Carg20449 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20449
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description65-kDa microtubule-associated protein 8
LocationCarg_Chr12: 307228 .. 309431 (+)
RNA-Seq ExpressionCarg20449
SyntenyCarg20449
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAAATAAAAGATTTAATGAATTAGGTGCTAATTATGTGAAAAAAAAAAAAAAAAATTTCAAGGCTGAGGCTGAAGTCAAGAGACTTGATCAACTGAAAGCAAGCATGATGAAAGAGCTATTTATCAAGAAACAGAAAGAGCTGGAAGATATATGCAACAAATCGCACATGGAGATACCTTCAAGATTAGAAATGGAAAAGATCATAAATCTTATAAATTCTGGTACAGTGCGTAATGACATCAATGATAGGGAATATCATATACTTTATGCAAATCTATTATCCAGATACTAACTTCTATGTACTGCCAAAGGGGAAATTGATCATGTCAATCTCCTAACAAGCATGGATGAACAAATATCAAGAGCAAAAGAAGAAGCATCTAGCCGGAAGACTATAATGGAGAAAGTTGAGAAATGGATGCTAGCATGTGATGAAGAGCGGTGGTTGGAAGAATATAGCATGGTTGGTAATTTTCCTGCAAGCTATAAAAGACAATTACTTGACTGAAATCTTAGTCATAAGTAGTAATTATTGAGCTTTCAGGACCAAAGCCGATACTCAGTCAGCAGAGGTGCTCACAAGAATCTCAGACGTGCAGAACGAGCAAGAATAACAGTCAACAAAATTCCAGGTAATATCAGCAATAATTTCCATGAACTGCAAGGAAGGCTTTAAAGATGGGCTGTGTTAAATTGGCCAGACCATCTTTCGGTACAATATCTTTCCAAGCCAAAGAGATGTGAGCTTCTTGAAATTTGAACATATAAATAGGATTTCATATGACTTCATTATTTTTCTCCTCTTGAAAATTATGTAAACTGCAGCTTTGGTGGAATTGCTCTTGGCAAAGACTAAGAGCTGGGAGGCAGAACGAAGTAAAGTGTTCTTGTATGATGAGGTAAGCTATATAATGCCTTTCAACAATGCTGTTCTTGTCACGTGCTAATCATCATTTGAGAATATGATTATAAGTTTCATGAATGATATTCTAAAAGCATGCAACTAAGAACCTAATACGGTGAGATATTCGATAAACCCTATTAGATATGGTTTTTAAAACGTGTCTGCTAGGGAGAGATTTTCACATCCTTATAAAGAATGTTTCGTTCGCCTCTCCAACCGATATAGGGTCTCACAATCCGCCCCTTTCGGGACTCAGCATCCTCGCTGGCACTCGTTCCTCTCTCCAATCGATTTGGTACCCCCAATATTGTGAGATCCCACGTCGGTTGGAGAGGAGAATGAAACATTCTTTAAAAGAGTGTGAAAATCTCTCCCTAATAGACGCATTTTAAAAATCTTGAAAAAGTCCGGAAGAAACACCCGAAAGAAGACAATATCTGCTAGCGGTGGGCGTGAGCCATCACAACATCCTCGCCTTCTCCATAAAATATGTATATAAAAAGGAACCCAGTTCTTCTTTGCATCTCCCTCCATCCTAAAAATACCAAATTATCCGTTACTTTCGTGAAGACATGTGTCCTATCTTCAGGTGCCTTTGCTAGCAATGCTGGAGGAATATAATGCCCTGAGACAGGAAAAAGAGGAAGAGAAGATGAGACTAAGAGTAAGTACAACGTTACCCTGCTGCAGACTTTTAAATATGGATCATTAACAACTTCTTTCCCCATAACCCCATCACATAATTTGAACTAATCAGCAGAAAAAAGCTTTAGCAATGGTCTTTAGACGGTTAATGATAATTTTTCTAGCAGGAGAAGAAAAAATTTCAAACCCAGGTAGCAATCGAGCCAGAAAGCTTCTATGGATCAAGACCAAGCACCAGCAGTCGGCGTCTTTCAAATGCTAGCATTAATGGAGGTTATAGCAATGCCATAACTTTGAACAGAAGGCTTTCTCTAGGAATCCAGCAATTGGGATCAAACAGCACAAATTCAGCAAATGTAGGCACCTCTTTCATGAAGGAAGGGAAAACCTCACCTGAACAGCATACGCCTCTTCGGCACAACCTTGTTTCTCAGATCAGAGATGAAACAGCTTCAGTGGTATCCACATTTTCTGGCCCCATATCTCCTTAGAGTTCACTACACGACTTCAAGCTCAAATGTCATTCATAAAGATAATCCCACAGAATAAAGTTTGAGAGAGCCATTGTAGCCTTCAAATAAGCTTATGTCTTAACAGAGGATAGAGAAGATACTCGAGTATTTATTCTATCTTTGAATTTCCTTTTTATT

mRNA sequence

ATGGTAAATAAAAGATTTAATGAATTAGGTGCTAATTATGTGAAAAAAAAAAAAAAAAATTTCAAGGCTGAGGCTGAAGTCAAGAGACTTGATCAACTGAAAGCAAGCATGATGAAAGAGCTATTTATCAAGAAACAGAAAGAGCTGGAAGATATATGCAACAAATCGCACATGGAGATACCTTCAAGATTAGAAATGGAAAAGATCATAAATCTTATAAATTCTGGGGAAATTGATCATGTCAATCTCCTAACAAGCATGGATGAACAAATATCAAGAGCAAAAGAAGAAGCATCTAGCCGGAAGACTATAATGGAGAAAGTTGAGAAATGGATGCTAGCATGTGATGAAGAGCGGTGGTTGGAAGAATATAGCATGGACCAAAGCCGATACTCAGTCAGCAGAGGTGCTCACAAGAATCTCAGACGTGCAGAACGAGCAAGAATAACAGTCAACAAAATTCCAGCTTTGGTGGAATTGCTCTTGGCAAAGACTAAGAGCTGGGAGGCAGAACGAAGTAAAGTGTTCTTGTATGATGAGGTGCCTTTGCTAGCAATGCTGGAGGAATATAATGCCCTGAGACAGGAAAAAGAGGAAGAGAAGATGAGACTAAGAGAGAAGAAAAAATTTCAAACCCAGGTAGCAATCGAGCCAGAAAGCTTCTATGGATCAAGACCAAGCACCAGCAGTCGGCGTCTTTCAAATGCTAGCATTAATGGAGGTTATAGCAATGCCATAACTTTGAACAGAAGGCTTTCTCTAGGAATCCAGCAATTGGGATCAAACAGCACAAATTCAGCAAATGTAGGCACCTCTTTCATGAAGGAAGGGAAAACCTCACCTGAACAGCATACGCCTCTTCGGCACAACCTTGTTTCTCAGATCAGAGATGAAACAGCTTCAGTGGTATCCACATTTTCTGGCCCCATATCTCCTTAGAGTTCACTACACGACTTCAAGCTCAAATGTCATTCATAAAGATAATCCCACAGAATAAAGTTTGAGAGAGCCATTGTAGCCTTCAAATAAGCTTATGTCTTAACAGAGGATAGAGAAGATACTCGAGTATTTATTCTATCTTTGAATTTCCTTTTTATT

Coding sequence (CDS)

ATGGTAAATAAAAGATTTAATGAATTAGGTGCTAATTATGTGAAAAAAAAAAAAAAAAATTTCAAGGCTGAGGCTGAAGTCAAGAGACTTGATCAACTGAAAGCAAGCATGATGAAAGAGCTATTTATCAAGAAACAGAAAGAGCTGGAAGATATATGCAACAAATCGCACATGGAGATACCTTCAAGATTAGAAATGGAAAAGATCATAAATCTTATAAATTCTGGGGAAATTGATCATGTCAATCTCCTAACAAGCATGGATGAACAAATATCAAGAGCAAAAGAAGAAGCATCTAGCCGGAAGACTATAATGGAGAAAGTTGAGAAATGGATGCTAGCATGTGATGAAGAGCGGTGGTTGGAAGAATATAGCATGGACCAAAGCCGATACTCAGTCAGCAGAGGTGCTCACAAGAATCTCAGACGTGCAGAACGAGCAAGAATAACAGTCAACAAAATTCCAGCTTTGGTGGAATTGCTCTTGGCAAAGACTAAGAGCTGGGAGGCAGAACGAAGTAAAGTGTTCTTGTATGATGAGGTGCCTTTGCTAGCAATGCTGGAGGAATATAATGCCCTGAGACAGGAAAAAGAGGAAGAGAAGATGAGACTAAGAGAGAAGAAAAAATTTCAAACCCAGGTAGCAATCGAGCCAGAAAGCTTCTATGGATCAAGACCAAGCACCAGCAGTCGGCGTCTTTCAAATGCTAGCATTAATGGAGGTTATAGCAATGCCATAACTTTGAACAGAAGGCTTTCTCTAGGAATCCAGCAATTGGGATCAAACAGCACAAATTCAGCAAATGTAGGCACCTCTTTCATGAAGGAAGGGAAAACCTCACCTGAACAGCATACGCCTCTTCGGCACAACCTTGTTTCTCAGATCAGAGATGAAACAGCTTCAGTGGTATCCACATTTTCTGGCCCCATATCTCCTTAG

Protein sequence

MVNKRFNELGANYVKKKKKNFKAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHVNLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNLRRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEKMRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGSNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP
Homology
[Close]
BLAST of Carg20449 vs. NCBI nr
Match: KAG7020141.1 (65-kDa microtubule-associated protein 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582.8 bits (1501), Expect = 1.8e-162
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0 

Query: 1   MVNKRFNELGANYVKKKKKNFKAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEI 60
           MVNKRFNELGANYVKKKKKNFKAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEI
Sbjct: 1   MVNKRFNELGANYVKKKKKNFKAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEI 60

Query: 61  PSRLEMEKIINLINSGEIDHVNLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERW 120
           PSRLEMEKIINLINSGEIDHVNLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERW
Sbjct: 61  PSRLEMEKIINLINSGEIDHVNLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERW 120

Query: 121 LEEYSMDQSRYSVSRGAHKNLRRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDE 180
           LEEYSMDQSRYSVSRGAHKNLRRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDE
Sbjct: 121 LEEYSMDQSRYSVSRGAHKNLRRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDE 180

Query: 181 VPLLAMLEEYNALRQEKEEEKMRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASING 240
           VPLLAMLEEYNALRQEKEEEKMRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASING
Sbjct: 181 VPLLAMLEEYNALRQEKEEEKMRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASING 240

Query: 241 GYSNAITLNRRLSLGIQQLGSNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETA 300
           GYSNAITLNRRLSLGIQQLGSNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETA
Sbjct: 241 GYSNAITLNRRLSLGIQQLGSNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETA 300

Query: 301 SVVSTFSGPISP 313
           SVVSTFSGPISP
Sbjct: 301 SVVSTFSGPISP 312

[Close]
BLAST of Carg20449 vs. NCBI nr
Match: KAG6585221.1 (65-kDa microtubule-associated protein 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 541.6 bits (1394), Expect = 4.5e-150
Identity = 290/291 (99.66%), Postives = 291/291 (100.00%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV
Sbjct: 303 QAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 362

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
           NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL
Sbjct: 363 NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 422

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK
Sbjct: 423 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 482

Query: 202 MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS 261
           MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS
Sbjct: 483 MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS 542

Query: 262 NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 313
           NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP
Sbjct: 543 NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 593

[Close]
BLAST of Carg20449 vs. NCBI nr
Match: XP_022951089.1 (65-kDa microtubule-associated protein 8-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 541.6 bits (1394), Expect = 4.5e-150
Identity = 290/291 (99.66%), Postives = 291/291 (100.00%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV
Sbjct: 303 QAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 362

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
           NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL
Sbjct: 363 NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 422

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK
Sbjct: 423 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 482

Query: 202 MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS 261
           MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS
Sbjct: 483 MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS 542

Query: 262 NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 313
           NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP
Sbjct: 543 NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 593

[Close]
BLAST of Carg20449 vs. NCBI nr
Match: XP_023537211.1 (65-kDa microtubule-associated protein 8-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 539.3 bits (1388), Expect = 2.2e-149
Identity = 289/291 (99.31%), Postives = 290/291 (99.66%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV
Sbjct: 305 QAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 364

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
           NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL
Sbjct: 365 NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 424

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK
Sbjct: 425 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 484

Query: 202 MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS 261
           MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS
Sbjct: 485 MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS 544

Query: 262 NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 313
           NSTNSANV TSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP
Sbjct: 545 NSTNSANVSTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 595

[Close]
BLAST of Carg20449 vs. NCBI nr
Match: XP_022951088.1 (65-kDa microtubule-associated protein 8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 537.0 bits (1382), Expect = 1.1e-148
Identity = 290/292 (99.32%), Postives = 291/292 (99.66%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV
Sbjct: 303 QAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 362

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
           NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL
Sbjct: 363 NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 422

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK
Sbjct: 423 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 482

Query: 202 MRLR-EKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLG 261
           MRLR EKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLG
Sbjct: 483 MRLRQEKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLG 542

Query: 262 SNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 313
           SNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP
Sbjct: 543 SNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 594

[Close]
BLAST of Carg20449 vs. ExPASy Swiss-Prot
Match: Q9C7G0 (65-kDa microtubule-associated protein 8 OS=Arabidopsis thaliana OX=3702 GN=MAP65-8 PE=1 SV=2)

HSP 1 Score: 300.4 bits (768), Expect = 2.3e-80
Identity = 167/260 (64.23%), Postives = 204/260 (78.46%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AEAEVKRLDQLKAS +KELFIKKQKELED CN SHME PS  EM  I NL++SGE+DHV
Sbjct: 305 QAEAEVKRLDQLKASRIKELFIKKQKELEDTCNMSHMETPS-TEMGNITNLVDSGEVDHV 364

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
           +LL +MDE+I+RAKEEA+SRK I+EKV++WMLA DEERWLEEY  D++RYSVSR AH+NL
Sbjct: 365 DLLAAMDEKIARAKEEAASRKGIIEKVDRWMLASDEERWLEEYDQDENRYSVSRNAHRNL 424

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           RRAERARITV+KI  LVE +L K KSWE ER KVFLY+EVPL+AML+EYN LRQEKE EK
Sbjct: 425 RRAERARITVSKISGLVESILVKAKSWEVERQKVFLYNEVPLVAMLQEYNKLRQEKEMEK 484

Query: 202 MRLREKKKFQ-TQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLG 261
            RLRE KK    Q   E ++FY +RP++S+RR+SN S+NGG+ +   +NR+ S G     
Sbjct: 485 QRLREMKKMSIPQPVAEGDNFYMARPASSNRRISNRSMNGGFGSGSPINRKYSGGFNNTN 544

Query: 262 SNSTNSANVGTSFMKEGKTS 281
           +N T    +GTS  +E + S
Sbjct: 545 NNYT---ALGTSIRRESRKS 560

[Close]
BLAST of Carg20449 vs. ExPASy Swiss-Prot
Match: Q9FLP0 (65-kDa microtubule-associated protein 1 OS=Arabidopsis thaliana OX=3702 GN=MAP65-1 PE=1 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.4e-64
Identity = 141/247 (57.09%), Postives = 180/247 (72.87%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AE EV RLDQLKAS MKE+  KKQ ELE+I  ++H+E+      E+I++LI+SG ++  
Sbjct: 298 QAEVEVDRLDQLKASRMKEIAFKKQSELEEIYARAHVEVNPESARERIMSLIDSGNVEPT 357

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
            LL  MD QIS+AKEEA SRK I+++VEKWM AC+EE WLE+Y+ DQ+RYS SRGAH NL
Sbjct: 358 ELLADMDSQISKAKEEAFSRKDILDRVEKWMSACEEESWLEDYNRDQNRYSASRGAHLNL 417

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           +RAE+ARI V+KIPA+V+ L+AKT++WE E S  F YD VPLLAML+EY  LRQE+EEEK
Sbjct: 418 KRAEKARILVSKIPAMVDTLVAKTRAWEEEHSMSFAYDGVPLLAMLDEYGMLRQEREEEK 477

Query: 202 MRLREKKKFQTQVAIEPESFYGSRPS----TSSRRLSNASINGGYSNAITLNRRLSLGIQ 261
            RLRE+KK Q Q  +E ES + +RPS     S+++      N G +N  T NRRLSL   
Sbjct: 478 RRLREQKKVQEQPHVEQESAFSTRPSPARPVSAKKTVGPRANNGGANG-THNRRLSLNAN 537

Query: 262 QLGSNST 265
           Q GS ST
Sbjct: 538 QNGSRST 543

[Close]
BLAST of Carg20449 vs. ExPASy Swiss-Prot
Match: Q8LEG3 (65-kDa microtubule-associated protein 2 OS=Arabidopsis thaliana OX=3702 GN=MAP65-2 PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.2e-60
Identity = 144/287 (50.17%), Postives = 192/287 (66.90%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AE EV RLDQLK+S MKE+  KKQ ELE+I  ++H+EI   +  E+I++LI++G  +  
Sbjct: 298 QAEVEVDRLDQLKSSRMKEIAFKKQSELEEIYARAHIEIKPEVVRERIMSLIDAGNTEPT 357

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
            LL  MD QI++AKEEA SRK I+++VEKWM AC+EE WLE+Y+ DQ+RYS SRGAH NL
Sbjct: 358 ELLADMDSQIAKAKEEAFSRKEILDRVEKWMSACEEESWLEDYNRDQNRYSASRGAHLNL 417

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           +RAE+ARI V+KI A+V+ L+AKT++WE E S  F YD VPLLAML+EY  LRQE+E+EK
Sbjct: 418 KRAEKARILVSKITAMVDTLIAKTRAWEEENSMSFEYDGVPLLAMLDEYTMLRQEREDEK 477

Query: 202 MRLREKKKFQTQVAIEPESFYGSRPS----TSSRRLSNASINGGYSNAITLNRRLSLGIQ 261
            RL+E+KK Q Q   + ES +GS+PS     S+++     +NGG  N  T  RRLS+   
Sbjct: 478 RRLKEQKKQQEQPHTDQESAFGSKPSPARPVSAKKPVGTRVNGGGLNE-TPMRRLSMNSN 537

Query: 262 QLGSNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVS 305
           Q GS S        S  K    S         N+V+  +D+ AS VS
Sbjct: 538 QNGSKSKRD-----SLNKIASPS---------NIVANTKDDAASPVS 569

[Close]
BLAST of Carg20449 vs. ExPASy Swiss-Prot
Match: Q9FHM4 (65-kDa microtubule-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=MAP65-3 PE=1 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 2.0e-52
Identity = 125/252 (49.60%), Postives = 165/252 (65.48%), Query Frame = 0 

Query: 7   NELGANYVKKKKKNFKAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEM 66
           N L  +++K        EAEV RLD++KAS MKEL +KK+ ELE+IC K+H+   S   +
Sbjct: 290 NSLSEDFIK------YVEAEVVRLDEVKASKMKELVLKKRSELEEICRKTHLLPVSDSAI 349

Query: 67  EKIINLINSGEIDHVNLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSM 126
           ++ I  I SG +D   +L  +++ IS+ KEEA SRK I+E+VEKW+ ACDEE WLEEY+ 
Sbjct: 350 DQTIVAIESGIVDATMVLEHLEQHISKIKEEALSRKEILERVEKWLSACDEESWLEEYNR 409

Query: 127 DQSRYSVSRGAHKNLRRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAM 186
           D +RY+  RGAH  L+RAE+AR  V K+P +VE L +KT  WE E    FLYD + LL+M
Sbjct: 410 DDNRYNAGRGAHLTLKRAEKARNLVTKLPGMVEALASKTIVWEQENGIEFLYDGIRLLSM 469

Query: 187 LEEYNALRQEKEEEKMRLREKKKFQTQVAIEPESFYGSRPSTS---SRRLSNASINGGYS 246
           LEEYN LRQE+EEE  R R++KK Q Q+  E E+ YGS+PS S     + +     GG S
Sbjct: 470 LEEYNILRQEREEEHRRQRDQKKLQGQLIAEQEALYGSKPSPSKPLGGKKAPRMSTGGAS 529

Query: 247 NAITLNRRLSLG 256
                NRRLSLG
Sbjct: 530 -----NRRLSLG 530

[Close]
BLAST of Carg20449 vs. ExPASy Swiss-Prot
Match: Q9SIS3 (65-kDa microtubule-associated protein 6 OS=Arabidopsis thaliana OX=3702 GN=MAP65-6 PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.7e-49
Identity = 124/302 (41.06%), Postives = 186/302 (61.59%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +   EV  L +LKAS MKEL +K++ ELED+C  +H++  +    EK   LI+SG +D  
Sbjct: 308 QVSTEVDSLSKLKASRMKELVMKRRSELEDLCRLTHIQPDTSTSAEKSTALIDSGLVDPS 367

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
            LL +++ QI++ K+EA SRK IM+++++W+ AC+EE WLEEY++D++RYS  RG H NL
Sbjct: 368 ELLANIEMQINKIKDEAQSRKDIMDRIDRWLSACEEENWLEEYNLDENRYSAGRGGHVNL 427

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           +RAERAR+T+NKIP +V+ L+ KT  WE +  K FLYD V L+ +LE+Y   R+++EEEK
Sbjct: 428 KRAERARVTINKIPGMVDTLIKKTLVWEEDMQKSFLYDGVRLVNILEDYKLTRKQQEEEK 487

Query: 202 MRLREKKKFQTQVAIEPESFYGSRP----STSSRRLSNASINGGYSNAITLNRRLSLGIQ 261
            R R++KK Q  +  + ES YGS+P    S+S R+ +  +I+ G  +     RR S+G  
Sbjct: 488 KRYRDQKKRQDLLLTQRESIYGSKPSPRRSSSFRKPNGFNISNGNGSVPPTPRRGSVGTT 547

Query: 262 Q----LGSNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVST---FSGPI 313
                L   S +  +    + KE +      TPL  N V+  +++T S   T    S P 
Sbjct: 548 TPDVLLTPRSYSGHHRQNGYFKEVRRL--STTPL--NYVAMQKEDTVSTTYTSIYSSEPD 605

[Close]
BLAST of Carg20449 vs. ExPASy TrEMBL
Match: A0A6J1GHQ4 (65-kDa microtubule-associated protein 8-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454042 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.2e-150
Identity = 290/291 (99.66%), Postives = 291/291 (100.00%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV
Sbjct: 303 QAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 362

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
           NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL
Sbjct: 363 NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 422

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK
Sbjct: 423 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 482

Query: 202 MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS 261
           MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS
Sbjct: 483 MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS 542

Query: 262 NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 313
           NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP
Sbjct: 543 NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 593

[Close]
BLAST of Carg20449 vs. ExPASy TrEMBL
Match: A0A6J1GGQ7 (65-kDa microtubule-associated protein 8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454042 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 5.4e-149
Identity = 290/292 (99.32%), Postives = 291/292 (99.66%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV
Sbjct: 303 QAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 362

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
           NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL
Sbjct: 363 NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 422

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK
Sbjct: 423 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 482

Query: 202 MRLR-EKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLG 261
           MRLR EKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLG
Sbjct: 483 MRLRQEKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLG 542

Query: 262 SNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 313
           SNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP
Sbjct: 543 SNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 594

[Close]
BLAST of Carg20449 vs. ExPASy TrEMBL
Match: A0A6J1KQ37 (65-kDa microtubule-associated protein 8-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496583 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.2e-148
Identity = 287/291 (98.63%), Postives = 289/291 (99.31%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV
Sbjct: 303 QAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 362

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
           NLLTSMDEQI RAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL
Sbjct: 363 NLLTSMDEQILRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 422

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK
Sbjct: 423 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 482

Query: 202 MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS 261
           MRLREKKKFQTQVAIEPE+ YGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS
Sbjct: 483 MRLREKKKFQTQVAIEPENIYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS 542

Query: 262 NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 313
           NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP
Sbjct: 543 NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 593

[Close]
BLAST of Carg20449 vs. ExPASy TrEMBL
Match: A0A6J1KRL5 (65-kDa microtubule-associated protein 8-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496583 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.0e-147
Identity = 287/292 (98.29%), Postives = 289/292 (98.97%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV
Sbjct: 303 QAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 362

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
           NLLTSMDEQI RAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL
Sbjct: 363 NLLTSMDEQILRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 422

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK
Sbjct: 423 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 482

Query: 202 MRLR-EKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLG 261
           MRLR EKKKFQTQVAIEPE+ YGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLG
Sbjct: 483 MRLRQEKKKFQTQVAIEPENIYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLG 542

Query: 262 SNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 313
           SNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP
Sbjct: 543 SNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 594

[Close]
BLAST of Carg20449 vs. ExPASy TrEMBL
Match: A0A6J1BSB0 (65-kDa microtubule-associated protein 8 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005092 PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.2e-135
Identity = 266/291 (91.41%), Postives = 272/291 (93.47%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AEAEVKRLDQLKAS MKELFIKKQKELEDICNKSHMEIPSR EMEKIINLINSGEIDH 
Sbjct: 305 QAEAEVKRLDQLKASKMKELFIKKQKELEDICNKSHMEIPSRSEMEKIINLINSGEIDHA 364

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
           NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL
Sbjct: 365 NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 424

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           RRAERARITVNKIPALVELLL KT SWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK
Sbjct: 425 RRAERARITVNKIPALVELLLVKTTSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 484

Query: 202 MRLREKKKFQTQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLGS 261
            RLREKKK QTQV IEPE+FYG RPSTSSRRLSNASINGGYSNA+ LNRRLSLGIQQLGS
Sbjct: 485 RRLREKKKVQTQVVIEPENFYGPRPSTSSRRLSNASINGGYSNAMALNRRLSLGIQQLGS 544

Query: 262 NSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVSTFSGPISP 313
           NS NSA  G SF+KEGK SP Q+  LRH LVSQ+RDETASVVSTFSGPISP
Sbjct: 545 NSMNSATQGISFIKEGKVSPGQNMTLRHTLVSQVRDETASVVSTFSGPISP 595

[Close]
BLAST of Carg20449 vs. TAIR 10
Match: AT1G27920.1 (microtubule-associated protein 65-8 )

HSP 1 Score: 300.4 bits (768), Expect = 1.6e-81
Identity = 167/260 (64.23%), Postives = 204/260 (78.46%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AEAEVKRLDQLKAS +KELFIKKQKELED CN SHME PS  EM  I NL++SGE+DHV
Sbjct: 305 QAEAEVKRLDQLKASRIKELFIKKQKELEDTCNMSHMETPS-TEMGNITNLVDSGEVDHV 364

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
           +LL +MDE+I+RAKEEA+SRK I+EKV++WMLA DEERWLEEY  D++RYSVSR AH+NL
Sbjct: 365 DLLAAMDEKIARAKEEAASRKGIIEKVDRWMLASDEERWLEEYDQDENRYSVSRNAHRNL 424

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           RRAERARITV+KI  LVE +L K KSWE ER KVFLY+EVPL+AML+EYN LRQEKE EK
Sbjct: 425 RRAERARITVSKISGLVESILVKAKSWEVERQKVFLYNEVPLVAMLQEYNKLRQEKEMEK 484

Query: 202 MRLREKKKFQ-TQVAIEPESFYGSRPSTSSRRLSNASINGGYSNAITLNRRLSLGIQQLG 261
            RLRE KK    Q   E ++FY +RP++S+RR+SN S+NGG+ +   +NR+ S G     
Sbjct: 485 QRLREMKKMSIPQPVAEGDNFYMARPASSNRRISNRSMNGGFGSGSPINRKYSGGFNNTN 544

Query: 262 SNSTNSANVGTSFMKEGKTS 281
           +N T    +GTS  +E + S
Sbjct: 545 NNYT---ALGTSIRRESRKS 560

[Close]
BLAST of Carg20449 vs. TAIR 10
Match: AT5G55230.2 (microtubule-associated proteins 65-1 )

HSP 1 Score: 249.6 bits (636), Expect = 3.3e-66
Identity = 143/250 (57.20%), Postives = 181/250 (72.40%), Query Frame = 0 

Query: 19  KNFKAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEI 78
           K  KAE EV RLDQLKAS MKE+  KKQ ELE+I  ++H+E+      E+I++LI+SG +
Sbjct: 324 KFVKAEVEVDRLDQLKASRMKEIAFKKQSELEEIYARAHVEVNPESARERIMSLIDSGNV 383

Query: 79  DHVNLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAH 138
           +   LL  MD QIS+AKEEA SRK I+++VEKWM AC+EE WLE+Y+ DQ+RYS SRGAH
Sbjct: 384 EPTELLADMDSQISKAKEEAFSRKDILDRVEKWMSACEEESWLEDYNRDQNRYSASRGAH 443

Query: 139 KNLRRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKE 198
            NL+RAE+ARI V+KIPA+V+ L+AKT++WE E S  F YD VPLLAML+EY  LRQE+E
Sbjct: 444 LNLKRAEKARILVSKIPAMVDTLVAKTRAWEEEHSMSFAYDGVPLLAMLDEYGMLRQERE 503

Query: 199 EEKMRLREKKKFQTQVAIEPESFYGSRPS----TSSRRLSNASINGGYSNAITLNRRLSL 258
           EEK RLRE+KK Q Q  +E ES + +RPS     S+++      N G +N  T NRRLSL
Sbjct: 504 EEKRRLREQKKVQEQPHVEQESAFSTRPSPARPVSAKKTVGPRANNGGANG-THNRRLSL 563

Query: 259 GIQQLGSNST 265
              Q GS ST
Sbjct: 564 NANQNGSRST 572

[Close]
BLAST of Carg20449 vs. TAIR 10
Match: AT5G55230.1 (microtubule-associated proteins 65-1 )

HSP 1 Score: 248.1 bits (632), Expect = 9.6e-66
Identity = 141/247 (57.09%), Postives = 180/247 (72.87%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AE EV RLDQLKAS MKE+  KKQ ELE+I  ++H+E+      E+I++LI+SG ++  
Sbjct: 298 QAEVEVDRLDQLKASRMKEIAFKKQSELEEIYARAHVEVNPESARERIMSLIDSGNVEPT 357

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
            LL  MD QIS+AKEEA SRK I+++VEKWM AC+EE WLE+Y+ DQ+RYS SRGAH NL
Sbjct: 358 ELLADMDSQISKAKEEAFSRKDILDRVEKWMSACEEESWLEDYNRDQNRYSASRGAHLNL 417

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           +RAE+ARI V+KIPA+V+ L+AKT++WE E S  F YD VPLLAML+EY  LRQE+EEEK
Sbjct: 418 KRAEKARILVSKIPAMVDTLVAKTRAWEEEHSMSFAYDGVPLLAMLDEYGMLRQEREEEK 477

Query: 202 MRLREKKKFQTQVAIEPESFYGSRPS----TSSRRLSNASINGGYSNAITLNRRLSLGIQ 261
            RLRE+KK Q Q  +E ES + +RPS     S+++      N G +N  T NRRLSL   
Sbjct: 478 RRLREQKKVQEQPHVEQESAFSTRPSPARPVSAKKTVGPRANNGGANG-THNRRLSLNAN 537

Query: 262 QLGSNST 265
           Q GS ST
Sbjct: 538 QNGSRST 543

[Close]
BLAST of Carg20449 vs. TAIR 10
Match: AT4G26760.1 (microtubule-associated protein 65-2 )

HSP 1 Score: 235.0 bits (598), Expect = 8.4e-62
Identity = 144/287 (50.17%), Postives = 192/287 (66.90%), Query Frame = 0 

Query: 22  KAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEMEKIINLINSGEIDHV 81
           +AE EV RLDQLK+S MKE+  KKQ ELE+I  ++H+EI   +  E+I++LI++G  +  
Sbjct: 298 QAEVEVDRLDQLKSSRMKEIAFKKQSELEEIYARAHIEIKPEVVRERIMSLIDAGNTEPT 357

Query: 82  NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMDQSRYSVSRGAHKNL 141
            LL  MD QI++AKEEA SRK I+++VEKWM AC+EE WLE+Y+ DQ+RYS SRGAH NL
Sbjct: 358 ELLADMDSQIAKAKEEAFSRKEILDRVEKWMSACEEESWLEDYNRDQNRYSASRGAHLNL 417

Query: 142 RRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAMLEEYNALRQEKEEEK 201
           +RAE+ARI V+KI A+V+ L+AKT++WE E S  F YD VPLLAML+EY  LRQE+E+EK
Sbjct: 418 KRAEKARILVSKITAMVDTLIAKTRAWEEENSMSFEYDGVPLLAMLDEYTMLRQEREDEK 477

Query: 202 MRLREKKKFQTQVAIEPESFYGSRPS----TSSRRLSNASINGGYSNAITLNRRLSLGIQ 261
            RL+E+KK Q Q   + ES +GS+PS     S+++     +NGG  N  T  RRLS+   
Sbjct: 478 RRLKEQKKQQEQPHTDQESAFGSKPSPARPVSAKKPVGTRVNGGGLNE-TPMRRLSMNSN 537

Query: 262 QLGSNSTNSANVGTSFMKEGKTSPEQHTPLRHNLVSQIRDETASVVS 305
           Q GS S        S  K    S         N+V+  +D+ AS VS
Sbjct: 538 QNGSKSKRD-----SLNKIASPS---------NIVANTKDDAASPVS 569

[Close]
BLAST of Carg20449 vs. TAIR 10
Match: AT5G51600.1 (Microtubule associated protein (MAP65/ASE1) family protein )

HSP 1 Score: 207.6 bits (527), Expect = 1.4e-53
Identity = 125/252 (49.60%), Postives = 165/252 (65.48%), Query Frame = 0 

Query: 7   NELGANYVKKKKKNFKAEAEVKRLDQLKASMMKELFIKKQKELEDICNKSHMEIPSRLEM 66
           N L  +++K        EAEV RLD++KAS MKEL +KK+ ELE+IC K+H+   S   +
Sbjct: 290 NSLSEDFIK------YVEAEVVRLDEVKASKMKELVLKKRSELEEICRKTHLLPVSDSAI 349

Query: 67  EKIINLINSGEIDHVNLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSM 126
           ++ I  I SG +D   +L  +++ IS+ KEEA SRK I+E+VEKW+ ACDEE WLEEY+ 
Sbjct: 350 DQTIVAIESGIVDATMVLEHLEQHISKIKEEALSRKEILERVEKWLSACDEESWLEEYNR 409

Query: 127 DQSRYSVSRGAHKNLRRAERARITVNKIPALVELLLAKTKSWEAERSKVFLYDEVPLLAM 186
           D +RY+  RGAH  L+RAE+AR  V K+P +VE L +KT  WE E    FLYD + LL+M
Sbjct: 410 DDNRYNAGRGAHLTLKRAEKARNLVTKLPGMVEALASKTIVWEQENGIEFLYDGIRLLSM 469

Query: 187 LEEYNALRQEKEEEKMRLREKKKFQTQVAIEPESFYGSRPSTS---SRRLSNASINGGYS 246
           LEEYN LRQE+EEE  R R++KK Q Q+  E E+ YGS+PS S     + +     GG S
Sbjct: 470 LEEYNILRQEREEEHRRQRDQKKLQGQLIAEQEALYGSKPSPSKPLGGKKAPRMSTGGAS 529

Query: 247 NAITLNRRLSLG 256
                NRRLSLG
Sbjct: 530 -----NRRLSLG 530

The following BLAST results are available for this feature:
BLAST of Carg20449 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7020141.11.8e-162100.0065-kDa microtubule-associated protein 8, partial [Cucurbita argyrosperma subsp. ... [more]
KAG6585221.14.5e-15099.6665-kDa microtubule-associated protein 8, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022951089.14.5e-15099.6665-kDa microtubule-associated protein 8-like isoform X2 [Cucurbita moschata][more]
XP_023537211.12.2e-14999.3165-kDa microtubule-associated protein 8-like isoform X2 [Cucurbita pepo subsp. p... [more]
XP_022951088.11.1e-14899.3265-kDa microtubule-associated protein 8-like isoform X1 [Cucurbita moschata][more]
BLAST of Carg20449 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9C7G02.3e-8064.2365-kDa microtubule-associated protein 8 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
Q9FLP01.4e-6457.0965-kDa microtubule-associated protein 1 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
Q8LEG31.2e-6050.1765-kDa microtubule-associated protein 2 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
Q9FHM42.0e-5249.6065-kDa microtubule-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
Q9SIS34.7e-4941.0665-kDa microtubule-associated protein 6 OS=Arabidopsis thaliana OX=3702 GN=MAP65... [more]
BLAST of Carg20449 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GHQ42.2e-15099.6665-kDa microtubule-associated protein 8-like isoform X2 OS=Cucurbita moschata OX... [more]
A0A6J1GGQ75.4e-14999.3265-kDa microtubule-associated protein 8-like isoform X1 OS=Cucurbita moschata OX... [more]
A0A6J1KQ371.2e-14898.6365-kDa microtubule-associated protein 8-like isoform X2 OS=Cucurbita maxima OX=3... [more]
A0A6J1KRL53.0e-14798.2965-kDa microtubule-associated protein 8-like isoform X1 OS=Cucurbita maxima OX=3... [more]
A0A6J1BSB01.2e-13591.4165-kDa microtubule-associated protein 8 isoform X1 OS=Momordica charantia OX=367... [more]
BLAST of Carg20449 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G27920.11.6e-8164.23microtubule-associated protein 65-8 [more]
AT5G55230.23.3e-6657.20microtubule-associated proteins 65-1 [more]
AT5G55230.19.6e-6657.09microtubule-associated proteins 65-1 [more]
AT4G26760.18.4e-6250.17microtubule-associated protein 65-2 [more]
AT5G51600.11.4e-5349.60Microtubule associated protein (MAP65/ASE1) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 183..214
NoneNo IPR availablePFAMPF03999MAP65_ASE1coord: 23..236
e-value: 5.9E-40
score: 137.5
NoneNo IPR availableGENE3D1.20.58.1520coord: 91..224
e-value: 5.9E-36
score: 125.4
NoneNo IPR availablePANTHERPTHR19321:SF365-KDA MICROTUBULE-ASSOCIATED PROTEIN 8coord: 22..312
IPR007145Microtubule-associated protein, MAP65/Ase1/PRC1PANTHERPTHR19321PROTEIN REGULATOR OF CYTOKINESIS 1 PRC1-RELATEDcoord: 22..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20449-RACarg20449-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000226 microtubule cytoskeleton organization
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005874 microtubule
cellular_component GO:0005819 spindle
molecular_function GO:0008017 microtubule binding