CSPI03G03560 (gene) Cucumber (PI 183967) v1

BLAST of CSPI03G03560 vs. ExPASy Swiss-Prot
Match: Q3AD36 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=gatA PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 1.0e-44
Identity = 146/498 (29.32%), Postives = 231/498 (46.39%), Query Frame = 0

Query: 148 DIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADEL 207
           ++ ++S  E+ E IK ++IS+ E+ +    R++     ++A V+ T EL  K A+E DE 
Sbjct: 2   ELYYLSAKEITEKIKAKEISAVEVAKATFDRIEAVEPKIQAYVTVTRELGLKMAREVDEK 61

Query: 208 FAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAK 267
            A+G   GPL G+P  +KD +S  G +TT  SK  +  +   +A V ++LK AGAV   K
Sbjct: 62  IARGEDPGPLAGVPVAIKDNMSTAGIRTTCSSKILENYIPPYDATVVEKLKEAGAVFTGK 121

Query: 268 LVTGSLAYDDIWFGGR---TRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTY 327
                 A        R   TRNPW++E    GSS G AA  +AG    A+GS+T GS+  
Sbjct: 122 TNLDEFAMGSSTENSRFFPTRNPWDLERVPGGSSGGSAASVAAGEAVVALGSDTGGSIRQ 181

Query: 328 PAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSV 387
           PAA CG+  ++PT+G V R G+++ + SLD++GPF R   D A++L+VI G DP D +S 
Sbjct: 182 PAAFCGIVGLKPTYGAVSRYGLVAFASSLDQIGPFARTVEDAALLLNVIAGHDPKDSTSA 241

Query: 388 ESSLDDPFSI---DISKLTVG-----YLDDADM-------EVVRILSSKGVNMVPFNLSY 447
           +    D  S    DI  L +G     ++D  D        + +++L S G      +L +
Sbjct: 242 DIEYPDYLSFLNQDIKGLKIGLPKEYFIDGIDAGVKKAIDDAIKVLESLGAVFEEVSLPH 301

Query: 448 SVDS--VQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQD-----------QWPTELRRAR 507
           +  S  V  +I        LA +D   R G  D +EA+D            +  E++R  
Sbjct: 302 TKYSLPVYYLIAPAEASSNLARYD-GVRYGYRD-FEAEDVVEMFSRTRAEGFGAEVKRRI 361

Query: 508 LIP----AVDYVQAQRARGKLIRE-VRESFN-----VDALIGNATDWEKVCMG------- 567
           ++     +  Y  A   +   +R  ++E F+     VD L+   T       G       
Sbjct: 362 MLGTYALSAGYYDAYYLKALKVRTLIKEDFDRAFTKVDLLLTPTTPTPAFKFGEKTSDPV 421

Query: 568 ------------NLVGLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALA 586
                       NL GLP + VP GF                 ++  +   P  +   L 
Sbjct: 422 SMYLSDIFTMAVNLAGLPGISVPAGFDG------------HLPVSFQLIGKPFDEGTLLK 481

BLAST of CSPI03G03560 vs. ExPASy Swiss-Prot
Match: B2V855 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Sulfurihydrogenibium sp. (strain YO3AOP1) OX=436114 GN=gatA PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.1e-41
Identity = 137/493 (27.79%), Postives = 236/493 (47.87%), Query Frame = 0

Query: 153 SVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGV 212
           S+ EL +L+K++++   E+V  F++R  +    ++A V+  ++LA ++AK+ D+   +  
Sbjct: 7   SLKELSDLVKSKEVKPSEIVEAFIERKNQVEPKIKAYVTALDDLALEKAKKRDQELTKLE 66

Query: 213 YLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAK----- 272
            +  L G+P  +KD IS    KTT  SK  +  V   +A V ++LKS G V+  K     
Sbjct: 67  NIPDLFGLPIAIKDNISTKDIKTTCSSKMLENFVPVYDATVIERLKSQGYVITGKTNLDE 126

Query: 273 LVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAA 332
              GS   +  +F   TRNPW++E    GSS G AA  ++GM P ++GS+T GS+  PAA
Sbjct: 127 FAMGSSTENSAFF--PTRNPWDLERVPGGSSGGSAAVVASGMAPASLGSDTGGSIRQPAA 186

Query: 333 RCGVTAIRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESS 392
            CGV  ++PT+G V R G+++ + SLD++GPF R   D A++++VI GKDP D +S    
Sbjct: 187 FCGVVGLKPTYGRVSRYGLVAFASSLDQIGPFGRTVEDVAMIMNVISGKDPKDSTSRSIP 246

Query: 393 LD---DPFSIDISKLTVG-----YLDDADMEV-------VRILSSKGVNMVPFNLSYSVD 452
           +    +  + D+  L +G     Y +D + ++       V+ L  +G+     +L Y+  
Sbjct: 247 VPNYLESLNKDVKGLKIGLPKEFYTEDLNPQIKEIILNAVKQLEKEGMTAHEISLPYTKY 306

Query: 453 SVQG--IINFTMDVDMLAHFD---------EWQRLGLDDEYEAQDQWPTELRRARLIPA- 512
           +++   II  +     LA FD         E++ L         + +  E++R  +I   
Sbjct: 307 AIETYYIIAPSEASSNLARFDGVRYGYRAKEYKNLEEMYSKTRDEGFGAEVKRRIMIGTY 366

Query: 513 --------VDYVQAQRARGKLIREVRESF-NVDALIGNATDWEKVCMG------------ 572
                     Y++AQ+ R  + ++   +F  VD +I   T      +G            
Sbjct: 367 ALSSGYYDAYYLKAQKVRTLIYQDYMNAFEKVDVIITPTTPDVAFKIGEKSNDPIQMYLS 426

Query: 573 -------NLVGLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAY 586
                  N+  +P + +P GFK+  N P          +   I   P  +   L +A  +
Sbjct: 427 DIFTVSANMATVPALSIPCGFKD--NLP----------VGMQIIGKPFDEETILQVAYKF 485

BLAST of CSPI03G03560 vs. ExPASy Swiss-Prot
Match: P63497 (Putative amidase AmiD OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=amiD PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.1e-41
Identity = 138/467 (29.55%), Postives = 219/467 (46.90%), Query Frame = 0

Query: 145 NEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEA 204
           +ED I+ + + E+ +LI+T Q++S E+    L+R++R +  L++      E A   A+ A
Sbjct: 13  DEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAA 72

Query: 205 DELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVL 264
           D   A+G Y G LHG+P G+KD+       T  G+  F++     +A V  +L++AGAV+
Sbjct: 73  DADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVI 132

Query: 265 VAKLVTGSLAYDDIWFGGRTR-NPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMT 324
           + KL     AY        T  NPW+   ++  SS+G    T+AG+   +IGS+T GS+ 
Sbjct: 133 IGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSIR 192

Query: 325 YPAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSS 384
           +P + CGVT I+PT+G V R GV+ ++ S D +GP  R+A D AV+L VI G D HD S 
Sbjct: 193 FPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGSDIHDPSC 252

Query: 385 VESSLDD-----------PFSIDISKLTVGYLDDADM--EVVRILSSKGVNMVPFNLSYS 444
               + D              +D S+ T    D   M  +VV+ L   G  ++   L   
Sbjct: 253 SAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPAL 312

Query: 445 VDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLIPAVDYVQAQRAR 504
              V            +AH D +      DEY    +    +     + AV+Y      R
Sbjct: 313 APMVAAFGKMRAVETAIAHADTYPARA--DEYGPIMR--AMIDAGHRLAAVEYQTLTERR 372

Query: 505 GKLIREVRESF-NVDALIGNATDWEKVCMGNLVGL-------PIVVVPTGFKNISNPPS- 564
            +  R +R  F +VD L+  +       +  + GL         + +PT   N+S  P+ 
Sbjct: 373 LEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPFNVSGNPAI 432

Query: 565 ---TGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPI 586
               GTT R T +          +H+ +    A+Q  T +HR +PP+
Sbjct: 433 CLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV 475

BLAST of CSPI03G03560 vs. ExPASy Swiss-Prot
Match: P9WQ92 (Putative amidase AmiD OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=amiD PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.1e-41
Identity = 138/467 (29.55%), Postives = 219/467 (46.90%), Query Frame = 0

Query: 145 NEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEA 204
           +ED I+ + + E+ +LI+T Q++S E+    L+R++R +  L++      E A   A+ A
Sbjct: 13  DEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAA 72

Query: 205 DELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVL 264
           D   A+G Y G LHG+P G+KD+       T  G+  F++     +A V  +L++AGAV+
Sbjct: 73  DADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVI 132

Query: 265 VAKLVTGSLAYDDIWFGGRTR-NPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMT 324
           + KL     AY        T  NPW+   ++  SS+G    T+AG+   +IGS+T GS+ 
Sbjct: 133 IGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSIR 192

Query: 325 YPAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSS 384
           +P + CGVT I+PT+G V R GV+ ++ S D +GP  R+A D AV+L VI G D HD S 
Sbjct: 193 FPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGSDIHDPSC 252

Query: 385 VESSLDD-----------PFSIDISKLTVGYLDDADM--EVVRILSSKGVNMVPFNLSYS 444
               + D              +D S+ T    D   M  +VV+ L   G  ++   L   
Sbjct: 253 SAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPAL 312

Query: 445 VDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLIPAVDYVQAQRAR 504
              V            +AH D +      DEY    +    +     + AV+Y      R
Sbjct: 313 APMVAAFGKMRAVETAIAHADTYPARA--DEYGPIMR--AMIDAGHRLAAVEYQTLTERR 372

Query: 505 GKLIREVRESF-NVDALIGNATDWEKVCMGNLVGL-------PIVVVPTGFKNISNPPS- 564
            +  R +R  F +VD L+  +       +  + GL         + +PT   N+S  P+ 
Sbjct: 373 LEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPFNVSGNPAI 432

Query: 565 ---TGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPI 586
               GTT R T +          +H+ +    A+Q  T +HR +PP+
Sbjct: 433 CLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV 475

BLAST of CSPI03G03560 vs. ExPASy Swiss-Prot
Match: P9WQ93 (Putative amidase AmiD OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=amiD PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 4.1e-41
Identity = 138/467 (29.55%), Postives = 219/467 (46.90%), Query Frame = 0

Query: 145 NEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEA 204
           +ED I+ + + E+ +LI+T Q++S E+    L+R++R +  L++      E A   A+ A
Sbjct: 13  DEDAISKLELTEVADLIRTRQLTSAEVTESTLRRIERLDPQLKSYAFVMPETALAAARAA 72

Query: 205 DELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVL 264
           D   A+G Y G LHG+P G+KD+       T  G+  F++     +A V  +L++AGAV+
Sbjct: 73  DADIARGHYEGVLHGVPIGVKDLCYTVDAPTAAGTTIFRDFRPAYDATVVARLRAAGAVI 132

Query: 265 VAKLVTGSLAYDDIWFGGRTR-NPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMT 324
           + KL     AY        T  NPW+   ++  SS+G    T+AG+   +IGS+T GS+ 
Sbjct: 133 IGKLAMTEGAYLGYHPSLPTPVNPWDPTAWAGVSSSGCGVATAAGLCFGSIGSDTGGSIR 192

Query: 325 YPAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSS 384
           +P + CGVT I+PT+G V R GV+ ++ S D +GP  R+A D AV+L VI G D HD S 
Sbjct: 193 FPTSMCGVTGIKPTWGRVSRHGVVELAASYDHVGPITRSAHDAAVLLSVIAGSDIHDPSC 252

Query: 385 VESSLDD-----------PFSIDISKLTVGYLDDADM--EVVRILSSKGVNMVPFNLSYS 444
               + D              +D S+ T    D   M  +VV+ L   G  ++   L   
Sbjct: 253 SAEPVPDYAADLALTRIPRVGVDWSQTTSFDEDTTAMLADVVKTLDDIGWPVIDVKLPAL 312

Query: 445 VDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLIPAVDYVQAQRAR 504
              V            +AH D +      DEY    +    +     + AV+Y      R
Sbjct: 313 APMVAAFGKMRAVETAIAHADTYPARA--DEYGPIMR--AMIDAGHRLAAVEYQTLTERR 372

Query: 505 GKLIREVRESF-NVDALIGNATDWEKVCMGNLVGL-------PIVVVPTGFKNISNPPS- 564
            +  R +R  F +VD L+  +       +  + GL         + +PT   N+S  P+ 
Sbjct: 373 LEFTRSLRRVFHDVDILLMPSAGIASPTLETMRGLGQDPELTARLAMPTAPFNVSGNPAI 432

Query: 565 ---TGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPI 586
               GTT R T +          +H+ +    A+Q  T +HR +PP+
Sbjct: 433 CLPAGTTARGTPLGVQFIGREFDEHLLVRAGHAFQQVTGYHRRRPPV 475

BLAST of CSPI03G03560 vs. ExPASy TrEMBL
Match: A0A0A0L5U7 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G039330 PE=4 SV=1)

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 606/610 (99.34%), Postives = 607/610 (99.51%), Query Frame = 0

Query: 1   MASTPTINCFVV-FFFFFFTVSVRSSISSHFFSFPQCSWRRFTLMDSKKDSECLENMGRT 60
           MASTPTINCFVV FFFFFFTVSVRSSISSHF SFPQCSWRRFTLMDSKKDSECLENMGRT
Sbjct: 75  MASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMDSKKDSECLENMGRT 134

Query: 61  SVLKCVFRALDAGFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPE 120
           SVLKCVFRALDA FFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPE
Sbjct: 135 SVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPE 194

Query: 121 WANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRL 180
           WANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRL
Sbjct: 195 WANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRL 254

Query: 181 KRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGS 240
           KRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGS
Sbjct: 255 KRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGS 314

Query: 241 KSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSA 300
           KSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSA
Sbjct: 315 KSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSA 374

Query: 301 GPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPF 360
           GPAACTSAGMVPFAIGSET+GSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPF
Sbjct: 375 GPAACTSAGMVPFAIGSETIGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPF 434

Query: 361 CRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDADMEVVRILSSKG 420
           CRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDADMEVVRILSSKG
Sbjct: 435 CRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDADMEVVRILSSKG 494

Query: 421 VNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLIP 480
           VNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLIP
Sbjct: 495 VNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLIP 554

Query: 481 AVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPIVVVPTGFKNISNP 540
           AVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPIVVVPTGFKNISNP
Sbjct: 555 AVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPIVVVPTGFKNISNP 614

Query: 541 PSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPIDDLGPSDVLPDPPL 600
           PSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPIDDLGPSDVLPDPPL
Sbjct: 615 PSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPIDDLGPSDVLPDPPL 674

Query: 601 VSIPPRLLHL 610
           VSIPPRLLHL
Sbjct: 675 VSIPPRLLHL 684

BLAST of CSPI03G03560 vs. ExPASy TrEMBL
Match: A0A1S4DX80 (glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo OX=3656 GN=LOC103490981 PE=4 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 546/567 (96.30%), Postives = 559/567 (98.59%), Query Frame = 0

Query: 43  LMDSKKDSECLENMGRTSVLKCVFRALDAGFFNNTKRDEVLKGAEELNVPIIRANRKLVA 102
           L DSKKDSECLENMGRTS+LKCVFRA DA FFNNTKRDEVLKGAEE+NVP IRANRKLVA
Sbjct: 25  LQDSKKDSECLENMGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVA 84

Query: 103 SENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIK 162
           SENGGLHNPS LVFNPEWANEN RHKSKRFCYPPV G+KRPNNEDDIAFMSVLELGELIK
Sbjct: 85  SENGGLHNPSPLVFNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIK 144

Query: 163 TEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY 222
           TEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY
Sbjct: 145 TEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY 204

Query: 223 GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG 282
           GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG
Sbjct: 205 GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG 264

Query: 283 RTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVG 342
           RTRNPWNIEEF+TGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVG
Sbjct: 265 RTRNPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVG 324

Query: 343 RSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVG 402
           RSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVG
Sbjct: 325 RSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVG 384

Query: 403 YLDDADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEY 462
           Y+DDADMEVV+ILSSKGVNM+PFNLSYSVDSVQGI+NFTMDVDMLAHFDEWQRLGLDDEY
Sbjct: 385 YVDDADMEVVQILSSKGVNMIPFNLSYSVDSVQGIVNFTMDVDMLAHFDEWQRLGLDDEY 444

Query: 463 EAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLV 522
           EAQDQWPTELRRARLIPAVDYVQAQRARGKLIRE+RESFNVDALIGNATDWEKVCMGNLV
Sbjct: 445 EAQDQWPTELRRARLIPAVDYVQAQRARGKLIREIRESFNVDALIGNATDWEKVCMGNLV 504

Query: 523 GLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAK 582
           GLPI+VVPTGFKNISNPPSTGTTRRK TIT+GIYAPPHRDHIALALAMAYQSATDHHRA+
Sbjct: 505 GLPIIVVPTGFKNISNPPSTGTTRRKVTITSGIYAPPHRDHIALALAMAYQSATDHHRAR 564

Query: 583 PPIDDLGPSDVLPDPPLVSIPPRLLHL 610
           PPIDDLGP+DVLPDPPLVSIPPRLLHL
Sbjct: 565 PPIDDLGPNDVLPDPPLVSIPPRLLHL 591

BLAST of CSPI03G03560 vs. ExPASy TrEMBL
Match: A0A5D3D785 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00780 PE=4 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 536/564 (95.04%), Postives = 549/564 (97.34%), Query Frame = 0

Query: 46  SKKDSECLENMGRTSVLKCVFRALDAGFFNNTKRDEVLKGAEELNVPIIRANRKLVASEN 105
           SKKDSECLENMGRTS+LKCVFRA DA FFNNTKRDEVLKGAEE+NVP IRANRKLVASEN
Sbjct: 15  SKKDSECLENMGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVASEN 74

Query: 106 GGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQ 165
           GGLHNPS LVFNPEWANEN RHKSKRFCYPPV G+KRPNNEDDIAFMSVLELGELIKTEQ
Sbjct: 75  GGLHNPSPLVFNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIKTEQ 134

Query: 166 ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK 225
           ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK
Sbjct: 135 ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK 194

Query: 226 DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR 285
           DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR
Sbjct: 195 DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR 254

Query: 286 NPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRSG 345
           NPWNIEEF+TGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRSG
Sbjct: 255 NPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRSG 314

Query: 346 VMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLD 405
                   DKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGY+D
Sbjct: 315 --------DKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYVD 374

Query: 406 DADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQ 465
           DADMEVV+ILSSKGVNM+PFNLSYSVDSVQGI+NFTMDVDMLAHFDEWQRLGLDDEYEAQ
Sbjct: 375 DADMEVVQILSSKGVNMIPFNLSYSVDSVQGIVNFTMDVDMLAHFDEWQRLGLDDEYEAQ 434

Query: 466 DQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLP 525
           DQWPTELRRARLIPAVDYVQAQRARGKLIRE+RESFNVDALIGNATDWEKVCMGNLVGLP
Sbjct: 435 DQWPTELRRARLIPAVDYVQAQRARGKLIREIRESFNVDALIGNATDWEKVCMGNLVGLP 494

Query: 526 IVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPI 585
           I+VVPTGFKNISNPPSTGTTRRK TIT+GIYAPPHRDHIALALAMAYQSATDHHRA+PPI
Sbjct: 495 IIVVPTGFKNISNPPSTGTTRRKVTITSGIYAPPHRDHIALALAMAYQSATDHHRARPPI 554

Query: 586 DDLGPSDVLPDPPLVSIPPRLLHL 610
           DDLGP+DVLPDPPLVSIPPRLLHL
Sbjct: 555 DDLGPNDVLPDPPLVSIPPRLLHL 570

BLAST of CSPI03G03560 vs. ExPASy TrEMBL
Match: A0A6J1KX47 (uncharacterized protein LOC111498360 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498360 PE=4 SV=1)

HSP 1 Score: 1021.9 bits (2641), Expect = 1.1e-294
Identity = 509/604 (84.27%), Postives = 553/604 (91.56%), Query Frame = 0

Query: 8   NC--FVVFFFFFFTVSVRSSISSHFFSFPQCSWRRFTLMDSKKDSECLENMGRTSVLKCV 67
           NC  FV F F FF V +RSSIS +F S  Q S   FT MD  KD+E  +N G+ S     
Sbjct: 19  NCFFFVFFVFIFFAVPLRSSISPNFSSSDQ-SRPCFTAMDRNKDTESFKNGGKRS----F 78

Query: 68  FRALDAGFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPEWANENV 127
           FRA DA FF+ T+ DEVLKGAEE N P+IRANRKLVASENGGLHNPS LVF+  WANE  
Sbjct: 79  FRAFDAEFFSKTEMDEVLKGAEEFNFPVIRANRKLVASENGGLHNPSPLVFSSNWANEGF 138

Query: 128 RHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRLKRYNHV 187
           +HKS+RFCYPPV G+KRP NE+DIAFMSVLELGELIKT+QI+SQELV IFLQRLKRY++V
Sbjct: 139 QHKSRRFCYPPVFGVKRPKNEEDIAFMSVLELGELIKTKQITSQELVGIFLQRLKRYDYV 198

Query: 188 LEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQ 247
           L+AVVSFTE+LAYKQAKEADELFA+GVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQ
Sbjct: 199 LQAVVSFTEDLAYKQAKEADELFARGVYLGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQ 258

Query: 248 VIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSAGPAACT 307
           VIDVEAWVY+QLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEF+TGSSAGPAACT
Sbjct: 259 VIDVEAWVYQQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFTTGSSAGPAACT 318

Query: 308 SAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPFCRNAVD 367
           SAG+VPFAIGSET GSMTYPA+RCGVTA+RPTFGTVGRSGVMSISESLDKLGPFCRNA+D
Sbjct: 319 SAGLVPFAIGSETAGSMTYPASRCGVTALRPTFGTVGRSGVMSISESLDKLGPFCRNAID 378

Query: 368 CAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDADMEVVRILSSKGVNMVPF 427
           CAV+LDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDADMEVVR+LSSKG NMVPF
Sbjct: 379 CAVILDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDADMEVVRVLSSKGANMVPF 438

Query: 428 NLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLIPAVDYVQ 487
           NL+YS+DSVQGI+NFTMD+DMLAHFDEWQR GLD EYEAQDQWPTELRRARLIPAVDY+Q
Sbjct: 439 NLTYSLDSVQGILNFTMDIDMLAHFDEWQRSGLDHEYEAQDQWPTELRRARLIPAVDYLQ 498

Query: 488 AQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPIVVVPTGFKNISNPPSTGTT 547
           AQRARGKLIREV+ESFNVDALIGNATDWE+VCMGNLVGLP++VVPTGFKNISNPP+TGTT
Sbjct: 499 AQRARGKLIREVKESFNVDALIGNATDWERVCMGNLVGLPVMVVPTGFKNISNPPATGTT 558

Query: 548 RRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPIDDLGPSDVLPDPPLVSIPPR 607
           RR+T ITTGIYAPPH+DHIALALAMAYQSATDHHRA+PPIDDLGP D +P+PP V IPPR
Sbjct: 559 RRRTAITTGIYAPPHKDHIALALAMAYQSATDHHRARPPIDDLGPHDAIPEPPSVPIPPR 617

Query: 608 LLHL 610
           LL +
Sbjct: 619 LLRV 617

BLAST of CSPI03G03560 vs. ExPASy TrEMBL
Match: A0A6J1G4E4 (uncharacterized protein LOC111450695 OS=Cucurbita moschata OX=3662 GN=LOC111450695 PE=4 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 2.3e-292
Identity = 511/632 (80.85%), Postives = 557/632 (88.13%), Query Frame = 0

Query: 3   STPTINC--FVVFFFFFFTVSVRSSISSHFFSFPQCSWRRFTLMDS-------------- 62
           +T   NC  FV F FFFF V +RSSIS +F S  Q S   FT M +              
Sbjct: 14  TTVASNCFFFVFFVFFFFAVPLRSSISPNFSSSDQ-SRPCFTAMGATYKKIWKLLCEKVG 73

Query: 63  ---------KKDSECLENMGRTSVLKCVFRALDAGFFNNTKRDEVLKGAEELNVPIIRAN 122
                     KD+E  +N G+ S     FRA DA FF+NT+ DEVLKGAEELN+P+IRAN
Sbjct: 74  VHYRSPSYRNKDTESFKNGGKRS----FFRAFDADFFSNTEMDEVLKGAEELNLPVIRAN 133

Query: 123 RKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLEL 182
           RKLVASENGGLHNPS LVF+  WANE   HKS+RFCYPPVSG+KRP NE+DIAFMSVLEL
Sbjct: 134 RKLVASENGGLHNPSPLVFSSNWANERFLHKSRRFCYPPVSGVKRPKNEEDIAFMSVLEL 193

Query: 183 GELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPL 242
           GELIKT+QI+SQELV IFL+RLKRY++VL+AVVSFTE+LAYKQAKEADELFA+GVYLGPL
Sbjct: 194 GELIKTKQITSQELVGIFLKRLKRYDYVLQAVVSFTEDLAYKQAKEADELFARGVYLGPL 253

Query: 243 HGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDD 302
           HGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVY+QLKSAGAVLV KLVTGSLAYDD
Sbjct: 254 HGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYQQLKSAGAVLVTKLVTGSLAYDD 313

Query: 303 IWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPT 362
           IWFGGRTRNPWNIEEF+TGSSAGPAACTSAG+VPFAIGSET GSMTYPA+RCGVTA+RPT
Sbjct: 314 IWFGGRTRNPWNIEEFTTGSSAGPAACTSAGLVPFAIGSETAGSMTYPASRCGVTALRPT 373

Query: 363 FGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDIS 422
           FGTVGRSGVMSISESLDKLGPFCRNA+DCAV+LDVIRGKDPHDLSSVES LDDPFSIDIS
Sbjct: 374 FGTVGRSGVMSISESLDKLGPFCRNAIDCAVILDVIRGKDPHDLSSVESFLDDPFSIDIS 433

Query: 423 KLTVGYLDDADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLG 482
           KLTVGYLDDADMEVVR+LSSKG NMVPFNL+YS+DSVQGI+NFTMD+DML HFDEWQR G
Sbjct: 434 KLTVGYLDDADMEVVRVLSSKGANMVPFNLTYSLDSVQGILNFTMDIDMLTHFDEWQRSG 493

Query: 483 LDDEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVC 542
           LD EYEA+DQWPTELRRARLIPAVDYVQAQRARGKL REV+ESFNVDALIGNATDWE+VC
Sbjct: 494 LDHEYEAEDQWPTELRRARLIPAVDYVQAQRARGKLRREVKESFNVDALIGNATDWERVC 553

Query: 543 MGNLVGLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATD 602
           MGNLVGLP++VVPTGFKNISNPP+TGTTRR+T ITTGIYAPPH+DHIALALAMAYQSATD
Sbjct: 554 MGNLVGLPVIVVPTGFKNISNPPATGTTRRRTAITTGIYAPPHKDHIALALAMAYQSATD 613

Query: 603 HHRAKPPIDDLGPSDVLPDPPLVSIPPRLLHL 610
           HHRA+PPIDDLGP D +P+PPLV IPPRLLHL
Sbjct: 614 HHRARPPIDDLGPHDAIPEPPLVPIPPRLLHL 640

BLAST of CSPI03G03560 vs. NCBI nr
Match: XP_011650446.1 (uncharacterized protein LOC101214338 isoform X2 [Cucumis sativus])

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 606/610 (99.34%), Postives = 607/610 (99.51%), Query Frame = 0

Query: 1   MASTPTINCFVV-FFFFFFTVSVRSSISSHFFSFPQCSWRRFTLMDSKKDSECLENMGRT 60
           MASTPTINCFVV FFFFFFTVSVRSSISSHF SFPQCSWRRFTLMDSKKDSECLENMGRT
Sbjct: 1   MASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMDSKKDSECLENMGRT 60

Query: 61  SVLKCVFRALDAGFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPE 120
           SVLKCVFRALDA FFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPE
Sbjct: 61  SVLKCVFRALDADFFNNTKRDEVLKGAEELNVPIIRANRKLVASENGGLHNPSSLVFNPE 120

Query: 121 WANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRL 180
           WANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRL
Sbjct: 121 WANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQISSQELVRIFLQRL 180

Query: 181 KRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGS 240
           KRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGS
Sbjct: 181 KRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLKDIISVPGYKTTWGS 240

Query: 241 KSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSA 300
           KSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSA
Sbjct: 241 KSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTRNPWNIEEFSTGSSA 300

Query: 301 GPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPF 360
           GPAACTSAGMVPFAIGSET+GSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPF
Sbjct: 301 GPAACTSAGMVPFAIGSETIGSMTYPAARCGVTAIRPTFGTVGRSGVMSISESLDKLGPF 360

Query: 361 CRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDADMEVVRILSSKG 420
           CRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDADMEVVRILSSKG
Sbjct: 361 CRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLDDADMEVVRILSSKG 420

Query: 421 VNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLIP 480
           VNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLIP
Sbjct: 421 VNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQDQWPTELRRARLIP 480

Query: 481 AVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPIVVVPTGFKNISNP 540
           AVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPIVVVPTGFKNISNP
Sbjct: 481 AVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLPIVVVPTGFKNISNP 540

Query: 541 PSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPIDDLGPSDVLPDPPL 600
           PSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPIDDLGPSDVLPDPPL
Sbjct: 541 PSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPIDDLGPSDVLPDPPL 600

Query: 601 VSIPPRLLHL 610
           VSIPPRLLHL
Sbjct: 601 VSIPPRLLHL 610

BLAST of CSPI03G03560 vs. NCBI nr
Match: XP_011650445.1 (uncharacterized protein LOC101214338 isoform X1 [Cucumis sativus] >KAE8650086.1 hypothetical protein Csa_011729 [Cucumis sativus])

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 606/636 (95.28%), Postives = 607/636 (95.44%), Query Frame = 0

Query: 1   MASTPTINCFVV-FFFFFFTVSVRSSISSHFFSFPQCSWRRFTLM--------------- 60
           MASTPTINCFVV FFFFFFTVSVRSSISSHF SFPQCSWRRFTLM               
Sbjct: 1   MASTPTINCFVVFFFFFFFTVSVRSSISSHFSSFPQCSWRRFTLMMGTYKKFWKLLYEKV 60

Query: 61  -----------DSKKDSECLENMGRTSVLKCVFRALDAGFFNNTKRDEVLKGAEELNVPI 120
                      DSKKDSECLENMGRTSVLKCVFRALDA FFNNTKRDEVLKGAEELNVPI
Sbjct: 61  GVHKPYSSFLQDSKKDSECLENMGRTSVLKCVFRALDADFFNNTKRDEVLKGAEELNVPI 120

Query: 121 IRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMS 180
           IRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMS
Sbjct: 121 IRANRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMS 180

Query: 181 VLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVY 240
           VLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVY
Sbjct: 181 VLELGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVY 240

Query: 241 LGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL 300
           LGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL
Sbjct: 241 LGPLHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL 300

Query: 301 AYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTA 360
           AYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSET+GSMTYPAARCGVTA
Sbjct: 301 AYDDIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETIGSMTYPAARCGVTA 360

Query: 361 IRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFS 420
           IRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFS
Sbjct: 361 IRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFS 420

Query: 421 IDISKLTVGYLDDADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEW 480
           IDISKLTVGYLDDADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEW
Sbjct: 421 IDISKLTVGYLDDADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEW 480

Query: 481 QRLGLDDEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDW 540
           QRLGLDDEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDW
Sbjct: 481 QRLGLDDEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDW 540

Query: 541 EKVCMGNLVGLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQ 600
           EKVCMGNLVGLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQ
Sbjct: 541 EKVCMGNLVGLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQ 600

Query: 601 SATDHHRAKPPIDDLGPSDVLPDPPLVSIPPRLLHL 610
           SATDHHRAKPPIDDLGPSDVLPDPPLVSIPPRLLHL
Sbjct: 601 SATDHHRAKPPIDDLGPSDVLPDPPLVSIPPRLLHL 636

BLAST of CSPI03G03560 vs. NCBI nr
Match: XP_016900589.1 (PREDICTED: glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 546/567 (96.30%), Postives = 559/567 (98.59%), Query Frame = 0

Query: 43  LMDSKKDSECLENMGRTSVLKCVFRALDAGFFNNTKRDEVLKGAEELNVPIIRANRKLVA 102
           L DSKKDSECLENMGRTS+LKCVFRA DA FFNNTKRDEVLKGAEE+NVP IRANRKLVA
Sbjct: 25  LQDSKKDSECLENMGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVA 84

Query: 103 SENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIK 162
           SENGGLHNPS LVFNPEWANEN RHKSKRFCYPPV G+KRPNNEDDIAFMSVLELGELIK
Sbjct: 85  SENGGLHNPSPLVFNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIK 144

Query: 163 TEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY 222
           TEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY
Sbjct: 145 TEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY 204

Query: 223 GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG 282
           GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG
Sbjct: 205 GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG 264

Query: 283 RTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVG 342
           RTRNPWNIEEF+TGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVG
Sbjct: 265 RTRNPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVG 324

Query: 343 RSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVG 402
           RSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVG
Sbjct: 325 RSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVG 384

Query: 403 YLDDADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEY 462
           Y+DDADMEVV+ILSSKGVNM+PFNLSYSVDSVQGI+NFTMDVDMLAHFDEWQRLGLDDEY
Sbjct: 385 YVDDADMEVVQILSSKGVNMIPFNLSYSVDSVQGIVNFTMDVDMLAHFDEWQRLGLDDEY 444

Query: 463 EAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLV 522
           EAQDQWPTELRRARLIPAVDYVQAQRARGKLIRE+RESFNVDALIGNATDWEKVCMGNLV
Sbjct: 445 EAQDQWPTELRRARLIPAVDYVQAQRARGKLIREIRESFNVDALIGNATDWEKVCMGNLV 504

Query: 523 GLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAK 582
           GLPI+VVPTGFKNISNPPSTGTTRRK TIT+GIYAPPHRDHIALALAMAYQSATDHHRA+
Sbjct: 505 GLPIIVVPTGFKNISNPPSTGTTRRKVTITSGIYAPPHRDHIALALAMAYQSATDHHRAR 564

Query: 583 PPIDDLGPSDVLPDPPLVSIPPRLLHL 610
           PPIDDLGP+DVLPDPPLVSIPPRLLHL
Sbjct: 565 PPIDDLGPNDVLPDPPLVSIPPRLLHL 591

BLAST of CSPI03G03560 vs. NCBI nr
Match: TYK19429.1 (glutamyl-tRNA(Gln) amidotransferase subunit A [Cucumis melo var. makuwa])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 536/564 (95.04%), Postives = 549/564 (97.34%), Query Frame = 0

Query: 46  SKKDSECLENMGRTSVLKCVFRALDAGFFNNTKRDEVLKGAEELNVPIIRANRKLVASEN 105
           SKKDSECLENMGRTS+LKCVFRA DA FFNNTKRDEVLKGAEE+NVP IRANRKLVASEN
Sbjct: 15  SKKDSECLENMGRTSILKCVFRAFDADFFNNTKRDEVLKGAEEVNVPTIRANRKLVASEN 74

Query: 106 GGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIKTEQ 165
           GGLHNPS LVFNPEWANEN RHKSKRFCYPPV G+KRPNNEDDIAFMSVLELGELIKTEQ
Sbjct: 75  GGLHNPSPLVFNPEWANENARHKSKRFCYPPVHGVKRPNNEDDIAFMSVLELGELIKTEQ 134

Query: 166 ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK 225
           ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK
Sbjct: 135 ISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGLK 194

Query: 226 DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR 285
           DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR
Sbjct: 195 DIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRTR 254

Query: 286 NPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRSG 345
           NPWNIEEF+TGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRSG
Sbjct: 255 NPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVGRSG 314

Query: 346 VMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYLD 405
                   DKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGY+D
Sbjct: 315 --------DKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVGYVD 374

Query: 406 DADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEYEAQ 465
           DADMEVV+ILSSKGVNM+PFNLSYSVDSVQGI+NFTMDVDMLAHFDEWQRLGLDDEYEAQ
Sbjct: 375 DADMEVVQILSSKGVNMIPFNLSYSVDSVQGIVNFTMDVDMLAHFDEWQRLGLDDEYEAQ 434

Query: 466 DQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLVGLP 525
           DQWPTELRRARLIPAVDYVQAQRARGKLIRE+RESFNVDALIGNATDWEKVCMGNLVGLP
Sbjct: 435 DQWPTELRRARLIPAVDYVQAQRARGKLIREIRESFNVDALIGNATDWEKVCMGNLVGLP 494

Query: 526 IVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAKPPI 585
           I+VVPTGFKNISNPPSTGTTRRK TIT+GIYAPPHRDHIALALAMAYQSATDHHRA+PPI
Sbjct: 495 IIVVPTGFKNISNPPSTGTTRRKVTITSGIYAPPHRDHIALALAMAYQSATDHHRARPPI 554

Query: 586 DDLGPSDVLPDPPLVSIPPRLLHL 610
           DDLGP+DVLPDPPLVSIPPRLLHL
Sbjct: 555 DDLGPNDVLPDPPLVSIPPRLLHL 570

BLAST of CSPI03G03560 vs. NCBI nr
Match: XP_038905194.1 (glutamyl-tRNA(Gln) amidotransferase subunit A [Benincasa hispida])

HSP 1 Score: 1057.7 bits (2734), Expect = 3.7e-305
Identity = 534/633 (84.36%), Postives = 567/633 (89.57%), Query Frame = 0

Query: 1   MASTPTINCFVVFFFFFFTVSVRSSISSHFFSFPQCSWRRFTLM---------------- 60
           MAS  ++     FFFFFF  +V SS   H    P  S  RFT M                
Sbjct: 1   MASIRSVIAVNFFFFFFFFFAVPSSPIFHV--LPSNSCPRFTPMMGTYKKFWKLMCEKVG 60

Query: 61  --------DSKKDSECLENMGRTSVLKCVFRALDAGFFNNTKRDEVLKGAEELNVPIIRA 120
                    SKKD+EC   +G+TS+LKCVFRA DA FFNNTK DEVLKGAEE N+P IRA
Sbjct: 61  VHSHTPPFCSKKDTEC---VGKTSLLKCVFRAFDADFFNNTKMDEVLKGAEEFNLPTIRA 120

Query: 121 NRKLVASENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLE 180
           NRKLVAS NGGLHNPS LVFNPEWANEN+RHKSKRFCYP VSG+KRP NE+DIAFMSVLE
Sbjct: 121 NRKLVASRNGGLHNPSPLVFNPEWANENIRHKSKRFCYPSVSGVKRPKNEEDIAFMSVLE 180

Query: 181 LGELIKTEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGP 240
           LGELIKT+QISSQELVRIFLQRLKRYNHVL+AVVSFTEELAYKQAKEADELFA+G YLGP
Sbjct: 181 LGELIKTKQISSQELVRIFLQRLKRYNHVLKAVVSFTEELAYKQAKEADELFARGTYLGP 240

Query: 241 LHGIPYGLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYD 300
           LHGIPYGLKDIISVPGY+TTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYD
Sbjct: 241 LHGIPYGLKDIISVPGYETTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYD 300

Query: 301 DIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRP 360
           DIWFGGRTRNPWNIEEF+TGSSAGPAACTSAGMVPFAIGSET GSMTYPAARCGVTAIRP
Sbjct: 301 DIWFGGRTRNPWNIEEFTTGSSAGPAACTSAGMVPFAIGSETAGSMTYPAARCGVTAIRP 360

Query: 361 TFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDI 420
           TFGTVGRSGVMSISESLDKLGPFCR+AVDC V+LDVIRGKDPHDLSSVESSL+DPFS+DI
Sbjct: 361 TFGTVGRSGVMSISESLDKLGPFCRSAVDCTVILDVIRGKDPHDLSSVESSLEDPFSVDI 420

Query: 421 SKLTVGYLDDADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRL 480
           SKLTVGYLDDA+MEVVR+LSSKGVNM+PFNLSYSVDSVQGI+NFTMDVDMLAHFDEWQRL
Sbjct: 421 SKLTVGYLDDAEMEVVRVLSSKGVNMIPFNLSYSVDSVQGILNFTMDVDMLAHFDEWQRL 480

Query: 481 GLDDEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKV 540
           GLDDEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLI+EVRE FNVDALIGNATDWE+V
Sbjct: 481 GLDDEYEAQDQWPTELRRARLIPAVDYVQAQRARGKLIKEVRERFNVDALIGNATDWERV 540

Query: 541 CMGNLVGLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSAT 600
           CMGNLVGLP++VVPTGFKNISNPPSTG TRR+TTITTGIYAPPH+DHIALALAMAYQSAT
Sbjct: 541 CMGNLVGLPVIVVPTGFKNISNPPSTGPTRRRTTITTGIYAPPHKDHIALALAMAYQSAT 600

Query: 601 DHHRAKPPIDDLGPSDVLPDPPLVSIPPRLLHL 610
           DHH+A+PPIDDLGP DV+ DPPLVSIPPRLLHL
Sbjct: 601 DHHKARPPIDDLGPDDVIRDPPLVSIPPRLLHL 628

BLAST of CSPI03G03560 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 873.2 bits (2255), Expect = 1.2e-253
Identity = 422/567 (74.43%), Postives = 486/567 (85.71%), Query Frame = 0

Query: 43  LMDSKKDSECLENMGRTSVLKCVFRALDAGFFNNTKRDEVLKGAEELNVPIIRANRKLVA 102
           L D   +++CL+ M         F  +D+ FFN TK  E+ KGA E+NVPI RANRKLVA
Sbjct: 103 LPDKTMEAKCLQGM---------FELIDSAFFNETKLQEIAKGATEMNVPIYRANRKLVA 162

Query: 103 SENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIK 162
           ++NGGL NPS LVFNP W  E  R + KRF YP  SG+K P +E+DIAFMSVLELGELIK
Sbjct: 163 TKNGGLENPSPLVFNPSWNREVQRVQDKRFKYPSASGVKLPRDEEDIAFMSVLELGELIK 222

Query: 163 TEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY 222
           T QI+S+ELVRI+L++LKRYNHVLEAVV++TEELAYKQAKEAD+L +QG YLGPLHGIPY
Sbjct: 223 TRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTYLGPLHGIPY 282

Query: 223 GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG 282
           GLKDI++VPGYKTTWGS SFK+Q +D+EAWVYK+LK++GAVLVAKLVTGS+AYDDIWFGG
Sbjct: 283 GLKDIVAVPGYKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAYDDIWFGG 342

Query: 283 RTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVG 342
           RTRNPWNIEEFSTGSSAGPAA TSAGMVPFAIGSET GSMTYPAARCG+TA+RPTFG+VG
Sbjct: 343 RTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCGITALRPTFGSVG 402

Query: 343 RSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVG 402
           R+GVMSISESLDKLGPFCR A DCAV+LD I+GKDP DLSS E + +DPFS+DI+KLTVG
Sbjct: 403 RTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSREIAFEDPFSVDITKLTVG 462

Query: 403 YLDDADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEY 462
           Y  DADM+VV +L SKGVNMVPF L+Y+V+SVQGI+NFTMDVDMLAHFDEWQR G +D Y
Sbjct: 463 YTKDADMKVVEVLGSKGVNMVPFELNYTVESVQGILNFTMDVDMLAHFDEWQRTGQEDLY 522

Query: 463 EAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLV 522
           EAQDQWP ELRRAR++ AVDY+QAQRARGKLIREV +SF VDA IGN TDWEKVCMGNLV
Sbjct: 523 EAQDQWPVELRRARVVTAVDYIQAQRARGKLIREVEKSFTVDAFIGNVTDWEKVCMGNLV 582

Query: 523 GLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAK 582
           GLP++V+PTGFKNI++PP T + RR+TTI  GIYAPP RDHIALAL MAYQS TD HR +
Sbjct: 583 GLPVLVIPTGFKNITDPP-TNSCRRRTTINAGIYAPPERDHIALALGMAYQSVTDAHRKR 642

Query: 583 PPIDDLGPSDVLPDPPLVSIPPRLLHL 610
           PPIDDLGP D +P+PP   IPPR LH+
Sbjct: 643 PPIDDLGPDDSIPNPPRALIPPRRLHI 659

BLAST of CSPI03G03560 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 852.8 bits (2202), Expect = 1.7e-247
Identity = 415/567 (73.19%), Postives = 479/567 (84.48%), Query Frame = 0

Query: 43  LMDSKKDSECLENMGRTSVLKCVFRALDAGFFNNTKRDEVLKGAEELNVPIIRANRKLVA 102
           L D   +++CL+ M         F  +D+ FFN TK  E+ KGA E+NVPI RANRKLVA
Sbjct: 103 LPDKTMEAKCLQGM---------FELIDSAFFNETKLQEIAKGATEMNVPIYRANRKLVA 162

Query: 103 SENGGLHNPSSLVFNPEWANENVRHKSKRFCYPPVSGIKRPNNEDDIAFMSVLELGELIK 162
           ++NGGL NPS LVFNP W  E  R + KRF YP  SG+K P +E+DIAFMSVLELGELIK
Sbjct: 163 TKNGGLENPSPLVFNPSWNREVQRVQDKRFKYPSASGVKLPRDEEDIAFMSVLELGELIK 222

Query: 163 TEQISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPY 222
           T QI+S+ELVRI+L++LKRYNHVLEAVV++TEELAYKQAKEAD+L +QG YLGPLHGIPY
Sbjct: 223 TRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTYLGPLHGIPY 282

Query: 223 GLKDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGG 282
           GLKDI++VPGYKTTWGS SFK+Q +D+EAWVYK+LK++GAVLVAKLVTGS+AYDDIWFGG
Sbjct: 283 GLKDIVAVPGYKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAYDDIWFGG 342

Query: 283 RTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRPTFGTVG 342
           RTRNPWNIEEFSTGSSAGPAA TSA       GSET GSMTYPAARCG+TA+RPTFG+VG
Sbjct: 343 RTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYPAARCGITALRPTFGSVG 402

Query: 343 RSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDDPFSIDISKLTVG 402
           R+GVMSISESLDKLGPFCR A DCAV+LD I+GKDP DLSS E + +DPFS+DI+KLTVG
Sbjct: 403 RTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSREIAFEDPFSVDITKLTVG 462

Query: 403 YLDDADMEVVRILSSKGVNMVPFNLSYSVDSVQGIINFTMDVDMLAHFDEWQRLGLDDEY 462
           Y  DADM+VV +L SKGVNMVPF L+Y+V+SVQGI+NFTMDVDMLAHFDEWQR G +D Y
Sbjct: 463 YTKDADMKVVEVLGSKGVNMVPFELNYTVESVQGILNFTMDVDMLAHFDEWQRTGQEDLY 522

Query: 463 EAQDQWPTELRRARLIPAVDYVQAQRARGKLIREVRESFNVDALIGNATDWEKVCMGNLV 522
           EAQDQWP ELRRAR++ AVDY+QAQRARGKLIREV +SF VDA IGN TDWEKVCMGNLV
Sbjct: 523 EAQDQWPVELRRARVVTAVDYIQAQRARGKLIREVEKSFTVDAFIGNVTDWEKVCMGNLV 582

Query: 523 GLPIVVVPTGFKNISNPPSTGTTRRKTTITTGIYAPPHRDHIALALAMAYQSATDHHRAK 582
           GLP++V+PTGFKNI++PP T + RR+TTI  GIYAPP RDHIALAL MAYQS TD HR +
Sbjct: 583 GLPVLVIPTGFKNITDPP-TNSCRRRTTINAGIYAPPERDHIALALGMAYQSVTDAHRKR 642

Query: 583 PPIDDLGPSDVLPDPPLVSIPPRLLHL 610
           PPIDDLGP D +P+PP   IPPR LH+
Sbjct: 643 PPIDDLGPDDSIPNPPRALIPPRRLHI 652

BLAST of CSPI03G03560 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 115.2 bits (287), Expect = 1.9e-25
Identity = 71/234 (30.34%), Postives = 118/234 (50.43%), Query Frame = 0

Query: 165 QISSQELVRIFLQRLKRYNHVLEAVVSFTEELAYKQAKEADELFAQGVYLGPLHGIPYGL 224
           + ++ E+ + +L R++     L+  +  +E +  K A+E D+  A+G  LGPL G+  G+
Sbjct: 57  ETTAVEIAKSYLSRIRLTEPQLKCFLHVSENV-LKDAQEIDQRIAKGEELGPLAGVLIGV 116

Query: 225 KDIISVPGYKTTWGSKSFKEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLAYDDIWFGGRT 284
           KD I   G  +T  S+  +      +A   K++K  G ++V     G    D+   G  T
Sbjct: 117 KDNICTQGMPSTAASRILEHYRPPFDATAVKKIKELGGIVV-----GKTNMDEFGMGSTT 176

Query: 285 R--------NPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGVTAIRP 344
                    NPW++     GSS G AA  +A     ++GS+T GS+  PA+ CGV  ++P
Sbjct: 177 EASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKP 236

Query: 345 TFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESSLDD 391
           T+G V R G+M+ + SLD +G F     D  ++L  I G D  D +S +  + +
Sbjct: 237 TYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQDVPE 284

BLAST of CSPI03G03560 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 79.0 bits (193), Expect = 1.5e-14
Identity = 57/187 (30.48%), Postives = 88/187 (47.06%), Query Frame = 0

Query: 217 LHGIPYGLKDIISVPGYKTTWGSKSF--KEQVIDVEAWVYKQLKSAGAVLVAKLVTGSLA 276
           L G+ + +KDI  V G  T +G+  +          A V   L  AGA  +   +   +A
Sbjct: 28  LQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAATSTAPVVSSLLEAGATALGITIMDEMA 87

Query: 277 YD---DIWFGGRTRNPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAARCGV 336
           Y    +    G  RNP   +    GSS+G A   +A +V F+IG++T GS+  PA+ CG+
Sbjct: 88  YSINGENAHYGTPRNPIAFDRVPGGSSSGSAVAVAARLVDFSIGTDTGGSVRVPASYCGI 147

Query: 337 TAIRPTFGTVGRSGVMSISESLDKLGPFCRNAVDCAVVLDVIRGKDPHDLSSVESS---- 395
              RP+ G V   G+  +++S D +G F R+      V  V+     H L+ +E S    
Sbjct: 148 FGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTATLKRVGCVL--LQQHHLNPIEPSQLII 207

BLAST of CSPI03G03560 vs. TAIR 10
Match: AT3G17970.1 (translocon at the outer membrane of chloroplasts 64-III )

HSP 1 Score: 73.2 bits (178), Expect = 8.3e-13
Identity = 43/155 (27.74%), Postives = 70/155 (45.16%), Query Frame = 0

Query: 216 PLHGIPYGLKDIISVPGYKTTWGSKSF--KEQVIDVEAWVYKQLKSAGAVLVAKLVTGSL 275
           PL G+ + + D+  + GY T +G   +    +     + V   L   GA  V K V    
Sbjct: 57  PLTGLTFAVSDVFDITGYVTGFGHPDWVRTHEAASSTSPVVSTLVEGGATCVGKTVVDEF 116

Query: 276 AYDDIWFGGRTR------NPWNIEEFSTGSSAGPAACTSAGMVPFAIGSETVGSMTYPAA 335
           A+      G  +      NP        G+ +G A   +   V FA+G +TVG +  PA 
Sbjct: 117 AFS---ISGENKHYDSPTNPAAPTRIPGGACSGAAVAVATNAVDFALGIDTVGGVRVPAG 176

Query: 336 RCGVTAIRPTFGTVGRSGVMSISESLDKLGPFCRN 363
            CGV   + ++G +  +G++ +S SLD +G F R+
Sbjct: 177 YCGVLGFKSSYGAISNTGIIPVSSSLDSVGWFARD 208