CSPI03G28360 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI03G28360
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionMono-/di-acylglycerol lipase, N-terminal;Lipase, class 3
LocationChr3: 25944630 .. 25949882 (-)
RNA-Seq ExpressionCSPI03G28360
SyntenyCSPI03G28360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGAAAGTCGGTTTGATTGCGTTCGTGTAATCTTGGGAAAAATAGGAAGAAAGAATTGGCGCGTGTAATTGACAAATTATAGTTACGAAACTCTAACCCCTAAACAAAGTTCCATTCTCTCTTTTGCTTCGTTGCGAATCAATTAAAACTCAACTGCTTTCCCCAAGCAGCGATCGAAACCCCCAAAACCTCATAACTCTTCTCCACACTATAAATTCCGAAATCCTTCCCAACCCCAACCCCCCATTCTTTTCTTACGATTTCCGGCCACCATCGTTGTTTTTCGGGTACGCATTCTCCCTTCATTGTTCTTCAATTTTGCTGATTCATTCCTTAATTCCCGATCCTTTTTGTGTTCTTTGTTTCGTACTGGACATTTCTGTAATTGAACTCTCCTCATATATGCTTTTTATGGTAGAATTTCATATATCTTCACGGTTTTTTGTTTCGGTTGCGTCGATAATATGGAAAGAGAAGATACCGTGTTCTTGGCTATTCATTTCTGTTGCACCCTTTGGAAATTTTACTCCCACTGGATTGTAATTATTGTAACAAAGAAGAATTGAAGTTGAAAACCCCTTGCATGTATGTGTCAAGGGATTGAGAAGTTTCTGAAATTTTGGGGTTTTTCGTCTTGAAACGCGTTCTTTTACCTGCTTTCTTGCTCTAGGGGATACAAGTTCATGGCAACTGCAACTATGGCCACGGCTGCTGGTGCAGCTGCACTTCTTTATTATACATTGAACCGTAAGTTGCACTCAAGTGGAGATCAAGATGATGGGGATGTAGATGGTAATGATGCTTCCACTCATGCTCTTCTTGGAGGTGATCGAGTTTCTCATAGACTGATTCAAGCGCCTGCTACATGGCTTGAGACAATTTCAACATTATCTGAGACCTTAAGGTTTACATACTCGGAGACTCTTGGAAAGTGGCCCATTGGGGATTTGGCATTTGGAATTAATTTTCTCTTAAAGAGACAGGTATGGAGCTTTTTGATGAATTAATAATGTGTAGTCTATTGTATGTTTTAAGCTGGTTGGTTCTTGCTTTCACTATGTCTCCGGTCGAAACTCAAGGTACTGATATTAATTTGTTTCCGTTAGGGAGTTTTATTCATCTTTCACGTTGGGTTACGTGATGTAGTGGTCTTCATATCATTTGATCTCTCATTCTGCTAACGTATGTAAAATTTTGTATGATGAGTCATTATGCTCCTGTAAGAATTTTATTGTTGTTTTACCTTTCATACATTTACTCAGAGGTAAAGAAATGTCTAAACTCTTCTATTAGTTTTCTATAAACAGAAAAAATTGTAATTATGTTATTCATATGTATTTATGGAAGAAGAATAGAGGTGAGCTAATTATATTATCATTGCATATCAAGGGGCTTAGAGTTCTTGGTTTTGGCGGAAAAGAGAGGATGGTTATCCATTTTCACTTGGAAAGACTTATTGTATGGTGCTTGGCAAGCCCTACTTTATTGTTGGGTTTGGATAAAGATGACTTTTGAAAAAAGGGGAGTTTTAACTTCTCCAAATTAAGATTTCTTTCTTAAAAACTTTGACGTTTCTTCTAGTGTGTAATGGGTATGGTGGATTATTGAGGAACTTCAAGTTTTTATCTTGTATCAAATAATAGGATTATTCACTAACGTTAGTCTGGTTTTTATTGGTAATTTATAAGATACAAATTGTGAAGGAAGATAAAAATGGTTATTCCATATGAAATTCTTGATTTAGATGTAGTTTGTGGAATTGGGAGGTTCTTTTAGCGTTAATGTAGTAGTTCTAAAGGGGATGATATTGTAATTCTTTTTCAGGGATTTTTTTCGAGTACAATGTTGTGATTTTAATACTTTAAAGGAAGATTTTTTTTCTTCACTACAATTTTTCACACGTCAATCGAAAACACTTGCTTTCTTTGAATAGAAAAAAAAATCCTCAATAGAAGAAGTTAATGTTTTATCTGAACAATCTAAATTAATTAATTTCTAGTGCTGCTACATTCTTTTCGGTTTGGCCTTTGATAGAATGTGCTTATTTGTGCAGGGGAACTTACATGTAGGCAGCGTATTTGGTAATGAAGACAGTATTCAGCTAAAGGGAACGGAAATGATTACTGAGCTGAAGTACCTCTTGCACTTATTGACTTTGTGTTGGCATTTCTCAAAAAAACCATTTCCGCTGTTTCTAGAAGAAACTGGTTTTTCTAAGGAAAATGTTCTCCTTCAGGAACCAAAAGCAGGAGTAAGTCTTGATGAATTTCAGATAATTGAAATAGATATTGTACAGTATTTGCTCTCCCACTCATTTTTGTTACATTTATTATCCACATTTATCACATTTATCCATGAACTGCAGATTTTGAAGCCTGCTTTTACCATTTTAGTTGACCATAATACAAAATGTATTCTCCTGTTGATTCGTGGAACACACAGTATCAAAGACACTCTTACAGCTGCCACTGGAGCTGTGGTGCCATTCCATCACAGTGTCGTGCATGAAGGAGGGGTTAGCAATTTGGTTTTAGGATATGCGCACTGTGGAATGGTTGCAGCTGCTCGTTGGATTGCAAAGCTGTCTACTCCTTGTCTTTTGAAAGCTCTTGGTCAATATTCTGGTTATAATATTAAGGTTGGATATTTCTTCTTAGTTTACCATGATACTTTTTAACAGATAAAAAGTAGACAAATGAAAAAGATCGATGGAGTAATCATGTGTTGGTTTCTAATAGGTCGTGGGGCATTCTTTGGGTGGAGGAACAGCTGCACTTCTAACTTATATTTTAAGAGAGCAGAAGGAGTTATCAATTACTTCTTGTGTTACATTTGCCCCAGGTCTGTATTAGTTTTGGATGGTTTCTCTGTGGTTTTTTTATTATGCCCATTATACATGACATGATGTTTGGACTTCTCATTTTTAATGAATCGATCATGTTACTAATTAGCTTTAATCTCTGCAGCTGCTTGTATGACATGGGAACTAGCAGAGTCAGGCAATGAATTTATCACTTCTGTGATTAATGGAGCTGATTTGGTGCCCACCTTCTCAGCTGCTTCAGTAGATGACCTGCGCGGTGAGGTATGTTTCAAGTTCATCTGATTATTGAGCAATATATTTGCTTCACGTTACTACTTTATGAATTATGTGTCTTTTTTTCCTGAAAAATTTCAAATAATATTTCATCTTTTGGTGAATAAATTTTTAAGTTGTTAGAGTTTAAGAATGAATGTGTGTGGATCGATTGTGCCTAACCCATCCTTTTCAATAAATAATAACGAATTAAAATATGTGCCTAATCTGTTTGTATACATTTTGCCTGATAGAGTTTACTTGGCTTTTTATATATACTATGGAAAAGGTTGTCTGTTAAGGCATATGAAGTTAGAATATATGGGCACGTGTGTGTTCTACTCACCAAGATGAATGAAGTTTAGATAAACACATTTAGTTAACTTGACAACATTCCTCTGAAATTGTGCTCAAGCCATTGAAATTTCTGGTATAGGTTACTGCTTCTGCTTGGGTAAATGATCTGAGAAATCAAATTGAGCGCACTCGAATTCTTAGCACTGTTTACCGTTCTGCTTCAGCATTGGGATCCCGTCTCCCATCCATAGCGAGTGCAAGAGCAAAAGTCGCTGGTGCAGGAGCCATTCTGAGACCAGTCTCCAGTGGCACACAGGTAACATGCGTTGTGCATATTAATAATTTTAGGAGAGCTGAAATTTGATAGAAATTTCGGAATGCACGAGATCTGGTTTGAATTCTTAATAGTTCTCTGAAATGTAGGCTTAGGACCTTGTGATCATCATTTAGACTTGAAATAGACAGGTCATCCAATGCAAATAAGATGCCTTTTGTTGTGGAGTTATCTTGGATTCCCATGAAAATGGATACCGGAGGGGTTTTTTATTTTATTTTGATTTATTTTCCTTCTACTATAACGTGGAATACACGAACAGGCATCTTAGCATGTTTGTACTATTTAAAAACTGTAGAAATACTTCAACCACTTTATTATATAAAGAGCTTAGTAACAAAAGTTTAGCCAATAGTTAGTTAACAGTGAAATCAATACTAAAATCAGTTTAGTACACCTGATACATAACACAAACACAATCATCTTAAGCTATGAGACATCCAAATTCTTGAACAGTTAGACACAGTTACATACCGGACATCTAAAGATACTTAATATTCTTTGGGGGGTTTTATGGGAGCTTAGTTCCTGTGACTTGTATATAATATTTAAATTTATACCTTGTTGTGTTTTGTATTTCTGTAGGTTGTGATGAAGAGAGCGCAGAGCATGGCTCAGGCAGCATGGACACGACCTTCACTCCATTTATCATCTTGGTCATGCATAGGCCCACGTCGTAGAGCCATGGCTTCTCACTCAGTGGCTGAAGAAGGTGGAAGTTCTCCAAAACCATCTCCAAGAAAAATGGAATCTTGTGAGCCACTTAGATCATCCCCGGAAGAAACTGTCGAAGCTATTGAAGCTATTGAACCTCCTGAATCTTCCACTACGGCGATGCAATGGAGTAATGAAATTGAATATTCCTATTCTGAGGAGATAAATCCTGAGGGTATCACAGATGAGCTTGAGGATGATGGTCAAACCCTCATGGGCAATATTCAAGATGAACAAATGACGGAAGTTGAATTATGGCAGCAACTCGAGCATGAACTATACGATAAGGGCGAACCTGATGTTGCGGAGGAGATAAGAGAAGAAGAAGCTGCTGCTATGGCAGAAGTAGGACAGTCTGATAGCTCTGCTTGTGGAATTAAGGAAGCGCACAGATTTTTTCCCGCTGGGAAGATCATGCATATGATCGATATCCAATCAGATGCCCCTGATTGTGAAAGTGATAGCAGCAGCTCCAGATCCAGCATCTCAGAAAATAGCCCCCTGGCGGAGTGTAAGATCGGTATTTTCCTTACATCAAGATCATTGTATAGCAAACTCAGATTATCTCAGACAATGATAAGTGACCACTACATGCCAGCTTATAGAAGACAGATAGAAAAGTTAATTAAAGAATTGGAGAAAGAAGAATGTTTTAATCTTGGAATGGAAAGGTAGAGTTCTATAGAGAAATGTGAACATTTTATTAAAGAACTTGTGTACAGAATAGGATTTGCAGCCCCCAATTGATTTAGAAAACGGATTTTCTTATAGCGCAAAAGTTTTCCACTACTGTGGTTGAATGAGGTAATTACTAATTACTTGTTGGCAGAGC

mRNA sequence

TGGAAAGTCGGTTTGATTGCGTTCGTGTAATCTTGGGAAAAATAGGAAGAAAGAATTGGCGCGTGTAATTGACAAATTATAGTTACGAAACTCTAACCCCTAAACAAAGTTCCATTCTCTCTTTTGCTTCGTTGCGAATCAATTAAAACTCAACTGCTTTCCCCAAGCAGCGATCGAAACCCCCAAAACCTCATAACTCTTCTCCACACTATAAATTCCGAAATCCTTCCCAACCCCAACCCCCCATTCTTTTCTTACGATTTCCGGCCACCATCGTTGTTTTTCGGGGGATACAAGTTCATGGCAACTGCAACTATGGCCACGGCTGCTGGTGCAGCTGCACTTCTTTATTATACATTGAACCGTAAGTTGCACTCAAGTGGAGATCAAGATGATGGGGATGTAGATGGTAATGATGCTTCCACTCATGCTCTTCTTGGAGGTGATCGAGTTTCTCATAGACTGATTCAAGCGCCTGCTACATGGCTTGAGACAATTTCAACATTATCTGAGACCTTAAGGTTTACATACTCGGAGACTCTTGGAAAGTGGCCCATTGGGGATTTGGCATTTGGAATTAATTTTCTCTTAAAGAGACAGGGGAACTTACATGTAGGCAGCGTATTTGGTAATGAAGACAGTATTCAGCTAAAGGGAACGGAAATGATTACTGAGCTGAAGTACCTCTTGCACTTATTGACTTTGTGTTGGCATTTCTCAAAAAAACCATTTCCGCTGTTTCTAGAAGAAACTGGTTTTTCTAAGGAAAATGTTCTCCTTCAGGAACCAAAAGCAGGAATTTTGAAGCCTGCTTTTACCATTTTAGTTGACCATAATACAAAATGTATTCTCCTGTTGATTCGTGGAACACACAGTATCAAAGACACTCTTACAGCTGCCACTGGAGCTGTGGTGCCATTCCATCACAGTGTCGTGCATGAAGGAGGGGTTAGCAATTTGGTTTTAGGATATGCGCACTGTGGAATGGTTGCAGCTGCTCGTTGGATTGCAAAGCTGTCTACTCCTTGTCTTTTGAAAGCTCTTGGTCAATATTCTGGTTATAATATTAAGGTCGTGGGGCATTCTTTGGGTGGAGGAACAGCTGCACTTCTAACTTATATTTTAAGAGAGCAGAAGGAGTTATCAATTACTTCTTGTGTTACATTTGCCCCAGCTGCTTGTATGACATGGGAACTAGCAGAGTCAGGCAATGAATTTATCACTTCTGTGATTAATGGAGCTGATTTGGTGCCCACCTTCTCAGCTGCTTCAGTAGATGACCTGCGCGGTGAGGTTACTGCTTCTGCTTGGGTAAATGATCTGAGAAATCAAATTGAGCGCACTCGAATTCTTAGCACTGTTTACCGTTCTGCTTCAGCATTGGGATCCCGTCTCCCATCCATAGCGAGTGCAAGAGCAAAAGTCGCTGGTGCAGGAGCCATTCTGAGACCAGTCTCCAGTGGCACACAGGTTGTGATGAAGAGAGCGCAGAGCATGGCTCAGGCAGCATGGACACGACCTTCACTCCATTTATCATCTTGGTCATGCATAGGCCCACGTCGTAGAGCCATGGCTTCTCACTCAGTGGCTGAAGAAGGTGGAAGTTCTCCAAAACCATCTCCAAGAAAAATGGAATCTTGTGAGCCACTTAGATCATCCCCGGAAGAAACTGTCGAAGCTATTGAAGCTATTGAACCTCCTGAATCTTCCACTACGGCGATGCAATGGAGTAATGAAATTGAATATTCCTATTCTGAGGAGATAAATCCTGAGGGTATCACAGATGAGCTTGAGGATGATGGTCAAACCCTCATGGGCAATATTCAAGATGAACAAATGACGGAAGTTGAATTATGGCAGCAACTCGAGCATGAACTATACGATAAGGGCGAACCTGATGTTGCGGAGGAGATAAGAGAAGAAGAAGCTGCTGCTATGGCAGAAGTAGGACAGTCTGATAGCTCTGCTTGTGGAATTAAGGAAGCGCACAGATTTTTTCCCGCTGGGAAGATCATGCATATGATCGATATCCAATCAGATGCCCCTGATTGTGAAAGTGATAGCAGCAGCTCCAGATCCAGCATCTCAGAAAATAGCCCCCTGGCGGAGTGTAAGATCGGTATTTTCCTTACATCAAGATCATTGTATAGCAAACTCAGATTATCTCAGACAATGATAAGTGACCACTACATGCCAGCTTATAGAAGACAGATAGAAAAGTTAATTAAAGAATTGGAGAAAGAAGAATGTTTTAATCTTGGAATGGAAAGGTAGAGTTCTATAGAGAAATGTGAACATTTTATTAAAGAACTTGTGTACAGAATAGGATTTGCAGCCCCCAATTGATTTAGAAAACGGATTTTCTTATAGCGCAAAAGTTTTCCACTACTGTGGTTGAATGAGGTAATTACTAATTACTTGTTGGCAGAGC

Coding sequence (CDS)

ATGGCAACTGCAACTATGGCCACGGCTGCTGGTGCAGCTGCACTTCTTTATTATACATTGAACCGTAAGTTGCACTCAAGTGGAGATCAAGATGATGGGGATGTAGATGGTAATGATGCTTCCACTCATGCTCTTCTTGGAGGTGATCGAGTTTCTCATAGACTGATTCAAGCGCCTGCTACATGGCTTGAGACAATTTCAACATTATCTGAGACCTTAAGGTTTACATACTCGGAGACTCTTGGAAAGTGGCCCATTGGGGATTTGGCATTTGGAATTAATTTTCTCTTAAAGAGACAGGGGAACTTACATGTAGGCAGCGTATTTGGTAATGAAGACAGTATTCAGCTAAAGGGAACGGAAATGATTACTGAGCTGAAGTACCTCTTGCACTTATTGACTTTGTGTTGGCATTTCTCAAAAAAACCATTTCCGCTGTTTCTAGAAGAAACTGGTTTTTCTAAGGAAAATGTTCTCCTTCAGGAACCAAAAGCAGGAATTTTGAAGCCTGCTTTTACCATTTTAGTTGACCATAATACAAAATGTATTCTCCTGTTGATTCGTGGAACACACAGTATCAAAGACACTCTTACAGCTGCCACTGGAGCTGTGGTGCCATTCCATCACAGTGTCGTGCATGAAGGAGGGGTTAGCAATTTGGTTTTAGGATATGCGCACTGTGGAATGGTTGCAGCTGCTCGTTGGATTGCAAAGCTGTCTACTCCTTGTCTTTTGAAAGCTCTTGGTCAATATTCTGGTTATAATATTAAGGTCGTGGGGCATTCTTTGGGTGGAGGAACAGCTGCACTTCTAACTTATATTTTAAGAGAGCAGAAGGAGTTATCAATTACTTCTTGTGTTACATTTGCCCCAGCTGCTTGTATGACATGGGAACTAGCAGAGTCAGGCAATGAATTTATCACTTCTGTGATTAATGGAGCTGATTTGGTGCCCACCTTCTCAGCTGCTTCAGTAGATGACCTGCGCGGTGAGGTTACTGCTTCTGCTTGGGTAAATGATCTGAGAAATCAAATTGAGCGCACTCGAATTCTTAGCACTGTTTACCGTTCTGCTTCAGCATTGGGATCCCGTCTCCCATCCATAGCGAGTGCAAGAGCAAAAGTCGCTGGTGCAGGAGCCATTCTGAGACCAGTCTCCAGTGGCACACAGGTTGTGATGAAGAGAGCGCAGAGCATGGCTCAGGCAGCATGGACACGACCTTCACTCCATTTATCATCTTGGTCATGCATAGGCCCACGTCGTAGAGCCATGGCTTCTCACTCAGTGGCTGAAGAAGGTGGAAGTTCTCCAAAACCATCTCCAAGAAAAATGGAATCTTGTGAGCCACTTAGATCATCCCCGGAAGAAACTGTCGAAGCTATTGAAGCTATTGAACCTCCTGAATCTTCCACTACGGCGATGCAATGGAGTAATGAAATTGAATATTCCTATTCTGAGGAGATAAATCCTGAGGGTATCACAGATGAGCTTGAGGATGATGGTCAAACCCTCATGGGCAATATTCAAGATGAACAAATGACGGAAGTTGAATTATGGCAGCAACTCGAGCATGAACTATACGATAAGGGCGAACCTGATGTTGCGGAGGAGATAAGAGAAGAAGAAGCTGCTGCTATGGCAGAAGTAGGACAGTCTGATAGCTCTGCTTGTGGAATTAAGGAAGCGCACAGATTTTTTCCCGCTGGGAAGATCATGCATATGATCGATATCCAATCAGATGCCCCTGATTGTGAAAGTGATAGCAGCAGCTCCAGATCCAGCATCTCAGAAAATAGCCCCCTGGCGGAGTGTAAGATCGGTATTTTCCTTACATCAAGATCATTGTATAGCAAACTCAGATTATCTCAGACAATGATAAGTGACCACTACATGCCAGCTTATAGAAGACAGATAGAAAAGTTAATTAAAGAATTGGAGAAAGAAGAATGTTTTAATCTTGGAATGGAAAGGTAG

Protein sequence

MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPATWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGTEMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNTKCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLSTPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELAESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASALGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPRRRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEIEYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIREEEAAAMAEVGQSDSSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSPLAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER*
Homology
BLAST of CSPI03G28360 vs. ExPASy Swiss-Prot
Match: Q9Y4D2 (Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3)

HSP 1 Score: 80.1 bits (196), Expect = 1.0e-13
Identity = 59/178 (33.15%), Postives = 88/178 (49.44%), Query Frame = 0

Query: 167 ILKPAFTILVDHNTKCILLLIRGTHSIKDTLTAATGAV----VPFHHSVVHEGGVSNLVL 226
           + +  F + VDH+ K +++ IRGT S KD LT  TG      V  HH             
Sbjct: 377 VYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHHGT----------- 436

Query: 227 GYAHCGMVAAARWIAKL--STPCLLKALGQYSG-----YNIKVVGHSLGGGTAALLTYIL 286
              H GMV +A +I K       L +A G+  G     Y + VVGHSLG GTAA+L+++L
Sbjct: 437 WLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLL 496

Query: 287 REQKELSITSCVTFA-PAACMTWELAESGNEFITSVINGADLVPTFSAASVDDLRGEV 333
           R Q       C  ++ P   ++ +  E   EF+T+V+ G DLVP    + ++  R ++
Sbjct: 497 RPQ--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQL 541

BLAST of CSPI03G28360 vs. ExPASy Swiss-Prot
Match: Q6WQJ1 (Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 3.0e-13
Identity = 59/176 (33.52%), Postives = 89/176 (50.57%), Query Frame = 0

Query: 167 ILKPAFTILVDHNTKCILLLIRGTHSIKDTLTAATGAV--VPFHHSVVHEGGVSNLVLGY 226
           + +  F + VDH+ K +++ IRGT S KD LT  TG    +P      H G         
Sbjct: 377 VYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEG---HRG------TWL 436

Query: 227 AHCGMVAAARWIAKL--STPCLLKALGQYSG-----YNIKVVGHSLGGGTAALLTYILRE 286
            H GMV +A +I K       L +A G+  G     Y + VVGHSLG GTAA+L+++LR 
Sbjct: 437 GHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRP 496

Query: 287 QKELSITSCVTFA-PAACMTWELAESGNEFITSVINGADLVPTFSAASVDDLRGEV 333
           Q       C  ++ P   ++ +  E   EF+T+V+ G DLVP    + ++  R ++
Sbjct: 497 Q--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQL 541

BLAST of CSPI03G28360 vs. ExPASy Swiss-Prot
Match: Q5YLM1 (Diacylglycerol lipase-alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.0e-13
Identity = 59/176 (33.52%), Postives = 89/176 (50.57%), Query Frame = 0

Query: 167 ILKPAFTILVDHNTKCILLLIRGTHSIKDTLTAATGAV--VPFHHSVVHEGGVSNLVLGY 226
           + +  F + VDH+ K +++ IRGT S KD LT  TG    +P      H G         
Sbjct: 377 VYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEG---HRG------TWL 436

Query: 227 AHCGMVAAARWIAKL--STPCLLKALGQYSG-----YNIKVVGHSLGGGTAALLTYILRE 286
            H GMV +A +I K       L +A G+  G     Y + VVGHSLG GTAA+L+++LR 
Sbjct: 437 GHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRP 496

Query: 287 QKELSITSCVTFA-PAACMTWELAESGNEFITSVINGADLVPTFSAASVDDLRGEV 333
           Q       C  ++ P   ++ +  E   EF+T+V+ G DLVP    + ++  R ++
Sbjct: 497 Q--YPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQL 541

BLAST of CSPI03G28360 vs. ExPASy Swiss-Prot
Match: P0C1S9 (Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.3e-12
Identity = 52/191 (27.23%), Postives = 92/191 (48.17%), Query Frame = 0

Query: 144 FPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNTKCILLLIRGTHSIKDTLTAATGA 203
           F   L+ TG    + +       + +  F +++DH  + +++ +RGT S++D LT  +  
Sbjct: 330 FASILKTTGLQYRDFIHVSFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAE 389

Query: 204 VVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAK--LSTPCLLKALGQYSGYNIKVVGH 263
                  +  +  V       AH G+  AAR+I +  ++   L +A      Y + VVGH
Sbjct: 390 SENLELDIELQDCV-------AHKGIAQAARYIYRRLVNDGILSQAFSVAPEYRLVVVGH 449

Query: 264 SLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELAESGNEFITSVINGADLVPTFS 323
           SLG G AALL  +LR      + +     P   ++  L E   +F+ S+I G D++P  S
Sbjct: 450 SLGAGAAALLAIMLRGAYP-QVRAYAFSPPRGLLSKSLFEYSKDFVVSLILGMDVIPRLS 509

Query: 324 AASVDDLRGEV 333
            A+++DL+  +
Sbjct: 510 VANMEDLKRRI 512

BLAST of CSPI03G28360 vs. ExPASy Swiss-Prot
Match: Q91WC9 (Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2)

HSP 1 Score: 72.8 bits (177), Expect = 1.6e-11
Identity = 53/191 (27.75%), Postives = 93/191 (48.69%), Query Frame = 0

Query: 144 FPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNTKCILLLIRGTHSIKDTLTAATGA 203
           F   L+ TG    + +       + +  F +++DH  + +++ +RGT S++D LT  +  
Sbjct: 330 FASILKTTGLQYRDFIHISFHDKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSA- 389

Query: 204 VVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAK--LSTPCLLKALGQYSGYNIKVVGH 263
                 S   E G+  L    AH G+  AAR+I +  ++   L +A      Y + +VGH
Sbjct: 390 -----ESETLELGI-ELQDCVAHKGIAQAARYIHRRLVNDGILSQAFSVAPEYQLVLVGH 449

Query: 264 SLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELAESGNEFITSVINGADLVPTFS 323
           SLG G AALL  +LR      + +     P   ++  L E   +F+ S+I G D++P  S
Sbjct: 450 SLGAGAAALLAIMLRGAYP-QVRAYAFSPPRGLLSKSLYEYSKDFVVSLILGMDVIPRLS 509

Query: 324 AASVDDLRGEV 333
             +++DL+  +
Sbjct: 510 VTNMEDLKRRI 512

BLAST of CSPI03G28360 vs. ExPASy TrEMBL
Match: A0A0A0LCM6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G644860 PE=4 SV=1)

HSP 1 Score: 1269.6 bits (3284), Expect = 0.0e+00
Identity = 655/657 (99.70%), Postives = 656/657 (99.85%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLR EVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRSEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480
           RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI
Sbjct: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480

Query: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540
           EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE
Sbjct: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540

Query: 541 EEAAAMAEVGQSDSSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600
           EEAAAMAEVGQSD+SACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP
Sbjct: 541 EEAAAMAEVGQSDTSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600

Query: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER 658
           LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER
Sbjct: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER 657

BLAST of CSPI03G28360 vs. ExPASy TrEMBL
Match: A0A1S3CC71 (uncharacterized protein LOC103499170 OS=Cucumis melo OX=3656 GN=LOC103499170 PE=4 SV=1)

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 636/657 (96.80%), Postives = 643/657 (97.87%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDA THALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDAPTHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKG 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGK 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTI+VDHNT
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMA AAWTRPSL LSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAAAAWTRPSLRLSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480
           RRAMASHSVAEEGGSSPKPSPRKMESCEPLRS+PEE V   EAIEP ESSTTAM+WSNEI
Sbjct: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSTPEEIV---EAIEPTESSTTAMEWSNEI 480

Query: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540
           EYSYSEEINPEGITDEL+DDGQTLM NIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE
Sbjct: 481 EYSYSEEINPEGITDELDDDGQTLMSNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540

Query: 541 EEAAAMAEVGQSDSSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600
           EEAAAMAEVGQSDSSA GIKEAHRFFPAGKIMH+IDIQSDAPDCESDSSSS SSISENSP
Sbjct: 541 EEAAAMAEVGQSDSSASGIKEAHRFFPAGKIMHIIDIQSDAPDCESDSSSSTSSISENSP 600

Query: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER 658
           LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQ+EKLIKELEKEEC+N  MER
Sbjct: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQVEKLIKELEKEECYNQIMER 654

BLAST of CSPI03G28360 vs. ExPASy TrEMBL
Match: A0A5A7UE47 (Mono-/di-acylglycerol lipase isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00080 PE=4 SV=1)

HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 620/638 (97.18%), Postives = 626/638 (98.12%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDA THALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDAPTHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKG 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGK 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTI+VDHNT
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMA AAWTRPSL LSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAAAAWTRPSLRLSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480
           RRAMASHSVAEEGGSSPKPSPRKMESCEPLRS+PEE V   EAIEP ESSTTAM+WSNEI
Sbjct: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSTPEEIV---EAIEPTESSTTAMEWSNEI 480

Query: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540
           EYSYSEEINPEGITDEL+DDGQTLM NIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE
Sbjct: 481 EYSYSEEINPEGITDELDDDGQTLMSNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540

Query: 541 EEAAAMAEVGQSDSSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600
           EEAAAMAEVGQSDSSA GIKEAHRFFPAGKIMH+IDIQSDAPDCESDSSSS SSISENSP
Sbjct: 541 EEAAAMAEVGQSDSSASGIKEAHRFFPAGKIMHIIDIQSDAPDCESDSSSSTSSISENSP 600

Query: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQI 639
           LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQ+
Sbjct: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQV 635

BLAST of CSPI03G28360 vs. ExPASy TrEMBL
Match: A0A6J1GYN5 (uncharacterized protein LOC111458044 OS=Cucurbita moschata OX=3662 GN=LOC111458044 PE=4 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 593/659 (89.98%), Postives = 619/659 (93.93%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLHSS DQDD DV GNDAS+HALLGGDRVSHRL+QAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSRDQDDSDVAGNDASSHALLGGDRVSHRLVQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHV  VFGNEDS QLKG 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVSGVFGNEDSSQLKGA 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           EMI+ELKYLLHLLTLCWHFSKKPFPLFLEETGFS+ENVLLQEPKAGILKPAFTI+VDHNT
Sbjct: 121 EMISELKYLLHLLTLCWHFSKKPFPLFLEETGFSEENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLL ALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELS+TSCVTFAPAACMTWELA
Sbjct: 241 TPCLLSALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSVTSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLR EVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSIASARAKVAGAGAIL+PVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILKPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480
           RRAM SHS AEE GSSPK SPRKMES EPLRSSP+E VEA E    PESSTT +QW+NEI
Sbjct: 421 RRAMTSHSTAEENGSSPKSSPRKMESFEPLRSSPQEIVEATEL---PESSTTTIQWTNEI 480

Query: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540
           E SYSEEINP+G+ DEL+DD Q LM +IQDEQ+TEVELWQQLEHEL+D+ E DVA+EIRE
Sbjct: 481 ECSYSEEINPDGMRDELDDDDQALMSHIQDEQITEVELWQQLEHELHDRSEADVAKEIRE 540

Query: 541 EEAAAMAEVGQSDSSACGIKEAHRFFPAGKIMHMIDI--QSDAPDCESDSSSSRSSISEN 600
           EEAAAMAEVGQSDS   G+KEAHRFFPAGKIMH+I+I  QSDAPDCESD SSSRSS S++
Sbjct: 541 EEAAAMAEVGQSDSFTSGMKEAHRFFPAGKIMHIIEIETQSDAPDCESD-SSSRSSTSDS 600

Query: 601 SPLAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER 658
           SP A+ + GIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKL+KELEKEEC N GMER
Sbjct: 601 SPQAQSRTGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLVKELEKEECCNRGMER 655

BLAST of CSPI03G28360 vs. ExPASy TrEMBL
Match: A0A6J1IRP8 (uncharacterized protein LOC111479893 OS=Cucurbita maxima OX=3661 GN=LOC111479893 PE=4 SV=1)

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 590/657 (89.80%), Postives = 617/657 (93.91%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLHSS DQDD DV GNDAS+HALLG DRVSHRL+QAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSRDQDDSDVAGNDASSHALLGVDRVSHRLVQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHV  VFGNEDS QLKG 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVSGVFGNEDSSQLKGA 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           EMI+ELKYLLHLLTLCWHFSKKPFPLFLEETGFS+ENVLLQEPKAGILKPAFTI+VDHNT
Sbjct: 121 EMISELKYLLHLLTLCWHFSKKPFPLFLEETGFSEENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLL ALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELS+TSCVTFAPAACMTWELA
Sbjct: 241 TPCLLSALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSVTSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLR EVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSIASARAKVAGAGAIL+PVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILKPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480
           RRAM SHS AEE GSSPK SPRKMES EPLRSSP+E +EA E    PESSTT +QW+NEI
Sbjct: 421 RRAMPSHSTAEENGSSPKSSPRKMESSEPLRSSPQEIIEATEL---PESSTTTIQWTNEI 480

Query: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540
           E SYSEEINP+G+ DEL+DD Q LM +IQDEQ+TEVELWQQLEHEL+D+ E DVA+EIRE
Sbjct: 481 ECSYSEEINPDGMRDELDDDDQALMSHIQDEQITEVELWQQLEHELHDRSEADVAKEIRE 540

Query: 541 EEAAAMAEVGQSDSSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600
           EEAAAMAEVGQSDS   G+KEAHRFFPAGKIMH+ID QSDAPDCESD SSSRSS S++SP
Sbjct: 541 EEAAAMAEVGQSDSFTSGMKEAHRFFPAGKIMHIIDTQSDAPDCESD-SSSRSSTSDSSP 600

Query: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER 658
            A+ +IGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKL++ELEKEEC N G ER
Sbjct: 601 QAQSRIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLVRELEKEECCNRGRER 653

BLAST of CSPI03G28360 vs. NCBI nr
Match: XP_004144431.1 (uncharacterized protein LOC101203983 [Cucumis sativus] >KGN58447.1 hypothetical protein Csa_002505 [Cucumis sativus])

HSP 1 Score: 1269.6 bits (3284), Expect = 0.0e+00
Identity = 655/657 (99.70%), Postives = 656/657 (99.85%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLR EVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRSEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480
           RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI
Sbjct: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480

Query: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540
           EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE
Sbjct: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540

Query: 541 EEAAAMAEVGQSDSSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600
           EEAAAMAEVGQSD+SACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP
Sbjct: 541 EEAAAMAEVGQSDTSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600

Query: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER 658
           LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER
Sbjct: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER 657

BLAST of CSPI03G28360 vs. NCBI nr
Match: XP_008460311.1 (PREDICTED: uncharacterized protein LOC103499170 [Cucumis melo])

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 636/657 (96.80%), Postives = 643/657 (97.87%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDA THALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDAPTHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKG 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGK 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTI+VDHNT
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMA AAWTRPSL LSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAAAAWTRPSLRLSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480
           RRAMASHSVAEEGGSSPKPSPRKMESCEPLRS+PEE V   EAIEP ESSTTAM+WSNEI
Sbjct: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSTPEEIV---EAIEPTESSTTAMEWSNEI 480

Query: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540
           EYSYSEEINPEGITDEL+DDGQTLM NIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE
Sbjct: 481 EYSYSEEINPEGITDELDDDGQTLMSNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540

Query: 541 EEAAAMAEVGQSDSSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600
           EEAAAMAEVGQSDSSA GIKEAHRFFPAGKIMH+IDIQSDAPDCESDSSSS SSISENSP
Sbjct: 541 EEAAAMAEVGQSDSSASGIKEAHRFFPAGKIMHIIDIQSDAPDCESDSSSSTSSISENSP 600

Query: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER 658
           LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQ+EKLIKELEKEEC+N  MER
Sbjct: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQVEKLIKELEKEECYNQIMER 654

BLAST of CSPI03G28360 vs. NCBI nr
Match: KAA0053494.1 (Mono-/di-acylglycerol lipase isoform 1 [Cucumis melo var. makuwa] >TYK19096.1 Mono-/di-acylglycerol lipase isoform 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 620/638 (97.18%), Postives = 626/638 (98.12%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDA THALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDAPTHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKG 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGK 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTI+VDHNT
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMA AAWTRPSL LSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAAAAWTRPSLRLSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480
           RRAMASHSVAEEGGSSPKPSPRKMESCEPLRS+PEE V   EAIEP ESSTTAM+WSNEI
Sbjct: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSTPEEIV---EAIEPTESSTTAMEWSNEI 480

Query: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540
           EYSYSEEINPEGITDEL+DDGQTLM NIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE
Sbjct: 481 EYSYSEEINPEGITDELDDDGQTLMSNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540

Query: 541 EEAAAMAEVGQSDSSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600
           EEAAAMAEVGQSDSSA GIKEAHRFFPAGKIMH+IDIQSDAPDCESDSSSS SSISENSP
Sbjct: 541 EEAAAMAEVGQSDSSASGIKEAHRFFPAGKIMHIIDIQSDAPDCESDSSSSTSSISENSP 600

Query: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQI 639
           LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQ+
Sbjct: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQV 635

BLAST of CSPI03G28360 vs. NCBI nr
Match: XP_038901142.1 (uncharacterized protein LOC120088127 isoform X1 [Benincasa hispida] >XP_038901147.1 uncharacterized protein LOC120088127 isoform X1 [Benincasa hispida])

HSP 1 Score: 1182.9 bits (3059), Expect = 0.0e+00
Identity = 618/657 (94.06%), Postives = 632/657 (96.19%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGND  +HALLGGDRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDTPSHALLGGDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTI+VDHNT
Sbjct: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLKAL QYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA
Sbjct: 241 TPCLLKALAQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSL LSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLRLSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480
           RRAMASHSVAEE GSSPKPSPRKME CEPLRSSPEE V   EAIE PESSTTAMQW+NEI
Sbjct: 421 RRAMASHSVAEEDGSSPKPSPRKMEPCEPLRSSPEEIV---EAIEHPESSTTAMQWTNEI 480

Query: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540
           E SYSEEI PEG+TD L+DDGQ LM +IQDEQMTEVELWQQLEHELYD+GEPDVA+EIRE
Sbjct: 481 ECSYSEEIIPEGMTDGLDDDGQALMDHIQDEQMTEVELWQQLEHELYDRGEPDVAKEIRE 540

Query: 541 EEAAAMAEVGQSDSSACGIKEAHRFFPAGKIMHMIDIQSDAPDCESDSSSSRSSISENSP 600
           EEAAAMA VGQSDSS  GIKEAHRFFPAGKIMH+IDIQSD+P CESDSSS+ SSIS+NSP
Sbjct: 541 EEAAAMAAVGQSDSSTSGIKEAHRFFPAGKIMHIIDIQSDSPVCESDSSSA-SSISDNSP 600

Query: 601 LAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER 658
           L E KIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKE+C+N  MER
Sbjct: 601 LEESKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEDCYNCRMER 653

BLAST of CSPI03G28360 vs. NCBI nr
Match: XP_022956309.1 (uncharacterized protein LOC111458044 [Cucurbita moschata])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 593/659 (89.98%), Postives = 619/659 (93.93%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKLHSS DQDD DV GNDAS+HALLGGDRVSHRL+QAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKLHSSRDQDDSDVAGNDASSHALLGGDRVSHRLVQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHV  VFGNEDS QLKG 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVSGVFGNEDSSQLKGA 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           EMI+ELKYLLHLLTLCWHFSKKPFPLFLEETGFS+ENVLLQEPKAGILKPAFTI+VDHNT
Sbjct: 121 EMISELKYLLHLLTLCWHFSKKPFPLFLEETGFSEENVLLQEPKAGILKPAFTIIVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLL ALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELS+TSCVTFAPAACMTWELA
Sbjct: 241 TPCLLSALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSVTSCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESGNEFITSVINGADLVPTFSAASVDDLR EVTASAWVNDLRNQIERTRILSTVYRSASA
Sbjct: 301 ESGNEFITSVINGADLVPTFSAASVDDLRAEVTASAWVNDLRNQIERTRILSTVYRSASA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPSIASARAKVAGAGAIL+PVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR
Sbjct: 361 LGSRLPSIASARAKVAGAGAILKPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNEI 480
           RRAM SHS AEE GSSPK SPRKMES EPLRSSP+E VEA E    PESSTT +QW+NEI
Sbjct: 421 RRAMTSHSTAEENGSSPKSSPRKMESFEPLRSSPQEIVEATEL---PESSTTTIQWTNEI 480

Query: 481 EYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVAEEIRE 540
           E SYSEEINP+G+ DEL+DD Q LM +IQDEQ+TEVELWQQLEHEL+D+ E DVA+EIRE
Sbjct: 481 ECSYSEEINPDGMRDELDDDDQALMSHIQDEQITEVELWQQLEHELHDRSEADVAKEIRE 540

Query: 541 EEAAAMAEVGQSDSSACGIKEAHRFFPAGKIMHMIDI--QSDAPDCESDSSSSRSSISEN 600
           EEAAAMAEVGQSDS   G+KEAHRFFPAGKIMH+I+I  QSDAPDCESD SSSRSS S++
Sbjct: 541 EEAAAMAEVGQSDSFTSGMKEAHRFFPAGKIMHIIEIETQSDAPDCESD-SSSRSSTSDS 600

Query: 601 SPLAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELEKEECFNLGMER 658
           SP A+ + GIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKL+KELEKEEC N GMER
Sbjct: 601 SPQAQSRTGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLVKELEKEECCNRGMER 655

BLAST of CSPI03G28360 vs. TAIR 10
Match: AT3G14075.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 795.4 bits (2053), Expect = 3.4e-230
Identity = 446/663 (67.27%), Postives = 519/663 (78.28%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL  +G  D  D +   +++   L  DRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKL-IAGPSDVDDENSEASASRPSLRIDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFL+KRQG LHV  VFG +DS++L+G+
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGS 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           E+ TELKYLLHLLTLCWHFSKK FP FLEETGF+KENVL+ EPKAGILKPAFT+LVDHNT
Sbjct: 121 EVATELKYLLHLLTLCWHFSKKSFPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           K  LLLIRGTHSIKDTLTAATGA+VPFHH+VV+E GVSNLVLGYAHCGMVAAAR IAKL+
Sbjct: 181 KYFLLLIRGTHSIKDTLTAATGAIVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLA 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLK L QY  Y IK+VGHSLGGGTAALLTYI+REQK LS  +CVTFAPAACMTWELA
Sbjct: 241 TPCLLKGLEQYPDYKIKIVGHSLGGGTAALLTYIMREQKMLSTATCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           +SGN+FI SVINGADLVPTFSAA+VDDLR EVTASAW+NDLRNQIE TRILSTVYRSA+A
Sbjct: 301 DSGNDFIVSVINGADLVPTFSAAAVDDLRAEVTASAWLNDLRNQIEHTRILSTVYRSATA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPS+A+A+AKVAGAGA+LRPVSSGTQVVM+RAQSM     TRP+L +SSWSC+GPR
Sbjct: 361 LGSRLPSMATAKAKVAGAGAMLRPVSSGTQVVMRRAQSML----TRPALSISSWSCMGPR 420

Query: 421 RRAMASHSVAE-EGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNE 480
           RRA A+ S++E +  +S   S    E+ +PL  + EE                  +W +E
Sbjct: 421 RRASATQSISEHQLDTSEAMSQDIPETSDPLLVTDEEITG---------------KWKSE 480

Query: 481 IEYSYSEEINPE-GIT--DELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKG-----E 540
            E S  EE +P  G T  DE ED  +    + ++E+MTE ELWQQLEH+LY        E
Sbjct: 481 AECSNYEETSPRLGATDLDECEDPAEM---DTREERMTEAELWQQLEHDLYHDSSEQPEE 540

Query: 541 PDVAEEIREEEAAAMAEVGQS--DSSACGIKEAHRFFPAGKIMHMIDIQSDA--PDCESD 600
            DVA+EI+EEE A +AE G +  +S    +KE+ RF PAGKIMH++ ++ +A  P+ E D
Sbjct: 541 TDVAKEIKEEEEAVIAEAGVAPPESQTAEMKESRRFLPAGKIMHIVTVRPEAVEPNEEED 600

Query: 601 SSSSRSSISEN-SPLAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELE 650
              S     E    + E ++GIFLT RSLYSK+RLSQ MISDH+MP YRRQIE+LI+EL 
Sbjct: 601 EDGSALERPETVETVEEGRVGIFLTPRSLYSKVRLSQRMISDHFMPVYRRQIERLIQELT 640

BLAST of CSPI03G28360 vs. TAIR 10
Match: AT3G14075.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 795.4 bits (2053), Expect = 3.4e-230
Identity = 446/663 (67.27%), Postives = 519/663 (78.28%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MATATMATAAGAAALLYYTLNRKL  +G  D  D +   +++   L  DRVSHRLIQAPA
Sbjct: 1   MATATMATAAGAAALLYYTLNRKL-IAGPSDVDDENSEASASRPSLRIDRVSHRLIQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFL+KRQG LHV  VFG +DS++L+G+
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLIKRQGLLHVDRVFGGKDSVELRGS 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           E+ TELKYLLHLLTLCWHFSKK FP FLEETGF+KENVL+ EPKAGILKPAFT+LVDHNT
Sbjct: 121 EVATELKYLLHLLTLCWHFSKKSFPFFLEETGFTKENVLIHEPKAGILKPAFTVLVDHNT 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           K  LLLIRGTHSIKDTLTAATGA+VPFHH+VV+E GVSNLVLGYAHCGMVAAAR IAKL+
Sbjct: 181 KYFLLLIRGTHSIKDTLTAATGAIVPFHHTVVNERGVSNLVLGYAHCGMVAAARCIAKLA 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
           TPCLLK L QY  Y IK+VGHSLGGGTAALLTYI+REQK LS  +CVTFAPAACMTWELA
Sbjct: 241 TPCLLKGLEQYPDYKIKIVGHSLGGGTAALLTYIMREQKMLSTATCVTFAPAACMTWELA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           +SGN+FI SVINGADLVPTFSAA+VDDLR EVTASAW+NDLRNQIE TRILSTVYRSA+A
Sbjct: 301 DSGNDFIVSVINGADLVPTFSAAAVDDLRAEVTASAWLNDLRNQIEHTRILSTVYRSATA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           LGSRLPS+A+A+AKVAGAGA+LRPVSSGTQVVM+RAQSM     TRP+L +SSWSC+GPR
Sbjct: 361 LGSRLPSMATAKAKVAGAGAMLRPVSSGTQVVMRRAQSML----TRPALSISSWSCMGPR 420

Query: 421 RRAMASHSVAE-EGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIEPPESSTTAMQWSNE 480
           RRA A+ S++E +  +S   S    E+ +PL  + EE                  +W +E
Sbjct: 421 RRASATQSISEHQLDTSEAMSQDIPETSDPLLVTDEEITG---------------KWKSE 480

Query: 481 IEYSYSEEINPE-GIT--DELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKG-----E 540
            E S  EE +P  G T  DE ED  +    + ++E+MTE ELWQQLEH+LY        E
Sbjct: 481 AECSNYEETSPRLGATDLDECEDPAEM---DTREERMTEAELWQQLEHDLYHDSSEQPEE 540

Query: 541 PDVAEEIREEEAAAMAEVGQS--DSSACGIKEAHRFFPAGKIMHMIDIQSDA--PDCESD 600
            DVA+EI+EEE A +AE G +  +S    +KE+ RF PAGKIMH++ ++ +A  P+ E D
Sbjct: 541 TDVAKEIKEEEEAVIAEAGVAPPESQTAEMKESRRFLPAGKIMHIVTVRPEAVEPNEEED 600

Query: 601 SSSSRSSISEN-SPLAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRRQIEKLIKELE 650
              S     E    + E ++GIFLT RSLYSK+RLSQ MISDH+MP YRRQIE+LI+EL 
Sbjct: 601 EDGSALERPETVETVEEGRVGIFLTPRSLYSKVRLSQRMISDHFMPVYRRQIERLIQELT 640

BLAST of CSPI03G28360 vs. TAIR 10
Match: AT4G16070.1 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 630.9 bits (1626), Expect = 1.1e-180
Identity = 367/670 (54.78%), Postives = 460/670 (68.66%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MA   M TA GA  +LY    R + +   +DD    G +       G  R+  R  QAPA
Sbjct: 1   MAAGVMVTATGAVVILYLLSRRIVWARNGEDD---SGGELGKSGRSGRRRIVRRPAQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPI DLAFGIN+L++RQGN    SV+   + I+LKG 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIADLAFGINYLMRRQGNFPTASVYAGSNCIELKGP 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           E+I +L  LL  LTLC  FSKKPF +FLE  G++ E+VLLQ+PKAGI++PAFTI+ D N+
Sbjct: 121 EIIMDLTELLRFLTLCMLFSKKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNS 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSV+H+GG+SNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVLHDGGLSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
            PCLLKAL +   + +++VGHSLGGGTA+LLTYILREQKE +  +C TFAPAACMTW+LA
Sbjct: 241 VPCLLKALDENPSFKVQIVGHSLGGGTASLLTYILREQKEFASATCFTFAPAACMTWDLA 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESG  FIT++ING+DLVPTFSA+SVDDLR EVT+S+W NDLR+Q+E TR+LS VYRSA+A
Sbjct: 301 ESGKHFITTIINGSDLVPTFSASSVDDLRSEVTSSSWSNDLRDQVEHTRVLSVVYRSATA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           +GSRLPSIASA+AKVAGAGAILRPVSSGTQV++KRAQ +AQA     S  LSSWSCIGPR
Sbjct: 361 IGSRLPSIASAKAKVAGAGAILRPVSSGTQVMLKRAQDVAQAVVQTRST-LSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIE-----PPESSTTAMQ 480
           RRA++S  +  +    P+ S    E     R S E  +    AI+       E S+++  
Sbjct: 421 RRAISS-QLNSKVTDMPEASAIMAE-----RRSTEALLAETVAIDRKGHKRTEHSSSSSS 480

Query: 481 WSNEIEYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVA 540
            S+  E    EE  P    D++  +  ++     +E +TE ELW +L+ EL  + E +  
Sbjct: 481 ESDRDEPDEEEEEEPLISIDQVIAETSSI-----EEDVTEGELWDELDREL-TRQENERD 540

Query: 541 EEIREEEAAAMAEV---------------GQSDS----SACGIKEAHRFFPAGKIMHMID 600
            E  EEEAAA  E+               GQ+ S    S+  + E  RF+P GKIMH++ 
Sbjct: 541 SEAMEEEAAAAKEITEEETVITGGGDSSTGQNQSPVSASSMDLIENQRFYPPGKIMHIVS 600

Query: 601 IQSDAPDCESDSSSSRSSISENSPLAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRR 647
           +       E++S + R  +         ++ I+ T R LY K+RLS+TMI+DHYMP Y++
Sbjct: 601 V------TETESETERDEVVVVGTTTVERVRIYETPRELYRKIRLSRTMINDHYMPMYKK 648

BLAST of CSPI03G28360 vs. TAIR 10
Match: AT4G16070.2 (Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 )

HSP 1 Score: 606.7 bits (1563), Expect = 2.2e-173
Identity = 360/670 (53.73%), Postives = 452/670 (67.46%), Query Frame = 0

Query: 1   MATATMATAAGAAALLYYTLNRKLHSSGDQDDGDVDGNDASTHALLGGDRVSHRLIQAPA 60
           MA   M TA GA  +LY    R + +   +DD    G +       G  R+  R  QAPA
Sbjct: 1   MAAGVMVTATGAVVILYLLSRRIVWARNGEDD---SGGELGKSGRSGRRRIVRRPAQAPA 60

Query: 61  TWLETISTLSETLRFTYSETLGKWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGT 120
           TWLETISTLSETLRFTYSETLGKWPI DLAFGIN+L++RQGN    SV+   + I+LKG 
Sbjct: 61  TWLETISTLSETLRFTYSETLGKWPIADLAFGINYLMRRQGNFPTASVYAGSNCIELKGP 120

Query: 121 EMITELKYLLHLLTLCWHFSKKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNT 180
           E+I +L  LL  LTLC  FSKKPF +FLE  G++ E+VLLQ+PKAGI++PAFTI+ D N+
Sbjct: 121 EIIMDLTELLRFLTLCMLFSKKPFAVFLESAGYTHEDVLLQKPKAGIMQPAFTIIRDTNS 180

Query: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVVHEGGVSNLVLGYAHCGMVAAARWIAKLS 240
           KCILLLIRGTHSIKDTLTAATGAVVPFHHSV+H+GG+SNLVLGYAHCGMVAAARWIAKLS
Sbjct: 181 KCILLLIRGTHSIKDTLTAATGAVVPFHHSVLHDGGLSNLVLGYAHCGMVAAARWIAKLS 240

Query: 241 TPCLLKALGQYSGYNIKVVGHSLGGGTAALLTYILREQKELSITSCVTFAPAACMTWELA 300
            PCLLKAL +   + +++VGHSLGGGTA+LLTYILREQKE +  +C TFAP        A
Sbjct: 241 VPCLLKALDENPSFKVQIVGHSLGGGTASLLTYILREQKEFASATCFTFAP--------A 300

Query: 301 ESGNEFITSVINGADLVPTFSAASVDDLRGEVTASAWVNDLRNQIERTRILSTVYRSASA 360
           ESG  FIT++ING+DLVPTFSA+SVDDLR EVT+S+W NDLR+Q+E TR+LS VYRSA+A
Sbjct: 301 ESGKHFITTIINGSDLVPTFSASSVDDLRSEVTSSSWSNDLRDQVEHTRVLSVVYRSATA 360

Query: 361 LGSRLPSIASARAKVAGAGAILRPVSSGTQVVMKRAQSMAQAAWTRPSLHLSSWSCIGPR 420
           +GSRLPSIASA+AKVAGAGAILRPVSSGTQV++KRAQ +AQA     S  LSSWSCIGPR
Sbjct: 361 IGSRLPSIASAKAKVAGAGAILRPVSSGTQVMLKRAQDVAQAVVQTRST-LSSWSCIGPR 420

Query: 421 RRAMASHSVAEEGGSSPKPSPRKMESCEPLRSSPEETVEAIEAIE-----PPESSTTAMQ 480
           RRA++S  +  +    P+ S    E     R S E  +    AI+       E S+++  
Sbjct: 421 RRAISS-QLNSKVTDMPEASAIMAE-----RRSTEALLAETVAIDRKGHKRTEHSSSSSS 480

Query: 481 WSNEIEYSYSEEINPEGITDELEDDGQTLMGNIQDEQMTEVELWQQLEHELYDKGEPDVA 540
            S+  E    EE  P    D++  +  ++     +E +TE ELW +L+ EL  + E +  
Sbjct: 481 ESDRDEPDEEEEEEPLISIDQVIAETSSI-----EEDVTEGELWDELDREL-TRQENERD 540

Query: 541 EEIREEEAAAMAEV---------------GQSDS----SACGIKEAHRFFPAGKIMHMID 600
            E  EEEAAA  E+               GQ+ S    S+  + E  RF+P GKIMH++ 
Sbjct: 541 SEAMEEEAAAAKEITEEETVITGGGDSSTGQNQSPVSASSMDLIENQRFYPPGKIMHIVS 600

Query: 601 IQSDAPDCESDSSSSRSSISENSPLAECKIGIFLTSRSLYSKLRLSQTMISDHYMPAYRR 647
           +       E++S + R  +         ++ I+ T R LY K+RLS+TMI+DHYMP Y++
Sbjct: 601 V------TETESETERDEVVVVGTTTVERVRIYETPRELYRKIRLSRTMINDHYMPMYKK 640

BLAST of CSPI03G28360 vs. TAIR 10
Match: AT2G42450.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 2.6e-28
Identity = 93/331 (28.10%), Postives = 153/331 (46.22%), Query Frame = 0

Query: 26  SSGDQDDGDVDGN-DAS--THALLGGDRVSHRLIQAPATWLETISTLSETLRFTYSETLG 85
           SS D+D GD     D +  T AL    ++    +      L    +LSE +       LG
Sbjct: 86  SSSDEDTGDGKWKLDLAWLTKALEPAMQLRRWALPTGEKSLPGSRSLSEIIASIQRSRLG 145

Query: 86  --KWPIGDLAFGINFLLKRQGNLHVGSVFGNEDSIQLKGTEMITELKYLLHLLTLCWHFS 145
              W  GDL  G+  +  RQ +L   S F +   +++     + +L Y   L   C+  S
Sbjct: 146 IEGWTFGDLTIGLYLIYLRQASL---SPFEDVKGVEVVSESTVYDLIYNAELAKGCYRDS 205

Query: 146 KKPFPLFLEETGFSKENVLLQEPKAGILKPAFTILVDHNTKCILLLIRGTHSIKDTLTAA 205
                   + T   + N+L     + +++P + I VDH  K ++  IRGTH+I D +T  
Sbjct: 206 VSG---LAKNTMLRENNILKFVKDSSVMRPGYYIGVDHRRKLVVFGIRGTHTIYDLIT-- 265

Query: 206 TGAVVPFHHSVVHEGGVSNLVLGYA-HCGMVAAARWIAKLSTPCLLKALGQYSGYNIKVV 265
                     +V          GY+ H G   AARW        + + L +Y GY +++V
Sbjct: 266 ---------DIVSSSDEEVTFEGYSTHFGTAEAARWFLNHELQTIRRCLAKYEGYKLRLV 325

Query: 266 GHSLGGGTAALLTYILREQK------ELSITSCVTFAPAACMTWELAESGNEFITSVING 325
           GHSLGG  A+L+  +L++        +  I S V +A   C++ ELAE+ +EF+T+++  
Sbjct: 326 GHSLGGAIASLMAIMLKKMPREELGFDAEIISAVGYATPPCVSKELAENCSEFVTTIVMQ 385

Query: 326 ADLVPTFSAASVDDLRGEVTASAWVNDLRNQ 345
            D++P  SAAS+  LR E+  + W + +  +
Sbjct: 386 DDIIPRLSAASLARLRNEILQTDWTSVIEKE 399

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9Y4D21.0e-1333.15Diacylglycerol lipase-alpha OS=Homo sapiens OX=9606 GN=DAGLA PE=1 SV=3[more]
Q6WQJ13.0e-1333.52Diacylglycerol lipase-alpha OS=Mus musculus OX=10090 GN=Dagla PE=1 SV=2[more]
Q5YLM13.0e-1333.52Diacylglycerol lipase-alpha OS=Rattus norvegicus OX=10116 GN=Dagla PE=1 SV=1[more]
P0C1S93.3e-1227.23Diacylglycerol lipase-beta OS=Rattus norvegicus OX=10116 GN=Daglb PE=1 SV=1[more]
Q91WC91.6e-1127.75Diacylglycerol lipase-beta OS=Mus musculus OX=10090 GN=Daglb PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LCM60.0e+0099.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G644860 PE=4 SV=1[more]
A0A1S3CC710.0e+0096.80uncharacterized protein LOC103499170 OS=Cucumis melo OX=3656 GN=LOC103499170 PE=... [more]
A0A5A7UE470.0e+0097.18Mono-/di-acylglycerol lipase isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1GYN50.0e+0089.98uncharacterized protein LOC111458044 OS=Cucurbita moschata OX=3662 GN=LOC1114580... [more]
A0A6J1IRP80.0e+0089.80uncharacterized protein LOC111479893 OS=Cucurbita maxima OX=3661 GN=LOC111479893... [more]
Match NameE-valueIdentityDescription
XP_004144431.10.0e+0099.70uncharacterized protein LOC101203983 [Cucumis sativus] >KGN58447.1 hypothetical ... [more]
XP_008460311.10.0e+0096.80PREDICTED: uncharacterized protein LOC103499170 [Cucumis melo][more]
KAA0053494.10.0e+0097.18Mono-/di-acylglycerol lipase isoform 1 [Cucumis melo var. makuwa] >TYK19096.1 Mo... [more]
XP_038901142.10.0e+0094.06uncharacterized protein LOC120088127 isoform X1 [Benincasa hispida] >XP_03890114... [more]
XP_022956309.10.0e+0089.98uncharacterized protein LOC111458044 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G14075.13.4e-23067.27Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT3G14075.23.4e-23067.27Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT4G16070.11.1e-18054.78Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT4G16070.22.2e-17353.73Mono-/di-acylglycerol lipase, N-terminal;Lipase, class 3 [more]
AT2G42450.12.6e-2828.10alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005592Mono-/di-acylglycerol lipase, N-terminalPFAMPF03893Lipase3_Ncoord: 50..130
e-value: 2.1E-19
score: 69.3
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 185..322
e-value: 2.8E-23
score: 82.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 149..354
e-value: 1.9E-30
score: 107.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 138..322
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 425..455
NoneNo IPR availablePANTHERPTHR46023:SF6LIPASE CLASS 3 FAMILY PROTEINcoord: 1..649
NoneNo IPR availablePANTHERPTHR46023LIPASE CLASS 3 PROTEIN-LIKEcoord: 1..649
NoneNo IPR availableCDDcd00519Lipase_3coord: 174..321
e-value: 3.88617E-37
score: 136.452

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G28360.1CSPI03G28360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006629 lipid metabolic process