Carg25776 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25776
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRAN GTPase-activating protein 1-like
LocationCarg_Chr08: 1762377 .. 1763996 (-)
RNA-Seq ExpressionCarg25776
SyntenyCarg25776
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTGCAGCCCAGACATTCCCACCTCGTGTGATGTCAATTAAATTATGGCCTCCAAGCCAGAGCACTAGGTTCATGCTCATAGAACGAATCATCAAGAATCTTACAACACCCTCCATTTTTTCCCGGAAGTATGGCTTATTGAGCAAAGAAGAAGCAGAGGAGGATGCCAAGAAAGTAGAAGATATTGCTTTTGCAACTGCAAACCAACACTTTGAGAAGGAGCCAGATGGCGATGGGAGCTCTGCAGTTCAAATTTATGCCAAAGAATCTAGTAGGCTTATGCTGGATATATTGAAAAGAGGTCCTGTATTAAAGGAGGATGGAGAGGCAATAATATCTGAGAGGGCAGCTGCCAAAGGAACTGTTTTTGATATATCAGGAGGACGAAGGGCCTTTATTGATGCAGAGGAGGCTAAGGAGCTTTTAGAACCCCTAAAAGATCCTGGAAACCTGTTTACAAAGATCTGCTTTAGTAATAGAAGTTTTGGCTTAGATGCTGCTCGTGTTGCTGAACCGATCTTGATGTCCATCAAAGATAGGTTGACTGAAGTTGACCTATCAGACTTCATTGCTGGAAGGCCGGAAGGCGAGGCTCTTGAAGTCGTGAATATTTTTTCTTCAGCATTAGAAGGTTGTGACTTGAGGTCTCTGGACCTCTCAAATAATGCCATGGGTGAGAAGGGTGTCCGGGCATTTGGGTCCCTTTTGAAATCACAGAAACATTTGGAGGAGCTTTATTTGATGAATGATGGTATTTCGGAAGAAGCTGCAAGAGCAATCTGTGAGTTGATTCCGTCTACAGACAAGCTTCGTATCCTTCAGTTCCATAATAACATGACAGGCGATGAAGGTGCAATTGCAATTTCCGAAATCGTCAAGGGTTCTCCTGCATTGGAGGATTTCCGTTGTTCCTCGACCAGGGTAGGTTGCGAAGGAGGAGTGGCTCTAGCTGAAGCTATTGGGACTTGTACCCATCTCAAGAAGCTTGATTTGCGCGACAACATGTTCGGGGTGGAAGCTGGAATTGCTTTGAGTAAATCTATATCTGCTTTTCCAGGTCTTACTGAAATTTATCTTAGTTATTTGAACTTGGAGGATGAAGGAGCTGAAGCATTGGCTAACGCCCTCAAAGATTCTGCTCCTTCCCTTGAAGTTCTTGAAATGGCTGGAAATGATATAACTGCAAAAGGTGCTGCCTCAATTGCAGCCTGTGTAGCAACAAAGCAATTCCTTAGTAAGTTATATTTGGCTGAGAATGAACTGAAGGATGATGGTGTGATTTTGATTAGCAAGGCACTGCAGGATGGACACGGTCAGTTGAGCGAAGTCGACGTGAGCACAAATTCAATTCGAAGGGCCGGGGCAAGATTCTTAGCTCAAATATTAGTGCAGAAGCCAGGATTTAAGTTGCTAAACATCAATGGCAATTATATATCTGAAGAAGGGATCGACGAAGTGAAGGACATATTTAAGAATTCTCCCAGCATGCTTGGACCTCTGGATGAGAACGATCCAGAAGGAGAAGACTACGATGAAGATGCTGAAGAGAACGGCGATCACGACGATGAACTCGAATCGAAACTCAAGGGCCTTGACATTAAACGAGATGAGTAA

mRNA sequence

ATGGATTCTGCAGCCCAGACATTCCCACCTCGTGTGATGTCAATTAAATTATGGCCTCCAAGCCAGAGCACTAGGTTCATGCTCATAGAACGAATCATCAAGAATCTTACAACACCCTCCATTTTTTCCCGGAAGTATGGCTTATTGAGCAAAGAAGAAGCAGAGGAGGATGCCAAGAAAGTAGAAGATATTGCTTTTGCAACTGCAAACCAACACTTTGAGAAGGAGCCAGATGGCGATGGGAGCTCTGCAGTTCAAATTTATGCCAAAGAATCTAGTAGGCTTATGCTGGATATATTGAAAAGAGGTCCTGTATTAAAGGAGGATGGAGAGGCAATAATATCTGAGAGGGCAGCTGCCAAAGGAACTGTTTTTGATATATCAGGAGGACGAAGGGCCTTTATTGATGCAGAGGAGGCTAAGGAGCTTTTAGAACCCCTAAAAGATCCTGGAAACCTGTTTACAAAGATCTGCTTTAGTAATAGAAGTTTTGGCTTAGATGCTGCTCGTGTTGCTGAACCGATCTTGATGTCCATCAAAGATAGGTTGACTGAAGTTGACCTATCAGACTTCATTGCTGGAAGGCCGGAAGGCGAGGCTCTTGAAGTCGTGAATATTTTTTCTTCAGCATTAGAAGGTTGTGACTTGAGGTCTCTGGACCTCTCAAATAATGCCATGGGTGAGAAGGGTGTCCGGGCATTTGGGTCCCTTTTGAAATCACAGAAACATTTGGAGGAGCTTTATTTGATGAATGATGGTATTTCGGAAGAAGCTGCAAGAGCAATCTGTGAGTTGATTCCGTCTACAGACAAGCTTCGTATCCTTCAGTTCCATAATAACATGACAGGCGATGAAGGTGCAATTGCAATTTCCGAAATCGTCAAGGGTTCTCCTGCATTGGAGGATTTCCGTTGTTCCTCGACCAGGGTAGGTTGCGAAGGAGGAGTGGCTCTAGCTGAAGCTATTGGGACTTGTACCCATCTCAAGAAGCTTGATTTGCGCGACAACATGTTCGGGGTGGAAGCTGGAATTGCTTTGAGTAAATCTATATCTGCTTTTCCAGGTCTTACTGAAATTTATCTTAGTTATTTGAACTTGGAGGATGAAGGAGCTGAAGCATTGGCTAACGCCCTCAAAGATTCTGCTCCTTCCCTTGAAGTTCTTGAAATGGCTGGAAATGATATAACTGCAAAAGGTGCTGCCTCAATTGCAGCCTGTGTAGCAACAAAGCAATTCCTTAGTAAGTTATATTTGGCTGAGAATGAACTGAAGGATGATGGTGTGATTTTGATTAGCAAGGCACTGCAGGATGGACACGGTCAGTTGAGCGAAGTCGACGTGAGCACAAATTCAATTCGAAGGGCCGGGGCAAGATTCTTAGCTCAAATATTAGTGCAGAAGCCAGGATTTAAGTTGCTAAACATCAATGGCAATTATATATCTGAAGAAGGGATCGACGAAGTGAAGGACATATTTAAGAATTCTCCCAGCATGCTTGGACCTCTGGATGAGAACGATCCAGAAGGAGAAGACTACGATGAAGATGCTGAAGAGAACGGCGATCACGACGATGAACTCGAATCGAAACTCAAGGGCCTTGACATTAAACGAGATGAGTAA

Coding sequence (CDS)

ATGGATTCTGCAGCCCAGACATTCCCACCTCGTGTGATGTCAATTAAATTATGGCCTCCAAGCCAGAGCACTAGGTTCATGCTCATAGAACGAATCATCAAGAATCTTACAACACCCTCCATTTTTTCCCGGAAGTATGGCTTATTGAGCAAAGAAGAAGCAGAGGAGGATGCCAAGAAAGTAGAAGATATTGCTTTTGCAACTGCAAACCAACACTTTGAGAAGGAGCCAGATGGCGATGGGAGCTCTGCAGTTCAAATTTATGCCAAAGAATCTAGTAGGCTTATGCTGGATATATTGAAAAGAGGTCCTGTATTAAAGGAGGATGGAGAGGCAATAATATCTGAGAGGGCAGCTGCCAAAGGAACTGTTTTTGATATATCAGGAGGACGAAGGGCCTTTATTGATGCAGAGGAGGCTAAGGAGCTTTTAGAACCCCTAAAAGATCCTGGAAACCTGTTTACAAAGATCTGCTTTAGTAATAGAAGTTTTGGCTTAGATGCTGCTCGTGTTGCTGAACCGATCTTGATGTCCATCAAAGATAGGTTGACTGAAGTTGACCTATCAGACTTCATTGCTGGAAGGCCGGAAGGCGAGGCTCTTGAAGTCGTGAATATTTTTTCTTCAGCATTAGAAGGTTGTGACTTGAGGTCTCTGGACCTCTCAAATAATGCCATGGGTGAGAAGGGTGTCCGGGCATTTGGGTCCCTTTTGAAATCACAGAAACATTTGGAGGAGCTTTATTTGATGAATGATGGTATTTCGGAAGAAGCTGCAAGAGCAATCTGTGAGTTGATTCCGTCTACAGACAAGCTTCGTATCCTTCAGTTCCATAATAACATGACAGGCGATGAAGGTGCAATTGCAATTTCCGAAATCGTCAAGGGTTCTCCTGCATTGGAGGATTTCCGTTGTTCCTCGACCAGGGTAGGTTGCGAAGGAGGAGTGGCTCTAGCTGAAGCTATTGGGACTTGTACCCATCTCAAGAAGCTTGATTTGCGCGACAACATGTTCGGGGTGGAAGCTGGAATTGCTTTGAGTAAATCTATATCTGCTTTTCCAGGTCTTACTGAAATTTATCTTAGTTATTTGAACTTGGAGGATGAAGGAGCTGAAGCATTGGCTAACGCCCTCAAAGATTCTGCTCCTTCCCTTGAAGTTCTTGAAATGGCTGGAAATGATATAACTGCAAAAGGTGCTGCCTCAATTGCAGCCTGTGTAGCAACAAAGCAATTCCTTAGTAAGTTATATTTGGCTGAGAATGAACTGAAGGATGATGGTGTGATTTTGATTAGCAAGGCACTGCAGGATGGACACGGTCAGTTGAGCGAAGTCGACGTGAGCACAAATTCAATTCGAAGGGCCGGGGCAAGATTCTTAGCTCAAATATTAGTGCAGAAGCCAGGATTTAAGTTGCTAAACATCAATGGCAATTATATATCTGAAGAAGGGATCGACGAAGTGAAGGACATATTTAAGAATTCTCCCAGCATGCTTGGACCTCTGGATGAGAACGATCCAGAAGGAGAAGACTACGATGAAGATGCTGAAGAGAACGGCGATCACGACGATGAACTCGAATCGAAACTCAAGGGCCTTGACATTAAACGAGATGAGTAA

Protein sequence

MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKKVEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAAKGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIKDRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKSQKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPALEDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIYLSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAENELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYISEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE
Homology
BLAST of Carg25776 vs. NCBI nr
Match: KAG7025494.1 (RAN GTPase-activating protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1040.0 bits (2688), Expect = 7.0e-300
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK
Sbjct: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA
Sbjct: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
           KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK
Sbjct: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS
Sbjct: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL
Sbjct: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY
Sbjct: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE
Sbjct: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI
Sbjct: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE
Sbjct: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 539

BLAST of Carg25776 vs. NCBI nr
Match: KAG6593086.1 (RAN GTPase-activating protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1034.6 bits (2674), Expect = 2.9e-298
Identity = 536/539 (99.44%), Postives = 538/539 (99.81%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK
Sbjct: 24  MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 83

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA
Sbjct: 84  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 143

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
           KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVA+PILMSIK
Sbjct: 144 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAQPILMSIK 203

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS
Sbjct: 204 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 263

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL
Sbjct: 264 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 323

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTRVG EGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKS+SAFPGLTEIY
Sbjct: 324 EDFRCSSTRVGSEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSVSAFPGLTEIY 383

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE
Sbjct: 384 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 443

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI
Sbjct: 444 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 503

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE
Sbjct: 504 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 562

BLAST of Carg25776 vs. NCBI nr
Match: XP_022960216.1 (RAN GTPase-activating protein 1-like [Cucurbita moschata])

HSP 1 Score: 1034.2 bits (2673), Expect = 3.8e-298
Identity = 537/539 (99.63%), Postives = 537/539 (99.63%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK
Sbjct: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA
Sbjct: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
           KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK
Sbjct: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS
Sbjct: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL
Sbjct: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTRVG EGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY
Sbjct: 301 EDFRCSSTRVGSEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE
Sbjct: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKDDGVILISKALQDGHGQLSEVDVS NSIRRAGARFLAQILVQKPGFKLLNINGNYI
Sbjct: 421 NELKDDGVILISKALQDGHGQLSEVDVSANSIRRAGARFLAQILVQKPGFKLLNINGNYI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE
Sbjct: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 539

BLAST of Carg25776 vs. NCBI nr
Match: XP_023004254.1 (RAN GTPase-activating protein 1-like [Cucurbita maxima] >XP_023514749.1 RAN GTPase-activating protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1033.9 bits (2672), Expect = 5.0e-298
Identity = 537/539 (99.63%), Postives = 537/539 (99.63%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK
Sbjct: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA
Sbjct: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
           KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK
Sbjct: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS
Sbjct: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL
Sbjct: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTRVG EGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY
Sbjct: 301 EDFRCSSTRVGSEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE
Sbjct: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNIN NYI
Sbjct: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINANYI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE
Sbjct: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 539

BLAST of Carg25776 vs. NCBI nr
Match: XP_038898694.1 (RAN GTPase-activating protein 1 [Benincasa hispida])

HSP 1 Score: 981.5 bits (2536), Expect = 3.0e-282
Identity = 502/539 (93.14%), Postives = 526/539 (97.59%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MDS+ QTF PRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKE+AEEDAK+
Sbjct: 1   MDSSTQTFQPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEDAEEDAKQ 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           VED+AFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGP +KEDGE +ISE+A  
Sbjct: 61  VEDMAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPRVKEDGEVLISEKATT 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
           +GTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPIL+SIK
Sbjct: 121 RGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILLSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           DRLTEVDLSDFIAGR EGEAL+V+NIFS+ALEGCDLR LDLSNNAMGEKGVRAFGSLL+S
Sbjct: 181 DRLTEVDLSDFIAGRSEGEALQVMNIFSAALEGCDLRYLDLSNNAMGEKGVRAFGSLLRS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           QK+LEELYLMNDGISEEAARA+CELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL
Sbjct: 241 QKNLEELYLMNDGISEEAARAVCELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTRVG EGGVALAEAIG CT LKKLDLRDNMFGVEAG+ALSKSISAFPGLTEIY
Sbjct: 301 EDFRCSSTRVGSEGGVALAEAIGACTCLKKLDLRDNMFGVEAGMALSKSISAFPGLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           LSYLNLEDEGAEALANALKDSAPSL+VLEMAGNDITAKGAASIAACVATKQFLSKLYLAE
Sbjct: 361 LSYLNLEDEGAEALANALKDSAPSLDVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKDDGVILI KALQDGHGQLSEVD+STNS+RRAGARF+AQILVQKPGFKLLNIN NYI
Sbjct: 421 NELKDDGVILIGKALQDGHGQLSEVDISTNSVRRAGARFIAQILVQKPGFKLLNINANYI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEVK+IFKNSP+MLGPLDENDP+GEDYDEDAEENGDHDDELESKLKGLDIKR+E
Sbjct: 481 SEEGIDEVKEIFKNSPNMLGPLDENDPDGEDYDEDAEENGDHDDELESKLKGLDIKREE 539

BLAST of Carg25776 vs. ExPASy Swiss-Prot
Match: Q9LE82 (RAN GTPase-activating protein 1 OS=Arabidopsis thaliana OX=3702 GN=RANGAP1 PE=1 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 4.2e-207
Identity = 374/540 (69.26%), Postives = 452/540 (83.70%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MD +A+T   RV+S+K+WPPS+STR ML+ER+ KN+TTPSIFSRKYGLLS EEAE+DAK+
Sbjct: 1   MDHSAKTTQNRVLSVKMWPPSKSTRLMLVERMTKNITTPSIFSRKYGLLSVEEAEQDAKR 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           +ED+AFATAN+HF+ EPDGDG+SAV +YAKESS+LMLD++KRGP  +E+ E  +S+    
Sbjct: 61  IEDLAFATANKHFQNEPDGDGTSAVHVYAKESSKLMLDVIKRGP--QEESEVEVSKDG-- 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
               FDISGG RAFI+ EEA++LL PL DP N +TKI FSNRSFG +AA+ A  +L SIK
Sbjct: 121 -DVFFDISGGSRAFIEEEEARDLLRPLADPRNSYTKIRFSNRSFGSEAAKFAASVLSSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           D+LTEVDLSDF+AGRPE EALEV+N+FSSALEG  LR L+LS+NA+GEKG+RAF SL+ S
Sbjct: 181 DQLTEVDLSDFVAGRPEAEALEVMNMFSSALEGSKLRYLNLSDNALGEKGIRAFASLINS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           Q  LEELYLMNDGISE+AARA+ EL+PSTDK+R+LQFHNNMTGDEGA AI+EIV+  P+L
Sbjct: 241 QHDLEELYLMNDGISEDAARAVRELLPSTDKIRVLQFHNNMTGDEGATAIAEIVRECPSL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTR+G EGGVALAEA+  C+HLKKLDLRDNMFGVE GIAL+K++S    LTEIY
Sbjct: 301 EDFRCSSTRIGSEGGVALAEALEHCSHLKKLDLRDNMFGVEGGIALAKTLSVLTHLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           +SYLNLEDEG EAL+ AL  SAPSLEVLE+AGNDIT K   ++AAC+A+KQ L+KL L+E
Sbjct: 361 MSYLNLEDEGTEALSEALLKSAPSLEVLELAGNDITVKSTGNLAACIASKQSLAKLNLSE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKD+G ILI+KA+ +GH QL EVD+STN IRRAGAR LAQ +V+K  FKLLNINGN+I
Sbjct: 421 NELKDEGTILIAKAV-EGHDQLVEVDLSTNMIRRAGARALAQTVVKKNTFKLLNINGNFI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDY-DEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEV D+FK+    L PLD+NDPEGED+ DED EE G+  +ELESKL  L IK+ E
Sbjct: 481 SEEGIDEVNDMFKDCLDKLVPLDDNDPEGEDFEDEDEEEEGEDGNELESKLGSLKIKQGE 534

BLAST of Carg25776 vs. ExPASy Swiss-Prot
Match: Q9M651 (RAN GTPase-activating protein 2 OS=Arabidopsis thaliana OX=3702 GN=RANGAP2 PE=1 SV=2)

HSP 1 Score: 696.4 bits (1796), Expect = 2.5e-199
Identity = 352/537 (65.55%), Postives = 447/537 (83.24%), Query Frame = 0

Query: 10  PRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKKVEDIAFATA 69
           P   SIKLWPPS  TR  LIERI  N ++ +IF+ KYG L+K++A E+AK++EDIAF+TA
Sbjct: 9   PHAFSIKLWPPSLPTRKALIERITNNFSSKTIFTEKYGSLTKDQATENAKRIEDIAFSTA 68

Query: 70  NQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA-KGTVFDIS 129
           NQ FE+EPDGDG SAVQ+YAKE S+L+L++LK+GPV K     +ISE + + + T FDIS
Sbjct: 69  NQQFEREPDGDGGSAVQLYAKECSKLILEVLKKGPVAKVAARELISEDSVSPRETFFDIS 128

Query: 130 GGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIKDRLTEVDL 189
            G+RAFI+AEEA+ELL+PLK+PGN +TKICFSNRSFGL AARVAEPIL S+KD+L EVDL
Sbjct: 129 KGKRAFIEAEEAEELLKPLKEPGNAYTKICFSNRSFGLGAARVAEPILASLKDQLKEVDL 188

Query: 190 SDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKSQKHLEELY 249
           SDF+AGRPE EALEV+NIFS AL+G  L SL+LS+NA+GEKGVRAFG+LLKS   LEELY
Sbjct: 189 SDFVAGRPELEALEVMNIFSDALQGSILSSLNLSDNALGEKGVRAFGALLKSLSSLEELY 248

Query: 250 LMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPALEDFRCSST 309
           LMNDGIS+EAA+A+ ELIPST+ LR+L FHNNMTGDEGA+AI+E+VK SP LE+FRCSST
Sbjct: 249 LMNDGISKEAAQAVSELIPSTENLRVLHFHNNMTGDEGALAIAEVVKRSPLLENFRCSST 308

Query: 310 RVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIYLSYLNLED 369
           RVG +GG+AL+EA+  CTH++KLDLRDNMFG EAG++LSK++S+F  +TE+YLSYLNLED
Sbjct: 309 RVGSKGGIALSEALEHCTHMEKLDLRDNMFGTEAGVSLSKTLSSFKHMTELYLSYLNLED 368

Query: 370 EGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAENELKDDGV 429
           EGA A+ NALK+SA  +EVLEMAGNDIT + A++IAACVA KQ L+KL L+ENELKD+G 
Sbjct: 369 EGAIAIVNALKESASPIEVLEMAGNDITVEAASAIAACVAAKQDLNKLNLSENELKDEGC 428

Query: 430 ILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYISEEGIDEV 489
           + I+  +++GH +L  +D+STN IRRAGAR LA ++V+K  FKLLNI+GN ISEEGI+E+
Sbjct: 429 VQIANCIEEGHSKLQYIDMSTNYIRRAGARALAHVVVKKEAFKLLNIDGNIISEEGIEEL 488

Query: 490 KDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDD------ELESKLKGLDIKRDE 540
           K+IFK SP +LG LDENDP+GE+ D+D E+  D ++      ELESKLK L++ +++
Sbjct: 489 KEIFKKSPELLGALDENDPDGEEDDDDEEDEEDEENEGNGNGELESKLKNLEVNQED 545

BLAST of Carg25776 vs. ExPASy Swiss-Prot
Match: Q5DU56 (Protein NLRC3 OS=Mus musculus OX=10090 GN=Nlrc3 PE=1 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 2.8e-25
Identity = 97/376 (25.80%), Postives = 174/376 (46.28%), Query Frame = 0

Query: 89   AKESSRLMLDILKRGPVLKEDGEAIISERAAAKGTVFDISGGRRAFIDAEEAKELLEPLK 148
            A + +R +  +  +  V+K+DG   ++E   +  T+  +   ++  I    A+++ + LK
Sbjct: 716  ALKINRTLTSLSLQSNVIKDDGVMCVAEALVSNQTI-SMLQLQKNLIGLIGAQQMADALK 775

Query: 149  DPGNLFTKICFSNRSFGLDAARVAEPILMSIKDRLTEVDLSDFIAGRPEGEALEVVNIFS 208
               +L   + FS+ + G D   +A                          EAL+V  I  
Sbjct: 776  QNRSL-KALMFSSNTIG-DRGAIA------------------------LAEALKVNQI-- 835

Query: 209  SALEGCDLRSLDLSNNAMGEKGVRAFGSLLKSQKHLEELYLMNDGISEEAARAICELIPS 268
                   L +LDL +N++ + GV      L S + L  L L  + IS E A+A+ + +  
Sbjct: 836  -------LENLDLQSNSISDMGVTVLMRALCSNQTLSSLNLRENSISPEGAQALTQALCR 895

Query: 269  TDKLRILQFHNNMTGDEGAIAISEIVKGSPALEDFRCSSTRVGCEGGVALAEAIGTCTHL 328
             + L+ L    N+  D GA AI+  V  + +L         +      AL +A+     L
Sbjct: 896  NNTLKHLDLTANLLHDRGAQAIAVAVGENHSLTHLHLQWNFIQAGAARALGQALQLNRTL 955

Query: 329  KKLDLRDNMFGVEAGIALSKSISAFPGLTEIYLSYLNLEDEGAEALANALKDSAPSLEVL 388
              LDL++N  G E   +++ ++     L  +YL   ++  +GA+AL  AL  +  +LE+L
Sbjct: 956  TTLDLQENAIGDEGASSVAGALKVNTTLIALYLQVASIGSQGAQALGEALTVNR-TLEIL 1015

Query: 389  EMAGNDITAKGAASIAACVATKQFLSKLYLAENELKDDGVILISKALQDGHGQLSEVDVS 448
            ++ GND+ A GA ++A  +     L +L L EN L  DG I ++ AL + HG L  +++ 
Sbjct: 1016 DLRGNDVGAAGAKALANALKLNSSLRRLNLQENSLGMDGAIFVASALSENHG-LHHINLQ 1053

Query: 449  TNSIRRAGARFLAQIL 465
             N I  + AR +++ +
Sbjct: 1076 GNPIGESAARMISEAI 1053

BLAST of Carg25776 vs. ExPASy Swiss-Prot
Match: Q7RTR2 (NLR family CARD domain-containing protein 3 OS=Homo sapiens OX=9606 GN=NLRC3 PE=1 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 4.8e-25
Identity = 76/249 (30.52%), Postives = 126/249 (50.60%), Query Frame = 0

Query: 216  LRSLDLSNNAMGEKGVRAFGSLLKSQKHLEELYLMNDGISEEAARAICELIPSTDKLRIL 275
            L SLDL +N++ + GV A    L + + L  L L  + IS E A+AI   + +   L+ L
Sbjct: 808  LESLDLQSNSISDAGVAALMGALCTNQTLLSLSLRENSISPEGAQAIAHALCANSTLKNL 867

Query: 276  QFHNNMTGDEGAIAISEIVKGSPALEDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRD 335
                N+  D+GA AI+  V+ +  L         +      AL +A+     L  LDL++
Sbjct: 868  DLTANLLHDQGARAIAVAVRENRTLTSLHLQWNFIQAGAAQALGQALQLNRSLTSLDLQE 927

Query: 336  NMFGVEAGIALSKSISAFPGLTEIYLSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDI 395
            N  G +   A+++++     LT +YL   ++   GA+ L  AL  +  +LE+L++ GN I
Sbjct: 928  NAIGDDGACAVARALKVNTALTALYLQVASIGASGAQVLGEALAVNR-TLEILDLRGNAI 987

Query: 396  TAKGAASIAACVATKQFLSKLYLAENELKDDGVILISKALQDGHGQLSEVDVSTNSIRRA 455
               GA ++A  +     L +L L EN L  DG I I+ AL   H +L  +++  N I  +
Sbjct: 988  GVAGAKALANALKVNSSLRRLNLQENSLGMDGAICIATALSGNH-RLQHINLQGNHIGDS 1047

Query: 456  GARFLAQIL 465
            GAR +++ +
Sbjct: 1048 GARMISEAI 1054

BLAST of Carg25776 vs. ExPASy Swiss-Prot
Match: P46061 (Ran GTPase-activating protein 1 OS=Mus musculus OX=10090 GN=Rangap1 PE=1 SV=2)

HSP 1 Score: 113.2 bits (282), Expect = 9.0e-24
Identity = 108/397 (27.20%), Postives = 176/397 (44.33%), Query Frame = 0

Query: 137 AEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIKDRLTEVDLSDFIAGRP 196
           AE+AK++++ +++   L   +     + G++AARV    L   K  L     SD   GR 
Sbjct: 35  AEDAKDVIKEIEEFDGL-EALRLEGNTVGVEAARVIAKALEK-KSELKRCHWSDMFTGRL 94

Query: 197 EGEALEVVNIFSSAL--EGCDLRSLDLSNNAMGEKGVRAFGSLLKSQK--HLEELYLMND 256
             E    +      L   G  L  LDLS+NA G  GVR F +LLKS     L+EL L N 
Sbjct: 95  RSEIPPALISLGEGLITAGAQLVELDLSDNAFGPDGVRGFEALLKSPACFTLQELKLNNC 154

Query: 257 GISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPALEDFRCSSTRVGC 316
           G+     + +           + + H   +     +A+   V G   LE+          
Sbjct: 155 GMGIGGGKILA--------AALTECHRKSSAQGKPLALKVFVAGRNRLEN---------- 214

Query: 317 EGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIYLSYLNLEDEGAE 376
           +G  ALAEA G             + G                L E+++    +   G  
Sbjct: 215 DGATALAEAFG-------------IIGT---------------LEEVHMPQNGINHPGVT 274

Query: 377 ALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAENELKDDGVILIS 436
           ALA A   + P L V+ +  N  T KG  ++A  + T + +  +   +  ++  G + I+
Sbjct: 275 ALAQAFAIN-PLLRVINLNDNTFTEKGGVAMAETLKTLRQVEVINFGDCLVRSKGAVAIA 334

Query: 437 KALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYISEEGIDEVKDIF 496
            A++ G  +L E+++S   I+R  A  +A+ +  K   + L++NGN + EEG ++++++ 
Sbjct: 335 DAVRGGLPKLKELNLSFCEIKRDAALVVAEAVADKAELEKLDLNGNALGEEGCEQLQEVM 378

Query: 497 K--NSPSMLGPLDENDPEGEDYDEDAEENGDHDDELE 528
              N   +L  L  +D EGE  DED EE G+ DDE E
Sbjct: 395 DSFNMAKVLASL--SDDEGE--DEDEEEEGEEDDEEE 378

BLAST of Carg25776 vs. ExPASy TrEMBL
Match: A0A6J1H710 (RAN GTPase-activating protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111461021 PE=4 SV=1)

HSP 1 Score: 1034.2 bits (2673), Expect = 1.9e-298
Identity = 537/539 (99.63%), Postives = 537/539 (99.63%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK
Sbjct: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA
Sbjct: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
           KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK
Sbjct: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS
Sbjct: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL
Sbjct: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTRVG EGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY
Sbjct: 301 EDFRCSSTRVGSEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE
Sbjct: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKDDGVILISKALQDGHGQLSEVDVS NSIRRAGARFLAQILVQKPGFKLLNINGNYI
Sbjct: 421 NELKDDGVILISKALQDGHGQLSEVDVSANSIRRAGARFLAQILVQKPGFKLLNINGNYI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE
Sbjct: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 539

BLAST of Carg25776 vs. ExPASy TrEMBL
Match: A0A6J1KVR8 (RAN GTPase-activating protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497640 PE=4 SV=1)

HSP 1 Score: 1033.9 bits (2672), Expect = 2.4e-298
Identity = 537/539 (99.63%), Postives = 537/539 (99.63%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK
Sbjct: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA
Sbjct: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
           KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK
Sbjct: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS
Sbjct: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL
Sbjct: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTRVG EGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY
Sbjct: 301 EDFRCSSTRVGSEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE
Sbjct: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNIN NYI
Sbjct: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINANYI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE
Sbjct: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 539

BLAST of Carg25776 vs. ExPASy TrEMBL
Match: A0A5A7UUV6 (RAN GTPase-activating protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00610 PE=4 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 3.5e-281
Identity = 501/539 (92.95%), Postives = 525/539 (97.40%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MDS+ QTF PRVMSIKLWPPSQSTRFML+ERIIKNLTTPSIFSRKYGLLSKEEAEEDAK+
Sbjct: 1   MDSSTQTFQPRVMSIKLWPPSQSTRFMLVERIIKNLTTPSIFSRKYGLLSKEEAEEDAKQ 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           VED+AFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGP +KEDGE +ISE+ A 
Sbjct: 61  VEDMAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPRVKEDGEVLISEKPAT 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
           +GTVFDISGGRRAFIDA+EAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPIL+SIK
Sbjct: 121 RGTVFDISGGRRAFIDADEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILLSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           DRLTEVDLSDFIAGR EG+ALEV+NIFS+ALEGCDLR LDLSNNAMGEKGVRAFGSLL+S
Sbjct: 181 DRLTEVDLSDFIAGRSEGDALEVMNIFSAALEGCDLRYLDLSNNAMGEKGVRAFGSLLRS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           QK+LEELYLMNDGISEEAARA+ ELIPSTDKLRILQFHNNMTGDEGA+AISEIVKGSPAL
Sbjct: 241 QKNLEELYLMNDGISEEAARAVRELIPSTDKLRILQFHNNMTGDEGAVAISEIVKGSPAL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTRVG EGGVALAEAIGTCT LKKLDLRDNMFGVEAGIALSKSIS+FPGLTEIY
Sbjct: 301 EDFRCSSTRVGSEGGVALAEAIGTCTRLKKLDLRDNMFGVEAGIALSKSISSFPGLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           LSYLNLEDEGAEALANALKDSAPSLEVLE+AGNDITAKGAASIAACVATKQFLSKLYLAE
Sbjct: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEIAGNDITAKGAASIAACVATKQFLSKLYLAE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKDDGVILI KALQDGHGQLSEVD STNSIRRAGARF+AQILVQKPGFKLLNIN NYI
Sbjct: 421 NELKDDGVILIGKALQDGHGQLSEVDFSTNSIRRAGARFVAQILVQKPGFKLLNINSNYI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEVK+IFKNSP+MLGPLDENDP+GEDYDEDAEENGDHDDELESKLKGLDIKR+E
Sbjct: 481 SEEGIDEVKEIFKNSPNMLGPLDENDPDGEDYDEDAEENGDHDDELESKLKGLDIKREE 539

BLAST of Carg25776 vs. ExPASy TrEMBL
Match: A0A1S3BRW2 (RAN GTPase-activating protein 1 OS=Cucumis melo OX=3656 GN=LOC103492625 PE=4 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.3e-280
Identity = 500/539 (92.76%), Postives = 524/539 (97.22%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MDS+ QTF PRVMSIKLWPPSQSTRFML+ERIIKNLTTPSIFSRKYGLLSKEEAEEDAK+
Sbjct: 1   MDSSTQTFQPRVMSIKLWPPSQSTRFMLVERIIKNLTTPSIFSRKYGLLSKEEAEEDAKQ 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           VED+AFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGP +KEDGE +ISE+ A 
Sbjct: 61  VEDMAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPRVKEDGEVLISEKPAT 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
           +GTVFDISGGRRAFIDA+EAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPIL+SIK
Sbjct: 121 RGTVFDISGGRRAFIDADEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILLSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           DRLTEVDLSDFIAGR EG+ALEV+NIFS+ALEGCDLR LDLSNNAMGEKGVRAFGSLL+S
Sbjct: 181 DRLTEVDLSDFIAGRSEGDALEVMNIFSAALEGCDLRYLDLSNNAMGEKGVRAFGSLLRS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           QK+LEELYLMNDGISEEAARA+ ELIPSTDKLRILQFHNNMTGDEGA+AISEIVKGSPAL
Sbjct: 241 QKNLEELYLMNDGISEEAARAVRELIPSTDKLRILQFHNNMTGDEGAVAISEIVKGSPAL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTRVG EGGVALAEAIGTCT LKKLDLRDNMFGVEAGIALSKSIS+FPGLTEIY
Sbjct: 301 EDFRCSSTRVGSEGGVALAEAIGTCTRLKKLDLRDNMFGVEAGIALSKSISSFPGLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           LSYLNLEDEGAEALANALKDSAPSLEVLE+AGNDITAKGAASIAACVATKQFLSKLYLAE
Sbjct: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEIAGNDITAKGAASIAACVATKQFLSKLYLAE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKDDGVILI  ALQDGHGQLSEVD STNSIRRAGARF+AQILVQKPGFKLLNIN NYI
Sbjct: 421 NELKDDGVILIGNALQDGHGQLSEVDFSTNSIRRAGARFVAQILVQKPGFKLLNINSNYI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEVK+IFKNSP+MLGPLDENDP+GEDYDEDAEENGDHDDELESKLKGLDIKR+E
Sbjct: 481 SEEGIDEVKEIFKNSPNMLGPLDENDPDGEDYDEDAEENGDHDDELESKLKGLDIKREE 539

BLAST of Carg25776 vs. ExPASy TrEMBL
Match: A0A0A0K764 (WPP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378480 PE=4 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 9.9e-276
Identity = 492/539 (91.28%), Postives = 518/539 (96.10%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MDS+ QTF PRVMSIKLWPPSQSTRFML+ERIIKNLTTPSIFSRKYGLLSKEEAEEDAK+
Sbjct: 1   MDSSTQTFQPRVMSIKLWPPSQSTRFMLVERIIKNLTTPSIFSRKYGLLSKEEAEEDAKQ 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           VED+AF TANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGP +KEDGE +ISE++  
Sbjct: 61  VEDMAFVTANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPRVKEDGEVLISEKSTT 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
           +GTVFDISGGRRAFIDAEEA+ LLEPLKDPGNLFTKICFSNRSFGLDAARVAEPIL SIK
Sbjct: 121 RGTVFDISGGRRAFIDAEEAEVLLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILFSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           DRLTEVDLSDFIAGR EG+ALEV+NIFS+ALEGCDLR LDLSNNAMGEKGVRAFG LL+S
Sbjct: 181 DRLTEVDLSDFIAGRSEGDALEVMNIFSAALEGCDLRYLDLSNNAMGEKGVRAFGLLLRS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           QK+LEELYLMNDGISEEAARA+ ELIPSTDKLRILQFHNNMTGDEGAI+ISEIVK SPAL
Sbjct: 241 QKNLEELYLMNDGISEEAARAVRELIPSTDKLRILQFHNNMTGDEGAISISEIVKSSPAL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTRVG EGGVALAEAIGTCT LKKLDLRDNMFGVEAG+ALSKSIS+FPGLTEIY
Sbjct: 301 EDFRCSSTRVGSEGGVALAEAIGTCTRLKKLDLRDNMFGVEAGVALSKSISSFPGLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           LSYLNLEDEGAEALANALKDSAPSLEVLE+AGNDITAKGA SIAACVATKQFLSKLYLAE
Sbjct: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEVAGNDITAKGAVSIAACVATKQFLSKLYLAE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKDDGVILI KALQDGHGQLSEVD STNSIRRAGARF+AQILVQKPGFKLLNIN NYI
Sbjct: 421 NELKDDGVILIGKALQDGHGQLSEVDFSTNSIRRAGARFVAQILVQKPGFKLLNINANYI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEVK+IFKNSP+MLG LDENDP+GEDYDEDAEENGDHDDELESKLKGLDIK++E
Sbjct: 481 SEEGIDEVKEIFKNSPNMLGSLDENDPDGEDYDEDAEENGDHDDELESKLKGLDIKQEE 539

BLAST of Carg25776 vs. TAIR 10
Match: AT3G63130.1 (RAN GTPase activating protein 1 )

HSP 1 Score: 722.2 bits (1863), Expect = 3.0e-208
Identity = 374/540 (69.26%), Postives = 452/540 (83.70%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MD +A+T   RV+S+K+WPPS+STR ML+ER+ KN+TTPSIFSRKYGLLS EEAE+DAK+
Sbjct: 1   MDHSAKTTQNRVLSVKMWPPSKSTRLMLVERMTKNITTPSIFSRKYGLLSVEEAEQDAKR 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           +ED+AFATAN+HF+ EPDGDG+SAV +YAKESS+LMLD++KRGP  +E+ E  +S+    
Sbjct: 61  IEDLAFATANKHFQNEPDGDGTSAVHVYAKESSKLMLDVIKRGP--QEESEVEVSKDG-- 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
               FDISGG RAFI+ EEA++LL PL DP N +TKI FSNRSFG +AA+ A  +L SIK
Sbjct: 121 -DVFFDISGGSRAFIEEEEARDLLRPLADPRNSYTKIRFSNRSFGSEAAKFAASVLSSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           D+LTEVDLSDF+AGRPE EALEV+N+FSSALEG  LR L+LS+NA+GEKG+RAF SL+ S
Sbjct: 181 DQLTEVDLSDFVAGRPEAEALEVMNMFSSALEGSKLRYLNLSDNALGEKGIRAFASLINS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           Q  LEELYLMNDGISE+AARA+ EL+PSTDK+R+LQFHNNMTGDEGA AI+EIV+  P+L
Sbjct: 241 QHDLEELYLMNDGISEDAARAVRELLPSTDKIRVLQFHNNMTGDEGATAIAEIVRECPSL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTR+G EGGVALAEA+  C+HLKKLDLRDNMFGVE GIAL+K++S    LTEIY
Sbjct: 301 EDFRCSSTRIGSEGGVALAEALEHCSHLKKLDLRDNMFGVEGGIALAKTLSVLTHLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           +SYLNLEDEG EAL+ AL  SAPSLEVLE+AGNDIT K   ++AAC+A+KQ L+KL L+E
Sbjct: 361 MSYLNLEDEGTEALSEALLKSAPSLEVLELAGNDITVKSTGNLAACIASKQSLAKLNLSE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKD+G ILI+KA+ +GH QL EVD+STN IRRAGAR LAQ +V+K  FKLLNINGN+I
Sbjct: 421 NELKDEGTILIAKAV-EGHDQLVEVDLSTNMIRRAGARALAQTVVKKNTFKLLNINGNFI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDY-DEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEV D+FK+    L PLD+NDPEGED+ DED EE G+  +ELESKL  L IK+ E
Sbjct: 481 SEEGIDEVNDMFKDCLDKLVPLDDNDPEGEDFEDEDEEEEGEDGNELESKLGSLKIKQGE 534

BLAST of Carg25776 vs. TAIR 10
Match: AT3G63130.2 (RAN GTPase activating protein 1 )

HSP 1 Score: 722.2 bits (1863), Expect = 3.0e-208
Identity = 374/540 (69.26%), Postives = 452/540 (83.70%), Query Frame = 0

Query: 1   MDSAAQTFPPRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKK 60
           MD +A+T   RV+S+K+WPPS+STR ML+ER+ KN+TTPSIFSRKYGLLS EEAE+DAK+
Sbjct: 1   MDHSAKTTQNRVLSVKMWPPSKSTRLMLVERMTKNITTPSIFSRKYGLLSVEEAEQDAKR 60

Query: 61  VEDIAFATANQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA 120
           +ED+AFATAN+HF+ EPDGDG+SAV +YAKESS+LMLD++KRGP  +E+ E  +S+    
Sbjct: 61  IEDLAFATANKHFQNEPDGDGTSAVHVYAKESSKLMLDVIKRGP--QEESEVEVSKDG-- 120

Query: 121 KGTVFDISGGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIK 180
               FDISGG RAFI+ EEA++LL PL DP N +TKI FSNRSFG +AA+ A  +L SIK
Sbjct: 121 -DVFFDISGGSRAFIEEEEARDLLRPLADPRNSYTKIRFSNRSFGSEAAKFAASVLSSIK 180

Query: 181 DRLTEVDLSDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKS 240
           D+LTEVDLSDF+AGRPE EALEV+N+FSSALEG  LR L+LS+NA+GEKG+RAF SL+ S
Sbjct: 181 DQLTEVDLSDFVAGRPEAEALEVMNMFSSALEGSKLRYLNLSDNALGEKGIRAFASLINS 240

Query: 241 QKHLEELYLMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPAL 300
           Q  LEELYLMNDGISE+AARA+ EL+PSTDK+R+LQFHNNMTGDEGA AI+EIV+  P+L
Sbjct: 241 QHDLEELYLMNDGISEDAARAVRELLPSTDKIRVLQFHNNMTGDEGATAIAEIVRECPSL 300

Query: 301 EDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIY 360
           EDFRCSSTR+G EGGVALAEA+  C+HLKKLDLRDNMFGVE GIAL+K++S    LTEIY
Sbjct: 301 EDFRCSSTRIGSEGGVALAEALEHCSHLKKLDLRDNMFGVEGGIALAKTLSVLTHLTEIY 360

Query: 361 LSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAE 420
           +SYLNLEDEG EAL+ AL  SAPSLEVLE+AGNDIT K   ++AAC+A+KQ L+KL L+E
Sbjct: 361 MSYLNLEDEGTEALSEALLKSAPSLEVLELAGNDITVKSTGNLAACIASKQSLAKLNLSE 420

Query: 421 NELKDDGVILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYI 480
           NELKD+G ILI+KA+ +GH QL EVD+STN IRRAGAR LAQ +V+K  FKLLNINGN+I
Sbjct: 421 NELKDEGTILIAKAV-EGHDQLVEVDLSTNMIRRAGARALAQTVVKKNTFKLLNINGNFI 480

Query: 481 SEEGIDEVKDIFKNSPSMLGPLDENDPEGEDY-DEDAEENGDHDDELESKLKGLDIKRDE 540
           SEEGIDEV D+FK+    L PLD+NDPEGED+ DED EE G+  +ELESKL  L IK+ E
Sbjct: 481 SEEGIDEVNDMFKDCLDKLVPLDDNDPEGEDFEDEDEEEEGEDGNELESKLGSLKIKQGE 534

BLAST of Carg25776 vs. TAIR 10
Match: AT5G19320.1 (RAN GTPase activating protein 2 )

HSP 1 Score: 696.4 bits (1796), Expect = 1.8e-200
Identity = 352/537 (65.55%), Postives = 447/537 (83.24%), Query Frame = 0

Query: 10  PRVMSIKLWPPSQSTRFMLIERIIKNLTTPSIFSRKYGLLSKEEAEEDAKKVEDIAFATA 69
           P   SIKLWPPS  TR  LIERI  N ++ +IF+ KYG L+K++A E+AK++EDIAF+TA
Sbjct: 9   PHAFSIKLWPPSLPTRKALIERITNNFSSKTIFTEKYGSLTKDQATENAKRIEDIAFSTA 68

Query: 70  NQHFEKEPDGDGSSAVQIYAKESSRLMLDILKRGPVLKEDGEAIISERAAA-KGTVFDIS 129
           NQ FE+EPDGDG SAVQ+YAKE S+L+L++LK+GPV K     +ISE + + + T FDIS
Sbjct: 69  NQQFEREPDGDGGSAVQLYAKECSKLILEVLKKGPVAKVAARELISEDSVSPRETFFDIS 128

Query: 130 GGRRAFIDAEEAKELLEPLKDPGNLFTKICFSNRSFGLDAARVAEPILMSIKDRLTEVDL 189
            G+RAFI+AEEA+ELL+PLK+PGN +TKICFSNRSFGL AARVAEPIL S+KD+L EVDL
Sbjct: 129 KGKRAFIEAEEAEELLKPLKEPGNAYTKICFSNRSFGLGAARVAEPILASLKDQLKEVDL 188

Query: 190 SDFIAGRPEGEALEVVNIFSSALEGCDLRSLDLSNNAMGEKGVRAFGSLLKSQKHLEELY 249
           SDF+AGRPE EALEV+NIFS AL+G  L SL+LS+NA+GEKGVRAFG+LLKS   LEELY
Sbjct: 189 SDFVAGRPELEALEVMNIFSDALQGSILSSLNLSDNALGEKGVRAFGALLKSLSSLEELY 248

Query: 250 LMNDGISEEAARAICELIPSTDKLRILQFHNNMTGDEGAIAISEIVKGSPALEDFRCSST 309
           LMNDGIS+EAA+A+ ELIPST+ LR+L FHNNMTGDEGA+AI+E+VK SP LE+FRCSST
Sbjct: 249 LMNDGISKEAAQAVSELIPSTENLRVLHFHNNMTGDEGALAIAEVVKRSPLLENFRCSST 308

Query: 310 RVGCEGGVALAEAIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIYLSYLNLED 369
           RVG +GG+AL+EA+  CTH++KLDLRDNMFG EAG++LSK++S+F  +TE+YLSYLNLED
Sbjct: 309 RVGSKGGIALSEALEHCTHMEKLDLRDNMFGTEAGVSLSKTLSSFKHMTELYLSYLNLED 368

Query: 370 EGAEALANALKDSAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAENELKDDGV 429
           EGA A+ NALK+SA  +EVLEMAGNDIT + A++IAACVA KQ L+KL L+ENELKD+G 
Sbjct: 369 EGAIAIVNALKESASPIEVLEMAGNDITVEAASAIAACVAAKQDLNKLNLSENELKDEGC 428

Query: 430 ILISKALQDGHGQLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYISEEGIDEV 489
           + I+  +++GH +L  +D+STN IRRAGAR LA ++V+K  FKLLNI+GN ISEEGI+E+
Sbjct: 429 VQIANCIEEGHSKLQYIDMSTNYIRRAGARALAHVVVKKEAFKLLNIDGNIISEEGIEEL 488

Query: 490 KDIFKNSPSMLGPLDENDPEGEDYDEDAEENGDHDD------ELESKLKGLDIKRDE 540
           K+IFK SP +LG LDENDP+GE+ D+D E+  D ++      ELESKLK L++ +++
Sbjct: 489 KEIFKKSPELLGALDENDPDGEEDDDDEEDEEDEENEGNGNGELESKLKNLEVNQED 545

BLAST of Carg25776 vs. TAIR 10
Match: AT3G06000.1 (RNI-like superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 3.1e-43
Identity = 101/203 (49.75%), Postives = 140/203 (68.97%), Query Frame = 0

Query: 321 AIGTCTHLKKLDLRDNMFGVEAGIALSKSISAFPGLTEIYLSYLNLEDEGAEALANALKD 380
           A  TCTH+K             G+++SK  S+F  LT I LSY NLE+ GA AL NALK+
Sbjct: 16  AFETCTHIK------------VGVSMSKPFSSFSFLTVINLSYTNLENGGAIALVNALKN 75

Query: 381 SAPSLEVLEMAGNDITAKGAASIAACVATKQFLSKLYLAENELKDDGVILISKALQDGHG 440
           SAPSL+V+EMAGN+IT + A +IA C+A K+ L KL L+EN+LKD+G + I K+++D   
Sbjct: 76  SAPSLQVIEMAGNNITYEAATAIAVCLAAKRHLKKLNLSENDLKDEGCVEIVKSMED--W 135

Query: 441 QLSEVDVSTNSIRRAGARFLAQILVQKPGFKLLNINGNYISEEGIDEVKDIFKNSPSMLG 500
           +L  VD+S N +RR GA  LA+++V+K  FK+LNI+GN IS +GI+E+K IF N P +LG
Sbjct: 136 ELEYVDMSYNDLRREGALRLARVVVKKGSFKMLNIDGNMISLKGIEEIKVIFTNCPKLLG 195

Query: 501 PLDENDPEGEDYDEDAEENGDHD 524
           PLD+N    +D D+D  EN + +
Sbjct: 196 PLDKNVYNVDD-DDDLRENDEDE 203

BLAST of Carg25776 vs. TAIR 10
Match: AT1G10510.1 (RNI-like superfamily protein )

HSP 1 Score: 119.4 bits (298), Expect = 8.9e-27
Identity = 76/278 (27.34%), Postives = 132/278 (47.48%), Query Frame = 0

Query: 216 LRSLDLSNNAMGEKGVRAFGSLLKSQKHLEELYLMNDGISEEAARAICELIPSTDKLRIL 275
           +  +  S N +   GV+AF  +L+S   L+ L L  + I +E A+ +C  +     + IL
Sbjct: 200 VEEVSFSANGITAAGVKAFDGVLQSNIMLKILNLSGNPIGDEGAKTLCATLMENSSIEIL 259

Query: 276 QFHNNMTGDEGAIAISEIVKGSPALEDFRCSSTRVGCEGGVALAEAIGTCTHLKKLDLRD 335
           Q ++   GDEGA  I+E++K +  L     ++  +   G  +LA A+     ++ L L  
Sbjct: 260 QLNSTDIGDEGAKEIAELLKRNSTLRIIELNNNMIDYSGFTSLAGALLENNTIRNLHLNG 319

Query: 336 NMFGVEAGIALSKSISAFPGLTEIYLSYLNLEDEGAEALANALKDSAPSLEVLEMAGNDI 395
           N  G     AL+K +     L E++L   ++ DEG  AL   L      + +L++  N I
Sbjct: 320 NYGGALGANALAKGLEGNKSLRELHLHGNSIGDEGTRALMAGLSSHKGKVALLDLGNNSI 379

Query: 396 TAKGAASIAACVATKQFLSKLYLAENELKDDGVILISKALQDGHGQLSEVDVSTNSIRRA 455
           +AKGA  +A  +   + L  L L  N++ D+G   I+ +L+     ++ +D+  N+I   
Sbjct: 380 SAKGAFYVAEYIKRSKSLVWLNLYMNDIGDEGAEKIADSLKQNR-SIATIDLGGNNIHAE 439

Query: 456 GARFLAQILVQKPGFKLLNINGNYISEEGIDEVKDIFK 494
           G   +AQ L        L +  N I  +G   + +I K
Sbjct: 440 GVNAIAQALKDNAIITTLEVGYNPIGPDGAKALSEILK 476

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025494.17.0e-300100.00RAN GTPase-activating protein 1, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
KAG6593086.12.9e-29899.44RAN GTPase-activating protein 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022960216.13.8e-29899.63RAN GTPase-activating protein 1-like [Cucurbita moschata][more]
XP_023004254.15.0e-29899.63RAN GTPase-activating protein 1-like [Cucurbita maxima] >XP_023514749.1 RAN GTPa... [more]
XP_038898694.13.0e-28293.14RAN GTPase-activating protein 1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9LE824.2e-20769.26RAN GTPase-activating protein 1 OS=Arabidopsis thaliana OX=3702 GN=RANGAP1 PE=1 ... [more]
Q9M6512.5e-19965.55RAN GTPase-activating protein 2 OS=Arabidopsis thaliana OX=3702 GN=RANGAP2 PE=1 ... [more]
Q5DU562.8e-2525.80Protein NLRC3 OS=Mus musculus OX=10090 GN=Nlrc3 PE=1 SV=2[more]
Q7RTR24.8e-2530.52NLR family CARD domain-containing protein 3 OS=Homo sapiens OX=9606 GN=NLRC3 PE=... [more]
P460619.0e-2427.20Ran GTPase-activating protein 1 OS=Mus musculus OX=10090 GN=Rangap1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1H7101.9e-29899.63RAN GTPase-activating protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114610... [more]
A0A6J1KVR82.4e-29899.63RAN GTPase-activating protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497640... [more]
A0A5A7UUV63.5e-28192.95RAN GTPase-activating protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BRW21.3e-28092.76RAN GTPase-activating protein 1 OS=Cucumis melo OX=3656 GN=LOC103492625 PE=4 SV=... [more]
A0A0A0K7649.9e-27691.28WPP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378480 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT3G63130.13.0e-20869.26RAN GTPase activating protein 1 [more]
AT3G63130.23.0e-20869.26RAN GTPase activating protein 1 [more]
AT5G19320.11.8e-20065.55RAN GTPase activating protein 2 [more]
AT3G06000.13.1e-4349.75RNI-like superfamily protein [more]
AT1G10510.18.9e-2727.34RNI-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00368LRR_RI_2coord: 410..437
e-value: 0.11
score: 21.5
coord: 269..296
e-value: 34.0
score: 6.4
coord: 325..352
e-value: 0.64
score: 18.5
coord: 382..409
e-value: 0.21
score: 20.7
coord: 467..494
e-value: 100.0
score: 3.0
coord: 439..466
e-value: 64.0
score: 4.4
coord: 353..380
e-value: 0.54
score: 19.0
coord: 241..268
e-value: 0.46
score: 19.5
coord: 297..324
e-value: 10.0
score: 10.1
coord: 213..240
e-value: 2.4E-4
score: 30.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 523..539
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 504..522
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 495..539
NoneNo IPR availablePANTHERPTHR46761RAN GTPASE-ACTIVATING PROTEIN 1coord: 1..539
NoneNo IPR availablePANTHERPTHR46761:SF5RAN GTPASE-ACTIVATING PROTEIN 2coord: 1..539
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 125..517
IPR001611Leucine-rich repeatPFAMPF13516LRR_6coord: 356..375
e-value: 0.13
score: 12.4
coord: 324..347
e-value: 0.031
score: 14.3
coord: 383..404
e-value: 0.018
score: 15.1
coord: 215..234
e-value: 0.16
score: 12.1
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 409..503
e-value: 4.4E-12
score: 47.8
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 130..408
e-value: 6.3E-60
score: 204.6
IPR038214WPP domain superfamilyGENE3D1.10.246.200WPP domaincoord: 14..111
e-value: 9.8E-35
score: 120.9
IPR025265WPP domainPFAMPF13943WPPcoord: 14..108
e-value: 9.8E-35
score: 118.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25776-RACarg25776-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding