Carg04441 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04441
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	50S ribosomal protein L12, chloroplastic-like
Location	Carg_Chr04: 20393674 .. 20394249 (+)
RNA-Seq Expression	Carg04441
Synteny	Carg04441

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCACACTCTCCACTCTCACCACCCTCCGTTCCCCACCGCACCTTTCTTATTCCCATTCCTCTTTTTCCACTCAGTCCTCCATTTCCACAACCCTCCAATTTCCCTTTAACCCTTCCTCCACCGCCACCTCCCATCGCGCCACCTACCTCCGTCCAATTGCCGCCGTGTCAGCCCCTGAGAAAATCGAAAAGCTCGGCGCCGACATCTCCAACCTCACTCTCGAAGAAGCCCGCATTCTGGTCGACTTTCTTCAAGATAAGCTTGGGGTCTCCGCTGCCGCATTCGCCCCCGCCGCCGTCGTCGCAGCACCCGGCGCCGCAGCCGGGGACGCGGCCGCGGAGGCGGTGGAGGAGAAGACGGAATTCGATGTGGTCATTGAGGACGTCCCAAGCAATGCTAGAATTGCAGTGATTAAGGCAGTGAGGGCATTGACAAGCTTGGCTTTGAAGGAAGCCAAAGAACTCATCGAAGGTTTGCCCAAGAAGTTCAAGGAAGGGATTTCGAAAGAAGAGGCTGAGGATGCCAAGAAACAGCTTGAAGAAGCTGGAGCTAAAATCGCCATTGTTTAA

mRNA sequence

ATGGCTTCCACACTCTCCACTCTCACCACCCTCCGTTCCCCACCGCACCTTTCTTATTCCCATTCCTCTTTTTCCACTCAGTCCTCCATTTCCACAACCCTCCAATTTCCCTTTAACCCTTCCTCCACCGCCACCTCCCATCGCGCCACCTACCTCCGTCCAATTGCCGCCGTGTCAGCCCCTGAGAAAATCGAAAAGCTCGGCGCCGACATCTCCAACCTCACTCTCGAAGAAGCCCGCATTCTGGTCGACTTTCTTCAAGATAAGCTTGGGGTCTCCGCTGCCGCATTCGCCCCCGCCGCCGTCGTCGCAGCACCCGGCGCCGCAGCCGGGGACGCGGCCGCGGAGGCGGTGGAGGAGAAGACGGAATTCGATGTGGTCATTGAGGACGTCCCAAGCAATGCTAGAATTGCAGTGATTAAGGCAGTGAGGGCATTGACAAGCTTGGCTTTGAAGGAAGCCAAAGAACTCATCGAAGGTTTGCCCAAGAAGTTCAAGGAAGGGATTTCGAAAGAAGAGGCTGAGGATGCCAAGAAACAGCTTGAAGAAGCTGGAGCTAAAATCGCCATTGTTTAA

Coding sequence (CDS)

ATGGCTTCCACACTCTCCACTCTCACCACCCTCCGTTCCCCACCGCACCTTTCTTATTCCCATTCCTCTTTTTCCACTCAGTCCTCCATTTCCACAACCCTCCAATTTCCCTTTAACCCTTCCTCCACCGCCACCTCCCATCGCGCCACCTACCTCCGTCCAATTGCCGCCGTGTCAGCCCCTGAGAAAATCGAAAAGCTCGGCGCCGACATCTCCAACCTCACTCTCGAAGAAGCCCGCATTCTGGTCGACTTTCTTCAAGATAAGCTTGGGGTCTCCGCTGCCGCATTCGCCCCCGCCGCCGTCGTCGCAGCACCCGGCGCCGCAGCCGGGGACGCGGCCGCGGAGGCGGTGGAGGAGAAGACGGAATTCGATGTGGTCATTGAGGACGTCCCAAGCAATGCTAGAATTGCAGTGATTAAGGCAGTGAGGGCATTGACAAGCTTGGCTTTGAAGGAAGCCAAAGAACTCATCGAAGGTTTGCCCAAGAAGTTCAAGGAAGGGATTTCGAAAGAAGAGGCTGAGGATGCCAAGAAACAGCTTGAAGAAGCTGGAGCTAAAATCGCCATTGTTTAA

Protein sequence

MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSAPEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQLEEAGAKIAIV

Homology

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BLAST of Carg04441 vs. NCBI nr
Match: KAG7033112.1 (50S ribosomal protein L12-1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 333.2 bits (853), Expect = 1.5e-87
Identity = 191/191 (100.00%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA 60
           MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA
Sbjct: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA 60

Query: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE 120
           PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE
Sbjct: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 191

[Close]

BLAST of Carg04441 vs. NCBI nr
Match: XP_022960313.1 (50S ribosomal protein L12, chloroplastic-like [Cucurbita moschata] >KAG6602432.1 50S ribosomal protein L12-1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 331.3 bits (848), Expect = 5.7e-87
Identity = 190/191 (99.48%), Postives = 190/191 (99.48%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA 60
           MASTLSTLTTLRSPPHLSYSHSSFSTQSS STTLQFPFNPSSTATSHRATYLRPIAAVSA
Sbjct: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSTSTTLQFPFNPSSTATSHRATYLRPIAAVSA 60

Query: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE 120
           PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE
Sbjct: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 191

[Close]

BLAST of Carg04441 vs. NCBI nr
Match: XP_023536532.1 (50S ribosomal protein L12, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330.1 bits (845), Expect = 1.3e-86
Identity = 190/191 (99.48%), Postives = 190/191 (99.48%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA 60
           MASTLSTLTTLRSP HLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA
Sbjct: 1   MASTLSTLTTLRSPTHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA 60

Query: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE 120
           PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE
Sbjct: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 191

[Close]

BLAST of Carg04441 vs. NCBI nr
Match: XP_022989905.1 (50S ribosomal protein L12, chloroplastic [Cucurbita maxima])

HSP 1 Score: 323.2 bits (827), Expect = 1.5e-84
Identity = 187/191 (97.91%), Postives = 188/191 (98.43%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA 60
           MASTLSTLT+LRSP HLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRAT LRPIAAVSA
Sbjct: 1   MASTLSTLTSLRSPLHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATLLRPIAAVSA 60

Query: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE 120
           PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPA VVAAPGAAAGDAAAEAVEE
Sbjct: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAVVVAAPGAAAGDAAAEAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 191

[Close]

BLAST of Carg04441 vs. NCBI nr
Match: XP_038890576.1 (50S ribosomal protein L12, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 282.3 bits (721), Expect = 3.0e-72
Identity = 169/192 (88.02%), Postives = 177/192 (92.19%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA 60
           MASTLS L TL SP HLSYSHSS ST S  STTLQFP + SS A SHRAT+LRPIAA+SA
Sbjct: 1   MASTLSIL-TLHSPSHLSYSHSSLSTHSFKSTTLQFPSHLSSIAVSHRATHLRPIAAISA 60

Query: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAP-GAAAGDAAAEAVE 120
           PEKIEKLG+DISNLTLEEAR+LVDFLQDKLGVSAA+FAPAAVVAAP G A G+AAAEAVE
Sbjct: 61  PEKIEKLGSDISNLTLEEARLLVDFLQDKLGVSAASFAPAAVVAAPGGTAGGEAAAEAVE 120

Query: 121 EKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKK 180
           EKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEG+SKEEAEDAKK
Sbjct: 121 EKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGVSKEEAEDAKK 180

Query: 181 QLEEAGAKIAIV 192
           QLEEAGAKIAIV
Sbjct: 181 QLEEAGAKIAIV 191

[Close]

BLAST of Carg04441 vs. ExPASy Swiss-Prot
Match: P36688 (50S ribosomal protein L12, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 1.2e-52
Identity = 134/193 (69.43%), Postives = 158/193 (81.87%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSI---STTLQFPFNPSSTATSHRATYLRPIAA 60
           MASTLST+ TLRSP       ++ ST +SI     TL+FP   +    + RAT+LRP+AA
Sbjct: 1   MASTLSTI-TLRSPS----PSTATSTHASIPFPKKTLEFPIR-TPKLQNRRATFLRPLAA 60

Query: 61  VSAPEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEA 120
           V APEK+ +LG +ISNLTL +A+ LV++LQDKLGV+AA+FAPAAVVAAPGAAA   A   
Sbjct: 61  VEAPEKVVQLGDEISNLTLADAQKLVEYLQDKLGVTAASFAPAAVVAAPGAAA--EAPAV 120

Query: 121 VEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDA 180
           VEEKTEFDVVI++VPSNARIA IKAVRALTSLALKEAKELIEGLPKKFKEG+SK+EAEDA
Sbjct: 121 VEEKTEFDVVIDEVPSNARIATIKAVRALTSLALKEAKELIEGLPKKFKEGVSKDEAEDA 180

Query: 181 KKQLEEAGAKIAI 191
           KKQLEEAGAK++I
Sbjct: 181 KKQLEEAGAKVSI 185

[Close]

BLAST of Carg04441 vs. ExPASy Swiss-Prot
Match: P24929 (50S ribosomal protein L12, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL12 PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 4.7e-52
Identity = 135/196 (68.88%), Postives = 155/196 (79.08%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFN----PSSTATSH--RATYLRP 60
           MASTLST+ TLRSP        S ST SS   ++ FP      P  T   H  RAT+LRP
Sbjct: 1   MASTLSTI-TLRSP--------SPSTASSTHASIPFPKKALEFPIRTPKLHHRRATFLRP 60

Query: 61  IAAVSAPEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAA 120
           +AAV APEK+ +LG +ISNLTL +A+ LV++LQDKLGV+AA+FAPAAV AAPGAAA   A
Sbjct: 61  LAAVEAPEKVVQLGDEISNLTLADAQKLVEYLQDKLGVTAASFAPAAVAAAPGAAA--EA 120

Query: 121 AEAVEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEA 180
              VEEKTEFDVVI++VPSNARIA IKAVRALTSLALKEAKELIEGLPKKFKEG+SK+EA
Sbjct: 121 PAVVEEKTEFDVVIDEVPSNARIATIKAVRALTSLALKEAKELIEGLPKKFKEGVSKDEA 180

Query: 181 EDAKKQLEEAGAKIAI 191
           EDAKKQLEEAGAK++I
Sbjct: 181 EDAKKQLEEAGAKVSI 185

[Close]

BLAST of Carg04441 vs. ExPASy Swiss-Prot
Match: P02398 (50S ribosomal protein L12, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL12 PE=1 SV=2)

HSP 1 Score: 204.9 bits (520), Expect = 8.0e-52
Identity = 132/194 (68.04%), Postives = 154/194 (79.38%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSS---TATSHRATYLRPIAA 60
           MA+T +T+ TL  P     SH + ST       LQFPF  ++   + +S R T LRPIAA
Sbjct: 1   MAAT-TTMATLNLPS--LTSHPNSSTFPKHPQPLQFPFRTTTNPISLSSTRTTRLRPIAA 60

Query: 61  VSAPEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEA 120
           V APEKIE+LG  +S LTLEEAR+LVD+LQDKLGVSAA+FAPAA VAAPGA A   AA A
Sbjct: 61  VEAPEKIEQLGTQLSGLTLEEARVLVDWLQDKLGVSAASFAPAAAVAAPGAPAD--AAPA 120

Query: 121 VEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDA 180
           VEEKTEFDV I++VPSNARI+VIKAVRALTSL LKEAKELIEGLPKK KEG+SK++AEDA
Sbjct: 121 VEEKTEFDVSIDEVPSNARISVIKAVRALTSLGLKEAKELIEGLPKKLKEGVSKDDAEDA 180

Query: 181 KKQLEEAGAKIAIV 192
           KKQLE+AGAK++IV
Sbjct: 181 KKQLEDAGAKVSIV 189

[Close]

BLAST of Carg04441 vs. ExPASy Swiss-Prot
Match: P36210 (50S ribosomal protein L12-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL12A PE=2 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 5.8e-50
Identity = 129/198 (65.15%), Postives = 155/198 (78.28%), Query Frame = 0


Query: 2   ASTLSTLTTLRSPPHLSYSHSSFSTQSSI------STTLQFPF---NPSSTATSHRATYL 61
           ++TLS  TT+RS        SS+ T +SI      +TT++FP      SS+  +HRAT+L
Sbjct: 3   STTLSIATTIRS--------SSYPTLASINHFPSRTTTIEFPSRFGGGSSSTLTHRATHL 62

Query: 62  RPIAAVSAPEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGD 121
           RPIAAV APEKIEK+G++IS+LTLEEARILVD+LQDK GVS  + APAA  AA  A A  
Sbjct: 63  RPIAAVEAPEKIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAA--AAVAAPADG 122

Query: 122 AAAEAVEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKE 181
            AA  VEE+TEFDVVI +VPS++RIAVIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+
Sbjct: 123 GAAAVVEEQTEFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKD 182

Query: 182 EAEDAKKQLEEAGAKIAI 191
           EAE+AKK LEEAGAK++I
Sbjct: 183 EAEEAKKTLEEAGAKVSI 190

[Close]

BLAST of Carg04441 vs. ExPASy Swiss-Prot
Match: P36212 (50S ribosomal protein L12-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL12C PE=2 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 1.7e-49
Identity = 128/191 (67.02%), Postives = 153/191 (80.10%), Query Frame = 0


Query: 2   ASTLSTLTTLRSPPHLSYSHSSFSTQSSIS--TTLQFPFNPSSTATSHRATYLRPIAAVS 61
           ++TLS  TT+RS   L    +S ST   +S  T ++FPF  SS ++SHRA  LRPI+AV 
Sbjct: 3   STTLSIATTIRSSSPL----TSASTHHFLSKPTAIEFPFRLSS-SSSHRAINLRPISAVE 62

Query: 62  APEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVE 121
           APEKIEK+G++IS+LTLEEARILVD+LQDK GVS  + APAA  AA  A A   AA  VE
Sbjct: 63  APEKIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAA--AAVAAPADGGAAAVVE 122

Query: 122 EKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKK 181
           E+TEFDVVI +VPS++RIAVIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+EAE+AKK
Sbjct: 123 EQTEFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKDEAEEAKK 182

Query: 182 QLEEAGAKIAI 191
            LEEAGAK++I
Sbjct: 183 TLEEAGAKVSI 186

[Close]

BLAST of Carg04441 vs. ExPASy TrEMBL
Match: A0A6J1H8H9 (50S ribosomal protein L12, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111461079 PE=3 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 2.7e-87
Identity = 190/191 (99.48%), Postives = 190/191 (99.48%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA 60
           MASTLSTLTTLRSPPHLSYSHSSFSTQSS STTLQFPFNPSSTATSHRATYLRPIAAVSA
Sbjct: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSTSTTLQFPFNPSSTATSHRATYLRPIAAVSA 60

Query: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE 120
           PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE
Sbjct: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 191

[Close]

BLAST of Carg04441 vs. ExPASy TrEMBL
Match: A0A6J1JNN0 (50S ribosomal protein L12, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111486956 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 7.5e-85
Identity = 187/191 (97.91%), Postives = 188/191 (98.43%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA 60
           MASTLSTLT+LRSP HLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRAT LRPIAAVSA
Sbjct: 1   MASTLSTLTSLRSPLHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATLLRPIAAVSA 60

Query: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVEE 120
           PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPA VVAAPGAAAGDAAAEAVEE
Sbjct: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAVVVAAPGAAAGDAAAEAVEE 120

Query: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180
           KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ
Sbjct: 121 KTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKKQ 180

Query: 181 LEEAGAKIAIV 192
           LEEAGAKIAIV
Sbjct: 181 LEEAGAKIAIV 191

[Close]

BLAST of Carg04441 vs. ExPASy TrEMBL
Match: A0A6J1BXR2 (50S ribosomal protein L12, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111006430 PE=3 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.8e-70
Identity = 171/193 (88.60%), Postives = 178/193 (92.23%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTAT-SHRATYLRPIAAVS 60
           MASTLSTL TLRSP +LS+S  S ST SS  + +QFPF PSS A  SHRAT LRPIAAVS
Sbjct: 1   MASTLSTL-TLRSPSYLSHSPRSISTHSS-KSAVQFPFYPSSAAAISHRATLLRPIAAVS 60

Query: 61  APEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAA-AGDAAAEAV 120
           APEKIEKLG+DISNLTLEEAR+LVDFLQDKLGVSAAAFAPAAVVAAPGAA AGDAAAEAV
Sbjct: 61  APEKIEKLGSDISNLTLEEARLLVDFLQDKLGVSAAAFAPAAVVAAPGAAGAGDAAAEAV 120

Query: 121 EEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK 180
           EEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEG+SKEEAEDAK
Sbjct: 121 EEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGVSKEEAEDAK 180

Query: 181 KQLEEAGAKIAIV 192
           KQLEEAGAKIAIV
Sbjct: 181 KQLEEAGAKIAIV 191

[Close]

BLAST of Carg04441 vs. ExPASy TrEMBL
Match: A0A6J1JTI3 (50S ribosomal protein L12, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111489595 PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.4e-67
Identity = 164/192 (85.42%), Postives = 171/192 (89.06%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA 60
           MASTLSTL TLR+P  LS+SHSSFST SS  TTL FP        SHRAT LRPIAAVSA
Sbjct: 1   MASTLSTL-TLRTPSLLSHSHSSFSTLSSKPTTLPFP--------SHRATRLRPIAAVSA 60

Query: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAP-GAAAGDAAAEAVE 120
           PEKIEKLG+DISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAP G A GD AAEAVE
Sbjct: 61  PEKIEKLGSDISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGGVAGGDVAAEAVE 120

Query: 121 EKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKK 180
           EKTEFDVVIE+VPSNARIAVIK+VRALT+LALKEAKELIEGLPKKFKEG+SKEEAEDAKK
Sbjct: 121 EKTEFDVVIEEVPSNARIAVIKSVRALTNLALKEAKELIEGLPKKFKEGVSKEEAEDAKK 180

Query: 181 QLEEAGAKIAIV 192
           QLEEAGAKI IV
Sbjct: 181 QLEEAGAKITIV 183

[Close]

BLAST of Carg04441 vs. ExPASy TrEMBL
Match: A0A6J1FMH9 (50S ribosomal protein L12, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445547 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.6e-66
Identity = 163/192 (84.90%), Postives = 170/192 (88.54%), Query Frame = 0


Query: 1   MASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFNPSSTATSHRATYLRPIAAVSA 60
           MASTLSTL TLR+P  LS+SHSSFST SS  TTL F         SHR T LRPIAAVSA
Sbjct: 1   MASTLSTL-TLRTPSLLSHSHSSFSTLSSKPTTLPF--------LSHRTTRLRPIAAVSA 60

Query: 61  PEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAP-GAAAGDAAAEAVE 120
           PEKIEKLG+DISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAP G A GDAAAEAVE
Sbjct: 61  PEKIEKLGSDISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGGVAGGDAAAEAVE 120

Query: 121 EKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKK 180
           EKTEFDVVIE+VPSNARIAVIK+VRALT+LALKEAKELIEGLPKKFKEG+SKEEAEDAKK
Sbjct: 121 EKTEFDVVIEEVPSNARIAVIKSVRALTNLALKEAKELIEGLPKKFKEGVSKEEAEDAKK 180

Query: 181 QLEEAGAKIAIV 192
           QLEEAGAKI IV
Sbjct: 181 QLEEAGAKITIV 183

[Close]

BLAST of Carg04441 vs. TAIR 10
Match: AT3G27830.1 (ribosomal protein L12-A )

HSP 1 Score: 198.7 bits (504), Expect = 4.1e-51
Identity = 129/198 (65.15%), Postives = 155/198 (78.28%), Query Frame = 0


Query: 2   ASTLSTLTTLRSPPHLSYSHSSFSTQSSI------STTLQFPF---NPSSTATSHRATYL 61
           ++TLS  TT+RS        SS+ T +SI      +TT++FP      SS+  +HRAT+L
Sbjct: 3   STTLSIATTIRS--------SSYPTLASINHFPSRTTTIEFPSRFGGGSSSTLTHRATHL 62

Query: 62  RPIAAVSAPEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGD 121
           RPIAAV APEKIEK+G++IS+LTLEEARILVD+LQDK GVS  + APAA  AA  A A  
Sbjct: 63  RPIAAVEAPEKIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAA--AAVAAPADG 122

Query: 122 AAAEAVEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKE 181
            AA  VEE+TEFDVVI +VPS++RIAVIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+
Sbjct: 123 GAAAVVEEQTEFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKD 182

Query: 182 EAEDAKKQLEEAGAKIAI 191
           EAE+AKK LEEAGAK++I
Sbjct: 183 EAEEAKKTLEEAGAKVSI 190

[Close]

BLAST of Carg04441 vs. TAIR 10
Match: AT3G27850.1 (ribosomal protein L12-C )

HSP 1 Score: 197.2 bits (500), Expect = 1.2e-50
Identity = 128/191 (67.02%), Postives = 153/191 (80.10%), Query Frame = 0


Query: 2   ASTLSTLTTLRSPPHLSYSHSSFSTQSSIS--TTLQFPFNPSSTATSHRATYLRPIAAVS 61
           ++TLS  TT+RS   L    +S ST   +S  T ++FPF  SS ++SHRA  LRPI+AV 
Sbjct: 3   STTLSIATTIRSSSPL----TSASTHHFLSKPTAIEFPFRLSS-SSSHRAINLRPISAVE 62

Query: 62  APEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAVE 121
           APEKIEK+G++IS+LTLEEARILVD+LQDK GVS  + APAA  AA  A A   AA  VE
Sbjct: 63  APEKIEKIGSEISSLTLEEARILVDYLQDKFGVSPLSLAPAA--AAVAAPADGGAAAVVE 122

Query: 122 EKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAKK 181
           E+TEFDVVI +VPS++RIAVIKAVRALTSLALKEAKELIEGLPKKFKEGI+K+EAE+AKK
Sbjct: 123 EQTEFDVVINEVPSSSRIAVIKAVRALTSLALKEAKELIEGLPKKFKEGITKDEAEEAKK 182

Query: 182 QLEEAGAKIAI 191
            LEEAGAK++I
Sbjct: 183 TLEEAGAKVSI 186

[Close]

BLAST of Carg04441 vs. TAIR 10
Match: AT3G27840.1 (ribosomal protein L12-B )

HSP 1 Score: 167.2 bits (422), Expect = 1.3e-41
Identity = 109/193 (56.48%), Postives = 139/193 (72.02%), Query Frame = 0


Query: 2   ASTLSTLTTLRSPPHLSYSHSSFSTQSSISTTLQFPFN---PSSTATSHRATYLRPIAAV 61
           A+TLS  TT+RS    S   S+    S    +++FPF+    SS+  SHRA YL PI+AV
Sbjct: 3   ATTLSIATTIRSSSFSSGLASAHHFPSR-PLSIEFPFSFGVSSSSTLSHRAIYLHPISAV 62

Query: 62  SAPEKIEKLGADISNLTLEEARILVDFLQDKLGVSAAAFAPAAVVAAPGAAAGDAAAEAV 121
             P+KI+K+G++IS+LTLEE+RILVD++QDK GVS    APAA    P    G A A +V
Sbjct: 63  KTPKKIKKIGSEISSLTLEESRILVDYVQDKFGVSILFSAPAAAALPPPLDNGGATA-SV 122

Query: 122 EEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEAEDAK 181
           E +T FDVVI DVP   RIAVI A+RA+TSL+L E+KELIEG PKKFKEG++K+EAE+ K
Sbjct: 123 ERQTTFDVVINDVPRGNRIAVITAIRAMTSLSLSESKELIEGFPKKFKEGVTKDEAEEDK 182

Query: 182 KQLEEAGAKIAIV 192
            QLEEAGAK++IV
Sbjct: 183 TQLEEAGAKVSIV 193

[Close]

BLAST of Carg04441 vs. TAIR 10
Match: AT4G36420.1 (Ribosomal protein L12 family protein )

HSP 1 Score: 78.2 bits (191), Expect = 8.1e-15
Identity = 55/136 (40.44%), Postives = 82/136 (60.29%), Query Frame = 0


Query: 62  EKIEKLGADISNLTLEEARILVDFLQDKLGVS----AAAFAPAAVVAAPGA---AAGDAA 121
           E + K+  ++SNLTL E   L + L+ KL +S     AA  P   +   GA   A G+  
Sbjct: 43  ENVSKIVNELSNLTLLETMDLTEILRQKLNISELPVMAAMMPGMSLPGSGASKSAGGEGK 102

Query: 122 AEAVEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALKEAKELIEGLPKKFKEGISKEEA 181
            +  E KT FDV+++   + ++I VIK VR +T+L LKEAK+L+E  P   K+G+SKEEA
Sbjct: 103 EKKKEAKTAFDVMLQAYDAVSKIKVIKEVRTITALGLKEAKDLVEKAPTLLKKGVSKEEA 162

Query: 182 EDAKKQLEEAGAKIAI 191
           E   ++L+  GAK+A+
Sbjct: 163 EKIIEKLKAVGAKVAM 178

[Close]

BLAST of Carg04441 vs. TAIR 10
Match: AT4G37660.1 (Ribosomal protein L12/ ATP-dependent Clp protease adaptor protein ClpS family protein )

HSP 1 Score: 62.8 bits (151), Expect = 3.5e-10
Identity = 56/158 (35.44%), Postives = 82/158 (51.90%), Query Frame = 0


Query: 40  PSSTATSHRATYLRPIAAVSAPE----KIEKLGADISNLTLEEARILVDFLQDKLGVSAA 99
           P S+AT H       + AV++PE    K+E++  D+  L   E          K+G++  
Sbjct: 21  PLSSATRH-------LCAVASPEARTKKLERIADDLLKLNRIELYDYSLLFSHKIGLNRY 80

Query: 100 AFAPAAVVAAPGAAAGDAAAEA---VEEKTEFDVVIEDVPSNARIAVIKAVRALTSLALK 159
             A A        + GDA+A       EKT FDV +E   + ++I VIK +RA T L LK
Sbjct: 81  GSAVAVT-----GSVGDASASTETKTAEKTTFDVKLEKFEAASKIKVIKEIRAFTDLGLK 140

Query: 160 EAKELIEGLPKKFKEGISKEEAEDAKKQLEEAGAKIAI 191
           EAK L+E  P   K G++KEEAE   ++L+  GA +A+
Sbjct: 141 EAKALVEKAPVIVKNGLTKEEAEKIMEKLKAVGAIVAL 166

The following BLAST results are available for this feature:

BLAST of Carg04441 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033112.1	1.5e-87	100.00	50S ribosomal protein L12-1, chloroplastic, partial [Cucurbita argyrosperma subs...	[more]
XP_022960313.1	5.7e-87	99.48	50S ribosomal protein L12, chloroplastic-like [Cucurbita moschata] >KAG6602432.1...	[more]
XP_023536532.1	1.3e-86	99.48	50S ribosomal protein L12, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022989905.1	1.5e-84	97.91	50S ribosomal protein L12, chloroplastic [Cucurbita maxima]	[more]
XP_038890576.1	3.0e-72	88.02	50S ribosomal protein L12, chloroplastic-like [Benincasa hispida]	[more]

BLAST of Carg04441 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P36688	1.2e-52	69.43	50S ribosomal protein L12, chloroplastic OS=Nicotiana sylvestris OX=4096 PE=2 SV...	[more]
P24929	4.7e-52	68.88	50S ribosomal protein L12, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL12 P...	[more]
P02398	8.0e-52	68.04	50S ribosomal protein L12, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL12 P...	[more]
P36210	5.8e-50	65.15	50S ribosomal protein L12-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RP...	[more]
P36212	1.7e-49	67.02	50S ribosomal protein L12-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RP...	[more]

BLAST of Carg04441 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H8H9	2.7e-87	99.48	50S ribosomal protein L12, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1JNN0	7.5e-85	97.91	50S ribosomal protein L12, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A6J1BXR2	1.8e-70	88.60	50S ribosomal protein L12, chloroplastic-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1JTI3	1.4e-67	85.42	50S ribosomal protein L12, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A6J1FMH9	1.6e-66	84.90	50S ribosomal protein L12, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of Carg04441 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G27830.1	4.1e-51	65.15	ribosomal protein L12-A	[more]
AT3G27850.1	1.2e-50	67.02	ribosomal protein L12-C	[more]
AT3G27840.1	1.3e-41	56.48	ribosomal protein L12-B	[more]
AT4G36420.1	8.1e-15	40.44	Ribosomal protein L12 family protein	[more]
AT4G37660.1	3.5e-10	35.44	Ribosomal protein L12/ ATP-dependent Clp protease adaptor protein ClpS family pr...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013823	Ribosomal protein L7/L12, C-terminal	PFAM	PF00542	Ribosomal_L12	coord: 124..190 e-value: 4.1E-24 score: 84.7
IPR000206	Ribosomal protein L7/L12	TIGRFAM	TIGR00855	TIGR00855	coord: 63..190 e-value: 9.3E-33 score: 111.3
IPR000206	Ribosomal protein L7/L12	PANTHER	PTHR45987	39S RIBOSOMAL PROTEIN L12	coord: 1..190
IPR000206	Ribosomal protein L7/L12	HAMAP	MF_00368	Ribosomal_L7_L12	coord: 59..191 score: 22.636374
IPR000206	Ribosomal protein L7/L12	CDD	cd00387	Ribosomal_L7_L12	coord: 62..190 e-value: 2.83413E-40 score: 131.509
IPR036235	Ribosomal protein L7/L12, oligomerisation domain superfamily	GENE3D	1.20.5.710	Single helix bin	coord: 63..119 e-value: 1.6E-13 score: 52.5
IPR036235	Ribosomal protein L7/L12, oligomerisation domain superfamily	SUPERFAMILY	48300	Ribosomal protein L7/12, oligomerisation (N-terminal) domain	coord: 63..126
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	GENE3D	3.30.1390.10		coord: 120..190 e-value: 9.1E-26 score: 91.8
IPR014719	Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like	SUPERFAMILY	54736	ClpS-like	coord: 119..190
IPR008932	Ribosomal protein L7/L12, oligomerisation	PFAM	PF16320	Ribosomal_L12_N	coord: 63..112 e-value: 2.2E-13 score: 49.7
None	No IPR available	PANTHER	PTHR45987:SF16	50S RIBOSOMAL PROTEIN L12-1, CHLOROPLASTIC-RELATED	coord: 1..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04441-RA	Carg04441-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0005840	ribosome
molecular_function	GO:0003735	structural constituent of ribosome