CSPI05G07210.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI05G07210.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Cytochrome p450
Location	Chr5: 6316866 .. 6320125 (+)
Sequence length	1434
RNA-Seq Expression	CSPI05G07210.1
Synteny	CSPI05G07210.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAGGATGAATGCAAACAACCCTAATAGTACAGTTGAAGATTCCTCCTCTCATCATCAACTTACTCACGATATTCATTCCACTGTCTTCCCGTATTTTGCAAGGTGGCAAACTACGTACGGTAAGCATTCTTATATATAAGAGAAACGACATTGTTATATGATTCATTCTTCTTCATTGATTTAATTTTAATTTCCCTCTTTAAATCTTTAGATATTACTAATGTTTTTAGCATTTTCTTTATTGAATAGTTGGACTTCATGCATACGCAGAAGTTGATGTCAGCTGAGTTTATACGTTCACGTCGGTGAATTGTTTTAAGTATTTTCTTATAGAGAAATATACGATTAGATCAGGGATGAATAATCATAATTAGTAGGGGTGCAATGGAATACTTCAACTGGAATTTTAACGTTGATTGACGATGAATTGTGTATGTTATTGCAGGAAAAGTATTTACATATTGGTTGGGGACAGAGCCATTCTTGTATATAGCAGAACCAGAATTAGTGAAGATATTATCAAAAGAAGTAAAAGCAAAGCATTGGGGAAAGCCATCAGTGTTCAAAAATGACAGAAAATCAATGTTTGGAAATGGTTTGGTAATGGCTGAAGGAGATGAATGGGTTCGCCAAAGACATATCATTACTCCTGCGTTTAATCCTTCCAACTTGAAGGTACGTTTTTCTTGAAAATCACTTTTGAGTCTTTAAATTTTCAAAGAACTTATAACCCTTTAGTTCCTACTTTTTCTTTAAATTGTGATATCTTAGTCCTAGTAACAATTTAACCATATCTATCTTTAAATTTAAAAAACAGTTTAGATTTTATCTTCAAATTTAAGCATTTGTAACTAAACTTATACCTTGATTAATTTATTAAATCACGTAATCTTAACATTAAAAGTATAGAGACTAAATCGTTGTTAACTACACTTTAGAGATTAAATTGTTACGTTATAGAAAGTTCGACTAGCAAAATTTTGAAGACTATTATCATTTAAACAACACCTTTCTGTACTTTTTTGTTGTGGAGATGCTAAAAGGGCATTTTCAAAATTGTACACATCCACTTTTTGAAAATAAAATGTATTTGTCGGTTTTTTAATTTTTAAAGAGTTGTTTTTATATTTAACAAAATGACTAACTTACGGACAAAAAGTTATAATTAAAAACCTTTAAAACATGTCACATCCAAACGCACTTATAAGATACATACGTATGCACGGATAAATGTTGTCGAATGTGTTCTATATTTTCTCAAGTTTTTTAACATTTCAAACACATTATTGAATAGTCGAAACATGCAAAATAAAAAATAATTAAATACATATCAATGAGTTTTAAGAAATTATAAATATAACAAAATCTATCGATATCGAACTTTATCGTGTTGGACTCTTATTCACTTATTCCTAAGAAAATTGTAATACTTTACATTTTACTAATATTTTGAGTTAAAACAGGCCATGGCAAGCTTAATGGTGGAGTCCGCCACCCAAATGGTGGAGAGATGGGCTAACCTTATCATCTCCGGCAATCCTCAAATCGAAGTCGAGCAGGAGATAACTTCCACCGCCGGCGAAATCATTGCCAAAACCAGCTTTGGAATCCATCATGAAAGCGGCCGACAAGTCTTCAACAAACTCCGCCAACTCCAATTCACTCTATTCAAAACCAACCGCCTTGTCGGCGTCCCCTTCGCGGGGCTTTTAAACGCCGCCAAGGCCCGGGAGGCGAAAGTCCTCGGGGAGGAGATCGACGAATTGTTTCGGTCGGTGATTGCGGAGAGGAGAGAAAATGCGGTGGCGGAGACGACGAGGACGACGGAGCAGCAGAATGATCTTTTGAGTTTGCTTTTGAAAGAGAGTAGTAGTGGCGGTGGAGGAGGAGGAGAAGGGAGGAGGCTGAGCACGGCGGAGCTTATTGATGAATGTAAAACGTTCTTTTTCGGTGGACATGAAACGACGGCGTTGGCTTTGACGTGGACTTTGATGCTATTGGCTGTTCATTCTGAATGGCAAACAATATTGAGAGAAGAAATTAAGGAAGTTTTTGGTCATAATTTACAAACTCAATTTGATTTCACTAACCTTTCTTCACTCAAAAAGGTAACTAATTAAAAACTTCTCTAATTAATTCTCCTTTTAATCTTTCATTATTCTTTAATTCTAATTTAAAACATTTACTGATTTTTTAACATGTAATGCATTTTATGAAGTAAAAGATTTGAAATTAGAGGTTAGCTAAAATCTAAAATCTAAAAACAAAATAACAAGTTTTAAAAATTACTTAGATGAATTTTAAAATTAAGTTTATAAGTTTAATGTTGAGTGTTATTCACTCTGTCAATTTTTTCAAAAGTTGAGAGAAATTTCAAAGTTTCTAAAATCACTTTTTTATTGTTATTTTTTTAGAATGTAGTTAGGAGTCTAAGTATTTCTTTTAAAATGTGATAGTCATATGAGGCTTTAAAATTTCATTAAACAAAAAATGATCTTTTCTTTCTCCTTTGAGGCTTTGATTTTTCAAAATTAGTTTTTAAATGAGAATAAGATTTACTTAACTTAATTAATGTTAACAAAGACTAAATTGTTAAAAGTTATCTCTCTAACTTATTTTCTTTCATAAGTAATTTAAAAAAAGAAATATGTTTGCAATAAAGACTTAAAAATATTCCAAGTCTTTTAAAAATTATAATATTGTCAAAACTATTAGGACAAAAATTGGTTAGTGTCCCACAAAAGAAATACTATTATTAGAATAGGATTTCTTGTTGTTTTTGGTTGGGTAGTGGTAATAAATAATGAACCCTTTTTGCAGATGGGATGGGTGATGAGTGAGGTCCTACGTCTATATCCATCAGCTCCAAATGTACAAAGACAAGCAAGAAAAGACATTACAATTGATGGACTCACAATCCCAAATGGGACCAACATGTGGATTGATGTTGTATCCATGCACCATGACCAAGCTCTTTGGGGTCACCAAGTCAATGAATTTCACCCCGAGAGGTTCCGAAACGACACTGTTTCAGGAGGTTGCACACACAAGATGGGTTATTTGCCCTTCGGCTTTGGAGGAAGAATGTGTGTTGGTAGGCACTTGACCTTTATGGAGTATAAGATTGTTTTGACTCTTATTTTGTCTAGGTTTTCCTTCTCTTTGTCCTCAGATTATCAACATTCGCCTTCTATAATGCTCTCGCTACGACCTGCCCATGGTCTGCCTCTTGTTTTTCGACTACTCGAAGAATAA

mRNA sequence

ATGAAAAGGATGAATGCAAACAACCCTAATAGTACAGTTGAAGATTCCTCCTCTCATCATCAACTTACTCACGATATTCATTCCACTGTCTTCCCGTATTTTGCAAGGTGGCAAACTACGTACGGAAAAGTATTTACATATTGGTTGGGGACAGAGCCATTCTTGTATATAGCAGAACCAGAATTAGTGAAGATATTATCAAAAGAAGTAAAAGCAAAGCATTGGGGAAAGCCATCAGTGTTCAAAAATGACAGAAAATCAATGTTTGGAAATGGTTTGGTAATGGCTGAAGGAGATGAATGGGTTCGCCAAAGACATATCATTACTCCTGCGTTTAATCCTTCCAACTTGAAGGCCATGGCAAGCTTAATGGTGGAGTCCGCCACCCAAATGGTGGAGAGATGGGCTAACCTTATCATCTCCGGCAATCCTCAAATCGAAGTCGAGCAGGAGATAACTTCCACCGCCGGCGAAATCATTGCCAAAACCAGCTTTGGAATCCATCATGAAAGCGGCCGACAAGTCTTCAACAAACTCCGCCAACTCCAATTCACTCTATTCAAAACCAACCGCCTTGTCGGCGTCCCCTTCGCGGGGCTTTTAAACGCCGCCAAGGCCCGGGAGGCGAAAGTCCTCGGGGAGGAGATCGACGAATTGTTTCGGTCGGTGATTGCGGAGAGGAGAGAAAATGCGGTGGCGGAGACGACGAGGACGACGGAGCAGCAGAATGATCTTTTGAGTTTGCTTTTGAAAGAGAGTAGTAGTGGCGGTGGAGGAGGAGGAGAAGGGAGGAGGCTGAGCACGGCGGAGCTTATTGATGAATGTAAAACGTTCTTTTTCGGTGGACATGAAACGACGGCGTTGGCTTTGACGTGGACTTTGATGCTATTGGCTGTTCATTCTGAATGGCAAACAATATTGAGAGAAGAAATTAAGGAAGTTTTTGGTCATAATTTACAAACTCAATTTGATTTCACTAACCTTTCTTCACTCAAAAAGATGGGATGGGTGATGAGTGAGGTCCTACGTCTATATCCATCAGCTCCAAATGTACAAAGACAAGCAAGAAAAGACATTACAATTGATGGACTCACAATCCCAAATGGGACCAACATGTGGATTGATGTTGTATCCATGCACCATGACCAAGCTCTTTGGGGTCACCAAGTCAATGAATTTCACCCCGAGAGGTTCCGAAACGACACTGTTTCAGGAGGTTGCACACACAAGATGGGTTATTTGCCCTTCGGCTTTGGAGGAAGAATGTGTGTTGGTAGGCACTTGACCTTTATGGAGTATAAGATTGTTTTGACTCTTATTTTGTCTAGGTTTTCCTTCTCTTTGTCCTCAGATTATCAACATTCGCCTTCTATAATGCTCTCGCTACGACCTGCCCATGGTCTGCCTCTTGTTTTTCGACTACTCGAAGAATAA

Coding sequence (CDS)

ATGAAAAGGATGAATGCAAACAACCCTAATAGTACAGTTGAAGATTCCTCCTCTCATCATCAACTTACTCACGATATTCATTCCACTGTCTTCCCGTATTTTGCAAGGTGGCAAACTACGTACGGAAAAGTATTTACATATTGGTTGGGGACAGAGCCATTCTTGTATATAGCAGAACCAGAATTAGTGAAGATATTATCAAAAGAAGTAAAAGCAAAGCATTGGGGAAAGCCATCAGTGTTCAAAAATGACAGAAAATCAATGTTTGGAAATGGTTTGGTAATGGCTGAAGGAGATGAATGGGTTCGCCAAAGACATATCATTACTCCTGCGTTTAATCCTTCCAACTTGAAGGCCATGGCAAGCTTAATGGTGGAGTCCGCCACCCAAATGGTGGAGAGATGGGCTAACCTTATCATCTCCGGCAATCCTCAAATCGAAGTCGAGCAGGAGATAACTTCCACCGCCGGCGAAATCATTGCCAAAACCAGCTTTGGAATCCATCATGAAAGCGGCCGACAAGTCTTCAACAAACTCCGCCAACTCCAATTCACTCTATTCAAAACCAACCGCCTTGTCGGCGTCCCCTTCGCGGGGCTTTTAAACGCCGCCAAGGCCCGGGAGGCGAAAGTCCTCGGGGAGGAGATCGACGAATTGTTTCGGTCGGTGATTGCGGAGAGGAGAGAAAATGCGGTGGCGGAGACGACGAGGACGACGGAGCAGCAGAATGATCTTTTGAGTTTGCTTTTGAAAGAGAGTAGTAGTGGCGGTGGAGGAGGAGGAGAAGGGAGGAGGCTGAGCACGGCGGAGCTTATTGATGAATGTAAAACGTTCTTTTTCGGTGGACATGAAACGACGGCGTTGGCTTTGACGTGGACTTTGATGCTATTGGCTGTTCATTCTGAATGGCAAACAATATTGAGAGAAGAAATTAAGGAAGTTTTTGGTCATAATTTACAAACTCAATTTGATTTCACTAACCTTTCTTCACTCAAAAAGATGGGATGGGTGATGAGTGAGGTCCTACGTCTATATCCATCAGCTCCAAATGTACAAAGACAAGCAAGAAAAGACATTACAATTGATGGACTCACAATCCCAAATGGGACCAACATGTGGATTGATGTTGTATCCATGCACCATGACCAAGCTCTTTGGGGTCACCAAGTCAATGAATTTCACCCCGAGAGGTTCCGAAACGACACTGTTTCAGGAGGTTGCACACACAAGATGGGTTATTTGCCCTTCGGCTTTGGAGGAAGAATGTGTGTTGGTAGGCACTTGACCTTTATGGAGTATAAGATTGTTTTGACTCTTATTTTGTCTAGGTTTTCCTTCTCTTTGTCCTCAGATTATCAACATTCGCCTTCTATAATGCTCTCGCTACGACCTGCCCATGGTCTGCCTCTTGTTTTTCGACTACTCGAAGAATAA

Protein sequence

MKRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE*

Homology

[Close]

BLAST of CSPI05G07210.1 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.2e-85
Identity = 177/468 (37.82%), Postives = 265/468 (56.62%), Query Frame = 0


Query: 11  STVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEV 70
           S  +D  S H   HDI   + P++  W   YGK F  W GT+P L + E EL+    KE+
Sbjct: 66  SASKDCDSIH---HDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELI----KEL 125

Query: 71  KAKH---WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVES 130
             KH    G+  + +   K+  G GL+MA G +W  QRH+  PAF    LK  A  MVE 
Sbjct: 126 LMKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVEC 185

Query: 131 ATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQFTLF 190
            +++VER    +  G  ++E+ +E+     +II++T FG   E G+++FN L  LQ    
Sbjct: 186 TSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCA 245

Query: 191 KTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQNDLLS 250
           +  R +  P +  L +   RE K L +E++ L   +I  RR+   AE  R++   +DLL 
Sbjct: 246 QATRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRD--CAEMGRSSTHGDDLLG 305

Query: 251 LLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTIL 310
           LLL E             L    ++DECKTFFF GHETTAL LTWT MLLA +  WQ  +
Sbjct: 306 LLLNEMDIDKNNNNNNNNLQL--IMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKV 365

Query: 311 REEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDGLTIP 370
           REE++EVFG N     D   LS L  +  V++E LRLYP A  + R A +D+ +  LTIP
Sbjct: 366 REEVREVFGRNGLPSVD--QLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIP 425

Query: 371 NGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGRH 430
            G ++WI V+++HH + LWG   N+F+PERF     + G      ++PF  G R C+G+ 
Sbjct: 426 KGLSIWIPVLAIHHSEELWGKDANQFNPERFGGRPFASG----RHFIPFAAGPRNCIGQQ 485

Query: 431 LTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLL 476
              ME KI+L  ++S+F+F++S +Y+H+P ++L+++P +G+ ++ + L
Sbjct: 486 FALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

[Close]

BLAST of CSPI05G07210.1 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.2e-80
Identity = 172/470 (36.60%), Postives = 263/470 (55.96%), Query Frame = 0


Query: 10  NSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKE 69
           +S   D SS H   H+I   + P++  W   YGK F  W GTEP L + E E++    KE
Sbjct: 66  HSASNDCSSIH---HNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMI----KE 125

Query: 70  VKAKH---WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVE 129
           +  KH    GK  + +   K   G GL+MA G+ W  QRH+  PAF    LK  A  MVE
Sbjct: 126 LLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVE 185

Query: 130 SATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQFTL 189
               M ER    +     ++E+ +E+     +II++T FG   + G+++F+ L  LQ   
Sbjct: 186 CTKMMAERLRKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLC 245

Query: 190 FKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQNDLL 249
            +  R +  P +  L +   RE K L  E++ L   +I  R+++   E  R++   +DLL
Sbjct: 246 AQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDS--VEIGRSSSYGDDLL 305

Query: 250 SLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTI 309
            LLL +  S          L+   ++DECKTFFF GHETT+L LTWTLMLLA +  WQ  
Sbjct: 306 GLLLNQMDS------NKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDN 365

Query: 310 LREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDGLTI 369
           +R+E+++V G +     +   LSSL  +  V++E LRLYP A  + R A +DI +  L I
Sbjct: 366 VRDEVRQVCGQDGVPSVE--QLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLII 425

Query: 370 PNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGR 429
           P G ++WI V+++HH   LWG   NEF+PERF   + +        ++PF  G R C+G+
Sbjct: 426 PKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSFAS----SRHFMPFAAGPRNCIGQ 485

Query: 430 HLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLE 477
               ME KI+L +++S+FSF++S +Y+H+P ++L+++P +G+ LV + L+
Sbjct: 486 TFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

[Close]

BLAST of CSPI05G07210.1 vs. ExPASy Swiss-Prot
Match: Q93Z79 (Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 6.6e-79
Identity = 171/482 (35.48%), Postives = 272/482 (56.43%), Query Frame = 0


Query: 11  STVEDSSSHHQ----LTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKIL 70
           S +  +S H+     + HD  S++FPY   W+  YG+V+TY  G +  LY+  PELVK L
Sbjct: 67  SQIMSNSKHYSGDNIIAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKEL 126

Query: 71  SKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVE 130
           + +    + GK S      KS+ G G++ + G  W  QR II P F    +K M  L+VE
Sbjct: 127 N-QANTLNLGKVSYVTKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVE 186

Query: 131 SATQMVERWANLIISGNP---QIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQ 190
           SA  M+ +W  ++         I V++++ + + ++I++  FG     G+++F+KLR LQ
Sbjct: 187 SAMPMLSKWEEMMKREGEMVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQ 246

Query: 191 FTLFKTNRLVGV-PFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQ 250
             +   N L  +  F  ++   K    K    +IDEL R +     E+ + ET +  E++
Sbjct: 247 KAITHNNILFSLNGFTDVVFGTK----KHGNGKIDELERHI-----ESLIWETVKERERE 306

Query: 251 ------NDLLSLLLKESSSGGGGGGEGRRLS-TAELIDECKTFFFGGHETTALALTWTLM 310
                  DL+ L+L+ + S   G  E +  S  + ++D CK+ +F GHET+A+A++W LM
Sbjct: 307 CVGDHKKDLMQLILEGARSSCDGNLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLM 366

Query: 311 LLAVHSEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQA 370
           LLA++  WQT +R+   EVF H      D  ++S+LK +  V+ E LRLYP A  V R+A
Sbjct: 367 LLALNPSWQTRIRD---EVFLHCKNGIPDADSISNLKTVTMVIQETLRLYPPAAFVSREA 426

Query: 371 RKDITIDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLP 430
            +D  +  L +P G  +W  + ++H D  +WG   NEF+PERF ++ VS  C H   ++P
Sbjct: 427 LEDTKLGNLVVPKGVCIWTLIPTLHRDPEIWGADANEFNPERF-SEGVSKACKHPQSFVP 486

Query: 431 FGFGGRMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLL 478
           FG G R+C+G++   ME K++++LI+SRFSF+LS  YQHSP   + + P HG  +V R+L
Sbjct: 487 FGLGTRLCLGKNFGMMELKVLVSLIVSRFSFTLSPTYQHSPVFRMLVEPQHG--VVIRVL 532

[Close]

BLAST of CSPI05G07210.1 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 5.6e-78
Identity = 162/479 (33.82%), Postives = 259/479 (54.07%), Query Frame = 0


Query: 1   MKRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEP 60
           M+R+     ++  +++ S    + +  +T+FPYF  W   YG ++ Y  G+   L + +P
Sbjct: 61  MRRIQQLAKSAHEQEAGSTDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDP 120

Query: 61  ELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAM 120
            +VK L+   K+   GKP   + +R ++ G G++ + GD WV QR +I P      +K M
Sbjct: 121 NMVKELA-NCKSLDLGKPCYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGM 180

Query: 121 ASLMVESATQMVERWANLI--ISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK 180
            +LM+E+A  M+  W N +    G+ +I V++ + + + ++I++  FG     G+++F K
Sbjct: 181 VNLMMEAAMSMLNSWKNEVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIK 240

Query: 181 LRQLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRT 240
           +RQLQ  + K + L+GVP +  L     R    L   I  L  ++  +   ++      +
Sbjct: 241 IRQLQKAMAKQSMLIGVPGSRYLPTRSNRGIWNLDSSIRTLILNISKKYEHDS------S 300

Query: 241 TEQQNDLLSLLLKESSSGGGGGGEGRRLSTAE--LIDECKTFFFGGHETTALALTWTLML 300
           T    DLL  +++ S  G           T E  ++D CK  +F GHETT+    W LML
Sbjct: 301 TSVNKDLLHSIIQGSKDGPFAS------CTPEDFIVDNCKNIYFAGHETTSTTAAWCLML 360

Query: 301 LAVHSEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQAR 360
           LA H EWQ+  R E  ++         DF  L  LKK+  V+ E LRLYP A  V R+A 
Sbjct: 361 LASHHEWQSRARVESLDICQGR---PLDFDILRKLKKLTMVIQETLRLYPPASFVAREAL 420

Query: 361 KDITIDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPF 420
            D+ + G+ IP GTN+WI +   H D ++WG   ++F P+RF N  ++G C     Y+PF
Sbjct: 421 NDMKLGGIDIPKGTNIWIPIAMAHRDPSVWGPSADKFDPDRFANG-IAGACKPPHMYMPF 480

Query: 421 GFGGRMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLL 476
           G G R C G++L  +E K+VL+L+LS+F F LS +Y H P+  L++ P  G+PL+FR L
Sbjct: 481 GVGVRTCAGQNLAMVELKVVLSLLLSKFEFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

[Close]

BLAST of CSPI05G07210.1 vs. ExPASy Swiss-Prot
Match: Q5KQH7 (Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 2.6e-75
Identity = 157/463 (33.91%), Postives = 254/463 (54.86%), Query Frame = 0


Query: 25  DIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEVKAKHWGKPSVFKND 84
           D  + +FPYF +W+  YG+ + YWL   P LY+ +PEL+  + + V +   GKP   +  
Sbjct: 102 DYCTRIFPYFHKWRKAYGETYLYWLRRRPALYVTDPELIGEIGRCV-SLDMGKPKYLQKG 161

Query: 85  RKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVESATQMVERWANLIISGN- 144
           ++ +FG G++ A G  W RQR +I P F  + ++AM  LMV++A  ++  W + I +   
Sbjct: 162 QEPLFGGGVLKANGACWARQRKVIAPEFYMARVRAMVQLMVDAAQPLIASWESRIDAAGG 221

Query: 145 ---PQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQFTLFKTNRLVGVPFAGL 204
               ++ V+ ++ S + ++I++  FG  +  GR++F +LR+L   + +T+ +  +P    
Sbjct: 222 AAAAEVVVDGDLRSFSFDVISRACFGSDYSRGREIFLRLRELSGLMSETSVIFSIPSLRH 281

Query: 205 LNAAKAREAKVLGEEIDELFRSVIAERRENAVA------ETTRTTEQQNDLLSLLLKESS 264
           L   K R    L  EI  L   ++ ERR  A A      +    +  + D L  +++ S 
Sbjct: 282 LPTGKNRRIWRLTGEIRSLIMELVRERRCAARAAREHGGKAAPPSPPERDFLGSIIENS- 341

Query: 265 SGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTILREEIKEV 324
                GG+ R      ++D CK  +F GHET+A+  TW LMLLA H EWQ   R E+ EV
Sbjct: 342 -----GGQPR--PDDFVVDNCKNIYFAGHETSAVTATWCLMLLAAHPEWQDRARAEVLEV 401

Query: 325 FGHN---LQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDGLTIPNGTN 384
            G +        DF  +S ++ +G V+ E LRL+P +  V R+  +D+ +  L  P GT 
Sbjct: 402 CGGDGAAAPAAPDFDMVSRMRTVGMVVQETLRLFPPSSFVVRETFRDMQLGRLLAPKGTY 461

Query: 385 MWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTH-KMGYLPFGFGGRMCVGRHLTF 444
           +++ V +MHHD A WG     F P RFR D V+  C H +  ++PFG G R C+G++L  
Sbjct: 462 LFVPVSTMHHDVAAWGPTARLFDPSRFR-DGVAAACKHPQASFMPFGLGARTCLGQNLAL 521

Query: 445 MEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFR 474
           +E K ++ ++L+RF F+LS +Y+HSP+  L + P  GL L  R
Sbjct: 522 VEVKTLVAVVLARFEFTLSPEYRHSPAFRLIIEPEFGLRLRIR 554

[Close]

BLAST of CSPI05G07210.1 vs. ExPASy TrEMBL
Match: A0A0A0KKS5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166390 PE=3 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 9.7e-275
Identity = 473/474 (99.79%), Postives = 473/474 (99.79%), Query Frame = 0


Query: 4   MNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELV 63
           MNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELV
Sbjct: 1   MNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELV 60

Query: 64  KILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASL 123
           KILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASL
Sbjct: 61  KILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASL 120

Query: 124 MVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQ 183
           MVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFG HHESGRQVFNKLRQLQ
Sbjct: 121 MVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGFHHESGRQVFNKLRQLQ 180

Query: 184 FTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQN 243
           FTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQN
Sbjct: 181 FTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQN 240

Query: 244 DLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEW 303
           DLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEW
Sbjct: 241 DLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEW 300

Query: 304 QTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDG 363
           QTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDG
Sbjct: 301 QTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDG 360

Query: 364 LTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMC 423
           LTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMC
Sbjct: 361 LTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMC 420

Query: 424 VGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE 478
           VGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE
Sbjct: 421 VGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE 474

[Close]

BLAST of CSPI05G07210.1 vs. ExPASy TrEMBL
Match: E5GCU7 (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.6e-256
Identity = 448/479 (93.53%), Postives = 459/479 (95.82%), Query Frame = 0


Query: 1   MKRMNANNPNSTVEDSSSHHQ--LTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA 60
           MK  N NNPNST EDS SH    +TH+IHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA
Sbjct: 36  MKMKNVNNPNSTFEDSFSHDDQLITHNIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA 95

Query: 61  EPELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 120
           +PE VKILSKEVKAK+WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK
Sbjct: 96  DPEFVKILSKEVKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 155

Query: 121 AMASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK 180
           AMASLMVESAT+MVERWANL+ISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK
Sbjct: 156 AMASLMVESATKMVERWANLVISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK 215

Query: 181 LRQLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRT 240
           LRQLQ TLFKTNRLVGVPF GLLNAAKAREAK+LGEEIDELFRSVI ERRE+AVAE T T
Sbjct: 216 LRQLQITLFKTNRLVGVPFVGLLNAAKAREAKLLGEEIDELFRSVITERREDAVAERT-T 275

Query: 241 TEQQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA 300
           TEQQNDLLSLLLKE SSGGGGG EGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA
Sbjct: 276 TEQQNDLLSLLLKE-SSGGGGGEEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA 335

Query: 301 VHSEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD 360
           VHSEWQTILREEIKEVFGHNLQT FDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD
Sbjct: 336 VHSEWQTILREEIKEVFGHNLQTHFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD 395

Query: 361 ITIDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF 420
           I I+GLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF
Sbjct: 396 IIINGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF 455

Query: 421 GGRMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE 478
           GGRMCVGRHLTFMEYKIVLTLILSRFSFSLS DY+HSPSIMLSLRPAHGLPLVFRLLE+
Sbjct: 456 GGRMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYRHSPSIMLSLRPAHGLPLVFRLLED 512

[Close]

BLAST of CSPI05G07210.1 vs. ExPASy TrEMBL
Match: A0A1S4DS94 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 1.6e-256
Identity = 448/479 (93.53%), Postives = 459/479 (95.82%), Query Frame = 0


Query: 1   MKRMNANNPNSTVEDSSSHHQ--LTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA 60
           MK  N NNPNST EDS SH    +TH+IHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA
Sbjct: 63  MKMKNVNNPNSTFEDSFSHDDQLITHNIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA 122

Query: 61  EPELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 120
           +PE VKILSKEVKAK+WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK
Sbjct: 123 DPEFVKILSKEVKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 182

Query: 121 AMASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK 180
           AMASLMVESAT+MVERWANL+ISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK
Sbjct: 183 AMASLMVESATKMVERWANLVISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK 242

Query: 181 LRQLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRT 240
           LRQLQ TLFKTNRLVGVPF GLLNAAKAREAK+LGEEIDELFRSVI ERRE+AVAE T T
Sbjct: 243 LRQLQITLFKTNRLVGVPFVGLLNAAKAREAKLLGEEIDELFRSVITERREDAVAERT-T 302

Query: 241 TEQQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA 300
           TEQQNDLLSLLLKE SSGGGGG EGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA
Sbjct: 303 TEQQNDLLSLLLKE-SSGGGGGEEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA 362

Query: 301 VHSEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD 360
           VHSEWQTILREEIKEVFGHNLQT FDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD
Sbjct: 363 VHSEWQTILREEIKEVFGHNLQTHFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD 422

Query: 361 ITIDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF 420
           I I+GLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF
Sbjct: 423 IIINGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF 482

Query: 421 GGRMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE 478
           GGRMCVGRHLTFMEYKIVLTLILSRFSFSLS DY+HSPSIMLSLRPAHGLPLVFRLLE+
Sbjct: 483 GGRMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYRHSPSIMLSLRPAHGLPLVFRLLED 539

[Close]

BLAST of CSPI05G07210.1 vs. ExPASy TrEMBL
Match: A0A6J1H0W5 (cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459387 PE=3 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 1.9e-214
Identity = 374/456 (82.02%), Postives = 408/456 (89.47%), Query Frame = 0


Query: 22  LTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEVKAKHWGKPSVF 81
           LTHDIHS VFPYF+RWQ  +GKVFTYWLGTEPFLY+AEPE VK+LS+EV+AK+WGKPSVF
Sbjct: 69  LTHDIHSIVFPYFSRWQAAHGKVFTYWLGTEPFLYVAEPEFVKMLSREVQAKYWGKPSVF 128

Query: 82  KNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVESATQMVERWANLIIS 141
           K DRKSMFGNGLVMAEGDEWVRQRH+ITPAFNPSNLKAMASLMVES TQM++RWA+L++S
Sbjct: 129 KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLS 188

Query: 142 GNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQFTLFKTNRLVGVPFAGLL 201
           G+PQIEVE EITSTAGEIIAKTSFGI H SGRQVF+KLR LQ TLFKTNRLVGVPFAG+L
Sbjct: 189 GHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGIL 248

Query: 202 NAAKAREAKVLGEEIDELFRSVIAERREN-AVAETTRTTEQQNDLLSLLLKESSSGGGGG 261
           NA KAREAK LGEEIDELFR VI  RREN A AE      QQNDLLSLLLKES   GG G
Sbjct: 249 NAGKAREAKRLGEEIDELFREVITARRENQAAAEVV----QQNDLLSLLLKES---GGEG 308

Query: 262 GEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTILREEIKEVFGHNLQ 321
             GR LST ELIDECKTFFFGGHETTALALTWTL+LL +H+EWQT LR+EIKEV G + +
Sbjct: 309 KLGRWLSTTELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQTQLRDEIKEVLG-DKE 368

Query: 322 TQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDGLTIPNGTNMWIDVVSMH 381
           + FDFT LS LKKMGWVMSEVLRLYPSAPNVQRQAR+DIT++GLTIPNGTNMWIDVV+MH
Sbjct: 369 SDFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMH 428

Query: 382 HDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLI 441
           HD+ALWG QVNEF PERF +D V+GGC+HKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLI
Sbjct: 429 HDEALWGEQVNEFRPERFEHDGVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLI 488

Query: 442 LSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLE 477
           L RFSF+LS DY HSP IMLSLRPAHGLPL+F+LLE
Sbjct: 489 LCRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFQLLE 516

[Close]

BLAST of CSPI05G07210.1 vs. ExPASy TrEMBL
Match: A0A6J1K6M8 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491149 PE=3 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 4.3e-214
Identity = 370/455 (81.32%), Postives = 404/455 (88.79%), Query Frame = 0


Query: 22  LTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEVKAKHWGKPSVF 81
           LTHDIHS VFPYF+RWQ  YGK+FTYWLGTEPFLY+A+PE VK+LS+EV+AK+WGKPSVF
Sbjct: 69  LTHDIHSIVFPYFSRWQAAYGKIFTYWLGTEPFLYLADPEFVKMLSREVQAKYWGKPSVF 128

Query: 82  KNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVESATQMVERWANLIIS 141
           K DRKSMFGNGLVMAEGDEWVRQRH+ITPAFNPSNLKAMASLMVES TQM++RWA+L++S
Sbjct: 129 KRDRKSMFGNGLVMAEGDEWVRQRHVITPAFNPSNLKAMASLMVESTTQMLDRWAHLVLS 188

Query: 142 GNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQFTLFKTNRLVGVPFAGLL 201
           G+PQIEVE EITSTAGEIIAKTSFGI H SGRQVF+KLR LQ TLFKTNRLVGVPFAG+L
Sbjct: 189 GHPQIEVENEITSTAGEIIAKTSFGIDHISGRQVFHKLRNLQMTLFKTNRLVGVPFAGIL 248

Query: 202 NAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQNDLLSLLLKESSSGGGGGG 261
           NA KAREAK LGEEIDELFR VI  RREN  A        QNDLLSLLLKES   GG G 
Sbjct: 249 NAGKAREAKRLGEEIDELFREVITARRENQSATAAAEVVLQNDLLSLLLKES---GGEGK 308

Query: 262 EGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTILREEIKEVFGHNLQT 321
             R LSTAELIDECKTFFFGGHETTALALTWTL+LL +H+EWQ  LR+EIKEV G + ++
Sbjct: 309 LRRWLSTAELIDECKTFFFGGHETTALALTWTLLLLGIHTEWQIQLRDEIKEVLG-DKES 368

Query: 322 QFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDGLTIPNGTNMWIDVVSMHH 381
            FDFT LS LKKMGWVMSEVLRLYPSAPNVQRQAR+DIT++GLTIPNGTNMWIDVV+MHH
Sbjct: 369 DFDFTKLSQLKKMGWVMSEVLRLYPSAPNVQRQARRDITVNGLTIPNGTNMWIDVVAMHH 428

Query: 382 DQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLIL 441
           D+ALWG QVNEF PERF +D V+GGC+HKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLIL
Sbjct: 429 DEALWGEQVNEFRPERFEHDEVAGGCSHKMGYLPFGFGGRMCVGRHLTFMEYKIVLTLIL 488

Query: 442 SRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLE 477
            RFSF+LS DY HSP IMLSLRPAHGLPL+FRLLE
Sbjct: 489 CRFSFTLSPDYCHSPCIMLSLRPAHGLPLIFRLLE 519

[Close]

BLAST of CSPI05G07210.1 vs. NCBI nr
Match: XP_011654827.1 (cytokinin hydroxylase [Cucumis sativus])

HSP 1 Score: 961.1 bits (2483), Expect = 3.7e-276
Identity = 476/477 (99.79%), Postives = 476/477 (99.79%), Query Frame = 0


Query: 1   MKRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEP 60
           MKRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEP
Sbjct: 59  MKRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEP 118

Query: 61  ELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAM 120
           ELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAM
Sbjct: 119 ELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAM 178

Query: 121 ASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLR 180
           ASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFG HHESGRQVFNKLR
Sbjct: 179 ASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGFHHESGRQVFNKLR 238

Query: 181 QLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTE 240
           QLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTE
Sbjct: 239 QLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTE 298

Query: 241 QQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVH 300
           QQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVH
Sbjct: 299 QQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVH 358

Query: 301 SEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIT 360
           SEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIT
Sbjct: 359 SEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIT 418

Query: 361 IDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGG 420
           IDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGG
Sbjct: 419 IDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGG 478

Query: 421 RMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE 478
           RMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE
Sbjct: 479 RMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE 535

[Close]

BLAST of CSPI05G07210.1 vs. NCBI nr
Match: KAE8647911.1 (hypothetical protein Csa_000052 [Cucumis sativus])

HSP 1 Score: 961.1 bits (2483), Expect = 3.7e-276
Identity = 476/477 (99.79%), Postives = 476/477 (99.79%), Query Frame = 0


Query: 1   MKRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEP 60
           MKRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEP
Sbjct: 1   MKRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEP 60

Query: 61  ELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAM 120
           ELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAM
Sbjct: 61  ELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAM 120

Query: 121 ASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLR 180
           ASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFG HHESGRQVFNKLR
Sbjct: 121 ASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGFHHESGRQVFNKLR 180

Query: 181 QLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTE 240
           QLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTE
Sbjct: 181 QLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTE 240

Query: 241 QQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVH 300
           QQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVH
Sbjct: 241 QQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVH 300

Query: 301 SEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIT 360
           SEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIT
Sbjct: 301 SEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIT 360

Query: 361 IDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGG 420
           IDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGG
Sbjct: 361 IDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGG 420

Query: 421 RMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE 478
           RMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE
Sbjct: 421 RMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE 477

[Close]

BLAST of CSPI05G07210.1 vs. NCBI nr
Match: ADN34296.1 (cytochrome p450 [Cucumis melo subsp. melo])

HSP 1 Score: 894.8 bits (2311), Expect = 3.2e-256
Identity = 448/479 (93.53%), Postives = 459/479 (95.82%), Query Frame = 0


Query: 1   MKRMNANNPNSTVEDSSSHHQ--LTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA 60
           MK  N NNPNST EDS SH    +TH+IHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA
Sbjct: 36  MKMKNVNNPNSTFEDSFSHDDQLITHNIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA 95

Query: 61  EPELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 120
           +PE VKILSKEVKAK+WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK
Sbjct: 96  DPEFVKILSKEVKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 155

Query: 121 AMASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK 180
           AMASLMVESAT+MVERWANL+ISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK
Sbjct: 156 AMASLMVESATKMVERWANLVISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK 215

Query: 181 LRQLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRT 240
           LRQLQ TLFKTNRLVGVPF GLLNAAKAREAK+LGEEIDELFRSVI ERRE+AVAE T T
Sbjct: 216 LRQLQITLFKTNRLVGVPFVGLLNAAKAREAKLLGEEIDELFRSVITERREDAVAERT-T 275

Query: 241 TEQQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA 300
           TEQQNDLLSLLLKE SSGGGGG EGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA
Sbjct: 276 TEQQNDLLSLLLKE-SSGGGGGEEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA 335

Query: 301 VHSEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD 360
           VHSEWQTILREEIKEVFGHNLQT FDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD
Sbjct: 336 VHSEWQTILREEIKEVFGHNLQTHFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD 395

Query: 361 ITIDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF 420
           I I+GLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF
Sbjct: 396 IIINGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF 455

Query: 421 GGRMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE 478
           GGRMCVGRHLTFMEYKIVLTLILSRFSFSLS DY+HSPSIMLSLRPAHGLPLVFRLLE+
Sbjct: 456 GGRMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYRHSPSIMLSLRPAHGLPLVFRLLED 512

[Close]

BLAST of CSPI05G07210.1 vs. NCBI nr
Match: XP_016898838.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 894.8 bits (2311), Expect = 3.2e-256
Identity = 448/479 (93.53%), Postives = 459/479 (95.82%), Query Frame = 0


Query: 1   MKRMNANNPNSTVEDSSSHHQ--LTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA 60
           MK  N NNPNST EDS SH    +TH+IHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA
Sbjct: 63  MKMKNVNNPNSTFEDSFSHDDQLITHNIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIA 122

Query: 61  EPELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 120
           +PE VKILSKEVKAK+WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK
Sbjct: 123 DPEFVKILSKEVKAKNWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLK 182

Query: 121 AMASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK 180
           AMASLMVESAT+MVERWANL+ISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK
Sbjct: 183 AMASLMVESATKMVERWANLVISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNK 242

Query: 181 LRQLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRT 240
           LRQLQ TLFKTNRLVGVPF GLLNAAKAREAK+LGEEIDELFRSVI ERRE+AVAE T T
Sbjct: 243 LRQLQITLFKTNRLVGVPFVGLLNAAKAREAKLLGEEIDELFRSVITERREDAVAERT-T 302

Query: 241 TEQQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA 300
           TEQQNDLLSLLLKE SSGGGGG EGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA
Sbjct: 303 TEQQNDLLSLLLKE-SSGGGGGEEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLA 362

Query: 301 VHSEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD 360
           VHSEWQTILREEIKEVFGHNLQT FDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD
Sbjct: 363 VHSEWQTILREEIKEVFGHNLQTHFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKD 422

Query: 361 ITIDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF 420
           I I+GLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF
Sbjct: 423 IIINGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGF 482

Query: 421 GGRMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE 478
           GGRMCVGRHLTFMEYKIVLTLILSRFSFSLS DY+HSPSIMLSLRPAHGLPLVFRLLE+
Sbjct: 483 GGRMCVGRHLTFMEYKIVLTLILSRFSFSLSPDYRHSPSIMLSLRPAHGLPLVFRLLED 539

[Close]

BLAST of CSPI05G07210.1 vs. NCBI nr
Match: XP_038875746.1 (LOW QUALITY PROTEIN: cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 823.5 bits (2126), Expect = 9.1e-235
Identity = 417/477 (87.42%), Postives = 436/477 (91.40%), Query Frame = 0


Query: 1   MKRMNANNPNSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEP 60
           MK+ N  NPN T E+SS  HQ+THDIHS VFPYFARWQTTYGKVFTYWLGTEPFLYI EP
Sbjct: 59  MKKKNV-NPN-TFEESS--HQVTHDIHSIVFPYFARWQTTYGKVFTYWLGTEPFLYIGEP 118

Query: 61  ELVKILSKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAM 120
           E+VKILSKEVKAK+WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAM
Sbjct: 119 EIVKILSKEVKAKYWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAM 178

Query: 121 ASLMVESATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLR 180
           ASLM ESATQMVERWANL+ISGNP IEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLR
Sbjct: 179 ASLMAESATQMVERWANLVISGNPXIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLR 238

Query: 181 QLQFTLFKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTE 240
            LQ TLFKTNRLVGVPFAG+LNAAKAR+AK LG+EID LF SVI ERRENA A      E
Sbjct: 239 HLQTTLFKTNRLVGVPFAGVLNAAKARQAKRLGKEIDALFMSVIKERRENAAA---AAGE 298

Query: 241 QQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVH 300
           QQNDLLSLLLKE        GE R LST ELIDECKTFFFGGHETTALALTWTLMLLA+H
Sbjct: 299 QQNDLLSLLLKE--------GE-RALSTTELIDECKTFFFGGHETTALALTWTLMLLAIH 358

Query: 301 SEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIT 360
           SEWQT LREEIKEVFG N+QT FDFTNL+SLKKMGWVMSEVLRLYPSAPNVQRQARKDIT
Sbjct: 359 SEWQTKLREEIKEVFGDNIQTHFDFTNLASLKKMGWVMSEVLRLYPSAPNVQRQARKDIT 418

Query: 361 IDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGG 420
           I+GLTIPNGTN+WIDVV+MHHDQALWGHQVNEFHPERF+ND VSGGCTHKMGYLPFGFGG
Sbjct: 419 INGLTIPNGTNIWIDVVAMHHDQALWGHQVNEFHPERFQNDVVSGGCTHKMGYLPFGFGG 478

Query: 421 RMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLEE 478
           RMCVGRHLTFMEYKIVLT ILSRFSFSLSSDY+HSPSIMLSLRP HGLPLVFRLL++
Sbjct: 479 RMCVGRHLTFMEYKIVLTHILSRFSFSLSSDYRHSPSIMLSLRPIHGLPLVFRLLDD 519

[Close]

BLAST of CSPI05G07210.1 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 555.4 bits (1430), Expect = 4.3e-158
Identity = 273/463 (58.96%), Postives = 338/463 (73.00%), Query Frame = 0


Query: 13  VEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEVKA 72
           V++S S   + HDIHS   P+FARWQ  YGKVF YWLG EPF+Y+A+PE + ++SK V  
Sbjct: 73  VDNSKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSKGVLG 132

Query: 73  KHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVESATQMV 132
           K WGKP+VFK DR+ MFG GLVM EGD+W R RHIITPAF P NLK M ++MVES + M+
Sbjct: 133 KSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESVSNML 192

Query: 133 ERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQFTLFKTNRL 192
           +RW   I SGNP+ ++E EI  TAGEIIAKTSFG+  E+G QV   LR +QF LF +NR 
Sbjct: 193 DRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQFALFNSNRY 252

Query: 193 VGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQNDLLSLLLKE 252
           VGVPF+ +L+  +  +AK LG EID L  S I  +R+ ++AE     +Q +DLL +LLK 
Sbjct: 253 VGVPFSNILSYKQTVKAKGLGHEIDGLLLSFI-NKRKISLAE---GDDQGHDLLGMLLKA 312

Query: 253 SSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTILREEIK 312
              G          +  EL+DECKTFFF GHETTALALTWT MLLA+H EWQ  +REEI+
Sbjct: 313 DQKG--------NFTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIREEIR 372

Query: 313 EVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDGLTIPNGTNM 372
           EV G    ++ ++  L+ LKKM WVM+EVLRLYP APN QRQAR DI ++G  IPNGTN+
Sbjct: 373 EVIG---DSKIEYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTNI 432

Query: 373 WIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGRHLTFME 432
           WIDVV+MHHD  LWG  VNEF PERF +  + GGC +KMGY+PFGFGGRMC+GR+LT ME
Sbjct: 433 WIDVVAMHHDVELWGDDVNEFKPERF-DGNLHGGCKNKMGYMPFGFGGRMCIGRNLTTME 492

Query: 433 YKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLL 476
           YKIVL+L+LSRF  S+S  Y+HSP+ MLSLRP +GLPL+ R L
Sbjct: 493 YKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

[Close]

BLAST of CSPI05G07210.1 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 318.5 bits (815), Expect = 8.9e-87
Identity = 177/468 (37.82%), Postives = 265/468 (56.62%), Query Frame = 0


Query: 11  STVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEV 70
           S  +D  S H   HDI   + P++  W   YGK F  W GT+P L + E EL+    KE+
Sbjct: 66  SASKDCDSIH---HDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELI----KEL 125

Query: 71  KAKH---WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVES 130
             KH    G+  + +   K+  G GL+MA G +W  QRH+  PAF    LK  A  MVE 
Sbjct: 126 LMKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVEC 185

Query: 131 ATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQFTLF 190
            +++VER    +  G  ++E+ +E+     +II++T FG   E G+++FN L  LQ    
Sbjct: 186 TSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCA 245

Query: 191 KTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQNDLLS 250
           +  R +  P +  L +   RE K L +E++ L   +I  RR+   AE  R++   +DLL 
Sbjct: 246 QATRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRD--CAEMGRSSTHGDDLLG 305

Query: 251 LLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTIL 310
           LLL E             L    ++DECKTFFF GHETTAL LTWT MLLA +  WQ  +
Sbjct: 306 LLLNEMDIDKNNNNNNNNLQL--IMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKV 365

Query: 311 REEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDGLTIP 370
           REE++EVFG N     D   LS L  +  V++E LRLYP A  + R A +D+ +  LTIP
Sbjct: 366 REEVREVFGRNGLPSVD--QLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIP 425

Query: 371 NGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGRH 430
            G ++WI V+++HH + LWG   N+F+PERF     + G      ++PF  G R C+G+ 
Sbjct: 426 KGLSIWIPVLAIHHSEELWGKDANQFNPERFGGRPFASG----RHFIPFAAGPRNCIGQQ 485

Query: 431 LTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLL 476
              ME KI+L  ++S+F+F++S +Y+H+P ++L+++P +G+ ++ + L
Sbjct: 486 FALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

[Close]

BLAST of CSPI05G07210.1 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 302.0 bits (772), Expect = 8.6e-82
Identity = 172/470 (36.60%), Postives = 263/470 (55.96%), Query Frame = 0


Query: 10  NSTVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKE 69
           +S   D SS H   H+I   + P++  W   YGK F  W GTEP L + E E++    KE
Sbjct: 66  HSASNDCSSIH---HNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMI----KE 125

Query: 70  VKAKH---WGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVE 129
           +  KH    GK  + +   K   G GL+MA G+ W  QRH+  PAF    LK  A  MVE
Sbjct: 126 LLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVE 185

Query: 130 SATQMVERWANLIISGNPQIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQFTL 189
               M ER    +     ++E+ +E+     +II++T FG   + G+++F+ L  LQ   
Sbjct: 186 CTKMMAERLRKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLC 245

Query: 190 FKTNRLVGVPFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQNDLL 249
            +  R +  P +  L +   RE K L  E++ L   +I  R+++   E  R++   +DLL
Sbjct: 246 AQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDS--VEIGRSSSYGDDLL 305

Query: 250 SLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVHSEWQTI 309
            LLL +  S          L+   ++DECKTFFF GHETT+L LTWTLMLLA +  WQ  
Sbjct: 306 GLLLNQMDS------NKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDN 365

Query: 310 LREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDITIDGLTI 369
           +R+E+++V G +     +   LSSL  +  V++E LRLYP A  + R A +DI +  L I
Sbjct: 366 VRDEVRQVCGQDGVPSVE--QLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLII 425

Query: 370 PNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGGRMCVGR 429
           P G ++WI V+++HH   LWG   NEF+PERF   + +        ++PF  G R C+G+
Sbjct: 426 PKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSFAS----SRHFMPFAAGPRNCIGQ 485

Query: 430 HLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLLE 477
               ME KI+L +++S+FSF++S +Y+H+P ++L+++P +G+ LV + L+
Sbjct: 486 TFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

[Close]

BLAST of CSPI05G07210.1 vs. TAIR 10
Match: AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )

HSP 1 Score: 296.2 bits (757), Expect = 4.7e-80
Identity = 171/482 (35.48%), Postives = 272/482 (56.43%), Query Frame = 0


Query: 11  STVEDSSSHHQ----LTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKIL 70
           S +  +S H+     + HD  S++FPY   W+  YG+V+TY  G +  LY+  PELVK L
Sbjct: 67  SQIMSNSKHYSGDNIIAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKEL 126

Query: 71  SKEVKAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVE 130
           + +    + GK S      KS+ G G++ + G  W  QR II P F    +K M  L+VE
Sbjct: 127 N-QANTLNLGKVSYVTKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVE 186

Query: 131 SATQMVERWANLIISGNP---QIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQ 190
           SA  M+ +W  ++         I V++++ + + ++I++  FG     G+++F+KLR LQ
Sbjct: 187 SAMPMLSKWEEMMKREGEMVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQ 246

Query: 191 FTLFKTNRLVGV-PFAGLLNAAKAREAKVLGEEIDELFRSVIAERRENAVAETTRTTEQQ 250
             +   N L  +  F  ++   K    K    +IDEL R +     E+ + ET +  E++
Sbjct: 247 KAITHNNILFSLNGFTDVVFGTK----KHGNGKIDELERHI-----ESLIWETVKERERE 306

Query: 251 ------NDLLSLLLKESSSGGGGGGEGRRLS-TAELIDECKTFFFGGHETTALALTWTLM 310
                  DL+ L+L+ + S   G  E +  S  + ++D CK+ +F GHET+A+A++W LM
Sbjct: 307 CVGDHKKDLMQLILEGARSSCDGNLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLM 366

Query: 311 LLAVHSEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQA 370
           LLA++  WQT +R+   EVF H      D  ++S+LK +  V+ E LRLYP A  V R+A
Sbjct: 367 LLALNPSWQTRIRD---EVFLHCKNGIPDADSISNLKTVTMVIQETLRLYPPAAFVSREA 426

Query: 371 RKDITIDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLP 430
            +D  +  L +P G  +W  + ++H D  +WG   NEF+PERF ++ VS  C H   ++P
Sbjct: 427 LEDTKLGNLVVPKGVCIWTLIPTLHRDPEIWGADANEFNPERF-SEGVSKACKHPQSFVP 486

Query: 431 FGFGGRMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGLPLVFRLL 478
           FG G R+C+G++   ME K++++LI+SRFSF+LS  YQHSP   + + P HG  +V R+L
Sbjct: 487 FGLGTRLCLGKNFGMMELKVLVSLIVSRFSFTLSPTYQHSPVFRMLVEPQHG--VVIRVL 532

[Close]

BLAST of CSPI05G07210.1 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 282.3 bits (721), Expect = 7.1e-76
Identity = 157/468 (33.55%), Postives = 257/468 (54.91%), Query Frame = 0


Query: 11  STVEDSSSHHQLTHDIHSTVFPYFARWQTTYGKVFTYWLGTEPFLYIAEPELVKILSKEV 70
           S  +  S  + ++HD  S++FP+F  W+  YG+++TY  G +  LYI  PE+VK LS + 
Sbjct: 67  SEAKHCSGDNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELS-QT 126

Query: 71  KAKHWGKPSVFKNDRKSMFGNGLVMAEGDEWVRQRHIITPAFNPSNLKAMASLMVESATQ 130
              + G+ +        + GNG++ + G  W  QR II   F    +K M  LMVESA  
Sbjct: 127 NTLNLGRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMP 186

Query: 131 MVERWANLIISGNP---QIEVEQEITSTAGEIIAKTSFGIHHESGRQVFNKLRQLQFTLF 190
           M+ +W  ++  G      I V++++   + ++IAK  FG     G+ +F+ +R L   + 
Sbjct: 187 MLNKWEEMVKRGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAI- 246

Query: 191 KTNRLVGVPFAGLLNAAKAREAKVLGE-EIDELFRSVIAERRENAVAETTRTTE------ 250
            T R V   F G  +      +K  G+ +ID L   +     E+++ ET +  E      
Sbjct: 247 -TKRSVLFRFNGFTDMVFG--SKKHGDVDIDALEMEL-----ESSIWETVKEREIECKDT 306

Query: 251 QQNDLLSLLLKESSSGGGGGGEGRRLSTAELIDECKTFFFGGHETTALALTWTLMLLAVH 310
            + DL+ L+L+ +     G    +      ++D CK+ +F GH++TA++++W LMLLA++
Sbjct: 307 HKKDLMQLILEGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALN 366

Query: 311 SEWQTILREEIKEVFGHNLQTQFDFTNLSSLKKMGWVMSEVLRLYPSAPNVQRQARKDIT 370
             WQ  +R+EI     + +    D  ++ +LK +  V+ E +RLYP AP V R+A KDI 
Sbjct: 367 PSWQVKIRDEILSSCKNGIP---DAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIR 426

Query: 371 IDGLTIPNGTNMWIDVVSMHHDQALWGHQVNEFHPERFRNDTVSGGCTHKMGYLPFGFGG 430
           +  L +P G  +W  + ++H D  +WG   N+F PERF ++ +S  C +   Y+PFG G 
Sbjct: 427 LGDLVVPKGVCIWTLIPALHRDPEIWGPDANDFKPERF-SEGISKACKYPQSYIPFGLGP 486

Query: 431 RMCVGRHLTFMEYKIVLTLILSRFSFSLSSDYQHSPSIMLSLRPAHGL 469
           R CVG++   ME K++++LI+S+FSF+LS  YQHSPS  L + P HG+
Sbjct: 487 RTCVGKNFGMMEVKVLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGV 520

The following BLAST results are available for this feature:

BLAST of CSPI05G07210.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FF18	1.2e-85	37.82	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1	[more]
Q9ZW95	1.2e-80	36.60	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1	[more]
Q93Z79	6.6e-79	35.48	Cytochrome P450 714A1 OS=Arabidopsis thaliana OX=3702 GN=CYP714A1 PE=2 SV=1	[more]
Q2QYH7	5.6e-78	33.82	Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ...	[more]
Q5KQH7	2.6e-75	33.91	Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 ...	[more]

BLAST of CSPI05G07210.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKS5	9.7e-275	99.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166390 PE=3 SV=1	[more]
E5GCU7	1.6e-256	93.53	Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A1S4DS94	1.6e-256	93.53	cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482547 PE=3 SV=1	[more]
A0A6J1H0W5	1.9e-214	82.02	cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459387 PE=3 SV...	[more]
A0A6J1K6M8	4.3e-214	81.32	cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491149 PE=3 SV=1	[more]

BLAST of CSPI05G07210.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654827.1	3.7e-276	99.79	cytokinin hydroxylase [Cucumis sativus]	[more]
KAE8647911.1	3.7e-276	99.79	hypothetical protein Csa_000052 [Cucumis sativus]	[more]
ADN34296.1	3.2e-256	93.53	cytochrome p450 [Cucumis melo subsp. melo]	[more]
XP_016898838.1	3.2e-256	93.53	PREDICTED: cytokinin hydroxylase-like [Cucumis melo]	[more]
XP_038875746.1	9.1e-235	87.42	LOW QUALITY PROTEIN: cytokinin hydroxylase-like [Benincasa hispida]	[more]

BLAST of CSPI05G07210.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52400.1	4.3e-158	58.96	cytochrome P450, family 715, subfamily A, polypeptide 1	[more]
AT5G38450.1	8.9e-87	37.82	cytochrome P450, family 735, subfamily A, polypeptide 1	[more]
AT1G67110.1	8.6e-82	36.60	cytochrome P450, family 735, subfamily A, polypeptide 2	[more]
AT5G24910.1	4.7e-80	35.48	cytochrome P450, family 714, subfamily A, polypeptide 1	[more]
AT5G24900.1	7.1e-76	33.55	cytochrome P450, family 714, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 423..446 score: 32.68 coord: 290..316 score: 32.66 coord: 38..57 score: 30.39 coord: 413..423 score: 51.95 coord: 270..287 score: 27.5
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 423..434 score: 34.73 coord: 336..347 score: 39.82 coord: 414..423 score: 57.77 coord: 281..298 score: 39.8
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 27..456 e-value: 1.6E-81 score: 274.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 16..475 e-value: 6.4E-112 score: 376.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 25..473
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..17
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 10..471
None	No IPR available	PANTHER	PTHR24282:SF149	CYTOCHROME P450 FAMILY 72 PROTEIN	coord: 10..471
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 416..425

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI05G07210	CSPI05G07210	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI05G07210.1.cds1	CSPI05G07210.1.cds1	CDS
CSPI05G07210.1.cds2	CSPI05G07210.1.cds2	CDS
CSPI05G07210.1.cds3	CSPI05G07210.1.cds3	CDS
CSPI05G07210.1.cds4	CSPI05G07210.1.cds4	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI05G07210.1	CSPI05G07210.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen