENTGLKLIDFGLSDFVKP
Sbjct: 241 CHLQGIVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP------------ 300
Query: 301 VLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEA 360
DERLNDVVGSAYYVAPEVLYRSYSFEA
Sbjct: 301 ---------------------------------DERLNDVVGSAYYVAPEVLYRSYSFEA 360
Query: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK
Sbjct: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
Query: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDEL 480
DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDEL
Sbjct: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDEL 480
Query: 481 FYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540
FYLRTQFKLL+PQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC
Sbjct: 481 FYLRTQFKLLEPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540
Query: 541 AAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 600
AAAISVYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQEMNLGPIAHSFLQNWTRSS
Sbjct: 541 AAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 571
Query: 601 DGKLSFFGYTRFLHGVTIRYSNTRH 621
DGKLSFFGYT+FLHGVTIRYSNTRH
Sbjct: 601 DGKLSFFGYTKFLHGVTIRYSNTRH 571
BLAST of Carg26781 vs. NCBI nr
Match:
KAG6579299.1 (CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1016.9 bits (2628), Expect = 7.3e-293
Identity = 523/579 (90.33%), Postives = 523/579 (90.33%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK
Sbjct: 1 MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
Query: 61 FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120
FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGAKFELG
Sbjct: 61 FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELG 120
Query: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN
Sbjct: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
Query: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG
Sbjct: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
Query: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300
IVHRDLKPE NFLFMKKDENTGLKLIDFGLSDFVKP
Sbjct: 241 IVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP----------------- 300
Query: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI
Sbjct: 301 ----------------------------DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
Query: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR
Sbjct: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
Query: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT
Sbjct: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
Query: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS
Sbjct: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 526
Query: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMN 580
VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQ N
Sbjct: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQVRN 526
BLAST of Carg26781 vs. ExPASy Swiss-Prot
Match:
Q9FIM9 (CDPK-related kinase 4 OS=Arabidopsis thaliana OX=3702 GN=CRK4 PE=1 SV=1)
HSP 1 Score: 708.0 bits (1826), Expect = 9.5e-203
Identity = 369/646 (57.12%), Postives = 467/646 (72.29%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISG----GSHPTEDTRHSNSV------------SATASPFSSP 60
MGH S+++S + + + +G + P+++ RH++ S SPF SP
Sbjct: 1 MGHCYSRNISAVEDDEIPTGNGEVSNQPSQNHRHASIPQSPVASGTPEVNSYNISPFQSP 60
Query: 61 LPSGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEENQI----- 120
LP+G+APSPARTP RKF+WP PPPSPAKPIMA LRR+ R IPE+++
Sbjct: 61 LPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGAPPQPRDEPIPEDSEDVVDHG 120
Query: 121 GEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK 180
G+ G GE +LDK+FG+ NF K+ELGKEVGRGHFGHT WAK KKG +K + VAVKIISK
Sbjct: 121 GDSGGGE-RLDKNFGFGKNFEGKYELGKEVGRGHFGHTCWAKAKKGKMKNQTVAVKIISK 180
Query: 181 SKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSR 240
+KMTST+ IEDVRREVK+LKALSGH+++V F+ +EDA+N+++VMELCEGGELL+RI++R
Sbjct: 181 AKMTSTLSIEDVRREVKLLKALSGHRHMVKFYDVYEDADNVFVVMELCEGGELLDRILAR 240
Query: 241 GGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLI 300
GGRY E ++K I+VQIL AF HLQG+VHRDLKPE NFLF ++E+ LK+I
Sbjct: 241 GGRYPEVDAKRILVQILSATAFFHLQGVVHRDLKPE--------NFLFTSRNEDAILKVI 300
Query: 301 DFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLN 360
DFGLSDF++ D+RLN
Sbjct: 301 DFGLSDFIR---------------------------------------------YDQRLN 360
Query: 361 DVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNF 420
DVVGSAYYVAPEVL+RSYS EADMWSIGVI+YILLCGSRPF+ RTES IFR VLRA+PNF
Sbjct: 361 DVVGSAYYVAPEVLHRSYSTEADMWSIGVISYILLCGSRPFYGRTESAIFRCVLRANPNF 420
Query: 421 DDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL 480
+D PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+ + LD +YK VK+Y+
Sbjct: 421 EDMPWPSISPTAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSVYKLVKSYI 480
Query: 481 CATPFKRAALKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESN 540
A+PF+R+ALKAL+KA+ ++EL +L+ QF LLDP++G ++++ + AL R TDAM ES
Sbjct: 481 RASPFRRSALKALSKAIPDEELVFLKAQFMLLDPKDGGLSLNCFTMALTRYATDAMMESR 540
Query: 541 VADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHE 600
+ DIL M+PLA K++ FEEFCAAA+SVYQLEA+ WE IAT AFE+FE +GNR+ISV E
Sbjct: 541 LPDILNTMQPLAQKKLDFEEFCAAAVSVYQLEALEEWEQIATSAFEHFEHEGNRIISVQE 592
Query: 601 LVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
L EM++GP A+ L++W RSSDGKLSF GY +FLHGVT+R S++R
Sbjct: 601 LAGEMSVGPSAYPLLKDWIRSSDGKLSFLGYAKFLHGVTVRSSSSR 592
BLAST of Carg26781 vs. ExPASy Swiss-Prot
Match:
Q9SG12 (CDPK-related kinase 6 OS=Arabidopsis thaliana OX=3702 GN=CRK6 PE=1 SV=1)
HSP 1 Score: 703.7 bits (1815), Expect = 1.8e-201
Identity = 369/645 (57.21%), Postives = 461/645 (71.47%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGS----HPTEDTRHSNSVSAT--------------ASPFS 60
MGH S+++S + + I + H + H S S++ SPF
Sbjct: 1 MGHCYSRNISTVDDDDEIPSATAQLPHRSHQNHHQTSSSSSIPQSPATSEVNPYNISPFQ 60
Query: 61 SPLPSGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEENQIGEE 120
SPLP+G+APSPARTP RKF+WP PPPSPAKPIMA LRR+ R IPE+++ G
Sbjct: 61 SPLPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGTAPHPRDGPIPEDSEAGGS 120
Query: 121 --GLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKS 180
G+GE +LDK+FG++ NF K+ELG+EVGRGHFGHT WAK KKG +KG+ VAVKIISKS
Sbjct: 121 GGGIGE-RLDKNFGFAKNFEGKYELGREVGRGHFGHTCWAKAKKGKIKGQTVAVKIISKS 180
Query: 181 KMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRG 240
KMTS + IEDVRREVK+LKALSGH ++V F+ FED++N+++VMELCEGGELL+ I++RG
Sbjct: 181 KMTSALSIEDVRREVKLLKALSGHSHMVKFYDVFEDSDNVFVVMELCEGGELLDSILARG 240
Query: 241 GRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLID 300
GRY E E+K I+VQIL AF HLQG+VHRDLKPE NFLF K+E+ LK+ID
Sbjct: 241 GRYPEAEAKRILVQILSATAFFHLQGVVHRDLKPE--------NFLFTSKNEDAVLKVID 300
Query: 301 FGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLND 360
FGLSD+ + D+RLND
Sbjct: 301 FGLSDYAR---------------------------------------------FDQRLND 360
Query: 361 VVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFD 420
VVGSAYYVAPEVL+RSYS EAD+WSIGVI+YILLCGSRPF+ RTES IFR VLRA+PNFD
Sbjct: 361 VVGSAYYVAPEVLHRSYSTEADIWSIGVISYILLCGSRPFYGRTESAIFRCVLRANPNFD 420
Query: 421 DSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLC 480
D PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+ + LD IYK VK+Y+
Sbjct: 421 DLPWPSISPIAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSIYKLVKSYIR 480
Query: 481 ATPFKRAALKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNV 540
A+PF+RAALK+L+KA+ E+EL +L+ QF LL+P++G + + N+ AL R TDAM ES +
Sbjct: 481 ASPFRRAALKSLSKAIPEEELVFLKAQFMLLEPEDGGLHLHNFTTALTRYATDAMIESRL 540
Query: 541 ADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHEL 600
DIL MM+PLA+K++ FEEFCAA++SVYQLEA+ WE IAT AFE+FE +G+R ISV EL
Sbjct: 541 PDILNMMQPLAHKKLDFEEFCAASVSVYQLEALEEWEQIATVAFEHFESEGSRAISVQEL 591
Query: 601 VQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
+EM+LGP A+ L++W RS DGKL+F GY +FLHGVT+R S++R
Sbjct: 601 AEEMSLGPNAYPLLKDWIRSLDGKLNFLGYAKFLHGVTVRSSSSR 591
BLAST of Carg26781 vs. ExPASy Swiss-Prot
Match:
Q9ZUZ2 (CDPK-related kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRK3 PE=1 SV=1)
HSP 1 Score: 700.7 bits (1807), Expect = 1.5e-200
Identity = 367/600 (61.17%), Postives = 445/600 (74.17%), Query Frame = 0
Query: 31 SNSVSATASPF----SSPLPSGIAPSPAR--TPRRKFRWPLPPPSPAKPIMAILRR---- 90
S++ S SPF +SPLPSG++PSPAR TPRR FR P PPPSPAK I A L +
Sbjct: 51 SSNPSPWPSPFPHGSASPLPSGVSPSPARTSTPRRFFRRPFPPPSPAKHIKASLIKRLGV 110
Query: 91 KSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGD 150
K ++ IPEE G E E LDKSFGY NFGAK+ELGKEVGRGHFGHT +GKKGD
Sbjct: 111 KPKEGPIPEER--GTE--PEQSLDKSFGYGKNFGAKYELGKEVGRGHFGHTCSGRGKKGD 170
Query: 151 LKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMEL 210
+K P+AVKIISK+KMT+ + IEDVRREVK+LK+LSGHK L+ ++ A EDANN+YIVMEL
Sbjct: 171 IKDHPIAVKIISKAKMTTAIAIEDVRREVKLLKSLSGHKYLIKYYDACEDANNVYIVMEL 230
Query: 211 CEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFL 270
C+GGELL+RI++RGG+Y E ++K I+VQIL VV+FCHLQG+VHRDLKPE NFL
Sbjct: 231 CDGGELLDRILARGGKYPEDDAKAIVVQILTVVSFCHLQGVVHRDLKPE--------NFL 290
Query: 271 FMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVF 330
F E++ LKLIDFGLSDF++P
Sbjct: 291 FTSSREDSDLKLIDFGLSDFIRP------------------------------------- 350
Query: 331 TTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTES 390
DERLND+VGSAYYVAPEVL+RSYS EAD+WSIGVI YILLCGSRPFWARTES
Sbjct: 351 --------DERLNDIVGSAYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTES 410
Query: 391 GIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVP 450
GIFR+VLR +PN+DD PWP+ S E KDFVKRLLNKD+RKRM+A QALTHPWLRD+ +P
Sbjct: 411 GIFRTVLRTEPNYDDVPWPSCSSEGKDFVKRLLNKDYRKRMSAVQALTHPWLRDDSRVIP 470
Query: 451 LDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKA 510
LD IYK VKAYL ATP +RAALKALAKALTE+EL YLR QF LL P ++G V+++N+K
Sbjct: 471 LDILIYKLVKAYLHATPLRRAALKALAKALTENELVYLRAQFMLLGPNKDGSVSLENFKT 530
Query: 511 ALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFE 570
AL++N TDAMRES V +IL ME LA ++M FEEFCAAAIS++QLEAV WE IAT F+
Sbjct: 531 ALMQNATDAMRESRVPEILHTMESLAYRKMYFEEFCAAAISIHQLEAVDAWEEIATAGFQ 590
Query: 571 YFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
+FE +GNRVI++ EL +E+N+G A+ L++W RSSDGKLS+ G+T+FLHGVT+R ++ R
Sbjct: 591 HFETEGNRVITIEELARELNVGASAYGHLRDWVRSSDGKLSYLGFTKFLHGVTLRAAHAR 593
BLAST of Carg26781 vs. ExPASy Swiss-Prot
Match:
Q9SCS2 (CDPK-related kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CRK5 PE=2 SV=1)
HSP 1 Score: 624.4 bits (1609), Expect = 1.4e-177
Identity = 329/596 (55.20%), Postives = 421/596 (70.64%), Query Frame = 0
Query: 40 PFSSPLPSGI-------APSPA-----RTPRRKFRWPLPPPSPAKPIMAILRR-----KS 99
PF SP P+ A SPA TP+R F+ P PPPSPAK I A+L R K
Sbjct: 57 PFYSPSPAHYFFSKKTPARSPATNSTNSTPKRFFKRPFPPPSPAKHIRAVLARRHGSVKP 116
Query: 100 RKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLK 159
++IPE ++ G+G LDKSFG+S +F +K+ELG EVGRGHFG+T AK KKGD K
Sbjct: 117 NSSAIPEGSEAEGGGVG---LDKSFGFSKSFASKYELGDEVGRGHFGYTCAAKFKKGDNK 176
Query: 160 GKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCE 219
G+ VAVK+I K+KMT+ + IEDVRREVKIL+ALSGH NL HF+ A+ED +N+YIVMELCE
Sbjct: 177 GQQVAVKVIPKAKMTTAIAIEDVRREVKILRALSGHNNLPHFYDAYEDHDNVYIVMELCE 236
Query: 220 GGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFM 279
GGELL+RI+SRGG+Y E+++KT+++QIL VVAFCHLQG+VHRDLKPE NFLF
Sbjct: 237 GGELLDRILSRGGKYTEEDAKTVMIQILNVVAFCHLQGVVHRDLKPE--------NFLFT 296
Query: 280 KKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTT 339
K++ + LK IDFGLSD+V+P
Sbjct: 297 SKEDTSQLKAIDFGLSDYVRP--------------------------------------- 356
Query: 340 PISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGI 399
DERLND+VGSAYYVAPEVL+RSYS EAD+WS+GVI YILLCGSRPFWARTESGI
Sbjct: 357 ------DERLNDIVGSAYYVAPEVLHRSYSTEADIWSVGVIVYILLCGSRPFWARTESGI 416
Query: 400 FRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRD-EDLAVPL 459
FR+VL+ADP+FDD PWP +S EA+DFVKRLLNKD RKR+TAAQAL+HPW++D D VP+
Sbjct: 417 FRAVLKADPSFDDPPWPLLSSEARDFVKRLLNKDPRKRLTAAQALSHPWIKDSNDAKVPM 476
Query: 460 DNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKAA 519
D ++K ++AYL ++ ++AAL+AL+K LT DELFYLR QF LL+P +NG ++++N K+A
Sbjct: 477 DILVFKLMRAYLRSSSLRKAALRALSKTLTVDELFYLREQFALLEPSKNGTISLENIKSA 536
Query: 520 LVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEY 579
L++ TDAM++S + + L + L +RM FEEFCAAA+SV+QLEA+ WE A A+E
Sbjct: 537 LMKMATDAMKDSRIPEFLGQLSALQYRRMDFEEFCAAALSVHQLEALDRWEQHARCAYEL 596
Query: 580 FEQDGNRVISVHELVQEMNLGPI--AHSFLQNWTRSSDGKLSFFGYTRFLHGVTIR 615
FE++GNR I + EL E+ LGP H+ L +W R +DGKLSF G+ + LHGV+ R
Sbjct: 597 FEKEGNRPIMIDELASELGLGPSVPVHAVLHDWLRHTDGKLSFLGFVKLLHGVSSR 596
BLAST of Carg26781 vs. ExPASy Swiss-Prot
Match:
P53681 (CDPK-related protein kinase OS=Daucus carota OX=4039 GN=CRK PE=2 SV=1)
HSP 1 Score: 620.2 bits (1598), Expect = 2.6e-176
Identity = 340/639 (53.21%), Postives = 428/639 (66.98%), Query Frame = 0
Query: 3 HHCSKSVSDIS-----NHAVISGGSHPTEDTRHSNSVSATASPFSSPLP----------- 62
HH S V+D S N + P +D + PF SP P
Sbjct: 17 HHTSIPVNDTSLPPQDNSIPPKDIAIPAQDNNKPPGKKSPFLPFYSPSPAHFLFSKKSPA 76
Query: 63 --SGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRR-----KSRKASIPEENQIGEEGLG 122
S A S TP+R F P PPPSPAK I A R K +A+IPE N++ G
Sbjct: 77 VGSPAAGSSNSTPKRLF--PFPPPSPAKHIKAAWARRHGSVKPNEAAIPENNEVD----G 136
Query: 123 EVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTST 182
LDKSFG+S FG+KFE+G+EVGRGHFG+T AK KKG+ KG+ VAVK+I K+KMT+
Sbjct: 137 GAGLDKSFGFSKKFGSKFEVGEEVGRGHFGYTCRAKFKKGEFKGQDVAVKVIPKAKMTTA 196
Query: 183 VGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQE 242
+ IEDVRREVKIL+AL+GH NLV F+ AFED N+Y+VMELCEGGELL+RI+SRGG+Y E
Sbjct: 197 IAIEDVRREVKILRALTGHNNLVQFYDAFEDHTNVYVVMELCEGGELLDRILSRGGKYTE 256
Query: 243 QESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSD 302
++K +++QIL VVAFCHLQG+VHRDLKPE NFLF KDE++ LK IDFGLSD
Sbjct: 257 DDAKAVMIQILNVVAFCHLQGVVHRDLKPE--------NFLFKSKDEDSQLKAIDFGLSD 316
Query: 303 FVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSA 362
+VKP DERLND+VGSA
Sbjct: 317 YVKP---------------------------------------------DERLNDIVGSA 376
Query: 363 YYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWP 422
YYVAPEVL+RSYS EAD+WSIGVI+YILLCGSRPFWARTESGIFR+VL+A+ +FD+ PWP
Sbjct: 377 YYVAPEVLHRSYSTEADVWSIGVISYILLCGSRPFWARTESGIFRAVLKANLSFDEPPWP 436
Query: 423 TISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRD-EDLAVPLDNFIYKSVKAYLCATPF 482
++S EAKDFVKRLLNKD RKRMTAAQAL H W+++ D+ PLD ++K +K Y+ ++P
Sbjct: 437 SVSSEAKDFVKRLLNKDPRKRMTAAQALCHSWIKNSNDIKFPLDILVFKLMKVYMRSSPL 496
Query: 483 KRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKAALVRNVTDAMRESNVADI 542
++AAL+AL+K LT DELFYL+ QF LL+P +NG ++++N K AL+RN TDAM++S V D+
Sbjct: 497 RKAALRALSKTLTVDELFYLKEQFVLLEPTKNGTISLENIKQALMRNSTDAMKDSRVLDL 556
Query: 543 LKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQE 602
L + L +RM FEEFCAAA+SV+QLEA+ WE A A++ FE+DGNR I + EL E
Sbjct: 557 LVSLNALQYRRMDFEEFCAAALSVHQLEALDRWEQHARCAYDLFEKDGNRAIMIEELASE 596
Query: 603 MNLGPI--AHSFLQNWTRSSDGKLSFFGYTRFLHGVTIR 615
+ LGP H+ L +W R +DGKLSF GY + LHGV+ R
Sbjct: 617 LGLGPSIPVHAVLHDWIRHTDGKLSFLGYVKLLHGVSTR 596
BLAST of Carg26781 vs. ExPASy TrEMBL
Match:
A0A6J1E874 (CDPK-related kinase 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430287 PE=4 SV=1)
HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 562/620 (90.65%), Postives = 564/620 (90.97%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPART RRK
Sbjct: 1 MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTTRRK 60
Query: 61 FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120
FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGAKFELG
Sbjct: 61 FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELG 120
Query: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN
Sbjct: 121 REVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
Query: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG
Sbjct: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
Query: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300
IVHRDLKPE NFLFMKKDENTGLKLIDFGLSDFVKP
Sbjct: 241 IVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP----------------- 300
Query: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI
Sbjct: 301 ----------------------------DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
Query: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR
Sbjct: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
Query: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDELFYLRT
Sbjct: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRT 480
Query: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS
Sbjct: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
Query: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 600
VYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS
Sbjct: 541 VYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 567
Query: 601 FFGYTRFLHGVTIRYSNTRH 621
FFGYTRFLHGVTIRYSNTRH
Sbjct: 601 FFGYTRFLHGVTIRYSNTRH 567
BLAST of Carg26781 vs. ExPASy TrEMBL
Match:
A0A6J1IDP0 (CDPK-related kinase 6-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472964 PE=4 SV=1)
HSP 1 Score: 1069.3 bits (2764), Expect = 6.0e-309
Identity = 551/625 (88.16%), Postives = 560/625 (89.60%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60
MGHHCSKSVSD+S+HA ISGGS P+ EDTRHSNSVSATASPFSSPLPS IAPSPAR
Sbjct: 1 MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
Query: 61 TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGA 120
T RRKFRWPLPPPSPAKPIMAILR+KSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGA
Sbjct: 61 T-RRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120
Query: 121 KFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180
KFELG+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK+KMTSTVGIEDVRRE KILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKAL 180
Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
Query: 241 CHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWA 300
CHLQGIVHRDLKPE NFLFMKKDENTGLKLIDFGLSDFVKP
Sbjct: 241 CHLQGIVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP------------ 300
Query: 301 VLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEA 360
DERLNDVVGSAYYVAPEVLYRSYSFEA
Sbjct: 301 ---------------------------------DERLNDVVGSAYYVAPEVLYRSYSFEA 360
Query: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK
Sbjct: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
Query: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDEL 480
DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDEL
Sbjct: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDEL 480
Query: 481 FYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540
FYLRTQFKLL+PQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC
Sbjct: 481 FYLRTQFKLLEPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540
Query: 541 AAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 600
AAAISVYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQEMNLGPIAHSFLQNWTRSS
Sbjct: 541 AAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 571
Query: 601 DGKLSFFGYTRFLHGVTIRYSNTRH 621
DGKLSFFGYT+FLHGVTIRYSNTRH
Sbjct: 601 DGKLSFFGYTKFLHGVTIRYSNTRH 571
BLAST of Carg26781 vs. ExPASy TrEMBL
Match:
A0A6J1E2V0 (CDPK-related kinase 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430287 PE=4 SV=1)
HSP 1 Score: 1006.5 bits (2601), Expect = 4.8e-290
Identity = 519/579 (89.64%), Postives = 521/579 (89.98%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPART RRK
Sbjct: 1 MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTTRRK 60
Query: 61 FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120
FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGAKFELG
Sbjct: 61 FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELG 120
Query: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN
Sbjct: 121 REVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
Query: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG
Sbjct: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
Query: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300
IVHRDLKPE NFLFMKKDENTGLKLIDFGLSDFVKP
Sbjct: 241 IVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP----------------- 300
Query: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI
Sbjct: 301 ----------------------------DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
Query: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR
Sbjct: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
Query: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDELFYLRT
Sbjct: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRT 480
Query: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS
Sbjct: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 526
Query: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMN 580
VYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQ N
Sbjct: 541 VYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQVRN 526
BLAST of Carg26781 vs. ExPASy TrEMBL
Match:
A0A6J1IHA7 (CDPK-related kinase 6-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472964 PE=4 SV=1)
HSP 1 Score: 978.8 bits (2529), Expect = 1.1e-281
Identity = 509/584 (87.16%), Postives = 517/584 (88.53%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60
MGHHCSKSVSD+S+HA ISGGS P+ EDTRHSNSVSATASPFSSPLPS IAPSPAR
Sbjct: 1 MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60
Query: 61 TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGA 120
T RRKFRWPLPPPSPAKPIMAILR+KSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGA
Sbjct: 61 T-RRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120
Query: 121 KFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180
KFELG+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK+KMTSTVGIEDVRRE KILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKAL 180
Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
Query: 241 CHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWA 300
CHLQGIVHRDLKPE NFLFMKKDENTGLKLIDFGLSDFVKP
Sbjct: 241 CHLQGIVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP------------ 300
Query: 301 VLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEA 360
DERLNDVVGSAYYVAPEVLYRSYSFEA
Sbjct: 301 ---------------------------------DERLNDVVGSAYYVAPEVLYRSYSFEA 360
Query: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK
Sbjct: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
Query: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDEL 480
DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDEL
Sbjct: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDEL 480
Query: 481 FYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540
FYLRTQFKLL+PQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC
Sbjct: 481 FYLRTQFKLLEPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 530
Query: 541 AAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMN 580
AAAISVYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQ N
Sbjct: 541 AAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQVRN 530
BLAST of Carg26781 vs. ExPASy TrEMBL
Match:
A0A6J1K6B2 (CDPK-related kinase 6-like OS=Cucurbita maxima OX=3661 GN=LOC111491067 PE=4 SV=1)
HSP 1 Score: 974.5 bits (2518), Expect = 2.0e-280
Identity = 505/638 (79.15%), Postives = 530/638 (83.07%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGSHP------------------TEDTRHSNSVSATASPFS 60
MGH CSKSVSD+++ ISGGSH E+ R S SVSAT SPFS
Sbjct: 1 MGHLCSKSVSDVNHDTGISGGSHTRSSHSGASPIAYGDVYSRPEEFRQSYSVSATGSPFS 60
Query: 61 SPLPSGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQ 120
SPLP+GIAPSPARTP RKFRWPLPPPSPAKPIMAILRRKSRKASIPEEN+IGEEG+GE+Q
Sbjct: 61 SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENRIGEEGVGEIQ 120
Query: 121 LDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGI 180
LDKSFGYS NFGAKFELGKE+GRGHFGHTFWA+GKKGDLKGKPVAVKIISKSKMTS VGI
Sbjct: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWARGKKGDLKGKPVAVKIISKSKMTSAVGI 180
Query: 181 EDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQES 240
EDVRREVKILKALSGHKNLV FH AFEDANNIY+VMELCEGGELLERIVSRGGRY EQES
Sbjct: 181 EDVRREVKILKALSGHKNLVQFHDAFEDANNIYMVMELCEGGELLERIVSRGGRYPEQES 240
Query: 241 KTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVK 300
KTI+VQIL VVAF HLQG+VHRDLKPE NFLF+KKDENTGLK+IDFGLSDFVK
Sbjct: 241 KTILVQILNVVAFFHLQGVVHRDLKPE--------NFLFVKKDENTGLKVIDFGLSDFVK 300
Query: 301 PERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYV 360
P DE LNDVVGSAYYV
Sbjct: 301 P---------------------------------------------DEHLNDVVGSAYYV 360
Query: 361 APEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTIS 420
APEVLYRSYS EAD+WSIGVIAYILLCG RPFWARTESGIFRSVLRADPNFDDSPWPTIS
Sbjct: 361 APEVLYRSYSHEADVWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDDSPWPTIS 420
Query: 421 PEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAA 480
EAKDFVKRLLNKDHRKRMTA QALTHPWLRDE++AVPLD+FIYKSVKAYL TPFKRAA
Sbjct: 421 AEAKDFVKRLLNKDHRKRMTAVQALTHPWLRDENVAVPLDSFIYKSVKAYLRVTPFKRAA 480
Query: 481 LKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMME 540
LKALAKALTEDELFYLR QFKLL+PQNGFVT+DN+K ALVRN TDAMRESNV DILKM E
Sbjct: 481 LKALAKALTEDELFYLRAQFKLLEPQNGFVTLDNFKEALVRNATDAMRESNVLDILKMTE 540
Query: 541 PLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGP 600
+A +RM FEEFCAAAISVYQLEAVAGWE IATRAFEYFEQDGNRVISVHELVQEMNL P
Sbjct: 541 SIAYERMDFEEFCAAAISVYQLEAVAGWEGIATRAFEYFEQDGNRVISVHELVQEMNLSP 585
Query: 601 IAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTRH 621
AHSFLQNWTRSSDGKLSFFGYTRFLHGVT+R SNTRH
Sbjct: 601 AAHSFLQNWTRSSDGKLSFFGYTRFLHGVTVRNSNTRH 585
BLAST of Carg26781 vs. TAIR 10
Match:
AT5G24430.1 (Calcium-dependent protein kinase (CDPK) family protein )
HSP 1 Score: 708.0 bits (1826), Expect = 6.7e-204
Identity = 369/646 (57.12%), Postives = 467/646 (72.29%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISG----GSHPTEDTRHSNSV------------SATASPFSSP 60
MGH S+++S + + + +G + P+++ RH++ S SPF SP
Sbjct: 1 MGHCYSRNISAVEDDEIPTGNGEVSNQPSQNHRHASIPQSPVASGTPEVNSYNISPFQSP 60
Query: 61 LPSGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEENQI----- 120
LP+G+APSPARTP RKF+WP PPPSPAKPIMA LRR+ R IPE+++
Sbjct: 61 LPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGAPPQPRDEPIPEDSEDVVDHG 120
Query: 121 GEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK 180
G+ G GE +LDK+FG+ NF K+ELGKEVGRGHFGHT WAK KKG +K + VAVKIISK
Sbjct: 121 GDSGGGE-RLDKNFGFGKNFEGKYELGKEVGRGHFGHTCWAKAKKGKMKNQTVAVKIISK 180
Query: 181 SKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSR 240
+KMTST+ IEDVRREVK+LKALSGH+++V F+ +EDA+N+++VMELCEGGELL+RI++R
Sbjct: 181 AKMTSTLSIEDVRREVKLLKALSGHRHMVKFYDVYEDADNVFVVMELCEGGELLDRILAR 240
Query: 241 GGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLI 300
GGRY E ++K I+VQIL AF HLQG+VHRDLKPE NFLF ++E+ LK+I
Sbjct: 241 GGRYPEVDAKRILVQILSATAFFHLQGVVHRDLKPE--------NFLFTSRNEDAILKVI 300
Query: 301 DFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLN 360
DFGLSDF++ D+RLN
Sbjct: 301 DFGLSDFIR---------------------------------------------YDQRLN 360
Query: 361 DVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNF 420
DVVGSAYYVAPEVL+RSYS EADMWSIGVI+YILLCGSRPF+ RTES IFR VLRA+PNF
Sbjct: 361 DVVGSAYYVAPEVLHRSYSTEADMWSIGVISYILLCGSRPFYGRTESAIFRCVLRANPNF 420
Query: 421 DDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL 480
+D PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+ + LD +YK VK+Y+
Sbjct: 421 EDMPWPSISPTAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSVYKLVKSYI 480
Query: 481 CATPFKRAALKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESN 540
A+PF+R+ALKAL+KA+ ++EL +L+ QF LLDP++G ++++ + AL R TDAM ES
Sbjct: 481 RASPFRRSALKALSKAIPDEELVFLKAQFMLLDPKDGGLSLNCFTMALTRYATDAMMESR 540
Query: 541 VADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHE 600
+ DIL M+PLA K++ FEEFCAAA+SVYQLEA+ WE IAT AFE+FE +GNR+ISV E
Sbjct: 541 LPDILNTMQPLAQKKLDFEEFCAAAVSVYQLEALEEWEQIATSAFEHFEHEGNRIISVQE 592
Query: 601 LVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
L EM++GP A+ L++W RSSDGKLSF GY +FLHGVT+R S++R
Sbjct: 601 LAGEMSVGPSAYPLLKDWIRSSDGKLSFLGYAKFLHGVTVRSSSSR 592
BLAST of Carg26781 vs. TAIR 10
Match:
AT3G49370.1 (Calcium-dependent protein kinase (CDPK) family protein )
HSP 1 Score: 703.7 bits (1815), Expect = 1.3e-202
Identity = 369/645 (57.21%), Postives = 461/645 (71.47%), Query Frame = 0
Query: 1 MGHHCSKSVSDISNHAVISGGS----HPTEDTRHSNSVSAT--------------ASPFS 60
MGH S+++S + + I + H + H S S++ SPF
Sbjct: 1 MGHCYSRNISTVDDDDEIPSATAQLPHRSHQNHHQTSSSSSIPQSPATSEVNPYNISPFQ 60
Query: 61 SPLPSGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEENQIGEE 120
SPLP+G+APSPARTP RKF+WP PPPSPAKPIMA LRR+ R IPE+++ G
Sbjct: 61 SPLPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGTAPHPRDGPIPEDSEAGGS 120
Query: 121 --GLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKS 180
G+GE +LDK+FG++ NF K+ELG+EVGRGHFGHT WAK KKG +KG+ VAVKIISKS
Sbjct: 121 GGGIGE-RLDKNFGFAKNFEGKYELGREVGRGHFGHTCWAKAKKGKIKGQTVAVKIISKS 180
Query: 181 KMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRG 240
KMTS + IEDVRREVK+LKALSGH ++V F+ FED++N+++VMELCEGGELL+ I++RG
Sbjct: 181 KMTSALSIEDVRREVKLLKALSGHSHMVKFYDVFEDSDNVFVVMELCEGGELLDSILARG 240
Query: 241 GRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLID 300
GRY E E+K I+VQIL AF HLQG+VHRDLKPE NFLF K+E+ LK+ID
Sbjct: 241 GRYPEAEAKRILVQILSATAFFHLQGVVHRDLKPE--------NFLFTSKNEDAVLKVID 300
Query: 301 FGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLND 360
FGLSD+ + D+RLND
Sbjct: 301 FGLSDYAR---------------------------------------------FDQRLND 360
Query: 361 VVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFD 420
VVGSAYYVAPEVL+RSYS EAD+WSIGVI+YILLCGSRPF+ RTES IFR VLRA+PNFD
Sbjct: 361 VVGSAYYVAPEVLHRSYSTEADIWSIGVISYILLCGSRPFYGRTESAIFRCVLRANPNFD 420
Query: 421 DSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLC 480
D PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+ + LD IYK VK+Y+
Sbjct: 421 DLPWPSISPIAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSIYKLVKSYIR 480
Query: 481 ATPFKRAALKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNV 540
A+PF+RAALK+L+KA+ E+EL +L+ QF LL+P++G + + N+ AL R TDAM ES +
Sbjct: 481 ASPFRRAALKSLSKAIPEEELVFLKAQFMLLEPEDGGLHLHNFTTALTRYATDAMIESRL 540
Query: 541 ADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHEL 600
DIL MM+PLA+K++ FEEFCAA++SVYQLEA+ WE IAT AFE+FE +G+R ISV EL
Sbjct: 541 PDILNMMQPLAHKKLDFEEFCAASVSVYQLEALEEWEQIATVAFEHFESEGSRAISVQEL 591
Query: 601 VQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
+EM+LGP A+ L++W RS DGKL+F GY +FLHGVT+R S++R
Sbjct: 601 AEEMSLGPNAYPLLKDWIRSLDGKLNFLGYAKFLHGVTVRSSSSR 591
BLAST of Carg26781 vs. TAIR 10
Match:
AT2G46700.1 (CDPK-related kinase 3 )
HSP 1 Score: 700.7 bits (1807), Expect = 1.1e-201
Identity = 367/600 (61.17%), Postives = 445/600 (74.17%), Query Frame = 0
Query: 31 SNSVSATASPF----SSPLPSGIAPSPAR--TPRRKFRWPLPPPSPAKPIMAILRR---- 90
S++ S SPF +SPLPSG++PSPAR TPRR FR P PPPSPAK I A L +
Sbjct: 51 SSNPSPWPSPFPHGSASPLPSGVSPSPARTSTPRRFFRRPFPPPSPAKHIKASLIKRLGV 110
Query: 91 KSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGD 150
K ++ IPEE G E E LDKSFGY NFGAK+ELGKEVGRGHFGHT +GKKGD
Sbjct: 111 KPKEGPIPEER--GTE--PEQSLDKSFGYGKNFGAKYELGKEVGRGHFGHTCSGRGKKGD 170
Query: 151 LKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMEL 210
+K P+AVKIISK+KMT+ + IEDVRREVK+LK+LSGHK L+ ++ A EDANN+YIVMEL
Sbjct: 171 IKDHPIAVKIISKAKMTTAIAIEDVRREVKLLKSLSGHKYLIKYYDACEDANNVYIVMEL 230
Query: 211 CEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFL 270
C+GGELL+RI++RGG+Y E ++K I+VQIL VV+FCHLQG+VHRDLKPE NFL
Sbjct: 231 CDGGELLDRILARGGKYPEDDAKAIVVQILTVVSFCHLQGVVHRDLKPE--------NFL 290
Query: 271 FMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVF 330
F E++ LKLIDFGLSDF++P
Sbjct: 291 FTSSREDSDLKLIDFGLSDFIRP------------------------------------- 350
Query: 331 TTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTES 390
DERLND+VGSAYYVAPEVL+RSYS EAD+WSIGVI YILLCGSRPFWARTES
Sbjct: 351 --------DERLNDIVGSAYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTES 410
Query: 391 GIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVP 450
GIFR+VLR +PN+DD PWP+ S E KDFVKRLLNKD+RKRM+A QALTHPWLRD+ +P
Sbjct: 411 GIFRTVLRTEPNYDDVPWPSCSSEGKDFVKRLLNKDYRKRMSAVQALTHPWLRDDSRVIP 470
Query: 451 LDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKA 510
LD IYK VKAYL ATP +RAALKALAKALTE+EL YLR QF LL P ++G V+++N+K
Sbjct: 471 LDILIYKLVKAYLHATPLRRAALKALAKALTENELVYLRAQFMLLGPNKDGSVSLENFKT 530
Query: 511 ALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFE 570
AL++N TDAMRES V +IL ME LA ++M FEEFCAAAIS++QLEAV WE IAT F+
Sbjct: 531 ALMQNATDAMRESRVPEILHTMESLAYRKMYFEEFCAAAISIHQLEAVDAWEEIATAGFQ 590
Query: 571 YFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
+FE +GNRVI++ EL +E+N+G A+ L++W RSSDGKLS+ G+T+FLHGVT+R ++ R
Sbjct: 591 HFETEGNRVITIEELARELNVGASAYGHLRDWVRSSDGKLSYLGFTKFLHGVTLRAAHAR 593
BLAST of Carg26781 vs. TAIR 10
Match:
AT3G50530.2 (CDPK-related kinase )
HSP 1 Score: 636.0 bits (1639), Expect = 3.3e-182
Identity = 334/596 (56.04%), Postives = 434/596 (72.82%), Query Frame = 0
Query: 40 PFSSPLPSGI-------APSPA-----RTPRRKFRWPLPPPSPAKPIMAILRR-----KS 99
PF SP P+ A SPA TP+R F+ P PPPSPAK I A+L R K
Sbjct: 57 PFYSPSPAHYFFSKKTPARSPATNSTNSTPKRFFKRPFPPPSPAKHIRAVLARRHGSVKP 116
Query: 100 RKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLK 159
++IPE ++ G+G LDKSFG+S +F +K+ELG EVGRGHFG+T AK KKGD K
Sbjct: 117 NSSAIPEGSEAEGGGVG---LDKSFGFSKSFASKYELGDEVGRGHFGYTCAAKFKKGDNK 176
Query: 160 GKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCE 219
G+ VAVK+I K+KMT+ + IEDVRREVKIL+ALSGH NL HF+ A+ED +N+YIVMELCE
Sbjct: 177 GQQVAVKVIPKAKMTTAIAIEDVRREVKILRALSGHNNLPHFYDAYEDHDNVYIVMELCE 236
Query: 220 GGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFM 279
GGELL+RI+SRGG+Y E+++KT+++QIL VVAFCHLQG+VHRDLKPE NFLF
Sbjct: 237 GGELLDRILSRGGKYTEEDAKTVMIQILNVVAFCHLQGVVHRDLKPE--------NFLFT 296
Query: 280 KKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTT 339
K++ + LK IDFGLSD+V+P + + +A+ + ++ TS L L
Sbjct: 297 SKEDTSQLKAIDFGLSDYVRPGKAL----RLYAICKLRFQNLETSICLYAL--------- 356
Query: 340 PISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGI 399
+ DERLND+VGSAYYVAPEVL+RSYS EAD+WS+GVI YILLCGSRPFWARTESGI
Sbjct: 357 -TIAFADERLNDIVGSAYYVAPEVLHRSYSTEADIWSVGVIVYILLCGSRPFWARTESGI 416
Query: 400 FRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRD-EDLAVPL 459
FR+VL+ADP+FDD PWP +S EA+DFVKRLLNKD RKR+TAAQAL+HPW++D D VP+
Sbjct: 417 FRAVLKADPSFDDPPWPLLSSEARDFVKRLLNKDPRKRLTAAQALSHPWIKDSNDAKVPM 476
Query: 460 DNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKAA 519
D ++K ++AYL ++ ++AAL+AL+K LT DELFYLR QF LL+P +NG ++++N K+A
Sbjct: 477 DILVFKLMRAYLRSSSLRKAALRALSKTLTVDELFYLREQFALLEPSKNGTISLENIKSA 536
Query: 520 LVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEY 579
L++ TDAM++S + + L + L +RM FEEFCAAA+SV+QLEA+ WE A A+E
Sbjct: 537 LMKMATDAMKDSRIPEFLGQLSALQYRRMDFEEFCAAALSVHQLEALDRWEQHARCAYEL 596
Query: 580 FEQDGNRVISVHELVQEMNLGPI--AHSFLQNWTRSSDGKLSFFGYTRFLHGVTIR 615
FE++GNR I + EL E+ LGP H+ L +W R +DGKLSF G+ + LHGV+ R
Sbjct: 597 FEKEGNRPIMIDELASELGLGPSVPVHAVLHDWLRHTDGKLSFLGFVKLLHGVSSR 627
BLAST of Carg26781 vs. TAIR 10
Match:
AT3G50530.1 (CDPK-related kinase )
HSP 1 Score: 624.4 bits (1609), Expect = 9.8e-179
Identity = 329/596 (55.20%), Postives = 421/596 (70.64%), Query Frame = 0
Query: 40 PFSSPLPSGI-------APSPA-----RTPRRKFRWPLPPPSPAKPIMAILRR-----KS 99
PF SP P+ A SPA TP+R F+ P PPPSPAK I A+L R K
Sbjct: 57 PFYSPSPAHYFFSKKTPARSPATNSTNSTPKRFFKRPFPPPSPAKHIRAVLARRHGSVKP 116
Query: 100 RKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLK 159
++IPE ++ G+G LDKSFG+S +F +K+ELG EVGRGHFG+T AK KKGD K
Sbjct: 117 NSSAIPEGSEAEGGGVG---LDKSFGFSKSFASKYELGDEVGRGHFGYTCAAKFKKGDNK 176
Query: 160 GKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCE 219
G+ VAVK+I K+KMT+ + IEDVRREVKIL+ALSGH NL HF+ A+ED +N+YIVMELCE
Sbjct: 177 GQQVAVKVIPKAKMTTAIAIEDVRREVKILRALSGHNNLPHFYDAYEDHDNVYIVMELCE 236
Query: 220 GGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFM 279
GGELL+RI+SRGG+Y E+++KT+++QIL VVAFCHLQG+VHRDLKPE NFLF
Sbjct: 237 GGELLDRILSRGGKYTEEDAKTVMIQILNVVAFCHLQGVVHRDLKPE--------NFLFT 296
Query: 280 KKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTT 339
K++ + LK IDFGLSD+V+P
Sbjct: 297 SKEDTSQLKAIDFGLSDYVRP--------------------------------------- 356
Query: 340 PISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGI 399
DERLND+VGSAYYVAPEVL+RSYS EAD+WS+GVI YILLCGSRPFWARTESGI
Sbjct: 357 ------DERLNDIVGSAYYVAPEVLHRSYSTEADIWSVGVIVYILLCGSRPFWARTESGI 416
Query: 400 FRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRD-EDLAVPL 459
FR+VL+ADP+FDD PWP +S EA+DFVKRLLNKD RKR+TAAQAL+HPW++D D VP+
Sbjct: 417 FRAVLKADPSFDDPPWPLLSSEARDFVKRLLNKDPRKRLTAAQALSHPWIKDSNDAKVPM 476
Query: 460 DNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKAA 519
D ++K ++AYL ++ ++AAL+AL+K LT DELFYLR QF LL+P +NG ++++N K+A
Sbjct: 477 DILVFKLMRAYLRSSSLRKAALRALSKTLTVDELFYLREQFALLEPSKNGTISLENIKSA 536
Query: 520 LVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEY 579
L++ TDAM++S + + L + L +RM FEEFCAAA+SV+QLEA+ WE A A+E
Sbjct: 537 LMKMATDAMKDSRIPEFLGQLSALQYRRMDFEEFCAAALSVHQLEALDRWEQHARCAYEL 596
Query: 580 FEQDGNRVISVHELVQEMNLGPI--AHSFLQNWTRSSDGKLSFFGYTRFLHGVTIR 615
FE++GNR I + EL E+ LGP H+ L +W R +DGKLSF G+ + LHGV+ R
Sbjct: 597 FEKEGNRPIMIDELASELGLGPSVPVHAVLHDWLRHTDGKLSFLGFVKLLHGVSSR 596
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7016799.1 | 0.0e+00 | 100.00 | CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_022922250.1 | 0.0e+00 | 90.65 | CDPK-related kinase 4 isoform X2 [Cucurbita moschata] | [more] |
XP_023549645.1 | 0.0e+00 | 88.64 | CDPK-related kinase 6-like isoform X2 [Cucurbita pepo subsp. pepo] | [more] |
XP_022974330.1 | 1.2e-308 | 88.16 | CDPK-related kinase 6-like isoform X2 [Cucurbita maxima] | [more] |
KAG6579299.1 | 7.3e-293 | 90.33 | CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
Match Name | E-value | Identity | Description | |
Q9FIM9 | 9.5e-203 | 57.12 | CDPK-related kinase 4 OS=Arabidopsis thaliana OX=3702 GN=CRK4 PE=1 SV=1 | [more] |
Q9SG12 | 1.8e-201 | 57.21 | CDPK-related kinase 6 OS=Arabidopsis thaliana OX=3702 GN=CRK6 PE=1 SV=1 | [more] |
Q9ZUZ2 | 1.5e-200 | 61.17 | CDPK-related kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRK3 PE=1 SV=1 | [more] |
Q9SCS2 | 1.4e-177 | 55.20 | CDPK-related kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CRK5 PE=2 SV=1 | [more] |
P53681 | 2.6e-176 | 53.21 | CDPK-related protein kinase OS=Daucus carota OX=4039 GN=CRK PE=2 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1E874 | 0.0e+00 | 90.65 | CDPK-related kinase 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430287 P... | [more] |
A0A6J1IDP0 | 6.0e-309 | 88.16 | CDPK-related kinase 6-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147296... | [more] |
A0A6J1E2V0 | 4.8e-290 | 89.64 | CDPK-related kinase 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430287 P... | [more] |
A0A6J1IHA7 | 1.1e-281 | 87.16 | CDPK-related kinase 6-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147296... | [more] |
A0A6J1K6B2 | 2.0e-280 | 79.15 | CDPK-related kinase 6-like OS=Cucurbita maxima OX=3661 GN=LOC111491067 PE=4 SV=1 | [more] |