Carg23644 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg23644
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionBOI-related E3 ubiquitin-protein ligase 1-like
LocationCarg_Chr03: 2784706 .. 2786032 (-)
RNA-Seq ExpressionCarg23644
SyntenyCarg23644
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTTCAAGCTCAATATCCTTCAAATGTTTTGCTCTTAAACAGGTATACCCCTTCTCTTTTGCCTCTAATCTCTATTTCTCTACTTACTGTTGATTCCTAATGGTTTTGGATTTTGGATTTTGGATTTTGGATTTTGGATTTTGTTTTAGAAATGGTCAAGAAGCGCATGATTATTCATCGCAGATACAACCAGGAGGTTTTCTTGACCAGACTCATATGGTGTTCAACAACAATATAGGTGAGATTTTTGTGCATTGTGTTTGATCTATTCTGTTTATAAACGCTAAAAGTATTTCTATCAATTGAGTTGTGAAGACGACGTACCTTTGAGGATTCGATTCATCAGTCCGGTTTGTGATGAAAGCAGGAAACAATTCTCGGAAGAGAGGAAGAGAAATGGCTACTTCCACAGCAACCTTCGCGACTCCAATCGATCTCTCTGCGATACAGCAGCCTCGTCCTCCTCTTCTTGTTGATCTCGCCAAGCTTCACAATCACCAAAACAATGTCGTCTCCACCGGTCTCCGCTTGTCCTCCGGTAATCATCAACAGAATCAACCGCTGCAACAGCGACAACAACAACAACAACAACCTAATCATAACCACAATCTCGTTCCTCAATCATCATCAACTTTCATATCGCTATTAACAGACGATTTCGCCTCGAATTTCAAACGGCAACAAGACGAAATAGATCAATTCCTTCGAGCACAGGTGCTAATTCACTCTCCCTAACACAGGAAACGAGTTTCATCTTTCGTCTTGATTTTTTCTTTTTTTTCTCAATGAACTCCGGTTTCATTTCAAAACAGGAAGAGCAACTACGGCTCACCTTGGCAGATAAAAGACAGAGACACTACCGTGCACTCCTGGATGCAGTAGAGGAATCGGTAGCGAGAAGGTTAAGAGAGAGAGAGGCGGAGGTTGAGAAAGCCACGCGCCGAAACGCCGAGTTGGAAGCACGCGCCGCCCAGCTTAGTGTCGAGGCGCAGGTCTGGCAAGCGAAGGCTAGGGCACAGGAAGCCACCGCTGTAGCTCTCCAGGCACAACTGCAACAGGCGATGTCTGGGGGATGCGACGGAGGAGACGGCGGCACGGCCGGCGCCGAAGGACAAACCGACGACGCCGAGTCGGCTCATATCGACCCGGACCGAGTGAGGATTTTGGGACCGAGTTGCAAAGCGTGTAGAAAGCGGTTGGCGTCGGTGTTGCTTTTGCCCTGCCGTCATCTCTGCCTCTGTACAGACTGCGACCAAGTGGCTCAGACTTGCCCGTTATGCCCTTCCAAGCGAAGTTCCAGCGTAGAGGTCTATCTCTCCTAA

mRNA sequence

ATGGCTGTTCAAGCTCAATATCCTTCAAATGTTTTGCTCTTAAACAGAAATGGTCAAGAAGCGCATGATTATTCATCGCAGATACAACCAGGAGGTTTTCTTGACCAGACTCATATGGTGTTCAACAACAATATAGTCCGGTTTGTGATGAAAGCAGGAAACAATTCTCGGAAGAGAGGAAGAGAAATGGCTACTTCCACAGCAACCTTCGCGACTCCAATCGATCTCTCTGCGATACAGCAGCCTCGTCCTCCTCTTCTTGTTGATCTCGCCAAGCTTCACAATCACCAAAACAATGTCGTCTCCACCGGTCTCCGCTTGTCCTCCGGTAATCATCAACAGAATCAACCGCTGCAACAGCGACAACAACAACAACAACAACCTAATCATAACCACAATCTCGTTCCTCAATCATCATCAACTTTCATATCGCTATTAACAGACGATTTCGCCTCGAATTTCAAACGGCAACAAGACGAAATAGATCAATTCCTTCGAGCACAGGAAGAGCAACTACGGCTCACCTTGGCAGATAAAAGACAGAGACACTACCGTGCACTCCTGGATGCAGTAGAGGAATCGGTAGCGAGAAGGTTAAGAGAGAGAGAGGCGGAGGTTGAGAAAGCCACGCGCCGAAACGCCGAGTTGGAAGCACGCGCCGCCCAGCTTAGTGTCGAGGCGCAGGTCTGGCAAGCGAAGGCTAGGGCACAGGAAGCCACCGCTGTAGCTCTCCAGGCACAACTGCAACAGGCGATGTCTGGGGGATGCGACGGAGGAGACGGCGGCACGGCCGGCGCCGAAGGACAAACCGACGACGCCGAGTCGGCTCATATCGACCCGGACCGAGTGAGGATTTTGGGACCGAGTTGCAAAGCGTGTAGAAAGCGGTTGGCGTCGGTGTTGCTTTTGCCCTGCCGTCATCTCTGCCTCTGTACAGACTGCGACCAAGTGGCTCAGACTTGCCCGTTATGCCCTTCCAAGCGAAGTTCCAGCGTAGAGGTCTATCTCTCCTAA

Coding sequence (CDS)

ATGGCTGTTCAAGCTCAATATCCTTCAAATGTTTTGCTCTTAAACAGAAATGGTCAAGAAGCGCATGATTATTCATCGCAGATACAACCAGGAGGTTTTCTTGACCAGACTCATATGGTGTTCAACAACAATATAGTCCGGTTTGTGATGAAAGCAGGAAACAATTCTCGGAAGAGAGGAAGAGAAATGGCTACTTCCACAGCAACCTTCGCGACTCCAATCGATCTCTCTGCGATACAGCAGCCTCGTCCTCCTCTTCTTGTTGATCTCGCCAAGCTTCACAATCACCAAAACAATGTCGTCTCCACCGGTCTCCGCTTGTCCTCCGGTAATCATCAACAGAATCAACCGCTGCAACAGCGACAACAACAACAACAACAACCTAATCATAACCACAATCTCGTTCCTCAATCATCATCAACTTTCATATCGCTATTAACAGACGATTTCGCCTCGAATTTCAAACGGCAACAAGACGAAATAGATCAATTCCTTCGAGCACAGGAAGAGCAACTACGGCTCACCTTGGCAGATAAAAGACAGAGACACTACCGTGCACTCCTGGATGCAGTAGAGGAATCGGTAGCGAGAAGGTTAAGAGAGAGAGAGGCGGAGGTTGAGAAAGCCACGCGCCGAAACGCCGAGTTGGAAGCACGCGCCGCCCAGCTTAGTGTCGAGGCGCAGGTCTGGCAAGCGAAGGCTAGGGCACAGGAAGCCACCGCTGTAGCTCTCCAGGCACAACTGCAACAGGCGATGTCTGGGGGATGCGACGGAGGAGACGGCGGCACGGCCGGCGCCGAAGGACAAACCGACGACGCCGAGTCGGCTCATATCGACCCGGACCGAGTGAGGATTTTGGGACCGAGTTGCAAAGCGTGTAGAAAGCGGTTGGCGTCGGTGTTGCTTTTGCCCTGCCGTCATCTCTGCCTCTGTACAGACTGCGACCAAGTGGCTCAGACTTGCCCGTTATGCCCTTCCAAGCGAAGTTCCAGCGTAGAGGTCTATCTCTCCTAA

Protein sequence

MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRGREMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQRQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKRQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASVLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS
Homology
BLAST of Carg23644 vs. NCBI nr
Match: KAG7033614.1 (putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 636.3 bits (1640), Expect = 1.5e-178
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60
           MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60

Query: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120
           REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ
Sbjct: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120

Query: 121 RQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKR 180
           RQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKR
Sbjct: 121 RQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKR 180

Query: 181 QRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT 240
           QRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT
Sbjct: 181 QRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT 240

Query: 241 AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV 300
           AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV
Sbjct: 241 AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV 300

Query: 301 LLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           LLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS
Sbjct: 301 LLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 337

BLAST of Carg23644 vs. NCBI nr
Match: KAG6571752.1 (putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 613.2 bits (1580), Expect = 1.3e-171
Identity = 328/337 (97.33%), Postives = 330/337 (97.92%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60
           MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNI       GNNSRKRG
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNI-------GNNSRKRG 60

Query: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120
           REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ
Sbjct: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120

Query: 121 RQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKR 180
           R+QQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFL+AQEEQLRLTLADKR
Sbjct: 121 RKQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADKR 180

Query: 181 QRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT 240
           QRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT
Sbjct: 181 QRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT 240

Query: 241 AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV 300
           AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV
Sbjct: 241 AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV 300

Query: 301 LLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           LLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS
Sbjct: 301 LLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 330

BLAST of Carg23644 vs. NCBI nr
Match: XP_022932986.1 (BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 603.2 bits (1554), Expect = 1.4e-168
Identity = 325/337 (96.44%), Postives = 327/337 (97.03%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60
           MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNI       GNNSRKRG
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNI-------GNNSRKRG 60

Query: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120
           REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQ 
Sbjct: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQ- 120

Query: 121 RQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKR 180
            QQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFL+AQEEQLRLTLADKR
Sbjct: 121 -QQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADKR 180

Query: 181 QRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT 240
           QRHYRALLDAVEESVARRLRERE+EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT
Sbjct: 181 QRHYRALLDAVEESVARRLRERESEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT 240

Query: 241 AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV 300
           AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV
Sbjct: 241 AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV 300

Query: 301 LLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           LLLPCRHLCLCTDCDQVA TCPLCPSKRSSSVEVYLS
Sbjct: 301 LLLPCRHLCLCTDCDQVATTCPLCPSKRSSSVEVYLS 328

BLAST of Carg23644 vs. NCBI nr
Match: XP_023542941.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 598.6 bits (1542), Expect = 3.4e-167
Identity = 323/338 (95.56%), Postives = 326/338 (96.45%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60
           MAVQAQYPSNVLLLNRNGQEAHDYS QIQPGGFLDQTHMVFNNNI       GNNSRKR 
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSLQIQPGGFLDQTHMVFNNNI-------GNNSRKRA 60

Query: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPL-Q 120
           REMATSTATFATPIDLSAIQQPRPPLLVDLA LHNHQNNVVSTGLRLSSGNHQQNQPL Q
Sbjct: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLANLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120

Query: 121 QRQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADK 180
           Q+QQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFL+AQEEQLRLTLADK
Sbjct: 121 QQQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADK 180

Query: 181 RQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEA 240
           RQRHYRALLDAVEESVARRLRERE EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEA
Sbjct: 181 RQRHYRALLDAVEESVARRLREREEEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEA 240

Query: 241 TAVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLAS 300
           TAVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDP+RVRILGPSCKACRKRLAS
Sbjct: 241 TAVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPERVRILGPSCKACRKRLAS 300

Query: 301 VLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           VLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS
Sbjct: 301 VLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 331

BLAST of Carg23644 vs. NCBI nr
Match: XP_022973264.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima])

HSP 1 Score: 587.4 bits (1513), Expect = 7.8e-164
Identity = 319/340 (93.82%), Postives = 325/340 (95.59%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60
           MAVQAQYPSNVLLLNRNGQEAHDYS QIQPGGFLDQTHMVFNNNI       GNNSRKRG
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSLQIQPGGFLDQTHMVFNNNI-------GNNSRKRG 60

Query: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120
           REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ
Sbjct: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120

Query: 121 RQQQQQQP---NHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLA 180
           +QQQQQQ    NHNHNLV QSSS FISLLTDDFASNFKRQQDEIDQFL+AQEEQLRLTLA
Sbjct: 121 QQQQQQQQQQHNHNHNLVRQSSSAFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLA 180

Query: 181 DKRQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQ 240
           DKRQRHYRALLDAVEESVARRLRE+EAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQ
Sbjct: 181 DKRQRHYRALLDAVEESVARRLREKEAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQ 240

Query: 241 EATAVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRL 300
           EATA+ALQAQL+QAMSGGCDGGDGGTAGAEGQTDDAESA+IDPDRVRILGPSCKACRKRL
Sbjct: 241 EATALALQAQLKQAMSGGCDGGDGGTAGAEGQTDDAESAYIDPDRVRILGPSCKACRKRL 300

Query: 301 ASVLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           ASVLLLPCRHLCLCT CDQVAQTCPLCPSKRSSSVEVYLS
Sbjct: 301 ASVLLLPCRHLCLCTVCDQVAQTCPLCPSKRSSSVEVYLS 333

BLAST of Carg23644 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 9.3e-19
Identity = 70/199 (35.18%), Postives = 111/199 (55.78%), Query Frame = 0

Query: 144 SLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKRQRHYRALLDAVEESVARRLRERE 203
           SL+  +  S  ++Q  EID+F+  Q E LR+ L  +++   R L  AV+ ++ ++L+ ++
Sbjct: 96  SLIDAELVSQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKD 155

Query: 204 AEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVALQAQLQQAMSGGCDGGDGGT 263
            E+ +  + N  L+ R   L VE Q+W+  A+  EATA  L++ L+Q ++   D  D   
Sbjct: 156 EEIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLA-QVDDLDAFR 215

Query: 264 AGAEGQTDDAES---AHIDPDRVRILGPSCKACRKRLASVLLLPCRHLCLCTDCDQVA-- 323
                + DDAES   +    D   ++   CK C +  ASVL+LPCRHLCLCT C   A  
Sbjct: 216 RPLVEEADDAESSCGSCDGGDVTAVVNGGCKRCGELTASVLVLPCRHLCLCTVCGSSALL 275

Query: 324 QTCPLCPSKRSSSVEVYLS 338
           +TCP+C    ++SV V +S
Sbjct: 276 RTCPVCDMVMTASVHVNMS 293

BLAST of Carg23644 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 7.9e-18
Identity = 100/357 (28.01%), Postives = 161/357 (45.10%), Query Frame = 0

Query: 28  IQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRGREMATSTATFATPIDLSAIQQPRPPLL 87
           + P        +  NNN +        N+ + G  +    + F+ P   S      PPLL
Sbjct: 10  LSPPQLFSNRELTMNNNTMEPTSGGFCNNNQTGYGV---VSPFSVPNHTSTTTTATPPLL 69

Query: 88  VDLAKLHNHQNNVVSTGLRLSSGNHQQN-----QPLQQRQQQQQQPNHNHNLV--PQSSS 147
                ++   + + +T    + G    +      P ++R +   + N++H L+  P+SSS
Sbjct: 70  ----HVYGGSDTIPTTAGYYADGATNLDCEFFPLPTRKRSRDSSRSNYHHLLLQNPRSSS 129

Query: 148 -----------TFISLLTD--DFASNFKRQQDEIDQFLRA---QEEQLRLTLADKRQRHY 207
                      T  S L    D +S+  +QQ EID+F+     Q E+++  + +KR+R  
Sbjct: 130 CVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRKRQA 189

Query: 208 RALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVAL 267
           R +++A+E+ + +RLR +E E E+  + N  LE R   LS+E Q+W+  A+  EATA  L
Sbjct: 190 RTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEATANHL 249

Query: 268 QAQLQQAMSGGCDGGDGGTAGAE---GQTDDAESA---------------------HIDP 327
           +  L+  ++   D   G  AG E    + DDAES                          
Sbjct: 250 RTNLEHVLAQVKDVSRG--AGLEKNMNEEDDAESCCGSSCGGGGEETVRRRVGLEREAQD 309

Query: 328 DRVRILGPSCKACRKRLASVLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
              R     C+ C +  + VLLLPCRHLCLC  C     TCP+C S +++SV V +S
Sbjct: 310 KAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSPKNASVHVNMS 357

BLAST of Carg23644 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 6.7e-17
Identity = 81/234 (34.62%), Postives = 127/234 (54.27%), Query Frame = 0

Query: 117 PLQQRQQQQQQPNHNHNLVPQSSSTF----ISLLTDDFASNFK-RQQDEIDQFLRAQEEQ 176
           PL  ++Q+  Q + ++  V + S  F     SL+  +  S  + +QQ EID+F+  Q E+
Sbjct: 74  PLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLINVELVSQIQNQQQSEIDRFVAQQTEK 133

Query: 177 LRLTLADKRQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQ 236
           LR+ +  ++Q   R L  AV+  +A++L+E++ E+ +    N  L+ R   L VE Q+W+
Sbjct: 134 LRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNWVLQERVKSLYVENQIWR 193

Query: 237 AKARAQEATAVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESA------HIDPDRVRI 296
             A+  EA A  L+  L Q ++         TA A  + DDAES+          + V  
Sbjct: 194 DIAQTNEANANTLRTNLDQVLA---QLETFPTASAVVE-DDAESSCGSCCGDGGGEAVTA 253

Query: 297 LGPSCKACRKRLASVLLLPCRHLCLCTDC--DQVAQTCPLCPSKRSSSVEVYLS 338
           +G  CK C +R ASVL+LPCRHLCLCT C    + +TCP+C    ++SV V +S
Sbjct: 254 VGGGCKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASVHVNMS 303

BLAST of Carg23644 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.3e-16
Identity = 84/289 (29.07%), Postives = 138/289 (47.75%), Query Frame = 0

Query: 72  TPIDLSAIQQPRPPL-LVDLAKLHNHQNNV-----VSTGLRLSSGNHQQNQPLQQRQQQQ 131
           +P+D    Q  +P +  VD +   N  NNV     VS+  R    + +++  L      Q
Sbjct: 59  SPVDRLVHQSMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKR----SREESVVLNPSAYMQ 118

Query: 132 QQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKRQRHYR 191
            Q N    L+          L  D +SN ++   +ID+ +    E++R+ + +KR+   R
Sbjct: 119 IQKNPTDPLM---------FLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGR 178

Query: 192 ALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVALQ 251
            +++AVE+ + + LR ++ E+    + N  LE +   L VE Q+W+  A++ EAT  AL+
Sbjct: 179 RIVEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALR 238

Query: 252 AQLQQAMSG-----------------GCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGP 311
           + LQQ ++                   C G +      E +   A  A       R+   
Sbjct: 239 SNLQQVLAAVERNRWEEPPTVADDAQSCCGSNDEGDSEEERWKLAGEAQDTKKMCRVGMS 298

Query: 312 SCKACRKRLASVLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
            C++C K  ASVLLLPCRH+CLC+ C     TCP+C S +++S+ V LS
Sbjct: 299 MCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHVNLS 334

BLAST of Carg23644 vs. ExPASy TrEMBL
Match: A0A6J1EXW3 (BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439606 PE=4 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 6.6e-169
Identity = 325/337 (96.44%), Postives = 327/337 (97.03%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60
           MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNI       GNNSRKRG
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNI-------GNNSRKRG 60

Query: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120
           REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQ 
Sbjct: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQ- 120

Query: 121 RQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKR 180
            QQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFL+AQEEQLRLTLADKR
Sbjct: 121 -QQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLADKR 180

Query: 181 QRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT 240
           QRHYRALLDAVEESVARRLRERE+EVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT
Sbjct: 181 QRHYRALLDAVEESVARRLRERESEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT 240

Query: 241 AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV 300
           AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV
Sbjct: 241 AVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASV 300

Query: 301 LLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           LLLPCRHLCLCTDCDQVA TCPLCPSKRSSSVEVYLS
Sbjct: 301 LLLPCRHLCLCTDCDQVATTCPLCPSKRSSSVEVYLS 328

BLAST of Carg23644 vs. ExPASy TrEMBL
Match: A0A6J1IE25 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111471820 PE=4 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 3.8e-164
Identity = 319/340 (93.82%), Postives = 325/340 (95.59%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60
           MAVQAQYPSNVLLLNRNGQEAHDYS QIQPGGFLDQTHMVFNNNI       GNNSRKRG
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEAHDYSLQIQPGGFLDQTHMVFNNNI-------GNNSRKRG 60

Query: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120
           REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ
Sbjct: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120

Query: 121 RQQQQQQP---NHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLA 180
           +QQQQQQ    NHNHNLV QSSS FISLLTDDFASNFKRQQDEIDQFL+AQEEQLRLTLA
Sbjct: 121 QQQQQQQQQQHNHNHNLVRQSSSAFISLLTDDFASNFKRQQDEIDQFLQAQEEQLRLTLA 180

Query: 181 DKRQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQ 240
           DKRQRHYRALLDAVEESVARRLRE+EAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQ
Sbjct: 181 DKRQRHYRALLDAVEESVARRLREKEAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQ 240

Query: 241 EATAVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRL 300
           EATA+ALQAQL+QAMSGGCDGGDGGTAGAEGQTDDAESA+IDPDRVRILGPSCKACRKRL
Sbjct: 241 EATALALQAQLKQAMSGGCDGGDGGTAGAEGQTDDAESAYIDPDRVRILGPSCKACRKRL 300

Query: 301 ASVLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           ASVLLLPCRHLCLCT CDQVAQTCPLCPSKRSSSVEVYLS
Sbjct: 301 ASVLLLPCRHLCLCTVCDQVAQTCPLCPSKRSSSVEVYLS 333

BLAST of Carg23644 vs. ExPASy TrEMBL
Match: A0A6J1EYB7 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111439606 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 287/299 (95.99%), Postives = 289/299 (96.66%), Query Frame = 0

Query: 39  MVFNNNIVRFVMKAGNNSRKRGREMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQN 98
           MVFNNNI       GNNSRKRGREMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQN
Sbjct: 1   MVFNNNI-------GNNSRKRGREMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQN 60

Query: 99  NVVSTGLRLSSGNHQQNQPLQQRQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQ 158
           NVVSTGLRLSSGNHQQNQPLQ  QQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQ
Sbjct: 61  NVVSTGLRLSSGNHQQNQPLQ--QQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQ 120

Query: 159 DEIDQFLRAQEEQLRLTLADKRQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEA 218
           DEIDQFL+AQEEQLRLTLADKRQRHYRALLDAVEESVARRLRERE+EVEKATRRNAELEA
Sbjct: 121 DEIDQFLQAQEEQLRLTLADKRQRHYRALLDAVEESVARRLRERESEVEKATRRNAELEA 180

Query: 219 RAAQLSVEAQVWQAKARAQEATAVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHI 278
           RAAQLSVEAQVWQAKARAQEATAVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHI
Sbjct: 181 RAAQLSVEAQVWQAKARAQEATAVALQAQLQQAMSGGCDGGDGGTAGAEGQTDDAESAHI 240

Query: 279 DPDRVRILGPSCKACRKRLASVLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           DPDRVRILGPSCKACRKRLASVLLLPCRHLCLCTDCDQVA TCPLCPSKRSSSVEVYLS
Sbjct: 241 DPDRVRILGPSCKACRKRLASVLLLPCRHLCLCTDCDQVATTCPLCPSKRSSSVEVYLS 290

BLAST of Carg23644 vs. ExPASy TrEMBL
Match: A0A1S3CRF0 (BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo OX=3656 GN=LOC103503495 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 289/339 (85.25%), Postives = 307/339 (90.56%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60
           MAVQAQYPSNVLLLNRNGQE HDYS Q QPGGFLDQTHM+FNNNI       G+NSRKRG
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMMFNNNI-------GSNSRKRG 60

Query: 61  REMATSTATFATPIDLSAIQ-QPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQ 120
           RE++T+T T ATPI+LS +Q QP PPLLVDLAKLHNHQNNVVSTGLRLSSG+HQQN    
Sbjct: 61  REISTATTTVATPINLSPLQHQPHPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN---- 120

Query: 121 QRQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADK 180
           Q+QQQQQQ NHNHNLV QSSS F+ LLT+DFASNFKRQQ+EIDQFL+AQEEQLR TLA+K
Sbjct: 121 QQQQQQQQHNHNHNLVRQSSSAFLPLLTEDFASNFKRQQEEIDQFLQAQEEQLRRTLAEK 180

Query: 181 RQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEA 240
           RQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEA
Sbjct: 181 RQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEA 240

Query: 241 TAVALQAQLQQAMS-GGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLA 300
           TAV+LQAQLQQAMS GGCDGGDGGT G EGQ DDAESAHIDP+RV I GPSCKACRKRLA
Sbjct: 241 TAVSLQAQLQQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLA 300

Query: 301 SVLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           S+L+LPCRHLCLC DCDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 SMLILPCRHLCLCADCDQVAQTCPLCHSKRSSSVEVYLS 328

BLAST of Carg23644 vs. ExPASy TrEMBL
Match: A0A0A0LEI4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G911260 PE=4 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.1e-144
Identity = 289/339 (85.25%), Postives = 306/339 (90.27%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60
           MAVQAQYPSNVLLLNRNGQE HDYS Q QPGGFLDQTHMVFNNNI       G+NSRKRG
Sbjct: 1   MAVQAQYPSNVLLLNRNGQEGHDYSLQPQPGGFLDQTHMVFNNNI-------GSNSRKRG 60

Query: 61  REMATSTATFATPIDLSAIQ-QPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQ 120
           RE++T+T T ATPI+LS +Q QP PPLLVDLAKLHNHQNNVVSTGLRLSSG+HQQN    
Sbjct: 61  REISTATTTIATPINLSPLQHQPCPPLLVDLAKLHNHQNNVVSTGLRLSSGDHQQN---- 120

Query: 121 QRQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADK 180
           Q+QQQQQQ N NHNLV  SSS F+ LLT+DFASNFKRQQ+EIDQFL+AQEEQLR TLA+K
Sbjct: 121 QQQQQQQQHNQNHNLVRPSSSAFLPLLTEDFASNFKRQQEEIDQFLQAQEEQLRRTLAEK 180

Query: 181 RQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEA 240
           RQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEA
Sbjct: 181 RQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEA 240

Query: 241 TAVALQAQLQQAMS-GGCDGGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLA 300
           TAV+LQAQLQQAMS GGCDGGDGGT G EGQ DDAESAHIDP+RV I GPSCKACRKRLA
Sbjct: 241 TAVSLQAQLQQAMSGGGCDGGDGGTVGVEGQADDAESAHIDPERVVISGPSCKACRKRLA 300

Query: 301 SVLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           S+L+LPCRHLCLCTDCDQVAQTCPLC SKRSSSVEVYLS
Sbjct: 301 SMLILPCRHLCLCTDCDQVAQTCPLCHSKRSSSVEVYLS 328

BLAST of Carg23644 vs. TAIR 10
Match: AT1G32740.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 223.4 bits (568), Expect = 2.7e-58
Identity = 158/351 (45.01%), Postives = 212/351 (60.40%), Query Frame = 0

Query: 1   MAVQAQ-YPSNVLLLN-RNGQEAHDYSSQIQ---PGGFLDQTHMVFNNNIVRFVMKAGNN 60
           MAVQAQ + SN+L LN RNG+E    +  +Q    G FLDQT+M+FNN          +N
Sbjct: 1   MAVQAQHHSSNLLFLNKRNGKEKEHSNFTLQSQAAGDFLDQTNMLFNN--------GSSN 60

Query: 61  SRKRGREMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQ---NNVVSTGLRLSSGNH 120
            RKR RE             L  +Q  + P ++DL+ LHN+    +N+V TGLRL SG  
Sbjct: 61  QRKRRRETNNH--------QLLPMQSHQFPQVIDLSLLHNYNHPPSNMVHTGLRLFSG-- 120

Query: 121 QQNQPLQQRQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQL 180
                    + Q Q+ +H              L  D FA++  RQ +E+D+FL AQ E+L
Sbjct: 121 ---------EDQAQKISH--------------LSEDVFAAHINRQSEELDEFLHAQAEEL 180

Query: 181 RLTLADKRQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQA 240
           R TLA+KR+ HY+ALL AVEES+ R+LRE+E E+E+ATRR+ EL AR +QL  E QVWQ 
Sbjct: 181 RRTLAEKRKMHYKALLGAVEESLVRKLREKEVEIERATRRHNELVARDSQLRAEVQVWQE 240

Query: 241 KARAQEATAVALQAQLQQAM---SGGCDGGDGGTAGAEG-------QTDDAESAHIDPDR 300
           +A+A E  A +LQ+QLQQA+   +GGC       A  EG         DDAES ++DP+R
Sbjct: 241 RAKAHEDAAASLQSQLQQAVNQCAGGCVSAQDSRAAEEGLLCTTISGVDDAESVYVDPER 300

Query: 301 VRILGPSCKACRKRLASVLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVE 334
           V+   P+CKACR+R A+V++LPCRHL +C  CD+ A  CPLC + R+SSVE
Sbjct: 301 VK--RPNCKACREREATVVVLPCRHLSICPGCDRTALACPLCLTLRNSSVE 308

BLAST of Carg23644 vs. TAIR 10
Match: AT4G17680.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 209.9 bits (533), Expect = 3.1e-54
Identity = 146/343 (42.57%), Postives = 196/343 (57.14%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60
           MA+QAQ+PS     N NGQEA D S Q Q   F + T             KAG +SRKR 
Sbjct: 18  MAIQAQHPSRFFFNNSNGQEASDCSLQPQDTPFTNFT-------------KAGVDSRKRS 77

Query: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120
           RE+ +S      P        P+P  ++D+ +L     NVVSTGLRL    H Q+Q  QQ
Sbjct: 78  REVYSSALMNPPP--------PKPSQVIDITELLQKTPNVVSTGLRLF---HDQSQNQQQ 137

Query: 121 RQQQQQQPNHNHNLVPQSSSTFISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKR 180
                                F S L  D     KRQ+DE+D+F++ Q E+LR TLAD R
Sbjct: 138 ---------------------FFSSLPGDVTGKIKRQRDELDRFIQTQGEELRRTLADNR 197

Query: 181 QRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEAT 240
           +R Y  LL A EE V R+LR++EAE+EKATRR+AELEAR A +  EA+ WQ +A  +EA 
Sbjct: 198 ERRYVELLCAAEEIVGRKLRKKEAELEKATRRHAELEARVAHIVEEARNWQLRAATREAE 257

Query: 241 AVALQAQLQQAMSGGCD--------GGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKA 300
             +L A LQQA++   D        G DGG A    + +DAES ++DP+R+ ++GPSC+ 
Sbjct: 258 VSSLHAHLQQAIANRLDTAAKQSTFGEDGGDA---EEAEDAESVYVDPERIELIGPSCRI 312

Query: 301 CRKRLASVLLLPCRHLCLCTDCDQVA-QTCPLCPSKRSSSVEV 335
           CR++ A+V+ LPC+HL LC  CD  A + CP+C + ++S VEV
Sbjct: 318 CRRKSATVMALPCQHLILCNGCDVGAVRVCPICLAVKTSGVEV 312

BLAST of Carg23644 vs. TAIR 10
Match: AT5G47050.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 208.4 bits (529), Expect = 9.1e-54
Identity = 146/345 (42.32%), Postives = 196/345 (56.81%), Query Frame = 0

Query: 1   MAVQAQYPSNVLLLNRNGQEAHDYSSQIQPGGFLDQTHMVFNNNIVRFVMKAGNNSRKRG 60
           MAVQA +  + L    NGQE +D     QP     Q H +           AG ++RKR 
Sbjct: 1   MAVQAHHHPSTLFFLNNGQEGND-----QP--HKSQFHSI----------NAGVDTRKRA 60

Query: 61  REMATSTATFATPIDLSAIQQPRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQ 120
           RE+++        +D++A     PP             NVVSTGLRLS          +Q
Sbjct: 61  REVSS-----VIDLDITAAPMNPPPQTPPQVIGRRQNPNVVSTGLRLSR---------EQ 120

Query: 121 RQQQQQQPNHNHNLVPQSSSTFISL-LTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADK 180
            Q Q+Q+              F+S  +T D A   K Q DE+++FL+ Q EQL+  LA+ 
Sbjct: 121 SQNQEQR--------------FLSFPITGDVAGEIKSQTDELNRFLQIQGEQLKRMLAEN 180

Query: 181 RQRHYRALLDAVEESVARRLREREAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEA 240
            +R+YR LL   EESV RRLRE+EAE+EKATRR+ ELEARA Q+  EA+ WQ +A A+EA
Sbjct: 181 SERNYRELLRTTEESVRRRLREKEAEIEKATRRHVELEARATQIETEARAWQMRAAAREA 240

Query: 241 TAVALQAQLQQAMSGGCDGG-------DGGTAGAEGQTDDAESAHIDPDRVRILGPSCKA 300
            A +LQAQL QA+     GG         G+     + +DAESA++DPDRV ++GP C+ 
Sbjct: 241 EATSLQAQLHQAVVVAHGGGVITTVEPQSGSVDGVDEAEDAESAYVDPDRVEMIGPGCRI 300

Query: 301 CRKRLASVLLLPCRHLCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           CR+R A+VL LPCRHL +CT+CD   + CPLC S ++SSVEV+ S
Sbjct: 301 CRRRSATVLALPCRHLVMCTECDGSVRICPLCLSTKNSSVEVFYS 300

BLAST of Carg23644 vs. TAIR 10
Match: AT4G17680.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.3e-44
Identity = 116/262 (44.27%), Postives = 160/262 (61.07%), Query Frame = 0

Query: 82  PRPPLLVDLAKLHNHQNNVVSTGLRLSSGNHQQNQPLQQRQQQQQQPNHNHNLVPQSSST 141
           P+P  ++D+ +L     NVVSTGLRL    H Q+Q  QQ                     
Sbjct: 6   PKPSQVIDITELLQKTPNVVSTGLRLF---HDQSQNQQQ--------------------- 65

Query: 142 FISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKRQRHYRALLDAVEESVARRLRE 201
           F S L  D     KRQ+DE+D+F++ Q E+LR TLAD R+R Y  LL A EE V R+LR+
Sbjct: 66  FFSSLPGDVTGKIKRQRDELDRFIQTQGEELRRTLADNRERRYVELLCAAEEIVGRKLRK 125

Query: 202 REAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVALQAQLQQAMSGGCD---- 261
           +EAE+EKATRR+AELEAR A +  EA+ WQ +A  +EA   +L A LQQA++   D    
Sbjct: 126 KEAELEKATRRHAELEARVAHIVEEARNWQLRAATREAEVSSLHAHLQQAIANRLDTAAK 185

Query: 262 ----GGDGGTAGAEGQTDDAESAHIDPDRVRILGPSCKACRKRLASVLLLPCRHLCLCTD 321
               G DGG A    + +DAES ++DP+R+ ++GPSC+ CR++ A+V+ LPC+HL LC  
Sbjct: 186 QSTFGEDGGDA---EEAEDAESVYVDPERIELIGPSCRICRRKSATVMALPCQHLILCNG 240

Query: 322 CDQVA-QTCPLCPSKRSSSVEV 335
           CD  A + CP+C + ++S VEV
Sbjct: 246 CDVGAVRVCPICLAVKTSGVEV 240

BLAST of Carg23644 vs. TAIR 10
Match: AT1G10650.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 118.6 bits (296), Expect = 9.5e-27
Identity = 102/330 (30.91%), Postives = 163/330 (49.39%), Query Frame = 0

Query: 31  GGFLDQTHMVFNNNIVRFVMKAGNNSRKRGREMATSTATFATPIDLSAIQQPRPPLLVDL 90
           G  +D  +   N+N+V  +        KRGRE  + +         + IQ+ +  L + L
Sbjct: 40  GCTVDPVNYFANDNLVPMI-----RPNKRGREAESIS---------NNIQRQQQKLQMSL 99

Query: 91  AKLHNH--------QNNVVSTGLRLSSGNHQQNQPLQQRQQQQQQPNHNHNLVPQSSSTF 150
              +N+        + N+VSTGLRLS  + + N  +                   ++S  
Sbjct: 100 NYNYNNTSVREEVPKENLVSTGLRLSYDDDEHNSSVTSASGS-----------ILAASPI 159

Query: 151 ISLLTDDFASNFKRQQDEIDQFLRAQEEQLRLTLADKRQRHYRALLDAVEESVARRLRER 210
              L D    +  RQ+DE DQF++ Q  Q+   + D +QRH  + L  +E+ V+++L+E+
Sbjct: 160 FQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQRHIASFLTTLEKGVSKKLQEK 219

Query: 211 EAEVEKATRRNAELEARAAQLSVEAQVWQAKARAQEATAVALQAQLQQAMSGG---CDGG 270
           + E+    ++N EL  R  Q+++EAQ W  +A+  E+    L+A LQQAMS         
Sbjct: 220 DHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVNVLKANLQQAMSHNNSVIAAA 279

Query: 271 DGGTAG-AEGQTDDAESAHIDPD-----------RVRILGPSCKACRKRLASVLLLPCRH 330
           D G  G  + + DDA S++IDP+           R+R     CK C  +  SVL++PCRH
Sbjct: 280 DQGKEGFGDSEIDDAASSYIDPNNNNNNNMGIHQRMR-----CKMCNVKEVSVLIVPCRH 339

Query: 331 LCLCTDCDQVAQTCPLCPSKRSSSVEVYLS 338
           L LC +CD   + CP+C S +SS V+V+ S
Sbjct: 340 LSLCKECDVFTKICPVCKSLKSSCVQVFFS 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7033614.11.5e-178100.00putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrospe... [more]
KAG6571752.11.3e-17197.33putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrospe... [more]
XP_022932986.11.4e-16896.44BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 [Cucurbita moschata][more]
XP_023542941.13.4e-16795.56BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022973264.17.8e-16493.82BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FHE49.3e-1935.18BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG... [more]
F4IDI67.9e-1828.01Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37... [more]
O818516.7e-1734.62E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1[more]
Q9LDD13.3e-1629.07Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A6J1EXW36.6e-16996.44BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 OS=Cucurbita moschata ... [more]
A0A6J1IE253.8e-16493.82BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1EYB71.3e-14595.99probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita mosch... [more]
A0A1S3CRF01.3e-14585.25BOI-related E3 ubiquitin-protein ligase 1 OS=Cucumis melo OX=3656 GN=LOC10350349... [more]
A0A0A0LEI41.1e-14485.25RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G911260 P... [more]
Match NameE-valueIdentityDescription
AT1G32740.12.7e-5845.01SBP (S-ribonuclease binding protein) family protein [more]
AT4G17680.13.1e-5442.57SBP (S-ribonuclease binding protein) family protein [more]
AT5G47050.19.1e-5442.32SBP (S-ribonuclease binding protein) family protein [more]
AT4G17680.21.3e-4444.27SBP (S-ribonuclease binding protein) family protein [more]
AT1G10650.19.5e-2730.91SBP (S-ribonuclease binding protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 192..233
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 289..327
e-value: 1.3E-7
score: 31.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 106..136
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 255..277
NoneNo IPR availablePANTHERPTHR42647:SF30BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 1-LIKEcoord: 1..336
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 270..337
e-value: 5.7E-11
score: 43.7
IPR017066E3 ubiquitin-protein ligase BOI-likePIRSFPIRSF036836SBP1_RNase_bindcoord: 1..337
e-value: 3.6E-86
score: 287.8
IPR017066E3 ubiquitin-protein ligase BOI-likePANTHERPTHR42647SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEINcoord: 1..336
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 290..324
score: 9.641949

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg23644-RACarg23644-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity