Carg06021 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06021
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	aspartic proteinase PCS1-like
Location	Carg_Chr04: 21561283 .. 21562572 (+)
RNA-Seq Expression	Carg06021
Synteny	Carg06021

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACTTCTTCCTCCACCTTCTGCAACTCCTCGTCTTCCTCCTCTGTTTCAAGCAGAGTCTCTGTTTTTCTGCAACTCAGGCGATGGTTTTGCCACTCAAAACAGAGACGGGAGTGAATTCACAGCCGTCGAACAAACTCAGTTTCCACCATAATGTCACGTTGACCGTTGAGTTGACTGTCGGATCGCCGCCTCAAGCTGTCACGATGGTGCTGGATACAGGGAGTGAACTCCCCTGGCTTAACTGCAAAAAAAAAACCCAAACTTTGACCTCTGTTTTTAACCCACTTTCTTCCTCTTCTTACTCGCGAATCCCCTGCATTTCCCCCATTTGTCAGAAGCAAACCCGAGACTTACCCAACCCGGTTGTATGCGACGAAGAAAAACTATGCAATGTCTTTGTCTCATACGCTGACGGCTCGTCTCTCGAGGGTAATCTTGCATCCGACACGTTTCGAATCGGGTCGTTGAATAAACCCGGAACTTTATTCGGGTGTATGGGTTTGGGTTCGAGTTCGAATCCGCAGGAGGATGCGAAGACCACTGGGCTAATGGGGATGAACCGGGGGTCGCTTTCGTTTGTGACTCAATTGGGTCTGTCGAAATTCTCTTACTGCATATCGGGTCGTGATTCCTCCGGCGTTCTGATTTTAGGCGAAGGTAATCATTCGTGGGTTGGGAATATGGCGTACACGCCGTTGGTTCAAATGTCGACGCCATTGCCGTCGTACGATCGGTTCGCGTACACAGTCAAACTGGAGGGAATTAAAGTGGGGAACAAAATCCTGGCGCTGGAGAAATCAATTCTGGTTCCGGACCACACCGGCGCCGGGCAGACCATGGTGGATTCCGGGACCCAGTTCACGTTTCTTCTGGGGCCGGTGTACACGGCTTTAAAGAACGAGTTTCTGGTACAAACGAAGGGCGTTTTGGTTCCACTGGGTGATCCGAATTTCGTGTTCCAAGGAGCATTGGACTCGTGCTTCCGAGTACCGGCGAATAAGGGGAAGCTTCCGCCGCTGCCGACGGTGAGTCTAATGTTGAATGGGGCGGAGATGGTTGTGGGCGAGGAGTTGCTGCTGTACCGAGTGCCGGGAATGGTGAAGGGTGGTGATTGGGTGTATTGCATGACGTTTGGGAACTCGGATTTGTTGGGAATAGCGGCGTTTGTGATTGGGAATCATCATCAACAGAACTTGTGGATGGAATATGATCTGGCGAAATCAAGGCTGGGATTTGTCGAGACCAGGTGTGATTCAGCGGGTCAACGCCTCGGATTGGACCTTTAA

mRNA sequence

ATGAACTTCTTCCTCCACCTTCTGCAACTCCTCGTCTTCCTCCTCTGTTTCAAGCAGAGTCTCTGTTTTTCTGCAACTCAGGCGATGGTTTTGCCACTCAAAACAGAGACGGGAGTGAATTCACAGCCGTCGAACAAACTCAGTTTCCACCATAATGTCACGTTGACCGTTGAGTTGACTGTCGGATCGCCGCCTCAAGCTGTCACGATGGTGCTGGATACAGGGAGTGAACTCCCCTGGCTTAACTGCAAAAAAAAAACCCAAACTTTGACCTCTGTTTTTAACCCACTTTCTTCCTCTTCTTACTCGCGAATCCCCTGCATTTCCCCCATTTGTCAGAAGCAAACCCGAGACTTACCCAACCCGGTTGTATGCGACGAAGAAAAACTATGCAATGTCTTTGTCTCATACGCTGACGGCTCGTCTCTCGAGGGTAATCTTGCATCCGACACGTTTCGAATCGGGTCGTTGAATAAACCCGGAACTTTATTCGGGTGTATGGGTTTGGGTTCGAGTTCGAATCCGCAGGAGGATGCGAAGACCACTGGGCTAATGGGGATGAACCGGGGGTCGCTTTCGTTTGTGACTCAATTGGGTCTGTCGAAATTCTCTTACTGCATATCGGGTCGTGATTCCTCCGGCGTTCTGATTTTAGGCGAAGGTAATCATTCGTGGGTTGGGAATATGGCGTACACGCCGTTGGTTCAAATGTCGACGCCATTGCCGTCGTACGATCGGTTCGCGTACACAGTCAAACTGGAGGGAATTAAAGTGGGGAACAAAATCCTGGCGCTGGAGAAATCAATTCTGGTTCCGGACCACACCGGCGCCGGGCAGACCATGGTGGATTCCGGGACCCAGTTCACGTTTCTTCTGGGGCCGGTGTACACGGCTTTAAAGAACGAGTTTCTGGTACAAACGAAGGGCGTTTTGGTTCCACTGGGTGATCCGAATTTCGTGTTCCAAGGAGCATTGGACTCGTGCTTCCGAGTACCGGCGAATAAGGGGAAGCTTCCGCCGCTGCCGACGGTGAGTCTAATGTTGAATGGGGCGGAGATGGTTGTGGGCGAGGAGTTGCTGCTGTACCGAGTGCCGGGAATGGTGAAGGGTGGTGATTGGGTGTATTGCATGACGTTTGGGAACTCGGATTTGTTGGGAATAGCGGCGTTTGTGATTGGGAATCATCATCAACAGAACTTGTGGATGGAATATGATCTGGCGAAATCAAGGCTGGGATTTGTCGAGACCAGGTGTGATTCAGCGGGTCAACGCCTCGGATTGGACCTTTAA

Coding sequence (CDS)

ATGAACTTCTTCCTCCACCTTCTGCAACTCCTCGTCTTCCTCCTCTGTTTCAAGCAGAGTCTCTGTTTTTCTGCAACTCAGGCGATGGTTTTGCCACTCAAAACAGAGACGGGAGTGAATTCACAGCCGTCGAACAAACTCAGTTTCCACCATAATGTCACGTTGACCGTTGAGTTGACTGTCGGATCGCCGCCTCAAGCTGTCACGATGGTGCTGGATACAGGGAGTGAACTCCCCTGGCTTAACTGCAAAAAAAAAACCCAAACTTTGACCTCTGTTTTTAACCCACTTTCTTCCTCTTCTTACTCGCGAATCCCCTGCATTTCCCCCATTTGTCAGAAGCAAACCCGAGACTTACCCAACCCGGTTGTATGCGACGAAGAAAAACTATGCAATGTCTTTGTCTCATACGCTGACGGCTCGTCTCTCGAGGGTAATCTTGCATCCGACACGTTTCGAATCGGGTCGTTGAATAAACCCGGAACTTTATTCGGGTGTATGGGTTTGGGTTCGAGTTCGAATCCGCAGGAGGATGCGAAGACCACTGGGCTAATGGGGATGAACCGGGGGTCGCTTTCGTTTGTGACTCAATTGGGTCTGTCGAAATTCTCTTACTGCATATCGGGTCGTGATTCCTCCGGCGTTCTGATTTTAGGCGAAGGTAATCATTCGTGGGTTGGGAATATGGCGTACACGCCGTTGGTTCAAATGTCGACGCCATTGCCGTCGTACGATCGGTTCGCGTACACAGTCAAACTGGAGGGAATTAAAGTGGGGAACAAAATCCTGGCGCTGGAGAAATCAATTCTGGTTCCGGACCACACCGGCGCCGGGCAGACCATGGTGGATTCCGGGACCCAGTTCACGTTTCTTCTGGGGCCGGTGTACACGGCTTTAAAGAACGAGTTTCTGGTACAAACGAAGGGCGTTTTGGTTCCACTGGGTGATCCGAATTTCGTGTTCCAAGGAGCATTGGACTCGTGCTTCCGAGTACCGGCGAATAAGGGGAAGCTTCCGCCGCTGCCGACGGTGAGTCTAATGTTGAATGGGGCGGAGATGGTTGTGGGCGAGGAGTTGCTGCTGTACCGAGTGCCGGGAATGGTGAAGGGTGGTGATTGGGTGTATTGCATGACGTTTGGGAACTCGGATTTGTTGGGAATAGCGGCGTTTGTGATTGGGAATCATCATCAACAGAACTTGTGGATGGAATATGATCTGGCGAAATCAAGGCTGGGATTTGTCGAGACCAGGTGTGATTCAGCGGGTCAACGCCTCGGATTGGACCTTTAA

Protein sequence

MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELTVGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLPNPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAKTTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTPLPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELLLYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDSAGQRLGLDL

Homology

[Close]

BLAST of Carg06021 vs. NCBI nr
Match: KAG7033342.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 869.4 bits (2245), Expect = 1.3e-248
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0


Query: 1   MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELT 60
           MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELT
Sbjct: 1   MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELT 60

Query: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120
           VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP
Sbjct: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120

Query: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180
           NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK
Sbjct: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240

Query: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK
Sbjct: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300

Query: 301 NEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELL 360
           NEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELL
Sbjct: 301 NEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELL 360

Query: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420
           LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS
Sbjct: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420

Query: 421 AGQRLGLDL 430
           AGQRLGLDL
Sbjct: 421 AGQRLGLDL 429

[Close]

BLAST of Carg06021 vs. NCBI nr
Match: XP_022955370.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 861.3 bits (2224), Expect = 3.5e-246
Identity = 424/429 (98.83%), Postives = 427/429 (99.53%), Query Frame = 0


Query: 1   MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELT 60
           MNFFLHLLQLLVFLLCFKQSLCF ATQAMVLPLKTETG+NSQPSNKLSFHHNVTLTVELT
Sbjct: 1   MNFFLHLLQLLVFLLCFKQSLCFCATQAMVLPLKTETGLNSQPSNKLSFHHNVTLTVELT 60

Query: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120
           VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP
Sbjct: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120

Query: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180
           NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK
Sbjct: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240

Query: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK
Sbjct: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300

Query: 301 NEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELL 360
           NEFLVQTKGVLVPLGDPNFVFQGA+DSCFRVPAN+GKLPPLPTVSLMLNGAEMVVG ELL
Sbjct: 301 NEFLVQTKGVLVPLGDPNFVFQGAMDSCFRVPANQGKLPPLPTVSLMLNGAEMVVGGELL 360

Query: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420
           LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS
Sbjct: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420

Query: 421 AGQRLGLDL 430
           AGQRLGLDL
Sbjct: 421 AGQRLGLDL 429

[Close]

BLAST of Carg06021 vs. NCBI nr
Match: XP_023540416.1 (aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.5 bits (2196), Expect = 6.3e-243
Identity = 417/428 (97.43%), Postives = 423/428 (98.83%), Query Frame = 0


Query: 1   MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELT 60
           MNFF HLLQLLVF LCFKQ LCFSATQAMVLPLKTETG+NSQPSNK+SFHHNVTLTVELT
Sbjct: 1   MNFFFHLLQLLVFFLCFKQRLCFSATQAMVLPLKTETGLNSQPSNKISFHHNVTLTVELT 60

Query: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120
           VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP
Sbjct: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120

Query: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180
           NPVVCDEEKLC+VFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK
Sbjct: 121 NPVVCDEEKLCDVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240

Query: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDH+GAGQTMVDSGTQFTFLLGPVYTALK
Sbjct: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHSGAGQTMVDSGTQFTFLLGPVYTALK 300

Query: 301 NEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELL 360
           NEFLVQTKGVLVPLGDPNFVFQGA+DSCFRVPAN+GKLPPLP VSLMLNGAEMVVG ELL
Sbjct: 301 NEFLVQTKGVLVPLGDPNFVFQGAMDSCFRVPANQGKLPPLPAVSLMLNGAEMVVGGELL 360

Query: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420
           LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS
Sbjct: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420

Query: 421 AGQRLGLD 429
           AGQRLGLD
Sbjct: 421 AGQRLGLD 428

[Close]

BLAST of Carg06021 vs. NCBI nr
Match: KAG6602656.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 844.0 bits (2179), Expect = 5.9e-241
Identity = 415/423 (98.11%), Postives = 419/423 (99.05%), Query Frame = 0


Query: 1   MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELT 60
           MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETG+NSQPSNKLSFHHNVTLTVELT
Sbjct: 1   MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGLNSQPSNKLSFHHNVTLTVELT 60

Query: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120
           VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP
Sbjct: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120

Query: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180
           NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK
Sbjct: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240

Query: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK
Sbjct: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300

Query: 301 NEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELL 360
           NEFLVQTKGVLVPLGDPNFVFQGA+DSCFRVPANKGKLPPLPTV LMLNGAEMVVGEELL
Sbjct: 301 NEFLVQTKGVLVPLGDPNFVFQGAMDSCFRVPANKGKLPPLPTVGLMLNGAEMVVGEELL 360

Query: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420
           LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFV+GNHHQQNLWMEYDLAKSRLGFVETR D 
Sbjct: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVVGNHHQQNLWMEYDLAKSRLGFVETRRDG 420

Query: 421 AGQ 424
           + Q
Sbjct: 421 SQQ 423

[Close]

BLAST of Carg06021 vs. NCBI nr
Match: XP_022991088.1 (aspartic proteinase PCS1-like [Cucurbita maxima])

HSP 1 Score: 837.4 bits (2162), Expect = 5.5e-239
Identity = 415/429 (96.74%), Postives = 419/429 (97.67%), Query Frame = 0


Query: 1   MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELT 60
           M+FFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETG+NSQPSNKLSFHHNVTLTVELT
Sbjct: 1   MDFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGLNSQPSNKLSFHHNVTLTVELT 60

Query: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120
           VGSPPQAVTMVLDTGSELPWLNC KKTQTLTSVFNPLSSSSYSRIPC SPICQKQTRDLP
Sbjct: 61  VGSPPQAVTMVLDTGSELPWLNC-KKTQTLTSVFNPLSSSSYSRIPCSSPICQKQTRDLP 120

Query: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180
           NPVVCD EKLCNVFVSYADGSSLEGNLASDTFRIGS NKPGTLFGCMG  SSSNPQEDAK
Sbjct: 121 NPVVCDSEKLCNVFVSYADGSSLEGNLASDTFRIGSFNKPGTLFGCMGSDSSSNPQEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240

Query: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK
Sbjct: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300

Query: 301 NEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELL 360
           NEFLVQTKGVLVPL DPNFVFQGA+DSCFRVPAN+GKLPPLP VSLMLNGAEMVVG ELL
Sbjct: 301 NEFLVQTKGVLVPLDDPNFVFQGAMDSCFRVPANQGKLPPLPAVSLMLNGAEMVVGGELL 360

Query: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420
           LYRVPGMVKGGDWVYCMTFGNSDLLG AAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS
Sbjct: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGTAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420

Query: 421 AGQRLGLDL 430
           AGQRLGLDL
Sbjct: 421 AGQRLGLDL 428

[Close]

BLAST of Carg06021 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 1.9e-138
Identity = 241/411 (58.64%), Postives = 305/411 (74.21%), Query Frame = 0


Query: 24  SATQAMVLPLKTE-TGVNSQPSNKLSFHHNVTLTVELTVGSPPQAVTMVLDTGSELPWLN 83
           S++Q +VLPLKT  T  + +P++KL FHHNVTLTV LTVG+PPQ ++MV+DTGSEL WL 
Sbjct: 41  SSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELSWLR 100

Query: 84  CKKKTQ-TLTSVFNPLSSSSYSRIPCISPICQKQTRDLPNPVVCDEEKLCNVFVSYADGS 143
           C + +     + F+P  SSSYS IPC SP C+ +TRD   P  CD +KLC+  +SYAD S
Sbjct: 101 CNRSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADAS 160

Query: 144 SLEGNLASDTFRIG-SLNKPGTLFGCMGLGSSSNPQEDAKTTGLMGMNRGSLSFVTQLGL 203
           S EGNLA++ F  G S N    +FGCMG  S S+P+ED KTTGL+GMNRGSLSF++Q+G 
Sbjct: 161 SSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGF 220

Query: 204 SKFSYCISGRDS-SGVLILGEGNHSWVGNMAYTPLVQMSTPLPSYDRFAYTVKLEGIKVG 263
            KFSYCISG D   G L+LG+ N +W+  + YTPL+++STPLP +DR AYTV+L GIKV 
Sbjct: 221 PKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVN 280

Query: 264 NKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGVLVPLGDPNF 323
            K+L + KS+LVPDHTGAGQTMVDSGTQFTFLLGPVYTAL++ FL +T G+L    DP+F
Sbjct: 281 GKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDF 340

Query: 324 VFQGALDSCFR---VPANKGKLPPLPTVSLMLNGAEMVVGEELLLYRVPGMVKGGDWVYC 383
           VFQG +D C+R   V    G L  LPTVSL+  GAE+ V  + LLYRVP +  G D VYC
Sbjct: 341 VFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGNDSVYC 400

Query: 384 MTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDSAGQRLGL 428
            TFGNSDL+G+ A+VIG+HHQQN+W+E+DL +SR+G     CD +GQRLG+
Sbjct: 401 FTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGI 451

[Close]

BLAST of Carg06021 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.4e-43
Identity = 118/370 (31.89%), Postives = 181/370 (48.92%), Query Frame = 0


Query: 57  VELTVGSPPQAVTMVLDTGSELPWLNCKKKTQTL---TSVFNPLSSSSYSRIPCISPICQ 116
           + + +G+P  + + ++DTGS+L W  C+  TQ     T +FNP  SSS+S +PC S  CQ
Sbjct: 98  MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQ 157

Query: 117 KQTRDLPNPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSS 176
               DLP+    + E  C     Y DGS+ +G +A++TF   + + P   FGC   G  +
Sbjct: 158 ----DLPSETCNNNE--CQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGC---GEDN 217

Query: 177 NPQEDAKTTGLMGMNRGSLSFVTQLGLSKFSYCIS--GRDSSGVLILGEGNHSWVGNMAY 236
                    GL+GM  G LS  +QLG+ +FSYC++  G  S   L LG            
Sbjct: 218 QGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPS 277

Query: 237 TPLVQMSTPLPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFL 296
           T L+  S   P+Y    Y + L+GI VG   L +  S       G G  ++DSGT  T+L
Sbjct: 278 TTLIHSSLN-PTY----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYL 337

Query: 297 LGPVYTALKNEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGA 356
               Y A+   F   T  + +P  D +      L +CF+ P++ G    +P +S+  +G 
Sbjct: 338 PQDAYNAVAQAF---TDQINLPTVDES---SSGLSTCFQQPSD-GSTVQVPEISMQFDGG 397

Query: 357 EMVVGEELLLYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRL 416
            + +GE+ +L      +   + V C+  G+S  LGI+ F  GN  QQ   + YDL    +
Sbjct: 398 VLNLGEQNIL------ISPAEGVICLAMGSSSQLGISIF--GNIQQQETQVLYDLQNLAV 438

Query: 417 GFVETRCDSA 422
            FV T+C ++
Sbjct: 458 SFVPTQCGAS 438

[Close]

BLAST of Carg06021 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.4e-42
Identity = 111/371 (29.92%), Postives = 186/371 (50.13%), Query Frame = 0


Query: 57  VELTVGSPPQAVTMVLDTGSELPWLNCKKKTQTL---TSVFNPLSSSSYSRIPCISPICQ 116
           + L++G+P Q  + ++DTGS+L W  C+  TQ     T +FNP  SSS+S +PC S +CQ
Sbjct: 97  MNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQ 156

Query: 117 KQTRDLPNPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSS 176
                L +P        C     Y DGS  +G++ ++T   GS++ P   FGC   G ++
Sbjct: 157 A----LSSPTC--SNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGC---GENN 216

Query: 177 NPQEDAKTTGLMGMNRGSLSFVTQLGLSKFSYCIS--GRDSSGVLILGEGNHSWVGNMAY 236
                    GL+GM RG LS  +QL ++KFSYC++  G  +   L+LG   +S       
Sbjct: 217 QGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPN 276

Query: 237 TPLVQMSTPLPSYDRFAYTVKLEGIKVGNKILALEKS-ILVPDHTGAGQTMVDSGTQFTF 296
           T L+Q S+ +P++    Y + L G+ VG+  L ++ S   +  + G G  ++DSGT  T+
Sbjct: 277 TTLIQ-SSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTY 336

Query: 297 LLGPVYTALKNEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNG 356
            +   Y +++ EF+ Q    +V      F      D CF+ P++   L  +PT  +  +G
Sbjct: 337 FVNNAYQSVRQEFISQINLPVVNGSSSGF------DLCFQTPSDPSNL-QIPTFVMHFDG 396

Query: 357 AEMVVGEELLLYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSR 416
            ++ +  E         +   + + C+  G+S   G++ F  GN  QQN+ + YD   S 
Sbjct: 397 GDLELPSENY------FISPSNGLICLAMGSSS-QGMSIF--GNIQQQNMLVVYDTGNSV 437

Query: 417 LGFVETRCDSA 422
           + F   +C ++
Sbjct: 457 VSFASAQCGAS 437

[Close]

BLAST of Carg06021 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.6e-34
Identity = 122/370 (32.97%), Postives = 175/370 (47.30%), Query Frame = 0


Query: 59  LTVGSPPQAVTMVLDTGSELPWLN---CKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQ 118
           L VG+P + V MVLDTGS++ WL    C++       +F+P  S +Y+ IPC SP C   
Sbjct: 146 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHC--- 205

Query: 119 TRDLPNPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNP 178
            R L +       K C   VSY DGS   G+ +++T         G   GC       N 
Sbjct: 206 -RRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGC----GHDNE 265

Query: 179 QEDAKTTGLMGMNRGSLSFVTQLG---LSKFSYCISGRDSSGVLILGEGNHSWVGNMAYT 238
                  GL+G+ +G LSF  Q G     KFSYC+  R +S      + +    GN A +
Sbjct: 266 GLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSAS-----SKPSSVVFGNAAVS 325

Query: 239 PLVQMSTPL---PSYDRFAYTVKLEGIKV-GNKILALEKSILVPDHTGAGQTMVDSGTQF 298
            + +  TPL   P  D F Y V L GI V G ++  +  S+   D  G G  ++DSGT  
Sbjct: 326 RIARF-TPLLSNPKLDTF-YYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSV 385

Query: 299 TFLLGPVYTALKNEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLML 358
           T L+ P Y A+++ F V  K +      P+F      D+CF + +N  ++  +PTV L  
Sbjct: 386 TRLIRPAYIAMRDAFRVGAKTL---KRAPDF---SLFDTCFDL-SNMNEV-KVPTVVLHF 445

Query: 359 NGAEMVVGEELLLYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAK 418
            GA+  V      Y +P    G    +C  F  + + G++  +IGN  QQ   + YDLA 
Sbjct: 446 RGAD--VSLPATNYLIPVDTNG---KFCFAFAGT-MGGLS--IIGNIQQQGFRVVYDLAS 484

[Close]

BLAST of Carg06021 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.5e-29
Identity = 116/380 (30.53%), Postives = 172/380 (45.26%), Query Frame = 0


Query: 51  HNVTLTVELTVGSPPQAVTMVLDTGSELPWLNCK--KKTQTLTSVFNPLSSSSYSRIPCI 110
           H     V   +G+PPQ + MVLDT ++  WL C         ++ FN  SSS+YS + C 
Sbjct: 100 HIGNYVVRAKLGTPPQLMFMVLDTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCS 159

Query: 111 SPICQKQTRDLPNPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCM- 170
           +  C  Q R L  P    +  +C+   SY   SS   +L  DT  +     P   FGC+ 
Sbjct: 160 TAQC-TQARGLTCPSSSPQPSVCSFNQSYGGDSSFSASLVQDTLTLAPDVIPNFSFGCIN 219

Query: 171 -GLGSSSNPQEDAKTTGLMGMNRGSLSFVTQ---LGLSKFSYCISGRDS---SGVLILGE 230
              G+S  PQ      GLMG+ RG +S V+Q   L    FSYC+    S   SG L LG 
Sbjct: 220 SASGNSLPPQ------GLMGLGRGPMSLVSQTTSLYSGVFSYCLPSFRSFYFSGSLKLGL 279

Query: 231 GNHSWVGNMAYTPLVQMSTPLPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQT 290
                  ++ YTPL++ +   PS     Y V L G+ VG+  + ++   L  D      T
Sbjct: 280 LGQP--KSIRYTPLLR-NPRRPS----LYYVNLTGVSVGSVQVPVDPVYLTFDANSGAGT 339

Query: 291 MVDSGTQFTFLLGPVYTALKNEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPP 350
           ++DSGT  T    PVY A+++EF  Q       +   +F   GA D+CF   A+   + P
Sbjct: 340 IIDSGTVITRFAQPVYEAIRDEFRKQ-------VNVSSFSTLGAFDTCF--SADNENVAP 399

Query: 351 LPTVSLMLNGAEMVVGEELLLYRVPGMVKGGDWVYCMTF-GNSDLLGIAAFVIGNHHQQN 410
             T+ +     ++ + E  L++   G +       C++  G          VI N  QQN
Sbjct: 400 KITLHMTSLDLKLPM-ENTLIHSSAGTLT------CLSMAGIRQNANAVLNVIANLQQQN 449

Query: 411 LWMEYDLAKSRLGFVETRCD 420
           L + +D+  SR+G     C+
Sbjct: 460 LRILFDVPNSRIGIAPEPCN 449

[Close]

BLAST of Carg06021 vs. ExPASy TrEMBL
Match: A0A6J1GUY9 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111457415 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 1.7e-246
Identity = 424/429 (98.83%), Postives = 427/429 (99.53%), Query Frame = 0


Query: 1   MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELT 60
           MNFFLHLLQLLVFLLCFKQSLCF ATQAMVLPLKTETG+NSQPSNKLSFHHNVTLTVELT
Sbjct: 1   MNFFLHLLQLLVFLLCFKQSLCFCATQAMVLPLKTETGLNSQPSNKLSFHHNVTLTVELT 60

Query: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120
           VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP
Sbjct: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120

Query: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180
           NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK
Sbjct: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240

Query: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK
Sbjct: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300

Query: 301 NEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELL 360
           NEFLVQTKGVLVPLGDPNFVFQGA+DSCFRVPAN+GKLPPLPTVSLMLNGAEMVVG ELL
Sbjct: 301 NEFLVQTKGVLVPLGDPNFVFQGAMDSCFRVPANQGKLPPLPTVSLMLNGAEMVVGGELL 360

Query: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420
           LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS
Sbjct: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420

Query: 421 AGQRLGLDL 430
           AGQRLGLDL
Sbjct: 421 AGQRLGLDL 429

[Close]

BLAST of Carg06021 vs. ExPASy TrEMBL
Match: A0A6J1JV61 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111487788 PE=3 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 2.7e-239
Identity = 415/429 (96.74%), Postives = 419/429 (97.67%), Query Frame = 0


Query: 1   MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELT 60
           M+FFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETG+NSQPSNKLSFHHNVTLTVELT
Sbjct: 1   MDFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGLNSQPSNKLSFHHNVTLTVELT 60

Query: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120
           VGSPPQAVTMVLDTGSELPWLNC KKTQTLTSVFNPLSSSSYSRIPC SPICQKQTRDLP
Sbjct: 61  VGSPPQAVTMVLDTGSELPWLNC-KKTQTLTSVFNPLSSSSYSRIPCSSPICQKQTRDLP 120

Query: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180
           NPVVCD EKLCNVFVSYADGSSLEGNLASDTFRIGS NKPGTLFGCMG  SSSNPQEDAK
Sbjct: 121 NPVVCDSEKLCNVFVSYADGSSLEGNLASDTFRIGSFNKPGTLFGCMGSDSSSNPQEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240

Query: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK
Sbjct: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300

Query: 301 NEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELL 360
           NEFLVQTKGVLVPL DPNFVFQGA+DSCFRVPAN+GKLPPLP VSLMLNGAEMVVG ELL
Sbjct: 301 NEFLVQTKGVLVPLDDPNFVFQGAMDSCFRVPANQGKLPPLPAVSLMLNGAEMVVGGELL 360

Query: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420
           LYRVPGMVKGGDWVYCMTFGNSDLLG AAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS
Sbjct: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGTAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420

Query: 421 AGQRLGLDL 430
           AGQRLGLDL
Sbjct: 421 AGQRLGLDL 428

[Close]

BLAST of Carg06021 vs. ExPASy TrEMBL
Match: A0A6J1FDS6 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111444820 PE=3 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 2.5e-197
Identity = 338/427 (79.16%), Postives = 377/427 (88.29%), Query Frame = 0


Query: 1   MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELT 60
           M FFL LLQLL+  + FKQ LCFSATQ MVLPLKT+ GV S+PSNKLSFHHNVTLTV LT
Sbjct: 1   MAFFLRLLQLLICCVSFKQGLCFSATQTMVLPLKTQMGVTSRPSNKLSFHHNVTLTVSLT 60

Query: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120
           +GSPPQ VTMVLDTGSEL WL+C KKT  L SVFNPLSSSSYS +PC SP+C+ +TRDLP
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHC-KKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLP 120

Query: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180
           NPV CD +KLC+VFVSYAD SSLEGNLASDTFR+GS  +PGT FGCM  G SSN +EDAK
Sbjct: 121 NPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGL KFSYCISGRDSSGVL+ G+ + SW+GN+ YTPLVQMSTP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTP 240

Query: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LP YDR AYTV+L+GI+VGNKILAL KSI  PDHTGAGQTMVDSGTQFTFLLGPVYTALK
Sbjct: 241 LPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300

Query: 301 NEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELL 360
           NEF+VQTKG+LVPLGDPNFVFQGA+D C+RVP  +GKLPPLP VSLM  GAEMVVG E+L
Sbjct: 301 NEFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVL 360

Query: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420
           +Y+VPGMV+GGD V+C+TFGNSDLLGI AFVIG+HHQQN+WME+DL KSR+GFVETRCD 
Sbjct: 361 MYKVPGMVRGGDQVHCLTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDL 420

Query: 421 AGQRLGL 428
           AG+RLGL
Sbjct: 421 AGERLGL 426

[Close]

BLAST of Carg06021 vs. ExPASy TrEMBL
Match: A0A6J1JZR1 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111488906 PE=3 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 3.3e-197
Identity = 339/427 (79.39%), Postives = 376/427 (88.06%), Query Frame = 0


Query: 1   MNFFLHLLQLLVFLLCFKQSLCFSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELT 60
           M FFL LL LL+  + FKQSLCFSA Q MVLPLKT+ GV SQPSNKLSFHHNVTLTV LT
Sbjct: 1   MAFFLRLLHLLICCVSFKQSLCFSAIQTMVLPLKTQMGVTSQPSNKLSFHHNVTLTVSLT 60

Query: 61  VGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLP 120
           +GSPPQ VTMVLDTGSEL WL+C KKT  L SVFNPLSSSSYS +PC SP+C+ +TRDLP
Sbjct: 61  LGSPPQPVTMVLDTGSELSWLHC-KKTPNLNSVFNPLSSSSYSPVPCASPVCRTRTRDLP 120

Query: 121 NPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAK 180
           NPV CD +KLC+VFVSYAD SSLEGNLASDTFR+GS  +PGT FGCM  G SSN +EDAK
Sbjct: 121 NPVTCDPKKLCHVFVSYADASSLEGNLASDTFRVGSSAQPGTFFGCMDSGFSSNSEEDAK 180

Query: 181 TTGLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTP 240
           TTGLMGMNRGSLSFVTQLGL KFSYCISGRDSSGVL+ G+ + SW+GN+ YTPLVQMSTP
Sbjct: 181 TTGLMGMNRGSLSFVTQLGLPKFSYCISGRDSSGVLLFGDASLSWLGNLTYTPLVQMSTP 240

Query: 241 LPSYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300
           LP YDR AYTV+L+GI+VGNKILAL KSI  PDHTGAGQTMVDSGTQFTFLLGPVYTALK
Sbjct: 241 LPYYDRVAYTVQLDGIRVGNKILALPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALK 300

Query: 301 NEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELL 360
           NEF+VQTKG+LVPLGDPNFVFQGA+D C+RVP  +GKLPPLP VSLM  GAEMVVG E+L
Sbjct: 301 NEFVVQTKGILVPLGDPNFVFQGAMDLCYRVPEKQGKLPPLPVVSLMFRGAEMVVGGEVL 360

Query: 361 LYRVPGMVKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDS 420
           +YRVPGMV+GGD V+C+TFGNSDLLGI AFVIG+HHQQN+WME+DL KSR+GFVETRCD 
Sbjct: 361 MYRVPGMVRGGDQVHCVTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDL 420

Query: 421 AGQRLGL 428
           AG+RLGL
Sbjct: 421 AGERLGL 426

[Close]

BLAST of Carg06021 vs. ExPASy TrEMBL
Match: A0A5D3DLY6 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00670 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.4e-187
Identity = 327/413 (79.18%), Postives = 360/413 (87.17%), Query Frame = 0


Query: 18  KQSLCFSAT-QAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELTVGSPPQAVTMVLDTGS 77
           KQSLCFSAT   MVLPL+T+ G+ SQPSNKLSFHHNVTLTV LTVGSPPQ VTMVLDTGS
Sbjct: 2   KQSLCFSATPTTMVLPLQTQMGLISQPSNKLSFHHNVTLTVSLTVGSPPQQVTMVLDTGS 61

Query: 78  ELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLPNPVVCDEEKLCNVFVS 137
           EL WL+C KK+  LTSVFNPLSSSSYS IPC SP+C+ +TRDLPNPV CD +KLC+  VS
Sbjct: 62  ELSWLHC-KKSPNLTSVFNPLSSSSYSPIPCSSPVCRTRTRDLPNPVTCDPKKLCHAIVS 121

Query: 138 YADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAKTTGLMGMNRGSLSFVT 197
           YAD SSLEGNLASD FRIGS   PGTLFGCM  G SSN +EDAKTTGLMGMNRGSLSFVT
Sbjct: 122 YADASSLEGNLASDNFRIGSSALPGTLFGCMDSGFSSNSEEDAKTTGLMGMNRGSLSFVT 181

Query: 198 QLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTPLPSYDRFAYTVKLEGI 257
           QLGL KFSYCISGRDSSGVL+ G+ + SW+GN+ YTPLVQ+STPLP +DR AYTV+L+GI
Sbjct: 182 QLGLPKFSYCISGRDSSGVLLFGDSHLSWLGNLTYTPLVQISTPLPYFDRVAYTVQLDGI 241

Query: 258 KVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGVLVPLGD 317
           +VGNKIL L KSI  PDHTGAGQTMVDSGTQFTFLLGPVYTAL+NEFL QTKGVL PLGD
Sbjct: 242 RVGNKILPLPKSIFAPDHTGAGQTMVDSGTQFTFLLGPVYTALRNEFLEQTKGVLAPLGD 301

Query: 318 PNFVFQGALDSCFRVPANKGKLPPLPTVSLMLNGAEMVVGEELLLYRVPGMVKGGDWVYC 377
           PNFVFQGA+D C+RVPA  GKLP LP VSLM  GAEMVVG E+LLY+VPGM+KG +WVYC
Sbjct: 302 PNFVFQGAMDLCYRVPAG-GKLPELPAVSLMFRGAEMVVGGEVLLYKVPGMMKGKEWVYC 361

Query: 378 MTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDSAGQRLGLDL 430
           +TFGNSDLLGI AFVIG+HHQQN+WME+DL KSR+GFVETRCD AGQRLGL L
Sbjct: 362 LTFGNSDLLGIEAFVIGHHHQQNVWMEFDLVKSRVGFVETRCDLAGQRLGLGL 412

[Close]

BLAST of Carg06021 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 531.6 bits (1368), Expect = 6.0e-151
Identity = 274/428 (64.02%), Postives = 329/428 (76.87%), Query Frame = 0


Query: 6   HLLQLLVFLLCFKQSLC--FSATQAMVLPLKTETGVNSQPSNKLSFHHNVTLTVELTVGS 65
           + L++ V LL F  + C   S  Q ++  LKT+  +    S+KLSF HNVTLTV L VG 
Sbjct: 15  NFLRISVLLLIFPLTFCKTSSTNQTLLFSLKTQK-LPQSSSDKLSFRHNVTLTVTLAVGD 74

Query: 66  PPQAVTMVLDTGSELPWLNCKKKTQTLTSVFNPLSSSSYSRIPCISPICQKQTRDLPNPV 125
           PPQ ++MVLDTGSEL WL+C KK+  L SVFNP+SSS+YS +PC SPIC+ +TRDLP P 
Sbjct: 75  PPQNISMVLDTGSELSWLHC-KKSPNLGSVFNPVSSSTYSPVPCSSPICRTRTRDLPIPA 134

Query: 126 VCD-EEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSSSNPQEDAKTT 185
            CD +  LC+V +SYAD +S+EGNLA +TF IGS+ +PGTLFGCM  G SSN +EDAK+T
Sbjct: 135 SCDPKTHLCHVAISYADATSIEGNLAHETFVIGSVTRPGTLFGCMDSGLSSNSEEDAKST 194

Query: 186 GLMGMNRGSLSFVTQLGLSKFSYCISGRDSSGVLILGEGNHSWVGNMAYTPLVQMSTPLP 245
           GLMGMNRGSLSFV QLG SKFSYCISG DSSG L+LG+ ++SW+G + YTPLV  STPLP
Sbjct: 195 GLMGMNRGSLSFVNQLGFSKFSYCISGSDSSGFLLLGDASYSWLGPIQYTPLVLQSTPLP 254

Query: 246 SYDRFAYTVKLEGIKVGNKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALKNE 305
            +DR AYTV+LEGI+VG+KIL+L KS+ VPDHTGAGQTMVDSGTQFTFL+GPVYTALKNE
Sbjct: 255 YFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFTFLMGPVYTALKNE 314

Query: 306 FLVQTKGVLVPLGDPNFVFQGALDSCFRV-PANKGKLPPLPTVSLMLNGAEMVVGEELLL 365
           F+ QTK VL  + DP+FVFQG +D C++V    +     LP VSLM  GAEM V  + LL
Sbjct: 315 FITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMFRGAEMSVSGQKLL 374

Query: 366 YRVPGM-VKGGDWVYCMTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFV-ETRCD 425
           YRV G   +G + VYC TFGNSDLLGI AFVIG+HHQQN+WME+DLAKSR+GF    RCD
Sbjct: 375 YRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLAKSRVGFAGNVRCD 434

Query: 426 SAGQRLGL 428
            A QRLGL
Sbjct: 435 LASQRLGL 440

[Close]

BLAST of Carg06021 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 493.8 bits (1270), Expect = 1.4e-139
Identity = 241/411 (58.64%), Postives = 305/411 (74.21%), Query Frame = 0


Query: 24  SATQAMVLPLKTE-TGVNSQPSNKLSFHHNVTLTVELTVGSPPQAVTMVLDTGSELPWLN 83
           S++Q +VLPLKT  T  + +P++KL FHHNVTLTV LTVG+PPQ ++MV+DTGSEL WL 
Sbjct: 41  SSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELSWLR 100

Query: 84  CKKKTQ-TLTSVFNPLSSSSYSRIPCISPICQKQTRDLPNPVVCDEEKLCNVFVSYADGS 143
           C + +     + F+P  SSSYS IPC SP C+ +TRD   P  CD +KLC+  +SYAD S
Sbjct: 101 CNRSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSYADAS 160

Query: 144 SLEGNLASDTFRIG-SLNKPGTLFGCMGLGSSSNPQEDAKTTGLMGMNRGSLSFVTQLGL 203
           S EGNLA++ F  G S N    +FGCMG  S S+P+ED KTTGL+GMNRGSLSF++Q+G 
Sbjct: 161 SSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFISQMGF 220

Query: 204 SKFSYCISGRDS-SGVLILGEGNHSWVGNMAYTPLVQMSTPLPSYDRFAYTVKLEGIKVG 263
            KFSYCISG D   G L+LG+ N +W+  + YTPL+++STPLP +DR AYTV+L GIKV 
Sbjct: 221 PKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTGIKVN 280

Query: 264 NKILALEKSILVPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGVLVPLGDPNF 323
            K+L + KS+LVPDHTGAGQTMVDSGTQFTFLLGPVYTAL++ FL +T G+L    DP+F
Sbjct: 281 GKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYEDPDF 340

Query: 324 VFQGALDSCFR---VPANKGKLPPLPTVSLMLNGAEMVVGEELLLYRVPGMVKGGDWVYC 383
           VFQG +D C+R   V    G L  LPTVSL+  GAE+ V  + LLYRVP +  G D VYC
Sbjct: 341 VFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGNDSVYC 400

Query: 384 MTFGNSDLLGIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRCDSAGQRLGL 428
            TFGNSDL+G+ A+VIG+HHQQN+W+E+DL +SR+G     CD +GQRLG+
Sbjct: 401 FTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGI 451

[Close]

BLAST of Carg06021 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.8e-62
Identity = 143/393 (36.39%), Postives = 208/393 (52.93%), Query Frame = 0


Query: 40  NSQPSN-KLSFHHNVTLTVELTVGSPPQAVTMVLDTGSELPWLNC--KKKTQTLTSVFNP 99
           +S P N +  F +++ L + L +G+PPQA  MVLDTGS+L W+ C  KK      + F+P
Sbjct: 56  SSPPYNFRSRFKYSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPPKPKTSFDP 115

Query: 100 LSSSSYSRIPCISPICQKQTRDLPNPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGS 159
             SSS+S +PC  P+C+ +  D   P  CD  +LC+    YADG+  EGNL  +     +
Sbjct: 116 SLSSSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSN 175

Query: 160 LN-KPGTLFGCMGLGSSSNPQEDAKTTGLMGMNRGSLSFVTQLGLSKFSYCI------SG 219
               P  + GC          E +   G++GMNRG LSFV+Q  +SKFSYCI       G
Sbjct: 176 TEITPPLILGC--------ATESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPG 235

Query: 220 RDSSGVLILGEGNHSWVGNMAYTPLVQM--STPLPSYDRFAYTVKLEGIKVGNKILALEK 279
              +G   LG+  +S      Y  L+    S  +P+ D  AYTV + GI+ G K L +  
Sbjct: 236 FTPTGSFYLGDNPNS--HGFKYVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISG 295

Query: 280 SILVPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGVLVPLGDPNFVFQGALDS 339
           S+  PD  G+GQTMVDSG++FT L+   Y  ++ E + +    L       +V+ G  D 
Sbjct: 296 SVFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRL----KKGYVYGGTADM 355

Query: 340 CFRVPANKGKLPPL--PTVSLMLNGAEMVVGEELLLYRVPGMVKGGDWVYCMTFGNSDLL 399
           CF    N   +P L    V +   G E++V +E +L  V      G  ++C+  G S +L
Sbjct: 356 CF--DGNVAMIPRLIGDLVFVFTRGVEILVPKERVLVNV------GGGIHCVGIGRSSML 415

Query: 400 GIAAFVIGNHHQQNLWMEYDLAKSRLGFVETRC 419
           G A+ +IGN HQQNLW+E+D+   R+GF +  C
Sbjct: 416 GAASNIIGNVHQQNLWVEFDVTNRRVGFAKADC 426

[Close]

BLAST of Carg06021 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 232.6 bits (592), Expect = 5.8e-61
Identity = 155/450 (34.44%), Postives = 230/450 (51.11%), Query Frame = 0


Query: 4   FLHLLQLLVFLLCFKQSLCFSATQAMVLPLKT------------ETGVNSQ-----PSNK 63
           FL LL +  F  C+  SL +S++ ++  PL +            +T + S+     PS+ 
Sbjct: 9   FLKLLYIF-FFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSSP 68

Query: 64  LSFHHNV----TLTVELTVGSPPQAVTMVLDTGSELPWLNC-----KKKTQTLTSVFNPL 123
            +F  N+     L + L +G+P Q+  +VLDTGS+L W+ C     KK     T+ F+P 
Sbjct: 69  YTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPTTSFDPS 128

Query: 124 SSSSYSRIPCISPICQKQTRDLPNPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRI-GS 183
            SSS+S +PC  P+C+ +  D   P  CD  +LC+    YADG+  EGNL  + F    S
Sbjct: 129 LSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSNS 188

Query: 184 LNKPGTLFGCMGLGSSSNPQEDAKTTGLMGMNRGSLSFVTQLGLSKFSYCISGRD----- 243
              P  + GC         +E     G++GMN G LSF++Q  +SKFSYCI  R      
Sbjct: 189 QTTPPLILGC--------AKESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNRPGL 248

Query: 244 -SSGVLILGEGNHSWVGNMAYTPLVQMSTPLPSYDRFAYTVKLEGIKVGNKILALEKSIL 303
            S+G   LG+  +S             S  +P+ D  AYTV L+GI++G K L +  S+ 
Sbjct: 249 ASTGSFYLGDNPNSRGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKRLNIPGSVF 308

Query: 304 VPDHTGAGQTMVDSGTQFTFLLGPVYTALKNEFLVQTKGVLVPLGDPNFVFQGALDSCF- 363
            PD  G+GQTMVDSG++FT L+   Y  +K E +V+  G  +  G   +V+    D CF 
Sbjct: 309 RPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEE-IVRLVGSRLKKG---YVYGSTADMCFD 368

Query: 364 -RVPANKGKLPPLPTVSLMLNGAEMVVGEELLLYRVPGMVKGGDWVYCMTFGNSDLLGIA 419
                  G+L     V     G E++V ++ LL  V      G  ++C+  G S +LG A
Sbjct: 369 GNHSMEIGRLIG-DLVFEFGRGVEILVEKQSLLVNV------GGGIHCVGIGRSSMLGAA 428

[Close]

BLAST of Carg06021 vs. TAIR 10
Match: AT3G54400.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 140.2 bits (352), Expect = 3.9e-33
Identity = 121/377 (32.10%), Postives = 176/377 (46.68%), Query Frame = 0


Query: 54  TLTVELTVGSPPQAVTMVLDTGSELPWLNCKKKTQTLTSV-FNPLSSSSYSRIPCISPIC 113
           T  V   +G+P Q + + LDT ++  W+ C       +SV F+P  SSS   + C +P C
Sbjct: 87  TYIVRANIGTPAQPMLVALDTSNDAAWIPCSGCVGCSSSVLFDPSKSSSSRTLQCEAPQC 146

Query: 114 QKQTRDLPNPVVCDEEKLCNVFVSYADGSSLEGNLASDTFRIGSLNKPGTLFGCMGLGSS 173
               +  PNP  C   K C   ++Y  GS++E  L  DT  + S   P   FGC+   S 
Sbjct: 147 ----KQAPNP-SCTVSKSCGFNMTYG-GSTIEAYLTQDTLTLASDVIPNYTFGCINKASG 206

Query: 174 SNPQEDAKTTGLMGMNRGSLSFVTQ---LGLSKFSYCISGRDS---SGVLILGEGNHSWV 233
           ++        GLMG+ RG LS ++Q   L  S FSYC+    S   SG L LG  N    
Sbjct: 207 TS----LPAQGLMGLGRGPLSLISQSQNLYQSTFSYCLPNSKSSNFSGSLRLGPKNQ--- 266

Query: 234 GNMAYTPLVQMSTPLPSYDRFA--YTVKLEGIKVGNKILALEKSILVPD-HTGAGQTMVD 293
                 P+   +TPL    R +  Y V L GI+VGNKI+ +  S L  D  TGAG T+ D
Sbjct: 267 ------PIRIKTTPLLKNPRRSSLYYVNLVGIRVGNKIVDIPTSALAFDPATGAG-TIFD 326

Query: 294 SGTQFTFLLGPVYTALKNEFLVQTKGVLVPLGDPNFVFQGALDSCFRVPANKGKLPPLPT 353
           SGT +T L+ P Y A++NEF  + K       + N    G  D+C+      G +   P+
Sbjct: 327 SGTVYTRLVEPAYVAVRNEFRRRVK-------NANATSLGGFDTCY-----SGSV-VFPS 386

Query: 354 VSLMLNGAEMVV-GEELLLYRVPGMVKGGDWVYCMTFGNSDL-LGIAAFVIGNHHQQNLW 413
           V+ M  G  + +  + LL++   G +       C+    + + +     VI +  QQN  
Sbjct: 387 VTFMFAGMNVTLPPDNLLIHSSAGNLS------CLAMAAAPVNVNSVLNVIASMQQQNHR 424

Query: 414 MEYDLAKSRLGFVETRC 419
           +  D+  SRLG     C
Sbjct: 447 VLIDVPNSRLGISRETC 424

The following BLAST results are available for this feature:

BLAST of Carg06021 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033342.1	1.3e-248	100.00	Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022955370.1	3.5e-246	98.83	aspartic proteinase PCS1-like [Cucurbita moschata]	[more]
XP_023540416.1	6.3e-243	97.43	aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6602656.1	5.9e-241	98.11	Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022991088.1	5.5e-239	96.74	aspartic proteinase PCS1-like [Cucurbita maxima]	[more]

BLAST of Carg06021 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZL3	1.9e-138	58.64	Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1	[more]
Q766C2	1.4e-43	31.89	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q766C3	3.4e-42	29.92	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q9LNJ3	2.6e-34	32.97	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
O04496	2.5e-29	30.53	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]

BLAST of Carg06021 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GUY9	1.7e-246	98.83	aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111457415 PE=3...	[more]
A0A6J1JV61	2.7e-239	96.74	aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111487788 PE=3 S...	[more]
A0A6J1FDS6	2.5e-197	79.16	aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111444820 PE=3...	[more]
A0A6J1JZR1	3.3e-197	79.39	aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111488906 PE=3 S...	[more]
A0A5D3DLY6	1.4e-187	79.18	Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]

BLAST of Carg06021 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39710.1	6.0e-151	64.02	Eukaryotic aspartyl protease family protein	[more]
AT5G02190.1	1.4e-139	58.64	Eukaryotic aspartyl protease family protein	[more]
AT1G66180.1	1.8e-62	36.39	Eukaryotic aspartyl protease family protein	[more]
AT5G37540.1	5.8e-61	34.44	Eukaryotic aspartyl protease family protein	[more]
AT3G54400.1	3.9e-33	32.10	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 48..219 e-value: 2.5E-34 score: 120.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 221..422 e-value: 1.4E-39 score: 137.6
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 55..419
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 57..219 e-value: 1.3E-37 score: 129.8
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 248..414 e-value: 9.1E-33 score: 113.3
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47965	ASPARTYL PROTEASE-RELATED	coord: 6..427
None	No IPR available	PANTHER	PTHR47965:SF49	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 6..427
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 55..414 score: 34.993565
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 54..418 e-value: 1.9258E-69 score: 219.057

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06021-RA	Carg06021-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity