Carg23489 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23489
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionheat stress transcription factor A-6b-like
LocationCarg_Chr14: 12835035 .. 12835908 (+)
RNA-Seq ExpressionCarg23489
SyntenyCarg23489
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTTTTCCCGCCAGGTGAAGGAGGAGTTTCCGGCGTCGAGTTCTTCCTTTTCCGGCGGAGTTTTGCCACCCACGGCGACGCCAGCTCCTCCTCAGCCGATGGAGGGGCTTCACGAAGCAGGTCCGCCGCCATTTCTGACAAAAACATACGAAATTATCGAAGACACAGCCACCGATCATATTGTGTCTTGGAGCAGAGGCAACAATAGCTTCGTGGTTTGGGATCTTCAATCCTTTTCCTTAACCCTTCTTCCTAAATACTTCAAACACTGCAATTTCTCAAGCTTCGTTAGACAACTGAATACCTACGTAAGTCGATTTCAAATGGGTGTTGTTTTAATTCAACCAAATCAATAGTTCTATGTTTTTTTTCTGGTAGGGATTTAGAAAGGTGGATCCAGACAAGTGGGAATTCGCTCACGAAAGCTTCTTGCGAGGGCAGAGACATCTTCTGAAGCTGATTCGAAGGAGAAAAGCTCTGCAACCGAACGTTTCTCAGCAAGCTCCAGACCCATGTGTCGAGGTCGGACGGTTTGGGCTCGACGGAGAGGTCAACCAGCTGCAGCGTGACAAACAGGTATTAATGATGGAGGTAGTGAAGCTAAGACAACAGCAGCAAACCATGAAAACGTACCTCCGAACAATGGAACGGCGGCTGAAAAAAACAGAGACAAAACAACAACTAATGATGAACTTCTTGGCAAAAGCAATTCAAAACCCAGATTTCATCCAACACTTAATCCATCAAAAACACAAGAACAGAGAATTAGAGGACGCTATCAACCGGAAGCGGAGACGACGCATCGACCAAGGACAGCCGCCCGTCGTGAACGAAGTCGCCATTGATGATCAAATGAACCAAAAAGATAAG

mRNA sequence

ATGAATTTTTCCCGCCAGGTGAAGGAGGAGTTTCCGGCGTCGAGTTCTTCCTTTTCCGGCGGAGTTTTGCCACCCACGGCGACGCCAGCTCCTCCTCAGCCGATGGAGGGGCTTCACGAAGCAGGTCCGCCGCCATTTCTGACAAAAACATACGAAATTATCGAAGACACAGCCACCGATCATATTGTGTCTTGGAGCAGAGGCAACAATAGCTTCGTGGTTTGGGATCTTCAATCCTTTTCCTTAACCCTTCTTCCTAAATACTTCAAACACTGCAATTTCTCAAGCTTCGTTAGACAACTGAATACCTACGGATTTAGAAAGGTGGATCCAGACAAGTGGGAATTCGCTCACGAAAGCTTCTTGCGAGGGCAGAGACATCTTCTGAAGCTGATTCGAAGGAGAAAAGCTCTGCAACCGAACGTTTCTCAGCAAGCTCCAGACCCATGTGTCGAGGTCGGACGGTTTGGGCTCGACGGAGAGGTCAACCAGCTGCAGCGTGACAAACAGGTATTAATGATGGAGGTAGTGAAGCTAAGACAACAGCAGCAAACCATGAAAACGTACCTCCGAACAATGGAACGGCGGCTGAAAAAAACAGAGACAAAACAACAACTAATGATGAACTTCTTGGCAAAAGCAATTCAAAACCCAGATTTCATCCAACACTTAATCCATCAAAAACACAAGAACAGAGAATTAGAGGACGCTATCAACCGGAAGCGGAGACGACGCATCGACCAAGGACAGCCGCCCGTCGTGAACGAAGTCGCCATTGATGATCAAATGAACCAAAAAGATAAG

Coding sequence (CDS)

ATGAATTTTTCCCGCCAGGTGAAGGAGGAGTTTCCGGCGTCGAGTTCTTCCTTTTCCGGCGGAGTTTTGCCACCCACGGCGACGCCAGCTCCTCCTCAGCCGATGGAGGGGCTTCACGAAGCAGGTCCGCCGCCATTTCTGACAAAAACATACGAAATTATCGAAGACACAGCCACCGATCATATTGTGTCTTGGAGCAGAGGCAACAATAGCTTCGTGGTTTGGGATCTTCAATCCTTTTCCTTAACCCTTCTTCCTAAATACTTCAAACACTGCAATTTCTCAAGCTTCGTTAGACAACTGAATACCTACGGATTTAGAAAGGTGGATCCAGACAAGTGGGAATTCGCTCACGAAAGCTTCTTGCGAGGGCAGAGACATCTTCTGAAGCTGATTCGAAGGAGAAAAGCTCTGCAACCGAACGTTTCTCAGCAAGCTCCAGACCCATGTGTCGAGGTCGGACGGTTTGGGCTCGACGGAGAGGTCAACCAGCTGCAGCGTGACAAACAGGTATTAATGATGGAGGTAGTGAAGCTAAGACAACAGCAGCAAACCATGAAAACGTACCTCCGAACAATGGAACGGCGGCTGAAAAAAACAGAGACAAAACAACAACTAATGATGAACTTCTTGGCAAAAGCAATTCAAAACCCAGATTTCATCCAACACTTAATCCATCAAAAACACAAGAACAGAGAATTAGAGGACGCTATCAACCGGAAGCGGAGACGACGCATCGACCAAGGACAGCCGCCCGTCGTGAACGAAGTCGCCATTGATGATCAAATGAACCAAAAAGATAAG

Protein sequence

MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATDHIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHESFLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLRQQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINRKRRRRIDQGQPPVVNEVAIDDQMNQKDK
Homology
BLAST of Carg23489 vs. NCBI nr
Match: KAG6582330.1 (Heat stress transcription factor A-6b, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 532.7 bits (1371), Expect = 1.8e-147
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD 60
           MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD
Sbjct: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD 60

Query: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120
           HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES
Sbjct: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120

Query: 121 FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR 180
           FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR
Sbjct: 121 FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR 180

Query: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240
           QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR
Sbjct: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240

Query: 241 KRRRRIDQGQPPVVNEVAIDDQMNQKDK 269
           KRRRRIDQGQPPVVNEVAIDDQMNQKDK
Sbjct: 241 KRRRRIDQGQPPVVNEVAIDDQMNQKDK 268

BLAST of Carg23489 vs. NCBI nr
Match: KAG7018739.1 (Heat stress transcription factor A-6b, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 532.7 bits (1371), Expect = 1.8e-147
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD 60
           MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD
Sbjct: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD 60

Query: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120
           HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES
Sbjct: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120

Query: 121 FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR 180
           FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR
Sbjct: 121 FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR 180

Query: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240
           QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR
Sbjct: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240

Query: 241 KRRRRIDQGQPPVVNEVAIDDQMNQKDK 269
           KRRRRIDQGQPPVVNEVAIDDQMNQKDK
Sbjct: 241 KRRRRIDQGQPPVVNEVAIDDQMNQKDK 268

BLAST of Carg23489 vs. NCBI nr
Match: XP_022955849.1 (heat stress transcription factor A-6b-like [Cucurbita moschata])

HSP 1 Score: 514.6 bits (1324), Expect = 5.1e-142
Identity = 257/268 (95.90%), Postives = 263/268 (98.13%), Query Frame = 0

Query: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD 60
           MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYE IEDTATD
Sbjct: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEFIEDTATD 60

Query: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120
           HIVSWSRGNNSFVVWDLQSFSL+LLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES
Sbjct: 61  HIVSWSRGNNSFVVWDLQSFSLSLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120

Query: 121 FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR 180
           FLRGQRHLLKLIRRRKALQPNV  QAPDPC+EVGRFGLDGE N+LQRDKQVLM+EVVKLR
Sbjct: 121 FLRGQRHLLKLIRRRKALQPNVYHQAPDPCIEVGRFGLDGEANRLQRDKQVLMVEVVKLR 180

Query: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240
           QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR
Sbjct: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240

Query: 241 KRRRRIDQGQPPVVNEVAIDDQMNQKDK 269
           KRRRRIDQGQ PVVN+VA+DDQMNQKDK
Sbjct: 241 KRRRRIDQGQLPVVNDVALDDQMNQKDK 268

BLAST of Carg23489 vs. NCBI nr
Match: XP_023527179.1 (heat stress transcription factor A-6b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 509.2 bits (1310), Expect = 2.1e-140
Identity = 254/268 (94.78%), Postives = 262/268 (97.76%), Query Frame = 0

Query: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD 60
           MNFSRQVKEEFPASSSSFSGGVLPPTAT  PPQPMEG+HEAGPPPFLTKTYEIIEDTATD
Sbjct: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATAPPPQPMEGIHEAGPPPFLTKTYEIIEDTATD 60

Query: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120
           HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES
Sbjct: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120

Query: 121 FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR 180
           FLRGQRHLLKLIRRRKALQPNVS QAPDPCVEVGRFGLDG+VNQLQRDKQVLM+E+VKLR
Sbjct: 121 FLRGQRHLLKLIRRRKALQPNVSHQAPDPCVEVGRFGLDGDVNQLQRDKQVLMVEIVKLR 180

Query: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240
           QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKH++RELEDAIN 
Sbjct: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHRHRELEDAINP 240

Query: 241 KRRRRIDQGQPPVVNEVAIDDQMNQKDK 269
           KRRR IDQGQPPVVN VAIDDQMN+KD+
Sbjct: 241 KRRRHIDQGQPPVVNSVAIDDQMNRKDE 268

BLAST of Carg23489 vs. NCBI nr
Match: XP_022979505.1 (heat stress transcription factor A-6b-like [Cucurbita maxima])

HSP 1 Score: 475.7 bits (1223), Expect = 2.6e-130
Identity = 244/269 (90.71%), Postives = 253/269 (94.05%), Query Frame = 0

Query: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD 60
           MNF+RQVKEEFPASSS FSGGVLPPTAT APPQPMEGLH+ GPPPFLTKTYEIIED ATD
Sbjct: 1   MNFARQVKEEFPASSSPFSGGVLPPTATAAPPQPMEGLHKPGPPPFLTKTYEIIEDAATD 60

Query: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120
           HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES
Sbjct: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120

Query: 121 FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR 180
           FLRGQR LLKLIRRRKALQPNVS QAPDPCVEVGRFGLDGE N+LQRDKQVLM+EVVKLR
Sbjct: 121 FLRGQRRLLKLIRRRKALQPNVSHQAPDPCVEVGRFGLDGEANRLQRDKQVLMVEVVKLR 180

Query: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240
           QQQQT KTYLRTMER L+KTETKQQLMMNFLAKAIQNPDFIQHLIHQKHK+RELEDAIN 
Sbjct: 181 QQQQTTKTYLRTMERWLEKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKHRELEDAING 240

Query: 241 KRRRRIDQGQPPV-VNEVAIDDQMNQKDK 269
           K RR IDQGQ PV   ++AIDDQMNQKD+
Sbjct: 241 K-RRHIDQGQLPVRERQIAIDDQMNQKDE 268

BLAST of Carg23489 vs. ExPASy Swiss-Prot
Match: Q9LUH8 (Heat stress transcription factor A-6b OS=Arabidopsis thaliana OX=3702 GN=HSFA6b PE=2 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.3e-84
Identity = 160/270 (59.26%), Postives = 206/270 (76.30%), Query Frame = 0

Query: 5   RQVKEEFPAS---------------SSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTK 64
           R +KEEFPA                SSS +   +    T + PQP+EGLHE+GPPPFLTK
Sbjct: 6   RFIKEEFPAGFSDSPSPPSSSSYLYSSSMAEAAINDPTTLSYPQPLEGLHESGPPPFLTK 65

Query: 65  TYEIIEDTATDHIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKV 124
           TY+++ED+ T+H+VSWS+ NNSF+VWD Q+FS+TLLP++FKH NFSSFVRQLNTYGFRKV
Sbjct: 66  TYDLVEDSRTNHVVSWSKSNNSFIVWDPQAFSVTLLPRFFKHNNFSSFVRQLNTYGFRKV 125

Query: 125 DPDKWEFAHESFLRGQRHLLKLIRRRKALQPNVSQQAPDP---------CVEVGRFGLDG 184
           +PD+WEFA+E FLRGQ+HLLK IRRRK    +   Q P           C+EVGR+GLDG
Sbjct: 126 NPDRWEFANEGFLRGQKHLLKNIRRRKTSNNSNQMQQPQSSEQQSLDNFCIEVGRYGLDG 185

Query: 185 EVNQLQRDKQVLMMEVVKLRQQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDF 244
           E++ L+RDKQVLMME+V+LRQQQQ+ K YL  +E +LKKTE+KQ+ MM+FLA+A+QNPDF
Sbjct: 186 EMDSLRRDKQVLMMELVRLRQQQQSTKMYLTLIEEKLKKTESKQKQMMSFLARAMQNPDF 245

Query: 245 IQHLIHQKHKNRELEDAINRKRRRRIDQGQ 251
           IQ L+ QK K +E+E+AI++KR+R IDQG+
Sbjct: 246 IQQLVEQKEKRKEIEEAISKKRQRPIDQGK 275

BLAST of Carg23489 vs. ExPASy Swiss-Prot
Match: Q6VBB2 (Heat stress transcription factor A-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA2B PE=2 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.2e-74
Identity = 144/241 (59.75%), Postives = 185/241 (76.76%), Query Frame = 0

Query: 27  ATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATDHIVSWSRGNNSFVVWDLQSFSLTLLP 86
           A    P+PMEGLH+AGPPPFLTKTY++++D  TD  VSWS  +NSFVVWD  +F+  LLP
Sbjct: 34  AAGVAPRPMEGLHDAGPPPFLTKTYDMVDDAGTDAAVSWSATSNSFVVWDPHAFATVLLP 93

Query: 87  KYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHESFLRGQRHLLKLIRRRKALQPNVS-QQ 146
           ++FKH NFSSFVRQLNTYGFRKVDPD+WEFA+E+FLRGQRHLLK I+RRK      S QQ
Sbjct: 94  RFFKHNNFSSFVRQLNTYGFRKVDPDRWEFANENFLRGQRHLLKNIKRRKPPSHTASNQQ 153

Query: 147 APDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLRQQQQTMKTYLRTMERRLKKTETKQQ 206
           +  P +EVG FG D E+++L+RDKQ+LM EVVKLRQ+QQ  K  L+ ME RL+ TE +QQ
Sbjct: 154 SLGPYLEVGHFGYDAEIDRLKRDKQLLMAEVVKLRQEQQNTKANLKAMEDRLQGTEQRQQ 213

Query: 207 LMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINRKRRRRIDQGQPPVVNEVAIDDQMNQ 266
            MM FLA+ ++NP+F++ L+ Q    +EL+DAI++KRRRRIDQG  P V++V     + Q
Sbjct: 214 QMMAFLARVMKNPEFLKQLMSQNEMRKELQDAISKKRRRRIDQG--PEVDDVGTSSSIEQ 272

BLAST of Carg23489 vs. ExPASy Swiss-Prot
Match: Q338B0 (Heat stress transcription factor A-2c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA2C PE=1 SV=2)

HSP 1 Score: 277.7 bits (709), Expect = 1.4e-73
Identity = 140/220 (63.64%), Postives = 172/220 (78.18%), Query Frame = 0

Query: 30  APPQPMEGLHEAGPPPFLTKTYEIIEDTATDHIVSWSRGNNSFVVWDLQSFSLTLLPKYF 89
           A P+PMEGLHE GPPPFLTKTY+++ED ATD +VSWSR  NSFVVWD   F+  LLP+ F
Sbjct: 27  AAPRPMEGLHEVGPPPFLTKTYDLVEDPATDGVVSWSRAGNSFVVWDPHVFADLLLPRLF 86

Query: 90  KHCNFSSFVRQLNTYGFRKVDPDKWEFAHESFLRGQRHLLKLIRRRK--ALQPNVSQQAP 149
           KH NFSSFVRQLNTYGFRKVDPD+WEFA+E FLRGQRHLLK I+RRK  +  P   QQ+ 
Sbjct: 87  KHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQRHLLKTIKRRKPPSNAPPSQQQSL 146

Query: 150 DPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLRQQQQTMKTYLRTMERRLKKTETKQQLM 209
             C+EVG FG + E+++L+RDK +L+ EVVKLRQ+QQ  K +++ ME RL+  E KQ  M
Sbjct: 147 TSCLEVGEFGFEEEIDRLKRDKNILITEVVKLRQEQQATKDHVKAMEDRLRAAEQKQVQM 206

Query: 210 MNFLAKAIQNPDFIQHLIHQKHKNRELEDAINRKRRRRID 248
           M FLA+A++NP+F Q L  QK K +ELEDAI++KRRR ID
Sbjct: 207 MGFLARAMRNPEFFQQLAQQKEKRKELEDAISKKRRRPID 246

BLAST of Carg23489 vs. ExPASy Swiss-Prot
Match: Q6F388 (Heat stress transcription factor A-2e OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA2E PE=2 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.2e-72
Identity = 137/223 (61.43%), Postives = 173/223 (77.58%), Query Frame = 0

Query: 27  ATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATDHIVSWSRGNNSFVVWDLQSFSLTLLP 86
           A   PP+PM+GL + GPPPFLTKTY++++D  TD +VSWS  NNSFVVWD   F   LLP
Sbjct: 20  ANGQPPRPMDGLADGGPPPFLTKTYDMVDDPTTDAVVSWSATNNSFVVWDPHLFGNVLLP 79

Query: 87  KYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHESFLRGQRHLLKLIRRRKALQPNVSQQA 146
           +YFKH NFSSFVRQLNTYGFRKVDPDKWEFA+E FLRGQ+HLLK I+RRK    + SQQ+
Sbjct: 80  RYFKHNNFSSFVRQLNTYGFRKVDPDKWEFANEGFLRGQKHLLKSIKRRKPPNSSPSQQS 139

Query: 147 PDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLRQQQQTMKTYLRTMERRLKKTETKQQL 206
               +EVG FG +GE++QL+RDK +LM EVVKLRQ+QQ  K+ L+ ME++L+ TE KQQ 
Sbjct: 140 LGSFLEVGHFGYEGEIDQLKRDKHLLMAEVVKLRQEQQNTKSDLQAMEQKLQGTEQKQQH 199

Query: 207 MMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINRKRRRRIDQG 250
           MM FL++ + NP+FI+ L  Q    +ELE+ +++KRRRRIDQG
Sbjct: 200 MMAFLSRVMHNPEFIRQLFSQSEMRKELEEFVSKKRRRRIDQG 242

BLAST of Carg23489 vs. ExPASy Swiss-Prot
Match: Q8H7Y6 (Heat stress transcription factor A-2d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA2D PE=2 SV=2)

HSP 1 Score: 271.6 bits (693), Expect = 9.8e-72
Identity = 149/261 (57.09%), Postives = 188/261 (72.03%), Query Frame = 0

Query: 7   VKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATDHIVSWS 66
           VKEE+P SS         P    A P+PMEGLHE GPPPFLTKT++++ D ATD +VSW 
Sbjct: 9   VKEEWPPSS---------PEEGEA-PRPMEGLHEVGPPPFLTKTFDLVADPATDGVVSWG 68

Query: 67  RGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHESFLRGQR 126
           R  +SFVVWD   F+   LP++FKH NFSSFVRQLNTYGFRK+DPD+WEFA++ FLRGQR
Sbjct: 69  RAGSSFVVWDPHVFAAVFLPRFFKHNNFSSFVRQLNTYGFRKIDPDRWEFANDGFLRGQR 128

Query: 127 HLLKLIRRRKALQ--PNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLRQQQQ 186
           HLLK+I+RR+ L   P  SQQA   C+EVG+FGLD E+++L+RDK +L+ EVVKLR +QQ
Sbjct: 129 HLLKMIKRRRPLSYLPG-SQQALGTCLEVGQFGLDEEIDRLKRDKNILLAEVVKLRHKQQ 188

Query: 187 TMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINRKRRR 246
           + K  +R ME RL+  E KQ  MM FLA+A+QNPDF   LIHQ+ K + LED  ++KR R
Sbjct: 189 STKANMRAMEERLQHAEQKQVQMMGFLARAMQNPDFFHQLIHQQDKMKGLEDTFSKKRTR 248

Query: 247 RIDQGQPPVVN--EVAIDDQM 264
            ID    P +N  EV+  DQ+
Sbjct: 249 SID--IVPFLNPGEVSQGDQL 256

BLAST of Carg23489 vs. ExPASy TrEMBL
Match: A0A6J1GXF7 (heat stress transcription factor A-6b-like OS=Cucurbita moschata OX=3662 GN=LOC111457713 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.5e-142
Identity = 257/268 (95.90%), Postives = 263/268 (98.13%), Query Frame = 0

Query: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD 60
           MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYE IEDTATD
Sbjct: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEFIEDTATD 60

Query: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120
           HIVSWSRGNNSFVVWDLQSFSL+LLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES
Sbjct: 61  HIVSWSRGNNSFVVWDLQSFSLSLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120

Query: 121 FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR 180
           FLRGQRHLLKLIRRRKALQPNV  QAPDPC+EVGRFGLDGE N+LQRDKQVLM+EVVKLR
Sbjct: 121 FLRGQRHLLKLIRRRKALQPNVYHQAPDPCIEVGRFGLDGEANRLQRDKQVLMVEVVKLR 180

Query: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240
           QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR
Sbjct: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240

Query: 241 KRRRRIDQGQPPVVNEVAIDDQMNQKDK 269
           KRRRRIDQGQ PVVN+VA+DDQMNQKDK
Sbjct: 241 KRRRRIDQGQLPVVNDVALDDQMNQKDK 268

BLAST of Carg23489 vs. ExPASy TrEMBL
Match: A0A6J1ITG1 (heat stress transcription factor A-6b-like OS=Cucurbita maxima OX=3661 GN=LOC111479186 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.3e-130
Identity = 244/269 (90.71%), Postives = 253/269 (94.05%), Query Frame = 0

Query: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD 60
           MNF+RQVKEEFPASSS FSGGVLPPTAT APPQPMEGLH+ GPPPFLTKTYEIIED ATD
Sbjct: 1   MNFARQVKEEFPASSSPFSGGVLPPTATAAPPQPMEGLHKPGPPPFLTKTYEIIEDAATD 60

Query: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120
           HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES
Sbjct: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120

Query: 121 FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR 180
           FLRGQR LLKLIRRRKALQPNVS QAPDPCVEVGRFGLDGE N+LQRDKQVLM+EVVKLR
Sbjct: 121 FLRGQRRLLKLIRRRKALQPNVSHQAPDPCVEVGRFGLDGEANRLQRDKQVLMVEVVKLR 180

Query: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240
           QQQQT KTYLRTMER L+KTETKQQLMMNFLAKAIQNPDFIQHLIHQKHK+RELEDAIN 
Sbjct: 181 QQQQTTKTYLRTMERWLEKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKHRELEDAING 240

Query: 241 KRRRRIDQGQPPV-VNEVAIDDQMNQKDK 269
           K RR IDQGQ PV   ++AIDDQMNQKD+
Sbjct: 241 K-RRHIDQGQLPVRERQIAIDDQMNQKDE 268

BLAST of Carg23489 vs. ExPASy TrEMBL
Match: A0A0A0L5E2 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G146290 PE=3 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.1e-109
Identity = 210/257 (81.71%), Postives = 222/257 (86.38%), Query Frame = 0

Query: 5   RQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATDHIVS 64
           R+VKEE PAS SS        T T A PQPMEGLHEAGPPPFLTKTYEIIED  T+HIVS
Sbjct: 6   RRVKEEIPASGSS-------GTTTAASPQPMEGLHEAGPPPFLTKTYEIIEDIGTNHIVS 65

Query: 65  WSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHESFLRG 124
           WSRGNNSFVVWD QSFSLTLLPKYFKH NFSSFVRQLNTYGFRKVDPDKWEFAHE FLRG
Sbjct: 66  WSRGNNSFVVWDPQSFSLTLLPKYFKHSNFSSFVRQLNTYGFRKVDPDKWEFAHEGFLRG 125

Query: 125 QRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLRQQQQ 184
           Q+HLLKLIRRRKA QPN S QAPD CVEVGRFGLDGEV++LQRDKQVLM EVVKLRQQQQ
Sbjct: 126 QKHLLKLIRRRKASQPNASHQAPDSCVEVGRFGLDGEVDRLQRDKQVLMAEVVKLRQQQQ 185

Query: 185 TMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINRKRRR 244
             KTYL+TMERRLKKTETKQQLMMNFLA+AIQNPDFIQ LIHQK K++ELE+ INRKRRR
Sbjct: 186 NTKTYLQTMERRLKKTETKQQLMMNFLARAIQNPDFIQQLIHQKDKHKELEETINRKRRR 245

Query: 245 RIDQGQPPVVNEVAIDD 262
            IDQGQP   +E   +D
Sbjct: 246 HIDQGQPDFEDEEEKED 255

BLAST of Carg23489 vs. ExPASy TrEMBL
Match: A0A1S3AWX8 (heat stress transcription factor A-6b OS=Cucumis melo OX=3656 GN=LOC103483673 PE=3 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 2.3e-108
Identity = 208/257 (80.93%), Postives = 223/257 (86.77%), Query Frame = 0

Query: 5   RQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATDHIVS 64
           R+VKEEF ASSSS          T A PQP+EGLHEAGPPPFLTKTYEIIED  T+HIVS
Sbjct: 6   RRVKEEFTASSSS--------RTTTASPQPLEGLHEAGPPPFLTKTYEIIEDIGTNHIVS 65

Query: 65  WSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHESFLRG 124
           WSRGNNSFVVWD QSFSLTLLPKYFKH NFSSFVRQLNTYGFRKVDPDKWEFAHE FLRG
Sbjct: 66  WSRGNNSFVVWDPQSFSLTLLPKYFKHSNFSSFVRQLNTYGFRKVDPDKWEFAHEGFLRG 125

Query: 125 QRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLRQQQQ 184
           Q+HLLKLIRRRKA QPN S QAPDPCVEVGRFGLDGEV++LQRDKQVLM EVVKLRQQQQ
Sbjct: 126 QKHLLKLIRRRKASQPNASHQAPDPCVEVGRFGLDGEVDRLQRDKQVLMAEVVKLRQQQQ 185

Query: 185 TMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINRKRRR 244
             KT L+TMERRLKKTET+QQLMMNFLA+AIQNPDFIQ LIHQK K++ELE+AINRKRRR
Sbjct: 186 NTKTCLQTMERRLKKTETRQQLMMNFLARAIQNPDFIQQLIHQKDKHKELEEAINRKRRR 245

Query: 245 RIDQGQPPVVNEVAIDD 262
            IDQG+P   +E   +D
Sbjct: 246 HIDQGRPDFEDEEEKED 254

BLAST of Carg23489 vs. ExPASy TrEMBL
Match: A0A6J1E1I0 (heat stress transcription factor A-6b-like OS=Cucurbita moschata OX=3662 GN=LOC111429716 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 3.0e-108
Identity = 203/262 (77.48%), Postives = 227/262 (86.64%), Query Frame = 0

Query: 1   MNFSRQVKEEFPASSSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATD 60
           MN  R+VKEEFPAS+S+FS G   PT     PQPMEGLHEAGPPPFLTKTYE +ED+ T+
Sbjct: 1   MNRLRRVKEEFPASNSAFSNGAPSPTVA---PQPMEGLHEAGPPPFLTKTYEFVEDSTTN 60

Query: 61  HIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHES 120
           HIVSWSR NNSF+VWD QSFSLTLLPKYFKH NFSSFVRQLNTYGFRK+DPDKWEFAHE 
Sbjct: 61  HIVSWSRDNNSFIVWDPQSFSLTLLPKYFKHSNFSSFVRQLNTYGFRKIDPDKWEFAHEG 120

Query: 121 FLRGQRHLLKLIRRRKALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLR 180
           FLRGQ+HLLKLIRRRK +QPN + QAPDPCVEVGRFGLDGE+++LQRDKQVLM E+VKLR
Sbjct: 121 FLRGQKHLLKLIRRRKTVQPNATLQAPDPCVEVGRFGLDGEIDRLQRDKQVLMGELVKLR 180

Query: 181 QQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINR 240
           QQQQT K  L+TMERRLKKTET+QQLMMNFLA+AIQ PDFIQ LIHQK K++ELE+AINR
Sbjct: 181 QQQQTTKGQLQTMERRLKKTETRQQLMMNFLARAIQFPDFIQQLIHQKDKHKELEEAINR 240

Query: 241 KRRRRIDQGQPPVVNEVAIDDQ 263
           KRRR IDQG  P  +E   +D+
Sbjct: 241 KRRRHIDQGLSPTSDEYDEEDK 259

BLAST of Carg23489 vs. TAIR 10
Match: AT3G22830.1 (heat shock transcription factor A6B )

HSP 1 Score: 314.3 bits (804), Expect = 9.4e-86
Identity = 160/270 (59.26%), Postives = 206/270 (76.30%), Query Frame = 0

Query: 5   RQVKEEFPAS---------------SSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTK 64
           R +KEEFPA                SSS +   +    T + PQP+EGLHE+GPPPFLTK
Sbjct: 6   RFIKEEFPAGFSDSPSPPSSSSYLYSSSMAEAAINDPTTLSYPQPLEGLHESGPPPFLTK 65

Query: 65  TYEIIEDTATDHIVSWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKV 124
           TY+++ED+ T+H+VSWS+ NNSF+VWD Q+FS+TLLP++FKH NFSSFVRQLNTYGFRKV
Sbjct: 66  TYDLVEDSRTNHVVSWSKSNNSFIVWDPQAFSVTLLPRFFKHNNFSSFVRQLNTYGFRKV 125

Query: 125 DPDKWEFAHESFLRGQRHLLKLIRRRKALQPNVSQQAPDP---------CVEVGRFGLDG 184
           +PD+WEFA+E FLRGQ+HLLK IRRRK    +   Q P           C+EVGR+GLDG
Sbjct: 126 NPDRWEFANEGFLRGQKHLLKNIRRRKTSNNSNQMQQPQSSEQQSLDNFCIEVGRYGLDG 185

Query: 185 EVNQLQRDKQVLMMEVVKLRQQQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDF 244
           E++ L+RDKQVLMME+V+LRQQQQ+ K YL  +E +LKKTE+KQ+ MM+FLA+A+QNPDF
Sbjct: 186 EMDSLRRDKQVLMMELVRLRQQQQSTKMYLTLIEEKLKKTESKQKQMMSFLARAMQNPDF 245

Query: 245 IQHLIHQKHKNRELEDAINRKRRRRIDQGQ 251
           IQ L+ QK K +E+E+AI++KR+R IDQG+
Sbjct: 246 IQQLVEQKEKRKEIEEAISKKRQRPIDQGK 275

BLAST of Carg23489 vs. TAIR 10
Match: AT2G26150.1 (heat shock transcription factor A2 )

HSP 1 Score: 258.1 bits (658), Expect = 8.0e-69
Identity = 137/243 (56.38%), Postives = 177/243 (72.84%), Query Frame = 0

Query: 6   QVKEEFPASSSSFSGGVLPPTA--TPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATDHIV 65
           ++K E    + +F+G V   ++  + + P+PMEGL+E GPPPFLTKTYE++ED ATD +V
Sbjct: 3   ELKVEMEEETVTFTGSVAASSSVGSSSSPRPMEGLNETGPPPFLTKTYEMVEDPATDTVV 62

Query: 66  SWSRGNNSFVVWDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHESFLR 125
           SWS G NSFVVWD   FS TLLP+YFKH NFSSF+RQLNTYGFRK+DPD+WEFA+E FL 
Sbjct: 63  SWSNGRNSFVVWDSHKFSTTLLPRYFKHSNFSSFIRQLNTYGFRKIDPDRWEFANEGFLA 122

Query: 126 GQRHLLKLIRRRKAL-QPNVSQQAPD-PCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLRQ 185
           GQ+HLLK I+RR+ +   NV+QQ     CVEVG++G DGEV +L+RD  VL+ EVV+LRQ
Sbjct: 123 GQKHLLKNIKRRRNMGLQNVNQQGSGMSCVEVGQYGFDGEVERLKRDHGVLVAEVVRLRQ 182

Query: 186 QQQTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHL-IHQKHKNRELEDAINR 244
           QQ + K+ +  ME+RL  TE +QQ MM FLAKA+ NP+F+Q   +  K K       + R
Sbjct: 183 QQHSSKSQVAAMEQRLLVTEKRQQQMMTFLAKALNNPNFVQQFAVMSKEKKSLFGLDVGR 242

BLAST of Carg23489 vs. TAIR 10
Match: AT3G51910.1 (heat shock transcription factor A7A )

HSP 1 Score: 248.1 bits (632), Expect = 8.3e-66
Identity = 127/217 (58.53%), Postives = 164/217 (75.58%), Query Frame = 0

Query: 29  PAPPQPMEGLHEAGPPPFLTKTYEIIEDTATDHIVSWSRGNNSFVVWDLQSFSLTLLPKY 88
           P PPQPMEGLHE  PPPFLTKT+E+++D  TDHIVSW+RG  SFVVWDL SFS  LLP++
Sbjct: 13  PPPPQPMEGLHENAPPPFLTKTFEMVDDPNTDHIVSWNRGGTSFVVWDLHSFSTILLPRH 72

Query: 89  FKHCNFSSFVRQLNTYGFRKVDPDKWEFAHESFLRGQRHLLKLIRRRKALQPNVSQQAPD 148
           FKH NFSSF+RQLNTYGFRK++ ++WEFA+E FL GQR LLK I+RR    P+ S  + D
Sbjct: 73  FKHSNFSSFIRQLNTYGFRKIEAERWEFANEEFLLGQRQLLKNIKRRNPFTPS-SSPSHD 132

Query: 149 PCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLRQQQQTMKTYLRTMERRLKKTETKQQLMM 208
            C            N+L+R+KQVLMME+V LRQQQQT K+Y++ ME+R++ TE KQ+ MM
Sbjct: 133 AC------------NELRREKQVLMMEIVSLRQQQQTTKSYIKAMEQRIEGTERKQRQMM 192

Query: 209 NFLAKAIQNPDFIQHLIHQKHKN-RELEDAINRKRRR 245
           +FLA+A+Q+P F+  L+ Q+ K  +ELED  + KR+R
Sbjct: 193 SFLARAMQSPSFLHQLLKQRDKKIKELEDNESAKRKR 216

BLAST of Carg23489 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 232.3 bits (591), Expect = 4.7e-61
Identity = 132/240 (55.00%), Postives = 162/240 (67.50%), Query Frame = 0

Query: 24  PPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATDHIVSWSRGNNSFVVWDLQSFSLT 83
           PP   P P   +     + PPPFL+KTY+++ED ATD IVSWS  NNSF+VWD   FS  
Sbjct: 33  PPPRNPHPATLLNA--NSLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRD 92

Query: 84  LLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHESFLRGQRHLLKLIRRRKALQPN-- 143
           LLPKYFKH NFSSFVRQLNTYGFRKVDPD+WEFA+E FLRGQ+HLLK I RRK++Q +  
Sbjct: 93  LLPKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGS 152

Query: 144 -----VSQQ---------APDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLRQQQQTMK 203
                 SQQ         A   CVEVG+FGL+ EV QL+RDK VLM E+VKLRQQQQT  
Sbjct: 153 SSSNPQSQQLSQGQGSMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTD 212

Query: 204 TYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHKNRELEDAINRKRRRRID 248
             L+ + + L+  E +QQ +M+FLAKA+QNP F+   I ++  +       N+KRR R D
Sbjct: 213 NKLQVLVKHLQVMEQRQQQIMSFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRLRED 270

BLAST of Carg23489 vs. TAIR 10
Match: AT1G32330.1 (heat shock transcription factor A1D )

HSP 1 Score: 226.9 bits (577), Expect = 2.0e-59
Identity = 124/246 (50.41%), Postives = 163/246 (66.26%), Query Frame = 0

Query: 15  SSSFSGGVLPPTATPAPPQPMEGLHEAGPPPFLTKTYEIIEDTATDHIVSWSRGNNSFVV 74
           ++S  GG    T     PQP   L    PPPFL+KTY++++D  TD IVSWS  NNSF+V
Sbjct: 7   TTSDGGGDSMETKPSPQPQPAAILSSNAPPPFLSKTYDMVDDHNTDSIVSWSANNNSFIV 66

Query: 75  WDLQSFSLTLLPKYFKHCNFSSFVRQLNTYGFRKVDPDKWEFAHESFLRGQRHLLKLIRR 134
           W    F+  LLPK FKH NFSSFVRQLNTYGFRKVDPD+WEFA+E FLRGQ+HLL+ I R
Sbjct: 67  WKPPEFARDLLPKNFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLQSITR 126

Query: 135 RK-----------ALQPNVSQQAPDPCVEVGRFGLDGEVNQLQRDKQVLMMEVVKLRQQQ 194
           RK           +   N    +   CVEVG+FGL+ EV +L+RDK VLM E+V+LRQQQ
Sbjct: 127 RKPAHGQGQGHQRSQHSNGQNSSVSACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQ 186

Query: 195 QTMKTYLRTMERRLKKTETKQQLMMNFLAKAIQNPDFIQHLIHQKHK----NRELEDAIN 246
           Q+    L+TM +RL+  E +QQ +M+FLAKA+Q+P F+   + Q+++    NR + D   
Sbjct: 187 QSTDNQLQTMVQRLQGMENRQQQLMSFLAKAVQSPHFLSQFLQQQNQQNESNRRISDTSK 246

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6582330.11.8e-147100.00Heat stress transcription factor A-6b, partial [Cucurbita argyrosperma subsp. so... [more]
KAG7018739.11.8e-147100.00Heat stress transcription factor A-6b, partial [Cucurbita argyrosperma subsp. ar... [more]
XP_022955849.15.1e-14295.90heat stress transcription factor A-6b-like [Cucurbita moschata][more]
XP_023527179.12.1e-14094.78heat stress transcription factor A-6b-like [Cucurbita pepo subsp. pepo][more]
XP_022979505.12.6e-13090.71heat stress transcription factor A-6b-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LUH81.3e-8459.26Heat stress transcription factor A-6b OS=Arabidopsis thaliana OX=3702 GN=HSFA6b ... [more]
Q6VBB21.2e-7459.75Heat stress transcription factor A-2b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q338B01.4e-7363.64Heat stress transcription factor A-2c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6F3881.2e-7261.43Heat stress transcription factor A-2e OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q8H7Y69.8e-7257.09Heat stress transcription factor A-2d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Match NameE-valueIdentityDescription
A0A6J1GXF72.5e-14295.90heat stress transcription factor A-6b-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1ITG11.3e-13090.71heat stress transcription factor A-6b-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0L5E22.1e-10981.71HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G146290 ... [more]
A0A1S3AWX82.3e-10880.93heat stress transcription factor A-6b OS=Cucumis melo OX=3656 GN=LOC103483673 PE... [more]
A0A6J1E1I03.0e-10877.48heat stress transcription factor A-6b-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT3G22830.19.4e-8659.26heat shock transcription factor A6B [more]
AT2G26150.18.0e-6956.38heat shock transcription factor A2 [more]
AT3G51910.18.3e-6658.53heat shock transcription factor A7A [more]
AT4G17750.14.7e-6155.00heat shock factor 1 [more]
AT1G32330.12.0e-5950.41heat shock transcription factor A1D [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 162..182
NoneNo IPR availableCOILSCoilCoilcoord: 224..244
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..39
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..42
NoneNo IPR availablePANTHERPTHR10015:SF334HEAT STRESS TRANSCRIPTION FACTOR A-6Bcoord: 9..264
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 84..96
score: 76.7
coord: 97..109
score: 87.03
coord: 46..69
score: 41.31
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 42..135
e-value: 1.5E-58
score: 210.5
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 46..135
e-value: 2.7E-31
score: 108.0
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 85..109
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 38..136
e-value: 4.0E-36
score: 125.6
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 9..264
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 42..135

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23489-RACarg23489-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034605 cellular response to heat
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding