Carg07931-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg07931-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Carg_Chr07: 1764334 .. 1767324 (+)
Sequence length	1397
RNA-Seq Expression	Carg07931-RA
Synteny	Carg07931-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCATTATTATTTAATTAAGCCGCCGGAGACGGAGGGAAATTAATTAAGGAAACAATAAAGAAAACTGCTCGGATCGAGAGTGGAGAGGAAGAAGCGAAACCAATCTCGGCGTCGGAGTTCATCAAGATTTCCCATTACCGACTCGGAGGATTTGGACTGTGGAGACGAAGACACGAACCGATACTCTGCTCTGAAATTCCAGATCTTGCTCTCCTCCTTCCATGGCGAAGCACAGGCAACCTCGATTTCCTTCTCGGAAGTCGTCTTCTTCTTCTACTCTCATATTTACCTTGCTCATTATGTTCACGTTCGTCATTCTGATTCTTCTTGCCCTTGGAATCCTCTCGATCCCTGGAAATTCTGGCGCGCCCAAGGCTCATGATCTGAGCTCGATCGTGCGGAAAACTTCCGATGAGTATGTTATTATCTGTGTTCGATTTCAATTTTTTTGTTCCGTATGGGGTTGATTTTAAATTGATTGTTTGATTTTGTTTTAGAGTTGACGAGGAGAAAGGAGAGCAGTGGGCTGAAGTGATCTCATGGGAACCTAGAGCCTTCGTTTATCACAATTTTCTGGTATTCATTCCCCCCCGCCCGATCTATGACGAGGGATTTTTGTTTTATGTTCGTTCGTCGCTCTCTATGCAATTTGTGTATGGAATTATGTGAACTTGGTTGCTGTATGCTGATGATACGTGTTGGATGCGTGCATATGCCAATGAAAACCAAGGGATTATGTTTACGTTTCTCTTAGAAATCCTTCTTCTCTTATGTTATGCTGAGAGGGGTTGAGGATGATTCGCATCTGTATTTGTCTTTAGATTGCTTTAAGGAATTTCTGAATTGGTTTACTTTTTAATTGCTCAGACAAAGGAGGAATGTGAGTACCTAATCAGCCTTGCCAAGCCTCACATGCAAAAGTCTTCCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGGTCTGAAATTCTCTTCCCCAGAATTCCCTTTTGTTAGTAATGCTTGATCTCTAATGTAGAAGCCTCCACGACCTCTCTTTTACAGAGTTCGTACTAGCTCTGGAACATTTTTGCCCAGAGGACGTGATAAGATTATTAGAAACATCGAGAAAAGGATTGCTGATTTCAGCTTCGTACCCGTAGGTATATATTCTTGAGCCTATTTTCCTCTTCCTGGTTTCTTCCCACTACATCCAGCATTGTTCTTCTTTTATTTTATTATGCATGGCTAAACTAAACGTTACAATCTTTGATTTATTTCCTACTGCACCTATCGTTGTGATTGAACTTTTCGCCTATAAACCAGCATTCCCAATCACACAATCGTGCGCAAGACCGTTCAAATAGACACAACTGCCGTGCTAAGCTTCACATGGATGAGGAAGATTTTTAACCGTGGTTGAAAAGTCGAATAACGAATATTCACTCAAATGTAGGAACACCAAAATGAATCAGATATTTAACCTATGAGCTTAACCACCCATTGCCTCTTTTTAAGAATGAAAGAGAATTGGGTTCGCCTGATCAGATGTGCCAATTTTTGTTTATTATAAGTAGATATTGTCTTGTTATCTCCTCTTTTCATATTTTGTTACTGAAGCCCACTCACAATATATGTAGAGCATGGAGAAGGACTTCAGGTTCTTCATTATGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGATGAATACAATACAAAGAATGGAGGTCAACGTATAGCAACAATGCTTATGTATCTGTAAGTAGTAATTTATATCCACTATGCTTGGGACTCTAACCAGGTGGTGTTATATCATGTGTGGTTCTCCCATTATTTTAACAACTTCTATCATAATTCAGCTCGGATGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTACCTTGGTGGGATGAGCTTTCAGATTGTGGGAAGAAAGGACTTTCTGTTAAACCGAAGAGGGGCGATGCGTTACTTTTCTGGAGCATGAAGCCTGATGCCTCTCTAGATCCATCAAGTCTGCATGGTTAGATTTGGATGCTCTATAACTTATAATCTACCATTCATTTTACAAAGCATTATAGGTTTCAGTGATGGGCATTTATTTTGTTATTGGGATTTTTCACATCTCTCGTGTCGAAAGTAATGGGTTTTCTTGATAAAATGATGACGTTTCTTCTGATGATCGTTTATAGGTGGTTGCCCTGTTATCAAGGGGAACAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATACAAAGCTTGAGTGGATGCGACTAAGGTACTTTCCATGCCCTAAACCATGCACTAGAATCGTAATCAAACGAACACCTAAAACAACACTACAGCTGATTTTTCATGATTCTCAAAAAACCATAAACCAAATGGTTATCAAATGGGAAATTTCTTTTTGCCTCTCAACATAGAACAGCTTGAATTGCTGAATCACCTTCACATTAAGCACGCTGTAATTTGATTGAGTCTGGTTTTGAACTCCCCACCCAAATCCCGCATATTGAGCGCAGCTGATATGCGGAGCTAGGCGATCATATCATGCCATAAAACGGGAAAAAAGAACAAAGGAATTCTTTCCTTCTGCTGCGTTGTTTTTTCTTGTTATTGCTGAAAGAACTATTCCTAAAGATGTGGTGATGCTTCATTGTTTGGCAGGTGTGGTTGAATTTTCTTGTTATTGCTGAAAATCAGAGATGCATTTGCTGTAGGGAAAACCACCATTTCTATAATTTAACCTATGAATTTTGCTGTGCTTAATGTTTTAATTAGGAGCTTACATTGTGATTGATTTCTTGTTTATGGCCACGGAGAGAATAAGAGTTCATATTTTTGCTAGAAATGCCACTTCCATATGTAAAAAGAACAAAGATTTTAAGCTTGATGTACAGCTCCCTTGCTGCAACATTCTCGTTTATTTTCTATTTGATATTACTTTATATTCTGTGTTCATTTCTT

mRNA sequence

TCATTATTATTTAATTAAGCCGCCGGAGACGGAGGGAAATTAATTAAGGAAACAATAAAGAAAACTGCTCGGATCGAGAGTGGAGAGGAAGAAGCGAAACCAATCTCGGCGTCGGAGTTCATCAAGATTTCCCATTACCGACTCGGAGGATTTGGACTGTGGAGACGAAGACACGAACCGATACTCTGCTCTGAAATTCCAGATCTTGCTCTCCTCCTTCCATGGCGAAGCACAGGCAACCTCGATTTCCTTCTCGGAAGTCGTCTTCTTCTTCTACTCTCATATTTACCTTGCTCATTATGTTCACGTTCGTCATTCTGATTCTTCTTGCCCTTGGAATCCTCTCGATCCCTGGAAATTCTGGCGCGCCCAAGGCTCATGATCTGAGCTCGATCGTGCGGAAAACTTCCGATGAAGTTGACGAGGAGAAAGGAGAGCAGTGGGCTGAAGTGATCTCATGGGAACCTAGAGCCTTCGTTTATCACAATTTTCTGACAAAGGAGGAATGTGAGTACCTAATCAGCCTTGCCAAGCCTCACATGCAAAAGTCTTCCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGAGTTCGTACTAGCTCTGGAACATTTTTGCCCAGAGGACGTGATAAGATTATTAGAAACATCGAGAAAAGGATTGCTGATTTCAGCTTCGTACCCGTAGAGCATGGAGAAGGACTTCAGGTTCTTCATTATGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGATGAATACAATACAAAGAATGGAGGTCAACGTATAGCAACAATGCTTATGTATCTCTCGGATGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTACCTTGGTGGGATGAGCTTTCAGATTGTGGGAAGAAAGGACTTTCTGTTAAACCGAAGAGGGGCGATGCGTTACTTTTCTGGAGCATGAAGCCTGATGCCTCTCTAGATCCATCAAGTCTGCATGGTGGTTGCCCTGTTATCAAGGGGAACAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATACAAAGCTTGAGTGGATGCGACTAAGGTGTGGTTGAATTTTCTTGTTATTGCTGAAAATCAGAGATGCATTTGCTGTAGGGAAAACCACCATTTCTATAATTTAACCTATGAATTTTGCTGTGCTTAATGTTTTAATTAGGAGCTTACATTGTGATTGATTTCTTGTTTATGGCCACGGAGAGAATAAGAGTTCATATTTTTGCTAGAAATGCCACTTCCATATGTAAAAAGAACAAAGATTTTAAGCTTGATGTACAGCTCCCTTGCTGCAACATTCTCGTTTATTTTCTATTTGATATTACTTTATATTCTGTGTTCATTTCTT

Coding sequence (CDS)

ATGGCGAAGCACAGGCAACCTCGATTTCCTTCTCGGAAGTCGTCTTCTTCTTCTACTCTCATATTTACCTTGCTCATTATGTTCACGTTCGTCATTCTGATTCTTCTTGCCCTTGGAATCCTCTCGATCCCTGGAAATTCTGGCGCGCCCAAGGCTCATGATCTGAGCTCGATCGTGCGGAAAACTTCCGATGAAGTTGACGAGGAGAAAGGAGAGCAGTGGGCTGAAGTGATCTCATGGGAACCTAGAGCCTTCGTTTATCACAATTTTCTGACAAAGGAGGAATGTGAGTACCTAATCAGCCTTGCCAAGCCTCACATGCAAAAGTCTTCCGTTGTTGACAGTGAAACTGGAAAGAGCAAAGATAGCAGAGTTCGTACTAGCTCTGGAACATTTTTGCCCAGAGGACGTGATAAGATTATTAGAAACATCGAGAAAAGGATTGCTGATTTCAGCTTCGTACCCGTAGAGCATGGAGAAGGACTTCAGGTTCTTCATTATGAAGTGGGACAGAAATATGAACCTCATTTTGATTACTTCCTTGATGAATACAATACAAAGAATGGAGGTCAACGTATAGCAACAATGCTTATGTATCTCTCGGATGTTGAAGAAGGAGGCGAGACAGTGTTCCCTGCTGCCAAAGGAAACTTTAGTTCTGTACCTTGGTGGGATGAGCTTTCAGATTGTGGGAAGAAAGGACTTTCTGTTAAACCGAAGAGGGGCGATGCGTTACTTTTCTGGAGCATGAAGCCTGATGCCTCTCTAGATCCATCAAGTCTGCATGGTGGTTGCCCTGTTATCAAGGGGAACAAATGGTCTGCTACTAAATGGATGCGAGTAGAAGAATACAAAGCTTGA

Protein sequence

MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVRKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKSKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYFLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA

Homology

[Close]

BLAST of Carg07931-RA vs. NCBI nr
Match: XP_022926539.1 (probable prolyl 4-hydroxylase 10 [Cucurbita moschata] >XP_023517633.1 probable prolyl 4-hydroxylase 10 [Cucurbita pepo subsp. pepo] >KAG7026717.1 putative prolyl 4-hydroxylase 10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 583.2 bits (1502), Expect = 1.2e-162
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR 60
           MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR
Sbjct: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR 60

Query: 61  KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS 120
           KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS
Sbjct: 61  KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS 120

Query: 121 KDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF 180
           KDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF
Sbjct: 121 KDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF 180

Query: 181 LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK 240
           LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK
Sbjct: 181 LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK 240

Query: 241 RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287
           RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 286

[Close]

BLAST of Carg07931-RA vs. NCBI nr
Match: XP_023003922.1 (probable prolyl 4-hydroxylase 10 [Cucurbita maxima])

HSP 1 Score: 577.8 bits (1488), Expect = 5.2e-161
Identity = 284/286 (99.30%), Postives = 284/286 (99.30%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR 60
           MAKHRQPRF SRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR
Sbjct: 1   MAKHRQPRFSSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR 60

Query: 61  KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS 120
           KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS
Sbjct: 61  KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS 120

Query: 121 KDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF 180
           KDSRVRTSSGTFLPRGRDKIIR IEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF
Sbjct: 121 KDSRVRTSSGTFLPRGRDKIIRTIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF 180

Query: 181 LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK 240
           LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK
Sbjct: 181 LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK 240

Query: 241 RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287
           RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 286

[Close]

BLAST of Carg07931-RA vs. NCBI nr
Match: XP_008440878.1 (PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo])

HSP 1 Score: 553.1 bits (1424), Expect = 1.4e-153
Identity = 269/287 (93.73%), Postives = 280/287 (97.56%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNS-GAPKAHDLSSIV 60
           MAKHRQ RFP+RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNS G+ K HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGK 120
           RKTSD+VDEEKGEQW EVISWEPRAF+YHNFLTKEECEYLISLAKPHMQKS+VVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDK IR IEKRI+DFSF+PVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKP 240
           FLDEYNTKNGGQRIAT+LMYLSDVEEGGETVFPAAKGNFSSVPWW+ELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of Carg07931-RA vs. NCBI nr
Match: XP_004134841.1 (probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prolyl 4-hydroxylase 10 [Cucumis sativus] >KGN48959.1 hypothetical protein Csa_003630 [Cucumis sativus])

HSP 1 Score: 552.4 bits (1422), Expect = 2.3e-153
Identity = 268/287 (93.38%), Postives = 280/287 (97.56%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNS-GAPKAHDLSSIV 60
           MAKHRQ RFP+RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNS G+ K HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGK 120
           RKTSD+VDEEKGEQW EVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKS+VVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDK +R IEKR++DFSF+PVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKP 240
           FLDEYNTKNGGQRIAT+LMYLSDVEEGGETVFPAAKGNFSSVPWW+ELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of Carg07931-RA vs. NCBI nr
Match: XP_038881910.1 (probable prolyl 4-hydroxylase 10 isoform X2 [Benincasa hispida])

HSP 1 Score: 550.8 bits (1418), Expect = 6.8e-153
Identity = 268/286 (93.71%), Postives = 279/286 (97.55%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNS-GAPKAHDLSSIV 60
           MAKHRQ RFP+RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNS G+PK HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGK 120
           RKTSD+VDEE+GEQW EVISWEPRAFVYHNFLTKEECEYLISLA PHMQKS+VVDSETGK
Sbjct: 61  RKTSDDVDEEQGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAMPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDK IR IEKRIADFSF+PVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRIADFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKP 240
           FLDEYNTKNGGQRIAT+LMYLS+VEEGGETVFPAAKGNFSSVPWW+ELS+CGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSEVEEGGETVFPAAKGNFSSVPWWNELSECGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286

[Close]

BLAST of Carg07931-RA vs. ExPASy Swiss-Prot
Match: F4JZ24 (Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 2.7e-128
Identity = 223/288 (77.43%), Postives = 254/288 (88.19%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIP-GNSGAPKAHDLSSIV 60
           MA+ R  R PS + SS STL+F +LIM TFVILILLA GILS+P  N+G+ KA+DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKTSDE--VDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSET 120
           RKT      D+ K E+W E+ISWEPRA VYHNFLTKEEC+YLI LAKPHM+KS+VVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RGRDK IR IEKRI+DF+F+PVEHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSV 240
           DYF+DEYNT+NGGQRIAT+LMYLSDVEEGGETVFPAAKGN+S+VPWW+ELS+CGK GLSV
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSV 241

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286
           KPK GDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV EYK
Sbjct: 242 KPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEYK 288

[Close]

BLAST of Carg07931-RA vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 6.1e-120
Identity = 208/287 (72.47%), Postives = 245/287 (85.37%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR 60
           MAK R  RF +RK S+   ++F +L M T V+L+LLA G+ S+P N+      DLS   R
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRR 60

Query: 61  KTSDEVD--EEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETG 120
             ++  +   ++G+QW EV+SWEPRAFVYHNFL+KEECEYLISLAKPHM KS+VVDSETG
Sbjct: 61  AATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETG 120

Query: 121 KSKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFD 180
           KSKDSRVRTSSGTFL RGRDKII+ IEKRIAD++F+P +HGEGLQVLHYE GQKYEPH+D
Sbjct: 121 KSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYD 180

Query: 181 YFLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVK 240
           YF+DE+NTKNGGQR+ATMLMYLSDVEEGGETVFPAA  NFSSVPW++ELS+CGKKGLSVK
Sbjct: 181 YFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVK 240

Query: 241 PKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286
           P+ GDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V EYK
Sbjct: 241 PRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]

BLAST of Carg07931-RA vs. ExPASy Swiss-Prot
Match: Q24JN5 (Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 8.2e-117
Identity = 204/281 (72.60%), Postives = 240/281 (85.41%), Query Frame = 0


Query: 8   RFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIP-GNSGAPKAHDLSSIVRK--TSD 67
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  N  + K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 68  EVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKSKDSR 127
             +E  GE+W EVISWEPRA VYHNFLT EECE+LISLAKP M KS+VVD +TG SKDSR
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSR 129

Query: 128 VRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYFLDEY 187
           VRTSSGTFL RG D+++  IEKRI+DF+F+PVE+GEGLQVLHY+VGQKYEPH+DYFLDE+
Sbjct: 130 VRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFLDEF 189

Query: 188 NTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPKRGDA 247
           NTKNGGQRIAT+LMYLSDV++GGETVFPAA+GN S+VPWW+ELS CGK+GLSV PK+ DA
Sbjct: 190 NTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDA 249

Query: 248 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286
           LLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V E+K
Sbjct: 250 LLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]

BLAST of Carg07931-RA vs. ExPASy Swiss-Prot
Match: F4JNU8 (Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.1e-110
Identity = 198/288 (68.75%), Postives = 232/288 (80.56%), Query Frame = 0


Query: 3   KHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPG-NSGAPKAHDLSSIV-- 62
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  N  +    DL++IV  
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 63  ---RKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSE 122
              R++  + ++  G++W EVISWEPRAFVYHNFLT EECE+LISLAKP M KS VVD +
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVK 123

Query: 123 TGKSKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPH 182
           TGKS DSRVRTSSGTFL RG D+I+  IE RI+DF+F+P E+GEGLQVLHYEVGQ+YEPH
Sbjct: 124 TGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPH 183

Query: 183 FDYFLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLS 242
            DYF DE+N + GGQRIAT+LMYLSDV+EGGETVFPAAKGN S VPWWDELS CGK+GLS
Sbjct: 184 HDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLS 243

Query: 243 VKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEY 285
           V PK+ DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V EY
Sbjct: 244 VLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]

BLAST of Carg07931-RA vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.9e-65
Identity = 119/209 (56.94%), Postives = 162/209 (77.51%), Query Frame = 0


Query: 78  ISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKSKDSRVRTSSGTFLPRGR 137
           +S +PRAFVY  FLT  EC++LISLAK ++Q+S+V D++ G+S+ S VRTSSGTF+ +G+
Sbjct: 41  VSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVSDVRTSSGTFISKGK 100

Query: 138 DKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYFLDEYNTKNGGQRIATML 197
           D I+  IE +++ ++F+P E+GE LQVL YE GQKY+ HFDYF D+ N   GG RIAT+L
Sbjct: 101 DPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDKVNIARGGHRIATVL 160

Query: 198 MYLSDVEEGGETVFPAAK--GNFSSVPWWDELSDCGKKGLSVKPKRGDALLFWSMKPDAS 257
           +YLS+V +GGETVFP A+     S     D+LSDC KKG++VKPK+G+ALLF++++ DA 
Sbjct: 161 LYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPKKGNALLFFNLQQDAI 220

Query: 258 LDPSSLHGGCPVIKGNKWSATKWMRVEEY 285
            DP SLHGGCPVI+G KWSATKW+ V+ +
Sbjct: 221 PDPFSLHGGCPVIEGEKWSATKWIHVDSF 249

[Close]

BLAST of Carg07931-RA vs. ExPASy TrEMBL
Match: A0A6J1EFG6 (probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111433654 PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.0e-163
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR 60
           MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR
Sbjct: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR 60

Query: 61  KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS 120
           KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS
Sbjct: 61  KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS 120

Query: 121 KDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF 180
           KDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF
Sbjct: 121 KDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF 180

Query: 181 LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK 240
           LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK
Sbjct: 181 LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK 240

Query: 241 RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287
           RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 286

[Close]

BLAST of Carg07931-RA vs. ExPASy TrEMBL
Match: A0A6J1KNY9 (probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111497367 PE=4 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 2.5e-161
Identity = 284/286 (99.30%), Postives = 284/286 (99.30%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR 60
           MAKHRQPRF SRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR
Sbjct: 1   MAKHRQPRFSSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR 60

Query: 61  KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS 120
           KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS
Sbjct: 61  KTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKS 120

Query: 121 KDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF 180
           KDSRVRTSSGTFLPRGRDKIIR IEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF
Sbjct: 121 KDSRVRTSSGTFLPRGRDKIIRTIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYF 180

Query: 181 LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK 240
           LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK
Sbjct: 181 LDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPK 240

Query: 241 RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287
           RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 RGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 286

[Close]

BLAST of Carg07931-RA vs. ExPASy TrEMBL
Match: A0A1S3B1P6 (probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 6.7e-154
Identity = 269/287 (93.73%), Postives = 280/287 (97.56%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNS-GAPKAHDLSSIV 60
           MAKHRQ RFP+RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNS G+ K HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGK 120
           RKTSD+VDEEKGEQW EVISWEPRAF+YHNFLTKEECEYLISLAKPHMQKS+VVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFIYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDK IR IEKRI+DFSF+PVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTIRTIEKRISDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKP 240
           FLDEYNTKNGGQRIAT+LMYLSDVEEGGETVFPAAKGNFSSVPWW+ELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of Carg07931-RA vs. ExPASy TrEMBL
Match: A0A0A0KJH9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507320 PE=4 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.1e-153
Identity = 268/287 (93.38%), Postives = 280/287 (97.56%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNS-GAPKAHDLSSIV 60
           MAKHRQ RFP+RKSSSSSTL+FTLLIMFTFVILILLALGILSIPGNS G+ K HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTLVFTLLIMFTFVILILLALGILSIPGNSGGSTKVHDLSSIV 60

Query: 61  RKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGK 120
           RKTSD+VDEEKGEQW EVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKS+VVDSETG+
Sbjct: 61  RKTSDDVDEEKGEQWVEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSTVVDSETGQ 120

Query: 121 SKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRGRDK +R IEKR++DFSF+PVEHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGRDKTVRTIEKRLSDFSFIPVEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKP 240
           FLDEYNTKNGGQRIAT+LMYLSDVEEGGETVFPAAKGNFSSVPWW+ELSDCGKKGLSVKP
Sbjct: 181 FLDEYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287

[Close]

BLAST of Carg07931-RA vs. ExPASy TrEMBL
Match: A0A6J1IRF1 (probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=4 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 2.8e-152
Identity = 266/287 (92.68%), Postives = 280/287 (97.56%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNS-GAPKAHDLSSIV 60
           MAKHRQ RFP+RKSSSSST+IFTLLIMFTFVILILLALGILSIPGNS G+PK HDLSSIV
Sbjct: 1   MAKHRQSRFPTRKSSSSSTIIFTLLIMFTFVILILLALGILSIPGNSGGSPKVHDLSSIV 60

Query: 61  RKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGK 120
           RKTS++VDEEKGE+WAEVISWEPRAFVYHNFLTKEECEYLIS AKPHMQKS+VVDSETGK
Sbjct: 61  RKTSEDVDEEKGERWAEVISWEPRAFVYHNFLTKEECEYLISHAKPHMQKSTVVDSETGK 120

Query: 121 SKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDY 180
           SKDSRVRTSSGTFLPRG DKII  IEKRIADF+F+P+EHGEGLQVLHYEVGQKYEPHFDY
Sbjct: 121 SKDSRVRTSSGTFLPRGGDKIISTIEKRIADFTFIPIEHGEGLQVLHYEVGQKYEPHFDY 180

Query: 181 FLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKP 240
           FLD+YNTKNGGQRIAT+LMYLSDVEEGGETVFPAAKGNFSSVPWW+ELSDCGKKGLSVKP
Sbjct: 181 FLDDYNTKNGGQRIATVLMYLSDVEEGGETVFPAAKGNFSSVPWWNELSDCGKKGLSVKP 240

Query: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYKA 287
           KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRV+EYKA
Sbjct: 241 KRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVDEYKA 287

[Close]

BLAST of Carg07931-RA vs. TAIR 10
Match: AT5G66060.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 459.5 bits (1181), Expect = 1.9e-129
Identity = 223/288 (77.43%), Postives = 254/288 (88.19%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIP-GNSGAPKAHDLSSIV 60
           MA+ R  R PS + SS STL+F +LIM TFVILILLA GILS+P  N+G+ KA+DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKTSDE--VDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSET 120
           RKT      D+ K E+W E+ISWEPRA VYHNFLTKEEC+YLI LAKPHM+KS+VVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFLTKEECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RGRDK IR IEKRI+DF+F+PVEHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSV 240
           DYF+DEYNT+NGGQRIAT+LMYLSDVEEGGETVFPAAKGN+S+VPWW+ELS+CGK GLSV
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGGLSV 241

Query: 241 KPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286
           KPK GDALLFWSM PDA+LDPSSLHGGC VIKGNKWS+TKW+RV EYK
Sbjct: 242 KPKMGDALLFWSMTPDATLDPSSLHGGCAVIKGNKWSSTKWLRVHEYK 288

[Close]

BLAST of Carg07931-RA vs. TAIR 10
Match: AT1G20270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 431.8 bits (1109), Expect = 4.3e-121
Identity = 208/287 (72.47%), Postives = 245/287 (85.37%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPGNSGAPKAHDLSSIVR 60
           MAK R  RF +RK S+   ++F +L M T V+L+LLA G+ S+P N+      DLS   R
Sbjct: 1   MAKLRHSRFQARKWSTLMLVLF-MLFMLTIVLLMLLAFGVFSLPINNDESSPIDLSYFRR 60

Query: 61  KTSDEVD--EEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETG 120
             ++  +   ++G+QW EV+SWEPRAFVYHNFL+KEECEYLISLAKPHM KS+VVDSETG
Sbjct: 61  AATERSEGLGKRGDQWTEVLSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETG 120

Query: 121 KSKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFD 180
           KSKDSRVRTSSGTFL RGRDKII+ IEKRIAD++F+P +HGEGLQVLHYE GQKYEPH+D
Sbjct: 121 KSKDSRVRTSSGTFLRRGRDKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYD 180

Query: 181 YFLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVK 240
           YF+DE+NTKNGGQR+ATMLMYLSDVEEGGETVFPAA  NFSSVPW++ELS+CGKKGLSVK
Sbjct: 181 YFVDEFNTKNGGQRMATMLMYLSDVEEGGETVFPAANMNFSSVPWYNELSECGKKGLSVK 240

Query: 241 PKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286
           P+ GDALLFWSM+PDA+LDP+SLHGGCPVI+GNKWS+TKWM V EYK
Sbjct: 241 PRMGDALLFWSMRPDATLDPTSLHGGCPVIRGNKWSSTKWMHVGEYK 286

[Close]

BLAST of Carg07931-RA vs. TAIR 10
Match: AT2G17720.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 421.4 bits (1082), Expect = 5.8e-118
Identity = 204/281 (72.60%), Postives = 240/281 (85.41%), Query Frame = 0


Query: 8   RFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIP-GNSGAPKAHDLSSIVRK--TSD 67
           R+  RKS S ST  FT+LI+   VILILL LGILS+P  N  + K +DL++IVRK  TS 
Sbjct: 10  RYQPRKSVSRSTQAFTVLILLLVVILILLGLGILSLPNANRNSSKTNDLTNIVRKSETSS 69

Query: 68  EVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSETGKSKDSR 127
             +E  GE+W EVISWEPRA VYHNFLT EECE+LISLAKP M KS+VVD +TG SKDSR
Sbjct: 70  GDEEGNGERWVEVISWEPRAVVYHNFLTNEECEHLISLAKPSMVKSTVVDEKTGGSKDSR 129

Query: 128 VRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHFDYFLDEY 187
           VRTSSGTFL RG D+++  IEKRI+DF+F+PVE+GEGLQVLHY+VGQKYEPH+DYFLDE+
Sbjct: 130 VRTSSGTFLRRGHDEVVEVIEKRISDFTFIPVENGEGLQVLHYQVGQKYEPHYDYFLDEF 189

Query: 188 NTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLSVKPKRGDA 247
           NTKNGGQRIAT+LMYLSDV++GGETVFPAA+GN S+VPWW+ELS CGK+GLSV PK+ DA
Sbjct: 190 NTKNGGQRIATVLMYLSDVDDGGETVFPAARGNISAVPWWNELSKCGKEGLSVLPKKRDA 249

Query: 248 LLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEYK 286
           LLFW+M+PDASLDPSSLHGGCPV+KGNKWS+TKW  V E+K
Sbjct: 250 LLFWNMRPDASLDPSSLHGGCPVVKGNKWSSTKWFHVHEFK 290

[Close]

BLAST of Carg07931-RA vs. TAIR 10
Match: AT4G35810.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 401.0 bits (1029), Expect = 8.1e-112
Identity = 198/288 (68.75%), Postives = 232/288 (80.56%), Query Frame = 0


Query: 3   KHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIPG-NSGAPKAHDLSSIV-- 62
           K +Q R   RKS S+ T  FT++++  FVILIL+ LGI S+P  N  +    DL++IV  
Sbjct: 4   KPKQLRNKPRKSFSTQT--FTVVVLVLFVILILVGLGIFSLPSTNKTSSMPMDLTTIVQT 63

Query: 63  ---RKTSDEVDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSE 122
              R++  + ++  G++W EVISWEPRAFVYHNFLT EECE+LISLAKP M KS VVD +
Sbjct: 64  IQERESFGDEEDGNGDRWLEVISWEPRAFVYHNFLTNEECEHLISLAKPSMMKSKVVDVK 123

Query: 123 TGKSKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPH 182
           TGKS DSRVRTSSGTFL RG D+I+  IE RI+DF+F+P E+GEGLQVLHYEVGQ+YEPH
Sbjct: 124 TGKSIDSRVRTSSGTFLNRGHDEIVEEIENRISDFTFIPPENGEGLQVLHYEVGQRYEPH 183

Query: 183 FDYFLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKGLS 242
            DYF DE+N + GGQRIAT+LMYLSDV+EGGETVFPAAKGN S VPWWDELS CGK+GLS
Sbjct: 184 HDYFFDEFNVRKGGQRIATVLMYLSDVDEGGETVFPAAKGNVSDVPWWDELSQCGKEGLS 243

Query: 243 VKPKRGDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSATKWMRVEEY 285
           V PK+ DALLFWSMKPDASLDPSSLHGGCPVIKGNKWS+TKW  V EY
Sbjct: 244 VLPKKRDALLFWSMKPDASLDPSSLHGGCPVIKGNKWSSTKWFHVHEY 289

[Close]

BLAST of Carg07931-RA vs. TAIR 10
Match: AT5G66060.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 352.8 bits (904), Expect = 2.5e-97
Identity = 177/237 (74.68%), Postives = 205/237 (86.50%), Query Frame = 0


Query: 1   MAKHRQPRFPSRKSSSSSTLIFTLLIMFTFVILILLALGILSIP-GNSGAPKAHDLSSIV 60
           MA+ R  R PS + SS STL+F +LIM TFVILILLA GILS+P  N+G+ KA+DL+SIV
Sbjct: 2   MARPRNHR-PSARKSSHSTLVFAVLIMSTFVILILLAFGILSVPSNNAGSSKANDLTSIV 61

Query: 61  RKTSDE--VDEEKGEQWAEVISWEPRAFVYHNFLTKEECEYLISLAKPHMQKSSVVDSET 120
           RKT      D+ K E+W E+ISWEPRA VYHNFL  EEC+YLI LAKPHM+KS+VVD +T
Sbjct: 62  RKTLQRSGEDDSKNERWVEIISWEPRASVYHNFL--EECKYLIELAKPHMEKSTVVDEKT 121

Query: 121 GKSKDSRVRTSSGTFLPRGRDKIIRNIEKRIADFSFVPVEHGEGLQVLHYEVGQKYEPHF 180
           GKS DSRVRTSSGTFL RGRDK IR IEKRI+DF+F+PVEHGEGLQVLHYE+GQKYEPH+
Sbjct: 122 GKSTDSRVRTSSGTFLARGRDKTIREIEKRISDFTFIPVEHGEGLQVLHYEIGQKYEPHY 181

Query: 181 DYFLDEYNTKNGGQRIATMLMYLSDVEEGGETVFPAAKGNFSSVPWWDELSDCGKKG 235
           DYF+DEYNT+NGGQRIAT+LMYLSDVEEGGETVFPAAKGN+S+VPWW+ELS+CGK G
Sbjct: 182 DYFMDEYNTRNGGQRIATVLMYLSDVEEGGETVFPAAKGNYSAVPWWNELSECGKGG 235

The following BLAST results are available for this feature:

BLAST of Carg07931-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022926539.1	1.2e-162	100.00	probable prolyl 4-hydroxylase 10 [Cucurbita moschata] >XP_023517633.1 probable p...	[more]
XP_023003922.1	5.2e-161	99.30	probable prolyl 4-hydroxylase 10 [Cucurbita maxima]	[more]
XP_008440878.1	1.4e-153	93.73	PREDICTED: probable prolyl 4-hydroxylase 10 [Cucumis melo]	[more]
XP_004134841.1	2.3e-153	93.38	probable prolyl 4-hydroxylase 10 [Cucumis sativus] >XP_031742781.1 probable prol...	[more]
XP_038881910.1	6.8e-153	93.71	probable prolyl 4-hydroxylase 10 isoform X2 [Benincasa hispida]	[more]

BLAST of Carg07931-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JZ24	2.7e-128	77.43	Probable prolyl 4-hydroxylase 10 OS=Arabidopsis thaliana OX=3702 GN=P4H10 PE=3 S...	[more]
Q9LN20	6.1e-120	72.47	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]
Q24JN5	8.2e-117	72.60	Prolyl 4-hydroxylase 5 OS=Arabidopsis thaliana OX=3702 GN=P4H5 PE=2 SV=1	[more]
F4JNU8	1.1e-110	68.75	Probable prolyl 4-hydroxylase 8 OS=Arabidopsis thaliana OX=3702 GN=P4H8 PE=3 SV=...	[more]
F4JAU3	1.9e-65	56.94	Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1	[more]

BLAST of Carg07931-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EFG6	6.0e-163	100.00	probable prolyl 4-hydroxylase 10 OS=Cucurbita moschata OX=3662 GN=LOC111433654 P...	[more]
A0A6J1KNY9	2.5e-161	99.30	probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111497367 PE=...	[more]
A0A1S3B1P6	6.7e-154	93.73	probable prolyl 4-hydroxylase 10 OS=Cucumis melo OX=3656 GN=LOC103485167 PE=4 SV...	[more]
A0A0A0KJH9	1.1e-153	93.38	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A6J1IRF1	2.8e-152	92.68	probable prolyl 4-hydroxylase 10 OS=Cucurbita maxima OX=3661 GN=LOC111478689 PE=...	[more]

BLAST of Carg07931-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66060.1	1.9e-129	77.43	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G20270.1	4.3e-121	72.47	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G17720.1	5.8e-118	72.60	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G35810.1	8.1e-112	68.75	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G66060.2	2.5e-97	74.68	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 82..280 e-value: 1.7E-65 score: 233.6
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 75..281 e-value: 7.3E-82 score: 276.1
None	No IPR available	PANTHER	PTHR10869:SF157	PROLYL 4-HYDROXYLASE 10-RELATED	coord: 1..285
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 162..280 e-value: 6.3E-22 score: 78.3
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 1..285
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 158..281 score: 12.587094

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg07931	Carg07931	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg07931-RA:1	Carg07931-RA:1	exon
Carg07931-RA:2	Carg07931-RA:2	exon
Carg07931-RA:3	Carg07931-RA:3	exon
Carg07931-RA:4	Carg07931-RA:4	exon
Carg07931-RA:5	Carg07931-RA:5	exon
Carg07931-RA:6	Carg07931-RA:6	exon
Carg07931-RA:7	Carg07931-RA:7	exon
Carg07931-RA:8	Carg07931-RA:8	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg07931-RA:five_prime_utr	Carg07931-RA:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg07931-RA:cds	Carg07931-RA:cds	CDS
Carg07931-RA:cds	Carg07931-RA:cds_2	CDS
Carg07931-RA:cds	Carg07931-RA:cds_3	CDS
Carg07931-RA:cds	Carg07931-RA:cds_4	CDS
Carg07931-RA:cds	Carg07931-RA:cds_5	CDS
Carg07931-RA:cds	Carg07931-RA:cds_6	CDS
Carg07931-RA:cds	Carg07931-RA:cds_7	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg07931-RA:three_prime_utr	Carg07931-RA:three_prime_utr	three_prime_UTR
Carg07931-RA:three_prime_utr	Carg07931-RA:three_prime_utr_2	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg07931-RA	Carg07931-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0004656	procollagen-proline 4-dioxygenase activity