Carg05249-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05249-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein of unknown function, DUF547
LocationCarg_Chr07: 3746257 .. 3750632 (-)
Sequence length1993
RNA-Seq ExpressionCarg05249-RA
SyntenyCarg05249-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTAACTAATGGTTTACTGATAGTGTTGATACTCAGAGATGCTGTTTCAGGAGACTGACGGCGGTGCAAAAATGCTACAAAACAGACAGATTCCATTACACAAGCATTCCAAGAGGTTAGCTTCTGATGCTTTGTTTTGTTATTTAGCAATGATTGTTCATTCTGTTCTGAACACTTGATGTGAACTTGGACTGGAAAGCTTCTTAGTTGGTTGAATAGTGAAGGAGTTTGACAATTGGTTCCTGTCGGCTTACCAAACTTTGAAATCAGATGTCTAAAGACATAGATCTTGGTTTTCTGCTCCAAGAGCTTAATAACTTCTTCTTTCTTATTGTCTTTCAAGCATCATTTTTCTGAGATTAGTAATGAGGTTGGGTTTGGAACCTTTTGACAATTAGCTCCTCCTTTTCTTCTGCTTCTTCAGATCTGTAATATTTGTACTGTTTCAGTCTCTTTACTTTTGCTATCCTTGTGAGAGAATAGAGAAAATGGGAGTGTTAAATAACTGTGTCACTTTGAGTTTTGGGGAATTATGAGCTACCTAGAGAATGTAGAAGGAAAGTTAATGGTCTGATCCCACATCGGTTGGGGAGGAGAACGAAGCATTCTGTATAAAGGTGTGGAAACCTCTCCCAAAGCATTTTTTTATAAGGGTGTGAAAAACCTCTCCCTAGCAGATACATTTTAAATCTTGAGGGAAAGCTCGAAAGGGAAAGTCCGAAGAGGACAATAACGGCTAGCGGTGGACTTGGACACTGGATGATGTGTCAGTGAGGAGGTGAGGAGGCTAAACCCTGAAGGGGGTAGACACCAGGCAGTGTGCCAACGAGGACGCTGAGTCTCGAAGGGGAGTGGATTGTGAGATCCCACATCGGTTGGAGTGGGAAACAAAGCATTCTTTGTAAGGGTGTGGAAACCTCTCCCTAGCATACGCGTTTTAAAAACCTTGAGGGGAAGCTTGAAAGGGAAAGCTCAAAGAGGATAATATCTGCTAACGGTGGGCTTGGGTTGTTACATCATTATAGATAGATTTCGGGATAGTTCGGTTTTTGTAGCCCTTGCTCGGTGTAAATTATCGAGTCTTGCCTCGGTCGAGGATCTTATATAAAATGTTAACAAATGGAACTAACAAGCCAAGGTATTATCTTCTCCATTTCTGCATTTTAATTCTTCTTTGCTCTGTTTACAATGCAGCTTTCTAGACATGGTCAAGGATACTAACAAGGAAGAACACTTGGATAACTCCTTTGAAGCTTCAAATCGTGTGAAGCTGGTAAGTTAACTTACATTTATTATTTGGACCAAAAGACAGCTTATGGAGACAATGAATCTAGCATATTTTAAGGTTGTTTTTTTGGGTTTGATCTGATCCTAGGACACGAGTTCGTTCAAGGAATCCATGAACACAGAGAAGAAAAGCTTCCCAAAAACAAACATACACTCTTCTCTTAAGCAAGAGGTACAAAGAATCATTGACCTTCTAAGTCTCAACAAGGCTAAGTCCTTAAATGATAACCATTCTTCTATTAAACTATGCAATGCAGATTCTTCAGCTTGAGAAGAGACTGCAAGACCAGTTCAAGGTCCGCACATCACTAGAAAAAGCATTAGGATATAAGCCATCTTGTCAGGACAACACGAGCAGCGACACTGAAGTAGCCAAGGTAACTCGACGTTTTCGGTCTGTTATAATCTACATTATAGCTACCTATTAAAAGCTGCTTTGATGACTACAGCCAGCCACTGAATTGATCAAAGAGATCACTGTATTGGAGCTAGAAGTTTCACATTTAGAACAGTATCTTCTGTCGTTGTATCGAAAAGCATTTGATGGACAAATAGCTTCTACATCTCCTATGAACTCCCCAAAATCCAAGTATATGATGAATTGCATACCTGACATAACGTCGAAAAAGGAAGATAAAGCCGTTCAGTCTGGCTACGATTCTTTTGGAAATCCCGTGAAGGAATACAGCGGAATTCGTGAAAACAAGCTGTTGGACTCCCGCGTTCGTCGTTGTCAGTCGTCGTTGTCACATTATTCAGTTAGCTCAAAGAGAACTTATCTCCCAGATGATTCTTTGTGTCAAGCTGTACGTCCTTGTCTATCCCAACCAATGTCCATGATAGAGGTAGCTGTCTTCTGTTTCATTAAACTTGTTCTTATGCAAACTTTACTAGACATAATAGTGCAATATCAAAGAACTTGCTCTATGGGCCATAATAGATATCTATTCTTCTTTGTTTATGGTTCCTTTTTCTGCAGTTTGCTCGGAATGCTTCATCCAATTTAACCAGTCTAGCAGACTACCTTGGTACTCAAATACTAGACCATATTCCCGAGAGTGCGAATCGACTTTCGGAAGATATGATCAAGTGCATATCAGCCATATATTGCAAACTTTCAGATCCTCCTTTATCACATCATGGTTTATCCTCCCCCGTCTCGTCGTCTTCGCCAATCAGTGCGTTCTCTCCACAAGATCAATTTGATATGTTGAGTCCTGGATTCAGAAACAATTCATCTTTTGATATGATGTTGGATAATCCTTTTCACGTCGAAGGTCTTAAAGAGTTTAGCGGCCCGTACAGCACAATGGTTGAAGTGCCATCGATTTATAGGGATAGTCAGAAGTTGATCGAAGTCGAGCACTTGTTACAAGATTTCAGGTAAGCAACGTGTGGATGTTCATAGTCATATTGCTAGAAGATTTTGTTTGATGGAACGTGATTATGTAACAGTCCAAGTCTACCGCTTGCAGATATTGTTCTTTTTAGGCTTTCCCTCAAGATTTTAAAACACCTCTACTATGAGCTGTTACAAATGATATCAGAGCCGGACACCACATCGGTTGGAGAGGGGAACGAAACATTCCTTATAAGGGTGTGGAAACCTTTCCCTAGGAGACGCGTTTTAAAACCTTGAGAGGAAGCCCTGGAAGGGAAAGCCCAAAGAAGACAGTATCTGCTAGCTATGGGTTTGGGCGGTTACCGATTGACTGATTGCTTCTTATTCGTTTTATATTTACCGGTTATACTTATATGTGGTCGTTAGGTCGCTTATATCCAAGTTAGAAGAAGTCGATCCGAGGAAGTTGAATCATGAGGAAAAGCTAGCATTCTGGATAAACGTACACAATTCACTGATGATGCACGTAAAGAGTTTTCATAACTTGAACTTCCTGTCCTTTTAGTTCTTCAAAGACAATCACACTATAATATCTGTTTTGTTTTCTGAAAGGCATACTTGGCTTATGGTATACCACACAACAATATGAAGAAGGTTTTCGTTCTCTTGAAGGTAAACACGAGTCCCGATGATCCGTTATGATTTGTAAATGCTATATGATTATCGTGTTCTTACTTTCGTCCTCCATTCAGGCTGCATATAACATAGGAGGTCAAACCATCAGCGTCGACACCATACAAAGTTCAATACTTGGATGCCGAGTGCCTCGGCCTGGACAGGTACACACTATAGACAAAAGTATAAGCAATGTCACTCACAAAGTGTAAATGGCTTCTTAGTTTGTACGATCATGTGAGATCCCACATCGGTTAAGGGTGTGGAAACTCTCCCTAGTAGACGCGTTTTAAAACTGTGAGGCTAACGGTGATATGTAACGGGCCAAAACAGACAATATCTACTAGCGGGAAGTCAACATTAAGATATAACTTTCTTCTGTTTGCAGTGGCTCTCTCTTCTTATTCCCTCGAAGTCAAAGTTCAAAAACGGAGATAAACGACAAGCGTACAAAATCGACCGGTCGGAACCCCTTTTACATTTTGCACTTTGCACAGGCTGCCATTCTGATCCTGCGGTACTTGAAACAACATTAGTGAAACTACATGTGTACTTGTCCTAAAGGTTTGACACTTTGTGCTATGTTCTAATATACTAACTTTCCCATCTTCCAGGTTCGTGTATACACTCCCAAAAGAGTCCTGCAGGAGCTGGAAACAGCAAAAGAAGAATACATACGAGCCACATTCGGCATACGCAAGGACAAAAAAGTCATTTTACCAAAGATGATCGAGTCATTTGCAAAAGAATCAAGATTGTGCACGGCTGGTACGATGGAAATGATTCAGAAGTCATTGCCTGAATCACTTAGGAAGAGTGTTACGAAATGTCAAAACGGGAAATCTCGCAAGAACATCGAATGGATACCGCACAACTTCGGTTTTCGATATCTGATATGTAGAGAAATGAAGTGATATCTCTGATGAACATAGCTGCTGCTTACATGTTGTTGATGATGAACTGAAATCCTTGAAGAGGGTTACATATTTTCTTCCTTCTGTACATAGCATTATACCATCTAGGATTTTAAATTTTTTGTTGTAAATAAGTTTGACTTTTGAAGATCAGTTC

mRNA sequence

CCCTAACTAATGGTTTACTGATAGTGTTGATACTCAGAGATGCTGTTTCAGGAGACTGACGGCGGTGCAAAAATGCTACAAAACAGACAGATTCCATTACACAAGCATTCCAAGAGCTTTCTAGACATGGTCAAGGATACTAACAAGGAAGAACACTTGGATAACTCCTTTGAAGCTTCAAATCGTGTGAAGCTGGACACGAGTTCGTTCAAGGAATCCATGAACACAGAGAAGAAAAGCTTCCCAAAAACAAACATACACTCTTCTCTTAAGCAAGAGATTCTTCAGCTTGAGAAGAGACTGCAAGACCAGTTCAAGGTCCGCACATCACTAGAAAAAGCATTAGGATATAAGCCATCTTGTCAGGACAACACGAGCAGCGACACTGAAGTAGCCAAGCCAGCCACTGAATTGATCAAAGAGATCACTGTATTGGAGCTAGAAGTTTCACATTTAGAACAGTATCTTCTGTCGTTGTATCGAAAAGCATTTGATGGACAAATAGCTTCTACATCTCCTATGAACTCCCCAAAATCCAAGTATATGATGAATTGCATACCTGACATAACGTCGAAAAAGGAAGATAAAGCCGTTCAGTCTGGCTACGATTCTTTTGGAAATCCCGTGAAGGAATACAGCGGAATTCGTGAAAACAAGCTGTTGGACTCCCGCGTTCGTCGTTGTCAGTCGTCGTTGTCACATTATTCAGTTAGCTCAAAGAGAACTTATCTCCCAGATGATTCTTTGTGTCAAGCTTTTGCTCGGAATGCTTCATCCAATTTAACCAGTCTAGCAGACTACCTTGGTACTCAAATACTAGACCATATTCCCGAGAGTGCGAATCGACTTTCGGAAGATATGATCAAGTGCATATCAGCCATATATTGCAAACTTTCAGATCCTCCTTTATCACATCATGGTTTATCCTCCCCCGTCTCGTCGTCTTCGCCAATCAGTGCGTTCTCTCCACAAGATCAATTTGATATGTTGAGTCCTGGATTCAGAAACAATTCATCTTTTGATATGATGTTGGATAATCCTTTTCACGTCGAAGGTCTTAAAGAGTTTAGCGGCCCGTACAGCACAATGGTTGAAGTGCCATCGATTTATAGGGATAGTCAGAAGTTGATCGAAGTCGAGCACTTGTTACAAGATTTCAGGTCGCTTATATCCAAGTTAGAAGAAGTCGATCCGAGGAAGTTGAATCATGAGGAAAAGCTAGCATTCTGGATAAACGTACACAATTCACTGATGATGCACGCATACTTGGCTTATGGTATACCACACAACAATATGAAGAAGGTTTTCGTTCTCTTGAAGGCTGCATATAACATAGGAGGTCAAACCATCAGCGTCGACACCATACAAAGTTCAATACTTGGATGCCGAGTGCCTCGGCCTGGACAGTGGCTCTCTCTTCTTATTCCCTCGAAGTCAAAGTTCAAAAACGGAGATAAACGACAAGCGTACAAAATCGACCGGTCGGAACCCCTTTTACATTTTGCACTTTGCACAGGCTGCCATTCTGATCCTGCGGTTCGTGTATACACTCCCAAAAGAGTCCTGCAGGAGCTGGAAACAGCAAAAGAAGAATACATACGAGCCACATTCGGCATACGCAAGGACAAAAAAGTCATTTTACCAAAGATGATCGAGTCATTTGCAAAAGAATCAAGATTGTGCACGGCTGGTACGATGGAAATGATTCAGAAGTCATTGCCTGAATCACTTAGGAAGAGTGTTACGAAATGTCAAAACGGGAAATCTCGCAAGAACATCGAATGGATACCGCACAACTTCGGTTTTCGATATCTGATATGTAGAGAAATGAAGTGATATCTCTGATGAACATAGCTGCTGCTTACATGTTGTTGATGATGAACTGAAATCCTTGAAGAGGGTTACATATTTTCTTCCTTCTGTACATAGCATTATACCATCTAGGATTTTAAATTTTTTGTTGTAAATAAGTTTGACTTTTGAAGATCAGTTC

Coding sequence (CDS)

ATGCTGTTTCAGGAGACTGACGGCGGTGCAAAAATGCTACAAAACAGACAGATTCCATTACACAAGCATTCCAAGAGCTTTCTAGACATGGTCAAGGATACTAACAAGGAAGAACACTTGGATAACTCCTTTGAAGCTTCAAATCGTGTGAAGCTGGACACGAGTTCGTTCAAGGAATCCATGAACACAGAGAAGAAAAGCTTCCCAAAAACAAACATACACTCTTCTCTTAAGCAAGAGATTCTTCAGCTTGAGAAGAGACTGCAAGACCAGTTCAAGGTCCGCACATCACTAGAAAAAGCATTAGGATATAAGCCATCTTGTCAGGACAACACGAGCAGCGACACTGAAGTAGCCAAGCCAGCCACTGAATTGATCAAAGAGATCACTGTATTGGAGCTAGAAGTTTCACATTTAGAACAGTATCTTCTGTCGTTGTATCGAAAAGCATTTGATGGACAAATAGCTTCTACATCTCCTATGAACTCCCCAAAATCCAAGTATATGATGAATTGCATACCTGACATAACGTCGAAAAAGGAAGATAAAGCCGTTCAGTCTGGCTACGATTCTTTTGGAAATCCCGTGAAGGAATACAGCGGAATTCGTGAAAACAAGCTGTTGGACTCCCGCGTTCGTCGTTGTCAGTCGTCGTTGTCACATTATTCAGTTAGCTCAAAGAGAACTTATCTCCCAGATGATTCTTTGTGTCAAGCTTTTGCTCGGAATGCTTCATCCAATTTAACCAGTCTAGCAGACTACCTTGGTACTCAAATACTAGACCATATTCCCGAGAGTGCGAATCGACTTTCGGAAGATATGATCAAGTGCATATCAGCCATATATTGCAAACTTTCAGATCCTCCTTTATCACATCATGGTTTATCCTCCCCCGTCTCGTCGTCTTCGCCAATCAGTGCGTTCTCTCCACAAGATCAATTTGATATGTTGAGTCCTGGATTCAGAAACAATTCATCTTTTGATATGATGTTGGATAATCCTTTTCACGTCGAAGGTCTTAAAGAGTTTAGCGGCCCGTACAGCACAATGGTTGAAGTGCCATCGATTTATAGGGATAGTCAGAAGTTGATCGAAGTCGAGCACTTGTTACAAGATTTCAGGTCGCTTATATCCAAGTTAGAAGAAGTCGATCCGAGGAAGTTGAATCATGAGGAAAAGCTAGCATTCTGGATAAACGTACACAATTCACTGATGATGCACGCATACTTGGCTTATGGTATACCACACAACAATATGAAGAAGGTTTTCGTTCTCTTGAAGGCTGCATATAACATAGGAGGTCAAACCATCAGCGTCGACACCATACAAAGTTCAATACTTGGATGCCGAGTGCCTCGGCCTGGACAGTGGCTCTCTCTTCTTATTCCCTCGAAGTCAAAGTTCAAAAACGGAGATAAACGACAAGCGTACAAAATCGACCGGTCGGAACCCCTTTTACATTTTGCACTTTGCACAGGCTGCCATTCTGATCCTGCGGTTCGTGTATACACTCCCAAAAGAGTCCTGCAGGAGCTGGAAACAGCAAAAGAAGAATACATACGAGCCACATTCGGCATACGCAAGGACAAAAAAGTCATTTTACCAAAGATGATCGAGTCATTTGCAAAAGAATCAAGATTGTGCACGGCTGGTACGATGGAAATGATTCAGAAGTCATTGCCTGAATCACTTAGGAAGAGTGTTACGAAATGTCAAAACGGGAAATCTCGCAAGAACATCGAATGGATACCGCACAACTTCGGTTTTCGATATCTGATATGTAGAGAAATGAAGTGA

Protein sequence

MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAKPATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKKEDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQAFARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNFGFRYLICREMK
Homology
BLAST of Carg05249-RA vs. NCBI nr
Match: KAG7027088.1 (hypothetical protein SDJN02_11098 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1184.9 bits (3064), Expect = 0.0e+00
Identity = 598/598 (100.00%), Postives = 598/598 (100.00%), Query Frame = 0

Query: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60
           MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES
Sbjct: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60

Query: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK 120
           MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK
Sbjct: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK 120

Query: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK 180
           PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK
Sbjct: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK 180

Query: 181 EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQAF 240
           EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQAF
Sbjct: 181 EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQAF 240

Query: 241 ARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLSHHGLSSPVS 300
           ARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLSHHGLSSPVS
Sbjct: 241 ARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLSHHGLSSPVS 300

Query: 301 SSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMVEVPSIYRDS 360
           SSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMVEVPSIYRDS
Sbjct: 301 SSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMVEVPSIYRDS 360

Query: 361 QKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLAYGIPHNNMK 420
           QKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLAYGIPHNNMK
Sbjct: 361 QKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLAYGIPHNNMK 420

Query: 421 KVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNGDKRQAYKID 480
           KVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNGDKRQAYKID
Sbjct: 421 KVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNGDKRQAYKID 480

Query: 481 RSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKKVILPKMIES 540
           RSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKKVILPKMIES
Sbjct: 481 RSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKKVILPKMIES 540

Query: 541 FAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNFGFRYLICREMK 599
           FAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNFGFRYLICREMK
Sbjct: 541 FAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNFGFRYLICREMK 598

BLAST of Carg05249-RA vs. NCBI nr
Match: XP_022962712.1 (uncharacterized protein LOC111463123 isoform X1 [Cucurbita moschata] >XP_022962713.1 uncharacterized protein LOC111463123 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 597/611 (97.71%), Postives = 598/611 (97.87%), Query Frame = 0

Query: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60
           MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES
Sbjct: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60

Query: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK 120
           MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK
Sbjct: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK 120

Query: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK 180
           PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK
Sbjct: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK 180

Query: 181 EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA- 240
           EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA 
Sbjct: 181 EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQAV 240

Query: 241 ------------FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 300
                       FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD
Sbjct: 241 RPCLSQPMSMIEFARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 300

Query: 301 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY 360
           PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY
Sbjct: 301 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY 360

Query: 361 STMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH 420
           STMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH
Sbjct: 361 STMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH 420

Query: 421 AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSK 480
           AYLAYGIPHNNMKKVF+LLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSK
Sbjct: 421 AYLAYGIPHNNMKKVFILLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSK 480

Query: 481 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 540
           FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR
Sbjct: 481 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 540

Query: 541 KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF 599
           KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF
Sbjct: 541 KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF 600

BLAST of Carg05249-RA vs. NCBI nr
Match: XP_022962714.1 (uncharacterized protein LOC111463123 isoform X2 [Cucurbita moschata] >XP_022962716.1 uncharacterized protein LOC111463123 isoform X3 [Cucurbita moschata])

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 593/607 (97.69%), Postives = 594/607 (97.86%), Query Frame = 0

Query: 5   ETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTE 64
           ETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTE
Sbjct: 2   ETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTE 61

Query: 65  KKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAKPATE 124
           KKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAKPATE
Sbjct: 62  KKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAKPATE 121

Query: 125 LIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKKEDKA 184
           LIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKKEDKA
Sbjct: 122 LIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKKEDKA 181

Query: 185 VQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA----- 244
           VQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA     
Sbjct: 182 VQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQAVRPCL 241

Query: 245 --------FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLS 304
                   FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLS
Sbjct: 242 SQPMSMIEFARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLS 301

Query: 305 HHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMV 364
           HHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMV
Sbjct: 302 HHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMV 361

Query: 365 EVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLA 424
           EVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLA
Sbjct: 362 EVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLA 421

Query: 425 YGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNG 484
           YGIPHNNMKKVF+LLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNG
Sbjct: 422 YGIPHNNMKKVFILLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNG 481

Query: 485 DKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKK 544
           DKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKK
Sbjct: 482 DKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKK 541

Query: 545 VILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNFGFRY 599
           VILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNFGFRY
Sbjct: 542 VILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNFGFRY 601

BLAST of Carg05249-RA vs. NCBI nr
Match: KAG6595073.1 (hypothetical protein SDJN03_11626, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 591/611 (96.73%), Postives = 592/611 (96.89%), Query Frame = 0

Query: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60
           MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES
Sbjct: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60

Query: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK 120
           MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK
Sbjct: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK 120

Query: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK 180
           PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK
Sbjct: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK 180

Query: 181 EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA- 240
           EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA 
Sbjct: 181 EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQAV 240

Query: 241 ------------FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 300
                       FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD
Sbjct: 241 RPCLSQPMSMIEFARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 300

Query: 301 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY 360
           PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY
Sbjct: 301 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY 360

Query: 361 STMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH 420
           STMVEVPSIYRDSQKLIEVEHL     SLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH
Sbjct: 361 STMVEVPSIYRDSQKLIEVEHL-----SLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH 420

Query: 421 AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSK 480
           AYLAYGIPHNNMKKVFVLLKAAYNIGGQTIS+DTIQSSILGCRVPRPGQWLSLLIPSKSK
Sbjct: 421 AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISIDTIQSSILGCRVPRPGQWLSLLIPSKSK 480

Query: 481 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 540
           FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR
Sbjct: 481 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 540

Query: 541 KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF 599
           KDKKVILPKMIESFAKESRLCTAG MEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF
Sbjct: 541 KDKKVILPKMIESFAKESRLCTAGMMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF 600

BLAST of Carg05249-RA vs. NCBI nr
Match: XP_023003592.1 (uncharacterized protein LOC111497149 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 583/611 (95.42%), Postives = 590/611 (96.56%), Query Frame = 0

Query: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60
           MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES
Sbjct: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60

Query: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK 120
           MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQD TSSD E+AK
Sbjct: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDGTSSDIELAK 120

Query: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK 180
           PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQI+STSP+NSP+SKYMMNCIPDITSKK
Sbjct: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQISSTSPINSPRSKYMMNCIPDITSKK 180

Query: 181 EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA- 240
           EDKAVQSGYDSFGNP+K YSGIRENKLLDSRVRRCQSSLS+YSVSSKRTYLPDDSLCQA 
Sbjct: 181 EDKAVQSGYDSFGNPMKGYSGIRENKLLDSRVRRCQSSLSYYSVSSKRTYLPDDSLCQAV 240

Query: 241 ------------FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 300
                       FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD
Sbjct: 241 RPCLSQPMSMIEFARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 300

Query: 301 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY 360
           PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDM LDNPFHVEGLKEFSGPY
Sbjct: 301 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMKLDNPFHVEGLKEFSGPY 360

Query: 361 STMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH 420
           STMVEVPSIYRD+QKLIEVEHLLQDFRSLISKLEEVDP KLNHEEKLAFWINVHNSLMMH
Sbjct: 361 STMVEVPSIYRDNQKLIEVEHLLQDFRSLISKLEEVDPMKLNHEEKLAFWINVHNSLMMH 420

Query: 421 AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSK 480
           AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIP KSK
Sbjct: 421 AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPLKSK 480

Query: 481 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 540
           FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR
Sbjct: 481 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 540

Query: 541 KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF 599
           KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSV KCQNGKSRKNIEWIPHNF
Sbjct: 541 KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVAKCQNGKSRKNIEWIPHNF 600

BLAST of Carg05249-RA vs. ExPASy TrEMBL
Match: A0A6J1HFK6 (uncharacterized protein LOC111463123 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463123 PE=4 SV=1)

HSP 1 Score: 1175.2 bits (3039), Expect = 0.0e+00
Identity = 597/611 (97.71%), Postives = 598/611 (97.87%), Query Frame = 0

Query: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60
           MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES
Sbjct: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60

Query: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK 120
           MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK
Sbjct: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK 120

Query: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK 180
           PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK
Sbjct: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK 180

Query: 181 EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA- 240
           EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA 
Sbjct: 181 EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQAV 240

Query: 241 ------------FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 300
                       FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD
Sbjct: 241 RPCLSQPMSMIEFARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 300

Query: 301 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY 360
           PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY
Sbjct: 301 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY 360

Query: 361 STMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH 420
           STMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH
Sbjct: 361 STMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH 420

Query: 421 AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSK 480
           AYLAYGIPHNNMKKVF+LLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSK
Sbjct: 421 AYLAYGIPHNNMKKVFILLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSK 480

Query: 481 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 540
           FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR
Sbjct: 481 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 540

Query: 541 KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF 599
           KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF
Sbjct: 541 KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF 600

BLAST of Carg05249-RA vs. ExPASy TrEMBL
Match: A0A6J1HHW5 (uncharacterized protein LOC111463123 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111463123 PE=4 SV=1)

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 593/607 (97.69%), Postives = 594/607 (97.86%), Query Frame = 0

Query: 5   ETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTE 64
           ETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTE
Sbjct: 2   ETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTE 61

Query: 65  KKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAKPATE 124
           KKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAKPATE
Sbjct: 62  KKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAKPATE 121

Query: 125 LIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKKEDKA 184
           LIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKKEDKA
Sbjct: 122 LIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKKEDKA 181

Query: 185 VQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA----- 244
           VQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA     
Sbjct: 182 VQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQAVRPCL 241

Query: 245 --------FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLS 304
                   FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLS
Sbjct: 242 SQPMSMIEFARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLS 301

Query: 305 HHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMV 364
           HHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMV
Sbjct: 302 HHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMV 361

Query: 365 EVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLA 424
           EVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLA
Sbjct: 362 EVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLA 421

Query: 425 YGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNG 484
           YGIPHNNMKKVF+LLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNG
Sbjct: 422 YGIPHNNMKKVFILLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNG 481

Query: 485 DKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKK 544
           DKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKK
Sbjct: 482 DKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKK 541

Query: 545 VILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNFGFRY 599
           VILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNFGFRY
Sbjct: 542 VILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNFGFRY 601

BLAST of Carg05249-RA vs. ExPASy TrEMBL
Match: A0A6J1KN07 (uncharacterized protein LOC111497149 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497149 PE=4 SV=1)

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 583/611 (95.42%), Postives = 590/611 (96.56%), Query Frame = 0

Query: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60
           MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES
Sbjct: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60

Query: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK 120
           MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQD TSSD E+AK
Sbjct: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDGTSSDIELAK 120

Query: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK 180
           PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQI+STSP+NSP+SKYMMNCIPDITSKK
Sbjct: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQISSTSPINSPRSKYMMNCIPDITSKK 180

Query: 181 EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA- 240
           EDKAVQSGYDSFGNP+K YSGIRENKLLDSRVRRCQSSLS+YSVSSKRTYLPDDSLCQA 
Sbjct: 181 EDKAVQSGYDSFGNPMKGYSGIRENKLLDSRVRRCQSSLSYYSVSSKRTYLPDDSLCQAV 240

Query: 241 ------------FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 300
                       FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD
Sbjct: 241 RPCLSQPMSMIEFARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 300

Query: 301 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY 360
           PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDM LDNPFHVEGLKEFSGPY
Sbjct: 301 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMKLDNPFHVEGLKEFSGPY 360

Query: 361 STMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH 420
           STMVEVPSIYRD+QKLIEVEHLLQDFRSLISKLEEVDP KLNHEEKLAFWINVHNSLMMH
Sbjct: 361 STMVEVPSIYRDNQKLIEVEHLLQDFRSLISKLEEVDPMKLNHEEKLAFWINVHNSLMMH 420

Query: 421 AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSK 480
           AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIP KSK
Sbjct: 421 AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPLKSK 480

Query: 481 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 540
           FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR
Sbjct: 481 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 540

Query: 541 KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF 599
           KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSV KCQNGKSRKNIEWIPHNF
Sbjct: 541 KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVAKCQNGKSRKNIEWIPHNF 600

BLAST of Carg05249-RA vs. ExPASy TrEMBL
Match: A0A6J1KPN7 (uncharacterized protein LOC111497149 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497149 PE=4 SV=1)

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 583/611 (95.42%), Postives = 590/611 (96.56%), Query Frame = 0

Query: 1   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 60
           MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES
Sbjct: 2   MLFQETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKES 61

Query: 61  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAK 120
           MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQD TSSD E+AK
Sbjct: 62  MNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDGTSSDIELAK 121

Query: 121 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKK 180
           PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQI+STSP+NSP+SKYMMNCIPDITSKK
Sbjct: 122 PATELIKEITVLELEVSHLEQYLLSLYRKAFDGQISSTSPINSPRSKYMMNCIPDITSKK 181

Query: 181 EDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA- 240
           EDKAVQSGYDSFGNP+K YSGIRENKLLDSRVRRCQSSLS+YSVSSKRTYLPDDSLCQA 
Sbjct: 182 EDKAVQSGYDSFGNPMKGYSGIRENKLLDSRVRRCQSSLSYYSVSSKRTYLPDDSLCQAV 241

Query: 241 ------------FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 300
                       FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD
Sbjct: 242 RPCLSQPMSMIEFARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSD 301

Query: 301 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPY 360
           PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDM LDNPFHVEGLKEFSGPY
Sbjct: 302 PPLSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMKLDNPFHVEGLKEFSGPY 361

Query: 361 STMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMH 420
           STMVEVPSIYRD+QKLIEVEHLLQDFRSLISKLEEVDP KLNHEEKLAFWINVHNSLMMH
Sbjct: 362 STMVEVPSIYRDNQKLIEVEHLLQDFRSLISKLEEVDPMKLNHEEKLAFWINVHNSLMMH 421

Query: 421 AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSK 480
           AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIP KSK
Sbjct: 422 AYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPLKSK 481

Query: 481 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 540
           FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR
Sbjct: 482 FKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIR 541

Query: 541 KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNF 599
           KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSV KCQNGKSRKNIEWIPHNF
Sbjct: 542 KDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVAKCQNGKSRKNIEWIPHNF 601

BLAST of Carg05249-RA vs. ExPASy TrEMBL
Match: A0A6J1KWZ8 (uncharacterized protein LOC111497149 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111497149 PE=4 SV=1)

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 579/607 (95.39%), Postives = 586/607 (96.54%), Query Frame = 0

Query: 5   ETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTE 64
           ETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTE
Sbjct: 2   ETDGGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTE 61

Query: 65  KKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAKPATE 124
           KKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQD TSSD E+AKPATE
Sbjct: 62  KKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDGTSSDIELAKPATE 121

Query: 125 LIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSKKEDKA 184
           LIKEITVLELEVSHLEQYLLSLYRKAFDGQI+STSP+NSP+SKYMMNCIPDITSKKEDKA
Sbjct: 122 LIKEITVLELEVSHLEQYLLSLYRKAFDGQISSTSPINSPRSKYMMNCIPDITSKKEDKA 181

Query: 185 VQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA----- 244
           VQSGYDSFGNP+K YSGIRENKLLDSRVRRCQSSLS+YSVSSKRTYLPDDSLCQA     
Sbjct: 182 VQSGYDSFGNPMKGYSGIRENKLLDSRVRRCQSSLSYYSVSSKRTYLPDDSLCQAVRPCL 241

Query: 245 --------FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLS 304
                   FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLS
Sbjct: 242 SQPMSMIEFARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLS 301

Query: 305 HHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMV 364
           HHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDM LDNPFHVEGLKEFSGPYSTMV
Sbjct: 302 HHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMKLDNPFHVEGLKEFSGPYSTMV 361

Query: 365 EVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLA 424
           EVPSIYRD+QKLIEVEHLLQDFRSLISKLEEVDP KLNHEEKLAFWINVHNSLMMHAYLA
Sbjct: 362 EVPSIYRDNQKLIEVEHLLQDFRSLISKLEEVDPMKLNHEEKLAFWINVHNSLMMHAYLA 421

Query: 425 YGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNG 484
           YGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIP KSKFKNG
Sbjct: 422 YGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPLKSKFKNG 481

Query: 485 DKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKK 544
           DKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKK
Sbjct: 482 DKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKK 541

Query: 545 VILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQNGKSRKNIEWIPHNFGFRY 599
           VILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSV KCQNGKSRKNIEWIPHNF FRY
Sbjct: 542 VILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVAKCQNGKSRKNIEWIPHNFSFRY 601

BLAST of Carg05249-RA vs. TAIR 10
Match: AT5G66600.1 (Protein of unknown function, DUF547 )

HSP 1 Score: 585.9 bits (1509), Expect = 3.7e-167
Identity = 335/615 (54.47%), Postives = 422/615 (68.62%), Query Frame = 0

Query: 8   GGAKMLQNRQIPLHKHSKS-FLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTEKK 67
           GG +ML  R +  HK SKS      K    ++  ++S EAS R+KLD     ES + +  
Sbjct: 8   GGGRMLDLRIVQNHKRSKSASFPEKKRVEGDKTSNSSHEASQRMKLDMGRSNESKHNQYH 67

Query: 68  SFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQD--NTSSDTEVAKPATE 127
           S    N  +SLKQEI  LE RLQDQFKVR +LEKALGY+ +       ++D  + KPAT+
Sbjct: 68  S----NTETSLKQEITHLETRLQDQFKVRCALEKALGYRTASSYVLTETNDIAMPKPATD 127

Query: 128 LIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTS-----------PMNSPKSKYMMNCI 187
           LIK++ VLE+EV HLEQYLLSLYRKAF+ QI+S S           P+ +P+ +   +  
Sbjct: 128 LIKDVAVLEMEVIHLEQYLLSLYRKAFEQQISSVSPNLENKKPKSPPVTTPRRRLDFSED 187

Query: 188 PDITSKKEDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPD 247
            D  SK +   V    D+     K      +   +D   RR  S  S +     R   P+
Sbjct: 188 DDTPSKTDQHTVPLLDDNQNQSKKTEIAAVDRDQMDPSFRRSHSQRSAF---GSRKASPE 247

Query: 248 DSLCQA---------FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCK 307
           DS  +A         + +N   NL SLA++LGT+I DH+PE+ N+LSE M+KC+S IYCK
Sbjct: 248 DSWGKASRSCHSQPLYVQN-GDNLISLAEHLGTRISDHVPETPNKLSEGMVKCMSEIYCK 307

Query: 308 LSDPP-LSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEF 367
           L++PP + H GLSSP SS S  SAFSP DQ+D  SPGF N+SSFD+ LDN FHVEG K+F
Sbjct: 308 LAEPPSVLHRGLSSPNSSLSS-SAFSPSDQYDTSSPGFGNSSSFDVRLDNSFHVEGEKDF 367

Query: 368 SGPYSTMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNS 427
           SGPYS++VEV  IYRD++K  EVE LLQ+F+SLIS+LEEVDPRKL HEEKLAFWINVHN+
Sbjct: 368 SGPYSSIVEVLCIYRDAKKASEVEDLLQNFKSLISRLEEVDPRKLKHEEKLAFWINVHNA 427

Query: 428 LMMHAYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIP 487
           L+MHA+LAYGIP NN+K+V +LLKAAYNIGG TIS + IQSSILGC++  PGQWL LL  
Sbjct: 428 LVMHAFLAYGIPQNNVKRVLLLLKAAYNIGGHTISAEAIQSSILGCKMSHPGQWLRLLFA 487

Query: 488 SKSKFKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRAT 547
           S+ KFK GD+R AY ID  EPLLHFAL +G HSDPAVRVYTPKR+ QELET+KEEYIR  
Sbjct: 488 SR-KFKAGDERLAYAIDHPEPLLHFALTSGSHSDPAVRVYTPKRIQQELETSKEEYIRMN 547

Query: 548 FGIRKDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQN--GKSRKNIE 597
             IRK ++++LPK++E+FAK+S LC AG  EM+ +S+PES RK V +CQ+   K RK I+
Sbjct: 548 LSIRK-QRILLPKLVETFAKDSGLCPAGLTEMVNRSIPESSRKCVKRCQSSTSKPRKTID 607

BLAST of Carg05249-RA vs. TAIR 10
Match: AT5G66600.3 (Protein of unknown function, DUF547 )

HSP 1 Score: 585.9 bits (1509), Expect = 3.7e-167
Identity = 335/615 (54.47%), Postives = 422/615 (68.62%), Query Frame = 0

Query: 8   GGAKMLQNRQIPLHKHSKS-FLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTEKK 67
           GG +ML  R +  HK SKS      K    ++  ++S EAS R+KLD     ES + +  
Sbjct: 8   GGGRMLDLRIVQNHKRSKSASFPEKKRVEGDKTSNSSHEASQRMKLDMGRSNESKHNQYH 67

Query: 68  SFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQD--NTSSDTEVAKPATE 127
           S    N  +SLKQEI  LE RLQDQFKVR +LEKALGY+ +       ++D  + KPAT+
Sbjct: 68  S----NTETSLKQEITHLETRLQDQFKVRCALEKALGYRTASSYVLTETNDIAMPKPATD 127

Query: 128 LIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTS-----------PMNSPKSKYMMNCI 187
           LIK++ VLE+EV HLEQYLLSLYRKAF+ QI+S S           P+ +P+ +   +  
Sbjct: 128 LIKDVAVLEMEVIHLEQYLLSLYRKAFEQQISSVSPNLENKKPKSPPVTTPRRRLDFSED 187

Query: 188 PDITSKKEDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPD 247
            D  SK +   V    D+     K      +   +D   RR  S  S +     R   P+
Sbjct: 188 DDTPSKTDQHTVPLLDDNQNQSKKTEIAAVDRDQMDPSFRRSHSQRSAF---GSRKASPE 247

Query: 248 DSLCQA---------FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCK 307
           DS  +A         + +N   NL SLA++LGT+I DH+PE+ N+LSE M+KC+S IYCK
Sbjct: 248 DSWGKASRSCHSQPLYVQN-GDNLISLAEHLGTRISDHVPETPNKLSEGMVKCMSEIYCK 307

Query: 308 LSDPP-LSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEF 367
           L++PP + H GLSSP SS S  SAFSP DQ+D  SPGF N+SSFD+ LDN FHVEG K+F
Sbjct: 308 LAEPPSVLHRGLSSPNSSLSS-SAFSPSDQYDTSSPGFGNSSSFDVRLDNSFHVEGEKDF 367

Query: 368 SGPYSTMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNS 427
           SGPYS++VEV  IYRD++K  EVE LLQ+F+SLIS+LEEVDPRKL HEEKLAFWINVHN+
Sbjct: 368 SGPYSSIVEVLCIYRDAKKASEVEDLLQNFKSLISRLEEVDPRKLKHEEKLAFWINVHNA 427

Query: 428 LMMHAYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIP 487
           L+MHA+LAYGIP NN+K+V +LLKAAYNIGG TIS + IQSSILGC++  PGQWL LL  
Sbjct: 428 LVMHAFLAYGIPQNNVKRVLLLLKAAYNIGGHTISAEAIQSSILGCKMSHPGQWLRLLFA 487

Query: 488 SKSKFKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRAT 547
           S+ KFK GD+R AY ID  EPLLHFAL +G HSDPAVRVYTPKR+ QELET+KEEYIR  
Sbjct: 488 SR-KFKAGDERLAYAIDHPEPLLHFALTSGSHSDPAVRVYTPKRIQQELETSKEEYIRMN 547

Query: 548 FGIRKDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQN--GKSRKNIE 597
             IRK ++++LPK++E+FAK+S LC AG  EM+ +S+PES RK V +CQ+   K RK I+
Sbjct: 548 LSIRK-QRILLPKLVETFAKDSGLCPAGLTEMVNRSIPESSRKCVKRCQSSTSKPRKTID 607

BLAST of Carg05249-RA vs. TAIR 10
Match: AT5G66600.4 (Protein of unknown function, DUF547 )

HSP 1 Score: 580.5 bits (1495), Expect = 1.6e-165
Identity = 336/630 (53.33%), Postives = 421/630 (66.83%), Query Frame = 0

Query: 8   GGAKMLQNRQIPLHKHSKSFLDMVKDTNKEEHLDNSF----------------EASNRVK 67
           GG +ML  R +  HK SK    +      E+  D SF                EAS R+K
Sbjct: 8   GGGRMLDLRIVQNHKRSKRSSVVTVFCGPEQDNDASFPEKKRVEGDKTSNSSHEASQRMK 67

Query: 68  LDTSSFKESMNTEKKSFPKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQD- 127
           LD     ES + +  S    N  +SLKQEI  LE RLQDQFKVR +LEKALGY+ +    
Sbjct: 68  LDMGRSNESKHNQYHS----NTETSLKQEITHLETRLQDQFKVRCALEKALGYRTASSYV 127

Query: 128 -NTSSDTEVAKPATELIKEITVLELEVSHLEQYLLSLYRKAFDGQIASTS---------- 187
              ++D  + KPAT+LIK++ VLE+EV HLEQYLLSLYRKAF+ QI+S S          
Sbjct: 128 LTETNDIAMPKPATDLIKDVAVLEMEVIHLEQYLLSLYRKAFEQQISSVSPNLENKKPKS 187

Query: 188 -PMNSPKSKYMMNCIPDITSKKEDKAVQSGYDSFGNPVKEYSGIRENKLLDSRVRRCQSS 247
            P+ +P+ +   +   D  SK +   V    D+     K      +   +D   RR  S 
Sbjct: 188 PPVTTPRRRLDFSEDDDTPSKTDQHTVPLLDDNQNQSKKTEIAAVDRDQMDPSFRRSHSQ 247

Query: 248 LSHYSVSSKRTYLPDDSLCQA---------FARNASSNLTSLADYLGTQILDHIPESANR 307
            S +     R   P+DS  +A         + +N   NL SLA++LGT+I DH+PE+ N+
Sbjct: 248 RSAF---GSRKASPEDSWGKASRSCHSQPLYVQN-GDNLISLAEHLGTRISDHVPETPNK 307

Query: 308 LSEDMIKCISAIYCKLSDPP-LSHHGLSSPVSSSSPISAFSPQDQFDMLSPGFRNNSSFD 367
           LSE M+KC+S IYCKL++PP + H GLSSP SS S  SAFSP DQ+D  SPGF N+SSFD
Sbjct: 308 LSEGMVKCMSEIYCKLAEPPSVLHRGLSSPNSSLSS-SAFSPSDQYDTSSPGFGNSSSFD 367

Query: 368 MMLDNPFHVEGLKEFSGPYSTMVEVPSIYRDSQKLIEVEHLLQDFRSLISKLEEVDPRKL 427
           + LDN FHVEG K+FSGPYS++VEV  IYRD++K  EVE LLQ+F+SLIS+LEEVDPRKL
Sbjct: 368 VRLDNSFHVEGEKDFSGPYSSIVEVLCIYRDAKKASEVEDLLQNFKSLISRLEEVDPRKL 427

Query: 428 NHEEKLAFWINVHNSLMMHAYLAYGIPHNNMKKVFVLLKAAYNIGGQTISVDTIQSSILG 487
            HEEKLAFWINVHN+L+MHA+LAYGIP NN+K+V +LLKAAYNIGG TIS + IQSSILG
Sbjct: 428 KHEEKLAFWINVHNALVMHAFLAYGIPQNNVKRVLLLLKAAYNIGGHTISAEAIQSSILG 487

Query: 488 CRVPRPGQWLSLLIPSKSKFKNGDKRQAYKIDRSEPLLHFALCTGCHSDPAVRVYTPKRV 547
           C++  PGQWL LL  S+ KFK GD+R AY ID  EPLLHFAL +G HSDPAVRVYTPKR+
Sbjct: 488 CKMSHPGQWLRLLFASR-KFKAGDERLAYAIDHPEPLLHFALTSGSHSDPAVRVYTPKRI 547

Query: 548 LQELETAKEEYIRATFGIRKDKKVILPKMIESFAKESRLCTAGTMEMIQKSLPESLRKSV 597
            QELET+KEEYIR    IRK ++++LPK++E+FAK+S LC AG  EM+ +S+PES RK V
Sbjct: 548 QQELETSKEEYIRMNLSIRK-QRILLPKLVETFAKDSGLCPAGLTEMVNRSIPESSRKCV 607

BLAST of Carg05249-RA vs. TAIR 10
Match: AT5G66600.2 (Protein of unknown function, DUF547 )

HSP 1 Score: 574.7 bits (1480), Expect = 8.6e-164
Identity = 329/602 (54.65%), Postives = 416/602 (69.10%), Query Frame = 0

Query: 20  LHKHSKSFLDMVKDTNKEEHLDNSFEASNRVKLDTSSFKESMNTEKKSFPKTNIHSSLKQ 79
           L  +S SF +  K    ++  ++S EAS R+KLD     ES + +  S    N  +SLKQ
Sbjct: 2   LSLNSASFPEK-KRVEGDKTSNSSHEASQRMKLDMGRSNESKHNQYHS----NTETSLKQ 61

Query: 80  EILQLEKRLQDQFKVRTSLEKALGYKPSCQD--NTSSDTEVAKPATELIKEITVLELEVS 139
           EI  LE RLQDQFKVR +LEKALGY+ +       ++D  + KPAT+LIK++ VLE+EV 
Sbjct: 62  EITHLETRLQDQFKVRCALEKALGYRTASSYVLTETNDIAMPKPATDLIKDVAVLEMEVI 121

Query: 140 HLEQYLLSLYRKAFDGQIASTS-----------PMNSPKSKYMMNCIPDITSKKEDKAVQ 199
           HLEQYLLSLYRKAF+ QI+S S           P+ +P+ +   +   D  SK +   V 
Sbjct: 122 HLEQYLLSLYRKAFEQQISSVSPNLENKKPKSPPVTTPRRRLDFSEDDDTPSKTDQHTVP 181

Query: 200 SGYDSFGNPVKEYSGIRENKLLDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQA------- 259
              D+     K      +   +D   RR  S  S +     R   P+DS  +A       
Sbjct: 182 LLDDNQNQSKKTEIAAVDRDQMDPSFRRSHSQRSAF---GSRKASPEDSWGKASRSCHSQ 241

Query: 260 --FARNASSNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPP-LSHHGLS 319
             + +N   NL SLA++LGT+I DH+PE+ N+LSE M+KC+S IYCKL++PP + H GLS
Sbjct: 242 PLYVQN-GDNLISLAEHLGTRISDHVPETPNKLSEGMVKCMSEIYCKLAEPPSVLHRGLS 301

Query: 320 SPVSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMVEVPSI 379
           SP SS S  SAFSP DQ+D  SPGF N+SSFD+ LDN FHVEG K+FSGPYS++VEV  I
Sbjct: 302 SPNSSLSS-SAFSPSDQYDTSSPGFGNSSSFDVRLDNSFHVEGEKDFSGPYSSIVEVLCI 361

Query: 380 YRDSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLAYGIPH 439
           YRD++K  EVE LLQ+F+SLIS+LEEVDPRKL HEEKLAFWINVHN+L+MHA+LAYGIP 
Sbjct: 362 YRDAKKASEVEDLLQNFKSLISRLEEVDPRKLKHEEKLAFWINVHNALVMHAFLAYGIPQ 421

Query: 440 NNMKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNGDKRQA 499
           NN+K+V +LLKAAYNIGG TIS + IQSSILGC++  PGQWL LL  S+ KFK GD+R A
Sbjct: 422 NNVKRVLLLLKAAYNIGGHTISAEAIQSSILGCKMSHPGQWLRLLFASR-KFKAGDERLA 481

Query: 500 YKIDRSEPLLHFALCTGCHSDPAVRVYTPKRVLQELETAKEEYIRATFGIRKDKKVILPK 559
           Y ID  EPLLHFAL +G HSDPAVRVYTPKR+ QELET+KEEYIR    IRK ++++LPK
Sbjct: 482 YAIDHPEPLLHFALTSGSHSDPAVRVYTPKRIQQELETSKEEYIRMNLSIRK-QRILLPK 541

Query: 560 MIESFAKESRLCTAGTMEMIQKSLPESLRKSVTKCQN--GKSRKNIEWIPHNFGFRYLIC 597
           ++E+FAK+S LC AG  EM+ +S+PES RK V +CQ+   K RK I+WIPH+F FRYLI 
Sbjct: 542 LVETFAKDSGLCPAGLTEMVNRSIPESSRKCVKRCQSSTSKPRKTIDWIPHSFTFRYLIL 591

BLAST of Carg05249-RA vs. TAIR 10
Match: AT2G23700.1 (Protein of unknown function, DUF547 )

HSP 1 Score: 515.4 bits (1326), Expect = 6.2e-146
Identity = 327/720 (45.42%), Postives = 417/720 (57.92%), Query Frame = 0

Query: 11  KMLQNRQIPLHKHSKS-FLDMVKDTNKEEHLDNSFEASNRVKLDTSSF-KESMNTEKKSF 70
           KML+ R    HK SKS  +   K    E  +D+S +AS R+KLD      +S   +K   
Sbjct: 7   KMLRQR----HKRSKSCTVPEKKKLEDENSIDSSLDASQRLKLDLPRCGDKSFEMKKDLS 66

Query: 71  PKTNIHSSLKQEILQLEKRLQDQFKVRTSLEKALGYKPSCQDNTSSDTEVAKPATELIKE 130
           P     SSLKQEI +LEKRLQ+QF VR +LEKALGYK   +D     T   KP TELIKE
Sbjct: 67  PDVKFKSSLKQEIQELEKRLQNQFDVRGALEKALGYKTPSRDIKGDST--PKPPTELIKE 126

Query: 131 ITVLELEVSHLEQYLLSLYRKAFDGQIASTSPMNSPKSKYMMNCIPDITSK--------- 190
           I VLELEVSHLEQYLLSLYRKAFD Q +S SP   P SK   +C P  T +         
Sbjct: 127 IAVLELEVSHLEQYLLSLYRKAFDQQTSSVSP---PTSKQQSSCSPKSTLRGKRLDFSRT 186

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 187 PESRCFSFDNRLKSPRLVEKELESPNLRCRQESLATQPRCFSFDNRLKEPSSAGRQCNQE 246

Query: 251 --------------------------KEDKAVQSGYDSFGNPVKE-YSGIR--------- 310
                                     +ED  + S   SF N VKE  SG+R         
Sbjct: 247 VSRIDSRSFSFDNRVKEPGSAARHFNQEDSRIDSQCVSFDNRVKEPVSGVRQFDQESSRI 306

Query: 311 -------ENKL----------LDSRVRRCQSSLSHYSVSSKRTYLPDDSLCQAFARNAS- 370
                  +N+L          +DS VRRCQSSL+  S  + R   P+DS+    ++  S 
Sbjct: 307 DSRCFSFDNRLKDQCFIEKEDIDSCVRRCQSSLNQRSTFNNRISPPEDSVFACHSQPLSI 366

Query: 371 -------SNLTSLADYLGTQILDHIPESANRLSEDMIKCISAIYCKLSDPPLSHHGLSSP 430
                  SN  SLA+++GT+I DHI  + N+LSE+MIKC SAIY KL+DPP  +HG SSP
Sbjct: 367 HEYIQNGSNDASLAEHMGTRISDHIFMTPNKLSEEMIKCASAIYSKLADPPSINHGFSSP 426

Query: 431 VSSSSPISAFSPQDQFDMLSPGFRNNSSFDMMLDNPFHVEGLKEFSGPYSTMVEVPSIYR 490
            SS S  S FSPQDQ+DM SP FR NSSFD             EFSGPYS+M+EV  I+R
Sbjct: 427 SSSPSSTSEFSPQDQYDMWSPSFRKNSSFDDQF----------EFSGPYSSMIEVSHIHR 486

Query: 491 DSQKLIEVEHLLQDFRSLISKLEEVDPRKLNHEEKLAFWINVHNSLMMHAYLAYGIPHNN 550
            ++K  +++ + ++F  L+ +LE VDPRKL H+EKLAFWINVHN+L+MH +LA GIP NN
Sbjct: 487 -NRKRRDLDLMNRNFSLLLKQLESVDPRKLTHQEKLAFWINVHNALVMHTFLANGIPQNN 546

Query: 551 MKKVFVLLKAAYNIGGQTISVDTIQSSILGCRVPRPGQWLSLLIPSKSKFKNGDKRQAYK 598
            K+  +L K AY IGG+ +S++ IQS IL  ++PRPGQWL LL+  K KF+ GD+ Q Y 
Sbjct: 547 GKRFLLLSKPAYKIGGRMVSLEAIQSYILRIKMPRPGQWLKLLLIPK-KFRTGDEHQEYS 606

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027088.10.0e+00100.00hypothetical protein SDJN02_11098 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022962712.10.0e+0097.71uncharacterized protein LOC111463123 isoform X1 [Cucurbita moschata] >XP_0229627... [more]
XP_022962714.10.0e+0097.69uncharacterized protein LOC111463123 isoform X2 [Cucurbita moschata] >XP_0229627... [more]
KAG6595073.10.0e+0096.73hypothetical protein SDJN03_11626, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023003592.10.0e+0095.42uncharacterized protein LOC111497149 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1HFK60.0e+0097.71uncharacterized protein LOC111463123 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1HHW50.0e+0097.69uncharacterized protein LOC111463123 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KN070.0e+0095.42uncharacterized protein LOC111497149 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1KPN70.0e+0095.42uncharacterized protein LOC111497149 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1KWZ80.0e+0095.39uncharacterized protein LOC111497149 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G66600.13.7e-16754.47Protein of unknown function, DUF547 [more]
AT5G66600.33.7e-16754.47Protein of unknown function, DUF547 [more]
AT5G66600.41.6e-16553.33Protein of unknown function, DUF547 [more]
AT5G66600.28.6e-16454.65Protein of unknown function, DUF547 [more]
AT2G23700.16.2e-14645.42Protein of unknown function, DUF547 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006869Domain of unknown function DUF547PFAMPF04784DUF547coord: 385..520
e-value: 4.3E-42
score: 143.1
IPR025757Ternary complex factor MIP1, leucine-zipperPFAMPF14389Lzipper-MIP1coord: 72..152
e-value: 2.9E-25
score: 88.4
NoneNo IPR availablePANTHERPTHR23054:SF18BNAA07G12450D PROTEINcoord: 19..597
NoneNo IPR availablePANTHERPTHR23054UNCHARACTERIZEDcoord: 19..597

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg05249Carg05249gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg05249-RA:11Carg05249-RA:11exon
Carg05249-RA:10Carg05249-RA:10exon
Carg05249-RA:9Carg05249-RA:9exon
Carg05249-RA:8Carg05249-RA:8exon
Carg05249-RA:7Carg05249-RA:7exon
Carg05249-RA:6Carg05249-RA:6exon
Carg05249-RA:5Carg05249-RA:5exon
Carg05249-RA:4Carg05249-RA:4exon
Carg05249-RA:3Carg05249-RA:3exon
Carg05249-RA:2Carg05249-RA:2exon
Carg05249-RA:1Carg05249-RA:1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg05249-RA:three_prime_utrCarg05249-RA:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg05249-RA:cdsCarg05249-RA:cds_11CDS
Carg05249-RA:cdsCarg05249-RA:cds_10CDS
Carg05249-RA:cdsCarg05249-RA:cds_9CDS
Carg05249-RA:cdsCarg05249-RA:cds_8CDS
Carg05249-RA:cdsCarg05249-RA:cds_7CDS
Carg05249-RA:cdsCarg05249-RA:cds_6CDS
Carg05249-RA:cdsCarg05249-RA:cds_5CDS
Carg05249-RA:cdsCarg05249-RA:cds_4CDS
Carg05249-RA:cdsCarg05249-RA:cds_3CDS
Carg05249-RA:cdsCarg05249-RA:cds_2CDS
Carg05249-RA:cdsCarg05249-RA:cdsCDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg05249-RA:five_prime_utrCarg05249-RA:five_prime_utrfive_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg05249-RACarg05249-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane