Carg23321 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23321
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionThreonyl-tRNA synthetase
LocationCarg_Chr06: 10503027 .. 10505410 (-)
RNA-Seq ExpressionCarg23321
SyntenyCarg23321
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGCCTATGCTGTTGTTATGCCCTCCAATTGGCCGTAAACCAGTTTCATCCTCTGCTCGCCTCCTCTCTGCAACTTCTCTCCGCCTTTTCTCATCAGCTGTACACCCAACAGCAGCGGAGACACAAGCTTCCTCGTCGCCCGCAACTGTAGCTTCAATGGCGGTGCAGCACCCCAAAGATGACGACTACCTCAGTTCCGTCATTCCTAAGCGCGTTCAGCTTTTCCAGTCGATACAGGCAAAGCTACTTGCTGACCGGTGTTCTCTTCCCTCTGATCCAATCGAGTTAGTATGACGTTAGAAACTATGGAAATGGATAGCCTATGCTTGTATTATTTGAATTCCGAATTTTACTTGGTTCTTTGCTTTTTAGGATATCCTTACCGGATGGGACGGTGAAGGAGGGTAAGAAATGGCAAACAGCGCCAATAGATGTTGCTAGAGAAATCTCGAAAAGTTTGGCTGTGAACGCTTTGATTGCGCAAGTTGATGGAGTTCTATGGGACATGAGCAGGCCGCTTGAAGATGATTGTGAGCTTAAGCTGTTCACCTTCGAAAGTGATGAAGGGCGCGACACTTTTTGGCATTCTAGTGCTCATATACTTGGACAGGTTGATAGCCCCATCTATTTTCATACACTCAATCTGAACTCATTAATTTCAATCCTAACACCGTTTCAACTGTGTTGTTTTTCCGGGTTTAGTCGCTTGAGATGGAGTATGGTTGCAAGCTTTGCATTGGACCCTGTACCACTAGAGGAGAGGTATGTGTGTGTGTGTATAGTATTAAAAGAAATGGGGGAGAAGATATTCTGTAAGTGAAAATCATCTATTATTAAGCGTGACCAAAATTTCCTTAGTAATTGCTATTTTGGTTGTCCAACGGAAAAAGTGCCAAGGTCCTTCACCTTCGATGAAGGATCCAACATCTTTCGGAGGAAAAGATCTAGAATTATTTTGTGATTGTATACTCGATCATCTTTTCTGTGGTGTAGATGATCCCTCCTTTCTAGGTAGGGGTGATCATTGATTTAGTTCCTTGGTTTTTCTTTTTTTGGCTAAATTGTGAATTGAATTGGTGACTTTTATCTTTTGGTGTATGAAATTGAACAAATGTCCAAATAAGCAAAAATAGAAAAAAGTCGAACTGGTCGGTTCGGTTTTTTAAGAAGGAAGAATGCTTTTGAATACAGTCTACAGTGTTGACTTCTCCCACATAAGCCTTTCCAGGTAGTGATCGACTTGCTGAAATAAGTTACATTATCGTAACTTGAAAGGGGATAGCTTTGGCAGTCGGGATAAACTTTTTGTTGCATTGTTCAGTTGACTTATATAGGGGTGTTAAGAGTTTCAACACCGTATGTAACTTCCCTCTATTTTTATTCTTGTAGTTTGGTTTACTCAGATTAATGGACTTTCATGACAGGGATTCTATTACGATGCTTTTTATGATGATTTATACTTGAATGATGATCACTTCAAGCAAATTGAGTCTGGTGCACTGAAGGCTGTATCGGTATTGCTCTTTCTCTTTTCTCTTGACGAGTTTACACCATTTATTTCATATAATTAAAATCTAGTTATATATATATATATATATATATATATATATATATATATATATGTATATATATATATATTACCATGTATTTACACGTGGAAGAAGGATGTTTATAACTATATAAAGAATGTCCACCACTATTTCTTTCTTTCTTTCTTTCTTTATTAGTTTTTGTTGGGAGGGTGAGGACTGGTGTTGTTTGTAATCTCAGTCTTTCACATTTGTAAAGGCCTAACAACTAAGGTTTTGGGACTAATTTTGTATGTGGAAATGCTATGCTAGAGAGTCCTCACCCTTACCCTAGTTAACTTTAGCTTCATTTGTTATAAAGAATGTAAAGATTCAGTGCTAGTAAACTAACCTTCTGGTTGTTCCAATTTTTCGGAACTAGTAGTGAATCAATAATTTGACAATTGATATCGAAATGTATAATAAGCTAGAATCATGTTGCACATATCTGTTGCTTCCTAAACTTTAGCATTGCTTGCTTATTATCTTTTTATTCACTGACTGTATTAGAGACTTTTATTTTGGCTCGAAATGAAGTACTGGAATATTTTTCCCAATAAAGCATTCAATAATCTGGTTTCATGCATATTTCATTTTTCTCAATGGATGGTTATTCATAAAAGAAAAAAAAAGATCTATGTTTCATGCATGGAACATCATCATTACAGATGATACTTCTTGTTATCAAAGGAATAATTTGATCTATTTCTTTTAAATTTTCAGGAGAGACAACCTTTTGAGCGCATTGAAGTTTCAAGGCAACAAGCTCTTGAAATGTTTTCTGACAATAAATTCAAGGCAATCGGATATCTTTAA

mRNA sequence

CTGCCTATGCTGTTGTTATGCCCTCCAATTGGCCGTAAACCAGTTTCATCCTCTGCTCGCCTCCTCTCTGCAACTTCTCTCCGCCTTTTCTCATCAGCTGTACACCCAACAGCAGCGGAGACACAAGCTTCCTCGTCGCCCGCAACTGTAGCTTCAATGGCGGTGCAGCACCCCAAAGATGACGACTACCTCAGTTCCGTCATTCCTAAGCGCGTTCAGCTTTTCCAGTCGATACAGGCAAAGCTACTTGCTGACCGGTGTTCTCTTCCCTCTGATCCAATCGAGATATCCTTACCGGATGGGACGGTGAAGGAGGGTAAGAAATGGCAAACAGCGCCAATAGATGTTGCTAGAGAAATCTCGAAAAGTTTGGCTGTGAACGCTTTGATTGCGCAAGTTGATGGAGTTCTATGGGACATGAGCAGGCCGCTTGAAGATGATTGTGAGCTTAAGCTGTTCACCTTCGAAAGTGATGAAGGGCGCGACACTTTTTGGCATTCTAGTGCTCATATACTTGGACAGTCGCTTGAGATGGAGTATGGTTGCAAGCTTTGCATTGGACCCTGTACCACTAGAGGAGAGGGATTCTATTACGATGCTTTTTATGATGATTTATACTTGAATGATGATCACTTCAAGCAAATTGAGTCTGGTGCACTGAAGGCTGTATCGGAGAGACAACCTTTTGAGCGCATTGAAGTTTCAAGGCAACAAGCTCTTGAAATGTTTTCTGACAATAAATTCAAGGCAATCGGATATCTTTAA

Coding sequence (CDS)

ATGCTGTTGTTATGCCCTCCAATTGGCCGTAAACCAGTTTCATCCTCTGCTCGCCTCCTCTCTGCAACTTCTCTCCGCCTTTTCTCATCAGCTGTACACCCAACAGCAGCGGAGACACAAGCTTCCTCGTCGCCCGCAACTGTAGCTTCAATGGCGGTGCAGCACCCCAAAGATGACGACTACCTCAGTTCCGTCATTCCTAAGCGCGTTCAGCTTTTCCAGTCGATACAGGCAAAGCTACTTGCTGACCGGTGTTCTCTTCCCTCTGATCCAATCGAGATATCCTTACCGGATGGGACGGTGAAGGAGGGTAAGAAATGGCAAACAGCGCCAATAGATGTTGCTAGAGAAATCTCGAAAAGTTTGGCTGTGAACGCTTTGATTGCGCAAGTTGATGGAGTTCTATGGGACATGAGCAGGCCGCTTGAAGATGATTGTGAGCTTAAGCTGTTCACCTTCGAAAGTGATGAAGGGCGCGACACTTTTTGGCATTCTAGTGCTCATATACTTGGACAGTCGCTTGAGATGGAGTATGGTTGCAAGCTTTGCATTGGACCCTGTACCACTAGAGGAGAGGGATTCTATTACGATGCTTTTTATGATGATTTATACTTGAATGATGATCACTTCAAGCAAATTGAGTCTGGTGCACTGAAGGCTGTATCGGAGAGACAACCTTTTGAGCGCATTGAAGTTTCAAGGCAACAAGCTCTTGAAATGTTTTCTGACAATAAATTCAAGGCAATCGGATATCTTTAA

Protein sequence

MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDDYLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISKSLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFKAIGYL
Homology
BLAST of Carg23321 vs. NCBI nr
Match: KAG7029174.1 (Threonine--tRNA ligase, mitochondrial 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 501.5 bits (1290), Expect = 4.2e-138
Identity = 252/252 (100.00%), Postives = 252/252 (100.00%), Query Frame = 0

Query: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDD 60
           MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDD
Sbjct: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDD 60

Query: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120
           YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK
Sbjct: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120

Query: 121 SLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180
           SLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC
Sbjct: 121 SLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180

Query: 181 KLCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM 240
           KLCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM
Sbjct: 181 KLCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM 240

Query: 241 FSDNKFKAIGYL 253
           FSDNKFKAIGYL
Sbjct: 241 FSDNKFKAIGYL 252

BLAST of Carg23321 vs. NCBI nr
Match: XP_022932568.1 (threonine--tRNA ligase, mitochondrial 1-like [Cucurbita moschata])

HSP 1 Score: 485.3 bits (1248), Expect = 3.1e-133
Identity = 244/247 (98.79%), Postives = 245/247 (99.19%), Query Frame = 0

Query: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDD 60
           MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPAT+ASMAVQHPKDDD
Sbjct: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATLASMAVQHPKDDD 60

Query: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120
           YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK
Sbjct: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120

Query: 121 SLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180
           SLA NALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC
Sbjct: 121 SLAANALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180

Query: 181 KLCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM 240
           KLCIGPCTTRGEGFYYDAFYDDL LNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM
Sbjct: 181 KLCIGPCTTRGEGFYYDAFYDDLCLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM 240

Query: 241 FSDNKFK 248
           FSDNKFK
Sbjct: 241 FSDNKFK 247

BLAST of Carg23321 vs. NCBI nr
Match: XP_023540968.1 (threonine--tRNA ligase, mitochondrial 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 485.3 bits (1248), Expect = 3.1e-133
Identity = 244/247 (98.79%), Postives = 245/247 (99.19%), Query Frame = 0

Query: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDD 60
           MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPAT+ASMAVQHPKDDD
Sbjct: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATLASMAVQHPKDDD 60

Query: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120
           YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK
Sbjct: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120

Query: 121 SLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180
           SLA NALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC
Sbjct: 121 SLAANALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180

Query: 181 KLCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM 240
           KLCIGPCTTRGEGFYYDAFYDDL LNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM
Sbjct: 181 KLCIGPCTTRGEGFYYDAFYDDLCLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM 240

Query: 241 FSDNKFK 248
           FSDNKFK
Sbjct: 241 FSDNKFK 247

BLAST of Carg23321 vs. NCBI nr
Match: XP_022972266.1 (threonine--tRNA ligase, mitochondrial 1-like [Cucurbita maxima] >XP_022972267.1 threonine--tRNA ligase, mitochondrial 1-like [Cucurbita maxima])

HSP 1 Score: 474.2 bits (1219), Expect = 7.2e-130
Identity = 240/247 (97.17%), Postives = 242/247 (97.98%), Query Frame = 0

Query: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDD 60
           MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHP +AETQASSSPA +ASMAVQHPKDDD
Sbjct: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHP-SAETQASSSPAILASMAVQHPKDDD 60

Query: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120
           YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK
Sbjct: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120

Query: 121 SLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180
           SLA NALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC
Sbjct: 121 SLAANALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180

Query: 181 KLCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM 240
           KLCIGPCTTRGEGFYYDAFYDDL LNDDHFKQIESGALKA SERQPFERIEVSRQQALEM
Sbjct: 181 KLCIGPCTTRGEGFYYDAFYDDLCLNDDHFKQIESGALKAASERQPFERIEVSRQQALEM 240

Query: 241 FSDNKFK 248
           FSDNKFK
Sbjct: 241 FSDNKFK 246

BLAST of Carg23321 vs. NCBI nr
Match: KAG6597727.1 (Threonine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 465.3 bits (1196), Expect = 3.3e-127
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0

Query: 14  SSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDDYLSSVIPKRVQLF 73
           SSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDDYLSSVIPKRVQLF
Sbjct: 45  SSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDDYLSSVIPKRVQLF 104

Query: 74  QSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISKSLAVNALIAQVDG 133
           QSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISKSLAVNALIAQVDG
Sbjct: 105 QSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISKSLAVNALIAQVDG 164

Query: 134 VLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEG 193
           VLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEG
Sbjct: 165 VLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEG 224

Query: 194 FYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 248
           FYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK
Sbjct: 225 FYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 278

BLAST of Carg23321 vs. ExPASy Swiss-Prot
Match: O04630 (Threonine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=THRRS PE=1 SV=3)

HSP 1 Score: 302.4 bits (773), Expect = 4.9e-81
Identity = 146/228 (64.04%), Postives = 184/228 (80.70%), Query Frame = 0

Query: 20  LSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDDYLSSVIPKRVQLFQSIQAK 79
           L+A S+R F+++   ++    +SSS  TV +MA  HPKD+ YLS+VIPKR++LF+ IQA 
Sbjct: 5   LTARSIRRFTTS--SSSLPLLSSSSFCTVPTMAANHPKDEAYLSAVIPKRIKLFEQIQAN 64

Query: 80  LLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISKSLAVNALIAQVDGVLWDMS 139
            L +  SLP DPI+++LPDG VKEGKKW+T P+D+A +ISK LA +ALI+ VD VLWDM+
Sbjct: 65  QLENLKSLPHDPIKVTLPDGNVKEGKKWETTPMDIAAQISKGLANSALISAVDDVLWDMN 124

Query: 140 RPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAF 199
           RPLE DC+L+LF F+SD+GRDT WHSSAHILGQ+LE EYGC+LCIGPCTTRGEGFYYD F
Sbjct: 125 RPLEGDCKLELFKFDSDKGRDTLWHSSAHILGQALEQEYGCQLCIGPCTTRGEGFYYDGF 184

Query: 200 YDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 248
           Y +L L+D+HF  IE+GA KA  E QPFERIEV++ QALEMFS+N FK
Sbjct: 185 YGELGLSDNHFPSIEAGAAKAAKEAQPFERIEVTKDQALEMFSENNFK 230

BLAST of Carg23321 vs. ExPASy Swiss-Prot
Match: Q8GZ45 (Probable threonine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g17960 PE=2 SV=2)

HSP 1 Score: 225.7 bits (574), Expect = 5.8e-58
Identity = 104/163 (63.80%), Postives = 134/163 (82.21%), Query Frame = 0

Query: 55  HPKDDDYLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEIS-LPDGTVKEGKKWQTAPID 114
           HPKD+ YLSSVIPKR++LF+ IQA+ L    S P DPI+I+ LPDG  KEG++W+T+P+D
Sbjct: 6   HPKDEAYLSSVIPKRIRLFEEIQAEQLEQLQSRPHDPIKITLLPDGIEKEGRRWETSPMD 65

Query: 115 VAREISKSLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQS 174
           +A +ISK LA +AL++ V+ VLWDM+RPLE DC L++F F+SD+GR+TFWHSSAHILGQ+
Sbjct: 66  IAVQISKGLAKSALVSSVNHVLWDMNRPLEGDCSLEIFGFDSDQGRNTFWHSSAHILGQA 125

Query: 175 LEMEYGCKLCIGPCTTRGEGFYYDA-FYDDLYLNDDHFKQIES 216
           LE EYGCKLCIGPC  R EGFYYD+ +Y +L LND+HF  IE+
Sbjct: 126 LEQEYGCKLCIGPCEPRDEGFYYDSLYYGELGLNDNHFPNIEA 168

BLAST of Carg23321 vs. ExPASy Swiss-Prot
Match: Q54J66 (Probable threonine--tRNA ligase 1, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=thrS1 PE=3 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.5e-42
Identity = 87/183 (47.54%), Postives = 126/183 (68.85%), Query Frame = 0

Query: 66  IPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISKSLAVN 125
           + +R+++++S++AK L     +  +PI ++LPDG V  G K +T P D+A+ IS+ LA +
Sbjct: 50  VKERIEIWESLKAKHLESLKDMKEEPINVTLPDGKVVAGIKNKTTPYDIAKGISRGLADS 109

Query: 126 ALIAQVDG-VLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCKLCI 185
            + ++V+G  +WD+SRPLE DC L+L  F+S+EG+ TFWHSSAHILGQ++E  YG +LCI
Sbjct: 110 IVSSKVNGEQIWDISRPLEADCNLQLCKFDSEEGKKTFWHSSAHILGQAMERIYGGQLCI 169

Query: 186 GPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDN 245
           GP T+  EGFYYD    D  ++ + +K I   A K V E+QPFER+ V R  AL MF  N
Sbjct: 170 GPATS--EGFYYDMAMGDKIVSAEDYKLINEVAQKIVQEKQPFERLAVPRDIALTMFKFN 229

Query: 246 KFK 248
           K+K
Sbjct: 230 KYK 230

BLAST of Carg23321 vs. ExPASy Swiss-Prot
Match: P52709 (Threonine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=tars-1 PE=3 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 4.9e-41
Identity = 80/192 (41.67%), Postives = 126/192 (65.62%), Query Frame = 0

Query: 56  PKDDDYLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVA 115
           P D     + I +R++L+  ++A+  A+  +  S+PI+I+LPDG + EGK W+T P ++A
Sbjct: 46  PTDMAPWPAFIEERIKLWDKLKAEYDAEIAAKESEPIQITLPDGKIHEGKTWRTTPFEIA 105

Query: 116 REISKSLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLE 175
             ISK LA  A+IA+V+G +WD+ RP E + +L+L  F+ DE +  FWHSSAH+LG+++E
Sbjct: 106 ERISKGLAEAAVIAKVNGAVWDLDRPFEGNAKLELLKFDDDEAKQVFWHSSAHVLGEAME 165

Query: 176 MEYGCKLCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQ 235
              G  LC GP     EGFYYD ++++  +  D F +I+     AV ++Q FER+E++++
Sbjct: 166 RYCGGHLCYGPPIQ--EGFYYDMWHENRTICPDDFPKIDQIVKAAVKDKQKFERLEMTKE 225

Query: 236 QALEMFSDNKFK 248
             LEMF  N+FK
Sbjct: 226 DLLEMFKYNEFK 235

BLAST of Carg23321 vs. ExPASy Swiss-Prot
Match: P87144 (Threonine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ths1 PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.5e-40
Identity = 86/192 (44.79%), Postives = 120/192 (62.50%), Query Frame = 0

Query: 60  DYLSSVIP---KRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAR 119
           D LS  IP    R+ LF  +Q +      + P + I+I+LPDG V +G  W+T PI +A 
Sbjct: 14  DNLSLEIPFIQHRLDLFDKLQKEYKESLATKPREEIDITLPDGKVIKGTSWETTPISIAA 73

Query: 120 EISKSLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEM 179
            ISK LA    +A V+G  WD++RPLE  C LKL  F   EG+  FWHSSAHILG++ E+
Sbjct: 74  SISKGLADRVTVAIVNGEPWDLTRPLEASCTLKLCDFNDPEGKRVFWHSSAHILGEATEL 133

Query: 180 EYGCKLCIGPCTTRGEGFYYDAFYDD-LYLNDDHFKQIESGALKAVSERQPFERIEVSRQ 239
            + C LCIGP T   EGF+Y+   D+   + +D +  IES A +A+ ++QPFER+ +S++
Sbjct: 134 SFHCHLCIGPPT--DEGFFYEMGIDNGRVITNDDYSSIESYAKQAIKQKQPFERLVISKE 193

Query: 240 QALEMFSDNKFK 248
             LEMF  NK+K
Sbjct: 194 GLLEMFRYNKYK 203

BLAST of Carg23321 vs. ExPASy TrEMBL
Match: A0A6J1F237 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111439084 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.5e-133
Identity = 244/247 (98.79%), Postives = 245/247 (99.19%), Query Frame = 0

Query: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDD 60
           MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPAT+ASMAVQHPKDDD
Sbjct: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATLASMAVQHPKDDD 60

Query: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120
           YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK
Sbjct: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120

Query: 121 SLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180
           SLA NALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC
Sbjct: 121 SLAANALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180

Query: 181 KLCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM 240
           KLCIGPCTTRGEGFYYDAFYDDL LNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM
Sbjct: 181 KLCIGPCTTRGEGFYYDAFYDDLCLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM 240

Query: 241 FSDNKFK 248
           FSDNKFK
Sbjct: 241 FSDNKFK 247

BLAST of Carg23321 vs. ExPASy TrEMBL
Match: A0A6J1I4C8 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111470850 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 3.5e-130
Identity = 240/247 (97.17%), Postives = 242/247 (97.98%), Query Frame = 0

Query: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDD 60
           MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHP +AETQASSSPA +ASMAVQHPKDDD
Sbjct: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHP-SAETQASSSPAILASMAVQHPKDDD 60

Query: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120
           YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK
Sbjct: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120

Query: 121 SLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180
           SLA NALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC
Sbjct: 121 SLAANALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180

Query: 181 KLCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM 240
           KLCIGPCTTRGEGFYYDAFYDDL LNDDHFKQIESGALKA SERQPFERIEVSRQQALEM
Sbjct: 181 KLCIGPCTTRGEGFYYDAFYDDLCLNDDHFKQIESGALKAASERQPFERIEVSRQQALEM 240

Query: 241 FSDNKFK 248
           FSDNKFK
Sbjct: 241 FSDNKFK 246

BLAST of Carg23321 vs. ExPASy TrEMBL
Match: A0A6J1F8B6 (Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111441782 PE=3 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.0e-109
Identity = 207/246 (84.15%), Postives = 215/246 (87.40%), Query Frame = 0

Query: 2   LLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDDY 61
           L LC  IGRK VSSS  LLS TSLRL SS   PTA          T ASMA QHPKD+ Y
Sbjct: 4   LCLCSRIGRKAVSSSTSLLSTTSLRLCSSVAQPTA---------GTQASMAAQHPKDEHY 63

Query: 62  LSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISKS 121
           L+SVIPKR+QLFQSIQAKLLADR SLPSDPIEI+LPDGTVK GKKWQTAPIDVAREISKS
Sbjct: 64  LNSVIPKRIQLFQSIQAKLLADRRSLPSDPIEITLPDGTVKAGKKWQTAPIDVAREISKS 123

Query: 122 LAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCK 181
           LA NALIAQVDG LWDM RPLE DCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCK
Sbjct: 124 LAANALIAQVDGALWDMCRPLEGDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCK 183

Query: 182 LCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMF 241
           LCIGPCTTRGEGFYYDAFYDDL LNDDHFK+IESGALKAVSE+QPFERIEVSR+QALE+F
Sbjct: 184 LCIGPCTTRGEGFYYDAFYDDLGLNDDHFKKIESGALKAVSEKQPFERIEVSREQALEIF 240

Query: 242 SDNKFK 248
           SDNKFK
Sbjct: 244 SDNKFK 240

BLAST of Carg23321 vs. ExPASy TrEMBL
Match: A0A6J1J5S0 (Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111481536 PE=3 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.3e-108
Identity = 207/246 (84.15%), Postives = 215/246 (87.40%), Query Frame = 0

Query: 2   LLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDDY 61
           L LC  IGRK VSSS  LLS TSLRL SS    TA          T ASMA QHPKD+ Y
Sbjct: 4   LCLCSRIGRKAVSSSTPLLSTTSLRLCSSVAQLTA---------GTQASMAAQHPKDEHY 63

Query: 62  LSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISKS 121
           L+SVIPKR+QLFQSIQAKLLADR SLPSDPIEI+LPDGTVK GKKWQTAPIDVAREISKS
Sbjct: 64  LNSVIPKRIQLFQSIQAKLLADRRSLPSDPIEITLPDGTVKAGKKWQTAPIDVAREISKS 123

Query: 122 LAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCK 181
           LA NALIAQVDGVLWDM RPLE DCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCK
Sbjct: 124 LAANALIAQVDGVLWDMCRPLEGDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCK 183

Query: 182 LCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMF 241
           LCIGPCTTRGEGFYYDAFYDDL LNDDHFK+IESGALKAVSE+QPFERIEVSR+QALE+F
Sbjct: 184 LCIGPCTTRGEGFYYDAFYDDLGLNDDHFKKIESGALKAVSEKQPFERIEVSREQALEIF 240

Query: 242 SDNKFK 248
           SDNKFK
Sbjct: 244 SDNKFK 240

BLAST of Carg23321 vs. ExPASy TrEMBL
Match: A0A6J1DG60 (Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020133 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.3e-105
Identity = 201/247 (81.38%), Postives = 213/247 (86.23%), Query Frame = 0

Query: 1   MLLLCPPIGRKPVSSSARLLSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDD 60
           MLLL P IGRK  SSS R L  T             AE QAS +PA+VA+M   H KD+ 
Sbjct: 1   MLLLRPRIGRKAASSSVRFLFPT-------------AEIQASPAPASVATMVAHHSKDEA 60

Query: 61  YLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISK 120
           YL++VIPKRVQ+FQSIQA+LLADR SLPSD IEI+LPDGTVKEGKKWQTAPIDVAREISK
Sbjct: 61  YLNTVIPKRVQIFQSIQAQLLADRRSLPSDTIEITLPDGTVKEGKKWQTAPIDVAREISK 120

Query: 121 SLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGC 180
           SLA NALIAQVDGVLWDMSRPLE DCELKLFTFESDEGRDTFWHSSAHILGQ+LEMEYGC
Sbjct: 121 SLAANALIAQVDGVLWDMSRPLEGDCELKLFTFESDEGRDTFWHSSAHILGQALEMEYGC 180

Query: 181 KLCIGPCTTRGEGFYYDAFYDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEM 240
           KLCIGPCTTRGEGFYYD FYDDL LNDDHFKQIESGALKAVSE+QPFERIEV+RQQALEM
Sbjct: 181 KLCIGPCTTRGEGFYYDGFYDDLGLNDDHFKQIESGALKAVSEKQPFERIEVTRQQALEM 234

Query: 241 FSDNKFK 248
           FSDNKFK
Sbjct: 241 FSDNKFK 234

BLAST of Carg23321 vs. TAIR 10
Match: AT5G26830.1 (Threonyl-tRNA synthetase )

HSP 1 Score: 302.4 bits (773), Expect = 3.5e-82
Identity = 146/228 (64.04%), Postives = 184/228 (80.70%), Query Frame = 0

Query: 20  LSATSLRLFSSAVHPTAAETQASSSPATVASMAVQHPKDDDYLSSVIPKRVQLFQSIQAK 79
           L+A S+R F+++   ++    +SSS  TV +MA  HPKD+ YLS+VIPKR++LF+ IQA 
Sbjct: 5   LTARSIRRFTTS--SSSLPLLSSSSFCTVPTMAANHPKDEAYLSAVIPKRIKLFEQIQAN 64

Query: 80  LLADRCSLPSDPIEISLPDGTVKEGKKWQTAPIDVAREISKSLAVNALIAQVDGVLWDMS 139
            L +  SLP DPI+++LPDG VKEGKKW+T P+D+A +ISK LA +ALI+ VD VLWDM+
Sbjct: 65  QLENLKSLPHDPIKVTLPDGNVKEGKKWETTPMDIAAQISKGLANSALISAVDDVLWDMN 124

Query: 140 RPLEDDCELKLFTFESDEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAF 199
           RPLE DC+L+LF F+SD+GRDT WHSSAHILGQ+LE EYGC+LCIGPCTTRGEGFYYD F
Sbjct: 125 RPLEGDCKLELFKFDSDKGRDTLWHSSAHILGQALEQEYGCQLCIGPCTTRGEGFYYDGF 184

Query: 200 YDDLYLNDDHFKQIESGALKAVSERQPFERIEVSRQQALEMFSDNKFK 248
           Y +L L+D+HF  IE+GA KA  E QPFERIEV++ QALEMFS+N FK
Sbjct: 185 YGELGLSDNHFPSIEAGAAKAAKEAQPFERIEVTKDQALEMFSENNFK 230

BLAST of Carg23321 vs. TAIR 10
Match: AT1G17960.1 (Threonyl-tRNA synthetase )

HSP 1 Score: 225.7 bits (574), Expect = 4.1e-59
Identity = 104/163 (63.80%), Postives = 134/163 (82.21%), Query Frame = 0

Query: 55  HPKDDDYLSSVIPKRVQLFQSIQAKLLADRCSLPSDPIEIS-LPDGTVKEGKKWQTAPID 114
           HPKD+ YLSSVIPKR++LF+ IQA+ L    S P DPI+I+ LPDG  KEG++W+T+P+D
Sbjct: 6   HPKDEAYLSSVIPKRIRLFEEIQAEQLEQLQSRPHDPIKITLLPDGIEKEGRRWETSPMD 65

Query: 115 VAREISKSLAVNALIAQVDGVLWDMSRPLEDDCELKLFTFESDEGRDTFWHSSAHILGQS 174
           +A +ISK LA +AL++ V+ VLWDM+RPLE DC L++F F+SD+GR+TFWHSSAHILGQ+
Sbjct: 66  IAVQISKGLAKSALVSSVNHVLWDMNRPLEGDCSLEIFGFDSDQGRNTFWHSSAHILGQA 125

Query: 175 LEMEYGCKLCIGPCTTRGEGFYYDA-FYDDLYLNDDHFKQIES 216
           LE EYGCKLCIGPC  R EGFYYD+ +Y +L LND+HF  IE+
Sbjct: 126 LEQEYGCKLCIGPCEPRDEGFYYDSLYYGELGLNDNHFPNIEA 168

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029174.14.2e-138100.00Threonine--tRNA ligase, mitochondrial 1 [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022932568.13.1e-13398.79threonine--tRNA ligase, mitochondrial 1-like [Cucurbita moschata][more]
XP_023540968.13.1e-13398.79threonine--tRNA ligase, mitochondrial 1-like [Cucurbita pepo subsp. pepo][more]
XP_022972266.17.2e-13097.17threonine--tRNA ligase, mitochondrial 1-like [Cucurbita maxima] >XP_022972267.1 ... [more]
KAG6597727.13.3e-127100.00Threonine--tRNA ligase, mitochondrial 1, partial [Cucurbita argyrosperma subsp. ... [more]
Match NameE-valueIdentityDescription
O046304.9e-8164.04Threonine--tRNA ligase, mitochondrial 1 OS=Arabidopsis thaliana OX=3702 GN=THRRS... [more]
Q8GZ455.8e-5863.80Probable threonine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q54J661.5e-4247.54Probable threonine--tRNA ligase 1, cytoplasmic OS=Dictyostelium discoideum OX=44... [more]
P527094.9e-4141.67Threonine--tRNA ligase, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=tars-1 ... [more]
P871442.5e-4044.79Threonine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / A... [more]
Match NameE-valueIdentityDescription
A0A6J1F2371.5e-13398.79Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111439084 PE=3 SV=1[more]
A0A6J1I4C83.5e-13097.17Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111470850 PE=3 SV=1[more]
A0A6J1F8B62.0e-10984.15Threonyl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111441782 PE=3 SV=1[more]
A0A6J1J5S01.3e-10884.15Threonyl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111481536 PE=3 SV=1[more]
A0A6J1DG601.3e-10581.38Threonyl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111020133 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT5G26830.13.5e-8264.04Threonyl-tRNA synthetase [more]
AT1G17960.14.1e-5963.80Threonyl-tRNA synthetase [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004095TGSPFAMPF02824TGScoord: 92..152
e-value: 1.8E-15
score: 56.7
IPR004095TGSPROSITEPS51880TGScoord: 90..152
score: 19.927185
IPR012675Beta-grasp domain superfamilyGENE3D3.10.20.30coord: 90..151
e-value: 1.7E-25
score: 90.9
NoneNo IPR availableGENE3D3.30.980.10coord: 156..249
e-value: 1.8E-22
score: 81.9
NoneNo IPR availablePANTHERPTHR11451:SF53THREONINE--TRNA LIGASE, CYTOPLASMICcoord: 51..248
NoneNo IPR availableCDDcd01667TGS_ThrRScoord: 92..157
e-value: 1.23125E-25
score: 93.7065
IPR002320Threonine-tRNA ligase, class IIaPANTHERPTHR11451THREONINE-TRNA LIGASEcoord: 51..248
IPR018163Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamilySUPERFAMILY55186ThrRS/AlaRS common domaincoord: 153..247
IPR012676TGS-likeSUPERFAMILY81271TGS-likecoord: 92..153

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23321-RACarg23321-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006435 threonyl-tRNA aminoacylation
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004829 threonine-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding