Cp4.1LG12g07140 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG12g07140
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING-type E3 ubiquitin transferase
LocationCp4.1LG12: 7184900 .. 7185874 (+)
RNA-Seq ExpressionCp4.1LG12g07140
SyntenyCp4.1LG12g07140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTCCGCCTCCGCTGCCACCGCCGCCGCCGCCGAACGGCACACCTACTGGTGCCACGAGTGCGATATGAGCGTCACTTTGGTATCTCCTTCTTCGTCTTCTTCGTCTTCTTCGTCTTCTTCTTCTTCTTCTTCTTCCTCTCTCCTTTGCCCTCACTGCCTCACTGACTTCCTTGAACACATGGATTTCACCATACCCACTTCTTCTTCTTCAATTTCCGACCACCCCATTTCCTCTTCTTCCCCTCCAGATTCCGATCCGTCATCCTTCGTCATCGTCGACCCTCTTCCGACCACCTCCGATGACAATTACCTCCTCAGCAGCCCCCAATTCCTCCGCCTTTTCCAGCAGCTCGCTGATTCATCCGACTCCGATTTCGCACCATCTGTACCCTTCAACCCATTTACTCCGATCAAAGCCTCTGTCATGGCGATTCCCACGATTAAAGTCTCCTCCGCCATGCTCGAGGAAGACCCAATTCTAATTTGTGCTATTTGCAAGGATAAGTTCCTTCTTGAGGTGGAAGCCAAACAGCTCCCCTGTTCTCATCTCTATCATCCTGATTGCATTCTTCCATGGCTTTCTAATCATGATTCGTGCCCTCTTTGCCGCTATAAGCTTCCTTCTGATGAACCTTCGGATCATGTGAGATGTAGAGGAGCAACTATGGCCTTATTGAGGGCTAGGGACCTGATGCCTCAAGAAGATAGTTATGGGTTGAGGACTACTCTTGAACATATGGCTAGAAGGCATAGTTCTATTTTTAGTGAAGGAGTTCAAGTGGATTCGTCTCAATCTCCCACTCAGTTGGGGGTTGCTGTGATGGGAAATGGGGAACAGACTGACAGTGTTGAGACTGTTTCTAGTGTGGCTACTGATGATGGGATTGTAATTGTCAATAGCAGTGGTGATGAAAATGGGTTTGTGGTAATGGATGGACCAATAAATGAGGATGCTGGCACCTTAGCTTAG

mRNA sequence

ATGTCCTCCGCCTCCGCTGCCACCGCCGCCGCCGCCGAACGGCACACCTACTGGTGCCACGAGTGCGATATGAGCGTCACTTTGGTATCTCCTTCTTCGTCTTCTTCGTCTTCTTCGTCTTCTTCTTCTTCTTCTTCTTCCTCTCTCCTTTGCCCTCACTGCCTCACTGACTTCCTTGAACACATGGATTTCACCATACCCACTTCTTCTTCTTCAATTTCCGACCACCCCATTTCCTCTTCTTCCCCTCCAGATTCCGATCCGTCATCCTTCGTCATCGTCGACCCTCTTCCGACCACCTCCGATGACAATTACCTCCTCAGCAGCCCCCAATTCCTCCGCCTTTTCCAGCAGCTCGCTGATTCATCCGACTCCGATTTCGCACCATCTGTACCCTTCAACCCATTTACTCCGATCAAAGCCTCTGTCATGGCGATTCCCACGATTAAAGTCTCCTCCGCCATGCTCGAGGAAGACCCAATTCTAATTTGTGCTATTTGCAAGGATAAGTTCCTTCTTGAGGTGGAAGCCAAACAGCTCCCCTGTTCTCATCTCTATCATCCTGATTGCATTCTTCCATGGCTTTCTAATCATGATTCGTGCCCTCTTTGCCGCTATAAGCTTCCTTCTGATGAACCTTCGGATCATGTGAGATGTAGAGGAGCAACTATGGCCTTATTGAGGGCTAGGGACCTGATGCCTCAAGAAGATAGTTATGGGTTGAGGACTACTCTTGAACATATGGCTAGAAGGCATAGTTCTATTTTTAGTGAAGGAGTTCAAGTGGATTCGTCTCAATCTCCCACTCAGTTGGGGGTTGCTGTGATGGGAAATGGGGAACAGACTGACAGTGTTGAGACTGTTTCTAGTGTGGCTACTGATGATGGGATTGTAATTGTCAATAGCAGTGGTGATGAAAATGGGTTTGTGGTAATGGATGGACCAATAAATGAGGATGCTGGCACCTTAGCTTAG

Coding sequence (CDS)

ATGTCCTCCGCCTCCGCTGCCACCGCCGCCGCCGCCGAACGGCACACCTACTGGTGCCACGAGTGCGATATGAGCGTCACTTTGGTATCTCCTTCTTCGTCTTCTTCGTCTTCTTCGTCTTCTTCTTCTTCTTCTTCTTCCTCTCTCCTTTGCCCTCACTGCCTCACTGACTTCCTTGAACACATGGATTTCACCATACCCACTTCTTCTTCTTCAATTTCCGACCACCCCATTTCCTCTTCTTCCCCTCCAGATTCCGATCCGTCATCCTTCGTCATCGTCGACCCTCTTCCGACCACCTCCGATGACAATTACCTCCTCAGCAGCCCCCAATTCCTCCGCCTTTTCCAGCAGCTCGCTGATTCATCCGACTCCGATTTCGCACCATCTGTACCCTTCAACCCATTTACTCCGATCAAAGCCTCTGTCATGGCGATTCCCACGATTAAAGTCTCCTCCGCCATGCTCGAGGAAGACCCAATTCTAATTTGTGCTATTTGCAAGGATAAGTTCCTTCTTGAGGTGGAAGCCAAACAGCTCCCCTGTTCTCATCTCTATCATCCTGATTGCATTCTTCCATGGCTTTCTAATCATGATTCGTGCCCTCTTTGCCGCTATAAGCTTCCTTCTGATGAACCTTCGGATCATGTGAGATGTAGAGGAGCAACTATGGCCTTATTGAGGGCTAGGGACCTGATGCCTCAAGAAGATAGTTATGGGTTGAGGACTACTCTTGAACATATGGCTAGAAGGCATAGTTCTATTTTTAGTGAAGGAGTTCAAGTGGATTCGTCTCAATCTCCCACTCAGTTGGGGGTTGCTGTGATGGGAAATGGGGAACAGACTGACAGTGTTGAGACTGTTTCTAGTGTGGCTACTGATGATGGGATTGTAATTGTCAATAGCAGTGGTGATGAAAATGGGTTTGTGGTAATGGATGGACCAATAAATGAGGATGCTGGCACCTTAGCTTAG

Protein sequence

MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLEHMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLADSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQLPCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYGLRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIVNSSGDENGFVVMDGPINEDAGTLA
Homology
BLAST of Cp4.1LG12g07140 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.9e-17
Identity = 44/101 (43.56%), Postives = 62/101 (61.39%), Query Frame = 0

Query: 110 PQFLRLFQQLADSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKD 169
           P    L +QL+  +     P  P       K+S+ A+PTIK++   L+      C +CKD
Sbjct: 140 PGLEELIEQLSSGTHHRGPPPAP-------KSSIDALPTIKITQKHLKSSD-SHCPVCKD 199

Query: 170 KFLLEVEAKQLPCSHLYHPDCILPWLSNHDSCPLCRYKLPS 211
           +F L+ EAKQ+PC H+YH DCI+PWL  H+SCP+CR +LPS
Sbjct: 200 EFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPS 232

BLAST of Cp4.1LG12g07140 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 6.4e-17
Identity = 43/101 (42.57%), Postives = 62/101 (61.39%), Query Frame = 0

Query: 110 PQFLRLFQQLADSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKD 169
           P    LF+QL+  +     P  P       ++++ A+PTIK++   L       C +CKD
Sbjct: 144 PGLEELFEQLSAGTTRRGPPPAP-------RSAIDALPTIKIAQRHLRSSD-SNCPVCKD 203

Query: 170 KFLLEVEAKQLPCSHLYHPDCILPWLSNHDSCPLCRYKLPS 211
           +F L  EAKQ+PC+H+YH DCI+PWL  H+SCP+CR +LPS
Sbjct: 204 EFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPS 236

BLAST of Cp4.1LG12g07140 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.0e-14
Identity = 67/207 (32.37%), Postives = 102/207 (49.28%), Query Frame = 0

Query: 28  LVSPSSSSSSSSSSSS----------SSSSSLLCPHCLTDFLEHMDFTIPTSSSSISDHP 87
           L+ PSSSS+++SSS+S           + S    P     F    +      SS      
Sbjct: 94  LLFPSSSSTTTSSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFIQNHLNDLRSSGAQIEF 153

Query: 88  ISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLADSSDSDFAPSVPFNPFT 147
           +  ++P D           LP    D ++   P   +L QQLA++  + +         T
Sbjct: 154 VIQNNPSDQG-------FRLPANIGDYFI--GPGLEQLIQQLAENDPNRYG--------T 213

Query: 148 P--IKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQLPCSHLYHPDCILPWL 207
           P   K+++ A+P + ++ + L  +    CA+C D F    EAKQ+PC HLYH DC+LPWL
Sbjct: 214 PPASKSAIEALPLVNITKSNLNSE-FNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWL 273

Query: 208 SNHDSCPLCRYKLPSDEPSDHVRCRGA 223
             H+SCP+CR++LP+D+P    R RGA
Sbjct: 274 ELHNSCPVCRHELPTDDPDYERRVRGA 282

BLAST of Cp4.1LG12g07140 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.9e-13
Identity = 60/199 (30.15%), Postives = 103/199 (51.76%), Query Frame = 0

Query: 26  VTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLEHMDFTIPTSSSSISDHPISSSSPPD 85
           ++L  PS    + S+  +  S +      L + L+ +  +       I +HP   S P +
Sbjct: 100 MSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNHLQTLRSSGTHFEFVIENHP---SDPGN 159

Query: 86  SDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLADSSDSDFAPSVPFNPFTP--IKASV 145
             P +F            +Y    P   +L QQLA++  + +         TP   K+++
Sbjct: 160 RMPGNF-----------GDYFF-GPGLEQLIQQLAENDPNRYG--------TPPASKSAI 219

Query: 146 MAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQLPCSHLYHPDCILPWLSNHDSCPL 205
            A+PT+KV+  ML+ + +  CA+C D+F    + KQ+PC H++H DC+LPWL  H+SCP+
Sbjct: 220 DALPTVKVTKDMLKSE-MNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPV 274

Query: 206 CRYKLPSDEPSDHVRCRGA 223
           CR++LP+D+P    R +G+
Sbjct: 280 CRFELPTDDPDYENRSQGS 274

BLAST of Cp4.1LG12g07140 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.3e-13
Identity = 70/233 (30.04%), Postives = 122/233 (52.36%), Query Frame = 0

Query: 31  PSSSSSSSSSSSSSSSSSLLCPHCLTDFLEHMDFTIPTSSSSISDHPI----SSSSPPDS 90
           P  SSSSS+ S+S  + +   P  +T            + S+ S +P+     S++ P S
Sbjct: 61  PPPSSSSSTPSASMHADNSPTPTIVT-----------RTRSNRSPNPVIVLRGSAAAPSS 120

Query: 91  -------DPSSFVI---------VDPLPTTSDDNYLLSSPQFLRLFQQLA--DSSDSDFA 150
                  D S+F +         + PLP +  + +LL S  F RL  Q++  + + +   
Sbjct: 121 DVVSEGLDRSAFQMYYDDGTDSGLRPLPPSMTE-FLLGS-GFDRLLDQISQIELNTNRNL 180

Query: 151 PSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQLPCSHLYHP 210
            S    P +  K+++ A+P I++    L  D    CA+CK+ F+L+  A+++PC+H+YHP
Sbjct: 181 RSCEHPPAS--KSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHP 240

Query: 211 DCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYGL 242
           DCILPWL+  +SCP+CR++LP+++ +D     GA +  + A     ++ + GL
Sbjct: 241 DCILPWLAIRNSCPVCRHELPAEDLTDGT---GAALTAVTATAEEEEDSAAGL 275

BLAST of Cp4.1LG12g07140 vs. NCBI nr
Match: XP_023549060.1 (E3 ubiquitin-protein ligase RING1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 616 bits (1589), Expect = 5.53e-222
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60
           MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE
Sbjct: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60

Query: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120
           HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA
Sbjct: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120

Query: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180
           DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL
Sbjct: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180

Query: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240
           PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG
Sbjct: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240

Query: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300
           LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV
Sbjct: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300

Query: 301 NSSGDENGFVVMDGPINEDAGTLA 324
           NSSGDENGFVVMDGPINEDAGTLA
Sbjct: 301 NSSGDENGFVVMDGPINEDAGTLA 324

BLAST of Cp4.1LG12g07140 vs. NCBI nr
Match: XP_022953211.1 (E3 ubiquitin-protein ligase RING1-like [Cucurbita moschata])

HSP 1 Score: 613 bits (1581), Expect = 9.16e-221
Identity = 323/324 (99.69%), Postives = 323/324 (99.69%), Query Frame = 0

Query: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60
           MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE
Sbjct: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60

Query: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120
           HMDFTIPTSSSSISDHP SSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA
Sbjct: 61  HMDFTIPTSSSSISDHPGSSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120

Query: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180
           DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL
Sbjct: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180

Query: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240
           PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG
Sbjct: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240

Query: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300
           LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV
Sbjct: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300

Query: 301 NSSGDENGFVVMDGPINEDAGTLA 324
           NSSGDENGFVVMDGPINEDAGTLA
Sbjct: 301 NSSGDENGFVVMDGPINEDAGTLA 324

BLAST of Cp4.1LG12g07140 vs. NCBI nr
Match: XP_022991911.1 (E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima])

HSP 1 Score: 607 bits (1564), Expect = 3.44e-218
Identity = 320/324 (98.77%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60
           MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSS SSSLLCPHCLTDFLE
Sbjct: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSS-SSSLLCPHCLTDFLE 60

Query: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120
           HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA
Sbjct: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120

Query: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180
           DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLL+VEAKQL
Sbjct: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLDVEAKQL 180

Query: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240
           PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG
Sbjct: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240

Query: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300
           LRTTLEHMARRHSSIFSEGVQVD SQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV
Sbjct: 241 LRTTLEHMARRHSSIFSEGVQVDPSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300

Query: 301 NSSGDENGFVVMDGPINEDAGTLA 324
           NSSGDENGFV+MDGPINEDAGTLA
Sbjct: 301 NSSGDENGFVLMDGPINEDAGTLA 323

BLAST of Cp4.1LG12g07140 vs. NCBI nr
Match: KAG7014205.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 584 bits (1505), Expect = 4.71e-209
Identity = 309/310 (99.68%), Postives = 309/310 (99.68%), Query Frame = 0

Query: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60
           MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSS SSSLLCPHCLTDFLE
Sbjct: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSS-SSSLLCPHCLTDFLE 60

Query: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120
           HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA
Sbjct: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120

Query: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180
           DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL
Sbjct: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180

Query: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240
           PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG
Sbjct: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240

Query: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300
           LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV
Sbjct: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300

Query: 301 NSSGDENGFV 310
           NSSGDENGFV
Sbjct: 301 NSSGDENGFV 309

BLAST of Cp4.1LG12g07140 vs. NCBI nr
Match: KAG6575655.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 560 bits (1443), Expect = 1.25e-197
Identity = 297/299 (99.33%), Postives = 297/299 (99.33%), Query Frame = 0

Query: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60
           MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSS SSSLLCPHCLTDFLE
Sbjct: 148 MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSS-SSSLLCPHCLTDFLE 207

Query: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120
           HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA
Sbjct: 208 HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 267

Query: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180
           DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL
Sbjct: 268 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 327

Query: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240
           PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG
Sbjct: 328 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 387

Query: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVI 299
           LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGI I
Sbjct: 388 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIDI 445

BLAST of Cp4.1LG12g07140 vs. ExPASy TrEMBL
Match: A0A6J1GMD4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111455823 PE=4 SV=1)

HSP 1 Score: 613 bits (1581), Expect = 4.44e-221
Identity = 323/324 (99.69%), Postives = 323/324 (99.69%), Query Frame = 0

Query: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60
           MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE
Sbjct: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60

Query: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120
           HMDFTIPTSSSSISDHP SSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA
Sbjct: 61  HMDFTIPTSSSSISDHPGSSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120

Query: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180
           DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL
Sbjct: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180

Query: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240
           PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG
Sbjct: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240

Query: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300
           LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV
Sbjct: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300

Query: 301 NSSGDENGFVVMDGPINEDAGTLA 324
           NSSGDENGFVVMDGPINEDAGTLA
Sbjct: 301 NSSGDENGFVVMDGPINEDAGTLA 324

BLAST of Cp4.1LG12g07140 vs. ExPASy TrEMBL
Match: A0A6J1JS41 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488408 PE=4 SV=1)

HSP 1 Score: 607 bits (1564), Expect = 1.67e-218
Identity = 320/324 (98.77%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60
           MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSS SSSLLCPHCLTDFLE
Sbjct: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSS-SSSLLCPHCLTDFLE 60

Query: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120
           HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA
Sbjct: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120

Query: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180
           DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLL+VEAKQL
Sbjct: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLDVEAKQL 180

Query: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240
           PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG
Sbjct: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240

Query: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300
           LRTTLEHMARRHSSIFSEGVQVD SQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV
Sbjct: 241 LRTTLEHMARRHSSIFSEGVQVDPSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300

Query: 301 NSSGDENGFVVMDGPINEDAGTLA 324
           NSSGDENGFV+MDGPINEDAGTLA
Sbjct: 301 NSSGDENGFVLMDGPINEDAGTLA 323

BLAST of Cp4.1LG12g07140 vs. ExPASy TrEMBL
Match: A0A5D3CET0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001260 PE=4 SV=1)

HSP 1 Score: 531 bits (1369), Expect = 1.19e-188
Identity = 282/323 (87.31%), Postives = 298/323 (92.26%), Query Frame = 0

Query: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60
           MSSA+    AAAERHTYWCHECDMSVTLVSPSSSSSSSSS   SSSSSLLCPHCLTDFLE
Sbjct: 1   MSSATITATAAAERHTYWCHECDMSVTLVSPSSSSSSSSS---SSSSSLLCPHCLTDFLE 60

Query: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120
           HMDFTIPTSSSSISDHP SSSSP DSDPSSFV+VDPLP TSDDNYLL+SPQFLRLFQ LA
Sbjct: 61  HMDFTIPTSSSSISDHPNSSSSPTDSDPSSFVVVDPLPITSDDNYLLNSPQFLRLFQHLA 120

Query: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180
           DSS+SDF PSVPFNPFTPIKASVMAIPTIKV+SA+L+EDP+LICAICKD+FLLEVEAKQL
Sbjct: 121 DSSESDFVPSVPFNPFTPIKASVMAIPTIKVTSALLDEDPVLICAICKDQFLLEVEAKQL 180

Query: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240
           PCSHLYHPDCILPWLSNHDSCPLCR+KLPSD+PSD VRCR  T ALLRARDLM QEDSYG
Sbjct: 181 PCSHLYHPDCILPWLSNHDSCPLCRFKLPSDDPSDRVRCR--TSALLRARDLMHQEDSYG 240

Query: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300
           LRTTLE MARRHSSI SEG+ VDS QSPTQ GVA+M +GEQTDSVETVSSVATDDGIVIV
Sbjct: 241 LRTTLELMARRHSSISSEGIHVDSFQSPTQFGVAMMRSGEQTDSVETVSSVATDDGIVIV 300

Query: 301 NSSGDENGFVVMDGPINEDAGTL 323
           NS+GDENGFV MDGPI EDAGT+
Sbjct: 301 NSNGDENGFVGMDGPITEDAGTV 318

BLAST of Cp4.1LG12g07140 vs. ExPASy TrEMBL
Match: A0A1S3CE92 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499880 PE=4 SV=1)

HSP 1 Score: 530 bits (1365), Expect = 4.83e-188
Identity = 282/323 (87.31%), Postives = 298/323 (92.26%), Query Frame = 0

Query: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60
           MSSA+   AAAAERHTYWCHECDMSVTLVSPSSSSSSSSS   SSSSSLLCPHCLTDFLE
Sbjct: 1   MSSATITAAAAAERHTYWCHECDMSVTLVSPSSSSSSSSS---SSSSSLLCPHCLTDFLE 60

Query: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120
           HMDFTIPTSSSSISDHP SSSSP DSDPSSFV+VDPLP TSDDNYLL+SPQFLRLFQ LA
Sbjct: 61  HMDFTIPTSSSSISDHPNSSSSPTDSDPSSFVVVDPLPITSDDNYLLNSPQFLRLFQHLA 120

Query: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180
           DSS+SDF PSVPFNPFTPIKASVMAIPTIKV+SA+L+ED +LICAICKD+FLLEVEAKQL
Sbjct: 121 DSSESDFVPSVPFNPFTPIKASVMAIPTIKVTSALLDEDQVLICAICKDQFLLEVEAKQL 180

Query: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240
           PCSHLYHPDCILPWLSNHDSCPLCR+KLPSD+PSD VRCR  T ALLRARDLM QEDSYG
Sbjct: 181 PCSHLYHPDCILPWLSNHDSCPLCRFKLPSDDPSDRVRCR--TSALLRARDLMHQEDSYG 240

Query: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300
           LRTTLE MARRHSSI SEG+ VDS QSPTQ GVA+M +GEQTDSVETVSSVATDDGIVIV
Sbjct: 241 LRTTLELMARRHSSISSEGINVDSFQSPTQFGVAMMRSGEQTDSVETVSSVATDDGIVIV 300

Query: 301 NSSGDENGFVVMDGPINEDAGTL 323
           NS+GDENGFV MDGPI EDAGT+
Sbjct: 301 NSNGDENGFVGMDGPITEDAGTV 318

BLAST of Cp4.1LG12g07140 vs. ExPASy TrEMBL
Match: A0A0A0KBK3 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G429620 PE=4 SV=1)

HSP 1 Score: 524 bits (1349), Expect = 1.37e-185
Identity = 278/323 (86.07%), Postives = 297/323 (91.95%), Query Frame = 0

Query: 1   MSSASAATAAAAERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTDFLE 60
           MSSA+    AAAERHTYWCHECDMSVTLVSPSSSSSSSSSS  SSSSSL+CPHCLTDF+E
Sbjct: 1   MSSATITATAAAERHTYWCHECDMSVTLVSPSSSSSSSSSS--SSSSSLVCPHCLTDFIE 60

Query: 61  HMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQLA 120
           HMDFTIPTSSSSISD+P SSS P DSDPSSFV VDPLP TSDDNYLL+SPQFLRLFQ LA
Sbjct: 61  HMDFTIPTSSSSISDNPNSSSPPTDSDPSSFVFVDPLPITSDDNYLLNSPQFLRLFQHLA 120

Query: 121 DSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKDKFLLEVEAKQL 180
           DSS+SDF PSVPFNPFTPIKASVMAIPTIKV+SA+L+EDP+LICAICKD+FLLEVEAKQL
Sbjct: 121 DSSESDFVPSVPFNPFTPIKASVMAIPTIKVTSALLDEDPVLICAICKDQFLLEVEAKQL 180

Query: 181 PCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSDHVRCRGATMALLRARDLMPQEDSYG 240
           PCSHLYHPDCILPWLSNHDSCPLCR+KLPSD+PSD VRCR  T ALLRARDLM QEDSYG
Sbjct: 181 PCSHLYHPDCILPWLSNHDSCPLCRFKLPSDDPSDRVRCR--TSALLRARDLMHQEDSYG 240

Query: 241 LRTTLEHMARRHSSIFSEGVQVDSSQSPTQLGVAVMGNGEQTDSVETVSSVATDDGIVIV 300
           LRTTLE MARRHSSI SEG+ VDS QSPTQ GVA+M +GEQTDSVETVSSVATDDGIVIV
Sbjct: 241 LRTTLELMARRHSSISSEGIHVDSFQSPTQFGVAMMESGEQTDSVETVSSVATDDGIVIV 300

Query: 301 NSSGDENGFVVMDGPINEDAGTL 323
           NS+GDENGF+ MDGPI EDAGT+
Sbjct: 301 NSNGDENGFLGMDGPITEDAGTV 319

BLAST of Cp4.1LG12g07140 vs. TAIR 10
Match: AT3G60080.1 (RING/U-box superfamily protein )

HSP 1 Score: 172.2 bits (435), Expect = 7.0e-43
Identity = 110/277 (39.71%), Postives = 158/277 (57.04%), Query Frame = 0

Query: 1   MSSASAATAAA---AERHTYWCHECDMSVTLVSPSSSSSSSSSSSSSSSSSLLCPHCLTD 60
           MSS++  T       ER TYWCHECDMS++L+        SSS S S SS LLCP C  D
Sbjct: 1   MSSSTTTTTTVHGEPERRTYWCHECDMSLSLL--------SSSDSDSDSSPLLCPQCRVD 60

Query: 61  FLEHMDFTIPTSSSSISDHPISSSSPPD------SDPSSFVIVDPLPTTSDDNYLLSSPQ 120
           FLE MD    +SSS++ D  I      D       D   +  VDP    SDDN+LL SP 
Sbjct: 61  FLERMDH--DSSSSNLFDVTIGDFEEQDGENDDEDDEEDWCFVDP-AVNSDDNFLLDSPY 120

Query: 121 FLRLFQQLADSSDSDFAPSVPFNPFTPIKAS-VMAIPTIKVSSAML------EEDPILIC 180
             RL + LA  +    + S   +  + +K+S + +IPTI++SS++L      + D +L+C
Sbjct: 121 LHRLLRHLASDNSGSSSSSSSSSSSSLLKSSDIDSIPTIQISSSLLCSTDDSDPDSVLLC 180

Query: 181 AICKDKFLLEVEAKQLPCSHLYHPDCILPWLSNHDSCPLCRYKLPSDE------PSDHVR 240
           A+CK+ F++   A++LPCSH+YH DCI+PWLS+H+SCPLCR++LP+            +R
Sbjct: 181 AVCKEDFIIGESARRLPCSHIYHSDCIVPWLSDHNSCPLCRFELPTTAKVGIGGSEAEMR 240

Query: 241 CRGATMALLRARDLMPQEDSYGLRTTLEHMARRHSSI 256
            R + +A + A     ++D  G+R  L  +ARRH  +
Sbjct: 241 IRLSDLATIAADGDDVEDDWLGIRNALRRLARRHEQM 266

BLAST of Cp4.1LG12g07140 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 90.9 bits (224), Expect = 2.0e-18
Identity = 44/101 (43.56%), Postives = 62/101 (61.39%), Query Frame = 0

Query: 110 PQFLRLFQQLADSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKD 169
           P    L +QL+  +     P  P       K+S+ A+PTIK++   L+      C +CKD
Sbjct: 140 PGLEELIEQLSSGTHHRGPPPAP-------KSSIDALPTIKITQKHLKSSD-SHCPVCKD 199

Query: 170 KFLLEVEAKQLPCSHLYHPDCILPWLSNHDSCPLCRYKLPS 211
           +F L+ EAKQ+PC H+YH DCI+PWL  H+SCP+CR +LPS
Sbjct: 200 EFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPS 232

BLAST of Cp4.1LG12g07140 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 90.9 bits (224), Expect = 2.0e-18
Identity = 44/101 (43.56%), Postives = 62/101 (61.39%), Query Frame = 0

Query: 110 PQFLRLFQQLADSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKD 169
           P    L +QL+  +     P  P       K+S+ A+PTIK++   L+      C +CKD
Sbjct: 140 PGLEELIEQLSSGTHHRGPPPAP-------KSSIDALPTIKITQKHLKSSD-SHCPVCKD 199

Query: 170 KFLLEVEAKQLPCSHLYHPDCILPWLSNHDSCPLCRYKLPS 211
           +F L+ EAKQ+PC H+YH DCI+PWL  H+SCP+CR +LPS
Sbjct: 200 EFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPS 232

BLAST of Cp4.1LG12g07140 vs. TAIR 10
Match: AT3G56580.3 (RING/U-box superfamily protein )

HSP 1 Score: 90.9 bits (224), Expect = 2.0e-18
Identity = 44/101 (43.56%), Postives = 62/101 (61.39%), Query Frame = 0

Query: 110 PQFLRLFQQLADSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAMLEEDPILICAICKD 169
           P    L +QL+  +     P  P       K+S+ A+PTIK++   L+      C +CKD
Sbjct: 140 PGLEELIEQLSSGTHHRGPPPAP-------KSSIDALPTIKITQKHLKSSD-SHCPVCKD 199

Query: 170 KFLLEVEAKQLPCSHLYHPDCILPWLSNHDSCPLCRYKLPS 211
           +F L+ EAKQ+PC H+YH DCI+PWL  H+SCP+CR +LPS
Sbjct: 200 EFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPS 232

BLAST of Cp4.1LG12g07140 vs. TAIR 10
Match: AT2G44330.1 (RING/U-box superfamily protein )

HSP 1 Score: 90.1 bits (222), Expect = 3.5e-18
Identity = 57/161 (35.40%), Postives = 84/161 (52.17%), Query Frame = 0

Query: 59  LEHMDFTIPTSSSSISDHPISSSSPPDSDPSSFVIVDPLPTTSDDNYLLSSPQFLRLFQQ 118
           LE  D+T P  +  +    + S+SPP             P  +  +Y  SS  F      
Sbjct: 10  LEMRDYTCPECNIGLRVLSLPSASPP-----------YCPLCNVASYFTSSTPFEVGPNP 69

Query: 119 LADSSDSDFAPSVPFNPFTPIKASVMAIPTIKVSSAML----EEDPILICAICKDKFLLE 178
             D  +S F          P++    ++PTIK+SS+ML     +D  L CAIC++ F++ 
Sbjct: 70  FEDDEESQF--------LDPME----SLPTIKISSSMLSSASSDDSALPCAICREDFVVG 129

Query: 179 VEAKQLPCSHLYHPDCILPWLSNHDSCPLCRYKLPSDEPSD 216
             A++LPC+HLYH DCI+PWL++H+SCPLCR +LP     D
Sbjct: 130 ESARRLPCNHLYHNDCIIPWLTSHNSCPLCRVELPVASSED 147

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94AK42.9e-1743.56E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
O221976.4e-1742.57Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
P0CH301.0e-1432.37E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Q8LPN71.9e-1330.15E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
O222833.3e-1330.04Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Match NameE-valueIdentityDescription
XP_023549060.15.53e-222100.00E3 ubiquitin-protein ligase RING1-like [Cucurbita pepo subsp. pepo][more]
XP_022953211.19.16e-22199.69E3 ubiquitin-protein ligase RING1-like [Cucurbita moschata][more]
XP_022991911.13.44e-21898.77E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima][more]
KAG7014205.14.71e-20999.68E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6575655.11.25e-19799.33E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A6J1GMD44.44e-22199.69RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111455823... [more]
A0A6J1JS411.67e-21898.77RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488408 P... [more]
A0A5D3CET01.19e-18887.31RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CE924.83e-18887.31RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499880 PE=4 ... [more]
A0A0A0KBK31.37e-18586.07RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G429620 PE... [more]
Match NameE-valueIdentityDescription
AT3G60080.17.0e-4339.71RING/U-box superfamily protein [more]
AT3G56580.22.0e-1843.56RING/U-box superfamily protein [more]
AT3G56580.12.0e-1843.56RING/U-box superfamily protein [more]
AT3G56580.32.0e-1843.56RING/U-box superfamily protein [more]
AT2G44330.13.5e-1835.40RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 164..204
e-value: 2.4E-6
score: 37.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 164..205
e-value: 4.5E-10
score: 39.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 164..205
score: 12.164712
IPR039525E3 ubiquitin-protein ligase RNF126-like, zinc-ribbonPFAMPF14369zinc_ribbon_9coord: 16..62
e-value: 7.1E-8
score: 32.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 136..211
e-value: 1.2E-17
score: 65.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..93
NoneNo IPR availablePANTHERPTHR15710:SF89FINGER PROTEIN, PUTATIVE-RELATEDcoord: 1..280
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 1..280
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 146..211

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG12g07140.1Cp4.1LG12g07140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
molecular_function GO:0061630 ubiquitin protein ligase activity