CSPI03G03060 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI03G03060
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Chr3: 2504034 .. 2504609 (-)
RNA-Seq Expression	CSPI03G03060
Synteny	CSPI03G03060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATCCTCATACTTTCCTCTTCCAGTCAAAGCCATTCTTCTCATCCTCCTAATAAACCAATCAAACATTGCAAATTCTCAACCAATAAATGACACCCAATTCATCAAAACCACTTGTCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCTGCCGCCACCATTCACAGTAGCTGTCATCTAATGACCGTCGCTGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGATCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGATAGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTGTGGAGGAGTTGAAGGATAATAACAAGAGTCGATCCGAAACTGAAGATATTCGTACGTGGGTTAGCGCTGCTCTAACAGACGACGACACGTGTATGGATGGGTTGGTTGGAGATGCCATGAATGGCAACGTAAAGGAATCCATAAAGGAAATGGTGGTGAATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTGTAAGTTTGTTGAAGTGA

mRNA sequence

ATGAAATCCTCATACTTTCCTCTTCCAGTCAAAGCCATTCTTCTCATCCTCCTAATAAACCAATCAAACATTGCAAATTCTCAACCAATAAATGACACCCAATTCATCAAAACCACTTGTCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCTGCCGCCACCATTCACAGTAGCTGTCATCTAATGACCGTCGCTGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGATCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGATAGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTGTGGAGGAGTTGAAGGATAATAACAAGAGTCGATCCGAAACTGAAGATATTCGTACGTGGGTTAGCGCTGCTCTAACAGACGACGACACGTGTATGGATGGGTTGGTTGGAGATGCCATGAATGGCAACGTAAAGGAATCCATAAAGGAAATGGTGGTGAATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTGTAAGTTTGTTGAAGTGA

Coding sequence (CDS)

ATGAAATCCTCATACTTTCCTCTTCCAGTCAAAGCCATTCTTCTCATCCTCCTAATAAACCAATCAAACATTGCAAATTCTCAACCAATAAATGACACCCAATTCATCAAAACCACTTGTCAATCCACACCCTATCCTGACCTCTGCCTCTCCTCTCTCTCCGACTCTGCCGCCACCATTCACAGTAGCTGTCATCTAATGACCGTCGCTGCCCTCACCGTTGCTCTCACACACACGCGCTCTACCTCATCGGCGATCGAGAGCCTGGCCAAGTCGTCGAATGCCTTGACGCCGCGAGATAGTTATGTCATACGTGATTGTATTGAAGAATTTGGAGACTCAGTTGAAGAACTAAAGATGGCTGTGGAGGAGTTGAAGGATAATAACAAGAGTCGATCCGAAACTGAAGATATTCGTACGTGGGTTAGCGCTGCTCTAACAGACGACGACACGTGTATGGATGGGTTGGTTGGAGATGCCATGAATGGCAACGTAAAGGAATCCATAAAGGAAATGGTGGTGAATGTGGCTCAATTGACAAGCATTGCTCTTTCACTTGTAAGTTTGTTGAAGTGA

Protein sequence

MKSSYFPLPVKAILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLVSLLK*

Homology

[Close]

BLAST of CSPI03G03060 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.9e-23
Identity = 77/193 (39.90%), Postives = 110/193 (56.99%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATI 60
           MK S   L   A+L+IL       A++ P N  QFIKT+C  T YP +C  SLS  A TI
Sbjct: 1   MKLSKSTLVFSALLVILA-----AASAAPAN--QFIKTSCTLTTYPAVCEQSLSAYAKTI 60

Query: 61  HSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKM 120
            ++   +   AL V+LT T+   + ++ L K    L  R    I DC+EE  DS++ +  
Sbjct: 61  QNNPQELASTALQVSLTRTQQAQTFMKRLNK-FKGLKARQYAAIHDCLEEVEDSLDRVSR 120

Query: 121 AVEELKD-----NNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVN 180
           + +E+K+      N       ++ TWVSAALTD+ TCMDG  G  M+G +KES++  VV 
Sbjct: 121 SCDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVA 180

Query: 181 VAQLTSIALSLVS 189
           VA++TS AL+LV+
Sbjct: 181 VARVTSNALALVN 185

[Close]

BLAST of CSPI03G03060 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.1e-21
Identity = 73/183 (39.89%), Postives = 107/183 (58.47%), Query Frame = 0


Query: 13  ILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATI-HSSCHLMTVAA 72
           ILL + I QS+ A        QFI ++CQ+T YP LC+ +LS  A  I H++   +   A
Sbjct: 11  ILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTA 70

Query: 73  LTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEEL-KDNNK 132
           L ++L   +S +  +  L K +     R+   I+DCIE  G+SV+ L  +V+EL +  + 
Sbjct: 71  LIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHA 130

Query: 133 SRSE-----TEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALS 189
             SE       +++TWVSAALTD+ TC+DG    AM G VK  I+  VV+VAQ+TS AL+
Sbjct: 131 VASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALA 190

[Close]

BLAST of CSPI03G03060 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.3e-21
Identity = 65/187 (34.76%), Postives = 105/187 (56.15%), Query Frame = 0


Query: 13  ILLILLINQSNIANSQPI-----NDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLM 72
           IL +L ++ + I  ++ +      DT+FIK +C++T YPD C  SLS  A+ I      +
Sbjct: 10  ILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKL 69

Query: 73  TVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD 132
              AL V++   +S  + +  +      +T R    + DC+EE GD+V+ L  +++ELK 
Sbjct: 70  AETALAVSIARAKSAKTYVSEMT-DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKH 129

Query: 133 NNKSRSETE------DIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTS 189
             +  S  +      ++RTW SAALTD+  CMDG  G AM G +K  I+  +V+VA+ TS
Sbjct: 130 LEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETS 189

[Close]

BLAST of CSPI03G03060 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.8e-20
Identity = 71/192 (36.98%), Postives = 108/192 (56.25%), Query Frame = 0


Query: 3   SSYFPLPVKAILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHS 62
           SS  P P  +  L  L   S+ +      +  +IKT+C  T Y  +C +SLS  A+TI S
Sbjct: 115 SSPPPPPPSSSPLSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRS 174

Query: 63  SCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAV 122
           +   + V AL + L+  +S S  +++++     LT  +   + DC+EE GDSV  L+ ++
Sbjct: 175 NPQKLAVIALNLTLSSAKSASKFVKNIS-HGGGLTRLEVVAVADCVEEIGDSVTSLQDSI 234

Query: 123 EEL-----KDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVA 182
            EL     KD+ K      D+ TWVSAALT+DDTCMDG     +   VK+ ++  VV VA
Sbjct: 235 RELDSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVA 294

Query: 183 QLTSIALSLVSL 190
           +LTS AL+L+++
Sbjct: 295 RLTSNALALINM 303

[Close]

BLAST of CSPI03G03060 vs. ExPASy Swiss-Prot
Match: Q9SB38 (Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.0e-18
Identity = 56/189 (29.63%), Postives = 104/189 (55.03%), Query Frame = 0


Query: 10  VKAILLILLINQSNIANSQPI------NDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSS 69
           V ++ L++ IN SN   +         N T ++KT C ST YP +C + LS  ++TI S 
Sbjct: 6   VLSLTLMVFINSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSSTIKSD 65

Query: 70  CHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVE 129
              +   +L + +   ++ +  + +L + + A    +  +++DC++E  D+++ELK AV 
Sbjct: 66  PIKLCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDELKQAVA 125

Query: 130 ELKDNNKSRSETED----IRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 189
           E+K        TE+    ++TWVS+ALTD+ TC DG     +N   K+ +K+ +  +++ 
Sbjct: 126 EMKYVRGGGKTTEEHLKNVKTWVSSALTDEGTCTDGFEEGRVNVETKKKVKKAISELSKT 185

[Close]

BLAST of CSPI03G03060 vs. ExPASy TrEMBL
Match: A0A0A0L1P3 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 5.7e-93
Identity = 191/192 (99.48%), Postives = 191/192 (99.48%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLILLINQSN-IANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           MKSSYFPLPVKAILLILLINQSN IANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT
Sbjct: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK
Sbjct: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120

Query: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180
           MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL
Sbjct: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180

Query: 181 TSIALSLVSLLK 192
           TSIALSLVSLLK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of CSPI03G03060 vs. ExPASy TrEMBL
Match: A0A1S3BLP3 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 2.0e-85
Identity = 175/192 (91.15%), Postives = 184/192 (95.83%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLI-LLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           MKSSYFPLPVKAILL+ +LINQSNIANSQP NDTQFIKTTCQSTPYPDLCLSSLSDSAA 
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           IHSS  LMT+AALTVALTHTRSTSSA+ESLAKSSNALTPR+ YVIRDCIEEFGDSVEELK
Sbjct: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120

Query: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180
           MA+EELKDNNK+R ETEDIRTWVSAALTDDDTCMDGLVGDAMNGN+KESIK MVV+VAQL
Sbjct: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180

Query: 181 TSIALSLVSLLK 192
           TSIALSLVSLLK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of CSPI03G03060 vs. ExPASy TrEMBL
Match: A0A6J1E6H9 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 2.2e-60
Identity = 131/184 (71.20%), Postives = 152/184 (82.61%), Query Frame = 0


Query: 8   LPVKAILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLM 67
           LP+ AI+ ++LINQS    +QPINDTQFIKTTCQ+TPYPDLCLSSLS  A+ I  S  LM
Sbjct: 6   LPITAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLM 65

Query: 68  TVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD 127
           T+AAL VALTHT   SS + SLAK SNA+T R+ Y + DCIEEFGDSVEELKMAVE LK 
Sbjct: 66  TIAALAVALTHTHDASSTVTSLAK-SNAITTREGYALSDCIEEFGDSVEELKMAVEALKG 125

Query: 128 NNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLV 187
           N+K+R +TEDI+TW+SAALTDD+TCMDG  GDAMNG+VKES+K MVV+VAQLTSIALSLV
Sbjct: 126 NDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLV 185

Query: 188 SLLK 192
             LK
Sbjct: 186 RQLK 188

[Close]

BLAST of CSPI03G03060 vs. ExPASy TrEMBL
Match: A0A6J1DFW8 (pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 PE=4 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 3.5e-58
Identity = 132/191 (69.11%), Postives = 155/191 (81.15%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATI 60
           M+ SYFPLP+ AILLI+LIN SN      I+DTQFIK TCQ+TPYPDLC SSLS  A ++
Sbjct: 1   MEDSYFPLPITAILLIILINLSNAT----IDDTQFIKATCQTTPYPDLCFSSLSADAVSV 60

Query: 61  HSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKM 120
           H S   MT+AALTVALTHTRS SS + SLAK SNAL P++++ +RDCIEE GDSVEEL+ 
Sbjct: 61  HGSLRSMTIAALTVALTHTRSASSMVASLAK-SNALAPQETWALRDCIEELGDSVEELQS 120

Query: 121 AVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLT 180
           +V ELKD + SR ET+DIRTWVSAALTDDDTCM+GLVGD   G VKES++ +VV+VAQLT
Sbjct: 121 SVAELKDGDGSRPETDDIRTWVSAALTDDDTCMEGLVGD--GGGVKESVRGLVVDVAQLT 180

Query: 181 SIALSLVSLLK 192
           SIALSLVS LK
Sbjct: 181 SIALSLVSHLK 184

[Close]

BLAST of CSPI03G03060 vs. ExPASy TrEMBL
Match: A0A5D3D7I0 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001400 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 9.9e-53
Identity = 115/125 (92.00%), Postives = 122/125 (97.60%), Query Frame = 0


Query: 67  MTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELK 126
           MT+AALTVALTHTRSTSSA+ESLAKSSNALTPR+ YVIRDCIEEFGDSVEELKMA+EELK
Sbjct: 1   MTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELKMAIEELK 60

Query: 127 DNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSL 186
           DNNK+R ETEDIRTWVSAALTDDDTCMDGLVGDAMNGN+KESIK MVV+VAQLTSIALSL
Sbjct: 61  DNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQLTSIALSL 120

Query: 187 VSLLK 192
           VSLLK
Sbjct: 121 VSLLK 125

[Close]

BLAST of CSPI03G03060 vs. NCBI nr
Match: XP_004147977.1 (21 kDa protein [Cucumis sativus])

HSP 1 Score: 350.1 bits (897), Expect = 1.2e-92
Identity = 191/192 (99.48%), Postives = 191/192 (99.48%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLILLINQSN-IANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           MKSSYFPLPVKAILLILLINQSN IANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT
Sbjct: 1   MKSSYFPLPVKAILLILLINQSNIIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK
Sbjct: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120

Query: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180
           MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL
Sbjct: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180

Query: 181 TSIALSLVSLLK 192
           TSIALSLVSLLK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of CSPI03G03060 vs. NCBI nr
Match: XP_008448897.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 325.1 bits (832), Expect = 4.1e-85
Identity = 175/192 (91.15%), Postives = 184/192 (95.83%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLI-LLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAAT 60
           MKSSYFPLPVKAILL+ +LINQSNIANSQP NDTQFIKTTCQSTPYPDLCLSSLSDSAA 
Sbjct: 1   MKSSYFPLPVKAILLLMILINQSNIANSQPTNDTQFIKTTCQSTPYPDLCLSSLSDSAAA 60

Query: 61  IHSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELK 120
           IHSS  LMT+AALTVALTHTRSTSSA+ESLAKSSNALTPR+ YVIRDCIEEFGDSVEELK
Sbjct: 61  IHSSYRLMTIAALTVALTHTRSTSSAVESLAKSSNALTPREGYVIRDCIEEFGDSVEELK 120

Query: 121 MAVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQL 180
           MA+EELKDNNK+R ETEDIRTWVSAALTDDDTCMDGLVGDAMNGN+KESIK MVV+VAQL
Sbjct: 121 MAIEELKDNNKTRFETEDIRTWVSAALTDDDTCMDGLVGDAMNGNIKESIKAMVVDVAQL 180

Query: 181 TSIALSLVSLLK 192
           TSIALSLVSLLK
Sbjct: 181 TSIALSLVSLLK 192

[Close]

BLAST of CSPI03G03060 vs. NCBI nr
Match: XP_038876812.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 283.1 bits (723), Expect = 1.8e-72
Identity = 152/191 (79.58%), Postives = 168/191 (87.96%), Query Frame = 0


Query: 1   MKSSYFPLPVKAILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATI 60
           M+SSY PLP+KAILLI+LINQSNIA SQPINDTQFIKTTCQSTPYP LCLSSLS  AATI
Sbjct: 1   MESSYLPLPIKAILLIILINQSNIAKSQPINDTQFIKTTCQSTPYPSLCLSSLSADAATI 60

Query: 61  HSSCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKM 120
           H S  LMT+AALTVALT TRS SS I+SLAK SN LTPR+ YV+ DCIEEFGDS+EELKM
Sbjct: 61  HGSYRLMTIAALTVALTRTRSASSVIQSLAK-SNVLTPREGYVVSDCIEEFGDSIEELKM 120

Query: 121 AVEELKDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLT 180
           A+ ELKDN+K R E EDIRTWVSAALTDDDTCMDG VGD MN +VKES+K++VV++ QLT
Sbjct: 121 AIVELKDNDKMRFEIEDIRTWVSAALTDDDTCMDGFVGDTMNDSVKESMKKLVVDIVQLT 180

Query: 181 SIALSLVSLLK 192
           SIALSLVSLLK
Sbjct: 181 SIALSLVSLLK 190

[Close]

BLAST of CSPI03G03060 vs. NCBI nr
Match: XP_023551923.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 243.0 bits (619), Expect = 2.0e-60
Identity = 131/184 (71.20%), Postives = 153/184 (83.15%), Query Frame = 0


Query: 8   LPVKAILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLM 67
           LP+ AIL ++LINQS    +QPINDTQFIKTTCQ+TPYPDLCLSSLS  A+ IH S  LM
Sbjct: 6   LPITAILFMILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSADASAIHGSHRLM 65

Query: 68  TVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD 127
           T+AAL VALTHT   SS + SLAK SNALTPR+ Y + DCIEEFGDS+EELKMAVEELK 
Sbjct: 66  TIAALAVALTHTHDASSTVTSLAK-SNALTPREGYALSDCIEEFGDSIEELKMAVEELKG 125

Query: 128 NNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLV 187
           N+K+R + +DI+TWVSAALTDD+TCMDG   DAMNG+VKES++ +VV+VAQLTSIALSLV
Sbjct: 126 NDKTRLDIKDIQTWVSAALTDDNTCMDGFAEDAMNGSVKESVRALVVDVAQLTSIALSLV 185

Query: 188 SLLK 192
             LK
Sbjct: 186 RQLK 188

[Close]

BLAST of CSPI03G03060 vs. NCBI nr
Match: XP_022923321.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 241.9 bits (616), Expect = 4.6e-60
Identity = 131/184 (71.20%), Postives = 152/184 (82.61%), Query Frame = 0


Query: 8   LPVKAILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLM 67
           LP+ AI+ ++LINQS    +QPINDTQFIKTTCQ+TPYPDLCLSSLS  A+ I  S  LM
Sbjct: 6   LPITAIVFVILINQSYTVATQPINDTQFIKTTCQTTPYPDLCLSSLSPDASAIRCSHRLM 65

Query: 68  TVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD 127
           T+AAL VALTHT   SS + SLAK SNA+T R+ Y + DCIEEFGDSVEELKMAVE LK 
Sbjct: 66  TIAALAVALTHTHDASSTVTSLAK-SNAITTREGYALSDCIEEFGDSVEELKMAVEALKG 125

Query: 128 NNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLV 187
           N+K+R +TEDI+TW+SAALTDD+TCMDG  GDAMNG+VKES+K MVV+VAQLTSIALSLV
Sbjct: 126 NDKTRLDTEDIQTWISAALTDDNTCMDGFAGDAMNGSVKESMKAMVVDVAQLTSIALSLV 185

Query: 188 SLLK 192
             LK
Sbjct: 186 RQLK 188

[Close]

BLAST of CSPI03G03060 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 116.3 bits (290), Expect = 2.7e-26
Identity = 70/178 (39.33%), Postives = 112/178 (62.92%), Query Frame = 0


Query: 13  ILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLMTVAAL 72
           I ++ L++ +    +    +T+F+K++C  T YP LC SSLS  A+ I +S  LM  AAL
Sbjct: 21  IAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAAL 80

Query: 73  TVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKDNNKSR 132
            + L   + TS+ +  L  S++ L P++   +RDC+EE GD++EEL+ ++ E+   + S 
Sbjct: 81  NITLASAKVTSAMMVRL--SNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSN 140

Query: 133 SET--EDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLVS 189
            E    DI+TWVSAALTD +TC DG  G+ M+G VK  ++  ++ +A LTS AL+L++
Sbjct: 141 YEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALIN 196

[Close]

BLAST of CSPI03G03060 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 8.1e-23
Identity = 73/183 (39.89%), Postives = 107/183 (58.47%), Query Frame = 0


Query: 13  ILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATI-HSSCHLMTVAA 72
           ILL + I QS+ A        QFI ++CQ+T YP LC+ +LS  A  I H++   +   A
Sbjct: 11  ILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTA 70

Query: 73  LTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEEL-KDNNK 132
           L ++L   +S +  +  L K +     R+   I+DCIE  G+SV+ L  +V+EL +  + 
Sbjct: 71  LIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHA 130

Query: 133 SRSE-----TEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALS 189
             SE       +++TWVSAALTD+ TC+DG    AM G VK  I+  VV+VAQ+TS AL+
Sbjct: 131 VASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALA 190

[Close]

BLAST of CSPI03G03060 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 3.1e-22
Identity = 65/187 (34.76%), Postives = 105/187 (56.15%), Query Frame = 0


Query: 13  ILLILLINQSNIANSQPI-----NDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLM 72
           IL +L ++ + I  ++ +      DT+FIK +C++T YPD C  SLS  A+ I      +
Sbjct: 10  ILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKL 69

Query: 73  TVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEELKD 132
              AL V++   +S  + +  +      +T R    + DC+EE GD+V+ L  +++ELK 
Sbjct: 70  AETALAVSIARAKSAKTYVSEMT-DYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKH 129

Query: 133 NNKSRSETE------DIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTS 189
             +  S  +      ++RTW SAALTD+  CMDG  G AM G +K  I+  +V+VA+ TS
Sbjct: 130 LEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETS 189

[Close]

BLAST of CSPI03G03060 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 100.1 bits (248), Expect = 2.0e-21
Identity = 71/192 (36.98%), Postives = 108/192 (56.25%), Query Frame = 0


Query: 3   SSYFPLPVKAILLILLINQSNIANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHS 62
           SS  P P  +  L  L   S+ +      +  +IKT+C  T Y  +C +SLS  A+TI S
Sbjct: 115 SSPPPPPPSSSPLSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRS 174

Query: 63  SCHLMTVAALTVALTHTRSTSSAIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAV 122
           +   + V AL + L+  +S S  +++++     LT  +   + DC+EE GDSV  L+ ++
Sbjct: 175 NPQKLAVIALNLTLSSAKSASKFVKNIS-HGGGLTRLEVVAVADCVEEIGDSVTSLQDSI 234

Query: 123 EEL-----KDNNKSRSETEDIRTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVA 182
            EL     KD+ K      D+ TWVSAALT+DDTCMDG     +   VK+ ++  VV VA
Sbjct: 235 RELDSINYKDSAKFEMVMSDVETWVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVA 294

Query: 183 QLTSIALSLVSL 190
           +LTS AL+L+++
Sbjct: 295 RLTSNALALINM 303

[Close]

BLAST of CSPI03G03060 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 3.4e-21
Identity = 63/170 (37.06%), Postives = 99/170 (58.24%), Query Frame = 0


Query: 25  ANSQPINDTQFIKTTCQSTPYPDLCLSSLSDSAATIHSSCHLMTVAALTVALTHTRSTSS 84
           A+ + IN   FI+++C++T YP LC+ SLS  A  I +S   +   A+ V L+  +ST  
Sbjct: 31  ASKKAIN---FIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKL 90

Query: 85  AIESLAKSSNALTPRDSYVIRDCIEEFGDSVEELKMAVEEL------KDNNKSRSETEDI 144
            +  L +    L  R+   I+DC+EE  D+V+ L  +V+EL      KD ++      + 
Sbjct: 91  FVSRLTR-MKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNA 150

Query: 145 RTWVSAALTDDDTCMDGLVGDAMNGNVKESIKEMVVNVAQLTSIALSLVS 189
           +TW SAALTD++TC DG  G  M+G +K S++  ++NV   TS ALSL++
Sbjct: 151 QTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLIN 196

The following BLAST results are available for this feature:

BLAST of CSPI03G03060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	7.9e-23	39.90	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI72	1.1e-21	39.89	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9SB37	4.3e-21	34.76	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI74	2.8e-20	36.98	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9SB38	2.0e-18	29.63	Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1	[more]

BLAST of CSPI03G03060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1P3	5.7e-93	99.48	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G035340 PE=4 S...	[more]
A0A1S3BLP3	2.0e-85	91.15	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103490923 PE=4 SV=1	[more]
A0A6J1E6H9	2.2e-60	71.20	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431052 PE=4 SV=1	[more]
A0A6J1DFW8	3.5e-58	69.11	pectinesterase inhibitor 7-like OS=Momordica charantia OX=3673 GN=LOC111020439 P...	[more]
A0A5D3D7I0	9.9e-53	92.00	21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G...	[more]

BLAST of CSPI03G03060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147977.1	1.2e-92	99.48	21 kDa protein [Cucumis sativus]	[more]
XP_008448897.1	4.1e-85	91.15	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
XP_038876812.1	1.8e-72	79.58	21 kDa protein-like [Benincasa hispida]	[more]
XP_023551923.1	2.0e-60	71.20	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_022923321.1	4.6e-60	71.20	21 kDa protein-like [Cucurbita moschata]	[more]

BLAST of CSPI03G03060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	2.7e-26	39.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	8.1e-23	39.89	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	3.1e-22	34.76	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	2.0e-21	36.98	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	3.4e-21	37.06	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 108..135
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 18..188
None	No IPR available	PANTHER	PTHR31080:SF161	21 KDA PROTEIN-LIKE	coord: 18..188
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 38..190 e-value: 2.02412E-35 score: 119.85
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 31..186 e-value: 1.4E-43 score: 160.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 13..188 e-value: 8.6E-33 score: 111.1
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 35..186 e-value: 1.7E-35 score: 122.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 30..191 e-value: 1.5E-38 score: 134.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 34..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G03060.1	CSPI03G03060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity