Carg24675 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg24675 vs. NCBI nr
Match: KAG7022276.1 (V-type proton ATPase subunit a3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1545.8 bits (4001), Expect = 0.0e+00
Identity = 789/789 (100.00%), Postives = 789/789 (100.00%), Query Frame = 0

Query: 1   MPITLGSSITPDHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSS 60
           MPITLGSSITPDHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSS
Sbjct: 1   MPITLGSSITPDHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSS 60

Query: 61  KSSVSQVDISIDDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQ 120
           KSSVSQVDISIDDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQ
Sbjct: 61  KSSVSQVDISIDDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQ 120

Query: 121 SSAVEQQREFESRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILF 180
           SSAVEQQREFESRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILF
Sbjct: 121 SSAVEQQREFESRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILF 180

Query: 181 RATRGNVFLKQAAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFT 240
           RATRGNVFLKQAAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFT
Sbjct: 181 RATRGNVFLKQAAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFT 240

Query: 241 EDLGKQAQMIAEVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILN 300
           EDLGKQAQMIAEVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILN
Sbjct: 241 EDLGKQAQMIAEVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILN 300

Query: 301 MLSLDVTKKCLVAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTN 360
           MLSLDVTKKCLVAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTN
Sbjct: 301 MLSLDVTKKCLVAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTN 360

Query: 361 KFSTAFQEIVDAYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREK 420
           KFSTAFQEIVDAYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREK
Sbjct: 361 KFSTAFQEIVDAYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREK 420

Query: 421 KLSSQKLGDITEMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSD 480
           KLSSQKLGDITEMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSD
Sbjct: 421 KLSSQKLGDITEMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSD 480

Query: 481 STTVGLIKAGSTYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATF 540
           STTVGLIKAGSTYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATF
Sbjct: 481 STTVGLIKAGSTYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATF 540

Query: 541 FRNSINIWFQFLPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQ 600
           FRNSINIWFQFLPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQ
Sbjct: 541 FRNSINIWFQFLPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQ 600

Query: 601 LFPGQKNVQIVLLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHH 660
           LFPGQKNVQIVLLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHH
Sbjct: 601 LFPGQKNVQIVLLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHH 660

Query: 661 DSHGHEEFEFSEVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLS 720
           DSHGHEEFEFSEVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLS
Sbjct: 661 DSHGHEEFEFSEVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLS 720

Query: 721 AGFNNVIILIVGIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFS 780
           AGFNNVIILIVGIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFS
Sbjct: 721 AGFNNVIILIVGIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFS 780

Query: 781 FALLDEDDE 790
           FALLDEDDE
Sbjct: 781 FALLDEDDE 789

BLAST of Carg24675 vs. NCBI nr
Match: XP_023530527.1 (V-type proton ATPase subunit a3-like [Cucurbita pepo subsp. pepo] >KAG6588428.1 V-type proton ATPase subunit a3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1503.8 bits (3892), Expect = 0.0e+00
Identity = 771/778 (99.10%), Postives = 772/778 (99.23%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 71
           D  +  F  LNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI
Sbjct: 41  DLGLLQFKDLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 100

Query: 72  DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 131
           DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE
Sbjct: 101 DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 160

Query: 132 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 191
           SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ
Sbjct: 161 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 220

Query: 192 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 251
           AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA
Sbjct: 221 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 280

Query: 252 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 311
           EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL
Sbjct: 281 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 340

Query: 312 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 371
           VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD
Sbjct: 341 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 400

Query: 372 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 431
           AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT
Sbjct: 401 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 460

Query: 432 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 491
           EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS
Sbjct: 461 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 520

Query: 492 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 551
           TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF
Sbjct: 521 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 580

Query: 552 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 611
           LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV
Sbjct: 581 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 640

Query: 612 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 671
           LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS
Sbjct: 641 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 700

Query: 672 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 731
           EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV
Sbjct: 701 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 760

Query: 732 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE
Sbjct: 761 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 818

BLAST of Carg24675 vs. NCBI nr
Match: XP_022932008.1 (V-type proton ATPase subunit a3-like [Cucurbita moschata])

HSP 1 Score: 1502.6 bits (3889), Expect = 0.0e+00
Identity = 770/778 (98.97%), Postives = 772/778 (99.23%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 71
           D  +  F  LNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI
Sbjct: 41  DLGLLQFKDLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 100

Query: 72  DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 131
           DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE
Sbjct: 101 DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 160

Query: 132 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 191
           SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ
Sbjct: 161 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 220

Query: 192 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 251
           AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA
Sbjct: 221 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 280

Query: 252 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 311
           EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL
Sbjct: 281 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 340

Query: 312 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 371
           VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD
Sbjct: 341 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 400

Query: 372 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 431
           AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT
Sbjct: 401 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 460

Query: 432 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 491
           EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS
Sbjct: 461 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 520

Query: 492 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 551
           TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF
Sbjct: 521 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 580

Query: 552 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 611
           LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV
Sbjct: 581 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 640

Query: 612 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 671
           LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLE+DSHHDSHGHEEFEFS
Sbjct: 641 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLEVDSHHDSHGHEEFEFS 700

Query: 672 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 731
           EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV
Sbjct: 701 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 760

Query: 732 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE
Sbjct: 761 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 818

BLAST of Carg24675 vs. NCBI nr
Match: XP_022970565.1 (V-type proton ATPase subunit a3-like [Cucurbita maxima])

HSP 1 Score: 1494.2 bits (3867), Expect = 0.0e+00
Identity = 765/778 (98.33%), Postives = 771/778 (99.10%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 71
           D  +  F  LNVEKSPFQRTYAAQIKRCGEMARKL+FFKEQMLKAGLSSKSSVSQVDISI
Sbjct: 41  DLGLLQFKDLNVEKSPFQRTYAAQIKRCGEMARKLNFFKEQMLKAGLSSKSSVSQVDISI 100

Query: 72  DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 131
           DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVL KAGEFFS+AQSSAVEQQREFE
Sbjct: 101 DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLHKAGEFFSAAQSSAVEQQREFE 160

Query: 132 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 191
           SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ
Sbjct: 161 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 220

Query: 192 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 251
           AAV+DPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMI+
Sbjct: 221 AAVEDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIS 280

Query: 252 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 311
           EVSGKLAELKTTID+GLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL
Sbjct: 281 EVSGKLAELKTTIDLGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 340

Query: 312 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 371
           VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD
Sbjct: 341 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 400

Query: 372 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 431
           AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT
Sbjct: 401 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 460

Query: 432 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 491
           EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS
Sbjct: 461 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 520

Query: 492 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 551
           TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF
Sbjct: 521 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 580

Query: 552 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 611
           LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV
Sbjct: 581 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 640

Query: 612 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 671
           LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS
Sbjct: 641 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 700

Query: 672 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 731
           EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV
Sbjct: 701 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 760

Query: 732 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE
Sbjct: 761 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 818

BLAST of Carg24675 vs. NCBI nr
Match: XP_038888026.1 (V-type proton ATPase subunit a3-like [Benincasa hispida])

HSP 1 Score: 1454.1 bits (3763), Expect = 0.0e+00
Identity = 736/778 (94.60%), Postives = 761/778 (97.81%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 71
           D  +  F  LN +KSPFQRTYAAQIKRCGEMARKL+FFKEQMLKAGLSSKSSVS+VDI+I
Sbjct: 41  DLGLLQFKDLNADKSPFQRTYAAQIKRCGEMARKLNFFKEQMLKAGLSSKSSVSEVDINI 100

Query: 72  DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 131
           DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLV+QKAGEFFS+AQSSAVEQQREFE
Sbjct: 101 DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVMQKAGEFFSAAQSSAVEQQREFE 160

Query: 132 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 191
           SRR GGDSIEVPLLLEQES  DQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ
Sbjct: 161 SRRTGGDSIEVPLLLEQESFVDQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 220

Query: 192 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 251
           AAV+DPV DPISGEKVEKNVF+VFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMI 
Sbjct: 221 AAVEDPVADPISGEKVEKNVFIVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIT 280

Query: 252 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 311
           EVSGKL+ELKTTID+GLLHRGNLL+ IGEHFENWN++ARKEK+IYHILNMLSLDVTKKCL
Sbjct: 281 EVSGKLSELKTTIDIGLLHRGNLLQTIGEHFENWNLLARKEKAIYHILNMLSLDVTKKCL 340

Query: 312 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 371
           VAEGWGPVFATKQIQDALQRAA+DSNSQVGPIFQVLLTTEAPPTYFRTNKFS+AFQEIVD
Sbjct: 341 VAEGWGPVFATKQIQDALQRAASDSNSQVGPIFQVLLTTEAPPTYFRTNKFSSAFQEIVD 400

Query: 372 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 431
           AYGVA+YQEANPGVYTIVTFPFLFAVMFGDWGHGICL LATLYFILREKKLSSQKLGDIT
Sbjct: 401 AYGVARYQEANPGVYTIVTFPFLFAVMFGDWGHGICLFLATLYFILREKKLSSQKLGDIT 460

Query: 432 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 491
           EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAY CRSPDCSDSTT+GLIK GS
Sbjct: 461 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYACRSPDCSDSTTMGLIKVGS 520

Query: 492 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 551
           TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF
Sbjct: 521 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 580

Query: 552 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 611
           LPQMIFLNSLFGYLSLLIIIKWCTGS ADLYHVMIYMFLGPT+DLAENQLFPGQKNVQIV
Sbjct: 581 LPQMIFLNSLFGYLSLLIIIKWCTGSNADLYHVMIYMFLGPTDDLAENQLFPGQKNVQIV 640

Query: 612 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 671
           LLLLAL+AVPWMLLPKPFLLK+QHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS
Sbjct: 641 LLLLALVAVPWMLLPKPFLLKRQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 700

Query: 672 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 731
           EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNN+IILIV
Sbjct: 701 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNIIILIV 760

Query: 732 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKF+PFSFALL+EDDE
Sbjct: 761 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFHPFSFALLNEDDE 818

BLAST of Carg24675 vs. ExPASy Swiss-Prot
Match: Q8W4S4 (V-type proton ATPase subunit a3 OS=Arabidopsis thaliana OX=3702 GN=VHA-a3 PE=1 SV=1)

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 605/780 (77.56%), Postives = 689/780 (88.33%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKS-SVSQVDIS 71
           D  +  F  LN EKSPFQRTYAAQIKRCGEMARK+ FF++QM KAG+ +K     + DI 
Sbjct: 43  DLGLVQFKDLNSEKSPFQRTYAAQIKRCGEMARKIRFFRDQMSKAGVPAKEMQGKENDID 102

Query: 72  IDDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREF 131
           +DD+EVKLGELEAELVEINAN++KLQRSYNEL+EYKLVLQKAGEFFSSA  SA +QQRE 
Sbjct: 103 LDDVEVKLGELEAELVEINANNDKLQRSYNELMEYKLVLQKAGEFFSSAHRSAADQQRET 162

Query: 132 ESRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLK 191
           ES++ G D +E P LL++E S D +K V LGFL+GLVPREKSM FERILFRATRGN+F++
Sbjct: 163 ESQQAGEDLLESP-LLQEEKSIDSTKQVKLGFLTGLVPREKSMVFERILFRATRGNIFIR 222

Query: 192 QAAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMI 251
           Q  +++PV DP SGEK EKNVFVVFYSGERAK+KILKICEAFGANRYPF+EDLG+QAQMI
Sbjct: 223 QTVIEEPVIDPNSGEKAEKNVFVVFYSGERAKSKILKICEAFGANRYPFSEDLGRQAQMI 282

Query: 252 AEVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKC 311
            EVSG+L+ELKTTID GL  R  LL+ IG+ FE WN+  RKEK+IYH LNMLSLDVTKKC
Sbjct: 283 TEVSGRLSELKTTIDAGLGQRNILLQTIGDKFELWNLKVRKEKAIYHTLNMLSLDVTKKC 342

Query: 312 LVAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIV 371
           LVAEGW PVFA+++IQDALQRAA DSNSQVG IFQVL T E+PPTYFRTNKF++A QEIV
Sbjct: 343 LVAEGWSPVFASREIQDALQRAAVDSNSQVGSIFQVLRTKESPPTYFRTNKFTSAIQEIV 402

Query: 372 DAYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDI 431
           DAYGVAKYQEANPGV+TIVTFPFLFAVMFGDWGHGIC+LLAT+Y IL+EKKL+SQKLGDI
Sbjct: 403 DAYGVAKYQEANPGVFTIVTFPFLFAVMFGDWGHGICILLATMYLILKEKKLASQKLGDI 462

Query: 432 TEMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAG 491
            EMAFGGRYVILMMSLFSIYTGLIYNEFFS+PF LF  SAY+CR   CS++TT+GLIK  
Sbjct: 463 MEMAFGGRYVILMMSLFSIYTGLIYNEFFSIPFPLFAPSAYDCRDVSCSEATTIGLIKVR 522

Query: 492 STYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQ 551
            TYPFGLDPVWHG+RSELPFLNSLKMKMSILLGV+QMNLGII+SYFNA FF++S+NIWFQ
Sbjct: 523 DTYPFGLDPVWHGSRSELPFLNSLKMKMSILLGVSQMNLGIIMSYFNARFFKSSVNIWFQ 582

Query: 552 FLPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQI 611
           F+PQMIFLNSLFGYLS+LIIIKWCTGS+ADLYHVMIYMFL P ++L ENQLFP QK +Q+
Sbjct: 583 FIPQMIFLNSLFGYLSVLIIIKWCTGSQADLYHVMIYMFLSPMDELGENQLFPHQKTLQL 642

Query: 612 VLLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSH-HDSHGHEEFE 671
           VLL LAL++VP MLLPKPF+LKKQHE R QGQ+YAPL   D+SL ++++   SHGHEEFE
Sbjct: 643 VLLFLALVSVPCMLLPKPFILKKQHEARHQGQAYAPLDETDESLHVETNGGGSHGHEEFE 702

Query: 672 FSEVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIIL 731
           FSE+FVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFY+KVL+L+ G+NN +IL
Sbjct: 703 FSEIFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYEKVLLLAWGYNNPLIL 762

Query: 732 IVGIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           IVG++VFIFATVGVLL+METLSAFLHALRLHWVEFQNKFYEGDGYKF PF+F     +DE
Sbjct: 763 IVGVLVFIFATVGVLLVMETLSAFLHALRLHWVEFQNKFYEGDGYKFAPFTFIFTANEDE 821

BLAST of Carg24675 vs. ExPASy Swiss-Prot
Match: Q9SJT7 (V-type proton ATPase subunit a2 OS=Arabidopsis thaliana OX=3702 GN=VHA-a2 PE=1 SV=1)

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 598/779 (76.77%), Postives = 678/779 (87.03%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVS-QVDIS 71
           D  +  F  LN EKSPFQRTYAAQIKRCGEMARK+ FFKEQM KAG++ K ++  + DI 
Sbjct: 44  DLGLVQFKDLNSEKSPFQRTYAAQIKRCGEMARKIRFFKEQMSKAGVTPKETLDRENDID 103

Query: 72  IDDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREF 131
           +DD+EVKL ELEAELVEINAN++KLQRSYNELVEYKLVL+KAGEFF+SA  SA  QQ E 
Sbjct: 104 LDDVEVKLEELEAELVEINANNDKLQRSYNELVEYKLVLEKAGEFFASAHRSATAQQSEI 163

Query: 132 ESRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLK 191
           E+ +VG D +E P LL++E S D +K V LGFL+GLVPREKSM FERILFRATRGN+F++
Sbjct: 164 ETEQVGEDLLEAP-LLQEEKSVDPTKQVKLGFLTGLVPREKSMVFERILFRATRGNIFIR 223

Query: 192 QAAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMI 251
           Q+ +++ V DP SGEK EKNVFVVFYSGERAK+KILKICEAFGANRYPF+EDLGKQAQM+
Sbjct: 224 QSVIEESVVDPNSGEKAEKNVFVVFYSGERAKSKILKICEAFGANRYPFSEDLGKQAQMM 283

Query: 252 AEVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKC 311
            EVSG+L+ELKTTI  GL  R  LL+ IG+ FE WN+  RKEK+IYH LNMLSLDVTKKC
Sbjct: 284 TEVSGRLSELKTTIGAGLDQRNILLETIGDKFEQWNLKIRKEKAIYHTLNMLSLDVTKKC 343

Query: 312 LVAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIV 371
           LV EGW PVFA  +IQDAL RAA DSNSQVG IFQVL T E PPT+FRTNKF+TAFQEIV
Sbjct: 344 LVGEGWSPVFAATEIQDALHRAAVDSNSQVGSIFQVLRTKEMPPTFFRTNKFTTAFQEIV 403

Query: 372 DAYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDI 431
           DAYGVAKYQEANP V+TIVTFPFLFAVMFGDWGHGICLLLAT+Y ILREKKLSSQKLGDI
Sbjct: 404 DAYGVAKYQEANPSVFTIVTFPFLFAVMFGDWGHGICLLLATMYLILREKKLSSQKLGDI 463

Query: 432 TEMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAG 491
            EMAFGGRYVI MMSLFSIYTGLIYNEFFS+P+ LF  SAY+CR   CS++TT+GLIK  
Sbjct: 464 MEMAFGGRYVIFMMSLFSIYTGLIYNEFFSIPYPLFASSAYDCRDVSCSEATTIGLIKTR 523

Query: 492 STYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQ 551
            TYPFG+DPVWHGTRSELPFLNSLKMKMSIL+GVAQMNLGII+S+FNA FF++++NIWFQ
Sbjct: 524 DTYPFGVDPVWHGTRSELPFLNSLKMKMSILIGVAQMNLGIIMSFFNAKFFKSAVNIWFQ 583

Query: 552 FLPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQI 611
           F+PQMIFLN LFGYLS+LIIIKWCTGS+ADLYHVMIYMFL P +DL ENQLFP QK VQ+
Sbjct: 584 FVPQMIFLNCLFGYLSVLIIIKWCTGSQADLYHVMIYMFLSPMDDLGENQLFPNQKIVQL 643

Query: 612 VLLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEF 671
             L LAL++VPWMLLPKPF+LKKQHE R QG SYA L   D+SL+++++   HGHEEFEF
Sbjct: 644 TFLFLALVSVPWMLLPKPFILKKQHEARHQGLSYAQLDETDESLQVETNGGGHGHEEFEF 703

Query: 672 SEVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILI 731
           SE+FVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFY+KVL+++ GFNNV I I
Sbjct: 704 SEIFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYEKVLLMAWGFNNVFIWI 763

Query: 732 VGIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           VGI+VFIFATVGVLL+METLSAFLHALRLHWVE+QNKFYEGDGYKF PF+F L+  +DE
Sbjct: 764 VGILVFIFATVGVLLVMETLSAFLHALRLHWVEYQNKFYEGDGYKFAPFTFTLVGNEDE 821

BLAST of Carg24675 vs. ExPASy Swiss-Prot
Match: Q8RWZ7 (V-type proton ATPase subunit a1 OS=Arabidopsis thaliana OX=3702 GN=VHA-a1 PE=2 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 1.4e-280
Identity = 485/772 (62.82%), Postives = 604/772 (78.24%), Query Frame = 0

Query: 18  FFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVS-QVDISIDDLEV 77
           F  LN +KSPFQRT+A Q+KRCGEM+RKL FFK+Q+ KAGL     +  + DI++ DLE 
Sbjct: 46  FRDLNADKSPFQRTFANQVKRCGEMSRKLRFFKDQIDKAGLRCSPRLEIEPDIALGDLER 105

Query: 78  KLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREF-ESRRV 137
           +L + E E++E+N+NSEKL+++YNEL+E+K+VL+KA  F  S+ + A+ ++ E  ES   
Sbjct: 106 QLADHEHEVLEMNSNSEKLRQTYNELLEFKIVLEKASGFLVSSNTHAIGEEIELHESTYS 165

Query: 138 GGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQAAVD 197
               IE   LLEQE +   S    L F+SG++ ++K + FER+LFRATRGN+   Q   D
Sbjct: 166 NNGFIETASLLEQEMNPGHSNQSGLRFISGIINKDKLLKFERMLFRATRGNMLFNQTTSD 225

Query: 198 DPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIAEVSG 257
           + + DP + E VEK VFVVF+SGE+A+ KILKICEAFGAN YP  ED  KQ Q+  EV  
Sbjct: 226 EEIMDPSTSEMVEKVVFVVFFSGEQARTKILKICEAFGANCYPVPEDTTKQRQLTREVLS 285

Query: 258 KLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCLVAEG 317
           +L++L+ T+D G  HR N L ++G    NW    R+EK++Y  LNML+ DVTKKCLV EG
Sbjct: 286 RLSDLEATLDAGTRHRNNALNSVGYSLTNWITTVRREKAVYDTLNMLNFDVTKKCLVGEG 345

Query: 318 WGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVDAYGV 377
           W P FA  QI + LQRA  DS+SQVG IF V+   E+PPTYFRTNK + AFQEI+DAYGV
Sbjct: 346 WCPTFAKTQIHEVLQRATFDSSSQVGVIFHVMQAVESPPTYFRTNKLTNAFQEIIDAYGV 405

Query: 378 AKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDITEMAF 437
           A+YQEANP VY++VT+PFLFAVMFGDWGHG+CLLL  LY + RE+KLS+QKLG   EM F
Sbjct: 406 ARYQEANPAVYSVVTYPFLFAVMFGDWGHGLCLLLGALYLLARERKLSTQKLGSFMEMLF 465

Query: 438 GGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGSTYPF 497
           GGRYVIL+M+LFSIY GLIYNEFFSVPF +FG SAY+CR   CSD+ TVGLIK    YPF
Sbjct: 466 GGRYVILLMALFSIYCGLIYNEFFSVPFHIFGGSAYKCRDTTCSDAYTVGLIKYRDPYPF 525

Query: 498 GLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQFLPQM 557
           G+DP W G+R+ELP+LNSLKMKMSILLG+AQMNLG+I+S+FNA FF +S++I +QF+PQM
Sbjct: 526 GVDPSWRGSRTELPYLNSLKMKMSILLGIAQMNLGLILSFFNARFFGSSLDIRYQFIPQM 585

Query: 558 IFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIVLLLL 617
           IFLNSLFGYLSLLIIIKWCTGS+ADLYHVMIYMFL PTE+L EN+LF GQ+ +QIVLLLL
Sbjct: 586 IFLNSLFGYLSLLIIIKWCTGSQADLYHVMIYMFLSPTEELGENELFWGQRPLQIVLLLL 645

Query: 618 ALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELD---SHHDSHGHEEFEFSE 677
           A IAVPWML PKPF L+K H +RFQG++Y  L S++  L+++   +    H  EEF FSE
Sbjct: 646 AFIAVPWMLFPKPFALRKIHMERFQGRTYGVLVSSEVDLDVEPDSARGGGHHEEEFNFSE 705

Query: 678 VFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIVG 737
           +FVHQLIH+IEFVLG+VSNTASYLRLWALSLAHSELS+VFY+KVL+L+ G+ N++I ++G
Sbjct: 706 IFVHQLIHSIEFVLGSVSNTASYLRLWALSLAHSELSTVFYEKVLLLAWGYENILIRLIG 765

Query: 738 IIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALL 785
           + VF FAT  +LL+METLSAFLHALRLHWVEF  KF+ GDGYKF PFSFAL+
Sbjct: 766 VAVFAFATAFILLMMETLSAFLHALRLHWVEFMGKFFNGDGYKFKPFSFALI 817

BLAST of Carg24675 vs. ExPASy Swiss-Prot
Match: Q54E04 (Vacuolar proton translocating ATPase 100 kDa subunit OS=Dictyostelium discoideum OX=44689 GN=vatM PE=1 SV=2)

HSP 1 Score: 614.0 bits (1582), Expect = 2.4e-174
Identity = 361/797 (45.29%), Postives = 497/797 (62.36%), Query Frame = 0

Query: 18  FFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSK------SSVSQVDISI 77
           F   N   + FQR +  ++KRC +M +KL FF++Q+ K     K       SV   D  +
Sbjct: 41  FLDDNEHVNLFQRNFVNEVKRCDDMEKKLKFFEDQVKKEPKLQKLLPDNMLSVVDDDSQM 100

Query: 78  DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 137
           D+LE +  ELE+EL ++NAN E LQR+YNEL++ + VL K   FF    +    +  E  
Sbjct: 101 DELEGRFDELESELKQVNANQETLQRNYNELIQLRHVLTKDSVFFQENPNLIEGEGHEHS 160

Query: 138 SRRVGGDSIEVPLLLEQESSADQSKP-VNLGFLSGLVPREKSMAFERILFRATRGNVFLK 197
           +R         PLL E +  ++ +K  V LGF++G++  +K   F+R L+R TRGN ++K
Sbjct: 161 ARS--------PLLAEDQHVSEVAKQGVKLGFITGVMNTDKMPQFQRSLWRTTRGNNYVK 220

Query: 198 QAAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMI 257
            A +++ + DP +GE+  K VF+VF+ GER + KI KICE+FGAN Y   ++  +++ ++
Sbjct: 221 DARIEEEIIDPQTGEETAKTVFIVFFQGERLQQKIKKICESFGANIYDCPDNSFERSNLL 280

Query: 258 AEVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKC 317
            +V+ ++ +L   +     H+   L  I     +W      EKSIYH +N+   DV +KC
Sbjct: 281 QKVTVRITDLYEVLQRSKDHKRQTLAGIVPRLYSWKKKVLLEKSIYHTMNLFDYDVGRKC 340

Query: 318 LVAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIV 377
           L+A+GW P    ++IQ AL+ A T S + V  +  ++ T  +PPT+F TNK++++FQEIV
Sbjct: 341 LIAKGWTPKDKIEEIQLALRTATTRSGALVPSVLSIIKTEGSPPTHFETNKYTSSFQEIV 400

Query: 378 DAYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDI 437
           +AYG+A Y+E NP V TIVTFPFLF VMFGD GHG  LLL+ L  I  EKKL+ +KL ++
Sbjct: 401 NAYGIAHYREVNPAVLTIVTFPFLFGVMFGDVGHGALLLLSALGLISLEKKLAGKKLNEL 460

Query: 438 TEMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAG 497
            +M F GRYV+ +MSLFSIY G IYNE FS+P  +FG S Y   S       T GL    
Sbjct: 461 IQMPFDGRYVLFLMSLFSIYVGFIYNECFSIPMNIFG-SQYNLNS-------TTGLYTYQ 520

Query: 498 ST---YPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGI---IISYFNATFFRNS 557
            T   YP G+DP+W G  +EL + NS KMK+SI+ GV QM++GI   +++Y N       
Sbjct: 521 HTDRVYPVGVDPLWKGAPNELVYYNSFKMKLSIIFGVVQMSVGICFSLLNYLNQKGPIKI 580

Query: 558 INIWFQFLPQMIFLNSLFGYLSLLIIIKWCTG------SKAD---LYHVMIYMFLGP--T 617
           +NI  QF+PQMIFL S+FGY+S+LII+KW          K D   +   +I MFL P  T
Sbjct: 581 VNILTQFVPQMIFLWSIFGYMSVLIILKWVVPYRSFEVDKVDPPFILPTIIAMFLSPGGT 640

Query: 618 EDLAENQLFPGQKNVQIVLLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDS 677
            D+     F GQ  VQ  LL LALI++P ML+ KP  +K+ H   FQ      L   ++ 
Sbjct: 641 PDVV---FFSGQGAVQTALLFLALISIPVMLVIKPLFMKRFH---FQEVERKKLGHHEEE 700

Query: 678 LELDSHHDSHGHEEFEFSEVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFY 737
            + ++ +  H  EEFE  EVFVHQ+IHTIEFVLGAVSNTASYLRLWALSLAHSELSSVF+
Sbjct: 701 HDDEALYTGHHGEEFEMGEVFVHQVIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFW 760

Query: 738 DKVLVLSAGFNNVIILIVGIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDG 790
           +++L+      N  +  VG   ++ A+V VLLLME+LSAFLHALRLHWVEFQNKFY GDG
Sbjct: 761 ERILIGQVERGNPFLAFVGFGAWLGASVAVLLLMESLSAFLHALRLHWVEFQNKFYIGDG 815

BLAST of Carg24675 vs. ExPASy Swiss-Prot
Match: Q9HBG4 (V-type proton ATPase 116 kDa subunit a isoform 4 OS=Homo sapiens OX=9606 GN=ATP6V0A4 PE=1 SV=2)

HSP 1 Score: 604.0 bits (1556), Expect = 2.5e-171
Identity = 347/808 (42.95%), Postives = 492/808 (60.89%), Query Frame = 0

Query: 18  FFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQM---LKAGLSSKSSVSQVDISIDDL 77
           F  LN+  + FQR +  +++RC  + R L F +++M   +   L  KS ++ +   +  L
Sbjct: 37  FKDLNMNVNSFQRKFVNEVRRCESLERILRFLEDEMQNEIVVQLLEKSPLTPLPREMITL 96

Query: 78  EVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFESRR 137
           E  L +LE EL E N N + L++S+ EL E K +L+K  +FF +  + A     +F +  
Sbjct: 97  ETVLEKLEGELQEANQNQQALKQSFLELTELKYLLKKTQDFFETETNLA----DDFFTED 156

Query: 138 VGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQAAV 197
             G       LLE + +        LGF++G++ RE+  +FER+L+R  RGNV+LK + +
Sbjct: 157 TSG-------LLELK-AVPAYMTGKLGFIAGVINRERMASFERLLWRICRGNVYLKFSEM 216

Query: 198 DDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIAEVS 257
           D P+ DP++ E+++KN+F++FY GE+ + KI KIC+ F A  YP  E   ++ +M+  V+
Sbjct: 217 DAPLEDPVTKEEIQKNIFIIFYQGEQLRQKIKKICDGFRATVYPCPEPAVERREMLESVN 276

Query: 258 GKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCLVAE 317
            +L +L T I     HR  LL+    ++ +W I  +K K++YHILNM ++DVT++C++AE
Sbjct: 277 VRLEDLITVITQTESHRQRLLQEAAANWHSWLIKVQKMKAVYHILNMCNIDVTQQCVIAE 336

Query: 318 GWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVDAYG 377
            W PV    +I+ AL++    S S + PI   + +  APPT+ RTNKF+  FQ IVDAYG
Sbjct: 337 IWFPVADATRIKRALEQGMELSGSSMAPIMTTVQSKTAPPTFNRTNKFTAGFQNIVDAYG 396

Query: 378 VAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKL-GDITEM 437
           V  Y+E NP  YTI+TFPFLFAVMFGD GHG  +LLA L+ IL E++L SQK   +I   
Sbjct: 397 VGSYREINPAPYTIITFPFLFAVMFGDCGHGTVMLLAALWMILNERRLLSQKTDNEIWNT 456

Query: 438 AFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRS----------AYECRSPDCS---- 497
            F GRY+IL+M +FSIYTGLIYN+ FS    +FG S           +     + S    
Sbjct: 457 FFHGRYLILLMGIFSIYTGLIYNDCFSKSLNIFGSSWSVQPMFRNGTWNTHVMEESLYLQ 516

Query: 498 -DSTTVGLIKAGSTYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNA 557
            D    G +  G+ YPFG+DP+W+   ++L FLNS KMKMS++LG+ QM  G+I+S FN 
Sbjct: 517 LDPAIPG-VYFGNPYPFGIDPIWNLASNKLTFLNSYKMKMSVILGIVQMVFGVILSLFNH 576

Query: 558 TFFRNSINIWFQFLPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHV------MIYMFLGP 617
            +FR ++NI  QF+P+MIF+  LFGYL  +II KWC        H        I MFL  
Sbjct: 577 IYFRRTLNIILQFIPEMIFILCLFGYLVFMIIFKWCCFDVHVSQHAPSILIHFINMFLFN 636

Query: 618 TEDLAENQLFPGQKNVQIVLLLLALIAVPWMLLPKPFLLKKQHE--------------QR 677
             D +   L+  Q+ VQ   +++ALI+VPWMLL KPF+L+  H               + 
Sbjct: 637 YSDSSNAPLYKHQQEVQSFFVVMALISVPWMLLIKPFILRASHRKSQLQASRIQEDATEN 696

Query: 678 FQGQSYAPLPSADDSLELDSHH--DSHGHEEFEFSEVFVHQLIHTIEFVLGAVSNTASYL 737
            +G S +P   +      D+H   D HG EEF F +VFVHQ IHTIE+ LG +SNTASYL
Sbjct: 697 IEGDSSSPSSRSGQRTSADTHGALDDHG-EEFNFGDVFVHQAIHTIEYCLGCISNTASYL 756

Query: 738 RLWALSLAHSELSSVFYDKVL---VLSAGFNNVIILIVGIIVFIFATVGVLLLMETLSAF 782
           RLWALSLAH++LS V +  V+   + + G+  ++ + +   VF   TV +LL+ME LSAF
Sbjct: 757 RLWALSLAHAQLSEVLWTMVMNSGLQTRGWGGIVGVFIIFAVFAVLTVAILLIMEGLSAF 816

BLAST of Carg24675 vs. ExPASy TrEMBL
Match: A0A6J1F0E4 (V-type proton ATPase subunit a OS=Cucurbita moschata OX=3662 GN=LOC111438334 PE=3 SV=1)

HSP 1 Score: 1502.6 bits (3889), Expect = 0.0e+00
Identity = 770/778 (98.97%), Postives = 772/778 (99.23%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 71
           D  +  F  LNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI
Sbjct: 41  DLGLLQFKDLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 100

Query: 72  DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 131
           DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE
Sbjct: 101 DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 160

Query: 132 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 191
           SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ
Sbjct: 161 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 220

Query: 192 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 251
           AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA
Sbjct: 221 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 280

Query: 252 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 311
           EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL
Sbjct: 281 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 340

Query: 312 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 371
           VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD
Sbjct: 341 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 400

Query: 372 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 431
           AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT
Sbjct: 401 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 460

Query: 432 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 491
           EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS
Sbjct: 461 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 520

Query: 492 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 551
           TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF
Sbjct: 521 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 580

Query: 552 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 611
           LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV
Sbjct: 581 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 640

Query: 612 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 671
           LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLE+DSHHDSHGHEEFEFS
Sbjct: 641 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLEVDSHHDSHGHEEFEFS 700

Query: 672 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 731
           EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV
Sbjct: 701 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 760

Query: 732 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE
Sbjct: 761 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 818

BLAST of Carg24675 vs. ExPASy TrEMBL
Match: A0A6J1I378 (V-type proton ATPase subunit a OS=Cucurbita maxima OX=3661 GN=LOC111469500 PE=3 SV=1)

HSP 1 Score: 1494.2 bits (3867), Expect = 0.0e+00
Identity = 765/778 (98.33%), Postives = 771/778 (99.10%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 71
           D  +  F  LNVEKSPFQRTYAAQIKRCGEMARKL+FFKEQMLKAGLSSKSSVSQVDISI
Sbjct: 41  DLGLLQFKDLNVEKSPFQRTYAAQIKRCGEMARKLNFFKEQMLKAGLSSKSSVSQVDISI 100

Query: 72  DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 131
           DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVL KAGEFFS+AQSSAVEQQREFE
Sbjct: 101 DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLHKAGEFFSAAQSSAVEQQREFE 160

Query: 132 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 191
           SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ
Sbjct: 161 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 220

Query: 192 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 251
           AAV+DPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMI+
Sbjct: 221 AAVEDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIS 280

Query: 252 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 311
           EVSGKLAELKTTID+GLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL
Sbjct: 281 EVSGKLAELKTTIDLGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 340

Query: 312 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 371
           VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD
Sbjct: 341 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 400

Query: 372 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 431
           AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT
Sbjct: 401 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 460

Query: 432 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 491
           EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS
Sbjct: 461 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 520

Query: 492 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 551
           TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF
Sbjct: 521 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 580

Query: 552 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 611
           LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV
Sbjct: 581 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 640

Query: 612 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 671
           LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS
Sbjct: 641 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 700

Query: 672 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 731
           EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV
Sbjct: 701 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 760

Query: 732 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE
Sbjct: 761 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 818

BLAST of Carg24675 vs. ExPASy TrEMBL
Match: A0A0A0K0E0 (V-type proton ATPase subunit a OS=Cucumis sativus OX=3659 GN=Csa_7G004130 PE=3 SV=1)

HSP 1 Score: 1450.3 bits (3753), Expect = 0.0e+00
Identity = 733/778 (94.22%), Postives = 760/778 (97.69%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 71
           D  +  F  LN +KSPFQRTYAAQIKRCGEMARKL+FFKEQ+L+AGLSSKSSVSQVDI+I
Sbjct: 41  DLGLLQFKDLNADKSPFQRTYAAQIKRCGEMARKLNFFKEQILRAGLSSKSSVSQVDINI 100

Query: 72  DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 131
           DDLEVKLGELEAELVEINANSEKLQRSYNEL+EYKLVLQKAGEFF +AQSSAVEQQREFE
Sbjct: 101 DDLEVKLGELEAELVEINANSEKLQRSYNELLEYKLVLQKAGEFFIAAQSSAVEQQREFE 160

Query: 132 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 191
           SR+ GGDSIEVPLLLEQES  DQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ
Sbjct: 161 SRQTGGDSIEVPLLLEQESLVDQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 220

Query: 192 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 251
            AV+DPV DPISGEKVEKNVF+VFYSGERAKNKILKICEAFGANRYPFTED+GKQAQMIA
Sbjct: 221 TAVEDPVADPISGEKVEKNVFIVFYSGERAKNKILKICEAFGANRYPFTEDVGKQAQMIA 280

Query: 252 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 311
           EVSGKL+ELKTTID+GLLHRGNLL+ IGEHFENWN++ARKEKSIYHILNMLSLDVTKKCL
Sbjct: 281 EVSGKLSELKTTIDIGLLHRGNLLQTIGEHFENWNLLARKEKSIYHILNMLSLDVTKKCL 340

Query: 312 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 371
           VAEGWGPVFATKQIQDALQRAA+DSNSQVGPIFQVLLTTEAPPTYFRTNKFS+AFQEIVD
Sbjct: 341 VAEGWGPVFATKQIQDALQRAASDSNSQVGPIFQVLLTTEAPPTYFRTNKFSSAFQEIVD 400

Query: 372 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 431
           AYGVA+YQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT
Sbjct: 401 AYGVARYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 460

Query: 432 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 491
           EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAY CRSPDCSDSTTVGL+K GS
Sbjct: 461 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYACRSPDCSDSTTVGLLKVGS 520

Query: 492 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 551
           TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF
Sbjct: 521 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 580

Query: 552 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 611
           LPQMIFLNSLFGYLSLLIIIKWCTGS ADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV
Sbjct: 581 LPQMIFLNSLFGYLSLLIIIKWCTGSNADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 640

Query: 612 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 671
           LLLLAL+AVPWMLLPKPFLLK+QHEQRFQGQSYAPLPS DDSLELDSHHDSHGHEEFEFS
Sbjct: 641 LLLLALVAVPWMLLPKPFLLKRQHEQRFQGQSYAPLPSGDDSLELDSHHDSHGHEEFEFS 700

Query: 672 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 731
           E+FVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNN+IILIV
Sbjct: 701 EIFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNIIILIV 760

Query: 732 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKF+PFSFALLDEDD+
Sbjct: 761 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFHPFSFALLDEDDD 818

BLAST of Carg24675 vs. ExPASy TrEMBL
Match: A0A6J1HW75 (V-type proton ATPase subunit a OS=Cucurbita maxima OX=3661 GN=LOC111468405 PE=3 SV=1)

HSP 1 Score: 1449.5 bits (3751), Expect = 0.0e+00
Identity = 737/778 (94.73%), Postives = 757/778 (97.30%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 71
           D  +  F  LN EKSPFQRTYAAQIKRCGEMARKL+FFKEQMLKAGL SKSS+SQVDI+I
Sbjct: 41  DLGLLQFKDLNAEKSPFQRTYAAQIKRCGEMARKLNFFKEQMLKAGLPSKSSISQVDINI 100

Query: 72  DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 131
           DDLEVKLGELEAEL+EINANSEKLQRSYNELVEYKLVLQKAG+FF +AQSSA+EQQREFE
Sbjct: 101 DDLEVKLGELEAELIEINANSEKLQRSYNELVEYKLVLQKAGDFFGAAQSSAIEQQREFE 160

Query: 132 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 191
           SRRVGGDSIEVPLLLEQESS DQSKPVNLGFLSGLVPR KSMAFERILFRATRGNVFLKQ
Sbjct: 161 SRRVGGDSIEVPLLLEQESSVDQSKPVNLGFLSGLVPRGKSMAFERILFRATRGNVFLKQ 220

Query: 192 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 251
           A V+DPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQ+QMIA
Sbjct: 221 AVVEDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQSQMIA 280

Query: 252 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 311
           EVSGKLAELKTTIDVGLLHR NLL+ I EHFENWN++ARKEKSIYHILNMLSLDVTK+CL
Sbjct: 281 EVSGKLAELKTTIDVGLLHRANLLQTIREHFENWNLLARKEKSIYHILNMLSLDVTKQCL 340

Query: 312 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 371
           VAEGWGPVFATKQIQ+ALQRAA+DSNSQVG IFQVLLTTEAPPTYFRTNKFSTAFQEIVD
Sbjct: 341 VAEGWGPVFATKQIQEALQRAASDSNSQVGAIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 400

Query: 372 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 431
           AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT
Sbjct: 401 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 460

Query: 432 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 491
           EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPF LFGRSAY CRSPDCSDSTTVGLIKAGS
Sbjct: 461 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFELFGRSAYACRSPDCSDSTTVGLIKAGS 520

Query: 492 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 551
           TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFF+NSINIWFQF
Sbjct: 521 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFKNSINIWFQF 580

Query: 552 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 611
           LPQMIFLNSLFGYLSLLIIIKWCTGS ADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV
Sbjct: 581 LPQMIFLNSLFGYLSLLIIIKWCTGSNADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 640

Query: 612 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 671
           LLLLAL+AVPWMLLPKPFLLKKQHEQRFQGQSYAPLPS DDSLELDSHHDSHGHEEFEFS
Sbjct: 641 LLLLALVAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSGDDSLELDSHHDSHGHEEFEFS 700

Query: 672 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 731
           EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNN++ILIV
Sbjct: 701 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNIVILIV 760

Query: 732 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKF PFSFALLDEDDE
Sbjct: 761 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFLPFSFALLDEDDE 818

BLAST of Carg24675 vs. ExPASy TrEMBL
Match: A0A1S3BIV8 (V-type proton ATPase subunit a OS=Cucumis melo OX=3656 GN=LOC103490358 PE=3 SV=1)

HSP 1 Score: 1449.5 bits (3751), Expect = 0.0e+00
Identity = 732/778 (94.09%), Postives = 760/778 (97.69%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVSQVDISI 71
           D  +  F  LN +KSPFQRTYAAQIKRCGEMARKL+FFKEQ+LKAGLSSKSSVSQVDI+I
Sbjct: 41  DLGLLQFKDLNADKSPFQRTYAAQIKRCGEMARKLNFFKEQILKAGLSSKSSVSQVDINI 100

Query: 72  DDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREFE 131
           DDLEVKLGELEAELVEINANSEKLQRSYNEL+EYKLVLQKAGEFF++AQSSAVEQQREFE
Sbjct: 101 DDLEVKLGELEAELVEINANSEKLQRSYNELLEYKLVLQKAGEFFTAAQSSAVEQQREFE 160

Query: 132 SRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 191
           SRR GGDSIEVPLLLEQES  DQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ
Sbjct: 161 SRRTGGDSIEVPLLLEQESLVDQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQ 220

Query: 192 AAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIA 251
             V+DPV DP+SGEKVEKNVF+VFYSGERAK+KILKICEAFGANRYPFTED+GKQAQMIA
Sbjct: 221 TEVEDPVADPVSGEKVEKNVFIVFYSGERAKSKILKICEAFGANRYPFTEDVGKQAQMIA 280

Query: 252 EVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCL 311
           EVSGKL+ELKTTID+GLLHRGNLL+ IGEHFENWN++ARKEKSIYHILNMLSLDVTKKCL
Sbjct: 281 EVSGKLSELKTTIDIGLLHRGNLLQTIGEHFENWNLLARKEKSIYHILNMLSLDVTKKCL 340

Query: 312 VAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVD 371
           VAEGWGPVFATKQIQDALQRAA+DSNSQVGPIFQVLLTTEAPPTYFRTNKFS+AFQEIVD
Sbjct: 341 VAEGWGPVFATKQIQDALQRAASDSNSQVGPIFQVLLTTEAPPTYFRTNKFSSAFQEIVD 400

Query: 372 AYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDIT 431
           AYGVA+YQEANPGVYTIVTFPFLFAVMFGDWGHGICL LATLYFILREKKLSSQKLGDIT
Sbjct: 401 AYGVARYQEANPGVYTIVTFPFLFAVMFGDWGHGICLFLATLYFILREKKLSSQKLGDIT 460

Query: 432 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGS 491
           EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTT+GLIK GS
Sbjct: 461 EMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTMGLIKVGS 520

Query: 492 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 551
           TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF
Sbjct: 521 TYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQF 580

Query: 552 LPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIV 611
           LPQMIFLNSLFGYLSLLIIIKWCTGS ADLYHVMIYMFLGPT+DLAENQLFPGQKNVQIV
Sbjct: 581 LPQMIFLNSLFGYLSLLIIIKWCTGSNADLYHVMIYMFLGPTDDLAENQLFPGQKNVQIV 640

Query: 612 LLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEFS 671
           LLLLAL+AVPWMLLPKPFLLK+QHEQRFQGQSYAPLPS DDSLELDSHHDSHGHEEFEFS
Sbjct: 641 LLLLALVAVPWMLLPKPFLLKRQHEQRFQGQSYAPLPSGDDSLELDSHHDSHGHEEFEFS 700

Query: 672 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIV 731
           EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNN+IILIV
Sbjct: 701 EVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNIIILIV 760

Query: 732 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKF+PFSFALLDEDD+
Sbjct: 761 GIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFHPFSFALLDEDDD 818

BLAST of Carg24675 vs. TAIR 10
Match: AT4G39080.1 (vacuolar proton ATPase A3 )

HSP 1 Score: 1202.2 bits (3109), Expect = 0.0e+00
Identity = 605/780 (77.56%), Postives = 689/780 (88.33%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKS-SVSQVDIS 71
           D  +  F  LN EKSPFQRTYAAQIKRCGEMARK+ FF++QM KAG+ +K     + DI 
Sbjct: 43  DLGLVQFKDLNSEKSPFQRTYAAQIKRCGEMARKIRFFRDQMSKAGVPAKEMQGKENDID 102

Query: 72  IDDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREF 131
           +DD+EVKLGELEAELVEINAN++KLQRSYNEL+EYKLVLQKAGEFFSSA  SA +QQRE 
Sbjct: 103 LDDVEVKLGELEAELVEINANNDKLQRSYNELMEYKLVLQKAGEFFSSAHRSAADQQRET 162

Query: 132 ESRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLK 191
           ES++ G D +E P LL++E S D +K V LGFL+GLVPREKSM FERILFRATRGN+F++
Sbjct: 163 ESQQAGEDLLESP-LLQEEKSIDSTKQVKLGFLTGLVPREKSMVFERILFRATRGNIFIR 222

Query: 192 QAAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMI 251
           Q  +++PV DP SGEK EKNVFVVFYSGERAK+KILKICEAFGANRYPF+EDLG+QAQMI
Sbjct: 223 QTVIEEPVIDPNSGEKAEKNVFVVFYSGERAKSKILKICEAFGANRYPFSEDLGRQAQMI 282

Query: 252 AEVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKC 311
            EVSG+L+ELKTTID GL  R  LL+ IG+ FE WN+  RKEK+IYH LNMLSLDVTKKC
Sbjct: 283 TEVSGRLSELKTTIDAGLGQRNILLQTIGDKFELWNLKVRKEKAIYHTLNMLSLDVTKKC 342

Query: 312 LVAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIV 371
           LVAEGW PVFA+++IQDALQRAA DSNSQVG IFQVL T E+PPTYFRTNKF++A QEIV
Sbjct: 343 LVAEGWSPVFASREIQDALQRAAVDSNSQVGSIFQVLRTKESPPTYFRTNKFTSAIQEIV 402

Query: 372 DAYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDI 431
           DAYGVAKYQEANPGV+TIVTFPFLFAVMFGDWGHGIC+LLAT+Y IL+EKKL+SQKLGDI
Sbjct: 403 DAYGVAKYQEANPGVFTIVTFPFLFAVMFGDWGHGICILLATMYLILKEKKLASQKLGDI 462

Query: 432 TEMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAG 491
            EMAFGGRYVILMMSLFSIYTGLIYNEFFS+PF LF  SAY+CR   CS++TT+GLIK  
Sbjct: 463 MEMAFGGRYVILMMSLFSIYTGLIYNEFFSIPFPLFAPSAYDCRDVSCSEATTIGLIKVR 522

Query: 492 STYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQ 551
            TYPFGLDPVWHG+RSELPFLNSLKMKMSILLGV+QMNLGII+SYFNA FF++S+NIWFQ
Sbjct: 523 DTYPFGLDPVWHGSRSELPFLNSLKMKMSILLGVSQMNLGIIMSYFNARFFKSSVNIWFQ 582

Query: 552 FLPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQI 611
           F+PQMIFLNSLFGYLS+LIIIKWCTGS+ADLYHVMIYMFL P ++L ENQLFP QK +Q+
Sbjct: 583 FIPQMIFLNSLFGYLSVLIIIKWCTGSQADLYHVMIYMFLSPMDELGENQLFPHQKTLQL 642

Query: 612 VLLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSH-HDSHGHEEFE 671
           VLL LAL++VP MLLPKPF+LKKQHE R QGQ+YAPL   D+SL ++++   SHGHEEFE
Sbjct: 643 VLLFLALVSVPCMLLPKPFILKKQHEARHQGQAYAPLDETDESLHVETNGGGSHGHEEFE 702

Query: 672 FSEVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIIL 731
           FSE+FVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFY+KVL+L+ G+NN +IL
Sbjct: 703 FSEIFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYEKVLLLAWGYNNPLIL 762

Query: 732 IVGIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           IVG++VFIFATVGVLL+METLSAFLHALRLHWVEFQNKFYEGDGYKF PF+F     +DE
Sbjct: 763 IVGVLVFIFATVGVLLVMETLSAFLHALRLHWVEFQNKFYEGDGYKFAPFTFIFTANEDE 821

BLAST of Carg24675 vs. TAIR 10
Match: AT2G21410.1 (vacuolar proton ATPase A2 )

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 598/779 (76.77%), Postives = 678/779 (87.03%), Query Frame = 0

Query: 12  DHPMYYFFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVS-QVDIS 71
           D  +  F  LN EKSPFQRTYAAQIKRCGEMARK+ FFKEQM KAG++ K ++  + DI 
Sbjct: 44  DLGLVQFKDLNSEKSPFQRTYAAQIKRCGEMARKIRFFKEQMSKAGVTPKETLDRENDID 103

Query: 72  IDDLEVKLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREF 131
           +DD+EVKL ELEAELVEINAN++KLQRSYNELVEYKLVL+KAGEFF+SA  SA  QQ E 
Sbjct: 104 LDDVEVKLEELEAELVEINANNDKLQRSYNELVEYKLVLEKAGEFFASAHRSATAQQSEI 163

Query: 132 ESRRVGGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLK 191
           E+ +VG D +E P LL++E S D +K V LGFL+GLVPREKSM FERILFRATRGN+F++
Sbjct: 164 ETEQVGEDLLEAP-LLQEEKSVDPTKQVKLGFLTGLVPREKSMVFERILFRATRGNIFIR 223

Query: 192 QAAVDDPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMI 251
           Q+ +++ V DP SGEK EKNVFVVFYSGERAK+KILKICEAFGANRYPF+EDLGKQAQM+
Sbjct: 224 QSVIEESVVDPNSGEKAEKNVFVVFYSGERAKSKILKICEAFGANRYPFSEDLGKQAQMM 283

Query: 252 AEVSGKLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKC 311
            EVSG+L+ELKTTI  GL  R  LL+ IG+ FE WN+  RKEK+IYH LNMLSLDVTKKC
Sbjct: 284 TEVSGRLSELKTTIGAGLDQRNILLETIGDKFEQWNLKIRKEKAIYHTLNMLSLDVTKKC 343

Query: 312 LVAEGWGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIV 371
           LV EGW PVFA  +IQDAL RAA DSNSQVG IFQVL T E PPT+FRTNKF+TAFQEIV
Sbjct: 344 LVGEGWSPVFAATEIQDALHRAAVDSNSQVGSIFQVLRTKEMPPTFFRTNKFTTAFQEIV 403

Query: 372 DAYGVAKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDI 431
           DAYGVAKYQEANP V+TIVTFPFLFAVMFGDWGHGICLLLAT+Y ILREKKLSSQKLGDI
Sbjct: 404 DAYGVAKYQEANPSVFTIVTFPFLFAVMFGDWGHGICLLLATMYLILREKKLSSQKLGDI 463

Query: 432 TEMAFGGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAG 491
            EMAFGGRYVI MMSLFSIYTGLIYNEFFS+P+ LF  SAY+CR   CS++TT+GLIK  
Sbjct: 464 MEMAFGGRYVIFMMSLFSIYTGLIYNEFFSIPYPLFASSAYDCRDVSCSEATTIGLIKTR 523

Query: 492 STYPFGLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQ 551
            TYPFG+DPVWHGTRSELPFLNSLKMKMSIL+GVAQMNLGII+S+FNA FF++++NIWFQ
Sbjct: 524 DTYPFGVDPVWHGTRSELPFLNSLKMKMSILIGVAQMNLGIIMSFFNAKFFKSAVNIWFQ 583

Query: 552 FLPQMIFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQI 611
           F+PQMIFLN LFGYLS+LIIIKWCTGS+ADLYHVMIYMFL P +DL ENQLFP QK VQ+
Sbjct: 584 FVPQMIFLNCLFGYLSVLIIIKWCTGSQADLYHVMIYMFLSPMDDLGENQLFPNQKIVQL 643

Query: 612 VLLLLALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELDSHHDSHGHEEFEF 671
             L LAL++VPWMLLPKPF+LKKQHE R QG SYA L   D+SL+++++   HGHEEFEF
Sbjct: 644 TFLFLALVSVPWMLLPKPFILKKQHEARHQGLSYAQLDETDESLQVETNGGGHGHEEFEF 703

Query: 672 SEVFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILI 731
           SE+FVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFY+KVL+++ GFNNV I I
Sbjct: 704 SEIFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYEKVLLMAWGFNNVFIWI 763

Query: 732 VGIIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALLDEDDE 790
           VGI+VFIFATVGVLL+METLSAFLHALRLHWVE+QNKFYEGDGYKF PF+F L+  +DE
Sbjct: 764 VGILVFIFATVGVLLVMETLSAFLHALRLHWVEYQNKFYEGDGYKFAPFTFTLVGNEDE 821

BLAST of Carg24675 vs. TAIR 10
Match: AT2G28520.1 (vacuolar proton ATPase A1 )

HSP 1 Score: 966.8 bits (2498), Expect = 1.0e-281
Identity = 485/772 (62.82%), Postives = 604/772 (78.24%), Query Frame = 0

Query: 18  FFQLNVEKSPFQRTYAAQIKRCGEMARKLHFFKEQMLKAGLSSKSSVS-QVDISIDDLEV 77
           F  LN +KSPFQRT+A Q+KRCGEM+RKL FFK+Q+ KAGL     +  + DI++ DLE 
Sbjct: 46  FRDLNADKSPFQRTFANQVKRCGEMSRKLRFFKDQIDKAGLRCSPRLEIEPDIALGDLER 105

Query: 78  KLGELEAELVEINANSEKLQRSYNELVEYKLVLQKAGEFFSSAQSSAVEQQREF-ESRRV 137
           +L + E E++E+N+NSEKL+++YNEL+E+K+VL+KA  F  S+ + A+ ++ E  ES   
Sbjct: 106 QLADHEHEVLEMNSNSEKLRQTYNELLEFKIVLEKASGFLVSSNTHAIGEEIELHESTYS 165

Query: 138 GGDSIEVPLLLEQESSADQSKPVNLGFLSGLVPREKSMAFERILFRATRGNVFLKQAAVD 197
               IE   LLEQE +   S    L F+SG++ ++K + FER+LFRATRGN+   Q   D
Sbjct: 166 NNGFIETASLLEQEMNPGHSNQSGLRFISGIINKDKLLKFERMLFRATRGNMLFNQTTSD 225

Query: 198 DPVPDPISGEKVEKNVFVVFYSGERAKNKILKICEAFGANRYPFTEDLGKQAQMIAEVSG 257
           + + DP + E VEK VFVVF+SGE+A+ KILKICEAFGAN YP  ED  KQ Q+  EV  
Sbjct: 226 EEIMDPSTSEMVEKVVFVVFFSGEQARTKILKICEAFGANCYPVPEDTTKQRQLTREVLS 285

Query: 258 KLAELKTTIDVGLLHRGNLLKNIGEHFENWNIMARKEKSIYHILNMLSLDVTKKCLVAEG 317
           +L++L+ T+D G  HR N L ++G    NW    R+EK++Y  LNML+ DVTKKCLV EG
Sbjct: 286 RLSDLEATLDAGTRHRNNALNSVGYSLTNWITTVRREKAVYDTLNMLNFDVTKKCLVGEG 345

Query: 318 WGPVFATKQIQDALQRAATDSNSQVGPIFQVLLTTEAPPTYFRTNKFSTAFQEIVDAYGV 377
           W P FA  QI + LQRA  DS+SQVG IF V+   E+PPTYFRTNK + AFQEI+DAYGV
Sbjct: 346 WCPTFAKTQIHEVLQRATFDSSSQVGVIFHVMQAVESPPTYFRTNKLTNAFQEIIDAYGV 405

Query: 378 AKYQEANPGVYTIVTFPFLFAVMFGDWGHGICLLLATLYFILREKKLSSQKLGDITEMAF 437
           A+YQEANP VY++VT+PFLFAVMFGDWGHG+CLLL  LY + RE+KLS+QKLG   EM F
Sbjct: 406 ARYQEANPAVYSVVTYPFLFAVMFGDWGHGLCLLLGALYLLARERKLSTQKLGSFMEMLF 465

Query: 438 GGRYVILMMSLFSIYTGLIYNEFFSVPFGLFGRSAYECRSPDCSDSTTVGLIKAGSTYPF 497
           GGRYVIL+M+LFSIY GLIYNEFFSVPF +FG SAY+CR   CSD+ TVGLIK    YPF
Sbjct: 466 GGRYVILLMALFSIYCGLIYNEFFSVPFHIFGGSAYKCRDTTCSDAYTVGLIKYRDPYPF 525

Query: 498 GLDPVWHGTRSELPFLNSLKMKMSILLGVAQMNLGIIISYFNATFFRNSINIWFQFLPQM 557
           G+DP W G+R+ELP+LNSLKMKMSILLG+AQMNLG+I+S+FNA FF +S++I +QF+PQM
Sbjct: 526 GVDPSWRGSRTELPYLNSLKMKMSILLGIAQMNLGLILSFFNARFFGSSLDIRYQFIPQM 585

Query: 558 IFLNSLFGYLSLLIIIKWCTGSKADLYHVMIYMFLGPTEDLAENQLFPGQKNVQIVLLLL 617
           IFLNSLFGYLSLLIIIKWCTGS+ADLYHVMIYMFL PTE+L EN+LF GQ+ +QIVLLLL
Sbjct: 586 IFLNSLFGYLSLLIIIKWCTGSQADLYHVMIYMFLSPTEELGENELFWGQRPLQIVLLLL 645

Query: 618 ALIAVPWMLLPKPFLLKKQHEQRFQGQSYAPLPSADDSLELD---SHHDSHGHEEFEFSE 677
           A IAVPWML PKPF L+K H +RFQG++Y  L S++  L+++   +    H  EEF FSE
Sbjct: 646 AFIAVPWMLFPKPFALRKIHMERFQGRTYGVLVSSEVDLDVEPDSARGGGHHEEEFNFSE 705

Query: 678 VFVHQLIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFYDKVLVLSAGFNNVIILIVG 737
           +FVHQLIH+IEFVLG+VSNTASYLRLWALSLAHSELS+VFY+KVL+L+ G+ N++I ++G
Sbjct: 706 IFVHQLIHSIEFVLGSVSNTASYLRLWALSLAHSELSTVFYEKVLLLAWGYENILIRLIG 765

Query: 738 IIVFIFATVGVLLLMETLSAFLHALRLHWVEFQNKFYEGDGYKFNPFSFALL 785
           + VF FAT  +LL+METLSAFLHALRLHWVEF  KF+ GDGYKF PFSFAL+
Sbjct: 766 VAVFAFATAFILLMMETLSAFLHALRLHWVEFMGKFFNGDGYKFKPFSFALI 817