Carg09258 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09258
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein BIG GRAIN 1-like E
Location	Carg_Chr05: 2288747 .. 2289847 (-)
RNA-Seq Expression	Carg09258
Synteny	Carg09258

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGCTCTCGAGCTCGGAGATGATCAATAAGAAGATGTTCCACCATAGGAGAGCTTCGAAGGAGCTCGAGGTATTCGAAGCTGCTCGATATTTCTCTGATTACAATGAAACTTCGGCGACGATGAATGGTTTCGGCGCAAAGTTTACTCCGAAAGTGAAGAAGAAAGACAAAGGATGGATCAAAGGGAGAATCAGCTTAGACATGCAGGTTTTCGATCTTAACATTGAAGTTCGAGTCGCATTATTGATGTTCGAGAGTAAATTTAACCATGTTTTTTTTTTTCCCGACATAGGTAAAGAACATCCTTAATCTCCCCGAACATTTCCCCCAACATTCTTACAATGTTGAAAAGCATGTTACAAAGGAGAAGAAGTACAAACAACCAAGCTCACCTGGTGGGAGACTAGCAAGCTTTCTAAACTCTCTCTTTAGCCATTCAAGTTTGAAGAAGAAGAAGTCGAAACTTTTTGCGCAATCCGTGGAAGACATAACGGAAGACGAAAGCCGAACGTCGAAGAGAAGGATTAGCATCAGCCATTTTCGATCCTCGAACGCGGCTGCAACGGATGCTAAGTTCATCTATTCTTCGTCTCCGGGCAATAATTCAGGTAAACCCCGAATCAACTTAACCTAAATAAATATATATATATATATATTCTAATCATTTATTCGTATTCTTAACAAATAGGTTTCAGAACACCGCCTCCGCACGTTCAAACCCCGACAAAGAGCTACAAGGAACTATTGTCGTTTTCGAAGTTCACCCGACACGTAAAATCAGCGGAGACGTTGGAGAAACGGGCTTCGAAACAAAAGAAGAAGAATTTGAGTAATTGTTCTTCAGAGAAAGATAGGGTTTGGGTTGAAAAACCATTGGGAGATGAAGAAATGAAGAAAAAATTGAAGAAATTTGAGCATGATATTAATATCATTGATGATATTGATGATGATGATGGTGGAGAAACAGATTCCAGCTCAGATTTGTTTGAATTACAGATCTATGATTTGGATTATTACTCAAATGGGTTGCCTGTTTATGAATCTACTGATATTGATAGCATCAAGAGAAGAAATTCAATCTCTAATGGTGTTTGTTGA

mRNA sequence

ATGTCGCTCTCGAGCTCGGAGATGATCAATAAGAAGATGTTCCACCATAGGAGAGCTTCGAAGGAGCTCGAGGTATTCGAAGCTGCTCGATATTTCTCTGATTACAATGAAACTTCGGCGACGATGAATGGTTTCGGCGCAAAGTTTACTCCGAAAGTGAAGAAGAAAGACAAAGGATGGATCAAAGGGAGAATCAGCTTAGACATGCAGGTAAAGAACATCCTTAATCTCCCCGAACATTTCCCCCAACATTCTTACAATGTTGAAAAGCATGTTACAAAGGAGAAGAAGTACAAACAACCAAGCTCACCTGGTGGGAGACTAGCAAGCTTTCTAAACTCTCTCTTTAGCCATTCAAGTTTGAAGAAGAAGAAGTCGAAACTTTTTGCGCAATCCGTGGAAGACATAACGGAAGACGAAAGCCGAACGTCGAAGAGAAGGATTAGCATCAGCCATTTTCGATCCTCGAACGCGGCTGCAACGGATGCTAAGTTCATCTATTCTTCGTCTCCGGGCAATAATTCAGGTTTCAGAACACCGCCTCCGCACGTTCAAACCCCGACAAAGAGCTACAAGGAACTATTGTCGTTTTCGAAGTTCACCCGACACGTAAAATCAGCGGAGACGTTGGAGAAACGGGCTTCGAAACAAAAGAAGAAGAATTTGAGTAATTGTTCTTCAGAGAAAGATAGGGTTTGGGTTGAAAAACCATTGGGAGATGAAGAAATGAAGAAAAAATTGAAGAAATTTGAGCATGATATTAATATCATTGATGATATTGATGATGATGATGGTGGAGAAACAGATTCCAGCTCAGATTTGTTTGAATTACAGATCTATGATTTGGATTATTACTCAAATGGGTTGCCTGTTTATGAATCTACTGATATTGATAGCATCAAGAGAAGAAATTCAATCTCTAATGGTGTTTGTTGA

Coding sequence (CDS)

ATGTCGCTCTCGAGCTCGGAGATGATCAATAAGAAGATGTTCCACCATAGGAGAGCTTCGAAGGAGCTCGAGGTATTCGAAGCTGCTCGATATTTCTCTGATTACAATGAAACTTCGGCGACGATGAATGGTTTCGGCGCAAAGTTTACTCCGAAAGTGAAGAAGAAAGACAAAGGATGGATCAAAGGGAGAATCAGCTTAGACATGCAGGTAAAGAACATCCTTAATCTCCCCGAACATTTCCCCCAACATTCTTACAATGTTGAAAAGCATGTTACAAAGGAGAAGAAGTACAAACAACCAAGCTCACCTGGTGGGAGACTAGCAAGCTTTCTAAACTCTCTCTTTAGCCATTCAAGTTTGAAGAAGAAGAAGTCGAAACTTTTTGCGCAATCCGTGGAAGACATAACGGAAGACGAAAGCCGAACGTCGAAGAGAAGGATTAGCATCAGCCATTTTCGATCCTCGAACGCGGCTGCAACGGATGCTAAGTTCATCTATTCTTCGTCTCCGGGCAATAATTCAGGTTTCAGAACACCGCCTCCGCACGTTCAAACCCCGACAAAGAGCTACAAGGAACTATTGTCGTTTTCGAAGTTCACCCGACACGTAAAATCAGCGGAGACGTTGGAGAAACGGGCTTCGAAACAAAAGAAGAAGAATTTGAGTAATTGTTCTTCAGAGAAAGATAGGGTTTGGGTTGAAAAACCATTGGGAGATGAAGAAATGAAGAAAAAATTGAAGAAATTTGAGCATGATATTAATATCATTGATGATATTGATGATGATGATGGTGGAGAAACAGATTCCAGCTCAGATTTGTTTGAATTACAGATCTATGATTTGGATTATTACTCAAATGGGTTGCCTGTTTATGAATCTACTGATATTGATAGCATCAAGAGAAGAAATTCAATCTCTAATGGTGTTTGTTGA

Protein sequence

MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGWIKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSSLKKKKSKLFAQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTPPPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGDEEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSIKRRNSISNGVC

Homology

[Close]

BLAST of Carg09258 vs. NCBI nr
Match: KAG6598646.1 (Protein BIG GRAIN 1-like E, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029583.1 Protein BIG GRAIN 1-like E, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 601.7 bits (1550), Expect = 3.7e-168
Identity = 311/311 (100.00%), Postives = 311/311 (100.00%), Query Frame = 0


Query: 1   MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 60
           MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW
Sbjct: 1   MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 60

Query: 61  IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 120
           IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS
Sbjct: 61  IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 120

Query: 121 LKKKKSKLFAQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP 180
           LKKKKSKLFAQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP
Sbjct: 121 LKKKKSKLFAQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP 180

Query: 181 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 240
           PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD
Sbjct: 181 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 240

Query: 241 EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI 300
           EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI
Sbjct: 241 EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI 300

Query: 301 KRRNSISNGVC 312
           KRRNSISNGVC
Sbjct: 301 KRRNSISNGVC 311

[Close]

BLAST of Carg09258 vs. NCBI nr
Match: XP_022997188.1 (protein BIG GRAIN 1-like E [Cucurbita maxima])

HSP 1 Score: 593.6 bits (1529), Expect = 1.0e-165
Identity = 307/311 (98.71%), Postives = 307/311 (98.71%), Query Frame = 0


Query: 1   MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 60
           MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW
Sbjct: 60  MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 119

Query: 61  IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 120
           IKGRISLDMQVKNILNLPEHFPQHSY VEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS
Sbjct: 120 IKGRISLDMQVKNILNLPEHFPQHSYKVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 179

Query: 121 LKKKKSKLFAQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP 180
            KKKKSKLFAQSVEDI EDESRTSKRRISISHFRSSN AATDAKFIYSSSPGNNSGFRTP
Sbjct: 180 SKKKKSKLFAQSVEDIAEDESRTSKRRISISHFRSSNTAATDAKFIYSSSPGNNSGFRTP 239

Query: 181 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 240
           PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD
Sbjct: 240 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 299

Query: 241 EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI 300
           EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI
Sbjct: 300 EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI 359

Query: 301 KRRNSISNGVC 312
           KRRNSISNGVC
Sbjct: 360 KRRNSISNGVC 370

[Close]

BLAST of Carg09258 vs. NCBI nr
Match: XP_022962218.1 (protein BIG GRAIN 1-like E [Cucurbita moschata])

HSP 1 Score: 591.3 bits (1523), Expect = 5.0e-165
Identity = 306/311 (98.39%), Postives = 306/311 (98.39%), Query Frame = 0


Query: 1   MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 60
           MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMN F  KFTPKVKKKDKGW
Sbjct: 60  MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNSFSTKFTPKVKKKDKGW 119

Query: 61  IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 120
           IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS
Sbjct: 120 IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 179

Query: 121 LKKKKSKLFAQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP 180
            KKKKSKLFAQSVEDI EDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP
Sbjct: 180 SKKKKSKLFAQSVEDIAEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP 239

Query: 181 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 240
           PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD
Sbjct: 240 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 299

Query: 241 EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI 300
           EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI
Sbjct: 300 EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI 359

Query: 301 KRRNSISNGVC 312
           KRRNSISNGVC
Sbjct: 360 KRRNSISNGVC 370

[Close]

BLAST of Carg09258 vs. NCBI nr
Match: XP_023546955.1 (protein BIG GRAIN 1-like E [Cucurbita pepo subsp. pepo])

HSP 1 Score: 591.3 bits (1523), Expect = 5.0e-165
Identity = 308/312 (98.72%), Postives = 308/312 (98.72%), Query Frame = 0


Query: 1   MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 60
           MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFG KFTPKVKKKDKGW
Sbjct: 27  MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGTKFTPKVKKKDKGW 86

Query: 61  IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 120
           IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS
Sbjct: 87  IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 146

Query: 121 LKKKKSKLFAQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP 180
            KKKKSKLFAQSVEDI EDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP
Sbjct: 147 SKKKKSKLFAQSVEDIAEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP 206

Query: 181 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 240
           PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD
Sbjct: 207 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 266

Query: 241 EEMKKKLKKFEHDINIIDDIDD-DDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDS 300
           EEMKKKLKKFEHDINIIDDIDD DDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDS
Sbjct: 267 EEMKKKLKKFEHDINIIDDIDDIDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDS 326

Query: 301 IKRRNSISNGVC 312
           IKRRNSISNGVC
Sbjct: 327 IKRRNSISNGVC 338

[Close]

BLAST of Carg09258 vs. NCBI nr
Match: XP_038886413.1 (protein BIG GRAIN 1-like E [Benincasa hispida])

HSP 1 Score: 496.9 bits (1278), Expect = 1.3e-136
Identity = 276/335 (82.39%), Postives = 293/335 (87.46%), Query Frame = 0


Query: 1   MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 60
           MSLSSS+MINKKMFHHRRASKELEVFEAARYFSDYNETSA+ N FGAKFTPKVKKKDKGW
Sbjct: 1   MSLSSSDMINKKMFHHRRASKELEVFEAARYFSDYNETSASTNSFGAKFTPKVKKKDKGW 60

Query: 61  IKGRISLDMQVKNILNLPEHFPQH-SYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHS 120
           IKGRISLDMQVKNILNLP+HFPQH SY+VEK VTKEKKYKQPSSPGGRLASFLNSLFSHS
Sbjct: 61  IKGRISLDMQVKNILNLPQHFPQHNSYSVEKQVTKEKKYKQPSSPGGRLASFLNSLFSHS 120

Query: 121 SLKKKKSKLFAQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPG-NNSGFR 180
           S KKKKSK FAQS++D+ EDESRTSKRRISISHFR+SNA ATDAKFIYSSSPG NNSGFR
Sbjct: 121 SSKKKKSKHFAQSMDDMDEDESRTSKRRISISHFRTSNAVATDAKFIYSSSPGNNNSGFR 180

Query: 181 TPPPHVQTPTKSYKELLSFSKFTRHVKSAETLEK----------------RASKQKKKNL 240
           TPPPHVQTPTKSYKELLSFSKF R VKSAE L+K                +  K KKKNL
Sbjct: 181 TPPPHVQTPTKSYKELLSFSKFNRLVKSAEALQKPTMDDKRIRKDEGVVEKLQKIKKKNL 240

Query: 241 S---NCSSEKDRVWVEKPLGDEEMKKKLKKFEHDI-NIIDD---IDDDDGGETDSSSDLF 300
           S   NCSSEKDRVWVEK L  EEMKKKL+K++H+I NIIDD    +DDDGGETDSSSDLF
Sbjct: 241 SNNNNCSSEKDRVWVEKNLVGEEMKKKLRKYDHEINNIIDDHNHEEDDDGGETDSSSDLF 300

Query: 301 ELQIYDLDYYSNGLPVYESTDIDSIKRRNSISNGV 311
           ELQIYDLDYY++GLPVYESTDIDSIKRRNSISNGV
Sbjct: 301 ELQIYDLDYYTHGLPVYESTDIDSIKRRNSISNGV 335

[Close]

BLAST of Carg09258 vs. ExPASy Swiss-Prot
Match: Q93Z37 (Protein BIG GRAIN 1-like E OS=Arabidopsis thaliana OX=3702 GN=At1g69160 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.2e-33
Identity = 118/329 (35.87%), Postives = 179/329 (54.41%), Query Frame = 0


Query: 2   SLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDK--- 61
           S  S ++  +    H+R S+EL+VFEAA YF  YNE S+  +G   K+     +++    
Sbjct: 8   SAESDKLSRRISLTHKRNSEELDVFEAAVYFG-YNEASSGDHGHTQKYGYNAAREENPRR 67

Query: 62  -GWIKG--RISLDMQVK---NILNL-PEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASF 121
            G + G  RISLD+ ++    + +L  +H  +H     K      ++KQPSSPGG++ASF
Sbjct: 68  WGILGGGRRISLDLPIRCSEQVYHLQQDHHEKHEVTTIKERLGNVRHKQPSSPGGKIASF 127

Query: 122 LNSLFSHSSLKKKKSKLFAQS-------VEDITEDESRTSKRRISISHFRSSN------- 181
           LNSLF  +  KK KSK  +++        E+I        +RR SISHF SS+       
Sbjct: 128 LNSLFHQAGSKKNKSKSKSKTKPTDPEVEEEIPGGGWMRRRRRSSISHFFSSSRSTSTTT 187

Query: 182 --AAATDAKFIYSSSPGNNSGFRTPPPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRAS 241
              A++ +K + SSS   +SGFRTPPP++ TPTK+YK+ L+++  T+ V   ET   +  
Sbjct: 188 TTTASSSSKSLISSS---SSGFRTPPPYLNTPTKNYKQFLNYTSATKQVGEEETKTNKEY 247

Query: 242 K--QKKKNLSNCSSEKDRVWVEKPLGDEEMKKKLKKFEHDINIIDDIDDDDGGETDSSSD 301
               +K  +    SE  R+W +    D++ + K +             +DDG E+DSSSD
Sbjct: 248 SWLDEKLKVMESLSENQRIWSDDEDIDDDRRIKRE------------GEDDGMESDSSSD 307

Query: 302 LFELQIYDLDYYSNGLPVYESTDIDSIKR 303
           LFELQ Y+L     GLPVYE+T++ +I +
Sbjct: 308 LFELQNYELS--RGGLPVYETTNVANINK 318

[Close]

BLAST of Carg09258 vs. ExPASy Swiss-Prot
Match: Q9SLL2 (Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.7e-04
Identity = 85/303 (28.05%), Postives = 129/303 (42.57%), Query Frame = 0


Query: 20  SKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGWIKGRISLDMQVKNILNLPE 79
           SK L+  E   +F   + +S+  +GF +  +    ++ K      IS    ++  +   E
Sbjct: 74  SKNLKTIEPV-FFKHSSSSSSDSSGFSSSESDYFYRRSKS--SPAISHPKPIRTTVERFE 133

Query: 80  HFPQ----HSYNVEKH----VTKE---------KKYKQPSSPGGRLASFLNSLFSHSSLK 139
             PQ    +S N ++H     TK          KK KQP SPGGRLA+FLNS+F+ +   
Sbjct: 134 RSPQNHRPNSSNKQEHGSFLKTKSKALKIYSDLKKVKQPISPGGRLATFLNSIFTGAGNT 193

Query: 140 KKKSKLFAQSVEDITEDESRTSKRRI-SISHFRSSNAAATDAKFIYSSSPGNNSGFRTPP 199
           KK +K+            + +S     S S F  S  + T      SSS  +    R  P
Sbjct: 194 KKLNKINTTVTSTTAAAAAASSTTTCSSASSFSRSCLSKTP-----SSSEKSKRSVRFCP 253

Query: 200 PHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVE--KPLG 259
            +V     S       SK+           +R  +  +  L N   E++R  +E  K L 
Sbjct: 254 VNVIFDEDS-------SKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRVIEAAKELL 313

Query: 260 DEEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFE---LQIYDLDYYSNGLPVYESTD 300
               KK  +  E  +   D+ DDDD   + +SSDLFE   L    +D Y   LPVYE+T 
Sbjct: 314 RSYQKKNKEVIEVSVE-DDEEDDDDDALSCTSSDLFELDNLSAIGIDRYREELPVYETTR 360

[Close]

BLAST of Carg09258 vs. ExPASy Swiss-Prot
Match: Q9LYH0 (Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.5e-04
Identity = 77/296 (26.01%), Postives = 119/296 (40.20%), Query Frame = 0


Query: 10  NKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGWIKGRISLDM 69
           +K +FH  RA+         R + DY+  S           PK  + D+ W   R     
Sbjct: 108 DKTLFHQNRAT---------RVYDDYDYASD---------VPKFNRHDENWENTR----- 167

Query: 70  QVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSSLKKKKSKLF 129
                          +    K    +KK K P+SPGGR+ +FLNSLFS++S +    K +
Sbjct: 168 ---------------NRRSVKSSGNQKKPKTPASPGGRIVNFLNSLFSNNSKQSNAVKSY 227

Query: 130 AQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTPPPHVQTPTK 189
            +     T  +     R+ S  +  S+   ++ + F  S     N G+      ++   +
Sbjct: 228 PRK----TSYDDSAYVRKTSNDYHSSTTTCSSASSFSRSCM---NKGYEKSSGRIKRSVR 287

Query: 190 -SYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD-----EEM 249
            S   ++    FT   +   +    A K  KKN+ +           + L D     E  
Sbjct: 288 FSPVNVIVPESFTSKEEDYFS-NGNARKSVKKNVEDGGRRSVEEIAREFLRDYHKNHENS 347

Query: 250 KKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQI----YDLDYYSNGLPVYEST 296
             K   FE D    D+ DDDD   +DSSSDLFEL +    +  + Y + LPVYE+T
Sbjct: 348 LVKTNGFE-DYEDDDEDDDDDDVASDSSSDLFELDLVGNHHHHNVYGDELPVYETT 356

[Close]

BLAST of Carg09258 vs. ExPASy TrEMBL
Match: A0A6J1KD53 (protein BIG GRAIN 1-like E OS=Cucurbita maxima OX=3661 GN=LOC111492162 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 4.8e-166
Identity = 307/311 (98.71%), Postives = 307/311 (98.71%), Query Frame = 0


Query: 1   MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 60
           MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW
Sbjct: 60  MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 119

Query: 61  IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 120
           IKGRISLDMQVKNILNLPEHFPQHSY VEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS
Sbjct: 120 IKGRISLDMQVKNILNLPEHFPQHSYKVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 179

Query: 121 LKKKKSKLFAQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP 180
            KKKKSKLFAQSVEDI EDESRTSKRRISISHFRSSN AATDAKFIYSSSPGNNSGFRTP
Sbjct: 180 SKKKKSKLFAQSVEDIAEDESRTSKRRISISHFRSSNTAATDAKFIYSSSPGNNSGFRTP 239

Query: 181 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 240
           PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD
Sbjct: 240 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 299

Query: 241 EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI 300
           EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI
Sbjct: 300 EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI 359

Query: 301 KRRNSISNGVC 312
           KRRNSISNGVC
Sbjct: 360 KRRNSISNGVC 370

[Close]

BLAST of Carg09258 vs. ExPASy TrEMBL
Match: A0A6J1HE67 (protein BIG GRAIN 1-like E OS=Cucurbita moschata OX=3662 GN=LOC111462734 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.4e-165
Identity = 306/311 (98.39%), Postives = 306/311 (98.39%), Query Frame = 0


Query: 1   MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 60
           MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMN F  KFTPKVKKKDKGW
Sbjct: 60  MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNSFSTKFTPKVKKKDKGW 119

Query: 61  IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 120
           IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS
Sbjct: 120 IKGRISLDMQVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSS 179

Query: 121 LKKKKSKLFAQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP 180
            KKKKSKLFAQSVEDI EDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP
Sbjct: 180 SKKKKSKLFAQSVEDIAEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTP 239

Query: 181 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 240
           PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD
Sbjct: 240 PPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD 299

Query: 241 EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI 300
           EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI
Sbjct: 300 EEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYYSNGLPVYESTDIDSI 359

Query: 301 KRRNSISNGVC 312
           KRRNSISNGVC
Sbjct: 360 KRRNSISNGVC 370

[Close]

BLAST of Carg09258 vs. ExPASy TrEMBL
Match: A0A0A0KD85 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G366580 PE=3 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.5e-130
Identity = 266/338 (78.70%), Postives = 285/338 (84.32%), Query Frame = 0


Query: 1   MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 60
           MSLSSSE+INKKMFHHRRASKEL+VFEAARYFSDYNETSA+ + FGAKFTPK+KKKDKGW
Sbjct: 1   MSLSSSEIINKKMFHHRRASKELDVFEAARYFSDYNETSASTSSFGAKFTPKMKKKDKGW 60

Query: 61  IKGRISLDMQVKNILNLPEHFPQH-SYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHS 120
           IKGRISLDMQVKNILNLP+HFPQH SY+VEK VTKEKKYKQPSSPGGRLASFLNS+FSHS
Sbjct: 61  IKGRISLDMQVKNILNLPQHFPQHDSYSVEKQVTKEKKYKQPSSPGGRLASFLNSIFSHS 120

Query: 121 SLKKKKSKLFAQSV-EDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSP-GNNSGF 180
           S KKKKSK FAQS+ ED+ +DESRTSKRRISISHFR+SNA ATDAKFIYSSSP  NNSGF
Sbjct: 121 SSKKKKSKHFAQSMDEDMEDDESRTSKRRISISHFRTSNATATDAKFIYSSSPRNNNSGF 180

Query: 181 RTPPPHVQTPTKSYKELLSFSKFTRHVKSAETLEK---------------RASKQKKKNL 240
           RTPPPHVQTPTKSYKELLSFSKF R VKSAE LEK                  K KK NL
Sbjct: 181 RTPPPHVQTPTKSYKELLSFSKFNRLVKSAEALEKPSMDDKRIRKDKGVVEKQKMKKNNL 240

Query: 241 S--NCSSEKDRVWVEKPLGDEEMKKKLKKFEHDIN--------IIDDIDDDDGGETDSSS 300
           S  NC SEKDRVWVEK L   EMKKKL+KF+H+IN          +D ++DDGGETDSSS
Sbjct: 241 SNNNCCSEKDRVWVEKNLVGGEMKKKLRKFDHEINKNNTVGGHNNNDDEEDDGGETDSSS 300

Query: 301 DLFELQIYDLDYYSNGLPVYESTDIDSIKRRNSISNGV 311
           DLFELQIYDLDYYSNGLPVYESTDIDSIKRRNS+SN V
Sbjct: 301 DLFELQIYDLDYYSNGLPVYESTDIDSIKRRNSVSNAV 338

[Close]

BLAST of Carg09258 vs. ExPASy TrEMBL
Match: A0A5A7VEM6 (Protein BIG GRAIN 1-like E OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00810 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 8.4e-118
Identity = 247/325 (76.00%), Postives = 259/325 (79.69%), Query Frame = 0


Query: 1   MSLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGW 60
           MSLSSSE INKKMFHHRRASKEL+VFEAARYFSDYNETSA+ + FGAKFTPK+KKKDKGW
Sbjct: 1   MSLSSSETINKKMFHHRRASKELDVFEAARYFSDYNETSASTSSFGAKFTPKMKKKDKGW 60

Query: 61  IKGRISLDMQVKNILNLPEHFPQH-SYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHS 120
           IKGRISLDMQVKNILNLP+HFPQH SY+VEK VTKEKKYKQPSSPGGRLASFLNSLFSHS
Sbjct: 61  IKGRISLDMQVKNILNLPQHFPQHDSYSVEKQVTKEKKYKQPSSPGGRLASFLNSLFSHS 120

Query: 121 SLKKKKSKLFAQSV-EDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSP-GNNSGF 180
           S KKKKSK FAQS+ ED+ +DESRTSKRRISISHFR+SNA ATDAKFIYSSSP  NNSGF
Sbjct: 121 SSKKKKSKHFAQSMDEDMEDDESRTSKRRISISHFRTSNATATDAKFIYSSSPKNNNSGF 180

Query: 181 RTPPPHVQTPTKSYKELLSFSKFTRHVKSAETLE------KRASKQK------KKNLSNC 240
           RTPPPHVQTPTKSYKELLSFSKF R VKSAE LE      KR  K K      K   +NC
Sbjct: 181 RTPPPHVQTPTKSYKELLSFSKFNRLVKSAEALEKPTMDDKRIRKDKGVVEKQKMKKNNC 240

Query: 241 SSEKDRVWVEKPLGDEEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQIYDLDYY 300
            SEKDRVWVEK L                              DSSSDLFELQIYDLDYY
Sbjct: 241 CSEKDRVWVEKNL-----------------------------VDSSSDLFELQIYDLDYY 296

Query: 301 SNGLPVYESTDIDSIKRRNSISNGV 311
           SNGLPVYESTDIDSIKRRNS+SN V
Sbjct: 301 SNGLPVYESTDIDSIKRRNSVSNAV 296

[Close]

BLAST of Carg09258 vs. ExPASy TrEMBL
Match: A0A6J1BRW3 (protein BIG GRAIN 1-like E OS=Momordica charantia OX=3673 GN=LOC111004991 PE=3 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 1.2e-76
Identity = 202/333 (60.66%), Postives = 221/333 (66.37%), Query Frame = 0


Query: 8   MINKKMFHHRRASK-ELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKD-KGWIKGRI 67
           M+NKKMFHHRRASK +LE FEAA                 AKFT KVKKKD KGWIKGRI
Sbjct: 1   MMNKKMFHHRRASKDQLEAFEAA--------------ASAAKFTQKVKKKDHKGWIKGRI 60

Query: 68  SLDMQVKNILNLPEHFPQHSYN---VEKHVTKEKKYK-QPSSPGGRLASFLNSLFSH--- 127
           SLDMQVKNILNLP+H   HS +   V K +   K  K QPSSPGGRLA+FLNSLFSH   
Sbjct: 61  SLDMQVKNILNLPQHNNSHSVDQKQVVKDIDHNKNLKQQPSSPGGRLANFLNSLFSHSSG 120

Query: 128 SSLKKKKSKLFAQSVEDITE----DESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNN 187
           SS KK K K   QS+ED+ +       R SKRRISISH       +   KFIYSSSP   
Sbjct: 121 SSKKKNKPKSSLQSMEDMEQLHQSPRGRNSKRRISISH-------SDHTKFIYSSSP--P 180

Query: 188 SGFRTPP-PHVQTPTKSYKELLSFSKFTRHVKSAETLEKR--------------ASKQKK 247
           SGF TPP   VQTPTK YKELLSFSK  RH++SA  L+K                 KQKK
Sbjct: 181 SGFGTPPRDQVQTPTKRYKELLSFSKLNRHIRSAVALQKPGVDEEKRRKDEAGCVEKQKK 240

Query: 248 KNLSNCSSEKDRVWVEKPLGDEEMKKKLKKFEHDI-NIIDDIDDDDGGETDSSSDLFELQ 307
           K   NC  EKDR WVEK + +E    +L+K +  I NIID  DDDDGGETDSSSDLFELQ
Sbjct: 241 KKKKNC-GEKDRGWVEKMVAEE---IELRKIDDIIDNIID--DDDDGGETDSSSDLFELQ 300

Query: 308 IYDLDYYSNGLPVYESTDIDSIKRRNSISNGVC 312
           IYDLDYYS GLPVYESTDID+IKRRNSISNGVC
Sbjct: 301 IYDLDYYSTGLPVYESTDIDTIKRRNSISNGVC 304

[Close]

BLAST of Carg09258 vs. TAIR 10
Match: AT1G69160.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 173 Blast hits to 172 proteins in 54 species: Archae - 0; Bacteria - 0; Metazoa - 25; Fungi - 33; Plants - 84; Viruses - 2; Other Eukaryotes - 29 (source: NCBI BLink). )

HSP 1 Score: 145.2 bits (365), Expect = 8.8e-35
Identity = 118/329 (35.87%), Postives = 179/329 (54.41%), Query Frame = 0


Query: 2   SLSSSEMINKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDK--- 61
           S  S ++  +    H+R S+EL+VFEAA YF  YNE S+  +G   K+     +++    
Sbjct: 8   SAESDKLSRRISLTHKRNSEELDVFEAAVYFG-YNEASSGDHGHTQKYGYNAAREENPRR 67

Query: 62  -GWIKG--RISLDMQVK---NILNL-PEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASF 121
            G + G  RISLD+ ++    + +L  +H  +H     K      ++KQPSSPGG++ASF
Sbjct: 68  WGILGGGRRISLDLPIRCSEQVYHLQQDHHEKHEVTTIKERLGNVRHKQPSSPGGKIASF 127

Query: 122 LNSLFSHSSLKKKKSKLFAQS-------VEDITEDESRTSKRRISISHFRSSN------- 181
           LNSLF  +  KK KSK  +++        E+I        +RR SISHF SS+       
Sbjct: 128 LNSLFHQAGSKKNKSKSKSKTKPTDPEVEEEIPGGGWMRRRRRSSISHFFSSSRSTSTTT 187

Query: 182 --AAATDAKFIYSSSPGNNSGFRTPPPHVQTPTKSYKELLSFSKFTRHVKSAETLEKRAS 241
              A++ +K + SSS   +SGFRTPPP++ TPTK+YK+ L+++  T+ V   ET   +  
Sbjct: 188 TTTASSSSKSLISSS---SSGFRTPPPYLNTPTKNYKQFLNYTSATKQVGEEETKTNKEY 247

Query: 242 K--QKKKNLSNCSSEKDRVWVEKPLGDEEMKKKLKKFEHDINIIDDIDDDDGGETDSSSD 301
               +K  +    SE  R+W +    D++ + K +             +DDG E+DSSSD
Sbjct: 248 SWLDEKLKVMESLSENQRIWSDDEDIDDDRRIKRE------------GEDDGMESDSSSD 307

Query: 302 LFELQIYDLDYYSNGLPVYESTDIDSIKR 303
           LFELQ Y+L     GLPVYE+T++ +I +
Sbjct: 308 LFELQNYELS--RGGLPVYETTNVANINK 318

[Close]

BLAST of Carg09258 vs. TAIR 10
Match: AT1G54200.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1084 Blast hits to 581 proteins in 136 species: Archae - 0; Bacteria - 72; Metazoa - 212; Fungi - 78; Plants - 102; Viruses - 0; Other Eukaryotes - 620 (source: NCBI BLink). )

HSP 1 Score: 47.8 bits (112), Expect = 1.9e-05
Identity = 85/303 (28.05%), Postives = 129/303 (42.57%), Query Frame = 0


Query: 20  SKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGWIKGRISLDMQVKNILNLPE 79
           SK L+  E   +F   + +S+  +GF +  +    ++ K      IS    ++  +   E
Sbjct: 74  SKNLKTIEPV-FFKHSSSSSSDSSGFSSSESDYFYRRSKS--SPAISHPKPIRTTVERFE 133

Query: 80  HFPQ----HSYNVEKH----VTKE---------KKYKQPSSPGGRLASFLNSLFSHSSLK 139
             PQ    +S N ++H     TK          KK KQP SPGGRLA+FLNS+F+ +   
Sbjct: 134 RSPQNHRPNSSNKQEHGSFLKTKSKALKIYSDLKKVKQPISPGGRLATFLNSIFTGAGNT 193

Query: 140 KKKSKLFAQSVEDITEDESRTSKRRI-SISHFRSSNAAATDAKFIYSSSPGNNSGFRTPP 199
           KK +K+            + +S     S S F  S  + T      SSS  +    R  P
Sbjct: 194 KKLNKINTTVTSTTAAAAAASSTTTCSSASSFSRSCLSKTP-----SSSEKSKRSVRFCP 253

Query: 200 PHVQTPTKSYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVE--KPLG 259
            +V     S       SK+           +R  +  +  L N   E++R  +E  K L 
Sbjct: 254 VNVIFDEDS-------SKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRVIEAAKELL 313

Query: 260 DEEMKKKLKKFEHDINIIDDIDDDDGGETDSSSDLFE---LQIYDLDYYSNGLPVYESTD 300
               KK  +  E  +   D+ DDDD   + +SSDLFE   L    +D Y   LPVYE+T 
Sbjct: 314 RSYQKKNKEVIEVSVE-DDEEDDDDDALSCTSSDLFELDNLSAIGIDRYREELPVYETTR 360

[Close]

BLAST of Carg09258 vs. TAIR 10
Match: AT5G12050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 47.4 bits (111), Expect = 2.5e-05
Identity = 77/296 (26.01%), Postives = 119/296 (40.20%), Query Frame = 0


Query: 10  NKKMFHHRRASKELEVFEAARYFSDYNETSATMNGFGAKFTPKVKKKDKGWIKGRISLDM 69
           +K +FH  RA+         R + DY+  S           PK  + D+ W   R     
Sbjct: 108 DKTLFHQNRAT---------RVYDDYDYASD---------VPKFNRHDENWENTR----- 167

Query: 70  QVKNILNLPEHFPQHSYNVEKHVTKEKKYKQPSSPGGRLASFLNSLFSHSSLKKKKSKLF 129
                          +    K    +KK K P+SPGGR+ +FLNSLFS++S +    K +
Sbjct: 168 ---------------NRRSVKSSGNQKKPKTPASPGGRIVNFLNSLFSNNSKQSNAVKSY 227

Query: 130 AQSVEDITEDESRTSKRRISISHFRSSNAAATDAKFIYSSSPGNNSGFRTPPPHVQTPTK 189
            +     T  +     R+ S  +  S+   ++ + F  S     N G+      ++   +
Sbjct: 228 PRK----TSYDDSAYVRKTSNDYHSSTTTCSSASSFSRSCM---NKGYEKSSGRIKRSVR 287

Query: 190 -SYKELLSFSKFTRHVKSAETLEKRASKQKKKNLSNCSSEKDRVWVEKPLGD-----EEM 249
            S   ++    FT   +   +    A K  KKN+ +           + L D     E  
Sbjct: 288 FSPVNVIVPESFTSKEEDYFS-NGNARKSVKKNVEDGGRRSVEEIAREFLRDYHKNHENS 347

Query: 250 KKKLKKFEHDINIIDDIDDDDGGETDSSSDLFELQI----YDLDYYSNGLPVYEST 296
             K   FE D    D+ DDDD   +DSSSDLFEL +    +  + Y + LPVYE+T
Sbjct: 348 LVKTNGFE-DYEDDDEDDDDDDVASDSSSDLFELDLVGNHHHHNVYGDELPVYETT 356

[Close]

BLAST of Carg09258 vs. TAIR 10
Match: AT3G13980.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 1485 Blast hits to 418 proteins in 98 species: Archae - 0; Bacteria - 6; Metazoa - 246; Fungi - 61; Plants - 107; Viruses - 6; Other Eukaryotes - 1059 (source: NCBI BLink). )

HSP 1 Score: 45.4 bits (106), Expect = 9.4e-05
Identity = 86/332 (25.90%), Postives = 138/332 (41.57%), Query Frame = 0


Query: 7   EMINKKMFHHRRASKELEV----------------FEAARYFSDY----NETSATMNGFG 66
           E I KK  HH++ +  L                  FE +R  +D+    N +S+  +GF 
Sbjct: 41  ESIKKKKHHHQQRNASLHEDREISPIYHRRSIAADFERSRRKTDFLRHSNSSSSDSSGFS 100

Query: 67  AKFTPKVKKKDKGWIKGRISLDMQVKNI----------LNLPEH---FPQHSYNVEKHVT 126
           +  +     + K       S   Q K I          +  P+    F +      K  +
Sbjct: 101 SSESDSFHGRSKSSASPPSSSRQQPKPIRTSSVDHSSAVQKPKELGGFLRTKSKALKIYS 160

Query: 127 KEKKYKQPSSPGGRLASFLNSLFSHSSLKKKKSKLFAQSVEDITEDESRTSKRRISISHF 186
             KK KQP SPGGRLA+FLNSLF++++   KK K   ++      +E  +S    S S F
Sbjct: 161 DLKKVKQPISPGGRLATFLNSLFTNAATNPKKHK---KTTTVAVVEEPHSSSTCSSASSF 220

Query: 187 RSSNAAATDAKFIYSSSPGNNSGFRTPPPHVQTPTKSYKELLSFSKFTRHVKSAETLEKR 246
             S  + T      SSS  +    R  P +V     S     SF+       +    +  
Sbjct: 221 SRSCLSKTP-----SSSGKSKRSVRFCPVNVILDEDS-----SFT-MPYAYNNERLYDNN 280

Query: 247 ASKQKKKNLSNCSSEKDRVWVEKPLGDEEMKKKLKKFEHDINIIDDIDDDDGGETDSSSD 303
            +K+ +++     + KD +             K K    +IN +++ DD+D   + +SSD
Sbjct: 281 EAKRVEEHRRVIQAAKDLL--------RTYHNKNKVTTTNINNVEE-DDEDDAASCASSD 340

The following BLAST results are available for this feature:

BLAST of Carg09258 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6598646.1	3.7e-168	100.00	Protein BIG GRAIN 1-like E, partial [Cucurbita argyrosperma subsp. sororia] >KAG...	[more]
XP_022997188.1	1.0e-165	98.71	protein BIG GRAIN 1-like E [Cucurbita maxima]	[more]
XP_022962218.1	5.0e-165	98.39	protein BIG GRAIN 1-like E [Cucurbita moschata]	[more]
XP_023546955.1	5.0e-165	98.72	protein BIG GRAIN 1-like E [Cucurbita pepo subsp. pepo]	[more]
XP_038886413.1	1.3e-136	82.39	protein BIG GRAIN 1-like E [Benincasa hispida]	[more]

BLAST of Carg09258 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93Z37	1.2e-33	35.87	Protein BIG GRAIN 1-like E OS=Arabidopsis thaliana OX=3702 GN=At1g69160 PE=2 SV=...	[more]
Q9SLL2	2.7e-04	28.05	Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=...	[more]
Q9LYH0	3.5e-04	26.01	Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=...	[more]

BLAST of Carg09258 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KD53	4.8e-166	98.71	protein BIG GRAIN 1-like E OS=Cucurbita maxima OX=3661 GN=LOC111492162 PE=3 SV=1	[more]
A0A6J1HE67	2.4e-165	98.39	protein BIG GRAIN 1-like E OS=Cucurbita moschata OX=3662 GN=LOC111462734 PE=3 SV...	[more]
A0A0A0KD85	1.5e-130	78.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G366580 PE=3 SV=1	[more]
A0A5A7VEM6	8.4e-118	76.00	Protein BIG GRAIN 1-like E OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A6J1BRW3	1.2e-76	60.66	protein BIG GRAIN 1-like E OS=Momordica charantia OX=3673 GN=LOC111004991 PE=3 S...	[more]

BLAST of Carg09258 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69160.1	8.8e-35	35.87	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G54200.1	1.9e-05	28.05	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G12050.1	2.5e-05	26.01	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT3G13980.1	9.4e-05	25.90	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33541:SF11	PROTEIN BIG GRAIN 1-LIKE E	coord: 3..310
IPR039621	Protein BIG GRAIN 1-like	PANTHER	PTHR33541	PROTEIN BIG GRAIN 1-LIKE A-RELATED	coord: 3..310

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09258-RA	Carg09258-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009734	auxin-activated signaling pathway
cellular_component	GO:0005886	plasma membrane