Carg24551 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg24551
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Membrane-anchored ubiquitin-fold protein
Location	Carg_Chr11: 72865 .. 76407 (+)
RNA-Seq Expression	Carg24551
Synteny	Carg24551

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATATATATCGAATTTCTTATTGAATAATATTCTTCTTTCTGAAAAGCCTGCAGCTGAGAGGAAACTCTGCCTTGGCCTTGCCTGATCGACGGCTTCACAAAGGTCTCTTCTCTCTTCTCTTGATTGTCGTTTTCTTTTCACTTTATTCAAAATTACTTCCCTTTTCGGATCTTTCATGGTGTTCACTCCTGCAATTGCTTCAGCTTCGTTGCTGTTGGAATTCCTGTGAATTTGTTCTAGGGCACCGGATATTGGGCCGCGTCTCTGATCAATTCAAGGTCGGGATCCAACACTTATACCTGATTGTGAAATACTCAACTCAGATGCCGGAGGACGATTTGATTGACATTAAGTTCAGGCTCTACGATGGTTCTGATATTGGACCATTTCGATACTCGTCCGCATCGACAATAGATGTGCTTAAGCAGAGAATAGTGTCCGATTGGCCCAAAGGTTCCAGCTTAGCTCTTCATCTTCTCCTTATTCTTTTCAATTATAAGTACTCAATTTGTAATCATTTCTCACATTTAGGTTTCTGTATGTCCTTTGAATGCAAACGCTAAGTAGACTTCCGGCTTTCCTAATTGCATGTCTAGAGTCTGTTGATCCTCCTTTTTTTAGTACTATAGGCTGCTAGCTTAAGAAATCTATTTTTAGGACTAATATTTTATTCTTGGCAGACAGCTCTGCGTAGATATTTTATTCTTGATTTACGCGCTCGTTCAGAGGACGCATGTTCAATTCTTTGATGTGTGTCGTTTACCATTAGGAACTCATGCCTGTGTTTGAGGTTTCTCTGAAGAAGTGTCTTTCTTTCACTGGGCTTCTGATCTTCTTCTTTTTTCCTCTGCATTTGTTTTCATATTGATTCTCCTTTTCCCTTTTGGGACGATTTTTCTTTACCTTTTAGGCAAAACAATCACTCCAAAGGCAGCAAGTGAAGTAAAACTGATAAGTTCTGGAAAAATTTTGGAAAATAACAAGACTGTTGGTCAGTGTAAATTACCTTTTGGTGAGTTCACTGGAGGAGTTACTATAATGCACGTTGTTGTACAGCCATCCCTAGCAAAAGCTAAAACAGGTGATTACATTTGAGATTTTAGCTATAAATATAAATTTCTTGTTCATAGATTGTTCCTTTTAAAATTTTACAGTAATACGGTATATTTTCCATTTTGTCCTATACCCGATGGTTTTTCAAATACTCTGGATGTAGTTCGACAAGTCTATATAAATGAGGTCTTAGGAGTTGGAACCGTATTCTGAACTGATACAGTTATCCATTTGTTGGTCTTTTTTGGCTTGAACTTGGGTCAATACTCTTCTGATGCTAATCACTAACAACTGTTTTTGTTGCTCTTTCTGAAGTTAATGAGATATAACAATTTCAAAATCATTCTTAGAGCATTCAATCAGCTTTGACTAGTGCTTTTGAGTTAGAAGTTTGACTTTGCTAACTAGAATACCGAATCTTAGCAAGTGGTCCCATGAGTTAGCTATCATGAGCCTTTCAGAGAACCTTCTTTCTTCATTTATTCTGCCGCAGATGTTATCCCTTTTTTCTAAGCTTGTCCTGAACTACTGCCTGTGGCTTTTAAATGAAGAAGTGTTTTTGAGTGCTTTCTTTGAATGGTTAAGACAATAACTGTTTCAGGTTGTAGCCAAACCATTTCATTTAACCCAAAGTCATAGGATCATCTCATTGACTATTACTCCTCTACAAAGTATGCAGCGAAAAGTGAAAGTAGTCCATGGGTTGACCTAGTGGTTAAGAAGGCCAAATGAGAAGAGTTGAAGTGTGTGTATGGTCCTCAAATTTTGTGTCCATTTGGTTCATAAACTTTTAAAATTACAAATTTTAATTTATACCTATTAGATCAATTAGTTTTAAAAATTTTCAAATTTTTTGGTGACCTAAACACAAAGTTGAAAATTTCAAGACTTATTAGATATTTTTGAAAGCTGAAGGACCAAATAAACACAAGTTGAGAGACTAAACTTGTTATTTAAGATAGATATTTTCTAAGGGCTTAGGCTGGTTTATTATTCGGATCTACTAGTAATGATAAAAATTCTTGTCAGCTTTGCAGATTTAACTAAAGATTCAAAATATCCACTCCTACCCATTCTTATTTTTTTCCTGATTTGGCCCTTTTCTCTACTCTTGCTTCTTCTCTCTTCAATATTTTTTCCTTTTTATCTTTTTTCTTCTTTTCATATTCTTTTATCATTGGAAAAACTAGAATAAACTAGAGAAGAAAGAACTTGGATTGTTGTGAGGGCCGAGGAAAATCAAAAAAATCTTCCAGATTTATTTATTTTATTTTGTTAAGTTTACAGTTAATTAACTGTTGATTACTATTAAAATCTGTTATATTATTTTTATTATACAATATAGAAATTATGTATTAACTAGCCTTATGTAATTGACTTGCTCTAATTTCCATATTGCAAAGATTTAATTTTGATTAATACATTAACTAATATCTCACGATTACATATGCTTACTTCTGATTCTATAAGAACCTCTCTTATTTCCATGAAACATGACTATAAGTTTCTATAATTGCTTAGAATTTCTGTATGTTTCATCTTCACATATGATTACTTGGGATTCTATGAAAACATCGTTTATTTCCATGAAGGTTGACAAAGTTGTTATGAAAGCTAAAACATACTTCTGATTCCAAAATAAAGAGGGTATAGTCAATCAAATTTAAACTAAGTTTGCCAATGTGGCAGTCTAGGATCTCCTTTGTGGATTTGTTTTCTTCTGTTTGATTATCAATTGTTCTTTCTTGATTCCATTGAATGCATATCCAAACAATATCCACAGCTACGTATGTACCCCTTGACGAGCAAGAAGAATGAAAAATAGAAAGTTACCTGTATAAATAAGTTTATGTGACTGCTATGGGTTTCTAGCCAATTTTTGAAAATGATCATTGTAAAACTAGATTAAATCATGTAATCACTATCCAAATTTGTTTTTCCGATGAGTGTGGTAATTCTTTGCTGTGATATCAAATATTTACTGAGATATGTTGATGATACAGAGAAAAAGACTGACAATTCGCAACAGAAGATCGTGTGCTCCTGTTCCATACTTTGAAACACAACACGAATGATATGATTCGTTTGGTAAATCATCTTTCAAAACGTGGTTGCTATCGGCCATATTCTTGTGTATAAATGGTTTCTGGTAAATTGGTAATGATGTGTTTAGGAACATTGACATGAAGGACATGTTTCTCTTTTATGTGATTGTGAAGTTTTCATATGATATTTCAAATTCTTTGGTTACTAAGGTCATGCTGTATTCTGTTTTTCCCATGTGCGTTTCTCTTTTGGTACAGGAGAAGCTAGGTCATCTCTCTTAGAGGGTTTGCAGATAGAAGAAGAGGGCTATTTATAGATTTTGAGATGAGTTCAAAGGTCTAGAATACTCCTTAGGGATACTGCTCGAGTACTAAGGCAATTAGGGTAATTTTGGAAAGTTGCAAGAGTATTTCTATCCCTATACTTTTTTCCCCATCAAAAGAA

mRNA sequence

ATATATATCGAATTTCTTATTGAATAATATTCTTCTTTCTGAAAAGCCTGCAGCTGAGAGGAAACTCTGCCTTGGCCTTGCCTGATCGACGGCTTCACAAAGGGCACCGGATATTGGGCCGCGTCTCTGATCAATTCAAGGTCGGGATCCAACACTTATACCTGATTGTGAAATACTCAACTCAGATGCCGGAGGACGATTTGATTGACATTAAGTTCAGGCTCTACGATGGTTCTGATATTGGACCATTTCGATACTCGTCCGCATCGACAATAGATGTGCTTAAGCAGAGAATAGTGTCCGATTGGCCCAAAGAAGTGTCTTTCTTTCACTGGGCTTCTGATCTTCTTCTTTTTTCCTCTGCATTTGTTTTCATATTGATTCTCCTTTTCCCTTTTGGGACGATTTTTCTTTACCTTTTAGGCAAAACAATCACTCCAAAGGCAGCAAGTGAAGTAAAACTGATAAGTTCTGGAAAAATTTTGGAAAATAACAAGACTGTTGGTCAGTGTAAATTACCTTTTGGTGAGTTCACTGGAGGAGTTACTATAATGCACGTTGTTGTACAGCCATCCCTAGCAAAAGCTAAAACAGGAGAAGCTAGGTCATCTCTCTTAGAGGGTTTGCAGATAGAAGAAGAGGGCTATTTATAGATTTTGAGATGAGTTCAAAGGTCTAGAATACTCCTTAGGGATACTGCTCGAGTACTAAGGCAATTAGGGTAATTTTGGAAAGTTGCAAGAGTATTTCTATCCCTATACTTTTTTCCCCATCAAAAGAA

Coding sequence (CDS)

ATGCCGGAGGACGATTTGATTGACATTAAGTTCAGGCTCTACGATGGTTCTGATATTGGACCATTTCGATACTCGTCCGCATCGACAATAGATGTGCTTAAGCAGAGAATAGTGTCCGATTGGCCCAAAGAAGTGTCTTTCTTTCACTGGGCTTCTGATCTTCTTCTTTTTTCCTCTGCATTTGTTTTCATATTGATTCTCCTTTTCCCTTTTGGGACGATTTTTCTTTACCTTTTAGGCAAAACAATCACTCCAAAGGCAGCAAGTGAAGTAAAACTGATAAGTTCTGGAAAAATTTTGGAAAATAACAAGACTGTTGGTCAGTGTAAATTACCTTTTGGTGAGTTCACTGGAGGAGTTACTATAATGCACGTTGTTGTACAGCCATCCCTAGCAAAAGCTAAAACAGGAGAAGCTAGGTCATCTCTCTTAGAGGGTTTGCAGATAGAAGAAGAGGGCTATTTATAG

Protein sequence

MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSAFVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGVTIMHVVVQPSLAKAKTGEARSSLLEGLQIEEEGYL

Homology

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BLAST of Carg24551 vs. NCBI nr
Match: KAG7021381.1 (Membrane-anchored ubiquitin-fold protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 305.8 bits (782), Expect = 2.1e-79
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA
Sbjct: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
           FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV
Sbjct: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120

Query: 121 TIMHVVVQPSLAKAKTGEARSSLLEGLQIEEEGYL 156
           TIMHVVVQPSLAKAKTGEARSSLLEGLQIEEEGYL
Sbjct: 121 TIMHVVVQPSLAKAKTGEARSSLLEGLQIEEEGYL 155

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BLAST of Carg24551 vs. NCBI nr
Match: KAA0057753.1 (membrane-anchored ubiquitin-fold protein 3 [Cucumis melo var. makuwa] >TYJ98433.1 membrane-anchored ubiquitin-fold protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 191.0 bits (484), Expect = 7.4e-45
Identity = 101/138 (73.19%), Postives = 102/138 (73.91%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK                 
Sbjct: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK----------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                              GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV
Sbjct: 61  -------------------GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 102

Query: 121 TIMHVVVQPSLAKAKTGE 139
           TIMHVVVQPSLAKAKTG+
Sbjct: 121 TIMHVVVQPSLAKAKTGD 102

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BLAST of Carg24551 vs. NCBI nr
Match: XP_008464397.1 (PREDICTED: membrane-anchored ubiquitin-fold protein 3 [Cucumis melo] >XP_008464399.1 PREDICTED: membrane-anchored ubiquitin-fold protein 3 [Cucumis melo] >XP_008464400.1 PREDICTED: membrane-anchored ubiquitin-fold protein 3 [Cucumis melo] >XP_008464401.1 PREDICTED: membrane-anchored ubiquitin-fold protein 3 [Cucumis melo] >XP_016903199.1 PREDICTED: membrane-anchored ubiquitin-fold protein 3 [Cucumis melo] >XP_022932632.1 membrane-anchored ubiquitin-fold protein 3-like [Cucurbita moschata] >XP_022932642.1 membrane-anchored ubiquitin-fold protein 3-like [Cucurbita moschata] >XP_022974355.1 membrane-anchored ubiquitin-fold protein 3-like [Cucurbita maxima] >XP_022974363.1 membrane-anchored ubiquitin-fold protein 3-like [Cucurbita maxima] >XP_023531895.1 membrane-anchored ubiquitin-fold protein 3-like [Cucurbita pepo subsp. pepo] >XP_023531896.1 membrane-anchored ubiquitin-fold protein 3-like [Cucurbita pepo subsp. pepo] >XP_038879256.1 membrane-anchored ubiquitin-fold protein 3-like [Benincasa hispida] >KAG6587397.1 Membrane-anchored ubiquitin-fold protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 188.0 bits (476), Expect = 6.3e-44
Identity = 100/136 (73.53%), Postives = 100/136 (73.53%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK                 
Sbjct: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK----------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                              GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV
Sbjct: 61  -------------------GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 100

Query: 121 TIMHVVVQPSLAKAKT 137
           TIMHVVVQPSLAKAKT
Sbjct: 121 TIMHVVVQPSLAKAKT 100

[Close]

BLAST of Carg24551 vs. NCBI nr
Match: XP_031745197.1 (membrane-anchored ubiquitin-fold protein 3 [Cucumis sativus] >XP_031745199.1 membrane-anchored ubiquitin-fold protein 3 [Cucumis sativus] >XP_031745202.1 membrane-anchored ubiquitin-fold protein 3 [Cucumis sativus] >KGN63460.1 hypothetical protein Csa_013839 [Cucumis sativus])

HSP 1 Score: 187.6 bits (475), Expect = 8.2e-44
Identity = 99/136 (72.79%), Postives = 100/136 (73.53%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK                 
Sbjct: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK----------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                              GKTITPKAASE+KLISSGKILENNKTVGQCKLPFGEFTGGV
Sbjct: 61  -------------------GKTITPKAASEIKLISSGKILENNKTVGQCKLPFGEFTGGV 100

Query: 121 TIMHVVVQPSLAKAKT 137
           TIMHVVVQPSLAKAKT
Sbjct: 121 TIMHVVVQPSLAKAKT 100

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BLAST of Carg24551 vs. NCBI nr
Match: XP_022134657.1 (membrane-anchored ubiquitin-fold protein 3-like [Momordica charantia] >XP_022134659.1 membrane-anchored ubiquitin-fold protein 3-like [Momordica charantia] >XP_022134660.1 membrane-anchored ubiquitin-fold protein 3-like [Momordica charantia])

HSP 1 Score: 185.3 bits (469), Expect = 4.1e-43
Identity = 98/136 (72.06%), Postives = 99/136 (72.79%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIV DWPK                 
Sbjct: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVXDWPK----------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                              GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV
Sbjct: 61  -------------------GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 100

Query: 121 TIMHVVVQPSLAKAKT 137
           TIMHVVVQPS+AKAKT
Sbjct: 121 TIMHVVVQPSIAKAKT 100

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BLAST of Carg24551 vs. ExPASy Swiss-Prot
Match: Q9SW27 (Membrane-anchored ubiquitin-fold protein 3 OS=Arabidopsis thaliana OX=3702 GN=MUB3 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.4e-38
Identity = 81/136 (59.56%), Postives = 91/136 (66.91%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPE++ IDIKFRLYDGSDIGPFRYS+AST+D LKQR+VSDWPK                 
Sbjct: 1   MPEEESIDIKFRLYDGSDIGPFRYSAASTVDFLKQRVVSDWPK----------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                              GKT+ PK  +EVKLISSGKILENNKTVGQCK PFG+  GGV
Sbjct: 61  -------------------GKTVVPKGINEVKLISSGKILENNKTVGQCKTPFGDIAGGV 100

Query: 121 TIMHVVVQPSLAKAKT 137
            +MHVVVQPSLAK+KT
Sbjct: 121 IVMHVVVQPSLAKSKT 100

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BLAST of Carg24551 vs. ExPASy Swiss-Prot
Match: Q9LSD8 (Membrane-anchored ubiquitin-fold protein 4 OS=Arabidopsis thaliana OX=3702 GN=MUB4 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.5e-27
Identity = 62/135 (45.93%), Postives = 81/135 (60.00%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPE+DL+++KFRLYDGSD+GPF+YS  +T+ +LK+RIVS+WPK+                
Sbjct: 1   MPEEDLVELKFRLYDGSDVGPFQYSPTATVSMLKERIVSEWPKD---------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                               K I PK+AS++KLI++GKILEN KTV QCK PF +    V
Sbjct: 61  --------------------KKIVPKSASDIKLINAGKILENGKTVAQCKAPFDDLPKSV 99

Query: 121 TIMHVVVQPSLAKAK 136
             MHVVVQ S  KA+
Sbjct: 121 ITMHVVVQLSPTKAR 99

[Close]

BLAST of Carg24551 vs. ExPASy Swiss-Prot
Match: Q7XRU4 (Membrane-anchored ubiquitin-fold protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=MUB4 PE=2 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 1.7e-23
Identity = 58/141 (41.13%), Postives = 79/141 (56.03%), Query Frame = 0


Query: 4   DDLIDIKFRLYDGSDIGPFRYSS-ASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSAFV 63
           D+ +++KFRL+DGSDIGP R ++ A+T+  LK R+V+DWPK+                  
Sbjct: 20  DEEVEVKFRLFDGSDIGPLRCNAVATTVAALKDRVVADWPKD------------------ 79

Query: 64  FILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGVTI 123
                             KTI PK A++VKLIS GKILEN+K + QC+ PFG+       
Sbjct: 80  ------------------KTIVPKTANDVKLISGGKILENDKNIAQCRAPFGDLPSTAIT 124

Query: 124 MHVVVQPSLAKAKTGEARSSL 144
           MHVVVQPS AK+K  +  + L
Sbjct: 140 MHVVVQPSSAKSKPDKKTNKL 124

[Close]

BLAST of Carg24551 vs. ExPASy Swiss-Prot
Match: Q8LCS8 (Membrane-anchored ubiquitin-fold protein 2 OS=Arabidopsis thaliana OX=3702 GN=MUB2 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 3.3e-19
Identity = 49/124 (39.52%), Postives = 66/124 (53.23%), Query Frame = 0


Query: 5   DLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSAFVFI 64
           D ++IKFRL DGSDIGP  +  A+T+  LK+ +V+ WP++                    
Sbjct: 6   DQLEIKFRLNDGSDIGPKLFPDATTVATLKETVVAQWPRD-------------------- 65

Query: 65  LILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGVTIMH 124
                           K   PK   +VKLIS+G+ILENNKTVG C+ P G F+G VT MH
Sbjct: 66  ----------------KENGPKTVKDVKLISAGRILENNKTVGDCRSPVGNFSGAVTTMH 93

Query: 125 VVVQ 129
           V++Q
Sbjct: 126 VIIQ 93

[Close]

BLAST of Carg24551 vs. ExPASy Swiss-Prot
Match: Q6Z8K4 (Membrane-anchored ubiquitin-fold protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=MUB3 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.1e-18
Identity = 45/123 (36.59%), Postives = 70/123 (56.91%), Query Frame = 0


Query: 7   IDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSAFVFILI 66
           I++KFRL+DG+DIGP +Y  ++T+  LK+ I++ WP++                      
Sbjct: 8   IEVKFRLFDGTDIGPSKYDPSTTVSALKEFILARWPQD---------------------- 67

Query: 67  LLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGVTIMHVV 126
                         K ITPK  +++KLI++G+ILENN+T+ + ++P GE  GGV  MHVV
Sbjct: 68  --------------KEITPKTVNDLKLINAGRILENNRTLAESRVPVGEVPGGVITMHVV 94

Query: 127 VQP 130
           V+P
Sbjct: 128 VRP 94

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BLAST of Carg24551 vs. ExPASy TrEMBL
Match: A0A5A7UPG7 (Membrane-anchored ubiquitin-fold protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00340 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 3.6e-45
Identity = 101/138 (73.19%), Postives = 102/138 (73.91%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK                 
Sbjct: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK----------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                              GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV
Sbjct: 61  -------------------GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 102

Query: 121 TIMHVVVQPSLAKAKTGE 139
           TIMHVVVQPSLAKAKTG+
Sbjct: 121 TIMHVVVQPSLAKAKTGD 102

[Close]

BLAST of Carg24551 vs. ExPASy TrEMBL
Match: A0A6J1IG02 (Membrane-anchored ubiquitin-fold protein OS=Cucurbita maxima OX=3661 GN=LOC111472981 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 3.1e-44
Identity = 100/136 (73.53%), Postives = 100/136 (73.53%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK                 
Sbjct: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK----------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                              GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV
Sbjct: 61  -------------------GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 100

Query: 121 TIMHVVVQPSLAKAKT 137
           TIMHVVVQPSLAKAKT
Sbjct: 121 TIMHVVVQPSLAKAKT 100

[Close]

BLAST of Carg24551 vs. ExPASy TrEMBL
Match: A0A1S3CMX7 (Membrane-anchored ubiquitin-fold protein OS=Cucumis melo OX=3656 GN=LOC103502298 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 3.1e-44
Identity = 100/136 (73.53%), Postives = 100/136 (73.53%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK                 
Sbjct: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK----------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                              GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV
Sbjct: 61  -------------------GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 100

Query: 121 TIMHVVVQPSLAKAKT 137
           TIMHVVVQPSLAKAKT
Sbjct: 121 TIMHVVVQPSLAKAKT 100

[Close]

BLAST of Carg24551 vs. ExPASy TrEMBL
Match: A0A6J1EXB1 (Membrane-anchored ubiquitin-fold protein OS=Cucurbita moschata OX=3662 GN=LOC111439129 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 3.1e-44
Identity = 100/136 (73.53%), Postives = 100/136 (73.53%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK                 
Sbjct: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK----------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                              GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV
Sbjct: 61  -------------------GKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 100

Query: 121 TIMHVVVQPSLAKAKT 137
           TIMHVVVQPSLAKAKT
Sbjct: 121 TIMHVVVQPSLAKAKT 100

[Close]

BLAST of Carg24551 vs. ExPASy TrEMBL
Match: A0A0A0LNH9 (Membrane-anchored ubiquitin-fold protein OS=Cucumis sativus OX=3659 GN=Csa_1G001320 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 4.0e-44
Identity = 99/136 (72.79%), Postives = 100/136 (73.53%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK                 
Sbjct: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPK----------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                              GKTITPKAASE+KLISSGKILENNKTVGQCKLPFGEFTGGV
Sbjct: 61  -------------------GKTITPKAASEIKLISSGKILENNKTVGQCKLPFGEFTGGV 100

Query: 121 TIMHVVVQPSLAKAKT 137
           TIMHVVVQPSLAKAKT
Sbjct: 121 TIMHVVVQPSLAKAKT 100

[Close]

BLAST of Carg24551 vs. TAIR 10
Match: AT4G24990.1 (Ubiquitin family protein )

HSP 1 Score: 160.6 bits (405), Expect = 1.0e-39
Identity = 81/136 (59.56%), Postives = 91/136 (66.91%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPE++ IDIKFRLYDGSDIGPFRYS+AST+D LKQR+VSDWPK                 
Sbjct: 1   MPEEESIDIKFRLYDGSDIGPFRYSAASTVDFLKQRVVSDWPK----------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                              GKT+ PK  +EVKLISSGKILENNKTVGQCK PFG+  GGV
Sbjct: 61  -------------------GKTVVPKGINEVKLISSGKILENNKTVGQCKTPFGDIAGGV 100

Query: 121 TIMHVVVQPSLAKAKT 137
            +MHVVVQPSLAK+KT
Sbjct: 121 IVMHVVVQPSLAKSKT 100

[Close]

BLAST of Carg24551 vs. TAIR 10
Match: AT3G26980.1 (membrane-anchored ubiquitin-fold protein 4 precursor )

HSP 1 Score: 124.0 bits (310), Expect = 1.0e-28
Identity = 62/135 (45.93%), Postives = 81/135 (60.00%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           MPE+DL+++KFRLYDGSD+GPF+YS  +T+ +LK+RIVS+WPK+                
Sbjct: 1   MPEEDLVELKFRLYDGSDVGPFQYSPTATVSMLKERIVSEWPKD---------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGV 120
                               K I PK+AS++KLI++GKILEN KTV QCK PF +    V
Sbjct: 61  --------------------KKIVPKSASDIKLINAGKILENGKTVAQCKAPFDDLPKSV 99

Query: 121 TIMHVVVQPSLAKAK 136
             MHVVVQ S  KA+
Sbjct: 121 ITMHVVVQLSPTKAR 99

[Close]

BLAST of Carg24551 vs. TAIR 10
Match: AT5G15460.1 (membrane-anchored ubiquitin-fold protein 2 )

HSP 1 Score: 96.3 bits (238), Expect = 2.3e-20
Identity = 49/124 (39.52%), Postives = 66/124 (53.23%), Query Frame = 0


Query: 5   DLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSAFVFI 64
           D ++IKFRL DGSDIGP  +  A+T+  LK+ +V+ WP++                    
Sbjct: 6   DQLEIKFRLNDGSDIGPKLFPDATTVATLKETVVAQWPRD-------------------- 65

Query: 65  LILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGVTIMH 124
                           K   PK   +VKLIS+G+ILENNKTVG C+ P G F+G VT MH
Sbjct: 66  ----------------KENGPKTVKDVKLISAGRILENNKTVGDCRSPVGNFSGAVTTMH 93

Query: 125 VVVQ 129
           V++Q
Sbjct: 126 VIIQ 93

[Close]

BLAST of Carg24551 vs. TAIR 10
Match: AT5G15460.2 (membrane-anchored ubiquitin-fold protein 2 )

HSP 1 Score: 96.3 bits (238), Expect = 2.3e-20
Identity = 49/124 (39.52%), Postives = 66/124 (53.23%), Query Frame = 0


Query: 5   DLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSAFVFI 64
           D ++IKFRL DGSDIGP  +  A+T+  LK+ +V+ WP++                    
Sbjct: 6   DQLEIKFRLNDGSDIGPKLFPDATTVATLKETVVAQWPRD-------------------- 65

Query: 65  LILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCKLPFGEFTGGVTIMH 124
                           K   PK   +VKLIS+G+ILENNKTVG C+ P G F+G VT MH
Sbjct: 66  ----------------KENGPKTVKDVKLISAGRILENNKTVGDCRSPVGNFSGAVTTMH 93

Query: 125 VVVQ 129
           V++Q
Sbjct: 126 VIIQ 93

[Close]

BLAST of Carg24551 vs. TAIR 10
Match: AT1G77870.1 (membrane-anchored ubiquitin-fold protein 5 precursor )

HSP 1 Score: 93.6 bits (231), Expect = 1.5e-19
Identity = 52/137 (37.96%), Postives = 73/137 (53.28%), Query Frame = 0


Query: 1   MPEDDLIDIKFRLYDGSDIGPFRYSSASTIDVLKQRIVSDWPKEVSFFHWASDLLLFSSA 60
           M ++DLI++KFRL DG+DIGP +YS   T+  LK++I++ WPK+                
Sbjct: 1   MGDEDLIELKFRLADGTDIGPSKYSQFMTVASLKEKIIAQWPKD---------------- 60

Query: 61  FVFILILLFPFGTIFLYLLGKTITPKAASEVKLISSGKILENNKTVGQCK--LPFGEFTG 120
                               K   PK  +EVKLI+ GKILENNKT+ + +  +  GE  G
Sbjct: 61  --------------------KENAPKMINEVKLINGGKILENNKTLSEARSLITIGELPG 101

Query: 121 GVTIMHVVVQPSLAKAK 136
            VT MHVV++P L + K
Sbjct: 121 IVTTMHVVLRPPLFEKK 101

The following BLAST results are available for this feature:

BLAST of Carg24551 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021381.1	2.1e-79	100.00	Membrane-anchored ubiquitin-fold protein 3 [Cucurbita argyrosperma subsp. argyro...	[more]
KAA0057753.1	7.4e-45	73.19	membrane-anchored ubiquitin-fold protein 3 [Cucumis melo var. makuwa] >TYJ98433....	[more]
XP_008464397.1	6.3e-44	73.53	PREDICTED: membrane-anchored ubiquitin-fold protein 3 [Cucumis melo] >XP_0084643...	[more]
XP_031745197.1	8.2e-44	72.79	membrane-anchored ubiquitin-fold protein 3 [Cucumis sativus] >XP_031745199.1 mem...	[more]
XP_022134657.1	4.1e-43	72.06	membrane-anchored ubiquitin-fold protein 3-like [Momordica charantia] >XP_022134...	[more]

BLAST of Carg24551 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SW27	1.4e-38	59.56	Membrane-anchored ubiquitin-fold protein 3 OS=Arabidopsis thaliana OX=3702 GN=MU...	[more]
Q9LSD8	1.5e-27	45.93	Membrane-anchored ubiquitin-fold protein 4 OS=Arabidopsis thaliana OX=3702 GN=MU...	[more]
Q7XRU4	1.7e-23	41.13	Membrane-anchored ubiquitin-fold protein 4 OS=Oryza sativa subsp. japonica OX=39...	[more]
Q8LCS8	3.3e-19	39.52	Membrane-anchored ubiquitin-fold protein 2 OS=Arabidopsis thaliana OX=3702 GN=MU...	[more]
Q6Z8K4	2.1e-18	36.59	Membrane-anchored ubiquitin-fold protein 3 OS=Oryza sativa subsp. japonica OX=39...	[more]

BLAST of Carg24551 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UPG7	3.6e-45	73.19	Membrane-anchored ubiquitin-fold protein OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A6J1IG02	3.1e-44	73.53	Membrane-anchored ubiquitin-fold protein OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A1S3CMX7	3.1e-44	73.53	Membrane-anchored ubiquitin-fold protein OS=Cucumis melo OX=3656 GN=LOC103502298...	[more]
A0A6J1EXB1	3.1e-44	73.53	Membrane-anchored ubiquitin-fold protein OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A0A0LNH9	4.0e-44	72.79	Membrane-anchored ubiquitin-fold protein OS=Cucumis sativus OX=3659 GN=Csa_1G001...	[more]

BLAST of Carg24551 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24990.1	1.0e-39	59.56	Ubiquitin family protein	[more]
AT3G26980.1	1.0e-28	45.93	membrane-anchored ubiquitin-fold protein 4 precursor	[more]
AT5G15460.1	2.3e-20	39.52	membrane-anchored ubiquitin-fold protein 2	[more]
AT5G15460.2	2.3e-20	39.52	membrane-anchored ubiquitin-fold protein 2	[more]
AT1G77870.1	1.5e-19	37.96	membrane-anchored ubiquitin-fold protein 5 precursor	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017000	Membrane-anchored ubiquitin-fold protein	PIRSF	PIRSF032572	MUB	coord: 1..53 e-value: 9.4E-15 score: 52.5 coord: 79..147 e-value: 5.6E-21 score: 72.6
IPR039540	UBL3-like, ubiquitin domain	PFAM	PF13881	Rad60-SLD_2	coord: 6..46 e-value: 3.4E-12 score: 46.4 coord: 81..139 e-value: 2.0E-18 score: 66.5
None	No IPR available	GENE3D	3.10.20.90		coord: 1..136 e-value: 1.7E-38 score: 132.7
None	No IPR available	PANTHER	PTHR13169:SF3	MEMBRANE-ANCHORED UBIQUITIN-FOLD PROTEIN 3	coord: 1..47
None	No IPR available	PANTHER	PTHR13169:SF3	MEMBRANE-ANCHORED UBIQUITIN-FOLD PROTEIN 3	coord: 80..138
None	No IPR available	CDD	cd01814	Ubl_MUBs_plant	coord: 6..129 e-value: 4.04855E-38 score: 123.258
IPR040015	UBL3-like	PANTHER	PTHR13169	UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN	coord: 80..138
IPR040015	UBL3-like	PANTHER	PTHR13169	UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN	coord: 1..47
IPR029071	Ubiquitin-like domain superfamily	SUPERFAMILY	54236	Ubiquitin-like	coord: 2..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg24551-RA	Carg24551-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane